Merson, M.H., Sack, R.B., Islam, S., Saklayen, G., Huda, N., Huq, I., ...
The clinical characteristics of disease due to enterotoxigenic Escherichia coli (ETEC) were determined in 88 adult males admitted to a hospital in Dacca, Bangladesh, with moderate to severe...
Seroepidemiology of rotavirus infection in rural Bangladesh (1980)
Sack, David A., Gilman, Robert H., Kapikian, A.Z., Aziz, K.M.S.
A prospective seroepidemiological study of rotavirus infection was performed in children in a village in rural Bangladesh. Ninety-three percent of the children had detectable antibodies during the...
Antigenic relationships among human rotaviruses as determined by outer capsid protein VP4.
Gorziglia, M, Larralde, G, Kapikian, A Z, Chanock, R M
cDNA clones representing the VP4 gene of symptomatic human rotavirus strain KU (VP7 serotype 1) or DS-1 (VP7 serotype 2) or asymptomatic human rotavirus strain 1076 (VP7 serotype 2) were constructed...
Detection and Genotyping of Human Group A Rotaviruses by Oligonucleotide Microarray Hybridization
Chizhikov, V., Wagner, M., Ivshina, A., Hoshino, Y., Kapikian, A. Z., Chumakov, K.
A rapid and reliable method for the identification of five clinically relevant G genotypes (G1 to G4 and G9) of human rotaviruses based on oligonucleotide microarray hybridization has been developed....
Greenberg, H B, Valdesuso, J R, Kalica, A R, Wyatt, R G, McAuliffe, V J, Kapikian, A Z, ...
The proteins of the Norwalk virus were studied by polyacrylamide gel electrophoresis. Highly purified specifically immunoprecipitated virions appeared to contain a single primary structural protein...
Rotavirus vaccines: an overview.
Rotavirus vaccine development has focused on the delivery of live attenuated rotavirus strains by the oral route. The initial "Jennerian" approach involving bovine (RIT4237, WC3) or rhesus (RRV)...
Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease.
McIntosh, K, Dees, J H, Becker, W B, Kapikian, A Z, Chanock, R M
Ward, R L, Kapikian, A Z, Goldberg, K M, Knowlton, D R, Watson, M W, Rappaport, R
Comparisons in rotavirus neutralizing-antibody responses were made with sera collected from vaccinated infants. The methods were a plaque reduction assay and a new enzyme-linked immunosorbent...
Green, K Y, Kapikian, A Z, Valdesuso, J, Sosnovtsev, S, Treanor, J J, Lew, J F
The Norwalk and Hawaii viruses are antigenically distinct members of the family Caliciviridae and are considered to be important etiologic agents of epidemic gastroenteritis, with most studies...
Identification of minireovirus as a Norwalk-like virus in pediatric patients with gastroenteritis.
Lew, J F, Petric, M, Kapikian, A Z, Jiang, X, Estes, M K, Green, K Y
In 1977, 30- to 32-nm virus-like particles, named minireovirus because of their unique morphologic appearance, were detected by electron microscopy in the stools of infants and young children with...
Hoshino, Y, Kapikian, A Z, Chanock, R M
Group A human rotavirus strains D, Wa, DS-1, and P were originally recovered from children with diarrhea. In an attempt to attenuate virulent, wild-type human rotaviruses of major epidemiological...
Sera from 17 of 18 adult volunteers challenged with a virulent serotype 1 rotavirus strain (D) were examined for prechallenge antibody levels against several well-defined rotavirus VP7 and VP4...
Serotype-specific epitope(s) present on the VP8 subunit of rotavirus VP4 protein.
Larralde, G, Li, B G, Kapikian, A Z, Gorziglia, M
cDNA clones representing the VP8 and VP5 subunits of VP4 of symptomatic human rotavirus strain KU (VP7 serotype 1 and VP4 serotype 1A) or DS-1 (VP7 serotype 2 and VP4 serotype 1B) or asymptomatic...
Expression of the OSU rotavirus outer capsid protein VP4 by an adenovirus recombinant.
Full-length cDNA of the VP4 gene of porcine rotavirus strain OSU was cloned into adenovirus type 5 (Ad5) downstream of the E3 promoter. The plaque-purified recombinant (Ad5-OSU VP4) expressed...
Flores, J, Sears, J, Schael, I P, White, L, Garcia, D, Lanata, C, ...
We have synthesized 32P-labeled hybridization probes from a hyperdivergent region (nucleotides 51 to 392) of the rotavirus gene encoding the VP7 glycoprotein by using the polymerase chain reaction...
Taniguchi, K, Nishikawa, K, Urasawa, T, Urasawa, S, Midthun, K, Kapikian, A Z, ...
In our previous study (K. Taniguchi, Y. Morita, T. Urasawa, and S. Urasawa, J. Virol. 62:2421-2426, 1987) in which the cross-reactive neutralization epitopes on VP4 of human rotaviruses were...
Prediction of human rotavirus serotype by nucleotide sequence analysis of the VP7 protein gene.
Green, K Y, Sears, J F, Taniguchi, K, Midthun, K, Hoshino, Y, Gorziglia, M, ...
Human rotavirus field isolates were characterized by direct sequence analysis of the gene encoding the serotype-specific major neutralization protein (VP7). Single-stranded RNA transcripts were...
Taniguchi, K, Hoshino, Y, Nishikawa, K, Green, K Y, Maloy, W L, Morita, Y, ...
The neutralization epitopes of human and simian rotavirus protein VP7 were studied by producing six neutralizing monoclonal antibodies (N-MAbs) and using these N-MAbs to select antigenic mutants that...
Flores, J, Midthun, K, Hoshino, Y, Green, K, Gorziglia, M, Kapikian, A Z, ...
RNA-RNA hybridization was performed to assess the extent of genetic relatedness among human rotaviruses isolated from children with gastroenteritis and from asymptomatic newborn infants. 32P-labeled...
Taniguchi, K, Maloy, W L, Nishikawa, K, Green, K Y, Hoshino, Y, Urasawa, S, ...
The group A rotaviruses are composed of at least seven serotypes. Serotype specificity is defined mainly by an outer capsid protein, VP7. In contrast, the other surface protein, VP3 (775 amino...
Genetic stability of rotaviruses recovered from asymptomatic neonatal infections.
Flores, J, Sears, J, Green, K Y, Perez-Schael, I, Morantes, A, Daoud, G, ...
The sequence of the VP7 gene from 19 rotavirus strains recovered from asymptomatically infected newborn infants was determined by direct analysis of transcript RNAs synthesized from virus present in...
Hoshino, Y, Saif, L J, Sereno, M M, Chanock, R M, Kapikian, A Z
A single-gene substitution reassortant 11-1 was generated from two porcine rotaviruses, OSU (serotype 5) and Gottfried (serotype 4). This reassortant derived 10 genes, including gene 4 encoding VP3,...
Gorziglia, M, Green, K, Nishikawa, K, Taniguchi, K, Jones, R, Kapikian, A Z, ...
The complete nucleotide sequence of the fourth gene of symptomatic (Wa, DS-1, P, and VA70) and asymptomatic (M37, 1076, McN13, and ST3) rotaviruses of serotype 1, 2, 3, or 4 was determined by the...
Nishikawa, K, Taniguchi, K, Torres, A, Hoshino, Y, Green, K, Kapikian, A Z, ...
The gene encoding outer capsid protein VP3 of subpopulations of two animal rotaviruses, simian SA11 and Nebraska calf diarrhea virus (NCDV), was analyzed. Two laboratory strains of simian SA11...
Reassortant rotaviruses as potential live rotavirus vaccine candidates.
Midthun, K, Greenberg, H B, Hoshino, Y, Kapikian, A Z, Wyatt, R G, Chanock, R M
A series of reassortants was isolated from coinfection of cell cultures with a wild-type animal rotavirus and a "noncultivatable" human rotavirus. Wild-type bovine rotavirus (UK strain) was...
In vitro transcription of two human rotaviruses.
Flores, J, Myslinski, J, Kalica, A R, Greenberg, H B, Wyatt, R G, Kapikian, A Z, ...
The RNA polymerase activities of a cultivatable (Wa) and a noncultivatable (DS-1) strain of human rotavirus were studied. Under optimal conditions, transcription of all of their RNA segments...
Midthun, K, Greenberg, H B, Kurtz, J B, Gary, G W, Lin, F Y, Kapikian, A Z
The Marin County strain of type 5 astrovirus was associated with two separate outbreaks of nonbacterial gastroenteritis in California in 1978. A safety-tested, bacterium-free filtrate prepared from a...
Monoclonal antibodies to subgroup 1 rotavirus.
Singh, N, Sereno, M M, Flores, J, Kapikian, A Z
Subgroup 1-specific monoclones were analyzed and used successfully in an enzyme-linked immunosorbent assay to recognize certain subgroup 1 rotaviruses.
Isolation, propagation, and characterization of a second equine rotavirus serotype.
Hoshino, Y, Wyatt, R G, Greenberg, H B, Kalica, A R, Flores, J, Kapikian, A Z
A rotavirus designated strain H-2 was isolated in primary African green monkey kidney cells from a foal with diarrhea. This cell culture-adapted strain was found to be similar, if not identical, to...
Hoshino, Y, Wyatt, R G, Greenberg, H B, Kalica, A R, Flores, J, Kapikian, A Z
By the plaque reduction neutralization test, the CU-1 strain of canine rotavirus was similar, if not identical, to three strains (no. 14, no. 15, and P) of the tentatively designated third human...
Serotype analysis of rotaviruses from different locations in Malaysia.
Rasool, N B, Green, K Y, Kapikian, A Z
The distribution of rotavirus G (VP7) serotypes circulating in four locations in Malaysia, representing three geographical areas, was evaluated in 341 RNA-positive stool specimens obtained...
Green, K Y, Lew, J F, Jiang, X, Kapikian, A Z, Estes, M K
Since the discovery of the Norwalk virus (NV) by immune electron microscopy (IEM) in 1972, serologic studies with this virus have relied on particle-positive fecal material from infected volunteers...
Flores, J, Perez-Schael, I, Blanco, M, Rojas, A M, Alfonzo, E, Crespo, I, ...
We evaluated the reactogenicity and antigenicity of a quadrivalent rotavirus vaccine composed of serotype 3 rhesus rotavirus (RRV) and three single-gene-substitution reassortants of RRV and human...
Hoshino, Y, Sereno, M M, Midthun, K, Flores, J, Chanock, R M, Kapikian, A Z
The SB-1A rotavirus recovered from a diarrheic piglet in the United States is a naturally occurring intertypic rotavirus. When studied by reciprocal neutralization tests, the SB-1A virus was similar,...
Midthun, K, Valdesuso, J, Hoshino, Y, Flores, J, Kapikian, A Z, Chanock, R M
Antigenic characterization of human and animal rotaviruses by the plaque reduction neutralization assay has shown the existence of naturally occurring intertypes. Antiserum to M37, a rotavirus strain...
Flores, J, Taniguchi, K, Green, K, Perez-Schael, I, Garcia, D, Sears, J, ...
We have used a recently developed monoclonal antibody enzyme-linked immunosorbent assay (K. Taniguchi, T. Urasawa, Y. Morita, H. B. Greenberg, and S. Urasawa, J. Infect. Dis. 155:1159-1166, 1987) for...
Midthun, K, Pang, L Z, Flores, J, Kapikian, A Z
In a phase 1 study to evaluate human-rhesus rotavirus reassortant vaccines, 116 infants 1 to 5 months of age received one of the following five preparations: the serotype 1 reassortant, the serotype...
Dot hybridization assay for distinction of rotavirus serotypes.
Flores, J, Green, K Y, Garcia, D, Sears, J, Perez-Schael, I, Avendaño, L F, ...
We have developed a hybridization assay that permits distinction of rotavirus serotypes 1, 2, 3, and 4. The serotype of rotaviruses from stool samples or tissue culture was recognized by...
Flores, J, Perez-Schael, I, Blanco, M, Vilar, M, Garcia, D, Perez, M, ...
The reactions to and antigenicity of two human-rhesus rotavirus (RRV) reassortants (human rotavirus strain D x RRV and human rotavirus strain DS1 x RRV) with the VP7 neutralization specificity of a...
Midthun, K, Valdesuso, J, Kapikian, A Z, Hoshino, Y, Green, K Y
Hybridomas producing monoclonal antibodies to VP7, a major neutralizing protein of serotype 9 rotavirus (strain W161), were prepared. One monoclonal antibody, W161-6A1, was shown to neutralize only...
Midthun, K, Hoshino, Y, Kapikian, A Z, Chanock, R M
A series of reassortants was isolated from coinfection of cell cultures with wild-type bovine rotavirus (UK strain [serotype 6]) or rhesus rotavirus (strain MMU18006 [serotype 3]) and a tissue...
Nakagomi, O, Nakagomi, T, Hoshino, Y, Flores, J, Kapikian, A Z
We have previously found (O. Nakagomi, T. Nakagomi, H. Oyamada, and T. Suto, J. Med. Virol. 17:29-34, 1985), during an epidemiological study in Japan, a novel human rotavirus that belongs to subgroup...
Midthun, K, Flores, J, Taniguchi, K, Urasawa, S, Kapikian, A Z, Chanock, R M
Antigenic characterization of human rotaviruses by plaque reduction neutralization assay has revealed four distinct serotypes. The outer capsid protein VP7, coded for by gene 8 or 9, is a major...
Clinical studies of a quadrivalent rotavirus vaccine in Venezuelan infants.
Perez-Schael, I, Blanco, M, Vilar, M, Garcia, D, White, L, Gonzalez, R, ...
Phase I studies of an oral quadrivalent rotavirus vaccine were conducted in 130 Venezuelan infants 10 to 20 weeks of age. The vaccine consists of a mixture of equal amounts of rhesus rotavirus (RRV)...
Reactivities of serotyping monoclonal antibodies with culture-adapted human rotaviruses.
Ward, R L, McNeal, M M, Clemens, J D, Sack, D A, Rao, M, Huda, N, ...
Rotaviruses collected in Bangladesh during 1985 to 1986 were culture adapted and used in a comparative serotyping study with three groups of monoclonal antibodies, all of which reacted with the major...
Pediatric viral gastroenteritis during eight years of study.
Brandt, C D, Kim, H W, Rodriguez, W J, Arrobio, J O, Jeffries, B C, Stallings, E P, ...
During the period January 1974 through July 1982, fecal samples from 1,537 pediatric inpatients with gastroenteritis were tested for enteric viruses by electron microscopic and rotavirus...
Wyatt, R G, James, H D, Pittman, A L, Hoshino, Y, Greenberg, H B, Kalica, A R, ...
Of 73 rotavirus-positive fecal specimens tested, 39 yielded a human rotavirus that could be cultivated serially in MA104 or primary African green monkey kidney cells or both; 18 were serotyped. Four...
Wyatt, R G, Kapikian, A Z, Mebus, C A
Sera from calves infected in utero or postnatally with bovine rotavirus NCDV or postnatally with human rotavirus D (serotype 1) were tested by plaque reduction neutralization assay for antibody to...
Isolation and characterization of an equine rotavirus.
Hoshino, Y, Wyatt, R G, Greenberg, H B, Kalica, A R, Flores, J, Kapikian, A Z
A rotavirus, designated as the H-1 strain, was isolated from a diarrheic foal in primary African green monkey kidney cells and MA104 cells. This cell culture-adapted strain hemagglutinated...
Serotypic characterization of rotaviruses derived from asymptomatic human neonatal infections.
Hoshino, Y, Wyatt, R G, Flores, J, Midthun, K, Kapikian, A Z
Nineteen rotavirus strains derived from asymptomatic neonates (seven from England, five from Australia, two from Venezuela, and five from Sweden) were successfully cultivated in primary African green...
Isolation of human rotavirus subgroups 1 and 2 in cell culture.
Kutsuzawa, T, Konno, T, Suzuki, H, Kapikian, A Z, Ebina, T, Ishida, N
One strain of human rotavirus subgroup 1 (KUN) and one strain of subgroup 2 (MO) were isolated with the MA104 cell line, a fetal rhesus monkey kidney cell line. Their subgroup specificities and RNA...
Enhancement of antigen incorporation and infectivity of cell cultures by human rotavirus.
Schoub, B D, Kalica, A R, Greenberg, H B, Bertran, D M, Sereno, M M, Wyatt, R G, ...
Infection of cell cultures with human rotavirus preparations was attempted and the effects of trypsin and low-speed centrifugation on antigen incorporation, as demonstrated by immunofluorescence and...
Seroepidemiology of rotavirus infection in rural Bangladesh.
Sack, D A, Gilman, R H, Kapikian, A Z, Aziz, K M
A prospective seroepidemiological study of rotavirus infection was performed in children in a village in rural Bangladesh. Ninety-three percent of the children had detectable antibodies during the...
Brandt, C D, Kim, H W, Rodriguez, W J, Thomas, L, Yolken, R H, Arrobio, J O, ...
An approximate 10% suspension in water of the first available stool sample from 411 infants and young children with acute gastroenteritis was examined by electron microscopy (EM) after 2 min of...
Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.
Yolken, R H, Greenberg, H B, Merson, M H, Sack, R B, Kapikian, A Z
The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity...
Hemagglutination by simian rotavirus.
Kalica, A R, James, J D, Kapikian, A Z
A simian rotavirus (SA-11) was shown to hemagglutinate human group "O" and guinea pig erythrocytes. The hemagglutinin appeared to be associated with the outer capsid of the SA-11 virus and was...
Measurement of rotavirus antibody by an enzyme-linked immunosorbent assay blocking assay.
Yolken, R H, Wyatt, R G, Barbour, B A, Kim, H W, Kapikian, A Z, Chanock, R M
A new method for the measurement of rotavirus antibody is described, utilizing the system of enzyme-linked immunosorbent assay (ELISA). In this method, serum is incubated with a fixed amount of...
Greenberg, H B, Kalica, A R, Wyatt, R G, Jones, R W, Kapikian, A Z, Chanock, R M
Fastidious human rotaviruses that did not undergo productive infection in tissue culture were rescued by genetic reassortment during mixed infection with a temperature-sensitive (ts) mutant of a...
Rescue and serotypic characterization of noncultivable human rotavirus by gene reassortment.
Greenberg, H B, Wyatt, R G, Kapikian, A Z, Kalica, A R, Flores, J, Jones, R
Thirty-three of 50 noncultivable human rotavirus strains from a variety of locations were successfully rescued by gene reassortment. The serotype of each of the 33 strains was investigated by a...
Definition of human rotavirus serotypes by plaque reduction assay.
Wyatt, R G, Greenberg, H B, James, W D, Pittman, A L, Kalica, A R, Flores, J, ...
Twenty different human rotavirus reassortants were characterized serologically by a plaque reduction assay as belonging to one of three distinct serotypes. Fourteen were similar if not identical to...
Ryder, R W, Greenberg, H, Singh, N, Oro, G, De Guardia, A, Sack, R B, ...
Serum antibody titrations against the heat-labile enterotoxin (LT) of Escherichia coli were carried out on Panamanians, U.S. citizens resident in the Panama Canal Zone, Apache Indians living on the...