Andrew JG Simpson

Identification of unannotated exons of low abundance transcripts in Drosophila melanogasterand cloning of a new serine protease gene upregulated upon injury (2007)

Maia, Rafaela M, Valente, Valeria, Cunha, Marco AV, Sousa, Josane F, Araujo, Daniela D, Silva, Wilson A, ...

Abstract Background The sequencing of the D.melanogaster genome revealed an unexpected small number of genes (~ 14,000) indicating that mechanisms acting on generation of transcript diversity must...

Analysis of the First and Second Internal Transcribed Spacer Sequences of the Ribosomal DNA in Biomphalaria tenagophila   Complex (Mollusca: Planorbidae) (2004)

Teofânia HDA Vidigal Linus Spatz, Jessica C Kissinger, Rodrigo AF Redondo, Edina CR Pires, Andrew JG Simpson, Omar S Carvalho

The first and second internal transcribed spacer regions (ITS1 and ITS2) of the ribosomal DNA of Biomphalaria tenagophila   complex (B. tenagophila, B. occidentalis, and B. t. guaibensis) were...

Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae) (2004)

Vidigal,Teofânia HDA, Spatz,Linus, Kissinger,Jessica C, Redondo,Rodrigo AF, Pires,Edina CR, Simpson,Andrew JG, ...

The first and second internal transcribed spacer regions (ITS1 and ITS2) of the ribosomal DNA of Biomphalaria tenagophila complex (B. tenagophila, B. occidentalis, and B. t. guaibensis) were...

Polymerase Chain Reaction and Restriction Fragment Length Polymorphism of Cytocrome Oxidase Subunit I Used for Differentiation of Brazilian Biomphalaria   Species Intermediate Host of Schistosoma mansoni   (2002)

Teofânia HDA Vidigal, Lângia C Montresor, Andrew JG Simpson, Omar S Carvalho

The intermediate hosts of Schistosoma mansoni   , in Brazil, Biomphalaria glabrata   , B. tenagophila and B. straminea , were identified by restriction fragment length polymorphism analysis of the...

Polymerase chain reaction and restriction fragment length polymorphism of cytocrome oxidase subunit I used for differentiation of Brazilian Biomphalaria species intermediate host of Schistosoma mansoni (2002)

Vidigal,Teofânia HDA, Montresor,Lângia C, Simpson,Andrew JG, Carvalho,Omar S

The intermediate hosts of Schistosoma mansoni, in Brazil, Biomphalaria glabrata, B. tenagophila and B. straminea, were identified by restriction fragment length polymorphism analysis of the...

Genetic Variability in Brazilian Populations of Biomphalaria straminea Complex Detected by Simple Sequence Repeat Anchored Polymerase Chain Reaction Amplification (2001)

Roberta L Caldeira, Teofânia HDA Vidigal, Andrew JG Simpson, Omar S Carvalho

Biomphalaria glabrata, B. tenagophila and B. straminea are intermediate hosts of Schistosoma mansoni, in Brazil. The latter is of epidemiological importance in the northwest of Brazil and, due to...

Identification of Biomphalaria havanensis and Biomphalaria obstructa Populations from Cuba Using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism of the Ribosomal RNA Intergenic Spacer (2001)

Teofânia HDA Vidigal, Roberta L Caldeira, Andrew JG Simpson, Omar S Carvalho

In Cuba, several Biomphalaria species have been reported such as B. orbignyi, B. schrammi, B. helophila, B. havanensis and B. peregrina; only the latter three are considered as potential hosts of...

Identification of Biomphalaria havanensis and Biomphalaria obstructa populations from Cuba using polymerase chain reaction and restriction fragment length polymorphism of the ribosomal RNA intergenic spacer (2001)

Vidigal,Teofânia HDA, Caldeira,Roberta L, Simpson,Andrew JG, Carvalho,Omar S

In Cuba, several Biomphalaria species have been reported such as B. orbignyi, B. schrammi, B. helophila, B. havanensis and B. peregrina; only the latter three are considered as potential hosts of...

Genetic variability in Brazilian populations of Biomphalaria straminea complex detected by simple sequence repeat anchored polymerase chain reaction amplification (2001)

Caldeira,Roberta L, Vidigal,Teofânia HDA, Simpson,Andrew JG, Carvalho,Omar S

Biomphalaria glabrata, B. tenagophila and B. straminea are intermediate hosts of Schistosoma mansoni, in Brazil. The latter is of epidemiological importance in the northwest of Brazil and, due to...

Further Studies on the Molecular Systematics of Biomphalaria Snails from Brazil (2000)

Teofânia HDA Vidigal, Roberta Lima Caldeira, Andrew JG Simpson, Omar S Carvalho

The polymerase chain reaction and restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) region of the rRNA gene, using the enzyme DdeI were used for the molecular...

Identification of Planorbids from Venezuela by Polymerase Chain Reaction Amplification and Restriction Fragment Length Polymorphism of Internal Transcriber Spacer of the RNA Ribosomal Gene (2000)

Roberta L Caldeira, Teofânia HDA Vidigal, Liboria Matinella, Andrew JG Simpson, Omar S Carvalho

Snails of the genus Biomphalaria from Venezuela were subjected to morphological assessment as well as polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis....

Identification of planorbids from Venezuela by polymerase chain reaction amplification and restriction fragment length polymorphism of internal transcriber spacer of the RNA ribosomal gene (2000)

Caldeira,Roberta L, Vidigal,Teofânia HDA, Matinella,Liboria, Simpson,Andrew JG, Carvalho,Omar S

Snails of the genus Biomphalaria from Venezuela were subjected to morphological assessment as well as polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis....

Further studies on the molecular systematics of Biomphalaria snails from Brazil (2000)

Vidigal,Teofânia HDA, Caldeira,Roberta Lima, Simpson,Andrew JG, Carvalho,Omar S

The polymerase chain reaction and restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) region of the rRNA gene, using the enzyme DdeI were used for the molecular...

RESEARCH NOTE - The Tegument of Schistosoma mansoni: Genes, Antigens and the Host-Parasite Relationship (1998)

Edneide M Xavier, Norma Lucena-Silva, Roberto P Werkhauser, Gloria R Franco, Ricardo AA Lira Santos, Andrew JG Simpson, ...

Cercaria, the infective larvae of Schistosoma mansoni are released from a molluscan host and must penetrate the skin of the human host within several hours in order to continue the life-cycle of the...

Genetic Variability and Identification of the Intermediate Snail Hosts of Schistosoma mansoni (1998)

Teofânia HDA Vidigal, Emmanuel Dias Neto, Linus Spatz, Diana N Nunes, Edina R Pires, Andrew JG Simpson, ...

Studies based on shell or reproductive organ morphology and genetic considerations suggest extensive intraspecific variation in Biomphalaria snails. The high variability at the morphological and...

Molecular Identification of Similar Species of the Genus Biomphalaria (Mollusca: Planorbidae) Determined by a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (1998)

Roberta Lima Caldeira, Teofânia HDA Vidigal, Sônia Torquato Paulinelli, Andrew JG Simpson, Omar S Carvalho

The freshwater snails Biomphalaria straminea, B. intermedia, B. kuhniana and B. peregrina, are morphologically similar; based on this similarity the first three species were therefore grouped in the...

Genetic variability and identification of the intermediate snail hosts of Schistosoma mansoni (1998)

Vidigal,Teofânia HDA, Dias Neto,Emmanuel, Spatz,Linus, Nunes,Diana N, Pires,Edina R, Simpson,Andrew JG, ...

Studies based on shell or reproductive organ morphology and genetic considerations suggest extensive intraspecific variation in Biomphalaria snails. The high variability at the morphological and...

Molecular identification of similar species of the genus Biomphalaria (Mollusca: Planorbidae) determined by a polymerase chain reaction-restriction fragment length polymorphism (1998)

Caldeira,Roberta Lima, Vidigal,Teofânia HDA, Paulinelli,Sônia Torquato, Simpson,Andrew JG, Carvalho,Omar S

The freshwater snails Biomphalaria straminea, B. intermedia, B. kuhniana and B. peregrina, are morphologically similar; based on this similarity the first three species were therefore grouped in the...

The tegument of Schistosoma mansoni: genes, antigens and the host-parasite relationship (1998)

Xavier,Edneide M, Lucena-Silva,Norma, Werkhauser,Roberto P, Franco,Gloria R, Santos,Ricardo AA Lira, Simpson,Andrew JG, ...

Specific identification of Biomphalaria tenagophila and Biomphalaria occidentalis populations by the low stringency polymerase chain reaction (1997)

Edina Rodrigues Pires, Teofania HDA Vidigal, Horacio MS Teles, Andrew JG Simpson, Omar S Carvalho

Although Biomphalaria occidentalis and B. tenagophila are indistinguishable on the basis of shell morphology and the majority of their genital organs, only the latter is susceptible to infection with...

Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni (1997)

Patricia Machado Pinto, Cristiana FA Brito, Liana K Jannoti Passos, Miriam Tendler, Andrew JG Simpson

The extent of genomic variability of clones of Schistosoma mansoni obtained from field isolates was compared with that of strains that have been laboratory maintained. Analysis was undertaken using...

Evidentiation of Paramyosin (Sm-97) as a modulating antigen on granulomatous hypersensitivity to Schistosoma mansoni eggs (1997)

Cristine Hirsch, Claudia Carvalho-Queiroz, Gloria R Franco, Sergio DJ Pena, Andrew JG Simpson, Alfredo M Goes

A Schistosoma mansoni adult worm anionic fraction (PIII) has previously been shown to protect mice against challenge infection and to reduce pulmonary and hepatic granulomatous hypersensitivity....

The Schistosome Genome Project: RNA Arbitrarily Primed- PCR Allows the Accelerated Generation of Expressed Sequence Tags (1996)

Emmanuel Dias Neto, Richard Harrop, Rodrigo Correa-Oliveira, Sergio DJ Pena, R Alan Wilson, Andrew JG Simpson

A Low Stringency Polymerase Chain Reaction Approach to the Identification of Biomphalaria glabrata and B. tenagophila, Intermediate Snail Hosts of Schistosoma mansoni in Brazil (1996)

Teofania HDA Vidigal, Emmanuel Dias Neto, Andrew JG Simpson, Omar S Carvalho

The low stringency-polymerase chain reaction (LS-PCR) with a pair of specific primers for the amplification of the 18S rRNA gene was evaluated as a means of differentiating between the two...

DNA Polymorphism of Schistosomes and their Snail Hosts (1995)

Andrew JG Simpson, Emmanuel Dias Neto, Teofania Hda Vidigal, Heloisa B Pena, Omar S Carvalho, Sergio DJ Pena

Analysis of the genomes of schistosomes and one of their intermediate hosts, Biomphalaria glabrata, using Random Amplified Polymorphic DNA (RAPD) demonstrated that intraspecific genetic polymorphism...

Sequencing and Identification of Expressed Schistosoma mansoni   Genes by Random Selection of cDNA Clones from a Directional Library (1995)

Gloria R Franco, Andrew JG Simpson, Sergio DJ Pena

We have initiated a gene discovery program in Schistosoma mansoni   based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic...

Genomics and the renaissance of generalism

Simpson, Andrew JG

A meeting report of the sessions on human, eukaryotic and bacterial genome sequencing at the American Society for Microbiology and Institut Pasteur joint conference: Genomes 2000 International...

Sequence-based genomics

Simpson, Andrew JG

A report on the Genome-Based Pathogen Biology meeting, Hinxton, UK, 7-10 July 2002.

The use of Open Reading frame ESTs (ORESTES) for analysis of the honey bee transcriptome

Nunes, Francis MF, Valente, Valeria, Sousa, Josane F, Cunha, Marco AV, Pinheiro, Daniel G, Maia, Rafaela M, ...

Establishment of the epithelial-specific transcriptome of normal and malignant human breast cells based on MPSS and array expression data

Grigoriadis, Anita, Mackay, Alan, Reis-Filho, Jorge S, Steele, Dawn, Iseli, Christian, Stevenson, Brian J, ...

Identification of unannotated exons of low abundance transcripts in Drosophila melanogaster and cloning of a new serine protease gene upregulated upon injury

Maia, Rafaela M, Valente, Valeria, Cunha, Marco AV, Sousa, Josane F, Araujo, Daniela D, Silva, Wilson A, ...