Brandt, C, Radbruch, A, Worm, M, Baumgrass, R
No abstract available.
Bendfeldt, H, Frischbutter, S, Kroeger, S, Radbruch, A, Baumgrass, R
No abstract available.
Inhibition of calcineurin-NFAT signaling by the pyroazolopyrimidine compound NCI3 (2007)
Sieber, M., Karanik, M., Brandt, C., Blex, C., Podtschaske, M., Erdmann, F., ...
Membrane glucocorticoid receptors (mGCR) on monocytes are up-regulated after vaccination (2007)
Spies, C. M., Bartholome, B., Berki, T., Radbruch, A., Scheffold, A., ...
The systemic and SmD183-119-autoantigen-specific cytokine memory of Th cells in SLE patients (2007)
Langer, S., Langnickel, D., Enghard, P., Undeutsch, R., Burmester, G. R., Hiepe, F., ...
Objectives. The aim of the study was to analyse the cytokine memory of T-cells derived from systemic lupus erythematosus (SLE) patients and healthy donors enriched for autoantigen-specific T-cells by...
The systemic and SmD183-119-autoantigen-specific cytokine memory of Th cells in SLE patients (2006)
Langer, S., Langnickel, D., Enghard, P., Undeutsch, R., Burmester, G. R., Hiepe, F., ...
Objectives. The aim of the study was to analyse the cytokine memory of T-cells derived from systemic lupus erythematosus (SLE) patients and healthy donors enriched for autoantigen-specific T-cells by...
Membrane glucocorticoid receptors (mGCR) on monocytes are up-regulated after vaccination (2006)
Spies, C. M., Bartholome, B., Berki, T., Radbruch, A., Scheffold, A., ...
The systemic and SmD183-119-autoantigen-specific cytokine memory of Th cells in SLE patients (2006)
Langer, S., Langnickel, D., Enghard, P., Undeutsch, R., Burmester, G. R., Hiepe, F., ...
Objectives. The aim of the study was to analyse the cytokine memory of T-cells derived from systemic lupus erythematosus (SLE) patients and healthy donors enriched for autoantigen-specific T-cells by...
Membrane glucocorticoid receptors (mGCR) on monocytes are up-regulated after vaccination (2006)
Spies, C. M., Bartholome, B., Berki, T., Radbruch, A., Scheffold, A., ...
Gene expression in chronically activated Th cells (2005)
Niesner, U, Albrecht, I, Baumgrass, R, Janitz, M, Lehrach, H, Haeupl, T, ...
No abstract available.
Type 1 regulatory T cells in treatment of murine lupus (2005)
Undeutsch, R, Humrich, J, Hahn, BH, Hiepe, F, Burmester, G, Radbruch, A, ...
No abstract available.
Alexander, T, Massenkeil, G, Gromnica-Ihle, E, Burmester, GR, Radbruch, A, Arnold, R, ...
No abstract available.
Häupl, T, Grützkau, A, Grün, J, Krenn, V, Rudwaleit, M, Stuhlmüller, B, ...
No abstract available.
Protective role of interferon gamma in murine antigen-induced arthritis (2004)
Hückel, M, Irmler, I, Simon, J, Radbruch, A, Scheffold, A, Bräuer, R
No abstract available.
GATA-3 in human T helper cell type 2 development (2004)
Skapenko, A, Leipe, J, Niesner, U, Devriendt, K, Beetz, R, Radbruch, A, ...
No abstract available.
Hoyer, BF, Moser, K, Hauser, AE, Peddinghaus, A, Voigt, C, Eilat, D, ...
No abstract available.
Muehlinghaus, G, Cigliano, L, Leyendeckers, H, Arce, S, Radbruch, A, Manz, RA
No abstract available.
Tripmacher, R, Gaber, T, Burmester, GR, Radbruch, A, Scheffold, A
No abstract available.
Characterization of kidney infiltrating T cells in the NZB/W F1 lupus model (2004)
Enghard, P, Langnickel, D, Burmester, GR, Hiepe, F, Radbruch, A, Riemekasten, G
No abstract available.
Monocyte-specific gene signatures in rheumatoid arthritis (2004)
Grützkau, A, Grün, J, Rudwaleit, M, Goldschmidt, L, Burmester, G-R, Radbruch, A, ...
No abstract available.
Häupl, T, Grützkau, A, Grün, J, Baumgrass, R, Janitz, M, Stuhlmüller, B, ...
No abstract available.
Alexander, T, Massenkeil, G, Burmester, GR, Radbruch, A, Arnold, R, Hiepe, F, ...
No abstract available.
Hiepe, F, Manz, R, Hoyer, B, Thiel, A, Alexander, T, Burmester, G-R, ...
No abstract available.
GATA-3 in human T cell helper type 2 development (2004)
Leipe, J, Niesner, U, Beetz, R, Radbruch, A, ...
The delineation of the in vivo role of GATA-3 in human T cell differentiation is a critical step in the understanding of molecular mechanisms directing human immune responses. We examined T cell...
Analysis of B-cell subpopulations in systemic lupus erythematosus (2003)
Dorner, T, Jacobi, A, Odendahl, M, Burmester, GR, Radbruch, A, Valet, G, ...
No abstract available.
Radbruch, A, Thiel, A, Kimmig, S, Alexander, T, Gromnica-Ihle, E, Hiepe, F, ...
No abstract available.
Regulatory T cells in experimental arthritis (2003)
Bräuer, R, Frey, O, Petrow, PK, Scheffold, A, Radbruch, A
No abstract available.
Jacobi,A. M., Odendahl,M., Reiter,K., Bruns,A., Burmester,G. R., Radbruch,A., ...
Objective. Disease activity in systemic lupus erythematosus (SLE) is usually assessed with complex disease activity scores comprising a variety of different parameters. In order to determine whether...
Plasma-cell like morphotype with loss of CD3 expression by Th1 cytokine producing cells (2003)
Page, G, Sattler, A, Thiel, A, Radbruch, A, Miossec, P
No abstract available.
Tykocinski, L, Hajkova, P, Stamm, T, Sözeri, O, Löhning, M, Friedrich, B, ...
No abstract available.
Analysis of B-cell subpopulations in systemic lupus erythematosus (2003)
Dorner,T., Jacobi,A., Odendahl,M., Burmester,G. R., Radbruch,A., Valet,G., ...
Jacobi, A. M., Odendahl, M., Reiter, K., Bruns, A., Burmester, G. R., Radbruch, A., ...
Objective. Disease activity in systemic lupus erythematosus (SLE) is usually assessed with complex disease activity scores comprising a variety of different parameters. In order to determine whether...
Analysis of B-cell subpopulations in systemic lupus erythematosus (2003)
Dorner, T., Jacobi, A., Odendahl, M., Burmester, G. R., Radbruch, A., Valet, G., ...
Zou, J., Zhang, Y., Thiel, A., Rudwaleit, M., Radbruch, A., ...
Objectives. Based on their HLA association, both ankylosing spondylitis (AS) and rheumatoid arthritis (RA) seem to be T-cell-driven diseases in which the autoantigens remain to be defined. One...
Zou, J., Zhang, Y., Thiel, A., Rudwaleit, M., Radbruch, A., ...
Objectives. Based on their HLA association, both ankylosing spondylitis (AS) and rheumatoid arthritis (RA) seem to be T-cell-driven diseases in which the autoantigens remain to be defined. One...
Thiel, AT, Thiel, A, Alexander, T, Rosen, O, Gromnica-Ihle, E, Burmester, GR, ...
No abstract available.
Persistence of plasma cells in the kidneys of autoimmune NZB/W mice (2001)
Cassese, G, Lindenau, S, De Boer, B, Arce, S, Hauser, A, Riemekasten, G, ...
No abstract available.
In vivo preactivated autoreactive Th cells in healthy individuals (2001)
Radbruch, A, Nitsch, S, Holzknecht, B, Gromnica-Ihle, E, Schneider, S, Hiepe, F, ...
No abstract available.
Yin, Z., Siegert, S., Neure, L., Grolms, M., Liu, L., Eggens, U., ...
Objectives.To quantify the T-helper type (Th) 1 cytokine interferon gamma (IFN-γ)-positive and the Th2 cytokine interleukin (IL)-4-positive cells in synovial fluid (SF) and synovial membrane (SM) at...
Donaldson, Louise E., Schmitt, Edgar, Huntley, John F., Newlands, George F. J., Grencis, Richard K., Radbruch, A
In common with many intestinal nematode infections, Trichinella spiralls infections in mice are associated with a pronounced intestinal mast cell hyperplasia. The expulsion of the parasite from the...
Induction of Th2 responses to soluble proteins is independent of B cell tolerance status (1995)
Van Mechelen, Marcelle, De Wit, Dominique, Ryelandt, Marion, Hjulstrom, Susanna, Heynderickx, Mirabel, Bazin, Hervé, ...
Injection of high doses of monomeric human gamma globulins (dHGG) In naive, adult mice causes antigen-specific tolerance of B cell and Th1 lymphocytes, while inducing the selective expansion of...
Up-regulation of monocytic IL-10 by tumor necrosis factor-{alpha} and cAMP elevating drugs (1995)
Platzer, C., Meisel, Ch., Vogt, K., Platzer, M., Radbruch, A.
It is well established that endotoxin [lipopolysacharide (LPS)] induces pro-inflammatory cytokine production in monocytes, which is followed by secretion of the anti-inflammatory cytokine, IL-10....
Huels, Christoph, Germann, Tieno, Goedert, Sigrid, Hoehn, Petra, Koelsch, Stephan, Hültner, Lothar, ...
Activation of nalve dense CD4+ T cells by plate-bound anti-CD3 antibodies favors the development of Th1 cells which, upon re-stimulation, produce significant amounts of INF-γ but no IL-4....
Sudowe, Stephan, Specht, Christoph, Kolbe, Ludger, Kölsch, Eckehart, Radbruch, A.
Priming of CBA/J mice with different doses of antigen has a profound effect on the ratio of IgE versus IgG antibodies appearing upon Immunization. Repeated injections of minute doses induce IgG...
Peter, Marcus E., Dhein, Jens, Ehret, Andreas, Hellbardt, Stefan, Walczak, Henning, Moldenhauer, Gerhard, ...
Certain B and T cell lines respond to activation signals, e.g. through the antigen receptor, by undergoing apoptotlc cell death. In T cells it has been recently shown that TCR-mediated apoptosis...
Goodman, David J., Gaff, Clara, Gerondakis, Steve, Radbruch, A.
Cytokine induced germline immunoglobulin heavy chain gene transcription appears to signal commitment to an isotype switch and may be the mechanism by which specific switch regions are targeted as the...
Kroese, Frans G. M., Ammerlaan, Willem A. M., Kantor, Aaron B., Radbruch, A.
B6-Sp6 transgenic mice carry fully rearranged (BALB/c-derived, Igh-Ca allotype) μ. heavy chain and ϰ light chain transgenes, specific for trinitrophenyl, on a C57BL background (Igh-Cb...
Frosch, Stefanie, Bonifas, Ursula, Reske-Kunz, Angelika B., Radbruch, A.
The antigen presentation capacity of bone marrow-derived macrophages (BMMph) was shown previously to be increased after stimulation with the lymphokines IFN-γ or granulocyte macrophage colony...
Violation of allelic exclusion of the T cell receptor {beta} genes in a helper T cell clone (1989)
Schittek, Birgit, Unkelbach, Elvira, Rajewsky, Klaus, Radbruch, A
The 4-hydroxy-3-nltrophenylacetyl coupled to chicken gamma globulin specific and I-Ab restricted helper T cell clone IH4 carries a Vβ8 1–Dβ2.1–Jβ2.3-rearrangement on one and a...
Whitmore, Alan C., Prowse, David M., Arnold, Larry W., Haughton, Geoffrey, Radbruch, A.
We have developed a method for enumerating the frequency of Ig isotype switching in clones of B cells. The method adapts Poisson statistics to analyze the distribution of amounts of switched isotype...
Manz, R, Assenmacher, M, Pflüger, E, Miltenyi, S, Radbruch, A
We have developed a technology for analysis and sorting of live cells according to secreted molecules. An artificial affinity matrix, specific for the secreted product of interest, is created on the...
Kochanek, S, Radbruch, A, Tesch, H, Renz, D, Doerfler, W
The genomic sequencing technique has been applied to assess the state of methylation in the DNA from human leukocyte subpopulations from healthy individuals and in the DNA from several individuals...
Evidence for transient requirement of the IgH enhancer.
Klein, S, Gerster, T, Picard, D, Radbruch, A, Schaffner, W
A transcriptional enhancer is thought to play a major role in determining tissue-specific expression of the immunoglobulin heavy chain (IgH) gene (1-3). However in three B-lymphoid cell lines the Ig...
Radbruch, A, Müller, W, Rajewsky, K
Mouse B lymphocytes can be activated polyclonally by bacterial lipopolysaccharide (LPS) to secrete Ig and perform Ig class switch. In the presence of the T-cell lymphokine B-cell differentiation...
Isolation of variants of mouse myeloma X63 that express changed immunoglobulin class.
Radbruch, A, Liesegang, B, Rajewsky, K
We have used fluorescence-activated cell sorting with class-specific antisera to isolate spontaneous variants in the expression of immunoglobulin heavy chain class from the mouse myeloma cell line...
Recombination between antibody heavy chain variable-region genes: evidence for gene conversion.
Krawinkel, U, Zoebelein, G, Brüggemann, M, Radbruch, A, Rajewsky, K
The murine hybridoma line B1-8.delta 1 secretes monoclonal IgD lambda 1 antibodies specific for the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP). The variable (V) region of these antibodies is defined...
Steplewski, Z, Spira, G, Blaszczyk, M, Lubeck, M D, Radbruch, A, Illges, H, ...
Three different monoclonal antibody isotypes, IgG1, IgG2b, and IgG2a, were derived by selection of isotype-switch variants from the CO-19-9 hybridoma. All three antibodies retained their binding...
Isolation of myeloma variants with predefined variant surface immunoglobulin by cell sorting.
Liesegang, B, Radbruch, A, Rajewsky, K
We describe a procedure for the isolation of somatic cell variants in which gene products are expressed on the cell surface that are not expressed in the wild type. Cloned cells of the myeloma line...
The distribution of immunoglobulin isotypes in activated B lymphocytes can be modulated by interleukin 4 (IL4), which enhances IgG1 and suppresses IgG3. We show here that IL4 induces transcription of...
A novel 34-kd protein co-isolated with the IgM molecule in surface IgM-expressing cells.
Hombach, J, Leclercq, L, Radbruch, A, Rajewsky, K, Reth, M
Plasmacytoma cells, transfected with a vector encoding a membrane-bound IgM molecule, do not show cell surface IgM expression, although complete IgM molecules are assembled intracellularly. The...
Brüggemann, M, Radbruch, A, Rajewsky, K
The hybridoma cell line B1-8.delta 1 expresses an IgD antibody with specificity for the hapten 4-hydroxy-3-nitro-phenylacetyl (NP). Two monoclonal antibodies, each recognizing an idiotypic...
Immunoglobulin V region variants in hybridoma cells. II. Recombination between V genes.
Dildrop, R, Brüggemann, M, Radbruch, A, Rajewsky, K, Beyreuther, K
The mouse hybridoma line B1-8.delta 1 secretes a monoclonal IgD, lambda 1 anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibody with defined idiotypic determinants. Two spontaneous V-region variants...
Directed Ig class switch recombination in activated murine B cells.
Winter, E, Krawinkel, U, Radbruch, A
Immunoglobulin class switch recombination occurs at frequencies of up to 10%/cell/generation in activated murine B-lymphocytes. We analysed cH gene rearrangements and switch recombinations from...
Yoshida, N, Radbruch, A, Rajewsky, K
In cultures of murine bone marrow cells colonies of 10(3)-10(4) cells were identified which consisted to a large part of pre-B and B cells. Cell mixing experiments with genetically marked cells...
Members of novel VH gene families are found in VDJ regions of polyclonally activated B-lymphocytes.
Winter, E, Radbruch, A, Krawinkel, U
Four potentially productive and two non-productive VDJ gene segments were isolated from the DNA of mouse B-lymphocytes which had been polyclonally activated by bacterial lipopolysaccharide (LPS)....
Deletion of Cmu genes in mouse B lymphocytes upon stimulation with LPS.
Mouse B lymphocytes can be activated polyclonally by bacterial lipopolysaccharide (LPS) to differentiate into plasmablasts. Within several days many cells perform immunoglobulin (Ig) class switching...
Klein, S, Sablitzky, F, Radbruch, A
Immunoglobulin (Ig) gene promotors are active only in cells of the B-lymphocyte lineage. Transfection experiments have shown that this is due in part to tissue specific 'activating' DNA sequences, so...
Wienands, J, Hombach, J, Radbruch, A, Riesterer, C, Reth, M
Two classes of immunoglobulin, IgM and IgD, are present as antigen receptors on the surface of mature B lymphocytes. We show here that IgD molecules are noncovalently associated in the B cell...
Manz, R, Assenmacher, M, Pflüger, E, Miltenyi, S, Radbruch, A
We have developed a technology for analysis and sorting of live cells according to secreted molecules. An artificial affinity matrix, specific for the secreted product of interest, is created on the...
Kochanek, S, Radbruch, A, Tesch, H, Renz, D, Doerfler, W
The genomic sequencing technique has been applied to assess the state of methylation in the DNA from human leukocyte subpopulations from healthy individuals and in the DNA from several individuals...
Evidence for transient requirement of the IgH enhancer.
Klein, S, Gerster, T, Picard, D, Radbruch, A, Schaffner, W
A transcriptional enhancer is thought to play a major role in determining tissue-specific expression of the immunoglobulin heavy chain (IgH) gene (1-3). However in three B-lymphoid cell lines the Ig...
Radbruch, A, Müller, W, Rajewsky, K
Mouse B lymphocytes can be activated polyclonally by bacterial lipopolysaccharide (LPS) to secrete Ig and perform Ig class switch. In the presence of the T-cell lymphokine B-cell differentiation...
Isolation of variants of mouse myeloma X63 that express changed immunoglobulin class.
Radbruch, A, Liesegang, B, Rajewsky, K
We have used fluorescence-activated cell sorting with class-specific antisera to isolate spontaneous variants in the expression of immunoglobulin heavy chain class from the mouse myeloma cell line...
Recombination between antibody heavy chain variable-region genes: evidence for gene conversion.
Krawinkel, U, Zoebelein, G, Brüggemann, M, Radbruch, A, Rajewsky, K
The murine hybridoma line B1-8.delta 1 secretes monoclonal IgD lambda 1 antibodies specific for the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP). The variable (V) region of these antibodies is defined...
Steplewski, Z, Spira, G, Blaszczyk, M, Lubeck, M D, Radbruch, A, Illges, H, ...
Three different monoclonal antibody isotypes, IgG1, IgG2b, and IgG2a, were derived by selection of isotype-switch variants from the CO-19-9 hybridoma. All three antibodies retained their binding...
Isolation of myeloma variants with predefined variant surface immunoglobulin by cell sorting.
Liesegang, B, Radbruch, A, Rajewsky, K
We describe a procedure for the isolation of somatic cell variants in which gene products are expressed on the cell surface that are not expressed in the wild type. Cloned cells of the myeloma line...
The distribution of immunoglobulin isotypes in activated B lymphocytes can be modulated by interleukin 4 (IL4), which enhances IgG1 and suppresses IgG3. We show here that IL4 induces transcription of...
A novel 34-kd protein co-isolated with the IgM molecule in surface IgM-expressing cells.
Hombach, J, Leclercq, L, Radbruch, A, Rajewsky, K, Reth, M
Plasmacytoma cells, transfected with a vector encoding a membrane-bound IgM molecule, do not show cell surface IgM expression, although complete IgM molecules are assembled intracellularly. The...
Wienands, J, Hombach, J, Radbruch, A, Riesterer, C, Reth, M
Two classes of immunoglobulin, IgM and IgD, are present as antigen receptors on the surface of mature B lymphocytes. We show here that IgD molecules are noncovalently associated in the B cell...
Brüggemann, M, Radbruch, A, Rajewsky, K
The hybridoma cell line B1-8.delta 1 expresses an IgD antibody with specificity for the hapten 4-hydroxy-3-nitro-phenylacetyl (NP). Two monoclonal antibodies, each recognizing an idiotypic...
Immunoglobulin V region variants in hybridoma cells. II. Recombination between V genes.
Dildrop, R, Brüggemann, M, Radbruch, A, Rajewsky, K, Beyreuther, K
The mouse hybridoma line B1-8.delta 1 secretes a monoclonal IgD, lambda 1 anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibody with defined idiotypic determinants. Two spontaneous V-region variants...
Directed Ig class switch recombination in activated murine B cells.
Winter, E, Krawinkel, U, Radbruch, A
Immunoglobulin class switch recombination occurs at frequencies of up to 10%/cell/generation in activated murine B-lymphocytes. We analysed cH gene rearrangements and switch recombinations from...
Yoshida, N, Radbruch, A, Rajewsky, K
In cultures of murine bone marrow cells colonies of 10(3)-10(4) cells were identified which consisted to a large part of pre-B and B cells. Cell mixing experiments with genetically marked cells...
Members of novel VH gene families are found in VDJ regions of polyclonally activated B-lymphocytes.
Winter, E, Radbruch, A, Krawinkel, U
Four potentially productive and two non-productive VDJ gene segments were isolated from the DNA of mouse B-lymphocytes which had been polyclonally activated by bacterial lipopolysaccharide (LPS)....
Deletion of Cmu genes in mouse B lymphocytes upon stimulation with LPS.
Mouse B lymphocytes can be activated polyclonally by bacterial lipopolysaccharide (LPS) to differentiate into plasmablasts. Within several days many cells perform immunoglobulin (Ig) class switching...
Klein, S, Sablitzky, F, Radbruch, A
Immunoglobulin (Ig) gene promotors are active only in cells of the B-lymphocyte lineage. Transfection experiments have shown that this is due in part to tissue specific 'activating' DNA sequences, so...
Rudwaleit, M., Yin, Z., Siegert, S., Grolms, M., Radbruch, A., Braun, J., ...
OBJECTIVE—This study was performed to assess whether there is any change in the T cell cytokine pattern in early rheumatoid arthritis (RA) patients treated with methotrexate (MTX) and whether the...
Rudwaleit, M, Siegert, S, Yin, Z, Eick, J, Thiel, A, Radbruch, A, ...
OBJECTIVE—To test the hypothesis that ankylosing spondylitis (AS) is a T helper cell type 2 polarised disease by quantifying the T cell cytokines interferon γ (IFNγ), interleukin 4 (IL4),...
Perturbations of peripheral B lymphocyte homoeostasis in children with systemic lupus erythematosus
Odendahl, M, Keitzer, R, Wahn, U, Hiepe, F, Radbruch, A, Dorner, T, ...
Objective: To investigate the distribution of peripheral B cell subpopulations of children with active and inactive systemic lupus erythematosus (SLE) compared with healthy controls.
Braun, J., Xiang, J., Brandt, J., Maetzel, H., Haibel, H., Wu, P., ...
HORST, A, HUNZELMANN, N, ARCE, S, HERBER, M, MANZ, R A, RADBRUCH, A, ...
In atopic patients and patients with hyper-IgE syndrome (HIE) highly elevated IgE serum levels can be detected. Due to their very low frequency little is known about IgE-producing plasma cells (PC)...
Cell therapy for autoimmune diseases: does it have a future?
Almost all current therapeutic concepts in autoimmune diseases are based on the systemic suppression of immune functions and are not curative. The recent introduction of biologicals such as tumour...