Evidence for DNA Translocation by the ISWI Chromatin-Remodeling Enzyme
Whitehouse, Iestyn, Stockdale, Chris, Flaus, Andrew, Szczelkun, Mark D., Owen-Hughes, Tom
The ISWI proteins form the catalytic core of a subset of ATP-dependent chromatin-remodeling activities. Here, we studied the interaction of the ISWI protein with nucleosomal substrates. We found that...
Dynamic Properties of Nucleosomes during Thermal and ATP-Driven Mobilization
Flaus, Andrew, Owen-Hughes, Tom
The fundamental subunit of chromatin, the nucleosome, is not a static entity but can move along DNA via either thermal or enzyme-driven movements. Here we have monitored the movements of nucleosomes...
Sin mutations alter inherent nucleosome mobility
Flaus, Andrew, Rencurel, Chantal, Ferreira, Helder, Wiechens, Nicola, Owen-Hughes, Tom
Previous studies have identified sin mutations that alleviate the requirement for the yeast SWI/SNF chromatin remodelling complex, which include point changes in the yeast genes encoding core...
Identification of multiple distinct Snf2 subfamilies with conserved structural motifs
Flaus, Andrew, Martin, David M. A., Barton, Geoffrey J., Owen-Hughes, Tom
The Snf2 family of helicase-related proteins includes the catalytic subunits of ATP-dependent chromatin remodelling complexes found in all eukaryotes. These act to regulate the structure and dynamic...
Dürr, Harald, Flaus, Andrew, Owen-Hughes, Tom, Hopfner, Karl-Peter
Proteins with sequence similarity to the yeast Snf2 protein form a large family of ATPases that act to alter the structure of a diverse range of DNA–protein structures including chromatin. Snf2...
Evidence for DNA Translocation by the ISWI Chromatin-Remodeling Enzyme
Whitehouse, Iestyn, Stockdale, Chris, Flaus, Andrew, Szczelkun, Mark D., Owen-Hughes, Tom
The ISWI proteins form the catalytic core of a subset of ATP-dependent chromatin-remodeling activities. Here, we studied the interaction of the ISWI protein with nucleosomal substrates. We found that...
Dynamic Properties of Nucleosomes during Thermal and ATP-Driven Mobilization
Flaus, Andrew, Owen-Hughes, Tom
The fundamental subunit of chromatin, the nucleosome, is not a static entity but can move along DNA via either thermal or enzyme-driven movements. Here we have monitored the movements of nucleosomes...
Sin mutations alter inherent nucleosome mobility
Flaus, Andrew, Rencurel, Chantal, Ferreira, Helder, Wiechens, Nicola, Owen-Hughes, Tom
Previous studies have identified sin mutations that alleviate the requirement for the yeast SWI/SNF chromatin remodelling complex, which include point changes in the yeast genes encoding core...
Identification of multiple distinct Snf2 subfamilies with conserved structural motifs
Flaus, Andrew, Martin, David M. A., Barton, Geoffrey J., Owen-Hughes, Tom
The Snf2 family of helicase-related proteins includes the catalytic subunits of ATP-dependent chromatin remodelling complexes found in all eukaryotes. These act to regulate the structure and dynamic...
Dürr, Harald, Flaus, Andrew, Owen-Hughes, Tom, Hopfner, Karl-Peter
Proteins with sequence similarity to the yeast Snf2 protein form a large family of ATPases that act to alter the structure of a diverse range of DNA–protein structures including chromatin. Snf2...
Histone Tails and the H3 αN Helix Regulate Nucleosome Mobility and Stability▿
Ferreira, Helder, Somers, Joanna, Webster, Ryan, Flaus, Andrew, Owen-Hughes, Tom
Nucleosomes fulfill the apparently conflicting roles of compacting DNA within eukaryotic genomes while permitting access to regulatory factors. Central to this is their ability to stably associate...
Ferreira, Helder, Flaus, Andrew, Owen-Hughes, Tom
Alteration of chromatin structure by chromatin modifying and remodelling activities is a key stage in the regulation of many nuclear processes. These activities are frequently interlinked, and many...