Alfred Pühler

EDGAR: A software framework for the comparative analysis of prokaryotic genomes (2009)

Blom, Jochen, Albaum, Stefan P, Doppmeier, Daniel, Pühler, Alfred, Vorhölter, Frank-Jörg, Zakrzewski, Martha, ...

Abstract Background The introduction of next generation sequencing approaches has caused a rapid increase in the number of completely sequenced genomes. As one result of this development, it is now...

The time course of the transcriptomic response of Sinorhizobium meliloti1021 following a shift to acidic pH (2009)

Hellweg, Christoph, Pühler, Alfred, Weidner, Stefan

Abstract Background The symbiotic soil bacterium Sinorhizobium meliloti often has to face low pH in its natural habitats. To identify genes responding to pH stress a global transcriptional analysis...

EMMA 2 – A MAGE-compliant system for the collaborative analysis and integration of microarray data (2009)

Dondrup, Michael, Albaum, Stefan P, Griebel, Thasso, Henckel, Kolja, Jünemann, Sebastian, Kahlke, Tim, ...

Abstract Background Understanding transcriptional regulation by genome-wide microarray studies can contribute to unravel complex relationships between genes. Attempts to standardize the annotation of...

The dual transcriptional regulator CysR in Corynebacterium glutamicumATCC 13032 controls a subset of genes of the McbR regulon in response to the availability of sulphide acceptor molecules (2008)

Rückert, Christian, Milse, Johanna, Albersmeier, Andreas, Koch, Daniel J, Pühler, Alfred, Kalinowski, Jörn

Abstract Background Regulation of sulphur metabolism in Corynebacterium glutamicum ATCC 13032 has been studied intensively in the last few years, due to its industrial as well as scientific...

The dual transcriptional regulator CysR in Corynebacterium glutamicum ATCC 13032 controls a subset of genes of the McbR regulon in response to the availability of sulphide acceptor molecules (2008)

Rückert, Christian, Milse, Johanna, Albersmeier, Andreas, Koch, Daniel J., Pühler, Alfred, Kalinowski, Jörn

Background: Regulation of sulphur metabolism in Corynebacterium glutamicum ATCC 13032 has been studied intensively in the last few years, due to its industrial as well as scientific importance....

The missing link: Bordetella petriiis endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae (2008)

Gross, Roy, Guzman, Carlos A, Sebaihia, Mohammed, Martins Dos Santos, Vítor AP, Pieper, Dietmar H, Koebnik, Ralf, ...

Abstract Background Bordetella petrii is the only environmental species hitherto found among the otherwise host-restricted and pathogenic members of the genus Bordetella . Phylogenetically, it...

Der Beitrag von Pflanzen mit neuartigen Eigenschaften zur Inwertsetzung ländlicher Räume (2008)

Serr, Anke, Pühler, Alfred, Broer, Inge

In den nächsten 20 Jahren müssen Landwirte mit Beihilfekürzungen, klimatischen Veränderungen, sich wandelnden Absatzmärkten durch verändertes Verbraucherverhalten und steigendem Bedarf an...

CoryneCenter – An online resource for the integrated analysis of corynebacterial genome and transcriptome data (2007)

Neuweger, Heiko, Baumbach, Jan, Albaum, Stefan, Bekel, Thomas, Dondrup, Michael, Hüser, Andrea T, ...

Abstract Background The introduction of high-throughput genome sequencing and post-genome analysis technologies, e.g. DNA microarray approaches, has created the potential to unravel and scrutinize...

The DeoR-type transcriptional regulator SugR acts as a repressor for genes encoding the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in Corynebacterium glutamicum (2007)

Gaigalat, Lars, Schlüter, Jan-Philip, Hartmann, Michelle, Mormann, Sascha, Tauch, Andreas, Pühler, Alfred, ...

Abstract Background The major uptake system responsible for the transport of fructose, glucose, and sucrose in Corynebacterium glutamicum ATCC 13032 is the phosphoenolpyruvate:sugar...

GISMO--gene identification using a support vector machine for ORF classification (2007)

Krause, Lutz, McHardy, Alice C., Nattkemper, Tim W., Pühler, Alfred, Stoye, Jens, Meyer, Folker

We present the novel prokaryotic gene finder GISMO, which combines searches for protein family domains with composition-based classification based on a support vector machine. GISMO is highly...

Random mutagenesis in Corynebacterium glutamicumATCC 13032 using an IS6100-based transposon vector identified the last unknown gene in the histidine biosynthesis pathway (2006)

Mormann, Sascha, Lömker, Alexander, Rückert, Christian, Gaigalat, Lars, Tauch, Andreas, Pühler, Alfred, ...

Abstract Background Corynebacterium glutamicum , a Gram-positive bacterium of the class Actinobacteria, is an industrially relevant producer of amino acids. Several methods for the targeted genetic...

The DtxR protein acting as dual transcriptional regulator directs a global regulatory network involved in iron metabolism of Corynebacterium glutamicum (2006)

Brune, Iris, Werner, Hendrikje, Hüser, Andrea T, Kalinowski, Jörn, Pühler, Alfred, Tauch, Andreas

Abstract Background The knowledge about complete bacterial genome sequences opens the way to reconstruct the qualitative topology and global connectivity of transcriptional regulatory networks. Since...

GISMO--gene identification using a support vector machine for ORF classification (2006)

Krause, Lutz, McHardy, Alice C., Nattkemper, Tim W., Pühler, Alfred, Stoye, Jens, Meyer, Folker

We present the novel prokaryotic gene finder GISMO, which combines searches for protein family domains with composition-based classification based on a support vector machine. GISMO is highly...

Finding novel genes in bacterial communities isolated from the environment (2006)

Krause, Lutz, Diaz, Naryttza N., Bartels, Daniela, Edwards, Robert A., Pühler, Alfred, Rohwer, Forest, ...

Motivation: Novel sequencing techniques can give access to organisms that are difficult to cultivate using conventional methods. When applied to environmental samples, the data generated has some...

GISMO--gene identification using a support vector machine for ORF classification (2006)

Krause, Lutz, McHardy, Alice C., Nattkemper, Tim W., Pühler, Alfred, Stoye, Jens, Meyer, Folker

We present the novel prokaryotic gene finder GISMO, which combines searches for protein family domains with composition-based classification based on a support vector machine. GISMO is highly...

Functional genomics and expression analysis of the Corynebacterium glutamicum fpr2-cysIXHDNYZ gene cluster involved in assimilatory sulphate reduction (2005)

Rückert, Christian, Koch, Daniel J, Rey, Daniel A, Albersmeier, Andreas, Mormann, Sascha, Pühler, Alfred, ...

Abstract Background Corynebacterium glutamicum is a high-GC Gram-positive soil bacterium of great biotechnological importance for the production of amino acids. To facilitate the rational design of...

The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium efficiens, Corynebacterium diphtheriaeand Corynebacterium jeikeiumdeduced from the complete genome sequences (2005)

Brune, Iris, Brinkrolf, Karina, Kalinowski, Jörn, Pühler, Alfred, Tauch, Andreas

Abstract Background The genus Corynebacterium includes Gram-positive microorganisms of great biotechnologically importance, such as Corynebacterium glutamicum and Corynebacterium efficiens , as well...

BACCardI--a tool for the validation of genomic assemblies, assisting genome finishing and intergenome comparison (2005)

Bartels, Daniela, Kespohl, Sebastian, Albaum, Stefan, Drüke, Tanja, Goesmann, Alexander, Herold, Julia, ...

Summary: We provide the graphical tool BACCardI for the construction of virtual clone maps from standard assembler output files or BLAST based sequence comparisons. This new tool has been applied to...

BMC Genomics BioMed Central (2005)

Iris Brune, Karina Brinkrolf, Jörn Kalinowski, Alfred Pühler, Andreas Tauch, Andreas Tauch

Research article The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium efficiens, Corynebacterium diphtheriae and...

BIOINFORMATICS ORIGINAL PAPER (2004)

Genome Analysis, Daniela Bartels, Sebastian Kespohl, Stefan Albaum, Tanja Drüke, Er Goesmann, ...

BACCardI—a tool for the validation of genomic assemblies, assisting genome finishing and intergenome comparison

Development of joint application strategies for two microbial gene finders (2004)

McHardy, Alice C., Goesmann, Alexander, Pühler, Alfred, Meyer, Folker

Motivation: As a starting point in annotation of bacterial genomes, gene finding programs are used for the prediction of functional elements in the DNA sequence. Due to the faster pace and increasing...

Development of joint application strategies for two microbial gene finders (2004)

McHardy, Alice C., Goesmann, Alexander, Pühler, Alfred, Meyer, Folker

Motivation: As a starting point in annotation of bacterial genomes, gene finding programs are used for the prediction of functional elements in the DNA sequence. Due to the faster pace and increasing...

BACCardI - a tool for the validation of genomic assemblies, assisting genome finishing and intergenome comparison (2004)

Bartels, Daniela, Kespohl, Sebastian, Albaum, Stefan, Drüke, Tanja, Goesmann, Alexander, Herold, Julia, ...

Motivation: Genomic assemblies generated from sequence information need to be validated by independent methods such as physical maps. The time-consuming task of building physical maps can be...

Development of joint application strategies for two microbial gene finders (2004)

McHardy, Alice C., Goesmann, Alexander, Pühler, Alfred, Meyer, Folker

Motivation: As a starting point in annotation of bacterial genomes, gene finding programs are used for the prediction of functional elements in the DNA sequence. Due to the faster pace and increasing...

BACCardI - a tool for the validation of genomic assemblies, assisting genome finishing and intergenome comparison (2004)

Bartels, Daniela, Kespohl, Sebastian, Albaum, Stefan, Drüke, Tanja, Goesmann, Alexander, Herold, Julia, ...

Motivation: Genomic assemblies generated from sequence information need to be validated by independent methods such as physical maps. The time-consuming task of building physical maps can be...

GenDB--an open source genome annotation system for prokaryote genomes (2003)

Meyer, Folker, Goesmann, Alexander, McHardy, Alice C., Bartels, Daniela, Bekel, Thomas, Clausen, Jörn, ...

The flood of sequence data resulting from the large number of current genome projects has increased the need for a flexible, open source genome annotation system, which so far has not existed. To...

The complete nucleotide sequence and environmental distribution of the cryptic, conjugative, broad-host-range plasmid pIPO2 isolated from bacteria of the wheat rhizosphere (2002)

Tauch,Andreas, Schneiker,Susanne, Selbitschka,Werner, Pühler,Alfred, Van Overbeek,Leo S., Smalla,Kornelia, ...

Plasmid pIPO2 is a cryptic, conjugative, broad-host-range plasmid isolated from the wheat rhizosphere. It efficiently self-transfers between , ß and Proteobacteria and has a...

The complete nucleotide sequence and environmental distribution of the cryptic, conjugative, broad-host-range plasmid pIPO2 isolated from bacteria of the wheat rhizosphere (2002)

Tauch, Andreas, Schneiker, Susanne, Selbitschka, Werner, Pühler, Alfred, Van Overbeek, Leo S., Smalla, Kornelia, ...

Plasmid pIPO2 is a cryptic, conjugative, broad-host-range plasmid isolated from the wheat rhizosphere. It efficiently self-transfers between , ß and Proteobacteria and has a...

The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa (2001)

Schneiker, Susanne, Keller, Mathias, Dröge, Marcus, Lanka, Erich, Pühler, Alfred, Selbitschka, Werner

Employing the biparental exogenous plasmid isolation method, conjugative plasmids conferring mercury resistance were isolated from the microbial community of the rhizosphere of field grown alfalfa...

Genetic Characterization of a Sinorhizobium meliloti Chromosomal Region Involved in (2000)

Lipopolysaccharide Biosynthesis, Antonio Lagares, Daniela F. Hozbor, Karsten Niehaus, Jens Lorenzen, ...

The genetic characterization of a 5.5-kb chromosomal region of Sinorhizobium meliloti 2011 that contains lpsB, a gene required for the normal development of symbiosis with Medicago spp., is...

Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame (1987)

Grönger, Petra, Manian, Sundaram S., Reiländer, Helmut, O'Connell, Michael, Priefer, Ursula B., Pühler, Alfred

By hybridization and heteroduplex studies the fixABC and nifA genes of the Rhizobium leguminosarum symbiotic plasmid pRL6JI have been identified. DNA sequencing of the region containing nifA showed...

Expression of plant tumor-specific proteins in minicells of Escherichia coli: a fusion protein of lysopine dehydrogenase with chloramphenicol acetyltransferase (1981)

Schröder, Joachim, Hillebrand, Annette, Klipp, Werner, Pühler, Alfred

Fragment EcoRI 7 from Ti-plasmid pTi Ach5, a part of the T-DNA in octo-pine tumors, was cloned in both orientations into pACYC184 and expressed in E.coli minicells. The cells synthesized four...

Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021

Capela, Delphine, Barloy-Hubler, Frédérique, Gouzy, Jérôme, Bothe, Gordana, Ampe, Frédéric, Batut, Jacques, ...

Sinorhizobium meliloti is an α-proteobacterium that forms agronomically important N2-fixing root nodules in legumes. We report here the complete sequence of the largest constituent of its genome, a...

The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti

Finan, Turlough M., Weidner, Stefan, Wong, Kim, Buhrmester, Jens, Chain, Patrick, Vorhölter, Frank J., ...

Analysis of the 1,683,333-nt sequence of the pSymB megaplasmid from the symbiotic N2-fixing bacterium Sinorhizobium meliloti revealed that the replicon has a high gene density with a total of 1,570...

Isolation and Characterization of Alfalfa-Nodulating Rhizobia Present in Acidic Soils of Central Argentina and Uruguay

Del Papa, María F., Balagué, Laura J., Sowinski, Susana Castro, Wegener, Caren, Segundo, Eduardo, Abarca, Francisco Martínez, ...

We describe the isolation and characterization of alfalfa-nodulating rhizobia from acid soils of different locations in Central Argentina and Uruguay. A collection of 465 isolates was assembled, and...

Expression of the Corynebacterium glutamicum panD Gene Encoding l-Aspartate-α-Decarboxylase Leads to Pantothenate Overproduction in Escherichia coli

Dusch, Nicole, Pühler, Alfred, Kalinowski, Jörn

The Corynebacterium glutamicum panD gene was identified by functional complementation of an Escherichia coli panD mutant strain. Sequence analysis revealed that the coding region of panD comprises...

Genetic Characterization of a Sinorhizobium meliloti Chromosomal Region Involved in Lipopolysaccharide Biosynthesis

Lagares, Antonio, Hozbor, Daniela F., Niehaus, Karsten, Lorenzen, Jens, Arnold, Walter, ...

The genetic characterization of a 5.5-kb chromosomal region of Sinorhizobium meliloti 2011 that contains lpsB, a gene required for the normal development of symbiosis with Medicago spp., is...

The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa

Schneiker, Susanne, Keller, Mathias, Dröge, Marcus, Lanka, Erich, Pühler, Alfred, Selbitschka, Werner

Employing the biparental exogenous plasmid isolation method, conjugative plasmids conferring mercury resistance were isolated from the microbial community of the rhizosphere of field grown alfalfa...

Prevalence of the Rhizobium etli-Like Allele in Genes Coding for 16S rRNA among the Indigenous Rhizobial Populations Found Associated with Wild Beans from the Southern Andes in Argentina

Aguilar, O. Mario, López, María Verónica, Riccillo, Pablo M., González, Ramón A., Pagano, Marcela, Grasso, Daniel H., ...

A collection of rhizobial isolates from nodules of wild beans, Phaseolus vulgaris var. aborigineus, found growing in virgin lands in 17 geographically separate sites in northwest Argentina was...

Specific Amino Acid Substitutions in the Proline-Rich Motif of the Rhizobium meliloti ExoP Protein Result in Enhanced Production of Low-Molecular-Weight Succinoglycan at the Expense of High-Molecular-Weight Succinoglycan

Becker, Anke, Pühler, Alfred

The production of the acidic exopolysaccharide succinoglycan (EPS I) by Rhizobium meliloti exoP* mutants expressing an ExoP protein lacking its C-terminal cytoplasmic domain and by mutants...

Xanthomonas campestris pv. campestris gum Mutants: Effects on Xanthan Biosynthesis and Plant Virulence

Katzen, Federico, Ferreiro, Diego U., Oddo, Cristian G., Ielmini, M. Verónica, Becker, Anke, Pühler, Alfred, ...

Xanthan is an industrially important exopolysaccharide produced by the phytopathogenic, gram-negative bacterium Xanthomonas campestris pv. campestris. It is composed of polymerized pentasaccharide...

Identification of Two prpDBC Gene Clusters in Corynebacterium glutamicum and Their Involvement in Propionate Degradation via the 2-Methylcitrate Cycle

Claes, Wilfried A., Pühler, Alfred, Kalinowski, Jörn

Genome sequencing revealed that the Corynebacterium glutamicum genome contained, besides gltA, two additional citrate synthase homologous genes (prpC) located in two different prpDBC gene clusters,...

GenDB—an open source genome annotation system for prokaryote genomes

Meyer, Folker, Goesmann, Alexander, McHardy, Alice C., Bartels, Daniela, Bekel, Thomas, Clausen, Jörn, ...

The flood of sequence data resulting from the large number of current genome projects has increased the need for a flexible, open source genome annotation system, which so far has not existed. To...

Conjugal Transfer of Megaplasmid 2 between Rhizobium meliloti Strains in Alfalfa Nodules

Pretorius-Güth, Inge-M., Pühler, Alfred, Simon, Reinhard

A DNA fragment containing the RP4 mob function, as well as the gentamicin and spectinomycin resistance genes, was inserted by gene replacement onto the megaplasmid 2 (pM2) of Rhizobium meliloti 0540...

The Sinorhizobium meliloti fur Gene Regulates, with Dependence on Mn(II), Transcription of the sitABCD Operon, Encoding a Metal-Type Transporter

Chao, Tzu-Chiao, Becker, Anke, Buhrmester, Jens, Pühler, Alfred, Weidner, Stefan

Sinorhizobium meliloti is an alpha-proteobacterium able to induce nitrogen-fixing nodules on roots of specific legumes. In order to propagate in the soil and for successful symbiotic interaction the...

Expression Profiling in Medicago truncatula Identifies More Than 750 Genes Differentially Expressed during Nodulation, Including Many Potential Regulators of the Symbiotic Program1[w]

El Yahyaoui, Fikri, Küster, Helge, Amor, Besma Ben, Hohnjec, Natalija, Pühler, Alfred, Becker, Anke, ...

In this study, we describe a large-scale expression-profiling approach to identify genes differentially regulated during the symbiotic interaction between the model legume Medicago truncatula and the...

Mapping and expression of a regulatory nitrogen fixation gene (fixD) of Rhizobium meliloti

Weber, Gerhard, Reiländer, Helmut, Pühler, Alfred

A 3.5-kb HindIII fragment from the main nif/fix (nitrogen fixation) gene cluster of Rhizobium meliloti was characterized by studying its expression in Escherichia coli minicells. A coding region for...

Adaptation of Corynebacterium glutamicum to Ammonium Limitation: a Global Analysis Using Transcriptome and Proteome Techniques†

Silberbach, Maike, Schäfer, Mathias, Hüser, Andrea T., Kalinowski, Jörn, Pühler, Alfred, Krämer, Reinhard, ...

Theresponse of Corynebacterium glutamicum to ammonium limitation was studied by transcriptional and proteome profiling of cells grown in a chemostat. Our results show that ammonium-limited growth of...

Rational Design of a Corynebacterium glutamicum Pantothenate Production Strain and Its Characterization by Metabolic Flux Analysis and Genome-Wide Transcriptional Profiling

Hüser, Andrea T., Chassagnole, Christophe, Lindley, Nic D., Merkamm, Muriel, Guyonvarch, Armel, Elišáková, Veronika, ...

A “second-generation” production strain was derived from a Corynebacterium glutamicum pantothenate producer by rational design to assess its potential to synthesize and accumulate the vitamin...

Role of the Regulatory Gene rirA in the Transcriptional Response of Sinorhizobium meliloti to Iron Limitation†

Chao, Tzu-Chiao, Buhrmester, Jens, Hansmeier, Nicole, Pühler, Alfred, Weidner, Stefan

A regulatory network of Sinorhizobium meliloti genes involved in adaptation to iron-limiting conditions and the involvement of the rhizobial iron regulator gene (rirA) were analyzed by mutation and...

Insights into Genome Plasticity and Pathogenicity of the Plant Pathogenic Bacterium Xanthomonas campestris pv. vesicatoria Revealed by the Complete Genome Sequence

Thieme, Frank, Koebnik, Ralf, Bekel, Thomas, Berger, Carolin, Boch, Jens, Büttner, Daniela, ...

The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically important...

Overlaps in the Transcriptional Profiles of Medicago truncatula Roots Inoculated with Two Different Glomus Fungi Provide Insights into the Genetic Program Activated during Arbuscular Mycorrhiza1[w]

Hohnjec, Natalija, Vieweg, Martin F., Pühler, Alfred, Becker, Anke, Küster, Helge

Arbuscular mycorrhiza (AM) is a widespread symbiotic association between plants and fungal microsymbionts that supports plant development under nutrient-limiting and various stress conditions. In...

Complete Genome Sequence and Analysis of the Multiresistant Nosocomial Pathogen Corynebacterium jeikeium K411, a Lipid-Requiring Bacterium of the Human Skin Flora

Tauch, Andreas, Kaiser, Olaf, Hain, Torsten, Goesmann, Alexander, Weisshaar, Bernd, Albersmeier, Andreas, ...

Corynebacterium jeikeium is a “lipophilic” and multidrug-resistant bacterial species of the human skin flora that has been recognized with increasing frequency as a serious nosocomial pathogen....

The clc Element of Pseudomonas sp. Strain B13, a Genomic Island with Various Catabolic Properties

Gaillard, Muriel, Vallaeys, Tatiana, Vorhölter, Frank Jörg, Minoia, Marco, Werlen, Christoph, Sentchilo, Vladimir, ...

Pseudomonas sp. strain B13 is a bacterium known to degrade chloroaromatic compounds. The properties to use 3- and 4-chlorocatechol are determined by a self-transferable DNA element, the clc element,...

Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021

Capela, Delphine, Barloy-Hubler, Frédérique, Gouzy, Jérôme, Bothe, Gordana, Ampe, Frédéric, Batut, Jacques, ...

Sinorhizobium meliloti is an α-proteobacterium that forms agronomically important N2-fixing root nodules in legumes. We report here the complete sequence of the largest constituent of its genome, a...

The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti

Finan, Turlough M., Weidner, Stefan, Wong, Kim, Buhrmester, Jens, Chain, Patrick, Vorhölter, Frank J., ...

Analysis of the 1,683,333-nt sequence of the pSymB megaplasmid from the symbiotic N2-fixing bacterium Sinorhizobium meliloti revealed that the replicon has a high gene density with a total of 1,570...

Isolation and Characterization of Alfalfa-Nodulating Rhizobia Present in Acidic Soils of Central Argentina and Uruguay

Del Papa, María F., Balagué, Laura J., Sowinski, Susana Castro, Wegener, Caren, Segundo, Eduardo, Abarca, Francisco Martínez, ...

We describe the isolation and characterization of alfalfa-nodulating rhizobia from acid soils of different locations in Central Argentina and Uruguay. A collection of 465 isolates was assembled, and...

Expression of the Corynebacterium glutamicum panD Gene Encoding l-Aspartate-α-Decarboxylase Leads to Pantothenate Overproduction in Escherichia coli

Dusch, Nicole, Pühler, Alfred, Kalinowski, Jörn

The Corynebacterium glutamicum panD gene was identified by functional complementation of an Escherichia coli panD mutant strain. Sequence analysis revealed that the coding region of panD comprises...

Genetic Characterization of a Sinorhizobium meliloti Chromosomal Region Involved in Lipopolysaccharide Biosynthesis

Lagares, Antonio, Hozbor, Daniela F., Niehaus, Karsten, Lorenzen, Jens, Arnold, Walter, ...

The genetic characterization of a 5.5-kb chromosomal region of Sinorhizobium meliloti 2011 that contains lpsB, a gene required for the normal development of symbiosis with Medicago spp., is...

The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa

Schneiker, Susanne, Keller, Mathias, Dröge, Marcus, Lanka, Erich, Pühler, Alfred, Selbitschka, Werner

Employing the biparental exogenous plasmid isolation method, conjugative plasmids conferring mercury resistance were isolated from the microbial community of the rhizosphere of field grown alfalfa...

Prevalence of the Rhizobium etli-Like Allele in Genes Coding for 16S rRNA among the Indigenous Rhizobial Populations Found Associated with Wild Beans from the Southern Andes in Argentina

Aguilar, O. Mario, López, María Verónica, Riccillo, Pablo M., González, Ramón A., Pagano, Marcela, Grasso, Daniel H., ...

A collection of rhizobial isolates from nodules of wild beans, Phaseolus vulgaris var. aborigineus, found growing in virgin lands in 17 geographically separate sites in northwest Argentina was...

Specific Amino Acid Substitutions in the Proline-Rich Motif of the Rhizobium meliloti ExoP Protein Result in Enhanced Production of Low-Molecular-Weight Succinoglycan at the Expense of High-Molecular-Weight Succinoglycan

Becker, Anke, Pühler, Alfred

The production of the acidic exopolysaccharide succinoglycan (EPS I) by Rhizobium meliloti exoP* mutants expressing an ExoP protein lacking its C-terminal cytoplasmic domain and by mutants...

Xanthomonas campestris pv. campestris gum Mutants: Effects on Xanthan Biosynthesis and Plant Virulence

Katzen, Federico, Ferreiro, Diego U., Oddo, Cristian G., Ielmini, M. Verónica, Becker, Anke, Pühler, Alfred, ...

Xanthan is an industrially important exopolysaccharide produced by the phytopathogenic, gram-negative bacterium Xanthomonas campestris pv. campestris. It is composed of polymerized pentasaccharide...

Identification of Two prpDBC Gene Clusters in Corynebacterium glutamicum and Their Involvement in Propionate Degradation via the 2-Methylcitrate Cycle

Claes, Wilfried A., Pühler, Alfred, Kalinowski, Jörn

Genome sequencing revealed that the Corynebacterium glutamicum genome contained, besides gltA, two additional citrate synthase homologous genes (prpC) located in two different prpDBC gene clusters,...

GenDB—an open source genome annotation system for prokaryote genomes

Meyer, Folker, Goesmann, Alexander, McHardy, Alice C., Bartels, Daniela, Bekel, Thomas, Clausen, Jörn, ...

The flood of sequence data resulting from the large number of current genome projects has increased the need for a flexible, open source genome annotation system, which so far has not existed. To...

Conjugal Transfer of Megaplasmid 2 between Rhizobium meliloti Strains in Alfalfa Nodules

Pretorius-Güth, Inge-M., Pühler, Alfred, Simon, Reinhard

A DNA fragment containing the RP4 mob function, as well as the gentamicin and spectinomycin resistance genes, was inserted by gene replacement onto the megaplasmid 2 (pM2) of Rhizobium meliloti 0540...

The Sinorhizobium meliloti fur Gene Regulates, with Dependence on Mn(II), Transcription of the sitABCD Operon, Encoding a Metal-Type Transporter

Chao, Tzu-Chiao, Becker, Anke, Buhrmester, Jens, Pühler, Alfred, Weidner, Stefan

Sinorhizobium meliloti is an alpha-proteobacterium able to induce nitrogen-fixing nodules on roots of specific legumes. In order to propagate in the soil and for successful symbiotic interaction the...

Expression Profiling in Medicago truncatula Identifies More Than 750 Genes Differentially Expressed during Nodulation, Including Many Potential Regulators of the Symbiotic Program1[w]

El Yahyaoui, Fikri, Küster, Helge, Amor, Besma Ben, Hohnjec, Natalija, Pühler, Alfred, Becker, Anke, ...

In this study, we describe a large-scale expression-profiling approach to identify genes differentially regulated during the symbiotic interaction between the model legume Medicago truncatula and the...

Mapping and expression of a regulatory nitrogen fixation gene (fixD) of Rhizobium meliloti

Weber, Gerhard, Reiländer, Helmut, Pühler, Alfred

A 3.5-kb HindIII fragment from the main nif/fix (nitrogen fixation) gene cluster of Rhizobium meliloti was characterized by studying its expression in Escherichia coli minicells. A coding region for...

Adaptation of Corynebacterium glutamicum to Ammonium Limitation: a Global Analysis Using Transcriptome and Proteome Techniques†

Silberbach, Maike, Schäfer, Mathias, Hüser, Andrea T., Kalinowski, Jörn, Pühler, Alfred, Krämer, Reinhard, ...

Theresponse of Corynebacterium glutamicum to ammonium limitation was studied by transcriptional and proteome profiling of cells grown in a chemostat. Our results show that ammonium-limited growth of...

Overlaps in the Transcriptional Profiles of Medicago truncatula Roots Inoculated with Two Different Glomus Fungi Provide Insights into the Genetic Program Activated during Arbuscular Mycorrhiza1[w]

Hohnjec, Natalija, Vieweg, Martin F., Pühler, Alfred, Becker, Anke, Küster, Helge

Arbuscular mycorrhiza (AM) is a widespread symbiotic association between plants and fungal microsymbionts that supports plant development under nutrient-limiting and various stress conditions. In...

Complete Genome Sequence and Analysis of the Multiresistant Nosocomial Pathogen Corynebacterium jeikeium K411, a Lipid-Requiring Bacterium of the Human Skin Flora

Tauch, Andreas, Kaiser, Olaf, Hain, Torsten, Goesmann, Alexander, Weisshaar, Bernd, Albersmeier, Andreas, ...

Corynebacterium jeikeium is a “lipophilic” and multidrug-resistant bacterial species of the human skin flora that has been recognized with increasing frequency as a serious nosocomial pathogen....

Rational Design of a Corynebacterium glutamicum Pantothenate Production Strain and Its Characterization by Metabolic Flux Analysis and Genome-Wide Transcriptional Profiling

Hüser, Andrea T., Chassagnole, Christophe, Lindley, Nic D., Merkamm, Muriel, Guyonvarch, Armel, Elišáková, Veronika, ...

A “second-generation” production strain was derived from a Corynebacterium glutamicum pantothenate producer by rational design to assess its potential to synthesize and accumulate the vitamin...

Role of the Regulatory Gene rirA in the Transcriptional Response of Sinorhizobium meliloti to Iron Limitation†

Chao, Tzu-Chiao, Buhrmester, Jens, Hansmeier, Nicole, Pühler, Alfred, Weidner, Stefan

A regulatory network of Sinorhizobium meliloti genes involved in adaptation to iron-limiting conditions and the involvement of the rhizobial iron regulator gene (rirA) were analyzed by mutation and...

Insights into Genome Plasticity and Pathogenicity of the Plant Pathogenic Bacterium Xanthomonas campestris pv. vesicatoria Revealed by the Complete Genome Sequence

Thieme, Frank, Koebnik, Ralf, Bekel, Thomas, Berger, Carolin, Boch, Jens, Büttner, Daniela, ...

The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically important...

The clc Element of Pseudomonas sp. Strain B13, a Genomic Island with Various Catabolic Properties

Gaillard, Muriel, Vallaeys, Tatiana, Vorhölter, Frank Jörg, Minoia, Marco, Werlen, Christoph, Sentchilo, Vladimir, ...

Pseudomonas sp. strain B13 is a bacterium known to degrade chloroaromatic compounds. The properties to use 3- and 4-chlorocatechol are determined by a self-transferable DNA element, the clc element,...

Mobilizable IncQ-Related Plasmid Carrying a New Quinolone Resistance Gene, qnrS2, Isolated from the Bacterial Community of a Wastewater Treatment Plant

Bönemann, Gabriele, Stiens, Michael, Pühler, Alfred, Schlüter, Andreas

Plasmid-encoded quinolone resistance was previously reported for different bacteria isolated from patients not only in the United States and Asia but also in Europe. Here we describe the isolation,...

Whole-Genome Sequence of Listeria welshimeri Reveals Common Steps in Genome Reduction with Listeria innocua as Compared to Listeria monocytogenes▿ †

Hain, Torsten, Steinweg, Christiane, Kuenne, Carsten Tobias, Billion, André, Ghai, Rohit, Chatterjee, Som Subhra, ...

We present the complete genome sequence of Listeria welshimeri, a nonpathogenic member of the genus Listeria. Listeria welshimeri harbors a circular chromosome of 2,814,130 bp with 2,780 open reading...

GISMO—gene identification using a support vector machine for ORF classification

Krause, Lutz, McHardy, Alice C., Nattkemper, Tim W., Pühler, Alfred, Stoye, Jens, Meyer, Folker

We present the novel prokaryotic gene finder GISMO, which combines searches for protein family domains with composition-based classification based on a support vector machine. GISMO is highly...

Novel Macrolide Resistance Module Carried by the IncP-1β Resistance Plasmid pRSB111, Isolated from a Wastewater Treatment Plant▿

Szczepanowski, Rafael, Krahn, Irene, Bohn, Nadine, Pühler, Alfred, Schlüter, Andreas

The macrolide resistance plasmid pRSB111 was isolated from bacteria residing in the final effluents of a wastewater treatment plant. The 47-kb plasmid confers resistance to azithromycin,...

The IclR-Type Transcriptional Repressor LtbR Regulates the Expression of Leucine and Tryptophan Biosynthesis Genes in the Amino Acid Producer Corynebacterium glutamicum▿

Brune, Iris, Jochmann, Nina, Brinkrolf, Karina, Hüser, Andrea T., Gerstmeir, Robert, Eikmanns, Bernhard J., ...

The transcriptional regulator Cg1486 of Corynebacterium glutamicum ATCC 13032 is a member of the IclR protein family and belongs to the conserved set of regulatory proteins in corynebacteria. A...

The Extracytoplasmic Function-Type Sigma Factor SigM of Corynebacterium glutamicum ATCC 13032 Is Involved in Transcription of Disulfide Stress-Related Genes▿

Nakunst, Diana, Larisch, Christof, Hüser, Andrea T., Tauch, Andreas, Pühler, Alfred, Kalinowski, Jörn

The gene for the extracytoplasmic function (ECF) sigma factor SigM was deleted from the chromosome of the gram-positive soil bacterium Corynebacterium glutamicum to elucidate the role of the SigM...

IncP-1β Plasmid pGNB1 Isolated from a Bacterial Community from a Wastewater Treatment Plant Mediates Decolorization of Triphenylmethane Dyes▿

Schlüter, Andreas, Krahn, Irene, Kollin, Florian, Bönemann, Gabriele, Stiens, Michael, Szczepanowski, Rafael, ...

Plasmid pGNB1 was isolated from bacteria residing in the activated sludge compartment of a wastewater treatment plant by using a transformation-based approach. This 60-kb plasmid confers resistance...

The Genome Sequence of the Tomato-Pathogenic Actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 Reveals a Large Island Involved in Pathogenicity▿ †

Gartemann, Karl-Heinz, Abt, Birte, Bekel, Thomas, Burger, Annette, Engemann, Jutta, Flügel, Monika, ...

Clavibacter michiganensis subsp. michiganensis is a plant-pathogenic actinomycete that causes bacterial wilt and canker of tomato. The nucleotide sequence of the genome of strain NCPPB382 was...

Bacteriophage 2851 Is a Prototype Phage for Dissemination of the Shiga Toxin Variant Gene 2c in Escherichia coli O157:H7▿ †

Strauch, Eckhard, Hammerl, Jens Andre, Konietzny, Antje, Schneiker-Bekel, Susanne, Arnold, Walter, Goesmann, Alexander, ...

The production of Shiga toxin (Stx) (verocytotoxin) is a major virulence factor of Escherichia coli O157:H7 strains (Shiga toxin-producing E. coli [STEC] O157). Two types of Shiga toxins, designated...

Genome sequence of Desulfobacterium autotrophicum HRM2, a marine sulfate reducer oxidizing organic carbon completely to carbon dioxide

Strittmatter, Axel W, Liesegang, Heiko, Rabus, Ralf, Decker, Iwona, Amann, Judith, Andres, Sönke, ...

Sulfate-reducing bacteria (SRB) belonging to the metabolically versatile Desulfobacteriaceae are abundant in marine sediments and contribute to the global carbon cycle by complete oxidation of...