Typescript (photocopy)
Jiang, Peng, Ninfa, Alexander J.
The nitrogen regulator II (NRII or NtrB)-NRI (NtrC) two-component signal transduction system regulates the transcription of nitrogen-regulated genes in Escherichia coli. The NRII protein has both...
Atkinson, Mariette R., Blauwkamp, Timothy A., Bondarenko, Vladamir, Studitsky, Vasily, Ninfa, Alexander J.
The nitrogen-regulated genes and operons of the Ntr regulon of Escherichia coli are activated by the enhancer-binding transcriptional activator NRI∼P (NtrC∼P). Here, we examined the activation of...
Context-Dependent Functions of the PII and GlnK Signal Transduction Proteins in Escherichia coli
Atkinson, Mariette R., Blauwkamp, Timothy A., Ninfa, Alexander J.
Two closely related signal transduction proteins, PII and GlnK, have distinct physiological roles in the regulation of nitrogen assimilation. Here, we examined the physiological roles of PII and GlnK...
Pioszak, Augen A., Ninfa, Alexander J.
Mutant forms of Escherichia coli NRII (NtrB) were isolated that retained wild-type NRII kinase activity but were defective in the PII-activated phosphatase activity of NRII. Mutant strains were...
A protein-induced DNA bend increases the specificity of a prokaryotic enhancer-binding protein
Dworkin, Jonathan, Ninfa, Alexander J., Model, Peter
Control of transcription in prokaryotes often involves direct contact of regulatory proteins with RNA polymerase from binding sites located adjacent to the target promoter. Alternatively, in the case...
Pioszak, Augen A., Ninfa, Alexander J.
The phosphorylated form of NRI is the transcriptional activator of nitrogen-regulated genes in Escherichia coli. NRI∼P displays a slow autophosphatase activity and is rapidly dephosphorylated by...
Jiang, Peng, Ninfa, Alexander J.
The nitrogen regulator II (NRII or NtrB)-NRI (NtrC) two-component signal transduction system regulates the transcription of nitrogen-regulated genes in Escherichia coli. The NRII protein has both...
Atkinson, Mariette R., Blauwkamp, Timothy A., Bondarenko, Vladamir, Studitsky, Vasily, Ninfa, Alexander J.
The nitrogen-regulated genes and operons of the Ntr regulon of Escherichia coli are activated by the enhancer-binding transcriptional activator NRI∼P (NtrC∼P). Here, we examined the activation of...
Context-Dependent Functions of the PII and GlnK Signal Transduction Proteins in Escherichia coli
Atkinson, Mariette R., Blauwkamp, Timothy A., Ninfa, Alexander J.
Two closely related signal transduction proteins, PII and GlnK, have distinct physiological roles in the regulation of nitrogen assimilation. Here, we examined the physiological roles of PII and GlnK...
Pioszak, Augen A., Ninfa, Alexander J.
Mutant forms of Escherichia coli NRII (NtrB) were isolated that retained wild-type NRII kinase activity but were defective in the PII-activated phosphatase activity of NRII. Mutant strains were...
A protein-induced DNA bend increases the specificity of a prokaryotic enhancer-binding protein
Dworkin, Jonathan, Ninfa, Alexander J., Model, Peter
Control of transcription in prokaryotes often involves direct contact of regulatory proteins with RNA polymerase from binding sites located adjacent to the target promoter. Alternatively, in the case...
Pioszak, Augen A., Ninfa, Alexander J.
The phosphorylated form of NRI is the transcriptional activator of nitrogen-regulated genes in Escherichia coli. NRI∼P displays a slow autophosphatase activity and is rapidly dephosphorylated by...
Modular cell biology: retroactivity and insulation
Del Vecchio, Domitilla, Ninfa, Alexander J, Sontag, Eduardo D
Modularity plays a fundamental role in the prediction of the behavior of a system from the behavior of its components, guaranteeing that the properties of individual components do not change upon...
Rate constants rather than biochemical mechanism determine behaviour of genetic clocks
Conrad, Emery, Mayo, Avraham E., Ninfa, Alexander J., Forger, Daniel B.
Many biological systems contain both positive and negative feedbacks. These are often classified as resonators or integrators. Resonators respond preferentially to oscillating signals of a particular...