Alfred M. Handler

The lepidopteran transposon vector, piggyBac, mediates germ-line transformation in the Mediterranean fruit fly

Handler, Alfred M., McCombs, Susan D., Fraser, Malcolm J., Saul, Stephen H.

The piggyBac (IFP2) short inverted terminal repeat transposable element from the cabbage looper Trichoplusia ni was tested for gene transfer vector function as part of a bipartite vector–helper...

Mobility of P elements in drosophilids and nondrosophilids

O'Brochta, David A., Handler, Alfred M.

The mobility properties of the Drosophila melanogaster P element in drosophilid and nondrosophilid species has been determined using a P-element mobility assay that is conducted transiently in insect...

Site-specific genomic targeting in Drosophila

Horn, Carsten, Handler, Alfred M.

Transcriptional regulation of transgenes depends upon genomic localization in higher eukaryotes. For the applied use of transgenic organisms as producers of pharmaceutically relevant proteins or as...

The lepidopteran transposon vector, piggyBac, mediates germ-line transformation in the Mediterranean fruit fly

Handler, Alfred M., McCombs, Susan D., Fraser, Malcolm J., Saul, Stephen H.

The piggyBac (IFP2) short inverted terminal repeat transposable element from the cabbage looper Trichoplusia ni was tested for gene transfer vector function as part of a bipartite vector–helper...

Mobility of P elements in drosophilids and nondrosophilids

O'Brochta, David A., Handler, Alfred M.

The mobility properties of the Drosophila melanogaster P element in drosophilid and nondrosophilid species has been determined using a P-element mobility assay that is conducted transiently in insect...

Site-specific genomic targeting in Drosophila

Horn, Carsten, Handler, Alfred M.

Transcriptional regulation of transgenes depends upon genomic localization in higher eukaryotes. For the applied use of transgenic organisms as producers of pharmaceutically relevant proteins or as...

piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells

Wu, Sareina Chiung-Yuan, Meir, Yaa-Jyuhn James, Coates, Craig J., Handler, Alfred M., Pelczar, Pawel, Moisyadi, Stefan, ...

A nonviral vector for highly efficient site-specific integration would be desirable for many applications in transgenesis, including gene therapy. In this study we directly compared the genomic...