Akihiko Kikuchi

PCNA Mono-Ubiquitination and Activation of Translesion DNA Polymerases by DNA Polymerase {alpha} (2009)

Suzuki, Motoshi, Niimi, Atsuko, Limsirichaikul, Siripan, Tomida, Shuta, Miao Huang, Qin, Izuta, Shunji, ...

Translesion DNA synthesis (TLS) involves PCNA mono-ubiquitination and TLS DNA polymerases (pols). Recent evidence has shown that the mono-ubiquitination is induced not only by DNA damage but also by...

Nanostructured designs of biomedical materials: applications of cell sheet engineering to functional regenerative tissues and organs (2005)

Kikuchi, Akihiko, Okano, Teruo

Biomaterials surface design is critical for control of cell–materials interactions. Materials surface characteristics important to cell–materials interactions are the following: (a) nonfouling...

The {alpha}4 residues of human DNA topoisomerase II{alpha} function in enzymatic activity and anticancer drug sensitivity (2004)

Suda, Namiko, Ito, Yasutomo, Imai, Tsuneo, Kikumori, Toyone, Kikuchi, Akihiko, Nishiyama, Yukihiro, ...

We introduced a series of Pro substitutions within and near the α4 helix, a part of the breakage/rejoining region, in human DNA topoisomerase IIα, and analyzed if this region is involved in...

Epitope Distribution of Randomly Established Monoclonal Antibodies against Human Type II DNA Topoisomerases (2002)

Sakaguchi, Ayako, Miyaike, Mitsuko, Kuroda, Kazufumi, Nozaki, Naohito, Tanaka, Masato, Hibino, Makoto, ...

The epitopes of about 100 monoclonal antibodies against human type II DNA topoi-somerase were mapped along the enzyme molecules. Although they were randomly and independently established, epitope...

Characterization of cDNA encoding the mouse DNA topoisomerase II that can complement the budding yeast top2 mutation (1992)

Adachi, Noritaka, Miyaike, Mitsuko, Ikeda, Hideo, Kikuchi, Akihiko

Several cDNA clones encoding mouse DNA topoisomerase II were obtained from a mouse spermatocyte cDNA library and the entire coding sequence of the gene was determined. The mouse DNA topoisomerase II...

In Vitro Synthesis of Ornithine Transcarbamylase (1978)

DOHI, Michio, INOUYE, Hiroshi, KIKUCHI, Akihiko, GORINI, Luigi

An in vitro system for the synthesis of ornithine transcarbamylase (OTCase) was established using iS-30 extract from E. coli MDS6−2(“) and DNA of a “ transducing phage carrying argI and argF...

Some Regulation Profiles of Ornithine Transcarbamylase Synthesis In Vitro (1978)

DOHI, Michio, KIKUCHI, Akihiko, GORINI, Luigi

The regulation profiles of OTCase (argF, argI) synthesis in vitro were investigated by using the in vitro system described in the accompanying paper. Addition of 2.6 mM arginine, crude repressor and...

Sperm Chromatin Decondensation by Template Activating Factor I through Direct Interaction with Basic Proteins

Matsumoto, Ken, Nagata, Kyosuke, Miyaji-Yamaguchi, Mary, Kikuchi, Akihiko, Tsujimoto, Masafumi

Template activating factor I (TAF-I) was originally identified as a host factor required for DNA replication and transcription of adenovirus genome complexed with viral basic proteins. Purified TAF-I...

Involvement of Nucleocytoplasmic Shuttling of Yeast Nap1 in Mitotic Progression

Miyaji-Yamaguchi, Mary, Kato, Kohsuke, Nakano, Ryosuke, Akashi, Tomohiro, Kikuchi, Akihiko, Nagata, Kyosuke

Nucleosome assembly protein 1 (Nap1) is widely conserved from yeasts to humans and facilitates nucleosome formation in vitro as a histone chaperone. Nap1 is generally localized in the cytoplasm,...

The α4 residues of human DNA topoisomerase IIα function in enzymatic activity and anticancer drug sensitivity

Suda, Namiko, Ito, Yasutomo, Imai, Tsuneo, Kikumori, Toyone, Kikuchi, Akihiko, Nishiyama, Yukihiro, ...

We introduced a series of Pro substitutions within and near the α4 helix, a part of the breakage/rejoining region, in human DNA topoisomerase IIα, and analyzed if this region is involved in...

Reverse gyrase; ATP-dependent type I topoisomerase from Sulfolobus

Nakasu, Syo, Kikuchi, Akihiko

Reverse gyrase, a topoisomerase which introduces positive superhelical turns into DNA, has been purified from Sulfolobus to near homogeneity. It is a single polypeptide with a mol. wt. of 120 000 as...

Sequence Variation in the T-Cell Epitopes of the Plasmodium falciparum Circumsporozoite Protein among Field Isolates Is Temporally Stable: a 5-Year Longitudinal Study in Southern Vietnam

Jalloh, Amadu, Van Thien, Huynh, Ferreira, Marcelo U., Ohashi, Jun, Matsuoka, Hiroyuki, Kanbe, Toshio, ...

In an effort to decipher the nature and extent of antigen polymorphisms of malaria parasites in a setting where malaria is hypomesoendemic, we conducted a 5-year longitudinal study (1998 to 2003) by...

Yeast Nap1-binding protein Nbp2p is required for mitotic growth at high temperatures and for cell wall integrity.

Ohkuni, Kentaro, Okuda, Asuko, Kikuchi, Akihiko

Nbp2p is a Nap1-binding protein in Saccharomyces cerevisiae identified by its interaction with Nap1 by a two-hybrid system. NBP2 encodes a novel protein consisting of 236 amino acids with a Src...

Sperm Chromatin Decondensation by Template Activating Factor I through Direct Interaction with Basic Proteins

Matsumoto, Ken, Nagata, Kyosuke, Miyaji-Yamaguchi, Mary, Kikuchi, Akihiko, Tsujimoto, Masafumi

Template activating factor I (TAF-I) was originally identified as a host factor required for DNA replication and transcription of adenovirus genome complexed with viral basic proteins. Purified TAF-I...

Involvement of Nucleocytoplasmic Shuttling of Yeast Nap1 in Mitotic Progression

Miyaji-Yamaguchi, Mary, Kato, Kohsuke, Nakano, Ryosuke, Akashi, Tomohiro, Kikuchi, Akihiko, Nagata, Kyosuke

Nucleosome assembly protein 1 (Nap1) is widely conserved from yeasts to humans and facilitates nucleosome formation in vitro as a histone chaperone. Nap1 is generally localized in the cytoplasm,...

The α4 residues of human DNA topoisomerase IIα function in enzymatic activity and anticancer drug sensitivity

Suda, Namiko, Ito, Yasutomo, Imai, Tsuneo, Kikumori, Toyone, Kikuchi, Akihiko, Nishiyama, Yukihiro, ...

We introduced a series of Pro substitutions within and near the α4 helix, a part of the breakage/rejoining region, in human DNA topoisomerase IIα, and analyzed if this region is involved in...

Reverse gyrase; ATP-dependent type I topoisomerase from Sulfolobus

Nakasu, Syo, Kikuchi, Akihiko

Reverse gyrase, a topoisomerase which introduces positive superhelical turns into DNA, has been purified from Sulfolobus to near homogeneity. It is a single polypeptide with a mol. wt. of 120 000 as...

Sequence Variation in the T-Cell Epitopes of the Plasmodium falciparum Circumsporozoite Protein among Field Isolates Is Temporally Stable: a 5-Year Longitudinal Study in Southern Vietnam

Jalloh, Amadu, Van Thien, Huynh, Ferreira, Marcelo U., Ohashi, Jun, Matsuoka, Hiroyuki, Kanbe, Toshio, ...

In an effort to decipher the nature and extent of antigen polymorphisms of malaria parasites in a setting where malaria is hypomesoendemic, we conducted a 5-year longitudinal study (1998 to 2003) by...

Yeast Nap1-binding protein Nbp2p is required for mitotic growth at high temperatures and for cell wall integrity.

Ohkuni, Kentaro, Okuda, Asuko, Kikuchi, Akihiko

Nbp2p is a Nap1-binding protein in Saccharomyces cerevisiae identified by its interaction with Nap1 by a two-hybrid system. NBP2 encodes a novel protein consisting of 236 amino acids with a Src...

Cell Cycle–coupled Relocation of Types I and II Topoisomerases and Modulation of Catalytic Enzyme Activities

Meyer, Kay N., Kjeldsen, Eigil, Straub, Tobias, Knudsen, Birgitta R., Hickson, Ian D., Kikuchi, Akihiko, ...

We visualized DNA topoisomerases in A431 cells and isolated chromosomes by isoenzyme-selective immunofluorescence microscopy. In interphase, topoisomerase I mainly had a homogeneous nuclear...

Conditionally immortalized brain capillary endothelial cell lines established from a transgenic mouse harboring temperature-sensitive simian virus 40 large T-antigen gene

Hosoya, Ken-ichi, Tetsuka, Kazuhiro, Nagase, Katsuhiko, Tomi, Masatoshi, Saeki, Shigeki, Ohtsuki, Sumio, ...

Five immortalized brain capillary endothelial cell lines (TM-BBB1-5) were established from 3 transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene (Tg mouse). These...