Alexander Kamb

A simple method for statistical analysis of intensity differences in microarray-derived gene expression data (2001)

Kamb, Alexander, Ramaswami, Mani

Abstract Background Microarray experiments offer a potent solution to the problem of making and comparing large numbers of gene expression measurements either in different cell types or in the same...

Transcriptional transactivation by selected short random peptides attached to lexA-GFP fusion proteins (2001)

Abedi, Majid, Caponigro, Giordano, Shen, Jiaxiang, Hansen, Steven, Sandrock, Tanya, Kamb, Alexander

Abstract Background Transcriptional transactivation is a process with remarkable tolerance for sequence diversity and structural geometry. In studies of the features that constitute transactivating...

Oligonucleotide-conjugated beads for transdominant genetic experiments (2000)

Feldhaus, Michael J., Lualhati, Michael, Cardon, Karen, Roth, Bruce, Kamb, Alexander

Transdominant genetics using expression libraries can identify proteins and peptides that affect cell division. In conjunction with these libraries, oligo­nucleotide-conjugated beads and flow...

Differential Inactivation of CDKN2 and Rb Protein in Non—Small-Cell and Small-Cell Lung Cancer Cell Lines (1995)

Kelley, Michael J., Nakagawa, Kazuhiko, Steinberg, Seth M., Mulshine, James L., Kamb, Alexander, Johnson, Bruce E.

Background: The CDKN2 gene encodes the human cyclin-dependent kinase 4 inhibitor. This inhibitor protein is believed to be a tumor suppressor that plays an essential role in cell cycle regulation....

Comparison of the positional cloning methods used to isolate the BRCA1 gene (1995)

Harshman, Keith, Bell, Russell, Rosenthal, Judith, Katcher, Harold, MlKl, Yoshio, Swenson, Jeff, ...

A critical step in positional cloning is the identification of candidate genes from a large, genetically defined region. Candidate gene isolation by hybrid selection, genomic sequencing, and direct...

Isolation of a diverged homeobox gene, M0X1, from the BRCA1 region on 17q21 by solution hybrid capture (1994)

Futreal, P.Andrew, Cochran, Charles, Rosenthal, Judith, Miki, Yoshlo, Swenson, Jeff, Hobbs, Maurine, ...

Using the technique of solution hybridization coupled with magnetic bead capture, we have isolated a novel homeobox-containing gene from the BRCA1 region of 17q21. This gene is the human homologue of...

Organization of the transcriptional unit of a human class II histocompatibility antigen: HLA-DR heavy chain (1983)

Schamboeck, Anton, Korman, Alan J., Kamb, Alexander, Strominger, Jack L.

A total of 5724 base pairs of a recombinant phage DNA containing a human HLA-DR heavy chain gene including flanking regions has been analyzed. The regions corresponding to all the exons have been...

Transdominant genetic analysis of a growth control pathway

Caponigro, Giordano, Abedi, Majid R., Hurlburt, Anthony P., Maxfield, Andrew, Judd, Weston, Kamb, Alexander

Genetic selections that use proteinaceous transdominant inhibitors encoded by DNA libraries to cause mutant phenocopies may facilitate genetic analysis in traditionally nongenetic organisms. We...

Oligonucleotide-conjugated beads for transdominant genetic experiments

Feldhaus, Michael J., Lualhati, Michael, Cardon, Karen, Roth, Bruce, Kamb, Alexander

Transdominant genetics using expression libraries can identify proteins and peptides that affect cell division. In conjunction with these libraries, oligonucleotide-conjugated beads and flow...

Genetic selection for modulators of a retinoic-acid-responsive reporter in human cells.

Richards, Burt, Karpilow, Jon, Dunn, Christine, Peterson, Isaac, Maxfield, Andrew, Zharkikh, Ludmilla, ...

We used a genetic screening methodology, a human cell line bearing a retinoic-acid-responsive enhanced GFP reporter, and a flow sorter to recover dominant modulators of reporter expression. Four...

Transdominant genetic analysis of a growth control pathway

Caponigro, Giordano, Abedi, Majid R., Hurlburt, Anthony P., Maxfield, Andrew, Judd, Weston, Kamb, Alexander

Genetic selections that use proteinaceous transdominant inhibitors encoded by DNA libraries to cause mutant phenocopies may facilitate genetic analysis in traditionally nongenetic organisms. We...

Oligonucleotide-conjugated beads for transdominant genetic experiments

Feldhaus, Michael J., Lualhati, Michael, Cardon, Karen, Roth, Bruce, Kamb, Alexander

Transdominant genetics using expression libraries can identify proteins and peptides that affect cell division. In conjunction with these libraries, oligonucleotide-conjugated beads and flow...

Genetic selection for modulators of a retinoic-acid-responsive reporter in human cells.

Richards, Burt, Karpilow, Jon, Dunn, Christine, Peterson, Isaac, Maxfield, Andrew, Zharkikh, Ludmilla, ...

We used a genetic screening methodology, a human cell line bearing a retinoic-acid-responsive enhanced GFP reporter, and a flow sorter to recover dominant modulators of reporter expression. Four...

Genetic Evidence in Melanoma and Bladder Cancers that p16 and p53 Function in Separate Pathways of Tumor Suppression

Gruis, Nelleke A., Weaver-Feldhaus, Jane, Liu, Qingyun, Frye, Cheryl, Eeles, Rosalind, Orlow, Irene, ...

The 9p21 region of human chromosome 9 is a hot spot for chromosomal aberrations in both cultured cell lines and primary tumors. This region contains a gene, P16 (also called MTS1, CDKN2 and p16INK4),...