Mutation detection using a novel plant endonuclease.
Oleykowski, C A, Bronson Mullins, C R, Godwin, A K, Yeung, A T
We have discovered a useful new reagent for mutation detection, a novel nuclease CEL I from celery. It is specific for DNA distortions and mismatches from pH 6 to 9. Incision is on the 3'-side of the...
Yeung, A T, Bascomb, N F, Turner, K J, Schmidt, R R
By use of a rocket immunoelectrophoresis-activity stain procedure, it was shown that catalytic activity of an ammonium-inducible nicotinamide adenine dinucleotide phosphate-specific glutamate...
Bascomb, N F, Yeung, A T, Turner, K J, Schmidt, R R
When ammonia was removed from Chlorella sorokiniana cells, which contain an ammonium-inducible nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase (NADP-GDH), the activity of...
Gronostajski, R M, Yeung, A T, Schmidt, R R
The nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase (NADP-GDH) of Chlorella sorokiniana was purified 260-fold to electrophoretic homogeneity in six steps. Depending on...
Israel, D W, Gronostajski, R M, Yeung, A T, Schmidt, R R
Earlier studies indicated that the gene of an ammonium-inducible glutamate dehydrogenase (GDH) was inducible throughout the cell cycle and was expressible shortly after replication early in the...
Using an oligonucleotide model substrate, we observed two unusual mechanisms of UvrABC endonuclease in the repair of 4,5',8-trimethylpsoralen monoadducts and crosslinks. (i) UvrABC endonuclease...
Lambert, B, Jones, B K, Roques, B P, Le Pecq, J B, Yeung, A T
We have demonstrated that the noncovalent complex formed between DNA and an antitumor bifunctional intercalator, ditercalinium, is recognized in vitro as bulky covalent DNA lesions by the purified...
The repair of psoralen monoadducts by the Escherichia coli UvrABC endonuclease.
Yeung, A T, Jones, B K, Capraro, M, Chu, T
We have examined the interactions of UvrABC endonuclease with DNA containing the monoadducts of 8-methoxypsoralen (8-MOP) and 4,5',8-trimethylpsoralen (TMP). The UvrA and UvrB proteins were found to...
Hang, B, Yeung, A T, Lambert, M W
A DNA binding protein with specificity for DNA containing interstrand cross-links induced by 4,5',8-trimethylpsoralen (TMP) plus long wavelength ultraviolet (UVA) light has been identified in normal...
The purification of the Escherichia coli UvrABC incision system.
Yeung, A T, Mattes, W B, Oh, E Y, Yoakum, G H, Grossman, L
The UvrA, UvrB and UvrC proteins of Escherichia coli have been purified in good yields to homogeneity with rapid three- or four-step purification procedures. The cloned uvrA and uvrB genes were...
Modifications of guanine bases during oligonucleotide synthesis.
Yeung, A T, Dinehart, W J, Jones, B K
Guanine bases are sensitive to modification during automated DNA synthesis and processing reactions. Methods for the detection of two types of guanine modifications are described. The first method...
Yeung, A T, Mattes, W B, Grossman, L
An examination has been made into the nature of the nucleoprotein complexes formed during the incision reaction catalyzed by the Escherichia coli UvrABC endonuclease when acting on a pyrimidine...
Yoakum, G H, Yeung, A T, Mattes, W B, Grossman, L
We have constructed a hybrid pKC30-uvrA plasmid (pGHY5003) in which transcription of the uvrA gene can be induced under pL control to amplify the uvrA gene product to 7% of cellular protein. To...
Enzymatic properties of purified Escherichia coli uvrABC proteins.
Yeung, A T, Mattes, W B, Oh, E Y, Grossman, L
The cloned uvrA and uvrB genes of Escherichia coli K-12 were amplified by linkage to the PL promoter of plasmid pKC30. The uvrC gene was amplified in the high-copy-number plasmid pRLM 24. The three...
High-Throughput Screening for Induced Point Mutations
Colbert, T., Till, B. J., Tompa, R., Reynolds, S., Steine, M. N., Yeung, A. T., ...
Mutation detection using a novel plant endonuclease.
Oleykowski, C A, Bronson Mullins, C R, Godwin, A K, Yeung, A T
We have discovered a useful new reagent for mutation detection, a novel nuclease CEL I from celery. It is specific for DNA distortions and mismatches from pH 6 to 9. Incision is on the 3'-side of the...
Yeung, A T, Bascomb, N F, Turner, K J, Schmidt, R R
By use of a rocket immunoelectrophoresis-activity stain procedure, it was shown that catalytic activity of an ammonium-inducible nicotinamide adenine dinucleotide phosphate-specific glutamate...
Bascomb, N F, Yeung, A T, Turner, K J, Schmidt, R R
When ammonia was removed from Chlorella sorokiniana cells, which contain an ammonium-inducible nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase (NADP-GDH), the activity of...
Gronostajski, R M, Yeung, A T, Schmidt, R R
The nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase (NADP-GDH) of Chlorella sorokiniana was purified 260-fold to electrophoretic homogeneity in six steps. Depending on...
Israel, D W, Gronostajski, R M, Yeung, A T, Schmidt, R R
Earlier studies indicated that the gene of an ammonium-inducible glutamate dehydrogenase (GDH) was inducible throughout the cell cycle and was expressible shortly after replication early in the...
Using an oligonucleotide model substrate, we observed two unusual mechanisms of UvrABC endonuclease in the repair of 4,5',8-trimethylpsoralen monoadducts and crosslinks. (i) UvrABC endonuclease...
Lambert, B, Jones, B K, Roques, B P, Le Pecq, J B, Yeung, A T
We have demonstrated that the noncovalent complex formed between DNA and an antitumor bifunctional intercalator, ditercalinium, is recognized in vitro as bulky covalent DNA lesions by the purified...
The repair of psoralen monoadducts by the Escherichia coli UvrABC endonuclease.
Yeung, A T, Jones, B K, Capraro, M, Chu, T
We have examined the interactions of UvrABC endonuclease with DNA containing the monoadducts of 8-methoxypsoralen (8-MOP) and 4,5',8-trimethylpsoralen (TMP). The UvrA and UvrB proteins were found to...
Hang, B, Yeung, A T, Lambert, M W
A DNA binding protein with specificity for DNA containing interstrand cross-links induced by 4,5',8-trimethylpsoralen (TMP) plus long wavelength ultraviolet (UVA) light has been identified in normal...
The purification of the Escherichia coli UvrABC incision system.
Yeung, A T, Mattes, W B, Oh, E Y, Yoakum, G H, Grossman, L
The UvrA, UvrB and UvrC proteins of Escherichia coli have been purified in good yields to homogeneity with rapid three- or four-step purification procedures. The cloned uvrA and uvrB genes were...
Modifications of guanine bases during oligonucleotide synthesis.
Yeung, A T, Dinehart, W J, Jones, B K
Guanine bases are sensitive to modification during automated DNA synthesis and processing reactions. Methods for the detection of two types of guanine modifications are described. The first method...
Yeung, A T, Mattes, W B, Grossman, L
An examination has been made into the nature of the nucleoprotein complexes formed during the incision reaction catalyzed by the Escherichia coli UvrABC endonuclease when acting on a pyrimidine...
Yoakum, G H, Yeung, A T, Mattes, W B, Grossman, L
We have constructed a hybrid pKC30-uvrA plasmid (pGHY5003) in which transcription of the uvrA gene can be induced under pL control to amplify the uvrA gene product to 7% of cellular protein. To...
Enzymatic properties of purified Escherichia coli uvrABC proteins.
Yeung, A T, Mattes, W B, Oh, E Y, Grossman, L
The cloned uvrA and uvrB genes of Escherichia coli K-12 were amplified by linkage to the PL promoter of plasmid pKC30. The uvrC gene was amplified in the high-copy-number plasmid pRLM 24. The three...