Shikata, Koh, Sasa-Masuda, Taro, Okuno, Yukiko, Waga, Shou, Sugino, Akio
Background: DNA polymerase epsilon (Pol epsilon) is involved in DNA replication, repair, and cell-cycle checkpoint control in eukaryotic cells. Although the roles of replicative Pol alpha and Pol...
Shikata, Koh, Sasa-Masuda, Taro, Okuno, Yukiko, Waga, Shou, Sugino, Akio
Abstract Background DNA polymerase ε (Pol ε) is involved in DNA replication, repair, and cell-cycle checkpoint control in eukaryotic cells. Although the roles of replicative Pol α and Pol δ in...
Furukohri, Asako, Sato, Noriko, Masai, Hisao, Arai, Ken-ichi, Sugino, Akio, Waga, Shou
Dbf4 is a regulatory subunit for the Cdc7 protein kinase that is required for the initiation of eukaryotic DNA replication, but the precise roles of Dbf4-Cdc7 remain to be determined. Here we...
GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast (2003)
Takayama, Yuko, Kamimura, Yoichiro, Okawa, Mariko, Muramatsu, Sachiko, Sugino, Akio, Araki, Hiroyuki
Ohya, Tomoko, Maki, Satoko, Kawasaki, Yasuo, Sugino, Akio
DNA polymerase ϵ (Polϵ) of Saccharomyces cerevisiae is purified as a complex of four polypeptides with molecular masses of >250, 80, 34 (and 31) and 29 kDa as determined by SDS–PAGE....
Kamimura, Yo-Ichiro, Kawasaki, Yasuo, Ohara, Takeshi, Sugino, Akio
Previously, we have purified and characterized DNA helicase III from the yeast Saccharomyces cerevisiae [Shimizu, K. and Sugino, A. (1993) J. BioL Chem. 268,9578–9584]. Here, we have further...
The RFC2 gene encoding a subunit of replication factor C of Saccharomyces cerevisiae (1994)
Noskov, Vladimir, Maki, Satoko, Kawasaki, Yasuo, Leem, Sun-Hee, Ono, Bun-Ichiro, Araki, Hiroyuki, ...
Replication Factor C (RF-C) of Saccharomyces cerevisiae is a complex that consists of several different polypeptides ranging from 120- to 37 kDa (Yoder and Burgers, 1991; Fien and Stillman, 1992),...
Leem, Sun-Hee, Ropp, Philip A., Sugino, Akio
We identified and purified a new DNA polymerase (DNA polymerase IV), which is similar to mammalian DNA polymerase (3, from Saccharomyces cerevisiae and suggested that it is encoded by YCR14C (POLX)...
Araki, Hiroyuki, Hamatake, Robert K., Morrison, Alan, Johnson, Anthony L., Johnston, Leland H., Sugino, Akio
DNA polymerase II purified from Saccharomyces cerevisiae contains polypeptldes with apparent molecular masses of >200, 80, 34, 30 and 29 kDa, the two largest of which (subunlts A and B) are encoded...
Mansson, Per-Erik, Sugino, Akio, Harris, Stephen E.
The abundant class of poly(A$)RNA [poly(A$)RNAllS] from rat seminal vesicle was used to synthesize ds-cDNAllS. The ds-cDNAllS was inserted and cloned into the Pst I site of pBR-322 using E. coil RR1...
Sugino, Akio, Higgins, N.Patrick, Cozzarelli, Nicholas R.
Escherichia coli DNA gyrase contains a 1:1 ratio of protomers coded by the genes gyrA and gyrB. This along with previous results shows that the enzyme has two copies of each protomer and thus a...
Higgins, N. Patrick, Geballe, Adam P., Snopek, Thomas J., Sugino, Akio, Cozzarelli, Nicholas R.
Infection of Escherichia coli by a bacteriophage T4 regA, gene 44 double mutant leads to about a 7-fold increase in the amount of RNA ligase obtained after infection by wild-type phage. Using cells...
Waga, Shou, Masuda, Taro, Takisawa, Haruhiko, Sugino, Akio
DNA polymerase ɛ (Polɛ) is thought to be involved in DNA replication, repair, and cell-cycle checkpoint control in eukaryotic cells. Although the requirement of other replicative DNA polymerases,...
Masumoto, Hiroshi, Sugino, Akio, Araki, Hiroyuki
Dpb11 is required for chromosomal DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae. Here, we report detection of a physical complex containing Dpb11 and DNA polymerase ɛ...
Noskov, Vladimir N., Araki, Hiroyuki, Sugino, Akio
Replication factor C (RF-C), an auxiliary factor for DNA polymerases δ and ɛ, is a multiprotein complex consisting of five different polypeptides. It recognizes a primer on a template DNA, binds to...
Kamimura, Yoichiro, Masumoto, Hiroshi, Sugino, Akio, Araki, Hiroyuki
The DPB11 gene, which genetically interacts with DNA polymerase II (ɛ), one of three replicative DNA polymerases, is required for DNA replication and the S phase checkpoint in Saccharomyces...
Structure and function of the fourth subunit (Dpb4p) of DNA polymerase ɛ in Saccharomyces cerevisiae
Ohya, Tomoko, Maki, Satoko, Kawasaki, Yasuo, Sugino, Akio
DNA polymerase ɛ (Polɛ) of Saccharomyces cerevisiae is purified as a complex of four polypeptides with molecular masses of >250, 80, 34 (and 31) and 29 kDa as determined by SDS–PAGE. The genes...
Shimizu, Kikuo, Kawasaki, Yasuo, Hiraga, Shin-Ichiro, Tawaramoto, Maki, Nakashima, Naomi, Sugino, Akio
We report that POL5 encodes the fifth essential DNA polymerase in Saccharomyces cerevisiae. Pol5p was identified and purified from yeast cell extracts and is an aphidicolin-sensitive DNA polymerase...
Kamimura, Yoichiro, Tak, Yon-Soo, Sugino, Akio, Araki, Hiroyuki
Cdc45, which binds to the minichromosomal maintenance (Mcm) proteins, has a pivotal role in the initiation and elongation steps of chromosomal DNA replication in eukaryotes. Here we show that...
GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast
Takayama, Yuko, Kamimura, Yoichiro, Okawa, Mariko, Muramatsu, Sachiko, Sugino, Akio, Araki, Hiroyuki
Eukaryotic chromosomal DNA replication requires a two-step assembly of replication proteins on origins; formation of the prereplicative complex (pre-RC) in late M and G1 phases of the cell cycle, and...
Mcm2 is a target of regulation by Cdc7–Dbf4 during the initiation of DNA synthesis
Lei, Ming, Kawasaki, Yasuo, Young, Michael R., Kihara, Makoto, Sugino, Akio, Tye, Bik K.
The initiation of DNA synthesis is an important cell cycle event that defines the beginning of S phase. This critical event involves the participation of proteins whose functions are regulated by...
Energy coupling in DNA gyrase and the mechanism of action of novobiocin
Sugino, Akio, Higgins, N. Patrick, Brown, Patrick O., Peebles, Craig L., Cozzarelli, Nicholas R.
Escherichia coli DNA gyrase catalyzes negative supercoiling of closed duplex DNA at the expense of ATP. Two additional activities of the enzyme that have illuminated the energy coupling component of...
Slow Joining of Newly Replicated DNA Chains in DNA Polymerase I-Deficient Escherichia coli Mutants*
Okazaki, Reiji, Arisawa, Mikio, Sugino, Akio
In Escherichia coli mutants deficient in DNA polymerase I, newly replicated short DNA is joined at about 10% of the rate in the wild-type strains. It is postulated that DNA polymerase I normally...
Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic activity
Higgins, N. Patrick, Peebles, Craig L., Sugino, Akio, Cozzarelli, Nicholas R.
Extensively purified DNA gyrase from Escherichia coli is inhibited by nalidixic acid and by novobiocin. The enzyme is composed of two subunits, A and B, which were purified as separate components....
RNA-Linked Nascent DNA Fragments in Escherichia coli*
Sugino, Akio, Hirose, Susumu, Okazaki, Reiji
Nucleic acid that is extracted from E. coli labeled by a brief pulse of [3H]dT and depatured by treatment with heat, formamide, or formaldehyde bands in a region with a density higher than that of...
Sugino, Akio, Peebles, Craig L., Kreuzer, Kenneth N., Cozzarelli, Nicholas R.
A target protein for nalidixic and oxolinic acids in Escherichia coli, the nalA gene product (Pnal), was purified to homogeneity as judged by gel electrophoresis, using an in vitro complementation...
RNA-Linked DNA Fragments In Vitro*
RNA-linked DNA fragments are intermediates in DNA replication in Escherichia coli cells made permeable to nucleoside triphosphates by treatment with toluene. Covalent linkage of a short RNA stretch...
Waga, Shou, Masuda, Taro, Takisawa, Haruhiko, Sugino, Akio
DNA polymerase ɛ (Polɛ) is thought to be involved in DNA replication, repair, and cell-cycle checkpoint control in eukaryotic cells. Although the requirement of other replicative DNA polymerases,...
Masumoto, Hiroshi, Sugino, Akio, Araki, Hiroyuki
Dpb11 is required for chromosomal DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae. Here, we report detection of a physical complex containing Dpb11 and DNA polymerase ɛ...
Noskov, Vladimir N., Araki, Hiroyuki, Sugino, Akio
Replication factor C (RF-C), an auxiliary factor for DNA polymerases δ and ɛ, is a multiprotein complex consisting of five different polypeptides. It recognizes a primer on a template DNA, binds to...
Kamimura, Yoichiro, Masumoto, Hiroshi, Sugino, Akio, Araki, Hiroyuki
The DPB11 gene, which genetically interacts with DNA polymerase II (ɛ), one of three replicative DNA polymerases, is required for DNA replication and the S phase checkpoint in Saccharomyces...
Structure and function of the fourth subunit (Dpb4p) of DNA polymerase ɛ in Saccharomyces cerevisiae
Ohya, Tomoko, Maki, Satoko, Kawasaki, Yasuo, Sugino, Akio
DNA polymerase ɛ (Polɛ) of Saccharomyces cerevisiae is purified as a complex of four polypeptides with molecular masses of >250, 80, 34 (and 31) and 29 kDa as determined by SDS–PAGE. The genes...
Shimizu, Kikuo, Kawasaki, Yasuo, Hiraga, Shin-Ichiro, Tawaramoto, Maki, Nakashima, Naomi, Sugino, Akio
We report that POL5 encodes the fifth essential DNA polymerase in Saccharomyces cerevisiae. Pol5p was identified and purified from yeast cell extracts and is an aphidicolin-sensitive DNA polymerase...
Kamimura, Yoichiro, Tak, Yon-Soo, Sugino, Akio, Araki, Hiroyuki
Cdc45, which binds to the minichromosomal maintenance (Mcm) proteins, has a pivotal role in the initiation and elongation steps of chromosomal DNA replication in eukaryotes. Here we show that...
GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast
Takayama, Yuko, Kamimura, Yoichiro, Okawa, Mariko, Muramatsu, Sachiko, Sugino, Akio, Araki, Hiroyuki
Eukaryotic chromosomal DNA replication requires a two-step assembly of replication proteins on origins; formation of the prereplicative complex (pre-RC) in late M and G1 phases of the cell cycle, and...
Mcm2 is a target of regulation by Cdc7–Dbf4 during the initiation of DNA synthesis
Lei, Ming, Kawasaki, Yasuo, Young, Michael R., Kihara, Makoto, Sugino, Akio, Tye, Bik K.
The initiation of DNA synthesis is an important cell cycle event that defines the beginning of S phase. This critical event involves the participation of proteins whose functions are regulated by...
Energy coupling in DNA gyrase and the mechanism of action of novobiocin
Sugino, Akio, Higgins, N. Patrick, Brown, Patrick O., Peebles, Craig L., Cozzarelli, Nicholas R.
Escherichia coli DNA gyrase catalyzes negative supercoiling of closed duplex DNA at the expense of ATP. Two additional activities of the enzyme that have illuminated the energy coupling component of...
Slow Joining of Newly Replicated DNA Chains in DNA Polymerase I-Deficient Escherichia coli Mutants*
Okazaki, Reiji, Arisawa, Mikio, Sugino, Akio
In Escherichia coli mutants deficient in DNA polymerase I, newly replicated short DNA is joined at about 10% of the rate in the wild-type strains. It is postulated that DNA polymerase I normally...
Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic activity
Higgins, N. Patrick, Peebles, Craig L., Sugino, Akio, Cozzarelli, Nicholas R.
Extensively purified DNA gyrase from Escherichia coli is inhibited by nalidixic acid and by novobiocin. The enzyme is composed of two subunits, A and B, which were purified as separate components....
RNA-Linked Nascent DNA Fragments in Escherichia coli*
Sugino, Akio, Hirose, Susumu, Okazaki, Reiji
Nucleic acid that is extracted from E. coli labeled by a brief pulse of [3H]dT and depatured by treatment with heat, formamide, or formaldehyde bands in a region with a density higher than that of...
Sugino, Akio, Peebles, Craig L., Kreuzer, Kenneth N., Cozzarelli, Nicholas R.
A target protein for nalidixic and oxolinic acids in Escherichia coli, the nalA gene product (Pnal), was purified to homogeneity as judged by gel electrophoresis, using an in vitro complementation...
RNA-Linked DNA Fragments In Vitro*
RNA-linked DNA fragments are intermediates in DNA replication in Escherichia coli cells made permeable to nucleoside triphosphates by treatment with toluene. Covalent linkage of a short RNA stretch...
Fujioka, Yutaka (prod.), Dezaki, Osamu (realización), Yamazaki, Haruya (guión), Ito, Kokyu (guión), Sugie, Keiko (guión), Sugino, Akio (animación), ...
Traducción de: Rittai anime ie naki ko Remi
Fujioka, Yutaka (prod.), Dezaki, Osamu (realización), Yamazaki, Haruya (guión), Ito, Kokyu (guión), Sugie, Keiko (guión), Sugino, Akio (animación), ...
Traducción de: Rittai anime ie naki ko Remi
Fujioka, Yutaka (prod.), Dezaki, Osamu (realización), Yamazaki, Haruya (guión), Ito, Kokyu (guión), Sugie, Keiko (guión), Sugino, Akio (animación), ...
Traducción de: Rittai anime ie naki ko Remi
Fujioka, Yutaka (prod.), Dezaki, Osamu (realización), Yamazaki, Haruya (guión), Ito, Kokyu (guión), Sugie, Keiko (guión), Sugino, Akio (animación), ...
Traducción de: Rittai anime ie naki ko Remi
Fujioka, Yutaka (prod.), Dezaki, Osamu (realización), Yamazaki, Haruya (guión), Ito, Kokyu (guión), Sugie, Keiko (guión), Sugino, Akio (animación), ...
Traducción de: Rittai anime ie naki ko Remi
Fujioka, Yutaka (prod.), Dezaki, Osamu (realización), Yamazaki, Haruya (guión), Ito, Kokyu (guión), Sugie, Keiko (guión), Sugino, Akio (animación), ...
Traducción de: Rittai anime ie naki ko Remi