Scientific Commons: Alfred Wittinghofer

Ras of complex proteins (Roc) belongs to the superfamily of Ras-related small G-proteins that always occurs in tandem with the C-terminal of Roc (COR) domain. This Roc–COR tandem is found in the...

The Rap-RapGAP complex: GTP hydrolysis without catalytic glutamine and arginine residues (2008)

Scrima, Andrea, Thomas, Christoph, Deaconescu, Delia, Wittinghofer, Alfred

The GAP arginine finger movement into the catalytic site of Ras increases the activation entropy (2008)

Kötting, Carsten, Kallenbach, Angela, Suveyzdis, Yan, Wittinghofer, Alfred, Gerwert, Klaus

The retinitis pigmentosa 2 gene product is a GTPase-activating protein for Arf-like 3 (2008)

Veltel, Stefan, Gasper, Raphael, Eisenacher, Elke, Wittinghofer, Alfred

Structure and function of Rho-type molecular switches in plants (2008)

Berken, Antje, Wittinghofer, Alfred

The Role of the Conserved Switch II Glutamate in Guanine Nucleotide Exchange Factor-Mediated Nucleotide Exchange of GTP-Binding Proteins (2008)

Gasper, Raphael, Thomas, Christoph, Ahmadian, Mohammad Reza, Wittinghofer, Alfred

The Crystal Structure of the Ran-Nup153ZnF2 Complex: a General Ran Docking Site at the Nuclear Pore Complex (2008)

Schrader, Nils, Koerner, Carolin, Koessmeier, Katja, Bangert, Jan Amadé, Wittinghofer, Alfred, Stoll, Raphael, ...

Specificity of Arl2/Arl3 signaling is mediated by a ternary Arl3-effector-GAP complex (2008)

Veltel, Stefan, Kravchenko, Aleksandra, Shehab, Ismail, Wittinghofer, Alfred

Fluoride complexes of oncogenic Ras mutants to study the Ras-RasGAP interaction (2008)

Gremer, Lothar, Gilsbach, Bernd, Ahmadian, Mohammad Reza, Wittinghofer, Alfred

Structure of the Roc-COR domain tandem of C. tepidum, a prokaryotic homologue of the human LRRK2 Parkinson kinase (2008)

Gotthardt, Katja, Weyand, Michael, Kortholt, Arjan, Van Haastert, Peter J. M., Wittinghofer, Alfred

Crystal Structures of the Conserved tRNA-Modifying Enzyme GidA: Implications for Its Interaction with MnmE and Substrate (2008)

Meyer, S, Scrima, Andrea, Versées, W, Wittinghofer, Alfred

Specificity of Interactions between mDia Isoforms and Rho Proteins (2008)

Lammers, Michael, Meyer, Simon, Kühlmann, Dorothee, Wittinghofer, Alfred

and (2007)

Andrea Friebel, Alfred Wittinghofer, Klaus Scheffzek, Max Von

The bacterial enteropathogen ######### # ########### employs a type III secretion system to inject bacterial toxins into the host cell cytosol. These toxins transiently activate Rho family...

Phosphorylation-independent interaction between 14-3-3 and exoenzyme S: from structure to pathogenesis (2007)

Christian Ottmann, Lubna Yasmin, Michael Weyand, Jeffrey L Veesenmeyer, Maureen H Diaz, Ruth H Palmer, ...

Capturing cyclic nucleotides in action: snapshots from crystallographic studies (2007)

Holger Rehmann, Alfred Wittinghofer, Johannes L. Bos

Structural Evidence for a Common Intermediate in Small G Protein-GEF Reactions (2007)

Thomas, Christoph, Fricke, Inka, Scrima, Andrea, Berken, Antje, Wittinghofer, Alfred

Capturing cyclic nucleotides in action: snapshots from crystallographic studies (2007)

Rehmann, Holger, Wittinghofer, Alfred, Bos, Johannes L.

Phosphorylation-independent interaction between 14-3-3 and exoenzyme S: from structure to pathogenesis (2007)

Ottmann, Christian, Yasmin, Lubna, Weyand, Michael, Veesenmeyer, Jeffrey L., Diaz, Maureen H., Palmer, Ruth H., ...

Insight into Catalysis of a Unique GTPase Reaction by a Combined Biochemical and FTIR Approach (2007)

Chakrabarti, Partha P., Daumke, Oliver, Suveyzdis, Yan, Kötting, Carsten, Gerwert, Klaus, Wittinghofer, Alfred

Structure of a 14-3-3 Coordinated Hexamer of the Plant Plasma Membrane H+-ATPase by Combining X-Ray Crystallography and Electron Cryomicroscopy (2007)

Ottmann, Christian, Marco, Sergio, Jaspert, Nina, Marcon, Caroline, Schauer, Nicolas, Weyand, Michael, ...

GEFs and GAPs: Critical Elements in the Control of Small G Proteins (2007)

Bos, Johannes L., Rehmann, Holger, Wittinghofer, Alfred

Human OLA1 Defines an ATPase Subfamily in the Obg Family of GTP-binding Proteins (2007)

Koller-Eichhorn, Roland, Marquardt, Tobias, Gail, Robert, Wittinghofer, Alfred, Kostrewa, Dirk, Kutay, Ulrike, ...

Structural insight into filament formation by mammalian septins (2007)

Sirajuddin, Minhajuddin, Farkasovsky, Marian, Hauer, Florian, Kühlmann, Dorothee, Macara, Ian G., Weyand, Michael, ...

How guanylate-binding proteins achieve assembly-stimulated processive cleavage of GTP to GMP (2006)

Ghosh,Agnidipta, Praefcke,Gerrit J. K., Renault,Louis, Wittinghofer,Alfred, Herrmann,Christian

Structure of the cyclic-AMP-responsive exchange factor Epac2 in its auto-inhibited state (2006)

Rehmann,Holger, Das,Joost, Knipscheer,Puck, Wittinghofer,Alfred, Bos,Johannes L

Structure of the cyclic-AMP-responsive exchange factor Epac2 in its auto-inhibited state (2006)

Holger Rehmann, Joost Das, Puck Knipscheer, Alfred Wittinghofer, Johannes L. Bos

Crystal Structure of the Human Retinitis Pigmentosa 2 Protein and Its Interaction with Arl3 (2006)

Kühnel,Karin, Veltel,Stefan, Schlichting,Ilme, Wittinghofer,Alfred

Characterization of the GbpD-activated Rap1 Pathway Regulating Adhesion and Cell Polarity in Dictyostelium discoideum (2006)

Kortholt, Arjan, Rehmann, Holger, Kae, Helmut, Bosgraaf, Leonard, Keizer-Gunnink, Ineke, Weeks, Gerald, ...

The regulation of cell polarity plays an important role in chemotaxis. GbpD, a putative nucleotide exchange factor for small G-proteins of the Ras family, has been implicated in adhesion, cell...

How guanylate-binding proteins achieve assembly-stimulated processive cleavage of GTP to GMP (2006)

Ghosh, Agnidipta, Praefcke, Gerrit J. K., Renault, Louis, Wittinghofer, Alfred, Herrmann, Christian

Crystal Structure of the Human Retinitis Pigmentosa 2 Protein and Its Interaction with Arl3 (2006)

Kühnel, Karin, Veltel, Stefan, Schlichting, Ilme, Wittinghofer, Alfred

Structure of the cyclic-AMP-responsive exchange factor Epac2 in its auto-inhibited state (2006)

Rehmann, Holger, Das, Joost, Knipscheer, Puck, Wittinghofer, Alfred, Bos, Johannes L

Phosphoryl transfer in Ras proteins, conclusive or elusive? (2006)

Wittinghofer, Alfred

GAP1 Family Members Constitute Bifunctional Ras and Rap GTPase-activating Proteins (2006)

Kupzig, Sabine, Deaconescu, Delia, Bouyoucef, Dalila, Walker, Simon A., Liu, Qing, Polte, Christian L., ...

Purification and crystallization of the catalytic PRONE domain of RopGEF8 and its complex with Rop4 from Arabidopsis thaliana (2006)

Thomas, Christoph, Weyand, Michael, Wittinghofer, Alfred, Berken, Antje

Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element (2006)

Scrima, Andrea, Wittinghofer, Alfred

A phosphoryl transfer intermediate in the GTPase reaction of Ras in complex with its GTPase-activating protein (2006)

Kötting, Carsten, Blessenohl, Marco, Suveyzdis, Yan, Goody, Roger S., Wittinghofer, Alfred, Gerwert, Klaus

Characterization of the GbpD-activated Rap1 Pathway Regulating Adhesion and Cell Polarity in Dictyostelium discoideum (2006)

Korholt, Arjan, Rehmann, Holger, Kae, Helmut, Bosgraaf, Leonard, Keizer-Gunnink, Ineke, Weeks, Gerald, ...

Structural Insights into HypB, a GTP-binding Protein That Regulates Metal Binding (2006)

Gasper, Raphael, Scrima, Andrea, Wittinghofer, Alfred

Lipidated Ras and Rab Peptides and Proteins - Synthesis, Structure, and Function (2006)

Brunsveld, Luc, Kuhlmann, Jürgen, Alexandrov, Kirill, Wittinghofer, Alfred, Goody, Roger S., Waldmann, Herbert

Lipidated Ras and Rab Peptides and Proteins - Synthesis, Structure, and Function (2006)

Brunsveld, Luc, Kuhlmann, Jürgen, Alexandrov, Kirill, Wittinghofer, Alfred, Goody, Roger S., Waldmann, Herbert

Lipidierte Ras- und Rab-Peptide und -Proteine: Synthese, Struktur und Funktion (2006)

Brunsveld, Luc, Kuhlmann, Jürgen, Alexandrov, Kirill, Wittinghofer, Alfred, Goody, Roger S., Waldmann, Herbert

Crystal Structure of the Human Retinitis Pigmentosa 2 Protein and Its Interaction with Arl3 (2006)

Kühnel, Karin, Veltel, Stefan, Schlichting, Ilme, Wittinghofer, Alfred

The crystal structure of human retinitis pigmentosa 2 protein (RP2) was solved to 2.1 Å resolution. It consists of an N−terminal β helix and a C−terminal ferredoxin−like α/β domain. RP2 is...

Lipidated Ras and Rab peptides and proteins: Synthesis, structure, and function (2006)

Brunsveld, Luc, Kuhlmann, Jürgen, Alexandrov, Kirill, Wittinghofer, Alfred, Goody, Roger, S., Waldmann, Herbert

Chemical biology can be defined as the study of biological phenomena from a chemical approach. Based on the analysis of relevant biological phenomena and their structural foundation, unsolved...

Lipidated ras and rab peptides and proteins: Synthesis, structure, and function (2006)

Brunsveld, Luc, Kuhlmann, Jürgen, Alexandrov, Kirill, Wittinghofer, Alfred, Goody, Roger S., Waldmann, Herbert

Chemical biology can be defined as the study of biological phenomena from a chemical approach. Based on the analysis of relevant biological phenomena and their structural foundation, unsolved...

Lipidated Ras and Rab peptides and proteins: Synthesis, structure, and function (2006)

Brunsveld, Luc, Kuhlmann, Jürgen, Alexandrov, Kirill, Wittinghofer, Alfred, Goody, Roger, S., Waldmann, Herbert

Chemical biology can be defined as the study of biological phenomena from a chemical approach. Based on the analysis of relevant biological phenomena and their structural foundation, unsolved...

Lipidated Ras and Rab peptides and proteins: Synthesis, structure, and function (2006)

Brunsveld, Luc, Kuhlmann, Jürgen, Alexandrov, Kirill, Wittinghofer, Alfred, Goody, Roger, S., Waldmann, Herbert

Chemical biology can be defined as the study of biological phenomena from a chemical approach. Based on the analysis of relevant biological phenomena and their structural foundation, unsolved...

A new family of RhoGEFs activates the Rop molecular switch in plants (2005)

Berken,Antje, Thomas,Christoph, Wittinghofer,Alfred

Nucleotide binding and filament assembly of recombinant yeast septin complexes (2005)

Farkasovsky,Marian, Herter,Peter, Voss,Beate, Wittinghofer,Alfred

A new family of RhoGEFs activates the Rop molecular switch in plants (2005)

Antje Berken, Christoph Thomas, Alfred Wittinghofer

Properties of the Interaction of Arf-like Protein 2 with PDEδ (2005)

Hanzal-Bayer,Michael, Linari,Marco, Wittinghofer,Alfred

Structural and mechanistic insights into the interaction between Rho and mammalian Dia (2005)

Rose,Rolf, Weyand,Michael, Lammers,Michael, Ishizaki,Toshimasa, Ahmadian,Mohammad Reza, Wittinghofer,Alfred

Crystal structure of THEP1 from the hyperthermophile Aquifex aeolicus: a variation of the RecA fold (2005)

Roßbach, Michael, Daumke, Oliver, Klinger, Claudia, Wittinghofer, Alfred, Kaufmann, Michael

Abstract Background aaTHEP1, the gene product of aq_1292 from Aquifex aeolicus , shows sequence homology to proteins from most thermophiles, hyperthermophiles, and higher organisms such as man,...

Crystal structure of THEP1 from the hyperthermophile Aquifex aeolicus: a variation of the RecA fold (2005)

Roßbach,Michael, Daumke,Oliver, Klinger,Claudia, Wittinghofer,Alfred, Kaufmann,Michael

An Acylation Cycle Regulates Localization and Activity of Palmitoylated Ras Isoforms (2005)

Rocks,Oliver, Peyker,Anna, Kahms,Martin, Verveer,Peter J., Koerner,Carolin, Lumbierres,Maria, ...

Systematic Peptide Array-based Delineation of the Differential β-Catenin Interaction with Tcf4, E-Cadherin, and Adenomatous Polyposis Coli (2005)

Gail,Robert, Frank,Ronald, Wittinghofer,Alfred

The purification and crystallization of mDia1 in complex with RhoC (2005)

Rose,Rolf, Wittinghofer,Alfred, Weyand,Michael

Conformational States of Ras Complexed with the GTP Analogue GppNHp or GppCH2p: Implications for the Interaction with Effector Proteins (2005)

Spoerner,Michael, Nuehs,Andrea, Ganser,Petra, Herrmann,Christian, Wittinghofer,Alfred, Kalbitzer,Hans Robert

The structure of the TrmE GTP-binding protein and its implications for tRNA modification (2005)

Scrima,Andrea, Vetter,Ingrid R., Armengod,M. Eugenia, Wittinghofer,Alfred

Systematic Peptide Array-based Delineation of the Differential β-Catenin Interaction with Tcf4, E-Cadherin, and Adenomatous Polyposis Coli (2005)

Gail, Robert, Frank, Ronald, Wittinghofer, Alfred

An Acylation Cycle Regulates Localization and Activity of Palmitoylated Ras Isoforms (2005)

Rocks, Oliver, Peyker, Anna, Kahms, Martin, Verveer, Peter J., Koerner, Carolin, Lumbierres, Maria, ...

Crystal structure of THEP1 from the hyperthermophile Aquifex aeolicus: a variation of the RecA fold (2005)

Roßbach, Michael, Daumke, Oliver, Klinger, Claudia, Wittinghofer, Alfred, Kaufmann, Michael

The structure of the TrmE GTP-binding protein and its implications for tRNA modification (2005)

Scrima, Andrea, Vetter, Ingrid R., Armengod, M. Eugenia, Wittinghofer, Alfred

Structural and mechanistic insights into the interaction between Rho and mammalian Dia (2005)

Rose, Rolf, Weyand, Michael, Lammers, Michael, Ishizaki, Toshimasa, Ahmadian, Mohammad Reza, Wittinghofer, Alfred

Conformational States of Ras Complexed with the GTP Analogue GppNHp or GppCH2p: Implications for the Interaction with Effector Proteins (2005)

Spoerner, Michael, Nuehs, Andrea, Ganser, Petra, Herrmann, Christian, Wittinghofer, Alfred, Kalbitzer, Hans Robert

A new family of RhoGEFs activates the Rop molecular switch in plants (2005)

Berken, Antje, Thomas, Christoph, Wittinghofer, Alfred

The purification and crystallization of mDia1 in complex with RhoC (2005)

Rose, Rolf, Wittinghofer, Alfred, Weyand, Michael

Nucleotide binding and filament assembly of recombinant yeast septin complexes (2005)

Farkasovsky, Marian, Herter, Peter, Voss, Beate, Wittinghofer, Alfred

Properties of the Interaction of Arf-like Protein 2 with PDEδ (2005)

Hanzal-Bayer, Michael, Linari, Marco, Wittinghofer, Alfred

The regulation of mDia1 by autoinhibition and its release by Rho●GTP (2005)

Lammers, Michael, Rose, Rolf, Scrima, Andrea, Wittinghofer, Alfred

An Acylation Cycle Regulates Localization and Activity of Palmitoylated Ras Isoforms (2005)

Rocks, Oliver, Peyker, Anna, Kahms, Martin, Verveer, Peter J., Koerner, Carolin, Lumbierres, Maria, ...

Properties of the Interaction of Arf-like Protein 2 with PDEδ (2005)

Hanzal-Bayer, Michael, Linari, Marco, Wittinghofer, Alfred

Arf-like proteins (Arl) share certain characteristic features with the Arf subfamily of Ras superfamily proteins, but their function is unknown. Here, we show by a variety of spectroscopic techniques...

Properties of the Interaction of Arf-like Protein 2 with PDEδ (2005)

Hanzal-Bayer, Michael, Linari, Marco, Wittinghofer, Alfred

Arf-like proteins (Arl) share certain characteristic features with the Arf subfamily of Ras superfamily proteins, but their function is unknown. Here, we show by a variety of spectroscopic techniques...

aeolicus: a variation of the RecA fold (2005)

Michael Roßbach, Oliver Daumke, Claudia Klinger, Alfred Wittinghofer, Michael Kaufmann

Crystal structure of THEP1 from the hyperthermophile Aquifex

Properties of the Interaction of Arf-like Protein 2 with PDEδ (2005)

Hanzal-Bayer, Michael, Linari, Marco, Wittinghofer, Alfred

Arf-like proteins (Arl) share certain characteristic features with the Arf subfamily of Ras superfamily proteins, but their function is unknown. Here, we show by a variety of spectroscopic techniques...

Properties of the Interaction of Arf-like Protein 2 with PDEδ (2005)

Hanzal-Bayer, Michael, Linari, Marco, Wittinghofer, Alfred

Arf-like proteins (Arl) share certain characteristic features with the Arf subfamily of Ras superfamily proteins, but their function is unknown. Here, we show by a variety of spectroscopic techniques...

The VASP tetramerization domain is a right-handed coiled coil based on a 15-residue repeat (2004)

Kühnel,Karin, Jarchau,Thomas, Wolf,Eva, Schlichting,Ilme, Ulrich,Walter, Wittinghofer,Alfred, ...

The VASP tetramerization domain is a right−handed coiled coil based on a 15−residue repeat (2004)

Kühnel,Karin, Jarchau,Thomas, Wolf,Eva, Schlichting,Ilme, Walter,Ulrich, Wittinghofer,Alfred, ...

The vasodilator−stimulated phosphoprotein (VASP) is a key regulator of actin dynamics. We have determined the 1.3−Å resolution crystal structure of the 45−residue−long tetramerization domain...

Perturbation of the conformational equilibria in Ras by selective mutations as studied by 31P NMR spectroscopy (2004)

Spoerner,Michael, Wittinghofer,Alfred, Kalbitzer,Hans Robert

Fourier Transform Infrared Spectroscopy on the Rap·RapGAP Reaction, GTPase Activation without an Arginine Finger (2004)

Chakrabarti,Partha P., Suveyzdis,Yan, Wittinghofer,Alfred, Gerwert,Klaus

Synthesis of GTP-Derived Ras Ligands (2004)

Soulère,Laurent, Aldrich,Courtney, Daumke,Oliver, Gail,Robert, Kissau,Lars, Wittinghofer,Alfred, ...

The GTPase-activating protein Rap1GAP uses a catalytic asparagine (2004)

Daumke,Oliver, Weyand,Michael, Chakrabarti,Partha P., Vetter,Ingrid R., Wittinghofer,Alfred

Purification, crystallization and preliminary structural characterization of human Rap1GAP (2004)

Daumke,Oliver, Wittinghofer,Alfred, Weyand,Michael

The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization (2004)

Shimada,Atsushi, Nyitrai,Miklós, Vetter,Ingrid R., Kühlmann,Dorothee, Bugyi,Beáta, Narumiya,Shuh, ...

The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization (2004)

Shimada, Atsushi, Nyitrai, Miklós, Vetter, Ingrid R., Kühlmann, Dorothee, Bugyi, Beáta, Narumiya, Shuh, ...

Purification, crystallization and preliminary structural characterization of human Rap1GAP (2004)

Daumke, Oliver, Wittinghofer, Alfred, Weyand, Michael

Fourier Transform Infrared Spectroscopy on the Rap·RapGAP Reaction, GTPase Activation without an Arginine Finger (2004)

Chakrabarti, Partha P., Suveyzdis, Yan, Wittinghofer, Alfred, Gerwert, Klaus

The GTPase-activating protein Rap1GAP uses a catalytic asparagine (2004)

Daumke, Oliver, Weyand, Michael, Chakrabarti, Partha P., Vetter, Ingrid R., Wittinghofer, Alfred

The VASP tetramerization domain is a right-handed coiled coil based on a 15-residue repeat (2004)

Kühnel, Karin, Jarchau, Thomas, Wolf, Eva, Schlichting, Ilme, Ulrich, Walter, Wittinghofer, Alfred, ...

Synthesis of GTP-Derived Ras Ligands (2004)

Soulère, Laurent, Aldrich, Courtney, Daumke, Oliver, Gail, Robert, Kissau, Lars, Wittinghofer, Alfred, ...

Perturbation of the conformational equilibria in Ras by selective mutations as studied by 31P NMR spectroscopy (2004)

Spoerner, Michael, Wittinghofer, Alfred, Kalbitzer, Hans Robert

The VASP tetramerization domain is a right−handed coiled coil based on a 15−residue repeat (2004)

Kühnel, Karin, Jarchau, Thomas, Wolf, Eva, Schlichting, Ilme, Walter, Ulrich, Wittinghofer, Alfred, ...

The vasodilator−stimulated phosphoprotein (VASP) is a key regulator of actin dynamics. We have determined the 1.3−Å resolution crystal structure of the 45−residue−long tetramerization domain...

Biochemical characterization of the Ran-RanBP1-RanGAP system: Are RanBP proteins and the acidic tail of RanGAP required for the Ran-RanGAP GTPase reaction? (2003)

Seewald,Michael J., Kraemer,Astrid, Farkasovsky,Marian, Körner,Carolin, Wittinghofer,Alfred, Vetter,Ingrid R.

Amino acids of the bacterial toxin SopE involved in G nucleotide exchange on Cdc42 (2003)

Schlumberger,Markus C., Friebel,Andrea, Buchwald,Gretel, Scheffzek,Klaus, Wittinghofer,Alfred, Hardt,Wolf-Dietrich

Communication between the regulatory and the catalytic region of the cAMP-responsive guanine nucleotide exchange factor Epac (2003)

Rehmann,Holger, Rüppel,Alma, Bos,Johannes L., Wittinghofer,Alfred

Characterisation of PDZ-GEFs, a family of guanine nucleotide exchange factors specific for Rap1 and Rap2 (2003)

Kuiperij,H. Bea, De Rooij,Johan, Rehmann,Holger, Van Triest,Miranda, Wittinghofer,Alfred, Bos,Johannes L., ...

PDZ-GEF1 (RA-GEF/nRapGEP/CNrasGEF) is a guanine nucleotide exchange factor (GEF) characterised by the presence of a PSD- 95/DlgA/ZO-1 (PDZ) domain, a Ras-association (RA) domain and a region related...

Structure and regulation of the cAMP-binding domains of Epac2 (2003)

Rehmann,Holger, Prakash,Balaji, Wolf,Eva, Rueppel,Alma, De Rooij,Johan, Bos,Johannes L., ...

Cyclic adenosine monophosphate (cAMP) is a universal second messenger that, in eukaryotes, was believed to act only on cAMP-dependent protein kinase A (PKA) and cyclic nucleotide- regulated ion...

Structural view of a fungal toxin acting on a 14-3-3 regulatory complex (2003)

Würtele,Martin, Jelich-Ottmann,Christian, Wittinghofer,Alfred, Oecking,Claudia

Ligand-mediated activation of the cAMP-responsive guanine nucleotide exchange factor Epac (2003)

Rehmann,Holger, Schwede,Frank, Døskeland,Stein O., Wittinghofer,Alfred, Bos,Johannes L.

Characterisation of PDZ-GEFs, a family of guanine nucleotide exchange factors specific for Rap1 and Rap2 (2003)

Kuiperij, H. Bea, De Rooij, Johan, Rehmann, Holger, Van Triest, Miranda, Wittinghofer, Alfred, Bos, Johannes L., ...

PDZ-GEF1 (RA-GEF/nRapGEP/CNrasGEF) is a guanine nucleotide exchange factor (GEF) characterised by the presence of a PSD- 95/DlgA/ZO-1 (PDZ) domain, a Ras-association (RA) domain and a region related...

Structure and regulation of the cAMP-binding domains of Epac2 (2003)

Rehmann, Holger, Prakash, Balaji, Wolf, Eva, Rueppel, Alma, De Rooij, Johan, Bos, Johannes L., ...

Cyclic adenosine monophosphate (cAMP) is a universal second messenger that, in eukaryotes, was believed to act only on cAMP-dependent protein kinase A (PKA) and cyclic nucleotide- regulated ion...

Structural view of a fungal toxin acting on a 14-3-3 regulatory complex (2003)

Würtele, Martin, Jelich-Ottmann, Christian, Wittinghofer, Alfred, Oecking, Claudia

Biochemical characterization of the Ran-RanBP1-RanGAP system: Are RanBP proteins and the acidic tail of RanGAP required for the Ran-RanGAP GTPase reaction? (2003)

Seewald, Michael J., Kraemer, Astrid, Farkasovsky, Marian, Körner, Carolin, Wittinghofer, Alfred, Vetter, Ingrid R.

Amino acids of the bacterial toxin SopE involved in G nucleotide exchange on Cdc42 (2003)

Schlumberger, Markus C., Friebel, Andrea, Buchwald, Gretel, Scheffzek, Klaus, Wittinghofer, Alfred, Hardt, Wolf-Dietrich

Communication between the regulatory and the catalytic region of the cAMP-responsive guanine nucleotide exchange factor Epac (2003)

Rehmann, Holger, Rüppel, Alma, Bos, Johannes L., Wittinghofer, Alfred

Ligand-mediated activation of the cAMP-responsive guanine nucleotide exchange factor Epac (2003)

Rehmann, Holger, Schwede, Frank, Døskeland, Stein O., Wittinghofer, Alfred, Bos, Johannes L.

Structure and regulation of the cAMP-binding domains of Epac2 (2002)

Holger Rehmann, Balaji Prakash, Eva Wolf, Alma Rueppel, Johan De Rooij, Johannes L. Bos, ...

Proteins - from little acorns mighty oak trees grow - Editorial overview (2002)

Baker,Edward N., Wittinghofer,Alfred

Fluorescently labelled guanine nucleotide binding proteins to analyse elementary steps of GAP-catalysed reactions (2002)

Kraemer,Astrid, Brinkmann,Thilo, Plettner,Ina, Goody,Roger, Wittinghofer,Alfred

Downregulation of small guanine nucleotide-binding proteins (GNBPs) requires the interaction with their corresponding GTPase-activating proteins (GAPs), which increase the slow intrinsic GTPase...

Patent: Technique and Screening Method for Detecting Reversible Protein-Protein Interactions (2002)

Wittinghofer,Alfred, Rocks,Oliver

The invention relates to methods and assays for detecting protein-protein interactions and for testing active substances in expression systems such as cells. According to the invention, protein I...

Structural basis for the reversible activation of a Rho protein by the bacterial toxin SopE (2002)

Buchwald,Gretel, Friebel,Andrea, Galán,Jorge E., Hardt,Wolf-Dietrich, Wittinghofer,Alfred, Scheffzek,Klaus

The bacterial enteropathogen Salmonella typhimurium employs a type III secretion system to inject bacterial toxins into the host cell cytosol. These toxins transiently activate Rho family GTP-binding...

The complex of Arl2-GTP and PDEδ: from structure to function (2002)

Hanzal-Bayer,Michael, Renault,Louis, Roversi,Pietro, Wittinghofer,Alfred, Hillig,Roman C.

Arf-like (Arl) proteins are close relatives of the Arf regulators of vesicular transport, but their function is unknown. Here, we present the crystal structure of full-length Arl2-GTP in complex with...

Rap-specific GTPase activating protein follows an alternative mechanism (2002)

Brinkmann,Thilo, Daumke,Oliver, Herbrand,Ulrike, Kühlmann,Dorothee, Stege,Patricia, Ahmadian,Mohammad Reza, ...

Rap1 is a small GTPase that is involved in signal transduction cascades. It is highly homologous to Ras but it is down- regulated by its own set of GTPase activating proteins (GAPs). To investigate...

The actin filament architecture: tightly regulated by the cells, manipulated by pathogens (2002)

Ahmadian,Mohammad Reza, Wittinghofer,Alfred, Schmidt,Gudula

RanGAP mediates GTP hydrolysis without an arginine finger (2002)

Seewald,Michael J., Körner,Carolin, Wittinghofer,Alfred, Vetter,Ingrid R.

GTPase-activating proteins (GAPs) increase the rate of GTP hydrolysis on guanine nucleotide-binding proteins by many orders of magnitude. Studies with Ras and Rho have elucidated the mechanism of GAP...

RanGAP mediates GTP hydrolysis without an arginine finger (2002)

Michael J. Seewald, Carolin Körner, Alfred Wittinghofer, Ingrid R. Vetter

Fluorescence Methods in the Study of Small GTP-Binding Proteins (2002)

Ahmadian,Mohammad Reza, Wittinghofer,Alfred, Herrmann,Christian

The actin filament architecture: tightly regulated by the cells, manipulated by pathogens (2002)

Ahmadian,Mohammad Reza, Wittinghofer,Alfred, Schmidt,Gudula

Proteins - from little acorns mighty oak trees grow - Editorial overview (2002)

Baker, Edward N., Wittinghofer, Alfred

Fluorescently labelled guanine nucleotide binding proteins to analyse elementary steps of GAP-catalysed reactions (2002)

Kraemer, Astrid, Brinkmann, Thilo, Plettner, Ina, Goody, Roger, Wittinghofer, Alfred

Downregulation of small guanine nucleotide-binding proteins (GNBPs) requires the interaction with their corresponding GTPase-activating proteins (GAPs), which increase the slow intrinsic GTPase...

RanGAP mediates GTP hydrolysis without an arginine finger (2002)

Seewald, Michael J., Körner, Carolin, Wittinghofer, Alfred, Vetter, Ingrid R.

GTPase-activating proteins (GAPs) increase the rate of GTP hydrolysis on guanine nucleotide-binding proteins by many orders of magnitude. Studies with Ras and Rho have elucidated the mechanism of GAP...

Fluorescence Methods in the Study of Small GTP-Binding Proteins (2002)

Ahmadian, Mohammad Reza, Wittinghofer, Alfred, Herrmann, Christian

Obg, a G domain with a beautiful extension (2002)

Wittinghofer, Alfred

The structure of Obg, a protein involved in a complicated genetic network that regulates stress response and sporulation in Bacillus subtilis, reveals a completely new type of guanine nucleotide...

Patent: Technique and Screening Method for Detecting Reversible Protein-Protein Interactions (2002)

Wittinghofer, Alfred, Rocks, Oliver

The invention relates to methods and assays for detecting protein-protein interactions and for testing active substances in expression systems such as cells. According to the invention, protein I...

Rap-specific GTPase activating protein follows an alternative mechanism (2002)

Brinkmann, Thilo, Daumke, Oliver, Herbrand, Ulrike, Kühlmann, Dorothee, Stege, Patricia, Ahmadian, Mohammad Reza, ...

Rap1 is a small GTPase that is involved in signal transduction cascades. It is highly homologous to Ras but it is down- regulated by its own set of GTPase activating proteins (GAPs). To investigate...

Structural basis for the reversible activation of a Rho protein by the bacterial toxin SopE (2002)

Buchwald, Gretel, Friebel, Andrea, Galán, Jorge E., Hardt, Wolf-Dietrich, Wittinghofer, Alfred, Scheffzek, Klaus

The bacterial enteropathogen Salmonella typhimurium employs a type III secretion system to inject bacterial toxins into the host cell cytosol. These toxins transiently activate Rho family GTP-binding...

The complex of Arl2-GTP and PDEδ: from structure to function (2002)

Hanzal-Bayer, Michael, Renault, Louis, Roversi, Pietro, Wittinghofer, Alfred, Hillig, Roman C.

Arf-like (Arl) proteins are close relatives of the Arf regulators of vesicular transport, but their function is unknown. Here, we present the crystal structure of full-length Arl2-GTP in complex with...

Diverse mechanims for GTP hydrolysis by GTP-binding proteins (2002)

Wittinghofer, Alfred

The actin filament architecture: tightly regulated by the cells, manipulated by pathogens (2002)

Ahmadian, Mohammad Reza, Wittinghofer, Alfred, Schmidt, Gudula

The actin filament architecture: tightly regulated by the cells, manipulated by pathogens (2002)

Ahmadian, Mohammad Reza, Wittinghofer, Alfred, Schmidt, Gudula

The complex of Arl2-GTP and PDE[delta]: from structure to function (2002)

Hanzal-Bayer, Michael, Renault, Louis, Roversi, Pietro, Wittinghofer, Alfred, Hillig, Roman C.

rf-like (Arl) proteins are close relatives of the Arf regulators of vesicular transport, but their function is unknown. Here, we present the crystal structure of full-length Arl2-GTP in complex with...

The complex of Arl2-GTP and PDE[delta]: from structure to function (2002)

Hanzal-Bayer, Michael, Renault, Louis, Roversi, Pietro, Wittinghofer, Alfred, Hillig, Roman C.

rf-like (Arl) proteins are close relatives of the Arf regulators of vesicular transport, but their function is unknown. Here, we present the crystal structure of full-length Arl2-GTP in complex with...

The complex of Arl2-GTP and PDE[delta]: from structure to function (2002)

Hanzal-Bayer, Michael, Renault, Louis, Roversi, Pietro, Wittinghofer, Alfred, Hillig, Roman C.

rf-like (Arl) proteins are close relatives of the Arf regulators of vesicular transport, but their function is unknown. Here, we present the crystal structure of full-length Arl2-GTP in complex with...

The complex of Arl2-GTP and PDE[delta]: from structure to function (2002)

Hanzal-Bayer, Michael, Renault, Louis, Roversi, Pietro, Wittinghofer, Alfred, Hillig, Roman C.

rf-like (Arl) proteins are close relatives of the Arf regulators of vesicular transport, but their function is unknown. Here, we present the crystal structure of full-length Arl2-GTP in complex with...

The Ras-Byr2RBD Complex: Structural Basis for Ras Effector Recognition in Yeast (2001)

Scheffzek,Klaus, Grünewald,Petra, Wohlgemuth,Sabine, Kabsch,Wolfgang, Tu,Hua, Wigler,Mike, ...

The small GTP binding protein Ras has important roles in cellular growth and differentiation. Mutant Ras is permanently active and contributes to cancer development. In its activated form, Ras...

How the Pseudomonas aeruginosa ExoS toxin downregulates Rac (2001)

Martin Würtele, Eva Wolf, Kristin J. Pederson, Gretel Buchwald, Mohammad Reza Ahmadian, Joseph T. Barbieri, ...

Monitoring the GAP catalyzed H-Ras GTPase reaction at atomic resolution in real time. (2001)

Allin,Christoph, Ahmadian,Mohammad Reza, Wittinghofer,Alfred, Gerwert,Klaus

The Ras-Byr2RBD Complex: Structural Basis for Ras Effector Recognition in Yeast (2001)

Scheffzek, Klaus, Grünewald, Petra, Wohlgemuth, Sabine, Kabsch, Wolfgang, Tu, Hua, Wigler, Mike, ...

The small GTP binding protein Ras has important roles in cellular growth and differentiation. Mutant Ras is permanently active and contributes to cancer development. In its activated form, Ras...

Monitoring the GAP catalyzed H-Ras GTPase reaction at atomic resolution in real time. (2001)

Allin, Christoph, Ahmadian, Mohammad Reza, Wittinghofer, Alfred, Gerwert, Klaus

Structure of human guanylate-binding protein 1 representing a unique class of GTP-binding proteins (2000)

Balaji Prakash, Gerrit J. K. Praefcke, Louis Renault, Alfred Wittinghofer, Christian Herrmann

Bioorganic synthesis of lipid-modified proteins for the study of signal transduction (2000)

Benjamin Bader, Karsten Kuhn, David J. Owen, Herbert Waldmann, Alfred Wittinghofer, Jürgen Kuhlmann

Structural and biochemical properties show ARL3-GDP as a distinct GTP binding protein (2000)

Hillig, Roman C., Hanzal-Bayer, Michael, Linari, Marco, Becker, Jörg, Wittinghofer, Alfred, Renault, Louis

Structural and biochemical properties show ARL3-GDP as a distinct GTP binding protein (2000)

Hillig, Roman C., Hanzal-Bayer, Michael, Linari, Marco, Becker, Jörg, Wittinghofer, Alfred, Renault, Louis

Structural and biochemical properties show ARL3-GDP as a distinct GTP binding protein (2000)

Hillig, Roman C., Hanzal-Bayer, Michael, Linari, Marco, Becker, Jörg, Wittinghofer, Alfred, Renault, Louis

Conformational states of the nuclear GTP-binding protein Ran and its complexes with the exchange factor RCC1 and the effector protein RanBP1 (1999)

Geyer,Matthias, Assheuer,Ralf, Klebe,Christian, Kuhlmann,Jürgen, Becker,Jörg, Wittinghofer,Alfred, ...

It has been shown before by (31)P NMR that Ras bound to the nonhydrolyzable GTP analogue guanosine 5-O-(beta, gamma-imidotriphosphate) (GppNHp) exists in two conformations which are rapidly...

Guanosine triphosphatase stimulation of oncogenic Ras mutants (1999)

Ahmadian,Mohammad Reza, Zor,Tsaffrir, Vogt,Dorothee, Kabsch,Wolfgang, Selinger,Zvi, Wittinghofer,Alfred, ...

Interest in the guanosine triphosphatase (GTPase) reaction of Ras as a molecular drug target stems from the observation that, in a large number of human tumors, Ras is characteristically mutated at...

Guanosine triphosphatase stimulation of oncogenic Ras mutants (1999)

Ahmadian,Mohammad Reza, Zor,Tsaffrir, Vogt,Dorothee, Kabsch,Wolfgang, Selinger,Zvi, Wittinghofer,Alfred, ...

Interest in the guanosine triphosphatase (GTPase) reaction of Ras as a molecular drug target stems from the observation that, in a large number of human tumors, Ras is characteristically mutated at...

Guanosine triphosphatase stimulation of oncogenic Ras mutants (1999)

Ahmadian, Mohammad Reza, Zor, Tsaffrir, Vogt, Dorothee, Kabsch, Wolfgang, Selinger, Zvi, Wittinghofer, Alfred, ...

Interest in the guanosine triphosphatase (GTPase) reaction of Ras as a molecular drug target stems from the observation that, in a large number of human tumors, Ras is characteristically mutated at...

Conformational states of the nuclear GTP-binding protein Ran and its complexes with the exchange factor RCC1 and the effector protein RanBP1 (1999)

Geyer, Matthias, Assheuer, Ralf, Klebe, Christian, Kuhlmann, Jürgen, Becker, Jörg, Wittinghofer, Alfred, ...

It has been shown before by (31)P NMR that Ras bound to the nonhydrolyzable GTP analogue guanosine 5-O-(beta, gamma-imidotriphosphate) (GppNHp) exists in two conformations which are rapidly...

Guanosine triphosphatase stimulation of oncogenic Ras mutants (1999)

Ahmadian, Mohammad Reza, Zor, Tsaffrir, Vogt, Dorothee, Kabsch, Wolfgang, Selinger, Zvi, Wittinghofer, Alfred, ...

Interest in the guanosine triphosphatase (GTPase) reaction of Ras as a molecular drug target stems from the observation that, in a large number of human tumors, Ras is characteristically mutated at...

Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP (1998)

Johan De Rooij, Fried J. T. Zwartkruis, Mark H. G. Verheijen, Robbert H. Cool, Sebastian M. B. Nijman, Alfred Wittinghofer, ...

Structural analysis of the GAP-related domain from neurofibromin and its implications (1998)

Scheffzek,Klaus, Ahmadian,Mohammad Reza, Wiesmüller,Lisa, Kabsch,Wolfgang, Stege,Patricia, Schmitz,Frank, ...

Neurofibromin is the product of the NF1 gene, whose alteration is responsible for the pathogenesis of neurofibromatosis type 1 (NF1), one of the most frequent genetic disorders in man. It acts as a...

Structural analysis of the GAP-related domain from neurofibromin and its implications (1998)

Klaus Scheffzek, Mohammad Reza Ahmadian, Lisa Wiesmüller, Wolfgang Kabsch, Patricia Stege, Frank Schmitz, ...

GTPase-activating proteins: helping hands to complement an active site (1998)

Scheffzek,Klaus, Ahmadian,Mohammad Reza, Wittinghofer,Alfred

Structural analysis of the GAP-related domain from neurofibromin and its implications (1998)

Scheffzek, Klaus, Ahmadian, Mohammad Reza, Wiesmüller, Lisa, Kabsch, Wolfgang, Stege, Patricia, Schmitz, Frank, ...

Neurofibromin is the product of the NF1 gene, whose alteration is responsible for the pathogenesis of neurofibromatosis type 1 (NF1), one of the most frequent genetic disorders in man. It acts as a...

GTPase-activating proteins: helping hands to complement an active site (1998)

Scheffzek, Klaus, Ahmadian, Mohammad Reza, Wittinghofer, Alfred

Aluminium fluoride associates with the small guanine nucleotide binding proteins (1997)

Ahmadian,Mohammad Reza, Mittal,Rohit, Hall,Alan, Wittinghofer,Alfred

Individual rate constants for the interaction of Ras proteins with GTPase-activating proteins determined by fluorescence spectroscopy (1997)

Ahmadian,Mohammad Reza, Hoffmann,Ulrike, Goody,Roger S., Wittinghofer,Alfred

Individual rate constants for the interaction of H-, K-, and N-Ras with GAP-334 and NF1-333 were determined using fluorescent derivatives of guanine nucleotides at the active site of the Ras...

Individual rate constants for the interaction of Ras proteins with GTPase-activating proteins determined by fluorescence spectroscopy (1997)

Ahmadian, Mohammad Reza, Hoffmann, Ulrike, Goody, Roger S., Wittinghofer, Alfred

Individual rate constants for the interaction of H-, K-, and N-Ras with GAP-334 and NF1-333 were determined using fluorescent derivatives of guanine nucleotides at the active site of the Ras...

Signaling mechanistics: Aluminum fluoride for molecule of the year (1997)

Wittinghofer, Alfred

Recent three-dimensional structures of phosphoryl transfer enzymes in their aluminum fluoride bound state and corresponding biochemical data have shown how diverse biological problems can be...

Aluminium fluoride associates with the small guanine nucleotide binding proteins (1997)

Ahmadian, Mohammad Reza, Mittal, Rohit, Hall, Alan, Wittinghofer, Alfred

Confirmation of the arginine-finger hypothesis for the GAP-stimulated GTP-hydrolysis reaction of Ras (1997)

Ahmadian, Mohammad Reza, Stege, Patricia, Scheffzek, Klaus, Wittinghofer, Alfred

Crystal structure of the GTPase-activating domain of human p120GAP and implications for the interaction with Ras (1996)

Klaus Scheffzek, Alfred Lautwein, Wolfgang Kabsch, Mohammad Reza Ahmadian, Alfred Wittinghofer

Crystal structure of the GTPase-activating domain of human p120GAP and implications for the interaction with Ras (1996)

Scheffzek,Klaus, Lautwein,A., Kabsch,Wolfgang, Ahmadian,Mohammad Reza, Wittinghofer,Alfred

Interactions between Ras proteins and their effectors (1996)

McCormick,Frank, Wittinghofer,Alfred

Conformational transitions in p21ras and in its complexes with the effector protein Raf-RBD and the GTPase activating protein GAP (1996)

Geyer,Matthias, Schweins,Thomas, Herrmann,Christian, Prisner,Thomas, Wittinghofer,Alfred, Kalbitzer,Hans Robert

Crystal structure of the GTPase-activating domain of human p120GAP and implications for the interaction with Ras (1996)

Scheffzek, Klaus, Lautwein, A., Kabsch, Wolfgang, Ahmadian, Mohammad Reza, Wittinghofer, Alfred

Deciphering the alphabet of G proteins: The structure of the α, β, γ heterotrimer (1996)

Wittinghofer, Alfred

Interactions between Ras proteins and their effectors (1996)

McCormick, Frank, Wittinghofer, Alfred

Conformational transitions in p21ras and in its complexes with the effector protein Raf-RBD and the GTPase activating protein GAP (1996)

Geyer, Matthias, Schweins, Thomas, Herrmann, Christian, Prisner, Thomas, Wittinghofer, Alfred, Kalbitzer, Hans Robert

Conserved Histidine Residues of RCC1 Are Essential for Nucleotide Exchange on Ran (1996)

Azuma, Yoshiaki, Seino, Hiroaki, Seki, Takashi, Uzawa, Satoru, Klebe, Christian, Ohba, Tomoyuki, ...

Charged amino acid residues of human RCC1 were converted to alanine and mutants which were unable to complement tsBN2 cells (a temperature-sensitive rccl− mutant of the hamster BHK21 cell line)...

The 2.2 Å crystal structure of the Ras-binding domain of the serine/threonine kinase c-Raf1 in complex with RaplA and a GTP analogue (1995)

Nicolas Nassar, Gudrun Horn, Christian A. Herrmann, Anna Scherer, Frank McCormick, Alfred Wittinghofer

Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form (1995)

Scheffzek,Klaus, Klebe,Christian, Fritz-Wolf,Karin, Kabsch,Wolfgang, Wittinghofer,Alfred

The Ran proteins constitute a distinct branch of the superfamily of Ras-related GTP-binding proteins which function as molecular switches cycling between GTP-bound on and GDP-bound off states. Ran is...

Substrate and product structural requirements for binding of nucleotides to H-ras p21: the mechanism of discrimination between guanosine and adenosine nucleotides (1995)

Rensland,H., John,J., Linke,R., Simon,I., Schlichting,Ilme, Wittinghofer,Alfred, ...

The interaction of the protein product of the H-ras oncogene with a series of nucleoside di- and triphosphates has been examined to investigate the tolerance of the active site to departures from the...

Substrate and product structural requirements for binding of nucleotides to H-ras p21: the mechanism of discrimination between guanosine and adenosine nucleotides (1995)

Rensland, H., John, J., Linke, R., Simon, I., Schlichting, Ilme, Wittinghofer, Alfred, ...

The interaction of the protein product of the H-ras oncogene with a series of nucleoside di- and triphosphates has been examined to investigate the tolerance of the active site to departures from the...

Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form (1995)

Scheffzek, Klaus, Klebe, Christian, Fritz-Wolf, Karin, Kabsch, Wolfgang, Wittinghofer, Alfred

The Ran proteins constitute a distinct branch of the superfamily of Ras-related GTP-binding proteins which function as molecular switches cycling between GTP-bound on and GDP-bound off states. Ran is...

A Universal Expression–Purification System Based on the Coiled–Coil Interaction of Myosin Heavy Chain (1992)

Vera Wolber, Kayo Maeda, Renate Schumann, Birgit Brandmeier, Lisa Wiesmüller, Alfred Wittinghofer

p21 with a phenylalanine 28-leucine mutation reacts normally with the GTPase activating protein GAP but nevertheless has transforming properties (1991)

Reinstein,Jochen, Schlichting,Ilme, Frech,M., Goody,Roger S., Wittinghofer,Alfred

The H-ras gene product p21H has been mutated at Phe-28, which makes a hydrophobic interaction with the guanine base of bound GDP/GTP. The mutation Phe-28----Leu drastically increases nucleotide...

p21 with a phenylalanine 28-leucine mutation reacts normally with the GTPase activating protein GAP but nevertheless has transforming properties (1991)

Reinstein, Jochen, Schlichting, Ilme, Frech, M., Goody, Roger S., Wittinghofer, Alfred

The H-ras gene product p21H has been mutated at Phe-28, which makes a hydrophobic interaction with the guanine base of bound GDP/GTP. The mutation Phe-28----Leu drastically increases nucleotide...

Three-dimensional studies of H-ras p21 mutants: molecular basis of their inability to function as signal switches (1990)

Krengel,U., Schlichting,Ilme, Scherer,Anna, Schumann,Renate, Frech,M., John,J., ...

Time-resolved X-ray crystallographic study of the conformational change in Ha-ras p21 protein on GTP hydrolysis (1990)

Schlichting,Ilme, Almo,S. C., Rapp,G., Wilson,K., Petratos,K., Lentfer,A., ...

Three-dimensional studies of H-ras p21 mutants: molecular basis of their inability to function as signal switches (1990)

Krengel,U., Schlichting,Ilme, Scherer,Anna, Schumann,Renate, Frech,M., John,J., ...

Time-resolved X-ray crystallographic study of the conformational change in Ha-ras p21 protein on GTP hydrolysis (1990)

Schlichting,Ilme, Almo,S. C., Rapp,G., Wilson,K., Petratos,K., Lentfer,A., ...

Three-dimensional studies of H-ras p21 mutants: molecular basis of their inability to function as signal switches (1990)

Krengel, U., Schlichting, Ilme, Scherer, Anna, Schumann, Renate, Frech, M., John, J., ...

Time-resolved X-ray crystallographic study of the conformational change in Ha-ras p21 protein on GTP hydrolysis (1990)

Schlichting, Ilme, Almo, S. C., Rapp, G., Wilson, K., Petratos, K., Lentfer, A., ...

Three-dimensional studies of H-ras p21 mutants: molecular basis of their inability to function as signal switches (1990)

Krengel, U., Schlichting, Ilme, Scherer, Anna, Schumann, Renate, Frech, M., John, J., ...

Time-resolved X-ray crystallographic study of the conformational change in Ha-ras p21 protein on GTP hydrolysis (1990)

Schlichting, Ilme, Almo, S. C., Rapp, G., Wilson, K., Petratos, K., Lentfer, A., ...

Structure of the guanine-nucleotide-binding domain of the Ha-ras oncogene product p21 in the triphosphate conformation (1989)

Emil F. Pai, Wolfgang Kabsch, Ute Krengel, Kenneth C. Holmes, Jacob John, Alfred Wittinghofer

Structure of the guanine-nucleotide-binding domain of the Ha-ras oncogene product p21 in the triphosphate conformation (1989)

Pai,Emil F., Kabsch,Wolfgang, Krengel,U., Holmes,Kenneth C., Wray,John, Wittinghofer,Alfred

Structural and catalytic role of arginine 88 in Escherichia coli adenylate kinase as evidenced by chemical modification and site- directed mutagenesis (1989)

Reinstein,Jochen, Gilles,A. M., Rose,T., Wittinghofer,Alfred, Saint Girons,I., Barzu,O., ...

Phenylglyoxal inactivates Escherichia coli adenylate kinase by modifying a single arginine residue (Arg-88). ATP, ADP, P1,P5- di(adenosine 5)-pentaphosphate, and to a lesser extent AMP protect the...

Crystallization and preliminary X-ray analysis of the human c-H-ras- oncogene product p21 complexed with GTP analogues (1989)

Scherer,Anna, John,J., Linke,R., Goody,Roger S., Wittinghofer,Alfred, Pai,Emil F., ...

Structure of the guanine-nucleotide-binding domain of the Ha-ras oncogene product p21 in the triphosphate conformation (1989)

Pai, Emil F., Kabsch, Wolfgang, Krengel, U., Holmes, Kenneth C., Wray, John, Wittinghofer, Alfred

Structural and catalytic role of arginine 88 in Escherichia coli adenylate kinase as evidenced by chemical modification and site- directed mutagenesis (1989)

Reinstein, Jochen, Gilles, A. M., Rose, T., Wittinghofer, Alfred, Saint Girons, I., Barzu, O., ...

Phenylglyoxal inactivates Escherichia coli adenylate kinase by modifying a single arginine residue (Arg-88). ATP, ADP, P1,P5- di(adenosine 5)-pentaphosphate, and to a lesser extent AMP protect the...

Crystallization and preliminary X-ray analysis of the human c-H-ras- oncogene product p21 complexed with GTP analogues (1989)

Scherer, Anna, John, J., Linke, R., Goody, Roger S., Wittinghofer, Alfred, Pai, Emil F., ...

ras p21 protein promotes survival and fiber outgrowth of cultured embryonic neurons (1989)

Borasio, Gian Domenico, John, Jacob, Wittinghofer, Alfred, Barde, Yves-Alain, Sendtner, Michael, Heumann, Rolf

Although evidence obtained with the PC12 cell line has suggested a role for the ras oncogene proteins in the signal transduction of nerve growth factor-mediated fiber outgrowth, little is known about...

An efficient expression vector (pPLEX) for bacterial synthesis of non-fusion proteins and protein domains (1987)

Sczakiel, Georg, Wittinghofer, Alfred, Tucker, Jane

31P NMR studies on ATP.Mg2+p21-nucleotide complexes. Chemical shifts, rate and equilibrium constants (1986)

Klaus,W., Schlichting,Ilme, Goody,Roger S., Wittinghofer,Alfred, Rosch,P., Holmes,Kenneth C.

31P NMR studies on ATP.Mg2+p21-nucleotide complexes. Chemical shifts, rate and equilibrium constants (1986)

Klaus,W., Schlichting,Ilme, Goody,Roger S., Wittinghofer,Alfred, Rosch,P., Holmes,Kenneth C.

31P NMR studies on ATP.Mg2+p21-nucleotide complexes. Chemical shifts, rate and equilibrium constants (1986)

Klaus, W., Schlichting, Ilme, Goody, Roger S., Wittinghofer, Alfred, Rosch, P., Holmes, Kenneth C.

31P NMR studies on ATP.Mg2+p21-nucleotide complexes. Chemical shifts, rate and equilibrium constants (1986)

Klaus, W., Schlichting, Ilme, Goody, Roger S., Wittinghofer, Alfred, Rosch, P., Holmes, Kenneth C.

Crystals of a large tryptic peptide (fragment A) of elongation factor EF-Tu from Escherichia coli (1977)

Gast,W. H., Kabsch,Wolfgang, Wittinghofer,Alfred, Lebermann,Reuben

We have recently described the crystallisation of native EF-Tu.GDP from E. coli [ 1 ] and of the product obtained by mild tryptic digestion of this protein [2] using polyethylene glycol (PEG) 6000 as...

Crystals of a large tryptic peptide (fragment A) of elongation factor EF-Tu from Escherichia coli (1977)

Gast, W. H., Kabsch, Wolfgang, Wittinghofer, Alfred, Lebermann, Reuben

We have recently described the crystallisation of native EF-Tu.GDP from E. coli [ 1 ] and of the product obtained by mild tryptic digestion of this protein [2] using polyethylene glycol (PEG) 6000 as...

Zur Synthese der Schafinsulin-A-Kette mit 6-11-Disulfidring / (1971)

Wittinghofer, Alfred.

Thesis (doctoral)--Technische Hochschule Aachen, 1971.

Guanosine triphosphatase stimulation of oncogenic Ras mutants

Ahmadian, Mohammad Reza, Zor, Tsaffrir, Vogt, Dorothee, Kabsch, Wolfgang, Selinger, Zvi, Wittinghofer, Alfred, ...

Interest in the guanosine triphosphatase (GTPase) reaction of Ras as a molecular drug target stems from the observation that, in a large number of human tumors, Ras is characteristically mutated at...

Dynamic properties of the Ras switch I region and its importance for binding to effectors

Spoerner, Michael, Herrmann, Christian, Vetter, Ingrid R., Kalbitzer, Hans Robert, Wittinghofer, Alfred

We have investigated the dynamic properties of the switch I region of the GTP-binding protein Ras by using mutants of Thr-35, an invariant residue necessary for the switch function. Here we show that...

Monitoring the GAP catalyzed H-Ras GTPase reaction at atomic resolution in real time

Allin, Christoph, Ahmadian, Mohammad Reza, Wittinghofer, Alfred, Gerwert, Klaus

The molecular reaction mechanism of the GTPase-activating protein (GAP)-catalyzed GTP hydrolysis by Ras was investigated by time resolved Fourier transform infrared (FTIR) difference spectroscopy...

The Ras Mutant D119N Is Both Dominant Negative and Activated

Cool, Robbert H., Schmidt, Gudula, Lenzen, Christian U., Prinz, Heino, Vogt, Dorothee, Wittinghofer, Alfred

The introduction of mutation D119N (or its homolog) in the NKxD nucleotide binding motif of various Ras-like proteins produces constitutively activated or dominant-negative effects, depending on the...

Conformational Switch and Role of Phosphorylation in PAK Activation

Buchwald, Gretel, Hostinova, Eva, Rudolph, Markus G., Kraemer, Astrid, Sickmann, Albert, Meyer, Helmut E., ...

p21-activated protein kinases (PAKs) are involved in signal transduction processes initiating a variety of biological responses. They become activated by interaction with Rho-type small GTP-binding...

The RafC1 Cysteine-Rich Domain Contains Multiple Distinct Regulatory Epitopes Which Control Ras-Dependent Raf Activation

Daub, Martina, Jöckel, Johannes, Quack, Thomas, Weber, Christoph K., Schmitz, Frank, Rapp, Ulf R., ...

Activation of c-Raf-1 (referred to as Raf) by Ras is a pivotal step in mitogenic signaling. Raf activation is initiated by binding of Ras to the regulatory N terminus of Raf. While Ras binding to...

The complex of Arl2-GTP and PDEδ: from structure to function

Hanzal-Bayer, Michael, Renault, Louis, Roversi, Pietro, Wittinghofer, Alfred, Hillig, Roman C.

Arf-like (Arl) proteins are close relatives of the Arf regulators of vesicular transport, but their function is unknown. Here, we present the crystal structure of full-length Arl2-GTP in complex with...

Structural basis for the reversible activation of a Rho protein by the bacterial toxin SopE

Buchwald, Gretel, Friebel, Andrea, Galán, Jorge E., Hardt, Wolf-Dietrich, Wittinghofer, Alfred, Scheffzek, Klaus

The bacterial enteropathogen Salmonella typhimurium employs a type III secretion system to inject bacterial toxins into the host cell cytosol. These toxins transiently activate Rho family GTP-binding...

Structural view of a fungal toxin acting on a 14-3-3 regulatory complex

Würtele, Martin, Jelich-Ottmann, Christian, Wittinghofer, Alfred, Oecking, Claudia

The fungal phytotoxin fusicoccin stabilizes the interaction between the C-terminus of the plant plasma membrane H+-ATPase and 14-3-3 proteins, thus leading to permanent activation of the proton pump....

Biochemical Characterization of the Ran-RanBP1-RanGAP System: Are RanBP Proteins and the Acidic Tail of RanGAP Required for the Ran-RanGAP GTPase Reaction?

Seewald, Michael J., Kraemer, Astrid, Farkasovsky, Marian, Körner, Carolin, Wittinghofer, Alfred, Vetter, Ingrid R.

RanBP type proteins have been reported to increase the catalytic efficiency of the RanGAP-mediated GTPase reaction on Ran. Since the structure of the Ran-RanBP1-RanGAP complex showed RanBP1 to be...

Triphosphate structure of guanylate-binding protein 1 and implications for nucleotide binding and GTPase mechanism

Prakash, Balaji, Renault, Louis, Praefcke, Gerrit J.K., Herrmann, Christian, Wittinghofer, Alfred

The interferon-γ-induced guanylate-binding protein 1 (GBP1) belongs to a special class of large GTP- binding proteins of 60–100 kDa with unique characteristics. Here we present the structure of...

The VASP tetramerization domain is a right-handed coiled coil based on a 15-residue repeat

Kühnel, Karin, Jarchau, Thomas, Wolf, Eva, Schlichting, Ilme, Walter, Ulrich, Wittinghofer, Alfred, ...

The vasodilator-stimulated phosphoprotein (VASP) is a key regulator of actin dynamics. We have determined the 1.3-Å resolution crystal structure of the 45-residue-long tetramerization domain (TD)...

The structure of the TrmE GTP-binding protein and its implications for tRNA modification

Scrima, Andrea, Vetter, Ingrid R, Armengod, M Eugenia, Wittinghofer, Alfred

TrmE is a 50 kDa guanine nucleotide-binding protein conserved between bacteria and man. It is involved in the modification of uridine bases (U34) at the first anticodon (wobble) position of tRNAs...

Crystal structure of THEP1 from the hyperthermophile Aquifex aeolicus: a variation of the RecA fold

Roßbach, Michael, Daumke, Oliver, Klinger, Claudia, Wittinghofer, Alfred, Kaufmann, Michael

The actin filament architecture: tightly regulated by the cells, manipulated by pathogens: International Titisee Conference on the actin cytoskeleton: from signalling to bacterial pathogenesis

Ahmadian, Mohammad Reza, Wittinghofer, Alfred, Schmidt, Gudula

The regulation of mDia1 by autoinhibition and its release by Rho•GTP

Lammers, Michael, Rose, Rolf, Scrima, Andrea, Wittinghofer, Alfred

Formins induce the nucleation and polymerisation of unbranched actin filaments via the formin-homology domains 1 and 2. Diaphanous-related formins (Drfs) are regulated by a RhoGTPase-binding domain...

Guanosine triphosphatase stimulation of oncogenic Ras mutants

Ahmadian, Mohammad Reza, Zor, Tsaffrir, Vogt, Dorothee, Kabsch, Wolfgang, Selinger, Zvi, Wittinghofer, Alfred, ...

Interest in the guanosine triphosphatase (GTPase) reaction of Ras as a molecular drug target stems from the observation that, in a large number of human tumors, Ras is characteristically mutated at...

Dynamic properties of the Ras switch I region and its importance for binding to effectors

Spoerner, Michael, Herrmann, Christian, Vetter, Ingrid R., Kalbitzer, Hans Robert, Wittinghofer, Alfred

We have investigated the dynamic properties of the switch I region of the GTP-binding protein Ras by using mutants of Thr-35, an invariant residue necessary for the switch function. Here we show that...

Monitoring the GAP catalyzed H-Ras GTPase reaction at atomic resolution in real time

Allin, Christoph, Ahmadian, Mohammad Reza, Wittinghofer, Alfred, Gerwert, Klaus

The molecular reaction mechanism of the GTPase-activating protein (GAP)-catalyzed GTP hydrolysis by Ras was investigated by time resolved Fourier transform infrared (FTIR) difference spectroscopy...

The Ras Mutant D119N Is Both Dominant Negative and Activated

Cool, Robbert H., Schmidt, Gudula, Lenzen, Christian U., Prinz, Heino, Vogt, Dorothee, Wittinghofer, Alfred

The introduction of mutation D119N (or its homolog) in the NKxD nucleotide binding motif of various Ras-like proteins produces constitutively activated or dominant-negative effects, depending on the...

Conformational Switch and Role of Phosphorylation in PAK Activation

Buchwald, Gretel, Hostinova, Eva, Rudolph, Markus G., Kraemer, Astrid, Sickmann, Albert, Meyer, Helmut E., ...

p21-activated protein kinases (PAKs) are involved in signal transduction processes initiating a variety of biological responses. They become activated by interaction with Rho-type small GTP-binding...

The RafC1 Cysteine-Rich Domain Contains Multiple Distinct Regulatory Epitopes Which Control Ras-Dependent Raf Activation

Daub, Martina, Jöckel, Johannes, Quack, Thomas, Weber, Christoph K., Schmitz, Frank, Rapp, Ulf R., ...

Activation of c-Raf-1 (referred to as Raf) by Ras is a pivotal step in mitogenic signaling. Raf activation is initiated by binding of Ras to the regulatory N terminus of Raf. While Ras binding to...

The complex of Arl2-GTP and PDEδ: from structure to function

Hanzal-Bayer, Michael, Renault, Louis, Roversi, Pietro, Wittinghofer, Alfred, Hillig, Roman C.

Arf-like (Arl) proteins are close relatives of the Arf regulators of vesicular transport, but their function is unknown. Here, we present the crystal structure of full-length Arl2-GTP in complex with...

Structural basis for the reversible activation of a Rho protein by the bacterial toxin SopE

Buchwald, Gretel, Friebel, Andrea, Galán, Jorge E., Hardt, Wolf-Dietrich, Wittinghofer, Alfred, Scheffzek, Klaus

The bacterial enteropathogen Salmonella typhimurium employs a type III secretion system to inject bacterial toxins into the host cell cytosol. These toxins transiently activate Rho family GTP-binding...

Structural view of a fungal toxin acting on a 14-3-3 regulatory complex

Würtele, Martin, Jelich-Ottmann, Christian, Wittinghofer, Alfred, Oecking, Claudia

The fungal phytotoxin fusicoccin stabilizes the interaction between the C-terminus of the plant plasma membrane H+-ATPase and 14-3-3 proteins, thus leading to permanent activation of the proton pump....

Biochemical Characterization of the Ran-RanBP1-RanGAP System: Are RanBP Proteins and the Acidic Tail of RanGAP Required for the Ran-RanGAP GTPase Reaction?

Seewald, Michael J., Kraemer, Astrid, Farkasovsky, Marian, Körner, Carolin, Wittinghofer, Alfred, Vetter, Ingrid R.

RanBP type proteins have been reported to increase the catalytic efficiency of the RanGAP-mediated GTPase reaction on Ran. Since the structure of the Ran-RanBP1-RanGAP complex showed RanBP1 to be...

Triphosphate structure of guanylate-binding protein 1 and implications for nucleotide binding and GTPase mechanism

Prakash, Balaji, Renault, Louis, Praefcke, Gerrit J.K., Herrmann, Christian, Wittinghofer, Alfred

The interferon-γ-induced guanylate-binding protein 1 (GBP1) belongs to a special class of large GTP- binding proteins of 60–100 kDa with unique characteristics. Here we present the structure of...

The VASP tetramerization domain is a right-handed coiled coil based on a 15-residue repeat

Kühnel, Karin, Jarchau, Thomas, Wolf, Eva, Schlichting, Ilme, Walter, Ulrich, Wittinghofer, Alfred, ...

The vasodilator-stimulated phosphoprotein (VASP) is a key regulator of actin dynamics. We have determined the 1.3-Å resolution crystal structure of the 45-residue-long tetramerization domain (TD)...

The structure of the TrmE GTP-binding protein and its implications for tRNA modification

Scrima, Andrea, Vetter, Ingrid R, Armengod, M Eugenia, Wittinghofer, Alfred

TrmE is a 50 kDa guanine nucleotide-binding protein conserved between bacteria and man. It is involved in the modification of uridine bases (U34) at the first anticodon (wobble) position of tRNAs...

Crystal structure of THEP1 from the hyperthermophile Aquifex aeolicus: a variation of the RecA fold

Roßbach, Michael, Daumke, Oliver, Klinger, Claudia, Wittinghofer, Alfred, Kaufmann, Michael

The actin filament architecture: tightly regulated by the cells, manipulated by pathogens: International Titisee Conference on the actin cytoskeleton: from signalling to bacterial pathogenesis

Ahmadian, Mohammad Reza, Wittinghofer, Alfred, Schmidt, Gudula

The regulation of mDia1 by autoinhibition and its release by Rho•GTP

Lammers, Michael, Rose, Rolf, Scrima, Andrea, Wittinghofer, Alfred

Formins induce the nucleation and polymerisation of unbranched actin filaments via the formin-homology domains 1 and 2. Diaphanous-related formins (Drfs) are regulated by a RhoGTPase-binding domain...

A phosphoryl transfer intermediate in the GTPase reaction of Ras in complex with its GTPase-activating protein

Kötting, Carsten, Blessenohl, Marco, Suveyzdis, Yan, Goody, Roger S., Wittinghofer, Alfred, Gerwert, Klaus

The hydrolysis of nucleoside triphosphates by enzymes is used as a regulation mechanism in key biological processes. Here, the GTP hydrolysis of the protein complex of Ras with its GTPase-activating...

Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element

Scrima, Andrea, Wittinghofer, Alfred

MnmE, a Guanine nucleotide-binding protein conserved between bacteria and man, is involved in the modification of tRNAs. Here we provide biochemical and X-ray structural evidence for a new...

The purification and crystallization of mDia1 in complex with RhoC

Rose, Rolf, Wittinghofer, Alfred, Weyand, Michael

An N-terminal construct of mDia1, a member of the diaphanous-related formin family, was overexpressed, purified and crystallized in complex with RhoC. Data collection from native and SeMet crystals...

Phosphorylation-independent interaction between 14-3-3 and exoenzyme S: from structure to pathogenesis

Ottmann, Christian, Yasmin, Lubna, Weyand, Michael, Veesenmeyer, Jeffrey L, Diaz, Maureen H, Palmer, Ruth H, ...

14-3-3 proteins are phosphoserine/phosphothreonine-recognizing adapter proteins that regulate the activity of a vast array of targets. There are also examples of 14-3-3 proteins binding their targets...

Purification and crystallization of the catalytic PRONE domain of RopGEF8 and its complex with Rop4 from Arabidopsis thaliana

Thomas, Christoph, Weyand, Michael, Wittinghofer, Alfred, Berken, Antje

Crystals of the catalytic PRONE domain of the guanine nucleotide exchange factor RopGEF8 and its complex with the Rho-family protein Rop4 from A. thaliana were obtained that diffract to 2.2 and...

The GAP arginine finger movement into the catalytic site of Ras increases the activation entropy

Kötting, Carsten, Kallenbach, Angela, Suveyzdis, Yan, Wittinghofer, Alfred, Gerwert, Klaus

Members of the Ras superfamily of small G proteins play key roles in signal transduction pathways, which they control by GTP hydrolysis. They are regulated by GTPase activating proteins (GAPs)....

The Rap–RapGAP complex: GTP hydrolysis without catalytic glutamine and arginine residues

Scrima, Andrea, Thomas, Christoph, Deaconescu, Delia, Wittinghofer, Alfred

The GTP-binding protein Rap1 regulates integrin-mediated and other cell adhesion processes. Unlike most other Ras-related proteins, it contains a threonine in switch II instead of a glutamine (Gln61...

Structure of the Roc–COR domain tandem of C. tepidum, a prokaryotic homologue of the human LRRK2 Parkinson kinase

Gotthardt, Katja, Weyand, Michael, Kortholt, Arjan, Van Haastert, Peter J M, Wittinghofer, Alfred

Correction to: The EMBO Journal (2008) 27, 2239–2249. doi:10.1038/emboj.2008.150

Structure of the Roc–COR domain tandem of C. tepidum, a prokaryotic homologue of the human LRRK2 Parkinson kinase

Gotthardt, Katja, Weyand, Michael, Kortholt, Arjan, Van Haastert, Peter J M, Wittinghofer, Alfred

Ras of complex proteins (Roc) belongs to the superfamily of Ras-related small G-proteins that always occurs in tandem with the C-terminal of Roc (COR) domain. This Roc–COR tandem is found in the...

Kissing G Domains of MnmE Monitored by X-Ray Crystallography and Pulse Electron Paramagnetic Resonance Spectroscopy

Meyer, Simon, Böhme, Sabine, Krüger, André, Steinhoff, Heinz-Jürgen, Klare, Johann P., Wittinghofer, Alfred

The authors of this research article demonstrate the nature of the conformational changes MnmE was previously suggested to undergo during its GTPase cycle, and show the nucleotide-dependent dynamic...

GTP-induced conformational changes in septins and implications for function

Sirajuddin, Minhajuddin, Farkasovsky, Marian, Zent, Eldar, Wittinghofer, Alfred

Septins constitute a group of GTP-binding proteins involved in cytokinesis and other essential cellular functions. They form heterooligomeric complexes that polymerize into nonpolar filaments and are...

A Receptor-associated Protein/Phosphatidylinositol 3-Kinase Pathway Controls Pseudopod Formation

Kortholt, Arjan, Bolourani, Parvin, Rehmann, Holger, Keizer-Gunnink, Ineke, Weeks, Gerald, Wittinghofer, Alfred, ...

GbpD, a guanine exchange factor specific for Rap1, has been implicated in adhesion, cell polarity, and chemotaxis of Dictyostelium cells. Here it is shown that activated Rap1 directly binds to PI3K....

Alfred Wittinghofer

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