Sequence-specific binding of Taz1p dimers to fission yeast telomeric DNA (2000)
Spink, Karen G., Evans, Rebecca J., Chambers, Alistair
The fission yeast (Schizosaccharomyces pombe) taz1 gene encodes a telomere-associated protein. It contains a single copy of a Myb-like motif termed the telobox that is also found in the human...
Graham, Ian R., Chambers, Alistair
We have used the technique known as selected and amplified binding (SAAB) to Isolate binding sites for the yeast transcription factor RAP1 from a degenerate pool of oligonucleotldes. A total of 47...
Characterisation of the DNA binding domain of the yeast RAP1 protein (1990)
Henry, Yves A.L., Chambers, Alistair, Tsang, Jimmy S.H., Kingsman, Alan J., Kingsman, Susan M.
The 827 amino acid yeast RAP1 protein interacts with DNA to regulate gene expression at numerous unrelated loci in the yeast genome. By a combination of amino, carboxy and internal deletions, we have...
Chambers, Alistair, Stanway, Clive, Tsang, Jimmy S.H., Henry, Yves, Kingsman, Alan J., Kingsman, Susan M.
The UAS of the yeast gene encoding the glycolytic enzyme phosphoglycerate kinase (PGK) contains several different sequence elements involved in transcriptional activation. These elements include the...
Tsang, Jimmy S.H., Henry, Yves A.L., Chambers, Alistair, Kingsman, Alan J., Kingsman, Susan M.
Yeast repressor activator protein 1 (RAP 1) binds in vitro to specific DNA sequences that are found in diverse genetic elements. Expression of the yeast phosphoglycerate kinase gene (PGK) requires...
Stanway, Clive A., Chambers, Alistair, Kingsman, Alan J., Kingsman, Susan M.
The upstream activator (UAS) of the yeast PGK gene comprises three different sequence elements. These are 1) a region of strong protein binding called the YFP, 2) three repeats of the motif CTTCC and...
The UAS of the yeast PGK gene is composed of multiple functional elements (1988)
Chambers, Alistair, Stanway, Clive, Kingsman, Alan J., Kingsman, Susan M.
The UAS ( upstream activator sequence ) of the yeast PGK gene contains a transcriptional activator domain located between bases −479 and −402 upstream from the initiating ATG. This region of the...
In Vivo Analysis of Functional Regions within Yeast Rap1p
Graham, Ian R., Haw, Robin A., Spink, Karen G., Halden, Kathryn A., Chambers, Alistair
We have analyzed the in vivo importance of different regions of Rap1p, a yeast transcriptional regulator and telomere binding protein. A yeast strain (SCR101) containing a regulatable RAP1 gene was...
Sequence-specific binding of Taz1p dimers to fission yeast telomeric DNA
Spink, Karen G., Evans, Rebecca J., Chambers, Alistair
The fission yeast (Schizosaccharomyces pombe) taz1 gene encodes a telomere-associated protein. It contains a single copy of a Myb-like motif termed the telobox that is also found in the human...
In Vivo Analysis of Functional Regions within Yeast Rap1p
Graham, Ian R., Haw, Robin A., Spink, Karen G., Halden, Kathryn A., Chambers, Alistair
We have analyzed the in vivo importance of different regions of Rap1p, a yeast transcriptional regulator and telomere binding protein. A yeast strain (SCR101) containing a regulatable RAP1 gene was...
Sequence-specific binding of Taz1p dimers to fission yeast telomeric DNA
Spink, Karen G., Evans, Rebecca J., Chambers, Alistair
The fission yeast (Schizosaccharomyces pombe) taz1 gene encodes a telomere-associated protein. It contains a single copy of a Myb-like motif termed the telobox that is also found in the human...