Polack, Fernando P., Lee, Sok H., Permar, Sallie R., Manyara, Elizabeth, Nousari, Hossein G., Jeng, Yaikah, ...
Measles remains a principal cause of worldwide mortality, in part because young infants cannot be immunized effectively. Development of new vaccines has been hindered by previous experience with a...
Early studies on DNA-based immunizations for measles virus (1997)
Yang, Hyekyung, Mustafa, Farah, Valsamakis, Alexandra, Santoro, Joseph C., Griffin, Diane E., Robinson, Harriet L.
DNA-mediated immunizations have been used to raise neutralizing antibodies for measles virus. Single inoculations of plasmids expressing measles hemagglutinin or fusion glycoproteins raised...
Thesis (Ph. D.)--University of Pennsylvania, 1992.
Valsamakis, Alexandra, Schneider, Henriette, Auwaerter, Paul G., Kaneshima, Hideto, Billeter, Martin A., Griffin, Diane E.
An understanding of the determinants of measles virus (MV) virulence has been hampered by the lack of an experimental model of infection. We have previously demonstrated that virulence phenotypes in...
Clinical Isolates of Measles Virus Use CD46 as a Cellular Receptor†
Manchester, Marianne, Eto, Danelle S., Valsamakis, Alexandra, Liton, Paloma B., Fernandez-Muñoz, Rafael, Rota, Paul A., ...
Laboratory strains of measles viruses (MV), such as Edmonston and Halle, use the complement regulatory protein CD46 as a cell surface receptor. The receptor usage of clinical isolates of MV, however,...
Altered Virulence of Vaccine Strains of Measles Virus after Prolonged Replication in Human Tissue
Valsamakis, Alexandra, Auwaerter, Paul G., Rima, Bert K., Kaneshima, Hideto, Griffin, Diane E.
To understand the molecular determinants of measles virus (MV) virulence, we have used the SCID-hu thymus/liver xenograft model (SCID-hu thy/liv) in which in vivo MV virulence phenotypes are...
Comparison of Automated and Manual Nucleic Acid Extraction Methods for Detection of Enterovirus RNA
Knepp, Julia H., Geahr, Melissa A., Forman, Michael S., Valsamakis, Alexandra
Automated nucleic acid extraction is an attractive alternative to labor-intensive manual methods. We compared two automated methods, the BioRobot M48 instrument (Qiagen, Inc.) and MagNA Pure (Roche...
Elbeik, Tarek, Surtihadi, Johan, Destree, Mark, Gorlin, Jed, Holodniy, Mark, Jortani, Saeed A., ...
In this multicenter evaluation, the VERSANT HCV RNA 3.0 Assay (bDNA) (Bayer Diagnostics, Tarrytown, N.Y.) was shown to have excellent reproducibility, linearity, and analytical sensitivity across...
Forman, Michael S., Valsamakis, Alexandra
Protocols were designed for quantification and detection of hepatitis C virus (HCV) RNA by the use of an analyte-specific reagent (ASR) (Roche COBAS TaqMan48 [CTM48] HCV) after manual and automated...
Pan, Chien-Hsiung, Valsamakis, Alexandra, Colella, Teresa, Nair, Nitya, Adams, Robert J., Polack, Fernando P., ...
Measles remains a major worldwide problem partly because of difficulties with vaccination of young infants. New vaccine strategies need to be safe and to provide sustained protective immunity. We...
Borek, Anita P., Clemens, Susan H., Gaskins, Valerie K., Aird, Deborah Z., Valsamakis, Alexandra
The performance characteristics of Xpect RSV (XP) and Binax Now RSV (BN) were compared to those of direct fluorescent-antibody staining and/or tissue culture for detection of respiratory syncytial...
Valsamakis, Alexandra, Schneider, Henriette, Auwaerter, Paul G., Kaneshima, Hideto, Billeter, Martin A., Griffin, Diane E.
An understanding of the determinants of measles virus (MV) virulence has been hampered by the lack of an experimental model of infection. We have previously demonstrated that virulence phenotypes in...
Clinical Isolates of Measles Virus Use CD46 as a Cellular Receptor†
Manchester, Marianne, Eto, Danelle S., Valsamakis, Alexandra, Liton, Paloma B., Fernandez-Muñoz, Rafael, Rota, Paul A., ...
Laboratory strains of measles viruses (MV), such as Edmonston and Halle, use the complement regulatory protein CD46 as a cell surface receptor. The receptor usage of clinical isolates of MV, however,...
Altered Virulence of Vaccine Strains of Measles Virus after Prolonged Replication in Human Tissue
Valsamakis, Alexandra, Auwaerter, Paul G., Rima, Bert K., Kaneshima, Hideto, Griffin, Diane E.
To understand the molecular determinants of measles virus (MV) virulence, we have used the SCID-hu thymus/liver xenograft model (SCID-hu thy/liv) in which in vivo MV virulence phenotypes are...
Comparison of Automated and Manual Nucleic Acid Extraction Methods for Detection of Enterovirus RNA
Knepp, Julia H., Geahr, Melissa A., Forman, Michael S., Valsamakis, Alexandra
Automated nucleic acid extraction is an attractive alternative to labor-intensive manual methods. We compared two automated methods, the BioRobot M48 instrument (Qiagen, Inc.) and MagNA Pure (Roche...
Elbeik, Tarek, Surtihadi, Johan, Destree, Mark, Gorlin, Jed, Holodniy, Mark, Jortani, Saeed A., ...
In this multicenter evaluation, the VERSANT HCV RNA 3.0 Assay (bDNA) (Bayer Diagnostics, Tarrytown, N.Y.) was shown to have excellent reproducibility, linearity, and analytical sensitivity across...
Forman, Michael S., Valsamakis, Alexandra
Protocols were designed for quantification and detection of hepatitis C virus (HCV) RNA by the use of an analyte-specific reagent (ASR) (Roche COBAS TaqMan48 [CTM48] HCV) after manual and automated...
Pan, Chien-Hsiung, Valsamakis, Alexandra, Colella, Teresa, Nair, Nitya, Adams, Robert J., Polack, Fernando P., ...
Measles remains a major worldwide problem partly because of difficulties with vaccination of young infants. New vaccine strategies need to be safe and to provide sustained protective immunity. We...
Borek, Anita P., Clemens, Susan H., Gaskins, Valerie K., Aird, Deborah Z., Valsamakis, Alexandra
The performance characteristics of Xpect RSV (XP) and Binax Now RSV (BN) were compared to those of direct fluorescent-antibody staining and/or tissue culture for detection of respiratory syncytial...
Forman, Michael Stuart, Valsamakis, Alexandra
Processing modifications were made to the COBAS AMPLICOR HCV version 2.0 assay to enhance sensitivity. Two methods of specimen concentration, centrifugation (“ultraspin”) and cationic detergent...
Molecular Testing in the Diagnosis and Management of Chronic Hepatitis B
Hepatitis B virus (HBV) is an enveloped virus with a small (3.2-kb) partially double-stranded DNA genome that causes acute and chronic infections. The impact of these infections on public health...
Forman, Michael S., Valsamakis, Alexandra
Performance characteristics of a hepatitis C virus (HCV) RNA quantification assay comprised automated specimen extraction [COBAS AmpliPrep (CAP) using total nucleic acid isolation reagents (TNAI)],...