Yamashita, Akio, Izumi, Natsuko, Kashima, Isao, Ohnishi, Tetsuo, Saari, Bonnie, Katsuhata, Yukiko, ...
Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism that detects and degrades mRNAs containing premature translation termination codons (PTCs). SMG-1 and Upf1 transiently form a...
Agawa, Yasuo, Sarhan, Moustafa, Kageyama, Yuji, Akagi, Kazutaka, Takai, Masayoshi, Hashiyama, Kazuya, ...
Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-fl is expressed in a temporally regulated manner following pulses of ecdysteroid...
Tasaki, Takafumi, Mulder, Lubbertus C. F., Iwamatsu, Akihiro, Lee, Min Jae, Davydov, Ilia V., Varshavsky, Alexander, ...
A subset of proteins targeted by the N-end rule pathway bear degradation signals called N-degrons, whose determinants include destabilizing N-terminal residues. Our previous work identified mouse...
Tasaki, Takafumi, Mulder, Lubbertus C. F., Iwamatsu, Akihiro, Lee, Min Jae, Davydov, Ilia V., Varshavsky, Alexander, ...
A subset of proteins targeted by the N-end rule pathway bear degradation signals called N-degrons, whose determinants include destabilizing N-terminal residues. Our previous work identified mouse...
Shimojima, Tsukasa, Okada, Masahiro, Nakayama, Takahiro, Ueda, Hitoshi, Okawa, Katsuya, Iwamatsu, Akihiro, ...
Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates...
Nitric Oxide Inactivates Glyoxalase I in Cooperation with Glutathione (2000)
Mitsumoto, Atsushi, Kim, Kwi-Ryeon, Oshima, Genichiro, Kunimoto, Manabu, Okawa, Katsuya, Iwamatsu, Akihiro, ...
We previously found that glyoxalase I (Glo I) is inactivated upon exposure of human endothelial cells to extracellular nitric oxide (NO), and this event correlates with an increase in its pI on...
Kim, Hee-Jung, Tamanoue, Yoshiaki, Jeohn, Gwang-Ho, Iwamatsu, Akihiro, Yokota, Akira, Kim, Yong-Tae, ...
An extracellular metalloprotease was purified from the culture supernatant of Pseudomonas fluorescens strain KT1 to apparent homogeneity and shown to consist of a single polypeptide chain (Mr...
Ihara, Sayoka, Iwamatsu, Akihiro, Fujiyoshi, Toshihide, Komi, Akiko, Yamori, Takao, Fukui, Yasuhisa
We have screened for a factor that induces neurite outgrowth by PC12 cells only after NGF pretreatment in the supernatants of 38 transformed cell lines, finding three positive clones. The factor...
Iwamatsu, Akihiro, Yoshida-Kubomura, Nobuko
The sequential in situ digestion of proteins immobilized on polyvinylidene difluoride (PVDF) has been systematically designed and optimized. The method consists of immobilization of the proteins on...
Purification and Characterization of Thrombopoietin (1995)
Kato, Takashi, Ogami, Kinya, Shimada, Yoshihiro, Iwamatsu, Akihiro, Sohma, Yoshiaki, Akahori, Hiromichi, ...
A thrombopoietic factor, termed thrombopoietin (TPO), was highly purified directly from the plasma of sublethally irradiated 1, 100 rats by measuring the production of megakaryo-cytes from a highly...
Purification and Characterization of Galactocerebrosidase from Human Lymphocytes (1994)
Sakai, Norio, Inui, Koji, Midorikawa, Mitsuo, Okuno, Yoshinobu, Ueda, Shigeharu, Iwamatsu, Akihiro, ...
Galactocerebrosidase was purified about 22, 600-fold using several hydrophobic column and gel filtration steps with a 4.8% recovery, from human lymphocytes. Its specific activity was 1.54 � 105...
Cloning of the gene for monogalactosyldiacylglycerol synthase and its evolutionary origin
Shimojima, Mie, Ohta, Hiroyuki, Iwamatsu, Akihiro, Masuda, Tatsuru, Shioi, Yuzo, Takamiya, Ken-ichiro
Monogalactosyldiacylglycerol (MGDG) synthase (UDPgalactose:1,2-diacylglycerol 3-β-d-galactosyltransferase; EC 2.4.1.46) catalyzes formation of MGDG, a major structural lipid of chloroplast. We...
The structure and function of a soybean β-glucan-elicitor-binding protein
Umemoto, Naoyuki, Kakitani, Makoto, Iwamatsu, Akihiro, Yoshikawa, Masaaki, Yamaoka, Naoto, Ishida, Isao
β-Glucan elicitor (GE), released from the cell wall of the phytopathogenic fungus Phytophthora megasperma by soybean glucanases, causes defense reactions in soybean. A GE-binding protein (GEBP) was...
Tsuchiya, Tohru, Ohta, Hiroyuki, Okawa, Katsuya, Iwamatsu, Akihiro, Shimada, Hiroshi, Masuda, Tatsuru, ...
Chlorophyllase (Chlase) is the first enzyme involved in chlorophyll (Chl) degradation and catalyzes the hydrolysis of ester bond to yield chlorophyllide and phytol. In the present study, we isolated...
Mazaki, Yuichi, Hashimoto, Shigeru, Okawa, Katsuya, Tsubouchi, Asako, Nakamura, Kuniaki, Yagi, Ryohei, ...
Paxillin acts as an adaptor protein in integrin signaling. We have shown that paxillin exists in a relatively large cytoplasmic pool, including perinuclear areas, in addition to focal complexes...
Momose, Fumitaka, Basler, Christopher F., O'Neill, Robert E., Iwamatsu, Akihiro, Palese, Peter, Nagata, Kyosuke
Previous biochemical data identified a host cell fraction, designated RAF-2, which stimulated influenza virus RNA synthesis. A 48-kDa polypeptide (RAF-2p48), a cellular splicing factor belonging to...
Shimojima, Tsukasa, Okada, Masahiro, Nakayama, Takahiro, Ueda, Hitoshi, Okawa, Katsuya, Iwamatsu, Akihiro, ...
Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates...
Athauda, Senarath B. P., Matsumoto, Koji, Rajapakshe, Sanath, Kuribayashi, Masayuki, Kojima, Masaki, Kubomura-Yoshida, Nobuko, ...
Carnivorous plants are known to secrete acid proteinases to digest prey, mainly insects, for nitrogen uptake. In the present study, we have purified, for the first time, to homogeneity two acid...
A Family of Mammalian E3 Ubiquitin Ligases That Contain the UBR Box Motif and Recognize N-Degrons
Tasaki, Takafumi, Mulder, Lubbertus C. F., Iwamatsu, Akihiro, Lee, Min Jae, Davydov, Ilia V., Varshavsky, Alexander, ...
A subset of proteins targeted by the N-end rule pathway bear degradation signals called N-degrons, whose determinants include destabilizing N-terminal residues. Our previous work identified mouse...
Role of Numb in Dendritic Spine Development with a Cdc42 GEF Intersectin and EphB2D⃞
Nishimura, Takashi, Yamaguchi, Tomoya, Tokunaga, Akinori, Hara, Akitoshi, Hamaguchi, Tomonari, Kato, Katsuhiro, ...
Numb has been implicated in cortical neurogenesis during nervous system development, as a result of its asymmetric partitioning and antagonizing Notch signaling. Recent studies have revealed that...
Cloning of the gene for monogalactosyldiacylglycerol synthase and its evolutionary origin
Shimojima, Mie, Ohta, Hiroyuki, Iwamatsu, Akihiro, Masuda, Tatsuru, Shioi, Yuzo, Takamiya, Ken-ichiro
Monogalactosyldiacylglycerol (MGDG) synthase (UDPgalactose:1,2-diacylglycerol 3-β-d-galactosyltransferase; EC 2.4.1.46) catalyzes formation of MGDG, a major structural lipid of chloroplast. We...
The structure and function of a soybean β-glucan-elicitor-binding protein
Umemoto, Naoyuki, Kakitani, Makoto, Iwamatsu, Akihiro, Yoshikawa, Masaaki, Yamaoka, Naoto, Ishida, Isao
β-Glucan elicitor (GE), released from the cell wall of the phytopathogenic fungus Phytophthora megasperma by soybean glucanases, causes defense reactions in soybean. A GE-binding protein (GEBP) was...
Tsuchiya, Tohru, Ohta, Hiroyuki, Okawa, Katsuya, Iwamatsu, Akihiro, Shimada, Hiroshi, Masuda, Tatsuru, ...
Chlorophyllase (Chlase) is the first enzyme involved in chlorophyll (Chl) degradation and catalyzes the hydrolysis of ester bond to yield chlorophyllide and phytol. In the present study, we isolated...
Mazaki, Yuichi, Hashimoto, Shigeru, Okawa, Katsuya, Tsubouchi, Asako, Nakamura, Kuniaki, Yagi, Ryohei, ...
Paxillin acts as an adaptor protein in integrin signaling. We have shown that paxillin exists in a relatively large cytoplasmic pool, including perinuclear areas, in addition to focal complexes...
Momose, Fumitaka, Basler, Christopher F., O'Neill, Robert E., Iwamatsu, Akihiro, Palese, Peter, Nagata, Kyosuke
Previous biochemical data identified a host cell fraction, designated RAF-2, which stimulated influenza virus RNA synthesis. A 48-kDa polypeptide (RAF-2p48), a cellular splicing factor belonging to...
Shimojima, Tsukasa, Okada, Masahiro, Nakayama, Takahiro, Ueda, Hitoshi, Okawa, Katsuya, Iwamatsu, Akihiro, ...
Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates...
Athauda, Senarath B. P., Matsumoto, Koji, Rajapakshe, Sanath, Kuribayashi, Masayuki, Kojima, Masaki, Kubomura-Yoshida, Nobuko, ...
Carnivorous plants are known to secrete acid proteinases to digest prey, mainly insects, for nitrogen uptake. In the present study, we have purified, for the first time, to homogeneity two acid...
A Family of Mammalian E3 Ubiquitin Ligases That Contain the UBR Box Motif and Recognize N-Degrons
Tasaki, Takafumi, Mulder, Lubbertus C. F., Iwamatsu, Akihiro, Lee, Min Jae, Davydov, Ilia V., Varshavsky, Alexander, ...
A subset of proteins targeted by the N-end rule pathway bear degradation signals called N-degrons, whose determinants include destabilizing N-terminal residues. Our previous work identified mouse...
Role of Numb in Dendritic Spine Development with a Cdc42 GEF Intersectin and EphB2D⃞
Nishimura, Takashi, Yamaguchi, Tomoya, Tokunaga, Akinori, Hara, Akitoshi, Hamaguchi, Tomonari, Kato, Katsuhiro, ...
Numb has been implicated in cortical neurogenesis during nervous system development, as a result of its asymmetric partitioning and antagonizing Notch signaling. Recent studies have revealed that...
Baba, Masaya, Hong, Seung-Beom, Sharma, Nirmala, Warren, Michelle B., Nickerson, Michael L., Iwamatsu, Akihiro, ...
Birt–Hogg–Dubé syndrome, a hamartoma disorder characterized by benign tumors of the hair follicle, lung cysts, and renal neoplasia, is caused by germ-line mutations in the BHD(FLCN) gene, which...
Kamon, Hokuto, Kawabe, Takaya, Kitamura, Hidemitsu, Lee, Jihye, Kamimura, Daisuke, Kaisho, Tsuneyasu, ...
Dendritic cells (DC) play a central role in immune responses by presenting antigenic peptides to CD4+ T cells through MHCII molecules. Here, we demonstrate a TRIF–GEFH1–RhoB pathway is involved...
Nagata-Ohashi, Kyoko, Ohta, Yusaku, Goto, Kazumichi, Chiba, Shuhei, Mori, Reiko, Nishita, Michiru, ...
Cofilin mediates lamellipodium extension and polarized cell migration by stimulating actin filament dynamics at the leading edge of migrating cells. Cofilin is inactivated by phosphorylation at Ser-3...
Agawa, Yasuo, Sarhan, Moustafa, Kageyama, Yuji, Akagi, Kazutaka, Takai, Masayoshi, Hashiyama, Kazuya, ...
Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-f1 is expressed in a temporally regulated manner following pulses of ecdysteroid...
Martin, Ona C., Gunawardane, Ruwanthi N., Iwamatsu, Akihiro, Zheng, Yixian
Previous studies indicate that γ tubulin ring complex (γTuRC) can nucleate microtubule assembly and may be important in centrosome formation. γTuRC contains approximately eight subunits, which we...
The Ras Target AF-6 is a Substrate of the Fam Deubiquitinating Enzyme
Taya, Shinichiro, Yamamoto, Takaharu, Kano, Kyoko, Kawano, Yoji, Iwamatsu, Akihiro, Tsuchiya, Tomoko, ...
The Ras target AF-6 has been shown to serve as one of the peripheral components of cell–cell adhesions, and is thought to participate in cell–cell adhesion regulation downstream of Ras. We here...
Oegema, Karen, Wiese, Christiane, Martin, Ona C., Milligan, Ronald A., Iwamatsu, Akihiro, Mitchison, Timothy J., ...
γ-tubulin exists in two related complexes in Drosophila embryo extracts (Moritz, M., Y. Zheng, B.M. Alberts, and K. Oegema. 1998. J. Cell Biol. 142:1– 12). Here, we report the purification and...
Welch, Matthew D., DePace, Angela H., Verma, Suzie, Iwamatsu, Akihiro, Mitchison, Timothy J.
The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells. The human complex consists of seven subunits which include the actin related proteins Arp2 and Arp3,...
Characterization and Reconstitution of Drosophila γ-Tubulin Ring Complex Subunits
Gunawardane, Ruwanthi N., Martin, Ona C., Cao, Kan, Zhang, Lijun, Dej, Kimberly, Iwamatsu, Akihiro, ...
The γ-tubulin ring complex (γTuRC) is important for microtubule nucleation from the centrosome. In addition to γ-tubulin, the Drosophila γTuRC contains at least six subunits, three of which...
Direct interaction of insulin-like growth factor-1 receptor with leukemia-associated RhoGEF
Taya, Shinichiro, Inagaki, Naoyuki, Sengiku, Hiroaki, Makino, Hiroshi, Iwamatsu, Akihiro, Urakawa, Itaru, ...
Insulin-like growth factor (IGF)-1 plays crucial roles in growth control and rearrangements of the cytoskeleton. IGF-1 binds to the IGF-1 receptor and thereby induces the autophosphorylation of this...
Yamashita, Akio, Izumi, Natsuko, Kashima, Isao, Ohnishi, Tetsuo, Saari, Bonnie, Katsuhata, Yukiko, ...
Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism that detects and degrades mRNAs containing premature translation termination codons (PTCs). SMG-1 and Upf1 transiently form a...