Aldo A. Rossini

TLR agonists prevent the establishment of allogeneic hematopoietic chimerism in mice treated with costimulation blockade (2009)

Miller, David M., Thornley, Thomas B., Pearson, Todd, Kruger, Annie Joseph, Yamazaki, Masahiro, Shultz, Leonard D., ...

Activation of TLR4 by administration of LPS shortens the survival of skin allografts in mice treated with costimulation blockade through a CD8 T cell-dependent, MyD88-dependent, and type I IFN...

Depletion of the programmed death-1 receptor completely reverses established clonal anergy in CD4(+) T lymphocytes via an interleukin-2-dependent mechanism (2009)

Bishop, Kenneth D., Harris, John E., Mordes, John P., Greiner, Dale L., Rossini, Aldo A., Czech, Michael P., ...

Recent studies have implicated the cell surface receptor Programmed Death-1 (PD-1) in numerous models of T cell anergy, though the specific mechanisms by which the PD-1 signal maintains tolerance is...

TLR agonists abrogate co-stimulation blockade-induced mixed chimerism and transplantation tolerance. (2008)

Miller, David M., Thornley, Thomas B., Pearson, Todd, Yamazaki, Masahiro, Brehm, Michael A., Rossini, Aldo A., ...

We investigated the mechanisms by which Toll-like receptor (TLR) agonists affect the induction of mixed chimerism and skin allograft survival in mice treated with co-stimulation blockade (CB). We...

Idd loci synergize to prolong islet allograft survival induced by costimulation blockade in NOD mice (2008)

Mangada, Julie A., Pearson, Todd, Brehm, Michael A., Wicker, Linda S., Peterson, Laurence B., Shultz, Leonard D., ...

OBJECTIVE: NOD mice model human type 1 diabetes and are used to investigate tolerance induction protocols for islet transplantation in a setting of autoimmunity. However, costimulation blockade-based...

Non-obese diabetic-recombination activating gene-1 (NOD-Rag1 null) interleukin (IL)-2 receptor common gamma chain (IL2r gamma null) null mice: a radioresistant model for human lymphohaematopoietic engraftment. (2008)

Pearson, Todd, Shultz, Leonard D., Miller, David M., King, Marie A., Laning, Joseph, Fodor, William, ...

Immunodeficient hosts engrafted with human lymphohaematopoietic cells hold great promise as a preclinical bridge for understanding human haematopoiesis and immunity. We now describe a new...

Viral infection: a potent barrier to transplantation tolerance (2008)

Miller, David M., Thornley, Thomas B., Greiner, Dale L., Rossini, Aldo A.

Transplantation of allogeneic organs has proven to be an effective therapeutic for a large variety of disease states, but the chronic immunosuppression that is required for organ allograft survival...

Expanded CD34+ human umbilical cord blood cells generate multiple lymphohematopoietic lineages in NOD-scid IL2rgamma(null) mice (2008)

Giassi, Lisa J., Pearson, Todd, Shultz, Leonard D., Laning, Joseph, Biber, Kristin, Kraus, Morey, ...

Umbilical cord blood (UCB) is increasingly being used for human hematopoietic stem cell (HSC) transplantation in children but often requires pooling multiple cords to obtain sufficient numbers for...

A new immunodeficient hyperglycaemic mouse model based on the Ins2Akita mutation for analyses of human islet and beta stem and progenitor cell function (2008)

Pearson, Todd, Shultz, Leonard D., Lief, J., Burzenski, Lisa M., Gott, Bruce, Chase, T., ...

AIMS/HYPOTHESIS: To develop and validate a new immunodeficient mouse strain that spontaneously develops a non-autoimmune hyperglycaemia to serve as a diabetic host for human islets and human beta...

Protein kinase C signaling during T cell activation induces the endoplasmic reticulum stress response (2008)

Pino, Steven C., O'Sullivan-Murphy, Bryan, Lidstone, Erich A., Thornley, Thomas B., Jurczyk, Agata, Urano, Fumihiko, ...

T cell receptor (TCR) ligation (signal one) in the presence of co-stimulation (signal two) results in downstream signals that increase protein production enabling naive T cells to fully activate and...

Role of innate immunity in transplantation tolerance. (2008)

Miller, David M., Rossini, Aldo A., Greiner, Dale L.

Allogeneic organ transplantation has proven to be an effective therapeutic strategy for patients with end-stage organ disease. However, the chronic immunosuppression that is required for the survival...

Viral Infection: A Potent Barrier to Transplantation Tolerance (2008)

David M. Miller, Thomas B. Thornley, Dale L. Greiner, Aldo A. Rossini

Transplantation of allogeneic organs has proven to be an effective therapeutic for a large variety of disease states, but the chronic immunosuppression that is required for organ allograft survival...

Viral Infection: A Potent Barrier to Transplantation Tolerance (2008)

David M. Miller, Thomas B. Thornley, Dale L. Greiner, Aldo A. Rossini

Transplantation of allogeneic organs has proven to be an effective therapeutic for a large variety of disease states, but the chronic immunosuppression that is required for organ allograft survival...

A new Hu-PBL model for the study of human islet alloreactivity based on NOD-scid mice bearing a targeted mutation in the IL-2 receptor gamma chain gene (2007)

King, Marie A., Pearson, Todd, Shultz, Leonard D., Leif, Jean H., Bottino, Rita, Trucco, Massimo, ...

Immunodeficient NOD-scid mice bearing a targeted mutation in the IL2 receptor common gamma chain (Il2rgamma(null)) readily engraft with human stem cells. Here we analyzed human peripheral blood...

Type 1 IFN mediates cross-talk between innate and adaptive immunity that abrogates transplantation tolerance (2007)

Thornley, Thomas B., Phillips, Nancy E., Beaudette-Zlatanova, Britte C., Markees, Thomas G., Bahl, Kapil, Brehm, Michael A., ...

TLR activation of innate immunity prevents the induction of transplantation tolerance and shortens skin allograft survival in mice treated with costimulation blockade. The mechanism by which TLR...

Different mechanisms control peripheral and central tolerance in hematopoietic chimeric mice (2007)

Yamazaki, Masahiro, Pearson, Todd, Brehm, Michael A., Miller, David M., Mangada, Julie A., Markees, Thomas G., ...

Regulatory T cells (Treg) are important in peripheral tolerance, but their role in establishing and maintaining hematopoietic mixed chimerism and generating central tolerance is unclear. We now show...

Development of new-generation HU-PBMC-NOD/SCID mice to study human islet alloreactivity (2007)

King, Marie A., Pearson, Todd, Shultz, Leonard D., Leif, Jean H., Bottino, Rita, Trucco, Massimo, ...

The use of "humanized" mice represents an appealing translational model for studies of the pathogenesis of immune-mediated diseases and for the evaluation of potential therapeutics. The utility of...

Development of new-generation HU-PBMC-NOD/SCID mice to study human islet alloreactivity (2007)

King, Marie A., Pearson, Todd, Shultz, Leonard D., Leif, Jean, Bottino, Rita, Trucco, Massimo, ...

The use of "humanized" mice represents an appealing translational model for studies of the pathogenesis of immune-mediated diseases and for the evaluation of potential therapeutics. The utility of...

Humanized NOD/LtSz-scid IL2 receptor common gamma chain knockout mice in diabetes research (2007)

Shultz, Leonard D., Pearson, Todd, King, Marie A., Giassi, Lisa J., Carney, Lisa, Gott, Bruce, ...

There are many rodent models of autoimmune diabetes that have been used to study the pathogenesis of human type 1 diabetes (T1D), including the non-obese diabetic (NOD) mouse, the biobreeding (BB)...

Passing the baton--to whom (2007)

Rossini, Aldo A.

Scientific discovery occasionally occurs as a sudden and dramatic leap ahead but more often proceeds at a subtler and steadier pace. Each small step forward may escape public notice but is ultimately...

TLR9-signaling pathways are involved in Kilham rat virus-induced autoimmune diabetes in the biobreeding diabetes-resistant rat (2007)

Zipris, Danny, Lien, Egil, Nair, Anjali, Xie, Jenny X., Greiner, Dale L., Mordes, John P., ...

Viral infections are associated epidemiologically with the expression of type 1 diabetes in humans, but the mechanisms underlying this putative association are unknown. To investigate the role of...

A regulatory CD4+ T cell subset in the BB rat model of autoimmune diabetes expresses neither CD25 nor Foxp3 (2006)

Hillebrands, Jan-Luuk, Whalen, Barbara J., Visser, Jeroen T. J., Koning, Jasper, Bishop, Kenneth D., Leif, Jean, ...

Biobreeding (BB) rats model type 1 autoimmune diabetes (T1D). BB diabetes-prone (BBDP) rats develop T1D spontaneously. BB diabetes-resistant (BBDR) rats develop T1D after immunological perturbations...

Rapid quantification of naive alloreactive T cells by TNF-alpha production and correlation with allograft rejection in mice (2006)

Brehm, Michael A., Mangada, Julie A., Markees, Thomas G., Pearson, Todd, Daniels, Keith A., Thornley, Thomas B., ...

Allograft transplantation requires chronic immunosuppression, but there is no effective strategy to evaluate the long-term maintenance of immunosuppression other than assessment of graft function....

Regulation of insulin biosynthesis in pancreatic beta cells by an endoplasmic reticulum-resident protein kinase IRE1 (2006)

Lipson, Kathryn L., Fonseca, Sonya G., Ishigaki, Shinsuke, Nguyen, Linh X., Foss, Elizabeth, Bortell, Rita, ...

In pancreatic beta cells, the endoplasmic reticulum (ER) is an important site for insulin biosynthesis and the folding of newly synthesized proinsulin. Here, we show that IRE1alpha, an ER-resident...

ART2, a T cell surface mono-ADP-ribosyltransferase, generates extracellular poly(ADP-ribose) (2006)

Morrison, Alan R., Moss, Joel, Stevens, Linda A., Evans, James E., Farrell, Caitlin, Merithew, Eric Lee, ...

NAD functions in multiple aspects of cellular metabolism and signaling through enzymes that covalently transfer ADP-ribose from NAD to acceptor proteins, thereby altering their function. NAD is a...

TLR agonists abrogate costimulation blockade-induced prolongation of skin allografts (2006)

Thornley, Thomas B., Brehm, Michael A., Markees, Thomas G., Shultz, Leonard D., Mordes, John P., Welsh, Raymond M., ...

Costimulation blockade protocols are effective in prolonging allograft survival in animal models and are entering clinical trials, but how environmental perturbants affect graft survival remains...

Disruption of the Jnk2 (Mapk9) gene reduces destructive insulitis and diabetes in a mouse model of type I diabetes (2005)

Jaeschke, Anja, Rincon, Mercedes, Doran, Beth, Reilly, Judith, Neuberg, Donna S., Greiner, Dale L., ...

The c-Jun NH(2)-terminal kinase isoform (JNK) 1 is implicated in type 2 diabetes. However, a potential role for the JNK2 protein kinase in diabetes has not been established. Here, we demonstrate that...

Autoimmune diabetes and resistance to xenograft transplantation tolerance in NOD mice (2004)

Gordon, Ethel J., Wicker, Linda S., Peterson, Laurence B., Serreze, David V., Markees, Thomas G., Shultz, Leonard D., ...

Costimulation blockade induces prolonged rat islet and skin xenograft survival in C57BL/6 mice. Nonobese diabetic (NOD) mice, which are used to model human autoimmune diabetes, are resistant to...

TLR activation synergizes with Kilham rat virus infection to induce diabetes in BBDR rats (2004)

Zipris, Danny, Lien, Egil, Xie, Jenny X., Greiner, Dale L., Mordes, John P., Rossini, Aldo A.

Virus infection is hypothesized to be an important environmental "trigger" of type 1 diabetes in humans. We used the BBDR rat model to investigate the relationship between viral infection and...

Evaluation of donor-specific transfusion sources: unique failure of bone marrow cells to induce prolonged skin allograft survival with anti-CD154 monoclonal antibody (2004)

Markees, Thomas G., Pearson, Todd, Cuthbert, Amy, Pearson, Andrea L., Shultz, Leonard D., Leif, Jean H., ...

BACKGROUND: Treatment with anti-CD154 monoclonal antibody (mAb) plus a donor-specific transfusion (DST) of spleen cells prolongs skin allograft survival in mice through a mechanism involving deletion...

Early growth response gene-2, a zinc-finger transcription factor, is required for full induction of clonal anergy in CD4+ T cells (2004)

Harris, John E., Bishop, Kenneth D., Phillips, Nancy E., Mordes, John P., Greiner, Dale L., Rossini, Aldo A., ...

Ag-specific immune tolerance results from the induction of cellular mechanisms that limit T cell responses to selective Ags. One of these mechanisms is characterized by attenuated proliferation and...

Islet allograft survival induced by costimulation blockade in NOD mice is controlled by allelic variants of Idd3 (2004)

Pearson, Todd, Weiser, Peter, Markees, Thomas G., Serreze, David V., Wicker, Linda S., Peterson, Laurence B., ...

NOD mice develop type 1 autoimmune diabetes and exhibit genetically dominant resistance to transplantation tolerance induction. These two phenotypes are genetically separable. Costimulation blockade...

Deficiencies in gut NK cell number and function precede diabetes onset in BB rats (2004)

Todd, Derrick James, Forsberg, Eric M., Greiner, Dale L., Mordes, John P., Rossini, Aldo A., Bortell, Rita

Defects in the intestinal immune system may contribute to the pathogenesis of autoimmune diseases. Intraepithelial lymphocytes represent a substantial fraction of gut-associated lymphocytes, but...

Autoimmune diabetes and the circle of tolerance (2004)

Rossini, Aldo A.

The concept of immunological tolerance is central to our understanding of type 1 diabetes and the development of strategies for its prediction, prevention, and cure. Tolerance simply refers to the...

Islet cell autoimmunity and transplantation tolerance: two distinct mechanisms (2003)

Pearson, Todd, Markees, Thomas G., Serreze, David V., Pierce, Melissa A., Wicker, Linda S., Peterson, Laurence B., ...

Recent advances in islet transplantation have enabled physicians to cure type 1 autoimmune diabetes, but at the cost of lifelong immunosuppression with its attendant side effects and long-term health...

Alloimmune injury and rejection of human skin grafts on human peripheral blood lymphocyte-reconstituted non-obese diabetic severe combined immunodeficient beta2-microglobulin-null mice (2003)

Turgeon, Nicole A., Banuelos, Scott J., Shultz, Leonard D., Lyons, Bonnie L., Iwakoshi, Neal N., Greiner, Dale L., ...

Small animal models with the capacity to support engraftment of a functional human immune system are needed to facilitate studies of human alloimmunity. In the present investigation, non-obese...

Hematopoietic chimerism and central tolerance created by peripheral-tolerance induction without myeloablative conditioning (2003)

Seung, Edward, Mordes, John P., Rossini, Aldo A., Greiner, Dale L.

Allogeneic hematopoietic chimerism leading to central tolerance has significant therapeutic potential. Realization of that potential has been impeded by the need for myeloablative conditioning of the...

Ian4 is required for mitochondrial integrity and T cell survival (2003)

Pandarpurkar, Malini, Wilson-Fritch, Leanne, Corvera, Silvia, Markholst, Helle, Hornum, Lars, Greiner, Dale L., ...

Apoptosis is a regulated cell death program controlled by extrinsic and intrinsic signaling pathways. The intrinsic pathway involves stress signals that activate pro-apoptotic members of the Bcl-2...

Induction of tolerance for islet transplantation for type 1 diabetes (2003)

Seung, Edward, Mordes, John P., Greiner, Dale L., Rossini, Aldo A.

Type 1 diabetes is an autoimmune disorder characterized by selective destruction of pancreatic b cells and absolute insulin deficiency. Even when treated well, control is imperfect and complications...

Genetic disassociation of autoimmunity and resistance to costimulation blockade-induced transplantation tolerance in nonobese diabetic mice (2003)

Pearson, Todd, Markees, Thomas G., Serreze, David V., Pierce, Melissa A., Marron, Michele P., Wicker, Linda S., ...

Curing type 1 diabetes by islet transplantation requires overcoming both allorejection and recurrent autoimmunity. This has been achieved with systemic immunosuppression, but tolerance induction...

Direct visualization of cross-reactive effector and memory allo-specific CD8 T cells generated in response to viral infections (2003)

Brehm, Michael A., Markees, Thomas G., Daniels, Keith A., Greiner, Dale L., Rossini, Aldo A., Welsh, Raymond M.

CD8 T cell cross-reactivity between heterologous viruses has been shown to provide protective immunity, induce immunopathology, influence the immunodominance of epitope-specific T cell responses, and...

Infections that induce autoimmune diabetes in BBDR rats modulate CD4+CD25+ T cell populations (2003)

Zipris, Danny, Hillebrands, Jan-Luuk, Welsh, Raymond M., Rozing, Jan, Xie, Jenny X., Mordes, John P., ...

Viruses are believed to contribute to the pathogenesis of autoimmune type 1A diabetes in humans. This pathogenic process can be modeled in the BBDR rat, which develops pancreatic insulitis and type...

Blockade of CD40-mediated signaling is sufficient for inducing islet but not skin transplantation tolerance (2003)

Phillips, Nancy E., Markees, Thomas G., Mordes, John P., Greiner, Dale L., Rossini, Aldo A.

Treatment of mice with a single donor-specific transfusion (DST) plus a brief course of anti-CD154 mAb to block CD40-mediated signaling uniformly induces donor-specific transplantation tolerance....

NOD congenic mice genetically protected from autoimmune diabetes remain resistant to transplantation tolerance induction (2003)

Pearson, Todd, Markees, Thomas G., Wicker, Linda S., Serreze, David V., Peterson, Laurence B., Mordes, John P., ...

The loss of self-tolerance leading to autoimmune type 1 diabetes in the NOD mouse model involves at least 19 genetic loci. In addition to their genetic defects in self-tolerance, NOD mice resist...

Viral abrogation of stem cell transplantation tolerance causes graft rejection and host death by different mechanisms (2002)

Forman, Daron, Welsh, Raymond M., Markees, Thomas G., Woda, Bruce A., Mordes, John P., Rossini, Aldo A., ...

Tolerance-based stem cell transplantation using sublethal conditioning is being considered for the treatment of human disease, but safety and efficacy remain to be established. We have shown that...

Skin allograft maintenance in a new synchimeric model system of tolerance (2001)

Iwakoshi, Neal N., Markees, Thomas G., Turgeon, Nicole A., Thornley, Thomas B., Cuthbert, Amy, Leif, Jean, ...

Treatment of mice with a single donor-specific transfusion plus a brief course of anti-CD154 mAb uniformly induces donor-specific transplantation tolerance characterized by the deletion of...

Levels of Art2+ cells but not soluble Art2 protein correlate with expression of autoimmune diabetes in the BB rat (2001)

Bortell, Rita, Waite, Debra J., Whalen, Barbara J., Todd, Derrick James, Leif, Jean H., Lesma, Elena, ...

ART2a and ART2b are isoenzymes expressed on the surface of mature T cells and intraepithelial lymphocytes (IELs) in the rat. They exhibit both adenosine diphosphoribosyltransferase and nicotine...

An atypical population of NK cells that spontaneously secrete IFN-gamma and IL-4 is present in the intraepithelial lymphoid compartment of the rat (2001)

Todd, Derrick James, Greiner, Dale L., Rossini, Aldo A., Mordes, John P., Bortell, Rita

The intestinal lymphoid compartment of the rat is large and diverse, but the phenotype and functions of its constituent cell populations are not fully characterized. Using new methodology for the...

Requirement of the JIP1 scaffold protein for stress-induced JNK activation (2001)

Whitmarsh, Alan J., Kuan, Chia-Yi, Kennedy, Norman J., Kelkar, Nyaya, Haydar, Tarik F., Mordes, John P., ...

The c-Jun N-terminal kinase (JNK) signal transduction pathway is activated in response to the exposure of cells to environmental stress. Components of the JNK signaling pathway interact with the JIP1...

Nicotinamide adenine dinucleotide (NAD) and its metabolites inhibit T lymphocyte proliferation: role of cell surface NAD glycohydrolase and pyrophosphatase activities (2001)

Bortell, Rita, Moss, Joel, McKenna, Robert C., Rigby, Mark R., Niedzwiecki, Dena, Stevens, Linda A., ...

The presence of NAD-metabolizing enzymes (e.g., ADP-ribosyltransferase (ART)2) on the surface of immune cells suggests a potential immunomodulatory activity for ecto-NAD or its metabolites at sites...

BB rat thymocytes cultured in the presence of islets lose their ability to transfer autoimmune diabetes (2001)

Whalen, Barbara J., Marounek, Jan, Weiser, Peter, Appel, Michael C., Greiner, Dale L., Mordes, John P., ...

Thymocytes from adult BB rats can adoptively transfer autoimmune diabetes to athymic recipients. It is also known that the development of BB rat T-cells is recapitulated in adult thymus organ...

Viral infection abrogates CD8(+) T-cell deletion induced by costimulation blockade (2000)

Turgeon, Nicole A., Iwakoshi, Neal N., Phillips, Nancy E., Meyers, William C., Welsh, Raymond M., Greiner, Dale L., ...

BACKGROUND: Treatment with a single donor-specific transfusion (DST) plus a brief course of anti-CD154 monoclonal antibody (mAb) prolongs skin allograft survival in mice. It is known that...

Virus-induced abrogation of transplantation tolerance induced by donor-specific transfusion and anti-CD154 antibody (2000)

Welsh, Raymond M., Markees, Thomas G., Woda, Bruce A., Daniels, Keith A., Brehm, Michael A., Mordes, John P., ...

Treatment with a 2-week course of anti-CD154 antibody and a single transfusion of donor leukocytes (a donor-specific transfusion or DST) permits skin allografts to survive for >100 days in...

Treatment of allograft recipients with donor-specific transfusion and anti-CD154 antibody leads to deletion of alloreactive CD8+ T cells and prolonged graft survival in a CTLA4-dependent manner (1999)

Iwakoshi, Neal N., Mordes, John P., Markees, Thomas G., Phillips, Nancy E., Rossini, Aldo A., Greiner, Dale L.

A two-element protocol consisting of one donor-specific transfusion (DST) plus a brief course of anti-CD154 mAb greatly prolongs the survival of murine islet, skin, and cardiac allografts. To study...

Diabetes prone BB rats are severely deficient in natural killer T cells (1999)

Iwakoshi, Neal N., Greiner, Dale L., Rossini, Aldo A., Mordes, John P.

Diabetes prone (DP) BB rats develop spontaneous autoimmune hyperglycemia. Coisogenic diabetes resistant (DR) BB rats develop diabetes in response to immunological and environmental perturbants, but...

A new isolation method for rat intraepithelial lymphocytes (1999)

Todd, Derrick James, Singh, Amrik J., Greiner, Dale L., Mordes, John P., Rossini, Aldo A., Bortell, Rita

Intraepithelial lymphocytes (IELs) play critical roles in gut immunity. In mice, gammadelta T cells are a large component of the IEL population. In the rat, gammadelta IELs are reportedly much less...

NOD mice have a generalized defect in their response to transplantation tolerance induction (1999)

Markees, Thomas G., Serreze, David V., Phillips, Nancy E., Sorli, Christopher H., Gordon, Ethel J., Shultz, Leonard D., ...

A protocol consisting of a single donor-specific transfusion (DST) plus a brief course of anti-CD154 monoclonal antibody (anti-CD40 ligand mAb) induces permanent islet allograft survival in...

Induction of immunologic tolerance for transplantation (1999)

Rossini, Aldo A., Greiner, Dale L., Mordes, John P.

In the second half of the 20th century, the transplantation of replacement organs and tissues to cure disease has become a clinical reality. Success has been achieved as a direct result of progress...

High frequency apoptosis of recent thymic emigrants in the liver of lymphopenic diabetes-prone BioBreeding rats (1998)

Iwakoshi, Neal N., Goldschneider, Irving, Tausche, Frances, Mordes, John P., Rossini, Aldo A., Greiner, Dale L.

Diabetes-prone (DP) BioBreeding (BB) rats develop spontaneous autoimmune diabetes. DP-BB thymocyte export is reduced, and most thymic emigrants disappear rapidly from peripheral lymphoid tissues....

Long-term survival of skin allografts induced by donor splenocytes and anti-CD154 antibody in thymectomized mice requires CD4(+) T cells, interferon-gamma, and CTLA4 (1998)

Markees, Thomas G., Phillips, Nancy E., Gordon, Ethel J., Noelle, Randolph J., Shultz, Leonard D., Mordes, John P., ...

Treatment of C57BL/6 mice with one transfusion of BALB/c spleen cells and anti-CD154 (anti-CD40-ligand) antibody permits BALB/c islet grafts to survive indefinitely and BALB/c skin grafts to survive...

Blockade of CD40-CD154 interferes with human T cell engraftment in scid mice (1998)

Foy, Teresa M., McIlraith, Melissa, Masters, Sally R., Dunn, Jonathan J., Rossini, Aldo A., Shultz, Leonard D., ...

Antibodies to the ligand for CD40 (CD154) have been shown to exert profound effects on the development of cell-mediated immune responses in mice. The present study shows that an antibody to human...

Mouse RT6 locus 1 and rat RT6.2 are NAD+. Arginine ADP-ribosyltransferases with auto-ADP-ribosylation activity (1997)

Bortell, Rita, Rigby, Mark R., Stevens, Linda A., Moss, Joel, Kanaitsuka, Toshihiro, Mordes, John P., ...

We report that rat RT6.2 and recombinant mouse Rt6 locus 1 proteins possess auto-ADP-ribosyltransferase activity and that Rt6, but not RT6, catalyzes the ADP-ribosylation of exogenous histones. Based...

The rat T-cell surface protein RT6 is associated with src family tyrosine kinases and generates an activation signal (1996)

Rigby, Mark R., Bortell, Rita, Greiner, Dale L., Czech, Michael P., Klarlund, Jes K., Mordes, John P., ...

RT6 is a glycosyl-phosphatidylinositol-linked surface molecule present on most mature rat T-cells. RT6+ T-cells can prevent the expression of autoimmune diabetes in the BB rat, but the mechanism is...

Ontogeny and immunohistochemical localization of thymus-dependent and thymus-independent RT6+ cells in the rat (1996)

Waite, Debra J., Appel, Michael C., Handler, Eugene S., Mordes, John P., Rossini, Aldo A., Greiner, Dale L.

RT6 is a cell surface alloantigen that identifies a regulatory subset of peripheral T cells in the rat. Diabetes-prone BB rats are deficient in peripheral RT6+ T cells and develop spontaneous...

Rat RT6.2 and mouse Rt6 locus 1 are NAD+: arginine ADP ribosyltransferases with auto-ADP ribosylation activity (1996)

Rigby, Mark R., Bortell, Rita, Stevens, Linda A., Moss, Joel, Kanaitsuka, Toshihiro, Shigeta, Hirofumi, ...

RT6 is a glycosylphosphatidylinositol-linked protein found on the surface of mature rat T lymphocytes. Cells that express RT6 have an immunoregulatory function and modulate the expression of...

Survival of mouse pancreatic islet allografts in recipients treated with allogeneic small lymphocytes and antibody to CD40 ligand (1995)

Parker, David C., Greiner, Dale L., Phillips, Nancy E., Appel, Michael C., Steele, Alan W., Durie, Fiona H., ...

Combined treatment with allogeneic small lymphocytes or T-depleted small lymphocytes plus a blocking antibody to CD40 ligand (CD40L) permitted indefinite pancreatic islet allograft survival in 37 of...

Thymic epithelial defects and predisposition to autoimmune disease in BB rats (1994)

Doukas, John, Mordes, John P., Swymer, Christine, Niedzwiecki, Dena, Mason, Rebecca, Rozing, Jan, ...

We report an association between thymic epithelial defects and predisposition to autoimmunity. Diabetes-prone (DP) BB rats develop spontaneous hyperglycemia and are deficient in T cell subsets...

High stimulatory activity of dendritic cells from diabetes-prone BioBreeding/Worcester rats exposed to macrophage-derived factors (1993)

Tafuri, Anna, Bowers, William E., Handler, Eugene S., Appel, Michael C., Lew, Robert A., Greiner, Dale L., ...

Dendritic cells (DC) present antigen and initiate T cell-mediated immune responses. To investigate the possible association of autoimmunity with DC function, we compared the accessory activity of...

Immunopathology of diabetes in the RT6-depleted diabetes-resistant BB/Wor rat (1990)

Jiang, Zhong, Handler, Eugene S., Rossini, Aldo A., Woda, Bruce A.

Insulin-dependent diabetes mellitus appears to be an autoimmune disease that is characterized morphologically by insulitis, an inflammation of the pancreatic islets of Langerhans that results in the...

Adoptive transfer of autoimmune diabetes and thyroiditis to athymic rats (1990)

McKeever, Una, Mordes, John P., Greiner, Dale L., Appel, Michael C., Rozing, Jan, Handler, Eugene S., ...

We describe the induction of autoimmune diabetes, insulitis, and thyroiditis in athymic rats following injections of major histocompatibility complex compatible spleen cells. Lymphocytes with these...

Virus-Induced Abrogation of Transplantation Tolerance Induced by Donor-Specific Transfusion and Anti-CD154 Antibody

Welsh, Raymond M., Markees, Thomas G., Woda, Bruce A., Daniels, Keith A., Brehm, Michael A., Mordes, John P., ...

Treatment with a 2-week course of anti-CD154 antibody and a single transfusion of donor leukocytes (a donor-specific transfusion or DST) permits skin allografts to survive for >100 days in...

Hematopoietic chimerism and central tolerance created by peripheral-tolerance induction without myeloablative conditioning

Seung, Edward, Mordes, John P., Rossini, Aldo A., Greiner, Dale L.

Allogeneic hematopoietic chimerism leading to central tolerance has significant therapeutic potential. Realization of that potential has been impeded by the need for myeloablative conditioning of the...

Ian4 is required for mitochondrial integrity and T cell survival

Pandarpurkar, Malini, Wilson-Fritch, Leanne, Corvera, Silvia, Markholst, Helle, Hornum, Lars, Greiner, Dale L., ...

Apoptosis is a regulated cell death program controlled by extrinsic and intrinsic signaling pathways. The intrinsic pathway involves stress signals that activate pro-apoptotic members of the Bcl-2...

Disruption of the Jnk2 (Mapk9) gene reduces destructive insulitis and diabetes in a mouse model of type I diabetes

Jaeschke, Anja, Rincón, Mercedes, Doran, Beth, Reilly, Judith, Neuberg, Donna, Greiner, Dale L., ...

The c-Jun NH2-terminal kinase isoform (JNK) 1 is implicated in type 2 diabetes. However, a potential role for the JNK2 protein kinase in diabetes has not been established. Here, we demonstrate that...

Requirement of the JIP1 scaffold protein for stress-induced JNK activation

Whitmarsh, Alan J., Kuan, Chia-Yi, Kennedy, Norman J., Kelkar, Nyaya, Haydar, Tarik F., Mordes, John P., ...

The c-Jun N-terminal kinase (JNK) signal transduction pathway is activated in response to the exposure of cells to environmental stress. Components of the JNK signaling pathway interact with the JIP1...

Virus-Induced Abrogation of Transplantation Tolerance Induced by Donor-Specific Transfusion and Anti-CD154 Antibody

Welsh, Raymond M., Markees, Thomas G., Woda, Bruce A., Daniels, Keith A., Brehm, Michael A., Mordes, John P., ...

Treatment with a 2-week course of anti-CD154 antibody and a single transfusion of donor leukocytes (a donor-specific transfusion or DST) permits skin allografts to survive for >100 days in...

Hematopoietic chimerism and central tolerance created by peripheral-tolerance induction without myeloablative conditioning

Seung, Edward, Mordes, John P., Rossini, Aldo A., Greiner, Dale L.

Allogeneic hematopoietic chimerism leading to central tolerance has significant therapeutic potential. Realization of that potential has been impeded by the need for myeloablative conditioning of the...

Ian4 is required for mitochondrial integrity and T cell survival

Pandarpurkar, Malini, Wilson-Fritch, Leanne, Corvera, Silvia, Markholst, Helle, Hornum, Lars, Greiner, Dale L., ...

Apoptosis is a regulated cell death program controlled by extrinsic and intrinsic signaling pathways. The intrinsic pathway involves stress signals that activate pro-apoptotic members of the Bcl-2...

Requirement of the JIP1 scaffold protein for stress-induced JNK activation

Whitmarsh, Alan J., Kuan, Chia-Yi, Kennedy, Norman J., Kelkar, Nyaya, Haydar, Tarik F., Mordes, John P., ...

The c-Jun N-terminal kinase (JNK) signal transduction pathway is activated in response to the exposure of cells to environmental stress. Components of the JNK signaling pathway interact with the JIP1...

Disruption of the Jnk2 (Mapk9) gene reduces destructive insulitis and diabetes in a mouse model of type I diabetes

Jaeschke, Anja, Rincón, Mercedes, Doran, Beth, Reilly, Judith, Neuberg, Donna, Greiner, Dale L., ...

The c-Jun NH2-terminal kinase isoform (JNK) 1 is implicated in type 2 diabetes. However, a potential role for the JNK2 protein kinase in diabetes has not been established. Here, we demonstrate that...

Passing the baton — to whom?

Rossini, Aldo A.

Scientific discovery occasionally occurs as a sudden and dramatic leap ahead but more often proceeds at a subtler and steadier pace. Each small step forward may escape public notice but is ultimately...

Rapid quantification of naive alloreactive T cells by TNF-α production and correlation with allograft rejection in mice

Brehm, Michael A., Mangada, Julie, Markees, Thomas G., Pearson, Todd, Daniels, Keith A., Thornley, Thomas B., ...

Allograft transplantation requires chronic immunosuppression, but there is no effective strategy to evaluate the long-term maintenance of immunosuppression other than assessment of graft function....

Viral Infection: A Potent Barrier to Transplantation Tolerance

Miller, David M., Thornley, Thomas B., Greiner, Dale L., Rossini, Aldo A.

Transplantation of allogeneic organs has proven to be an effective therapeutic for a large variety of disease states, but the chronic immunosuppression that is required for organ allograft survival...

CHOP Mediates Endoplasmic Reticulum Stress-Induced Apoptosis in Gimap5-Deficient T Cells

Pino, Steven C., O'Sullivan-Murphy, Bryan, Lidstone, Erich A., Yang, Chaoxing, Lipson, Kathryn L., Jurczyk, Agata, ...

Gimap5 (GTPase of the immunity-associated protein 5) has been linked to the regulation of T cell survival, and polymorphisms in the human GIMAP5 gene associate with autoimmune disorders. The...

Protein kinase C signaling during T cell activation induces the endoplasmic reticulum stress response

Pino, Steven C., O’Sullivan-Murphy, Bryan, Lidstone, Erich A., Thornley, Thomas B., Jurczyk, Agata, Urano, Fumihiko, ...

T cell receptor (TCR) ligation (signal one) in the presence of co-stimulation (signal two) results in downstream signals that increase protein production enabling naïve T cells to fully activate and...