A noncoding RNA gene on chromosome 10p15.3 may function upstream of hTERT (2009)
Miura, Norimasa, Sato, Reina, Tsukamoto, Tomoe, Shimizu, Mika, Kabashima, Hiroko, Takeda, Miho, ...
Abstract Background We attempted to clone candidate genes on 10p14–15 which may regulate hTERT expression, through exon trapping using 3 BAC clones covering the region. After obtaining 20 exons, we...
Predicting drug susceptibility of non-small cell lung cancers based on genetic lesions (2009)
Sos, Martin L., Michel, Kathrin, Zander, Thomas, Weiss, Jonathan, Frommolt, Peter, Peifer, Martin, ...
Sos, Martin, Koker, Mirjam, Weir, Barbara, Heynck, Stefanie, Rabinovsky, Rosalia, Zander, Thomas, ...
Sos, Martin L., Koker, Mirjam, Weir, Barbara A., Heynck, Stefanie, Rabinovsky, Rosalia, Zander, Thomas, ...
Predicting drug susceptibility of non-small cell lung cancers based on genetic lesions. (2009)
Sos, Martin L., Michel, Kathrin, Zander, Thomas, Weiss, Jonathan, Frommolt, Peter, Peifer, Martin, ...
Somatic genetic alterations in cancers have been linked with response to targeted therapeutics by creation of specific dependency on activated oncogenic signaling pathways. However, no tools...
Sos, Martin L., Koker, Mirjam, Weir, Barbara A., Heynck, Stefanie, Rabinovsky, Rosalia, Zander, Thomas, ...
Anirban Maitra, Ignacio I. Wistuba, Constance Washington, Arvind K. Virmani, Raheela Ashfaq, Sara Milchgrub, ...
We performed high-resolution allelotyping for loss of heterozygosity (LOH) analysis on microdissected samples from 45 primary breast cancers, 47 mammary preneoplastic epithelial foci, and 18 breast...
Characterizing the cancer genome in lung adenocarcinoma (2007)
Weir, Barbara A., Woo, Michele S., Getz, Gad, Perner, Sven, Ding, Li, Beroukhim, Rameen, ...
Somatic alterations in cellular DNA underlie almost all human cancers(1). The prospect of targeted therapies(2) and the development of high-resolution, genome-wide approaches(3-8) are now spurring...
Polymorphisms, Mutations, and Amplification of the EGFR Gene in Non-Small Cell Lung Cancers (2007)
Masaharu Nomura, Hisayuki Shigematsu, Lin Li, Makoto Suzuki, Takao Takahashi, Pila Estess, ...
BackgroundThe epidermal growth factor receptor (EGFR) gene is the prototype member of the type I receptor tyrosine kinase (TK) family and plays a pivotal role in cell proliferation and...
Characterizing the cancer genome in lung adenocarcinoma (2007)
Weir, Barbara A., Woo, Michele S., Getz, Gad, Perner, Sven, Ding, Li, Beroukhim, Rameen, ...
Toyooka, Shinichi, Matsuo, Keitaro, Shigematsu, Hisayuki, Kosaka, Takayuki, Tokumo, Masaki, Yatabe, Yasushi, ...
Purpose: Mutation of epidermal growth factor receptor (EGFR) gene has been reported to be present in non–small cell lung cancer (NSCLC) and significantly associated with female sex and...
David S. Shames, Luc Girard, Boning Gao, Mitsuo Sato, Cheryl M. Lewis, Narayan Shivapurkar, ...
BackgroundPromoter hypermethylation coupled with loss of heterozygosity at the same locus results in loss of gene function in many tumor cells. The “rules” governing which genes are methylated...
Shames, David S., Girard, Luc, Gao, Boning, Sato, Mitsuo, Lewis, Cheryl M., Shivapurkar, Narayan, ...
Promoter hypermethylation coupled with loss of heterozygosity at the same locus results in loss of gene function in many tumor cells. The “rules” governing which genes are methylated during the...
Inhibition of EGFR Signaling: All Mutations Are Not Created Equal (2005)
Gazdar and Minna discuss the context and implications of a research article that examines the transformation potential and response to inhibitors of specific EGFR mutations found in lung cancer.
Zöchbauer-Müller, Sabine, Fong, Kwun M., Geradts, Joseph, Xu, Xie, Seidl, Sonja, End-Pfützenreuter, Adelheid, ...
Recently, the human SRBC (hSRBC) gene, a candidate tumor suppressor gene (TSG), has been mapped to the chromosomal region 11p15.5–p15.4 where frequent allele loss has been described in lung cancer....
Shigematsu, Hisayuki, Lin, Li, Takahashi, Takao, Nomura, Masaharu, Suzuki, Makoto, Wistuba, Ignacio I., ...
Background: Mutations in the tyrosine kinase (TK) domain of the epidermal growth factor receptor (EGFR) gene in lung cancers are associated with increased sensitivity of these cancers to drugs that...
Toyooka, Shinichi, Maruyama, Riichiroh, Toyooka, Kiyomi O., McLerran, Dale, Feng, Ziding, Fukuyama, Yasuro, ...
Aberrant methylation of several known or putative tumor suppressor genes occurs frequently during the pathogenesis of lung cancers. There are major smoke exposure, histology, geography and...
Corn, Paul G., Summers, Matthew K., Fogt, Franz, Virmani, Arvind K., Gazdar, Adi F., Halazonetis, Thanos D., ...
Chfr, a mitotic stress checkpoint gene, regulates a prophase delay in cells exposed to agents that disrupt microtubules, such as nocodazole and taxol. In the present study, we report that Chfr is...
A Randomized Phase IIb Trial of Anethole Dithiolethione in Smokers with Bronchial Dysplasia (2002)
Lam, Stephen, MacAulay, Calum, Le Riche, Jean C., Dyachkova, Yulia, Coldman, Andy, Guillaud, Martial, ...
Background: Results from preclinical studies have suggested that the organosulfur compound anethole dithiolethione (ADT) may be an effective chemopreventive agent for lung cancer. We conducted a...
Loss of Heterozygosity in Benign Breast Epithelium in Relation to Breast Cancer Risk (2002)
Euhus, David M., Cler, Leslie, Shivapurkar, Narayan, Milchgrub, Sara, Peters, George N., Leitch, A. Marilyn, ...
The multistage model of breast carcinogenesis suggests that errors in DNA replication and repair generate diversity in the breast epithelium (the mutator phenotype), resulting in selection and...
Gazdar, Adi F., Zöchbauer-Möller, Sabine, Virmani, Arvind, Kurie, Jonathan, Minna, John D., Lam, Stephen
Burbee, David G., Forgacs, Eva, Zöchbauer-Müller, Sabine, Shivakumar, Latha, Fong, Kwun, Gao, Boning, ...
Background: The recently identified RASSF1 locus is located within a 120-kilobase region of chromosome 3p21.3 that frequently undergoes allele loss in lung and breast cancers. We explored the...
Promoter Methylation and Silencing of the Retinoic Acid Receptor-ß Gene in Lung Carcinomas (2000)
Virmani, Arvind K., Rathi, Asha, Zöchbauer-Müller, Sabine, Sacchi, Nicoletta, Fukuyama, Yasuro, Bryant, David, ...
Background: Retinoic acid plays an important role in lung development and differentiation, acting primarily via nuclear receptors encoded by the retinoic acid receptor-&bgr; (RAR&bgr;) gene. Because...
Chromosome 19 Translocation, Overexpression of Notch3, and Human Lung Cancer (2000)
Dang, Thao P., Gazdar, Adi F., Virmani, Arvind K., Sepetavec, Tanya, Hande, Kenneth R., Minna, John D., ...
Sex-Related Differences in Bronchial Epithelial Changes Associated With Tobacco Smoking (1999)
Lam, Stephen, LeRiche, Jean C., Zheng, Yvonne, Coldman, Andrew, MacAulay, Calum, Hawk, Ernest, ...
BACKGROUND: Lung cancer is the most common cause of cancer death in North American women. Because smoking-related changes in the bronchial epithelium and in lung function have not been studied in...
Multiple Clonal Abnormalities in the Bronchial Epithelium of Patients With Lung Cancer (1999)
Park, In-Won, Wistuba, Ignacio I., Maitra, Anirban, Milchgrub, Sara, Virmani, Arvind K., Minna, John D., ...
BACKGROUND: Several molecular changes, including loss of heterozygosity (i.e., deletion of one copy of allelic DNA sequences) and alterations in microsatellite DNA, have been detected early in the...
Chen, Junjie, Silver, Daniel P., Walpita, Deepika, Cantor, Sharon B., Gazdar, Adi F., Tomlinson, Gail, ...
BRCA1 and BRCA2 account for most cases of familial, early onset breast and/or ovarian cancer and encode products that each interact with hRAD51. Results presented here show that BRCA1 and BRCA2...
Sugio, Kenji, Gazdar, Adi F., Albores-Saavedra, Jorge, Kokkinakis, Demetrius M.
Ductal adenocarcinoma, the most common form of pancreatic cancer in humans, is associated with activation of the K-ras oncogene in ∼90% of cases. In contrast, K-ras mutations are found in
Telomerase Activity in Human Breast Tumors (1996)
Hiyama, Eiso, Gollahon, Lauren, Kataoka, Tsuyoshi, Kuroi, Katsumasa, Yokoyama, Takashi, Gazdar, Adi F., ...
Background: The activity of the ribonucleoprotein enzyme telomerase is not detected in normal somatic cells; thus, with each cell division, the ends of chromosomes consisting of the telomeric repeats...
Telomerase Activity in Small-Cell and NonSmall-Cell Lung Cancers (1995)
Hiyama, Keiko, Hiyama, Eiso, Ishioka, Shinichi, Yamakido, Michio, Inai, Kouki, Gazdar, Adi F., ...
Background:Telomerase is an enzyme that adds hexameric TTAGGG nucleotide repeats onto the ends of vertebrate chromosomal DNAs (i.e., telomeres) to compensate for losses that occur with each round of...
Tsai, Chun-Ming, Chang, Kuo-Ting, Perng, Reury-Perng, Mitsudomi, Tetsuya, Chen, Mei-Hui, Kadoyama, Chikabumi, ...
Background: At diagnosis, most small-cell lung cancers (SCLCs) are chemosensitive, whereas non-small-cell lung cancers (NSCLCs) are usually chemoresistant. Activation of ras genes and HER-2/neu...
Winter, Stefan F., Sekido, Yoshitaka, Minna, John D., McIntire, Donald, Johnson, Bruce E., Gazdar, Adi F., ...
Background: The frequency and clinical relevance of human anti-tumor immune responses is not well known, and few target antigens have been identified. Purpose: This study was designed to determine...
Karyotypic Derivation of H9 Cell Line (1993)
Bunn, Paul A., WHANG-PENG, JACQUELINE, GAZDAR, ADI F., MINNA, JOHN D., CARNEY, DESMOND
Binaschi, Monica, Giaccone, Giuseppe, Gazdar, Adi F., De Isabella, Paola, Capranico, Giovanni, ...
Background: Small-cell lung cancer (SCLC) is a highly chemosensitive tumor, but the recurrent disease that is common after initial response is often unresponsive to further chemotherapy. Although the...
Occurrence of p53 Gene Abnormalities in Gastric Carcinoma Tumors and Cells Lines (1991)
Kim, Joo-Hang, Takahashi, Takashi, Chiba, Itsuo, Park, Jae-Gahb, Birrer, Michael J., Roh, Jae Kyung, ...
We explored the state of the p53 gene in gastric cancer. Using one or more methods, we examined 15 specimens from primary carcinomas (14 tumors, one cell line), five cell lines derived from...
Gazdar, Adi F., Steinberg, Seth M., Russell, Edward K., Linnoila, R. Ilona, Oie, Herbert K., Ghosh, Bimal C., ...
We devised a novel clinical protocol for extensive-stage small cell lung cancer (SCLC), selecting chemotherapy whenever possible on the basis of in vitro drug-sensitivity testing (DST) of individual...
Park, Jae-Gahb, Kramer, Barnett S., Lai, Shinn-Liang, Goldstein, Lori J., Gazdar, Adi F.
We compared the in vitro sensitivity patterns to cytotoxic drugs and expression of the multidrug resistance-associated MDR1 gene (also known as PGY1 gene) in four gastric carcinoma cell lines with...
Bliss, David P., Battey, James F., Linnoila, R. Ilona, Birrer, Michael J., Gazdar, Adi F., Johnson, Bruce E.
Hyponatremia in patients with small cell lung cencer can be caused by tumor production of arginine vasopression (AVP) and result in the sundrome of inappropriate antidiuretic hormone. In evaluating...
MDR1 Gene Expression in Lung Cancer (1989)
Lai, Shinn-Liang, Goldstein, Lori J., Gottesman, Michael M., Pastan, Ira, Tsai, Chun-Ming, Johnson, Bruce E., ...
The MDR1 gene (also known as PGY1) is frequently overexpressed in multidrug-resistant cell lines. We investigated the role of MDR1 gene expression in lung cancer by performing RNA slot blot analysis...
Johnson, Bruce E., Whang-Peng, Jacqueline, Naylor, Susan L., Zbar, Berton, Brauch, Hiltrud, Lee, Elaine, ...
In small cell lung carcinoma, one of the short arms of chromosome 3 is typically lost. To investigate chromosome 3 in extrapulmonary small cell carcinoma, we used DNA probes that detect...
Park, Jae-Gahb, Collins, Jerry M., Gazdar, Adi F., Allegra, Carmen J., Steinberg, Seth M., Greene, Raymond F., ...
Reduced folates have been shown to increase the cytotoxicity of 5-fluorouracil (5-FU) by stabilizing the 5-fluoro-2′-deoxyuridine-5′-monophosphate-thymidylate synthase complex, thus increasing...
Tomizawa, Yoshio, Sekido, Yoshitaka, Kondo, Masashi, Gao, Boning, Yokota, Jun, Roche, Joëlle, ...
Semaphorins SEMA3B and its homologue SEMA3F are 3p21.3 candidate tumor suppressor genes (TSGs), the expression of which is frequently lost in lung cancers. To test the TSG candidacy of SEMA3B and...
Poiesz, Bernard J., Ruscetti, Francis W., Gazdar, Adi F., Bunn, Paul A., Minna, John D., Gallo, Robert C.
Retrovirus particles with type C morphology were found in two T-cell lymphoblastoid cell lines, HUT 102 and CTCL-3, and in fresh peripheral blood lymphocytes obtained from a patient with a cutaneous...
Bunn, Paul A., Whang-Peng, Jacqueline, Carney, Desmond N., Schlam, Mark L., Knutsen, Turid, Gazdar, Adi F.
Flow cytometric (FCM) analysis of DNA content was performed on 82 lymph node and peripheral blood specimens from 46 patients with mycosis fungoides and the Sézary syndrome. Overall, 32 of the 46...
Oncornavirus Expression in Human × Mouse Hybrid Cells Segregating Mouse Chromosomes
Minna, John D., Gazdar, Adi F., Iverson, G. Michael, Marshall, Thomas H., Stromberg, Kurt, Wilson, Samuel H.
Human × mouse hybrid clones obtained by fusing transformed human (VA2) cells with embryonic mouse brain cells were tested for their ability to spontaneously express type C virus particles. It had...
Gazdar, Adi F., Russell, Edward K., Minna, John D.
The replication of mouse-tropic and xenotropic strains of murine leukemia virus in human × mouse hybrid cells was investigated. NB-tropic strains of the leukemia virus replicated efficiently in...
Inhibition of EGFR Signaling: All Mutations Are Not Created Equal
Gazdar and Minna discuss the context and implications of a research article that examines the transformation potential and response to inhibitors of specific EGFR mutations found in lung cancer.
Tomizawa, Yoshio, Sekido, Yoshitaka, Kondo, Masashi, Gao, Boning, Yokota, Jun, Roche, Joëlle, ...
Semaphorins SEMA3B and its homologue SEMA3F are 3p21.3 candidate tumor suppressor genes (TSGs), the expression of which is frequently lost in lung cancers. To test the TSG candidacy of SEMA3B and...
Poiesz, Bernard J., Ruscetti, Francis W., Gazdar, Adi F., Bunn, Paul A., Minna, John D., Gallo, Robert C.
Retrovirus particles with type C morphology were found in two T-cell lymphoblastoid cell lines, HUT 102 and CTCL-3, and in fresh peripheral blood lymphocytes obtained from a patient with a cutaneous...
Bunn, Paul A., Whang-Peng, Jacqueline, Carney, Desmond N., Schlam, Mark L., Knutsen, Turid, Gazdar, Adi F.
Flow cytometric (FCM) analysis of DNA content was performed on 82 lymph node and peripheral blood specimens from 46 patients with mycosis fungoides and the Sézary syndrome. Overall, 32 of the 46...
Oncornavirus Expression in Human × Mouse Hybrid Cells Segregating Mouse Chromosomes
Minna, John D., Gazdar, Adi F., Iverson, G. Michael, Marshall, Thomas H., Stromberg, Kurt, Wilson, Samuel H.
Human × mouse hybrid clones obtained by fusing transformed human (VA2) cells with embryonic mouse brain cells were tested for their ability to spontaneously express type C virus particles. It had...
Gazdar, Adi F., Russell, Edward K., Minna, John D.
The replication of mouse-tropic and xenotropic strains of murine leukemia virus in human × mouse hybrid cells was investigated. NB-tropic strains of the leukemia virus replicated efficiently in...
Inhibition of EGFR Signaling: All Mutations Are Not Created Equal
Gazdar and Minna discuss the context and implications of a research article that examines the transformation potential and response to inhibitors of specific EGFR mutations found in lung cancer.
Shames, David S, Girard, Luc, Gao, Boning, Sato, Mitsuo, Lewis, Cheryl M, Shivapurkar, Narayan, ...
John Minna and colleagues report that a group of genes are commonly methylated in primary lung, breast, colon, and prostate cancer.
Polymorphisms, Mutations, and Amplification of the EGFR Gene in Non-Small Cell Lung Cancers
Nomura, Masaharu, Shigematsu, Hisayuki, Li, Lin, Suzuki, Makoto, Takahashi, Takao, Estess, Pila, ...
Masaharu Nomura and colleagues examine the distribution ofEGFR polymorphisms in different populations and find differences that might explain different responses to tyrosine kinase inhibitors in lung...
Maitra, Anirban, Wistuba, Ignacio I., Washington, Constance, Virmani, Arvind K., Ashfaq, Raheela, Milchgrub, Sara, ...
We performed high-resolution allelotyping for loss of heterozygosity (LOH) analysis on microdissected samples from 45 primary breast cancers, 47 mammary preneoplastic epithelial foci, and 18 breast...
Toyooka, Kiyomi O., Toyooka, Shinichi, Maitra, Anirban, Feng, Qinghua, Kiviat, Nancy C., Smith, Alice, ...
Aberrant methylation of the promoter region has emerged as the major mechanism for silencing tumor suppressor genes. However, for some genes, such as E-cadherin (CDH1), methylation and protein...
Fragile Histidine Triad Gene Abnormalities in the Pathogenesis of Gallbladder Carcinoma
Wistuba, Ignacio I., Ashfaq, Raheela, Maitra, Anirban, Alvarez, Hector, Riquelme, Erick, Gazdar, Adi F.
There is limited information about the molecular changes involved in the pathogenesis of gallbladder carcinoma (GBC). Our recent allelotyping analyses have indicated that chromosome 3p loss of...
IMMUNE CELL COOPERATION, VIRUSES, AND ANTIBODIES TO NUCLEIC ACIDS IN NEW ZEALAND MICE
Talal, Norman, Steinberg, Alfred D., Jacobs, Michael E., Chused, Thomas M., Gazdar, Adi F.
The development of autoimmunity in New Zealand mice is related to genetic, immunologic, and viral factors. Evidence is presented to suggest that thymus-dependent immune functions may be depressed and...
In Vitro Study of the Biology of Small Cell Carcinoma of the Lung
Gazdar, Adi F., Carney, Desmond N., Minna, John D.
We have developed three types of experimental systems for the study of SCCL: (1) serially heterotransplanted tumors in athymic nude mice; (2) continuous, clonable cell cultures; and (3) direct...
A noncoding RNA gene on chromosome 10p15.3 may function upstream of hTERT
Miura, Norimasa, Sato, Reina, Tsukamoto, Tomoe, Shimizu, Mika, Kabashima, Hiroko, Takeda, Miho, ...
Predicting drug susceptibility of non–small cell lung cancers based on genetic lesions
Sos, Martin L., Michel, Kathrin, Zander, Thomas, Weiss, Jonathan, Frommolt, Peter, Peifer, Martin, ...
Somatic genetic alterations in cancers have been linked with response to targeted therapeutics by creation of specific dependency on activated oncogenic signaling pathways. However, no tools...