Alan F. Cowman

Sir2 Paralogues Cooperate to Regulate Virulence Genes and Antigenic Variation in Plasmodium falciparum (2009)

Christopher J. Tonkin, Céline K. Carret, Manoj T. Duraisingh, Till S. Voss, Stuart A. Ralph, Mirja Hommel, ...

Cytoadherance of Plasmodium falciparum-infected erythrocytes in the brain, organs and peripheral microvasculature is linked to morbidity and mortality associated with severe malaria. Parasite-derived...

Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites (2009)

Baum, Jake, Papenfuss, Anthony T., Mair, Gunnar R., Janse, Chris J., Vlachou, Dina, Waters, Andrew P., ...

Techniques for targeted genetic disruption in Plasmodium, the causative agent of malaria, are currently intractable for those genes that are essential for blood stage development. The ability to use...

Alveolins, a New Family of Cortical Proteins that Define the Protist Infrakingdom Alveolata (2008)

Gould, Sven B., Tham, Wai-Hong, Cowman, Alan F., McFadden, Geoffrey I., Waller, Ross F.

Alveolates are a recently recognized group of unicellular eukaryotes that unites disparate protists including apicomplexan parasites (which cause malaria and toxoplasmosis), dinoflagellate algae...

Lineage-specific expansion of proteins exported to erythrocytes in malaria parasites (2006)

Sargeant, Tobias J, Marti, Matthias, Caler, Elisabet, Carlton, Jane M, Simpson, Ken, Speed, Terence P, ...

Abstract Background The apicomplexan parasite Plasmodium falciparum causes the most severe form of malaria in humans. After invasion into erythrocytes, asexual parasite stages drastically alter their...

Invasion by P. falciparum Merozoites Suggests a Hierarchy of Molecular Interactions (2005)

Jake Baum, Alexander G. Maier, Robert T. Good, Ken M. Simpson, Alan F. Cowman

Central to the pathology of malaria disease are the repeated cycles of parasite invasion and destruction of human erythrocytes. In Plasmodium falciparum, the most virulent species causing malaria,...

Plasmodium falciparum virulence determinants unveiled (2002)

Crabb, Brendan S, Cowman, Alan F

Abstract The human malaria parasite Plasmodium falciparum, one of the world's most devastating pathogens, has an astonishing array of sequences and genes that play key roles in pathogenesis and...

An alteration in concatameric structure is associated with efficient segregation of plasmids in transfected Plasmodium falciparum parasites (2001)

O’Donnell, Rebecca A., Preiser, Peter R., Williamson, Donald H., Moore, Peter W., Cowman, Alan F., Crabb, Brendan S.

Transfection of the human malaria parasite Plasmodium falciparum is currently performed with circularised plasmids that are maintained episomally in parasites under drug selection but which are...

Targeted disruption of an erythrocyte binding antigen in Plasmodium falciparum is associated with a switch toward a sialic acid-independent pathway of invasion

Reed, Michael B., Caruana, Sonia R., Batchelor, Adrian H., Thompson, Jennifer K., Crabb, Brendan S., Cowman, Alan F.

Erythrocyte invasion by Plasmodium requires molecules present both on the merozoite surface and within the specialized organelles of the apical complex. The Plasmodium erythrocyte binding protein...

clag9: A cytoadherence gene in Plasmodium falciparum essential for binding of parasitized erythrocytes to CD36

Trenholme, Katharine R., Gardiner, Donald L., Holt, Deborah C., Thomas, Elizabeth A., Cowman, Alan F., Kemp, David J.

The propensity of isolates of the malaria parasite Plasmodium falciparum to delete a segment of chromosome 9 has provided positional information that has allowed us to identify a gene necessary for...

The adhesion of Plasmodium falciparum-infected erythrocytes to chondroitin sulfate A is mediated by P. falciparum erythrocyte membrane protein 1

Reeder, John C., Cowman, Alan F., Davern, Kathleen M., Beeson, James G., Thompson, Jennifer K., Rogerson, Stephen J., ...

Chondroitin sulfate A (CSA) is an important receptor for the sequestration of Plasmodium falciparum in the placenta, but the parasite ligand involved in adhesion has not previously been identified....

Nuclear-encoded proteins target to the plastid in Toxoplasma gondii and Plasmodium falciparum

Waller, Ross F., Keeling, Patrick J., Donald, Robert G. K., Striepen, Boris, Handman, Emanuela, Lang-Unnasch, Naomi, ...

A vestigial, nonphotosynthetic plastid has been identified recently in protozoan parasites of the phylum Apicomplexa. The apicomplexan plastid, or “apicoplast,” is indispensable, but the complete...

Mutations in dihydropteroate synthase are responsible for sulfone and sulfonamide resistance in Plasmodium falciparum

Triglia, Tony, Menting, John G. T., Wilson, Craig, Cowman, Alan F.

Plasmodium falciparum causes the most severe form of malaria in humans. An important class of drugs in malaria treatment is the sulfone/sulfonamide group, of which sulfadoxine is the most commonly...

An alteration in concatameric structure is associated with efficient segregation of plasmids in transfected Plasmodium falciparum parasites

O’Donnell, Rebecca A., Preiser, Peter R., Williamson, Donald H., Moore, Peter W., Cowman, Alan F., Crabb, Brendan S.

Transfection of the human malaria parasite Plasmodium falciparum is currently performed with circularised plasmids that are maintained episomally in parasites under drug selection but which are...

Identification of Proteins from Plasmodium falciparum That Are Homologous to Reticulocyte Binding Proteins in Plasmodium vivax

Triglia, Tony, Thompson, Jenny, Caruana, Sonia R., Delorenzi, Mauro, Speed, Terry, Cowman, Alan F.

Plasmodium falciparum infections can be fatal, while P. vivax infections usually are not. A possible factor involved in the greater virulence of P. falciparum is that this parasite grows in red blood...

Plasmodium falciparum Homologue of the Genes for Plasmodium vivax and Plasmodium yoelii Adhesive Proteins, Which Is Transcribed but Not Translated

Taylor, Helen M., Triglia, Tony, Thompson, Jenny, Sajid, Mohammed, Fowler, Ruth, Wickham, Mark E., ...

The 235-kDa family of rhoptry proteins in Plasmodium yoelii and the two reticulocyte binding proteins of P. vivax comprise a family of proteins involved in host cell selection and erythrocyte...

Trafficking and assembly of the cytoadherence complex in Plasmodium falciparum-infected human erythrocytes

Wickham, Mark E., Rug, Melanie, Ralph, Stuart A., Klonis, Nectarios, McFadden, Geoffrey I., Tilley, Leann, ...

After invading human erythrocytes, the malarial parasite Plasmodium falciparum, initiates a remarkable process of secreting proteins into the surrounding erythrocyte cytoplasm and plasma membrane....

A genetic screen for improved plasmid segregation reveals a role for Rep20 in the interaction of Plasmodium falciparum chromosomes

O’Donnell, Rebecca A., Freitas-Junior, Lúcio H., Preiser, Peter R., Williamson, Donald H., Duraisingh, Manoj, McElwain, Terry F., ...

Bacterial plasmids introduced into the human malaria parasite Plasmodium falciparum replicate well but are poorly segregated during mitosis. In this paper, we screened a random P.falciparum genomic...

Identification and Disruption of the Gene Encoding the Third Member of the Low-Molecular-Mass Rhoptry Complex in Plasmodium falciparum

Baldi, Deborah L., Good, Robert, Duraisingh, Manoj T., Crabb, Brendan S., Cowman, Alan F.

The low-molecular-mass rhoptry complex of Plasmodium falciparum consists of three proteins, rhoptry-associated protein 1 (RAP1), RAP2, and RAP3. The genes encoding RAP1 and RAP2 are known; however,...

Independent Translocation of Two Micronemal Proteins in Developing Plasmodium falciparum Merozoites

Healer, Julie, Crawford, Simon, Ralph, Stuart, McFadden, Geoff, Cowman, Alan F.

Apical membrane antigen 1 of Plasmodium falciparum (PfAMA1) contains an N-terminal propeptide that is removed prior to the translocation of the mature protein onto the merozoite surface. We localized...

A Type II Pathway for Fatty Acid Biosynthesis Presents Drug Targets in Plasmodium falciparum

Waller, Ross F., Ralph, Stuart A., Reed, Michael B., Su, Vanessa, Douglas, James D., Minnikin, David E., ...

It has long been held that the malaria parasite, Plasmodium sp., is incapable of de novo fatty acid synthesis. This view has recently been overturned with the emergence of data for the presence of a...

Phenotypic variation of Plasmodium falciparum merozoite proteins directs receptor targeting for invasion of human erythrocytes

Duraisingh, Manoj T., Triglia, Tony, Ralph, Stuart A., Rayner, Julian C., Barnwell, John W., McFadden, Geoffrey I., ...

The members of the phylum Apicomplexa parasitize a wide range of eukaryotic host cells. Plasmodium falciparum, responsible for the most virulent form of malaria, invades human erythrocytes using...

Erythrocyte-binding antigen 175 mediates invasion in Plasmodium falciparum utilizing sialic acid-dependent and -independent pathways

Duraisingh, Manoj T., Maier, Alexander G., Triglia, Tony, Cowman, Alan F.

The Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175) is a ligand for merozoite invasion into human erythrocytes that binds to glycophorin A in a sialic acid-dependent manner. P....

Targeted mutagenesis of Plasmodium falciparum erythrocyte membrane protein 3 (PfEMP3) disrupts cytoadherence of malaria-infected red blood cells

Waterkeyn, Jacqueline G., Wickham, Mark E., Davern, Kathleen M., Cooke, Brian M., Coppel, Ross L., Reeder, John C., ...

Adhesion of parasite-infected red blood cells to the vascular endothelium is a critical event in the pathogenesis of malaria caused by Plasmodium falciparum. Adherence is mediated by the variant...

RAP1 controls rhoptry targeting of RAP2 in the malaria parasite Plasmodium falciparum

Baldi, Deborah L., Andrews, Katherine T., Waller, Ross F., Roos, David S., Howard, Randall F., Crabb, Brendan S., ...

Rhoptry associated protein 1 (RAP1) and 2 (RAP2), together with a poorly described third protein RAP3, form the low molecular weight complex within the rhoptries of Plasmodium falciparum. These...

Plasmodium falciparum virulence determinants unveiled

Crabb, Brendan S, Cowman, Alan F

The human malaria parasite Plasmodium falciparum, has many sequences and genes that play key roles in pathogenesis and immune evasion. We must understand the functions of these elements if the...

Protein trafficking to the plastid of Plasmodium falciparum is via the secretory pathway

Waller, Ross F., Reed, Michael B., Cowman, Alan F., McFadden, Geoffrey I.

The plastid of Plasmodium falciparum (or ‘apicoplast’) is the evolutionary homolog of the plant chloroplast and represents a vestige of a photosynthetic past. Apicoplast indispensability...

Plasmodium falciparum Merozoite Surface Protein 6 Is a Dimorphic Antigen

Pearce, J. Andrew, Triglia, Tony, Hodder, Anthony N., Jackson, David C., Cowman, Alan F., Anders, Robin F.

Merozoite surface protein 1 (MSP1) is a highly polymorphic Plasmodium falciparum merozoite surface protein implicated in the invasion of human erythrocytes during the asexual cycle. It forms a...

Correct Promoter Control Is Needed for Trafficking of the Ring-Infected Erythrocyte Surface Antigen to the Host Cytosol in Transfected Malaria Parasites

Rug, Melanie, Wickham, Mark E., Foley, Michael, Cowman, Alan F., Tilley, Leann

Following invasion of human erythrocytes, the malaria parasite, Plasmodium falciparum, exports proteins beyond the confines of its own plasma membrane to modify the properties of the host red cell...

Functional Analysis of Plasmodium falciparum Apical Membrane Antigen 1 Utilizing Interspecies Domains

Healer, Julie, Triglia, Tony, Hodder, Anthony N., Gemmill, Alan W., Cowman, Alan F.

Plasmodium falciparum apical membrane antigen 1 (AMA1) is a leading malaria vaccine candidate whose function has not been unequivocally defined. Partial complementation of function can be achieved by...

Invasion by P. falciparum Merozoites Suggests a Hierarchy of Molecular Interactions

Baum, Jake, Maier, Alexander G, Good, Robert T, Simpson, Ken M, Cowman, Alan F

Central to the pathology of malaria disease are the repeated cycles of parasite invasion and destruction of human erythrocytes. In Plasmodium falciparum, the most virulent species causing malaria,...

Lineage-specific expansion of proteins exported to erythrocytes in malaria parasites

Sargeant, Tobias J, Marti, Matthias, Caler, Elisabet, Carlton, Jane M, Simpson, Ken, Speed, Terence P, ...

A new software was used to predict exported proteins that are conserved between malaria parasites infecting rodents and those infecting humans, revealing a lineage-specific expansion of exported...

Delivery of the Malaria Virulence Protein PfEMP1 to the Erythrocyte Surface Requires Cholesterol-Rich Domains

Frankland, Sarah, Adisa, Akinola, Horrocks, Paul, Taraschi, Theodore F., Schneider, Timothy, Elliott, Salenna R., ...

The particular virulence of the human malaria parasite Plasmodium falciparum derives from export of parasite-encoded proteins to the surface of the mature erythrocytes in which it resides. The...

Genesis of and Trafficking to the Maurer's Clefts of Plasmodium falciparum-Infected Erythrocytes†

Spycher, Cornelia, Rug, Melanie, Klonis, Nectarios, Ferguson, David J. P., Cowman, Alan F., Beck, Hans-Peter, ...

Malaria parasites export proteins beyond their own plasma membrane to locations in the red blood cells in which they reside. Maurer's clefts are parasite-derived structures within the host cell...

Targeted disruption of an erythrocyte binding antigen in Plasmodium falciparum is associated with a switch toward a sialic acid-independent pathway of invasion

Reed, Michael B., Caruana, Sonia R., Batchelor, Adrian H., Thompson, Jennifer K., Crabb, Brendan S., Cowman, Alan F.

Erythrocyte invasion by Plasmodium requires molecules present both on the merozoite surface and within the specialized organelles of the apical complex. The Plasmodium erythrocyte binding protein...

clag9: A cytoadherence gene in Plasmodium falciparum essential for binding of parasitized erythrocytes to CD36

Trenholme, Katharine R., Gardiner, Donald L., Holt, Deborah C., Thomas, Elizabeth A., Cowman, Alan F., Kemp, David J.

The propensity of isolates of the malaria parasite Plasmodium falciparum to delete a segment of chromosome 9 has provided positional information that has allowed us to identify a gene necessary for...

The adhesion of Plasmodium falciparum-infected erythrocytes to chondroitin sulfate A is mediated by P. falciparum erythrocyte membrane protein 1

Reeder, John C., Cowman, Alan F., Davern, Kathleen M., Beeson, James G., Thompson, Jennifer K., Rogerson, Stephen J., ...

Chondroitin sulfate A (CSA) is an important receptor for the sequestration of Plasmodium falciparum in the placenta, but the parasite ligand involved in adhesion has not previously been identified....

Nuclear-encoded proteins target to the plastid in Toxoplasma gondii and Plasmodium falciparum

Waller, Ross F., Keeling, Patrick J., Donald, Robert G. K., Striepen, Boris, Handman, Emanuela, Lang-Unnasch, Naomi, ...

A vestigial, nonphotosynthetic plastid has been identified recently in protozoan parasites of the phylum Apicomplexa. The apicomplexan plastid, or “apicoplast,” is indispensable, but the complete...

Mutations in dihydropteroate synthase are responsible for sulfone and sulfonamide resistance in Plasmodium falciparum

Triglia, Tony, Menting, John G. T., Wilson, Craig, Cowman, Alan F.

Plasmodium falciparum causes the most severe form of malaria in humans. An important class of drugs in malaria treatment is the sulfone/sulfonamide group, of which sulfadoxine is the most commonly...

An alteration in concatameric structure is associated with efficient segregation of plasmids in transfected Plasmodium falciparum parasites

O’Donnell, Rebecca A., Preiser, Peter R., Williamson, Donald H., Moore, Peter W., Cowman, Alan F., Crabb, Brendan S.

Transfection of the human malaria parasite Plasmodium falciparum is currently performed with circularised plasmids that are maintained episomally in parasites under drug selection but which are...

Identification of Proteins from Plasmodium falciparum That Are Homologous to Reticulocyte Binding Proteins in Plasmodium vivax

Triglia, Tony, Thompson, Jenny, Caruana, Sonia R., Delorenzi, Mauro, Speed, Terry, Cowman, Alan F.

Plasmodium falciparum infections can be fatal, while P. vivax infections usually are not. A possible factor involved in the greater virulence of P. falciparum is that this parasite grows in red blood...

Plasmodium falciparum Homologue of the Genes for Plasmodium vivax and Plasmodium yoelii Adhesive Proteins, Which Is Transcribed but Not Translated

Taylor, Helen M., Triglia, Tony, Thompson, Jenny, Sajid, Mohammed, Fowler, Ruth, Wickham, Mark E., ...

The 235-kDa family of rhoptry proteins in Plasmodium yoelii and the two reticulocyte binding proteins of P. vivax comprise a family of proteins involved in host cell selection and erythrocyte...

Trafficking and assembly of the cytoadherence complex in Plasmodium falciparum-infected human erythrocytes

Wickham, Mark E., Rug, Melanie, Ralph, Stuart A., Klonis, Nectarios, McFadden, Geoffrey I., Tilley, Leann, ...

After invading human erythrocytes, the malarial parasite Plasmodium falciparum, initiates a remarkable process of secreting proteins into the surrounding erythrocyte cytoplasm and plasma membrane....

A genetic screen for improved plasmid segregation reveals a role for Rep20 in the interaction of Plasmodium falciparum chromosomes

O’Donnell, Rebecca A., Freitas-Junior, Lúcio H., Preiser, Peter R., Williamson, Donald H., Duraisingh, Manoj, McElwain, Terry F., ...

Bacterial plasmids introduced into the human malaria parasite Plasmodium falciparum replicate well but are poorly segregated during mitosis. In this paper, we screened a random P.falciparum genomic...

Identification and Disruption of the Gene Encoding the Third Member of the Low-Molecular-Mass Rhoptry Complex in Plasmodium falciparum

Baldi, Deborah L., Good, Robert, Duraisingh, Manoj T., Crabb, Brendan S., Cowman, Alan F.

The low-molecular-mass rhoptry complex of Plasmodium falciparum consists of three proteins, rhoptry-associated protein 1 (RAP1), RAP2, and RAP3. The genes encoding RAP1 and RAP2 are known; however,...

Independent Translocation of Two Micronemal Proteins in Developing Plasmodium falciparum Merozoites

Healer, Julie, Crawford, Simon, Ralph, Stuart, McFadden, Geoff, Cowman, Alan F.

Apical membrane antigen 1 of Plasmodium falciparum (PfAMA1) contains an N-terminal propeptide that is removed prior to the translocation of the mature protein onto the merozoite surface. We localized...

A Type II Pathway for Fatty Acid Biosynthesis Presents Drug Targets in Plasmodium falciparum

Waller, Ross F., Ralph, Stuart A., Reed, Michael B., Su, Vanessa, Douglas, James D., Minnikin, David E., ...

It has long been held that the malaria parasite, Plasmodium sp., is incapable of de novo fatty acid synthesis. This view has recently been overturned with the emergence of data for the presence of a...

Phenotypic variation of Plasmodium falciparum merozoite proteins directs receptor targeting for invasion of human erythrocytes

Duraisingh, Manoj T., Triglia, Tony, Ralph, Stuart A., Rayner, Julian C., Barnwell, John W., McFadden, Geoffrey I., ...

The members of the phylum Apicomplexa parasitize a wide range of eukaryotic host cells. Plasmodium falciparum, responsible for the most virulent form of malaria, invades human erythrocytes using...

Erythrocyte-binding antigen 175 mediates invasion in Plasmodium falciparum utilizing sialic acid-dependent and -independent pathways

Duraisingh, Manoj T., Maier, Alexander G., Triglia, Tony, Cowman, Alan F.

The Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175) is a ligand for merozoite invasion into human erythrocytes that binds to glycophorin A in a sialic acid-dependent manner. P....

Targeted mutagenesis of Plasmodium falciparum erythrocyte membrane protein 3 (PfEMP3) disrupts cytoadherence of malaria-infected red blood cells

Waterkeyn, Jacqueline G., Wickham, Mark E., Davern, Kathleen M., Cooke, Brian M., Coppel, Ross L., Reeder, John C., ...

Adhesion of parasite-infected red blood cells to the vascular endothelium is a critical event in the pathogenesis of malaria caused by Plasmodium falciparum. Adherence is mediated by the variant...

RAP1 controls rhoptry targeting of RAP2 in the malaria parasite Plasmodium falciparum

Baldi, Deborah L., Andrews, Katherine T., Waller, Ross F., Roos, David S., Howard, Randall F., Crabb, Brendan S., ...

Rhoptry associated protein 1 (RAP1) and 2 (RAP2), together with a poorly described third protein RAP3, form the low molecular weight complex within the rhoptries of Plasmodium falciparum. These...

Plasmodium falciparum virulence determinants unveiled

Crabb, Brendan S, Cowman, Alan F

The human malaria parasite Plasmodium falciparum, has many sequences and genes that play key roles in pathogenesis and immune evasion. We must understand the functions of these elements if the...

Protein trafficking to the plastid of Plasmodium falciparum is via the secretory pathway

Waller, Ross F., Reed, Michael B., Cowman, Alan F., McFadden, Geoffrey I.

The plastid of Plasmodium falciparum (or ‘apicoplast’) is the evolutionary homolog of the plant chloroplast and represents a vestige of a photosynthetic past. Apicoplast indispensability...

Plasmodium falciparum Merozoite Surface Protein 6 Is a Dimorphic Antigen

Pearce, J. Andrew, Triglia, Tony, Hodder, Anthony N., Jackson, David C., Cowman, Alan F., Anders, Robin F.

Merozoite surface protein 1 (MSP1) is a highly polymorphic Plasmodium falciparum merozoite surface protein implicated in the invasion of human erythrocytes during the asexual cycle. It forms a...

Correct Promoter Control Is Needed for Trafficking of the Ring-Infected Erythrocyte Surface Antigen to the Host Cytosol in Transfected Malaria Parasites

Rug, Melanie, Wickham, Mark E., Foley, Michael, Cowman, Alan F., Tilley, Leann

Following invasion of human erythrocytes, the malaria parasite, Plasmodium falciparum, exports proteins beyond the confines of its own plasma membrane to modify the properties of the host red cell...

Functional Analysis of Plasmodium falciparum Apical Membrane Antigen 1 Utilizing Interspecies Domains

Healer, Julie, Triglia, Tony, Hodder, Anthony N., Gemmill, Alan W., Cowman, Alan F.

Plasmodium falciparum apical membrane antigen 1 (AMA1) is a leading malaria vaccine candidate whose function has not been unequivocally defined. Partial complementation of function can be achieved by...

Invasion by P. falciparum Merozoites Suggests a Hierarchy of Molecular Interactions

Baum, Jake, Maier, Alexander G, Good, Robert T, Simpson, Ken M, Cowman, Alan F

Central to the pathology of malaria disease are the repeated cycles of parasite invasion and destruction of human erythrocytes. In Plasmodium falciparum, the most virulent species causing malaria,...

Lineage-specific expansion of proteins exported to erythrocytes in malaria parasites

Sargeant, Tobias J, Marti, Matthias, Caler, Elisabet, Carlton, Jane M, Simpson, Ken, Speed, Terence P, ...

A new software was used to predict exported proteins that are conserved between malaria parasites infecting rodents and those infecting humans, revealing a lineage-specific expansion of exported...

Delivery of the Malaria Virulence Protein PfEMP1 to the Erythrocyte Surface Requires Cholesterol-Rich Domains

Frankland, Sarah, Adisa, Akinola, Horrocks, Paul, Taraschi, Theodore F., Schneider, Timothy, Elliott, Salenna R., ...

The particular virulence of the human malaria parasite Plasmodium falciparum derives from export of parasite-encoded proteins to the surface of the mature erythrocytes in which it resides. The...

Genesis of and Trafficking to the Maurer's Clefts of Plasmodium falciparum-Infected Erythrocytes†

Spycher, Cornelia, Rug, Melanie, Klonis, Nectarios, Ferguson, David J. P., Cowman, Alan F., Beck, Hans-Peter, ...

Malaria parasites export proteins beyond their own plasma membrane to locations in the red blood cells in which they reside. Maurer's clefts are parasite-derived structures within the host cell...

Functional analysis of Plasmodium falciparum merozoite antigens: implications for erythrocyte invasion and vaccine development.

Cowman, Alan F, Baldi, Deborah L, Duraisingh, Manoj, Healer, Julie, Mills, Kerry E, O'Donnell, Rebecca A, ...

Malaria is a major human health problem and is responsible for over 2 million deaths per year. It is caused by a number of species of the genus Plasmodium, and Plasmodium falciparum is the causative...

Trafficking of the major virulence factor to the surface of transfected P falciparum–infected erythrocytes

Knuepfer, Ellen, Rug, Melanie, Klonis, Nectarios, Tilley, Leann, Cowman, Alan F.

After invading human red blood cells (RBCs) the malaria parasite Plasmodium falciparum remodels the host cell by trafficking proteins to the RBC compartment. The virulence protein P falciparum...

The role of KAHRP domains in knob formation and cytoadherence of P falciparum-infected human erythrocytes

Rug, Melanie, Prescott, Stuart W., Fernandez, Kate M., Cooke, Brian M., Cowman, Alan F.

Surface protrusions of Plasmodium falciparum-infected erythrocytes, called knobs, display focal aggregates of P falciparum erythrocyte membrane protein 1 (PfEMP1), the adhesion ligand binding...

Inhibition of Dendritic Cell Maturation by Malaria Is Dose Dependent and Does Not Require Plasmodium falciparum Erythrocyte Membrane Protein 1▿

Elliott, Salenna R., Spurck, Timothy P., Dodin, Joelle M., Maier, Alexander G., Voss, Till S., Yosaatmadja, Francisca, ...

Red blood cells infected with Plasmodium falciparum (iRBCs) have been shown to modulate maturation of human monocyte-derived dendritic cells (DCs), interfering with their ability to activate T cells....

Skeleton-binding protein 1 functions at the parasitophorous vacuole membrane to traffic PfEMP1 to the Plasmodium falciparum–infected erythrocyte surface

Maier, Alexander G., Rug, Melanie, O'Neill, Matthew T., Beeson, James G., Marti, Matthias, Reeder, John, ...

A key feature of Plasmodium falciparum, the parasite causing the most severe form of malaria in humans, is its ability to export parasite molecules onto the surface of the erythrocyte. The major...

Variation in use of erythrocyte invasion pathways by Plasmodium falciparum mediates evasion of human inhibitory antibodies

Persson, Kristina E.M., McCallum, Fiona J., Reiling, Linda, Lister, Nicole A., Stubbs, Janine, Cowman, Alan F., ...

Antibodies that inhibit Plasmodium falciparum invasion of erythrocytes are believed to be an important component of immunity against malaria. During blood-stage infection, P. falciparum can use...

Alterations in local chromatin environment are involved in silencing and activation of subtelomeric var genes in Plasmodium falciparum

Voss, Till S, Tonkin, Christopher J, Marty, Allison J, Thompson, Jennifer K, Healer, Julie, Crabb, Brendan S, ...

Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), encoded by the var gene family, undergoes antigenic variation and plays an important role in chronic infection and severe malaria. Only...

Signal-mediated export of proteins from the malaria parasite to the host erythrocyte

Marti, Matthias, Baum, Jake, Rug, Melanie, Tilley, Leann, Cowman, Alan F.

Intracellular parasites from the genus Plasmodium reside and multiply in a variety of cells during their development. After invasion of human erythrocytes, asexual stages from the most virulent...

Antibodies against Merozoite Surface Protein (Msp)-119 Are a Major Component of the Invasion-Inhibitory Response in Individuals Immune to Malaria

O'Donnell, Rebecca A., Burt, Rachel A., Bockarie, Moses, Reeder, John C., Cowman, Alan F., ...

Antibodies that bind to antigens expressed on the merozoite form of the malaria parasite can inhibit parasite growth by preventing merozoite invasion of red blood cells. Inhibitory antibodies are...

The cytoplasmic domain of the Plasmodium falciparum ligand EBA-175 is essential for invasion but not protein trafficking

Gilberger, Tim-Wolf, Thompson, Jennifer K., Reed, Michael B., Good, Robert T., Cowman, Alan F.

The invasion of host cells by the malaria parasite Plasmodium falciparum requires specific protein–protein interactions between parasite and host receptors and an intracellular translocation...

Characterization of a Conserved Rhoptry-Associated Leucine Zipper-Like Protein in the Malaria Parasite Plasmodium falciparum▿†

Haase, Silvia, Cabrera, Ana, Langer, Christine, Treeck, Moritz, Struck, Nicole, Herrmann, Susann, ...

One of the key processes in the pathobiology of the malaria parasite is the invasion and subsequent modification of the human erythrocyte. In this complex process, an unknown number of parasite...

Evolution of malaria parasite plastid targeting sequences

Tonkin, Christopher J., Foth, Bernardo J., Ralph, Stuart A., Struck, Nicole, Cowman, Alan F., McFadden, Geoffrey I.

The transfer of genes from an endosymbiont to its host typically requires acquisition of targeting signals by the gene product to ensure its return to the endosymbiont for function. Many hundreds of...

Exported Proteins Required for Virulence and Rigidity of Plasmodium falciparum-Infected Human Erythrocytes

Maier, Alexander G., Rug, Melanie, O'Neill, Matthew T., Brown, Monica, Chakravorty, Srabasti, Szestak, Tadge, ...

A major part of virulence for Plasmodium falciparum malaria infection, the most lethal parasitic disease of humans, results from increased rigidity and adhesiveness of infected host red cells. These...

Identification of Rhoptry Trafficking Determinants and Evidence for a Novel Sorting Mechanism in the Malaria Parasite Plasmodium falciparum

Richard, Dave, Kats, Lev M., Langer, Christine, Black, Casilda G., Mitri, Khosse, Boddey, Justin A., ...

The rhoptry of the malaria parasite Plasmodium falciparum is an unusual secretory organelle that is thought to be related to secretory lysosomes in higher eukaryotes. Rhoptries contain an extensive...

Sir2 Paralogues Cooperate to Regulate Virulence Genes and Antigenic Variation in Plasmodium falciparum

Tonkin, Christopher J, Carret, Céline K, Duraisingh, Manoj T, Voss, Till S, Ralph, Stuart A, Hommel, Mirja, ...

Cytoadherance of Plasmodium falciparum-infected erythrocytes in the brain, organs and peripheral microvasculature is linked to morbidity and mortality associated with severe malaria. Parasite-derived...

Analysis of structure and function of the giant protein Pf332 in Plasmodium falciparum

Hodder, Anthony N, Maier, Alexander G, Rug, Melanie, Brown, Monica, Hommel, Mirja, Pantic, Ivan, ...

Virulence of Plasmodium falciparum, the most lethal parasitic disease in humans, results in part from adhesiveness and increased rigidity of infected erythrocytes. Pf332 is trafficked to the...

Role of the Plasmodium Export Element in Trafficking Parasite Proteins to the Infected Erythrocyte

Boddey, Justin A, Moritz, Robert L, Simpson, Richard J, Cowman, Alan F

The intracellular survival of Plasmodium falciparum within human erythrocytes is dependent on export of parasite proteins that remodel the host cell. Most exported proteins require a conserved motif...

Type II fatty acid synthesis is essential only for malaria parasite late liver stage development

Vaughan, Ashley M, O'Neill, Matthew T, Tarun, Alice S, Camargo, Nelly, Phuong, Thuan M, Aly, Ahmed S I, ...

Intracellular malaria parasites require lipids for growth and replication. They possess a prokaryotic type II fatty acid synthesis (FAS II) pathway that localizes to the apicoplast plastid organelle...

Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites

Baum, Jake, Papenfuss, Anthony T., Mair, Gunnar R., Janse, Chris J., Vlachou, Dina, Waters, Andrew P., ...

Techniques for targeted genetic disruption in Plasmodium, the causative agent of malaria, are currently intractable for those genes that are essential for blood stage development. The ability to use...

Preerythrocytic, live-attenuated Plasmodium falciparum vaccine candidates by design

VanBuskirk, Kelley M., O'Neill, Matthew T., De La Vega, Patricia, Maier, Alexander G., Krzych, Urszula, Williams, Jack, ...

Falciparum malaria is initiated when Anopheles mosquitoes transmit the Plasmodium sporozoite stage during a blood meal. Irradiated sporozoites confer sterile protection against subsequent malaria...

Plasmodium falciparum Heterochromatin Protein 1 Marks Genomic Loci Linked to Phenotypic Variation of Exported Virulence Factors

Flueck, Christian, Bartfai, Richard, Volz, Jennifer, Niederwieser, Igor, Salcedo-Amaya, Adriana M., Alako, Blaise T. F., ...

Epigenetic processes are the main conductors of phenotypic variation in eukaryotes. The malaria parasite Plasmodium falciparum employs antigenic variation of the major surface antigen PfEMP1, encoded...

Polymorphisms in Erythrocyte Binding Antigens 140 and 181 Affect Function and Binding but Not Receptor Specificity in Plasmodium falciparum▿ †

Maier, Alexander G., Baum, Jake, Smith, Brian, Conway, David J., Cowman, Alan F.

Invasion of human erythrocytes by the malaria parasite Plasmodium falciparum utilizes multiple ligand-receptor interactions involving erythrocyte receptors and parasite erythrocyte binding proteins...

A Novel Family of Apicomplexan Glideosome-associated Proteins with an Inner Membrane-anchoring Role*

Bullen, Hayley E., Tonkin, Christopher J., O'Donnell, Rebecca A., Tham, Wai-Hong, Papenfuss, Anthony T., Gould, Sven, ...

The phylum Apicomplexa are a group of obligate intracellular parasites responsible for a wide range of important diseases. Central to the lifecycle of these unicellular parasites is their ability to...

Reticulocyte binding protein homologues are key adhesins during erythrocyte invasion by Plasmodium falciparum

Triglia, Tony, Tham, Wai-Hong, Hodder, Anthony, Cowman, Alan F

The Apicomplexan parasite responsible for the most virulent form of malaria, Plasmodium falciparum, invades human erythrocytes through multiple ligand–receptor interactions. The P. falciparum...