Abdelghani Sghir

Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian patients with reactive and undifferentiated arthritis (2009)

Siala, Mariam, Gdoura, Radhouane, Fourati, Hela, Rihl, Markus, Jaulhac, Benoit, Younes, Mohamed, ...

Abstract Introduction Broad-range rDNA PCR provides an alternative, cultivation-independent approach for identifying bacterial DNA in reactive and other form of arthritis. The aim of this study was...

Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing (2008)

Siala, Mariam, Jaulhac, Benoit, Gdoura, Radhouane, Sibilia, Jean, Fourati, Hela, Younes, Mohamed, ...

Abstract Introduction Bacteria and/or their antigens have been implicated in the pathogenesis of reactive arthritis (ReA). Several studies have reported the presence of bacterial antigens and nucleic...

Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization (2000)

Abdelghani Sghir, Genevieve Gramet, Antonia Suau, Violaine Rochet, Philippe Pochart, Joel Dore

To investigate the population structure of the predominant phylogenetic groups within the human adult fecal microbiota, a new oligonucleotide probe designated S-G-Clept-1240-a-A-18 was designed,...

Quantification of Bacterial Groups within Human Fecal Flora by Oligonucleotide Probe Hybridization

Sghir, Abdelghani, Gramet, Genevieve, Suau, Antonia, Rochet, Violaine, Pochart, Philippe, Dore, Joel

To investigate the population structure of the predominant phylogenetic groups within the human adult fecal microbiota, a new oligonucleotide probe designated S-G-Clept-1240-a-A-18 was designed,...

Molecular Evidence for Novel Planctomycete Diversity in a Municipal Wastewater Treatment Plant

Chouari, Rakia, Le Paslier, Denis, Daegelen, Patrick, Ginestet, Philippe, Weissenbach, Jean, Sghir, Abdelghani

We examined anoxic and aerobic basins and an anaerobic digestor of a municipal wastewater treatment plant for the presence of novel planctomycete-like diversity. Three 16S rRNA gene libraries were...

Novel Major Bacterial Candidate Division within a Municipal Anaerobic Sludge Digester

Chouari, Rakia, Le Paslier, Denis, Dauga, Catherine, Daegelen, Patrick, Weissenbach, Jean, Sghir, Abdelghani

In a previous study, we analyzed the molecular diversity of Planctomycetales by PCR amplification and sequencing of 16S rRNA clone libraries generated from a municipal wastewater plant, using...

Quantification of Bacterial Groups within Human Fecal Flora by Oligonucleotide Probe Hybridization

Sghir, Abdelghani, Gramet, Genevieve, Suau, Antonia, Rochet, Violaine, Pochart, Philippe, Dore, Joel

To investigate the population structure of the predominant phylogenetic groups within the human adult fecal microbiota, a new oligonucleotide probe designated S-G-Clept-1240-a-A-18 was designed,...

Molecular Evidence for Novel Planctomycete Diversity in a Municipal Wastewater Treatment Plant

Chouari, Rakia, Le Paslier, Denis, Daegelen, Patrick, Ginestet, Philippe, Weissenbach, Jean, Sghir, Abdelghani

We examined anoxic and aerobic basins and an anaerobic digestor of a municipal wastewater treatment plant for the presence of novel planctomycete-like diversity. Three 16S rRNA gene libraries were...

Novel Major Bacterial Candidate Division within a Municipal Anaerobic Sludge Digester

Chouari, Rakia, Le Paslier, Denis, Dauga, Catherine, Daegelen, Patrick, Weissenbach, Jean, Sghir, Abdelghani

In a previous study, we analyzed the molecular diversity of Planctomycetales by PCR amplification and sequencing of 16S rRNA clone libraries generated from a municipal wastewater plant, using...

“Candidatus Cloacamonas Acidaminovorans”: Genome Sequence Reconstruction Provides a First Glimpse of a New Bacterial Division▿ †

Pelletier, Eric, Kreimeyer, Annett, Bocs, Stéphanie, Rouy, Zoé, Gyapay, Gábor, Chouari, Rakia, ...

Many microorganisms live in anaerobic environments. Most of these microorganisms have not yet been cultivated. Here, we present, from a metagenomic analysis of an anaerobic digester of a municipal...

Discovery and characterization of a new bacterial candidate division by an anaerobic sludge digester metagenomic approach

Guermazi, Sonda, Daegelen, Patrick, Dauga, Catherine, Rivière, Delphine, Bouchez, Théodore, Godon, Jean Jacques, ...

We have constructed a large fosmid library from a mesophilic anaerobic digester and explored its 16S rDNA diversity using a high-density filter DNA–DNA hybridization procedure. We identified a...