Alain L. Servin

Differential recognition of members of the carcinoembryonic antigen family by Afa/Dr adhesins of diffusely adhering Escherichia coli (Afa/Dr DAEC) (2004)

Berger,Cedric N., Billker,Oliver, Meyer,Thomas F., Servin,Alain L., Kansau,Imad

Little is known about the molecular bases underlying the virulence of diffusely adhering Escherichia coli (DAEC) harbouring the Afa/Dr family of adhesins. These adhesins recognize as receptors the...

Differential recognition of members of the carcinoembryonic antigen family by Afa/Dr adhesins of diffusely adhering Escherichia coli (Afa/Dr DAEC) (2004)

Berger, Cedric N., Billker, Oliver, Meyer, Thomas F., Servin, Alain L., Kansau, Imad

Antagonistic Activity of Lactobacillus acidophilus LB against Intracellular Salmonella enterica Serovar Typhimurium Infecting Human Enterocyte-Like Caco-2/TC-7 Cells

Coconnier, Marie-Hélène, Liévin, Vanessa, Lorrot, Mathie, Servin, Alain L.

To gain further insight into the mechanism by which lactobacilli develop antimicrobial activity, we have examined how Lactobacillus acidophilus LB inhibits the promoted cellular injuries and...

Afa/Dr Diffusely Adhering Escherichia coli C1845 Infection Promotes Selective Injuries in the Junctional Domain of Polarized Human Intestinal Caco-2/TC7 Cells

Peiffer, Isabelle, Blanc-Potard, Anne-Béatrice, Bernet-Camard, Marie-Françoise, Guignot, Julie, Barbat, Alain, Servin, Alain L.

The Afa/Dr diffusely adhering Escherichia coli (DAEC) C1845 strain harboring the F1845 fimbrial adhesin interacts with the brush border-associated CD55 molecule and promotes elongation of brush...

Recruitment of CD55 and CD66e Brush Border-Associated Glycosylphosphatidylinositol-Anchored Proteins by Members of the Afa/Dr Diffusely Adhering Family of Escherichia coli That Infect the Human Polarized Intestinal Caco-2/TC7 Cells

Guignot, Julie, Peiffer, Isabelle, Bernet-Camard, Marie-Françoise, Lublin, Douglas M., Carnoy, Christophe, Moseley, Steve L., ...

The Afa/Dr family of diffusely adhering Escherichia coli (Afa/Dr DAEC) includes bacteria expressing afimbrial adhesins (AFA), Dr hemagglutinin, and fimbrial F1845 adhesin. We show that infection of...

Pyelonephritogenic Diffusely Adhering Escherichia coli EC7372 Harboring Dr-II Adhesin Carries Classical Uropathogenic Virulence Genes and Promotes Cell Lysis and Apoptosis in Polarized Epithelial Caco-2/TC7 Cells

Guignot, Julie, Breard, Jacqueline, Bernet-Camard, Marie-Françoise, Peiffer, Isabelle, Nowicki, Bogdan J., Servin, Alain L., ...

Diffusely adhering Escherichia coli (DAEC) strains expressing adhesins of the Afa/Dr family bind to epithelial cells in a diffuse adherence pattern by recognizing a common receptor, the...

Polarized Entry of Uropathogenic Afa/Dr Diffusely Adhering Escherichia coli Strain IH11128 into Human Epithelial Cells: Evidence for α5β1 Integrin Recognition and Subsequent Internalization through a Pathway Involving Caveolae and Dynamic Unstable Microtubules

Guignot, Julie, Bernet-Camard, Marie-Françoise, Poüs, Christian, Plançon, Laure, Le Bouguenec, Chantal, Servin, Alain L.

Afa/Dr diffusely adhering Escherichia coli strain IH11128 bacteria basolaterally entered polarized epithelial cells by a CD55- and CD66e-independent mechanism through interaction with the α5β1...

Structural and Functional Lesions in Brush Border of Human Polarized Intestinal Caco-2/TC7 Cells Infected by Members of the Afa/Dr Diffusely Adhering Family of Escherichia coli

Peiffer, Isabelle, Guignot, Julie, Barbat, Alain, Carnoy, Christophe, Moseley, Steve L., Nowicki, Bogdan J., ...

Diffusely adhering Escherichia coli (DAEC) strains expressing F1845 fimbrial adhesin or Dr hemagglutinin belonging to the Afa/Dr family of adhesins infect cultured polarized human intestinal cells...

Antagonistic Activity against Helicobacter Infection In Vitro and In Vivo by the Human Lactobacillus acidophilus Strain LB

Coconnier, Marie-Helene, Lievin, Vanessa, Hemery, Elisabeth, Servin, Alain L.

The purpose of the present study was to examine the activity of the human Lactobacillus acidophilus strain LB, which secretes an antibacterial substance(s) against Helicobacter pylori in vitro and in...

Listeria monocytogenes Stimulates Mucus Exocytosis in Cultured Human Polarized Mucosecreting Intestinal Cells through Action of Listeriolysin O

Coconnier, Marie-Hélène, Dlissi, Elyess, Robard, Myriam, Laboisse, Christian L., Gaillard, Jean-Louis, Servin, Alain L.

When the intracellular pathogen Listeria monocytogenes infects cultured human mucosecreting polarized HT29-MTX cells apically, it induces the stimulation of mucus exocytosis without cell entry. Using...

Piracy of Decay-Accelerating Factor (CD55) Signal Transduction by the Diffusely Adhering Strain Escherichia coli C1845 Promotes Cytoskeletal F-Actin Rearrangements in Cultured Human Intestinal INT407 Cells

Peiffer, Isabelle, Servin, Alain L., Bernet-Camard, Marie-Françoise

Diffusely adhering Escherichia coli (DAEC) C1845 (clinical isolate) harboring the fimbrial adhesin F1845 can infect cultured human differentiated intestinal epithelial cells; this process is followed...

Rotavirus Infection Reduces Sucrase-Isomaltase Expression in Human Intestinal Epithelial Cells by Perturbing Protein Targeting and Organization of Microvillar Cytoskeleton

Jourdan, Nathalie, Brunet, Jean Philippe, Sapin, Catherine, Blais, Anne, Cotte-Laffitte, Jacqueline, Forestier, Françoise, ...

Rotavirus infection is the most common cause of severe infantile gastroenteritis worldwide. These viruses infect mature enterocytes of the small intestine and cause structural and functional damage,...

Human Cytomegalovirus Infects Caco-2 Intestinal Epithelial Cells Basolaterally Regardless of the Differentiation State

Esclatine, Audrey, Lemullois, Michel, Servin, Alain L., Quero, Anne-Marie, Geniteau-Legendre, Monique

Human cytomegalovirus (CMV) causes severe disease in immunosuppressed patients and notably infects the gastrointestinal tract. To understand the interaction of CMV with intestinal epithelial cells,...

Rotavirus-Induced Structural and Functional Alterations in Tight Junctions of Polarized Intestinal Caco-2 Cell Monolayers

Obert, Guillaume, Peiffer, Isabelle, Servin, Alain L.

We provide here new insights into rotavirus (RRV) pathogenicity by showing that RRV infection promotes structural and functional injuries localized at the tight junctions (TJ) in the cell-cell...

Representational Difference Analysis between Afa/Dr Diffusely Adhering Escherichia coli and Nonpathogenic E. coli K-12

Blanc-Potard, Anne-Beatrice, Tinsley, Colin, Scaletsky, Isabel, Le Bouguenec, Chantal, Guignot, Julie, Servin, Alain L., ...

Diffusely adhering Escherichia coli strains harboring Afa/Dr adhesins (Afa/Dr DAEC) have been associated with diarrhea and urinary tract infections (UTIs). The present work is the first extensive...

Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells

Minnaard, Jessica, Coconnier, Marie-Helene, Servin, Alain L., Pérez, Pablo F.

In the present study, the role of direct procaryote-eucaryote interactions in the virulence of Bacillus cereus was investigated. As a model of human enterocytes, differentiated Caco-2 cells were...

Zipper-Like Internalization of Dr-Positive Escherichia coli by Epithelial Cells Is Preceded by an Adhesin-Induced Mobilization of Raft-Associated Molecules in the Initial Step of Adhesion

Kansau, Imad, Berger, Cédric, Hospital, Maxime, Amsellem, Raymonde, Nicolas, Valérie, Servin, Alain L., ...

We undertook a study of the mechanism by which Dr-positive bacteria invade epithelial cells. Our findings show that Dr-positive bacteria enter via a zipper-like mechanism that is independent of the...

Human Diffusely Adhering Escherichia coli Expressing Afa/Dr Adhesins That Use Human CD55 (Decay-Accelerating Factor) as a Receptor Does Not Bind the Rodent and Pig Analogues of CD55

Hudault, Sylvie, Spiller, O. Brad, Morgan, B. Paul, Servin, Alain L.

Afa/Dr diffusely adhering Escherichia coli (DAEC) bacteria that are responsible for recurrent urinary tract and gastrointestinal infections recognized as a receptor the glycosylphosphatidylinositol...

Pathogenesis of Afa/Dr Diffusely Adhering Escherichia coli

Servin, Alain L.

Over the last few years, dramatic increases in our knowledge about diffusely adhering Escherichia coli (DAEC) pathogenesis have taken place. The typical class of DAEC includes E. coli strains...

A Lactobacillus acidophilus Strain of Human Gastrointestinal Microbiota Origin Elicits Killing of Enterovirulent Salmonella enterica Serovar Typhimurium by Triggering Lethal Bacterial Membrane Damage

Coconnier-Polter, Marie-Hélène, Servin, Alain L.

The human gastrointestinal microbiota produces antagonistic activities against gastrointestinal bacterial pathogens. We undertook a study to investigate the mechanism(s) by which a Lactobacillus...

pH-, Lactic Acid-, and Non-Lactic Acid-Dependent Activities of Probiotic Lactobacilli against Salmonella enterica Serovar Typhimurium

Fayol-Messaoudi, Domitille, Berger, Cédric N., Coconnier-Polter, Marie-Hélène, Servin, Alain L.

The mechanism(s) underlying the antibacterial activity of probiotic Lactobacillus strains appears to be multifactorial and includes lowering of the pH and the production of lactic acid and of...

Antagonistic Activity of Lactobacillus acidophilus LB against Intracellular Salmonella enterica Serovar Typhimurium Infecting Human Enterocyte-Like Caco-2/TC-7 Cells

Coconnier, Marie-Hélène, Liévin, Vanessa, Lorrot, Mathie, Servin, Alain L.

To gain further insight into the mechanism by which lactobacilli develop antimicrobial activity, we have examined how Lactobacillus acidophilus LB inhibits the promoted cellular injuries and...

Afa/Dr Diffusely Adhering Escherichia coli C1845 Infection Promotes Selective Injuries in the Junctional Domain of Polarized Human Intestinal Caco-2/TC7 Cells

Peiffer, Isabelle, Blanc-Potard, Anne-Béatrice, Bernet-Camard, Marie-Françoise, Guignot, Julie, Barbat, Alain, Servin, Alain L.

The Afa/Dr diffusely adhering Escherichia coli (DAEC) C1845 strain harboring the F1845 fimbrial adhesin interacts with the brush border-associated CD55 molecule and promotes elongation of brush...

Recruitment of CD55 and CD66e Brush Border-Associated Glycosylphosphatidylinositol-Anchored Proteins by Members of the Afa/Dr Diffusely Adhering Family of Escherichia coli That Infect the Human Polarized Intestinal Caco-2/TC7 Cells

Guignot, Julie, Peiffer, Isabelle, Bernet-Camard, Marie-Françoise, Lublin, Douglas M., Carnoy, Christophe, Moseley, Steve L., ...

The Afa/Dr family of diffusely adhering Escherichia coli (Afa/Dr DAEC) includes bacteria expressing afimbrial adhesins (AFA), Dr hemagglutinin, and fimbrial F1845 adhesin. We show that infection of...

Pyelonephritogenic Diffusely Adhering Escherichia coli EC7372 Harboring Dr-II Adhesin Carries Classical Uropathogenic Virulence Genes and Promotes Cell Lysis and Apoptosis in Polarized Epithelial Caco-2/TC7 Cells

Guignot, Julie, Breard, Jacqueline, Bernet-Camard, Marie-Françoise, Peiffer, Isabelle, Nowicki, Bogdan J., Servin, Alain L., ...

Diffusely adhering Escherichia coli (DAEC) strains expressing adhesins of the Afa/Dr family bind to epithelial cells in a diffuse adherence pattern by recognizing a common receptor, the...

Polarized Entry of Uropathogenic Afa/Dr Diffusely Adhering Escherichia coli Strain IH11128 into Human Epithelial Cells: Evidence for α5β1 Integrin Recognition and Subsequent Internalization through a Pathway Involving Caveolae and Dynamic Unstable Microtubules

Guignot, Julie, Bernet-Camard, Marie-Françoise, Poüs, Christian, Plançon, Laure, Le Bouguenec, Chantal, Servin, Alain L.

Afa/Dr diffusely adhering Escherichia coli strain IH11128 bacteria basolaterally entered polarized epithelial cells by a CD55- and CD66e-independent mechanism through interaction with the α5β1...

Structural and Functional Lesions in Brush Border of Human Polarized Intestinal Caco-2/TC7 Cells Infected by Members of the Afa/Dr Diffusely Adhering Family of Escherichia coli

Peiffer, Isabelle, Guignot, Julie, Barbat, Alain, Carnoy, Christophe, Moseley, Steve L., Nowicki, Bogdan J., ...

Diffusely adhering Escherichia coli (DAEC) strains expressing F1845 fimbrial adhesin or Dr hemagglutinin belonging to the Afa/Dr family of adhesins infect cultured polarized human intestinal cells...

Antagonistic Activity against Helicobacter Infection In Vitro and In Vivo by the Human Lactobacillus acidophilus Strain LB

Coconnier, Marie-Helene, Lievin, Vanessa, Hemery, Elisabeth, Servin, Alain L.

The purpose of the present study was to examine the activity of the human Lactobacillus acidophilus strain LB, which secretes an antibacterial substance(s) against Helicobacter pylori in vitro and in...

Listeria monocytogenes Stimulates Mucus Exocytosis in Cultured Human Polarized Mucosecreting Intestinal Cells through Action of Listeriolysin O

Coconnier, Marie-Hélène, Dlissi, Elyess, Robard, Myriam, Laboisse, Christian L., Gaillard, Jean-Louis, Servin, Alain L.

When the intracellular pathogen Listeria monocytogenes infects cultured human mucosecreting polarized HT29-MTX cells apically, it induces the stimulation of mucus exocytosis without cell entry. Using...

Piracy of Decay-Accelerating Factor (CD55) Signal Transduction by the Diffusely Adhering Strain Escherichia coli C1845 Promotes Cytoskeletal F-Actin Rearrangements in Cultured Human Intestinal INT407 Cells

Peiffer, Isabelle, Servin, Alain L., Bernet-Camard, Marie-Françoise

Diffusely adhering Escherichia coli (DAEC) C1845 (clinical isolate) harboring the fimbrial adhesin F1845 can infect cultured human differentiated intestinal epithelial cells; this process is followed...

Rotavirus Infection Reduces Sucrase-Isomaltase Expression in Human Intestinal Epithelial Cells by Perturbing Protein Targeting and Organization of Microvillar Cytoskeleton

Jourdan, Nathalie, Brunet, Jean Philippe, Sapin, Catherine, Blais, Anne, Cotte-Laffitte, Jacqueline, Forestier, Françoise, ...

Rotavirus infection is the most common cause of severe infantile gastroenteritis worldwide. These viruses infect mature enterocytes of the small intestine and cause structural and functional damage,...

Human Cytomegalovirus Infects Caco-2 Intestinal Epithelial Cells Basolaterally Regardless of the Differentiation State

Esclatine, Audrey, Lemullois, Michel, Servin, Alain L., Quero, Anne-Marie, Geniteau-Legendre, Monique

Human cytomegalovirus (CMV) causes severe disease in immunosuppressed patients and notably infects the gastrointestinal tract. To understand the interaction of CMV with intestinal epithelial cells,...

Rotavirus-Induced Structural and Functional Alterations in Tight Junctions of Polarized Intestinal Caco-2 Cell Monolayers

Obert, Guillaume, Peiffer, Isabelle, Servin, Alain L.

We provide here new insights into rotavirus (RRV) pathogenicity by showing that RRV infection promotes structural and functional injuries localized at the tight junctions (TJ) in the cell-cell...

Representational Difference Analysis between Afa/Dr Diffusely Adhering Escherichia coli and Nonpathogenic E. coli K-12

Blanc-Potard, Anne-Beatrice, Tinsley, Colin, Scaletsky, Isabel, Le Bouguenec, Chantal, Guignot, Julie, Servin, Alain L., ...

Diffusely adhering Escherichia coli strains harboring Afa/Dr adhesins (Afa/Dr DAEC) have been associated with diarrhea and urinary tract infections (UTIs). The present work is the first extensive...

Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells

Minnaard, Jessica, Coconnier, Marie-Helene, Servin, Alain L., Pérez, Pablo F.

In the present study, the role of direct procaryote-eucaryote interactions in the virulence of Bacillus cereus was investigated. As a model of human enterocytes, differentiated Caco-2 cells were...

Zipper-Like Internalization of Dr-Positive Escherichia coli by Epithelial Cells Is Preceded by an Adhesin-Induced Mobilization of Raft-Associated Molecules in the Initial Step of Adhesion

Kansau, Imad, Berger, Cédric, Hospital, Maxime, Amsellem, Raymonde, Nicolas, Valérie, Servin, Alain L., ...

We undertook a study of the mechanism by which Dr-positive bacteria invade epithelial cells. Our findings show that Dr-positive bacteria enter via a zipper-like mechanism that is independent of the...

Human Diffusely Adhering Escherichia coli Expressing Afa/Dr Adhesins That Use Human CD55 (Decay-Accelerating Factor) as a Receptor Does Not Bind the Rodent and Pig Analogues of CD55

Hudault, Sylvie, Spiller, O. Brad, Morgan, B. Paul, Servin, Alain L.

Afa/Dr diffusely adhering Escherichia coli (DAEC) bacteria that are responsible for recurrent urinary tract and gastrointestinal infections recognized as a receptor the glycosylphosphatidylinositol...

Pathogenesis of Afa/Dr Diffusely Adhering Escherichia coli

Servin, Alain L.

Over the last few years, dramatic increases in our knowledge about diffusely adhering Escherichia coli (DAEC) pathogenesis have taken place. The typical class of DAEC includes E. coli strains...

A Lactobacillus acidophilus Strain of Human Gastrointestinal Microbiota Origin Elicits Killing of Enterovirulent Salmonella enterica Serovar Typhimurium by Triggering Lethal Bacterial Membrane Damage

Coconnier-Polter, Marie-Hélène, Servin, Alain L.

The human gastrointestinal microbiota produces antagonistic activities against gastrointestinal bacterial pathogens. We undertook a study to investigate the mechanism(s) by which a Lactobacillus...

pH-, Lactic Acid-, and Non-Lactic Acid-Dependent Activities of Probiotic Lactobacilli against Salmonella enterica Serovar Typhimurium

Fayol-Messaoudi, Domitille, Berger, Cédric N., Coconnier-Polter, Marie-Hélène, Servin, Alain L.

The mechanism(s) underlying the antibacterial activity of probiotic Lactobacillus strains appears to be multifactorial and includes lowering of the pH and the production of lactic acid and of...

The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota

Servin, Alain L.

The intestinal tract is a complex ecosystem that combines resident microbiota and the cells of various phenotypes with complex metabolic activities that line the epithelial wall. The intestinal cells...

A Protein Kinase A-Dependent Mechanism by Which Rotavirus Affects the Distribution and mRNA Level of the Functional Tight Junction-Associated Protein, Occludin, in Human Differentiated Intestinal Caco-2 Cells▿

Beau, Isabelle, Cotte-Laffitte, Jacqueline, Amsellem, Raymonde, Servin, Alain L.

We found that at the tight junctions (TJs) of Caco-2 cell monolayers, rhesus monkey rotavirus (RRV) infection induced the disappearance of occludin. Confocal laser scanning microscopy showed the...

Up-Regulation of Intestinal Vascular Endothelial Growth Factor by Afa/Dr Diffusely Adhering Escherichia coli

Cane, Gaëlle, Moal, Vanessa Liévin-Le, Pagès, Gilles, Servin, Alain L., Hofman, Paul, Vouret-Craviari, Valérie

Human Decay-Accelerating Factor and CEACAM Receptor-Mediated Internalization and Intracellular Lifestyle of Afa/Dr Diffusely Adhering Escherichia coli in Epithelial Cells▿

Guignot, Julie, Hudault, Sylvie, Kansau, Imad, Chau, Ingrid, Servin, Alain L.

We used transfected epithelial CHO-B2 cells as a model to identify the mechanism mediating internalization of Afa/Dr diffusely adhering Escherichia coli. We provide evidence that neither the α5 or...