Holbein, Sandra, Wengi, Agnieszka, Decourty, Laurence, Freimoser, Florian M., Jacquier, Alain, Dichtl, Bernhard
Cordycepin (3′ deoxyadenosine) is a biologically active compound that, when incorporated during RNA synthesis in vitro, provokes chain termination due to the absence of a 3′ hydroxyl moiety. We...
Decourty, Laurence, Saveanu, Cosmin, Rappsilber, Juri, Jacquier, Alain, ...
A genome-wide screen for synthetic lethal (SL) interactions with loss of the nuclear exosome cofactors Rrp47/Lrp1 or Air1 identified 3' --> 35' exonucleases, the THO complex required for mRNP...
Olivier Garcia, Cosmin Saveanu, Melissa Cline, Micheline Fromont-racine, Alain Jacquier, Benno Schwikowski, ...
Summary: We have implemented a graph layout algorithm that exposes Gene Ontology (GO) class structure on the network nodes. It can be used in conjunction with BiNGO plug-in to Cytoscape, which finds...
Lebreton, Alice, Rousselle, Jean-Claude, Lenormand, Pascal, Namane, Abdelkader, Jacquier, Alain, Fromont-Racine, Micheline, ...
During the highly conserved process of eukaryotic ribosome formation, RNA follows a maturation path with well-defined, successive intermediates that dynamically associate with many pre-ribosomal...
Demoinet, Emilie, Jacquier, Alain, Lutfalla, Georges, Fromont-Racine, Micheline
Ribosome biogenesis is a major conserved cellular pathway that requires both ribosomal proteins and many preribosomal factors. Most of the pre-60S factors are recycled into the nucleus; some of them...
Boisset, Sandrine, Geissmann, Thomas, Huntzinger, Eric, Fechter, Pierre, Bendridi, Nadia, Possedko, Maria, ...
RNAIII is the intracellular effector of the quorum-sensing system in Staphylococcus aureus. It is one of the largest regulatory RNAs (514 nucleotides long) that are known to control the expression of...
Cytoplasmic Recycling of 60S Preribosomal Factors Depends on the AAA Protein Drg1 (2007)
Pertschy, Brigitte, Saveanu, Cosmin, Zisser, Gertrude, Lebreton, Alice, Tengg, Martin, Jacquier, Alain, ...
Allelic forms of DRG1/AFG2 confer resistance to the drug diazaborine, an inhibitor of ribosome biogenesis in Saccharomyces cerevisiae. Our results show that the AAA-ATPase Drg1 is essential for 60S...
Garcia, Olivier, Saveanu, Cosmin, Cline, Melissa, Fromont-Racine, Micheline, Jacquier, Alain, Schwikowski, Benno, ...
Summary: We have implemented a graph layout algorithm that exposes Gene Ontology (GO) class structure on the network nodes. It can be used in conjunction with BiNGO plug-in to Cytoscape, which finds...
Gene Ontology-based layout and coloring (2006)
Olivier Garcia, Cosmin Saveanu, Melissa Cline, Micheline Fromont-racine, Alain Jacquier, Benno Schwikowski, ...
doi:10.1093/bioinformatics/btl605 GOlorize: a Cytoscape plug-in for network visualization with
TORCHET, CLAIRE, BADIS, GWENAËL, DEVAUX, FRÉDÉRIC, COSTANZO, GINNY, WERNER, MICHEL, JACQUIER, ALAIN
Conversion of uridines into pseudouridines (Ψs) is the most frequent base modification in ribosomal RNAs (rRNAs). In eukaryotes, the pseudouridylation sites are specified by base-pairing with...
BADIS, GWENAEL, FROMONT-RACINE, MICHELINE, JACQUIER, ALAIN
Ribosomal RNAs contain a number of modified nucleotides. The most abundant nucleotide modifications found within rRNAs fall into two types: 2′-O-ribose methylations and pseudouridylations. In...
Experimental study of the geometrical barrier in type-I superconducting strips (1999)
Castro, Hector, Dutoit, Bertrand, Jacquier, Alain, Baharami, Michel, Rinderer, Leo
We study the energy barrier for flux entry of a geometric origin in type-I superconducting strips using magneto-optical observations of the flux structure. The magnetic field is applied...
Recognition of a conserved class of RNA tetraloops by Saccharomyces cerevisiae RNase III
Chanfreau, Guillaume, Buckle, Malcolm, Jacquier, Alain
Ribonucleases III are double-stranded RNA (dsRNA) endonucleases required for the processing of a large number of prokaryotic and eukaryotic transcripts. Although the specificity of bacterial RNase...
Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps
Saveanu, Cosmin, Bienvenu, David, Namane, Abdelkader, Gleizes, Pierre-Emmanuel, Gas, Nicole, Jacquier, Alain, ...
Eukaryotic ribosome maturation depends on a set of well ordered processing steps. Here we describe the functional characterization of yeast Nog2p (Ynr053cp), a highly conserved nuclear protein. Nog2p...
Human L1 element target-primed reverse transcription in vitro
Cost, Gregory J., Feng, Qinghua, Jacquier, Alain, Boeke, Jef D.
L1 elements are ubiquitous human transposons that replicate via an RNA intermediate. We have reconstituted the initial stages of L1 element transposition in vitro. The reaction requires only the...
Sequential Protein Association with Nascent 60S Ribosomal Particles
Saveanu, Cosmin, Namane, Abdelkader, Gleizes, Pierre-Emmanuel, Lebreton, Alice, Rousselle, Jean-Claude, Noaillac-Depeyre, Jacqueline, ...
Ribosome biogenesis in eukaryotes depends on the coordinated action of ribosomal and nonribosomal proteins that guide the assembly of preribosomal particles. These intermediate particles follow a...
Staphylococcus aureus RNAIII and the endoribonuclease III coordinately regulate spa gene expression
Huntzinger, Eric, Boisset, Sandrine, Saveanu, Cosmin, Benito, Yvonne, Geissmann, Thomas, Namane, Abdelkader, ...
Staphylococcus aureus RNAIII is one of the largest regulatory RNAs, which controls several virulence genes encoding exoproteins and cell-wall-associated proteins. One of the RNAIII effects is the...
BADIS, GWENAEL, FROMONT-RACINE, MICHELINE, JACQUIER, ALAIN
Ribosomal RNAs contain a number of modified nucleotides. The most abundant nucleotide modifications found within rRNAs fall into two types: 2′-O-ribose methylations and pseudouridylations. In...
The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae
TORCHET, CLAIRE, BADIS, GWENAËL, DEVAUX, FRÉDÉRIC, COSTANZO, GINNY, WERNER, MICHEL, JACQUIER, ALAIN
Conversion of uridines into pseudouridines (Ψs) is the most frequent base modification in ribosomal RNAs (rRNAs). In eukaryotes, the pseudouridylation sites are specified by base-pairing with...
Recognition of a conserved class of RNA tetraloops by Saccharomyces cerevisiae RNase III
Chanfreau, Guillaume, Buckle, Malcolm, Jacquier, Alain
Ribonucleases III are double-stranded RNA (dsRNA) endonucleases required for the processing of a large number of prokaryotic and eukaryotic transcripts. Although the specificity of bacterial RNase...
Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps
Saveanu, Cosmin, Bienvenu, David, Namane, Abdelkader, Gleizes, Pierre-Emmanuel, Gas, Nicole, Jacquier, Alain, ...
Eukaryotic ribosome maturation depends on a set of well ordered processing steps. Here we describe the functional characterization of yeast Nog2p (Ynr053cp), a highly conserved nuclear protein. Nog2p...
Human L1 element target-primed reverse transcription in vitro
Cost, Gregory J., Feng, Qinghua, Jacquier, Alain, Boeke, Jef D.
L1 elements are ubiquitous human transposons that replicate via an RNA intermediate. We have reconstituted the initial stages of L1 element transposition in vitro. The reaction requires only the...
Sequential Protein Association with Nascent 60S Ribosomal Particles
Saveanu, Cosmin, Namane, Abdelkader, Gleizes, Pierre-Emmanuel, Lebreton, Alice, Rousselle, Jean-Claude, Noaillac-Depeyre, Jacqueline, ...
Ribosome biogenesis in eukaryotes depends on the coordinated action of ribosomal and nonribosomal proteins that guide the assembly of preribosomal particles. These intermediate particles follow a...
Staphylococcus aureus RNAIII and the endoribonuclease III coordinately regulate spa gene expression
Huntzinger, Eric, Boisset, Sandrine, Saveanu, Cosmin, Benito, Yvonne, Geissmann, Thomas, Namane, Abdelkader, ...
Staphylococcus aureus RNAIII is one of the largest regulatory RNAs, which controls several virulence genes encoding exoproteins and cell-wall-associated proteins. One of the RNAIII effects is the...
BADIS, GWENAEL, FROMONT-RACINE, MICHELINE, JACQUIER, ALAIN
Ribosomal RNAs contain a number of modified nucleotides. The most abundant nucleotide modifications found within rRNAs fall into two types: 2′-O-ribose methylations and pseudouridylations. In...
The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae
TORCHET, CLAIRE, BADIS, GWENAËL, DEVAUX, FRÉDÉRIC, COSTANZO, GINNY, WERNER, MICHEL, JACQUIER, ALAIN
Conversion of uridines into pseudouridines (Ψs) is the most frequent base modification in ribosomal RNAs (rRNAs). In eukaryotes, the pseudouridylation sites are specified by base-pairing with...
Saveanu, Cosmin, Rousselle, Jean-Claude, Lenormand, Pascal, Namane, Abdelkader, Jacquier, Alain, Fromont-Racine, Micheline
Ribosome biogenesis is driven by a large number of preribosomal factors that associate with and dissociate from the preribosomal particles along the maturation pathway. We have previously shown that...
Boisset, Sandrine, Geissmann, Thomas, Huntzinger, Eric, Fechter, Pierre, Bendridi, Nadia, Possedko, Maria, ...
RNAIII is the intracellular effector of the quorum-sensing system in Staphylococcus aureus. It is one of the largest regulatory RNAs (514 nucleotides long) that are known to control the expression of...
A functional network involved in the recycling of nucleocytoplasmic pre-60S factors
Lebreton, Alice, Saveanu, Cosmin, Decourty, Laurence, Rain, Jean-Christophe, Jacquier, Alain, Fromont-Racine, Micheline
Eukaryotic pre-ribosomes go through cytoplasmic maturation steps before entering translation. The nucleocytoplasmic proteins participating in these late stages of maturation are reimported to the...
Cytoplasmic Recycling of 60S Preribosomal Factors Depends on the AAA Protein Drg1▿ †
Pertschy, Brigitte, Saveanu, Cosmin, Zisser, Gertrude, Lebreton, Alice, Tengg, Martin, Jacquier, Alain, ...
Allelic forms of DRG1/AFG2 confer resistance to the drug diazaborine, an inhibitor of ribosome biogenesis in Saccharomyces cerevisiae. Our results show that the AAA-ATPase Drg1 is essential for 60S...
Demoinet, Emilie, Jacquier, Alain, Lutfalla, Georges, Fromont-Racine, Micheline
Ribosome biogenesis is a major conserved cellular pathway that requires both ribosomal proteins and many preribosomal factors. Most of the pre-60S factors are recycled into the nucleus; some of them...
Hmo1 Is Required for TOR-Dependent Regulation of Ribosomal Protein Gene Transcription▿ †
Berger, Axel B., Decourty, Laurence, Badis, Gwenaël, Nehrbass, Ulf, Jacquier, Alain, Gadal, Olivier
Ribosome biogenesis requires equimolar amounts of four rRNAs and all 79 ribosomal proteins (RP). Coordinated regulation of rRNA and RP synthesis by eukaryotic RNA polymerases (Pol) I, III, and II is...
Lebreton, Alice, Rousselle, Jean-Claude, Lenormand, Pascal, Namane, Abdelkader, Jacquier, Alain, Fromont-Racine, Micheline, ...
During the highly conserved process of eukaryotic ribosome formation, RNA follows a maturation path with well-defined, successive intermediates that dynamically associate with many pre-ribosomal...
Decourty, Laurence, Saveanu, Cosmin, Zemam, Kenza, Hantraye, Florence, Frachon, Emmanuel, Rousselle, Jean-Claude, ...
Describing at a genomic scale how mutations in different genes influence one another is essential to the understanding of how genotype correlates with phenotype and remains a major challenge in...
Milligan, Laura, Decourty, Laurence, Saveanu, Cosmin, Rappsilber, Juri, Ceulemans, Hugo, Jacquier, Alain, ...
A genome-wide screen for synthetic lethal (SL) interactions with loss of the nuclear exosome cofactors Rrp47/Lrp1 or Air1 identified 3′→5′ exonucleases, the THO complex required for mRNP...
Holbein, Sandra, Wengi, Agnieszka, Decourty, Laurence, Freimoser, Florian M., Jacquier, Alain, Dichtl, Bernhard
Cordycepin (3′ deoxyadenosine) is a biologically active compound that, when incorporated during RNA synthesis in vitro, provokes chain termination due to the absence of a 3′ hydroxyl moiety. We...