Biswas, Kabir Hassan, Shenoy, Avinash R, Dutta, Anindya, Visweswariah, Sandhya S
Guanylyl cyclases (GCs) are enzymes that generate cyclic GMP and regulate different physiologic and developmental processes in a number of organisms. GCs possess sequence similarity to class III...
A novel class of small RNAs: tRNA-derived RNA fragments (tRFs) (2009)
Lee, Yong Sun, Shibata, Yoshiyuki, Malhotra, Ankit, Dutta, Anindya
New types of small RNAs distinct from microRNAs (miRNAs) are progressively being discovered in various organisms. In order to discover such novel small RNAs, a library of 17- to 26-base-long RNAs was...
Torreira, Eva, Jha, Sudhakar, López-Blanco, José R., Arias-Palomo, Ernesto, Chacón, Pablo, Cañas, Cristina, ...
10 pages, 6 figures.-- PMID: 18940606 [PubMed].-- PMCID: PMC2577609 [Available on 2009/10/01].-- Supplementary information (Suppl. figures S1-S5, 5 pages) available at:...
Extraction of human DNA replication timing patterns from discrete microarray data (2008)
Dutta, Anindya, Karnani, Neerja, Malhotra, Ankit, Robins, Gabriel, Taylor, Christopher M.
Effective reproduction is essential for the survival and proliferation of any organism, from the birth of new offspring to the repro- duction of individual cells. Each portion of a cell’s DNA must...
Abbas, Tarek, Sivaprasad, Uma, Terai, Kenta, Amador, Virginia, Pagano, Michele, Dutta, Anindya
The DNA polymerase delta processivity factor Proliferating Cell Nuclear Antigen (PCNA) promotes the DNA damage-induced degradation of the replication initiation factor Cdt1 via the CRL4Cdt2 E3...
Extraction of human DNA replication timing patterns from discrete microarray data (2008)
Dutta, Anindya, Karnani, Neerja, Malhotra, Ankit, Robins, Gabriel, Taylor, Christopher M.
Effective reproduction is essential for the survival and proliferation of any organism, from the birth of new offspring to the repro- duction of individual cells. Each portion of a cell’s DNA must...
Zhu, Wenge, Ukomadu, Chinweike, Jha, Sudhakar, Senga, Takeshi, Dhar, Suman K., Wohlschlegel, James A., ...
The MCM2-7 helicase complex is loaded on DNA replication origins during the G1 phase of the cell cycle to license the origins for replication in S phase. How the initiator primase–polymerase...
Karnani, Neerja, Taylor, Christopher, Malhotra, Ankit, Dutta, Anindya
In eukaryotes, accurate control of replication time is required for the efficient completion of S phase and maintenance of genome stability. We present a high-resolution genome-tiling array-based...
The tumor suppressor microRNA let-7 represses the HMGA2 oncogene (2007)
HMGA2, a high-mobility group protein, is oncogenic in a variety of tumors, including benign mesenchymal tumors and lung cancers. Knockdown of Dicer in HeLa cells revealed that the HMGA2 gene is...
APC/C – the master controller of origin licensing? (2007)
Sivaprasad, Umasundari, Machida, Yuichi J, Dutta, Anindya
Abstract DNA replication must be tightly controlled to prevent initiation of a second round of replication until mitosis is complete. So far, components of the pre-replicative complex (Cdt1, Cdc6 and...
The APC/C inhibitor, Emi1, is essential for prevention of rereplication (2007)
Machida, Yuichi J., Dutta, Anindya
Emi1 (early mitotic inhibitor) inhibits APC/C (anaphase-promoting complex/cyclosome) activity during S and G2 phases, and is believed to be required for proper mitotic entry. We report that Emi1...
Takeda, David Y., Shibata, Yoshiyuki, Parvin, Jeffrey D., Dutta, Anindya
Origins of replication are expected to recruit initiation proteins like origin recognition complex (ORC) and Cdc6 in eukaryotes and provide a platform for unwinding DNA. Here we test whether...
Action of the p53 Effector, p21, on its Targets: Cyclin-cdk and PCNA (2003)
While studying the action of p21 on cyclin-cdks a 12 amino acid peptide, the Cy motif, was shown to be sufficient for inhibiting the protein kinase. The Cy motif was subsequently found on several...
On energy based seismic analysis and design of highway bridges /--by Anindya Dutta. (1999)
Photocopy of typescript.
Interaction of the Tumor Suppressor p53 with Replication Protein A. (1998)
The DNA replication factor RPA physically associates with the tumor suppressor protein p53, an interaction that could be important for the function of both these proteins in normal and cancer cells....
Interaction of the Tumor Suppressor p53 With Replication Protein A. (1998)
Cdc25A, a phosphatase essential for Gl-S transition, associates with, dephosphorylates and activates the cell-cycle regulator cyclin-dependent kinase (cdk). p21 is a cdk inhibitor induced by a tumor...
Cellular Proteins Interacting with the Tumor Suppressor Protein p53. (1998)
Tumor suppressor protein p53 interacts directly with the DNA replication factor RPA and inhibits its ability to bind single-strand DNA. We defined the domain of p53 that bound to RPA and constructed...
Interaction of the Tumor Suppressor p53 With Replication Protein A. (1998)
In order to understand how the tumor suppressor p53 blocks the cell-cycle at the G1-S transition we studied how p21, a transcription target of p53, interacts with and inhibits cyclin-dependent...
Action of the p53 Effector, p2l, on its Targets: Cyclin cdk and PCNA (1998)
Our aim is to dissect the interactions of p21 (a downstream effector of the tumor suppressor protein p53) with its two target, cyclin-cdks and PCNA. In year 1 we have established conditions for...
Action of the p53 Effector, p21, on its Targets: Cyclin-CDK & PCNA (1998)
Our aim is to dissect the interactions of p21 (a downstream effector of the tumor suppressor protein p53) with its targets, cyclin-cdks and PCNA. In year 2 we have focused on the cyclin-cdks. We have...
Action of the p53 Effector, p21, on its Targets: Cyclin-cdk and PCNA (1998)
Cy or RXL motifs have been previously shown to be cyclin binding motifs found in a wide range of cyclin-cdk interacting proteins. For both cyclin A/cdk2 and cyclin E/cdk2, the presence of a Cy motif...
Mapping Critical DNA Sequence Elements Required for Amplification of erbB2 in Breast Cancer (1998)
Machida, Yuichi, Dutta, Anindya
Recent studies show that 80-90% of breast carcinomas are associated with gene amplification. Each amplicon most likely has an origin of replication. In Drosophila, DmORC (Origin Recognition Complex)...
Wohlschlegel, James, Dutta, Anindya
Upregulation of cyclin/cdk activity and the resulting deregulation of the cell cycle is a common feature in the malignant transformation of breast epithelial cells and thus makes cyclin/cdks an...
Mapping Critical DNA Sequence Elements Required for Amplification of erbB2 in Breast Cancer (1998)
Machida, Yuichi, Dutta, Anindya
To study the implications of replication initiator proteins to gene amplification, we characterized two proteins required for DNA replication (ORC and Mcm10). In an attempt to purify recombinant...
The Origin Recognition Complex is thought to recognize Origins of Replication and recruit replication initiation factors in mammalian cells. The loading of this complex on DNA origins is required for...
The Origin Recognition Complex is thought to recognize Origins of Replication and recruit replication initiation factors in mammalian cells. The loading of this complex on DNA origins is required for...
The Origin Recognition Complex (ORC) is thought to function in the loading of replication proteins on the chromatin. In lower organisms, chromatin loading of ORC is required for replication, and...
Interaction of the Tumor Suppressor p53 with Replication Protein A. (1997)
The DNA replication factor RPA physically associates with the tumor suppressor protein p53, an interaction that could be important for the function of both these proteins in normal and cancer cells....
Fatigue analysis of non-ductile concrete columns /--by Anindya Dutta. (1995)
Photocopy of typescript.
Thesis (Ph. D.)--Rockefeller University.
Human DNA replication initiation factors, ORC and MCM, associate with oriP of Epstein–Barr virus
Chaudhuri, Biswendu, Xu, Hongzhan, Todorov, Ivan, Dutta, Anindya, Yates, John L.
The 165-kb chromosome of Epstein–Barr virus (EBV) is replicated by cellular enzymes only once per cell cycle in human cells that are latently infected. Here, we report that the human origin...
Requirement of CDC45 for Postimplantation Mouse Development
Yoshida, Kenichi, Kuo, Frank, George, Elizabeth L., Sharpe, Arlene H., Dutta, Anindya
CDC45 is required for the initiation of DNA replication in Saccharomyces cerevisiae and functions as a DNA polymerase α loading factor in Xenopus, but its role in mammalian DNA replication is...
Wohlschlegel, James A., Dwyer, Brian T., Takeda, David Y., Dutta, Anindya
Inhibitors, activators, and substrates of cyclin-dependent kinases (cdks) utilize a cyclin-binding sequence, known as a Cy or RXL motif, to bind directly to the cyclin subunit. Alanine scanning...
Saha, Partha, Chen, Junjie, Thome, Kelly C., Lawlis, Stephanie J., Hou, Zhi-hui, Hendricks, Marvin, ...
In a two-hybrid screen for proteins that interact with human PCNA, we identified and cloned a human protein (hCdc18) homologous to yeast CDC6/Cdc18 and human Orc1. Unlike yeast, in which the rapid...
Zhu, Wenge, Chen, Yuefeng, Dutta, Anindya
Genomic DNA replication is tightly controlled to ensure that DNA replication occurs once per cell cycle; loss of this control leads to genomic instability. Geminin, a DNA replication inhibitor, plays...
Temporal profile of replication of human chromosomes
Jeon, Yesu, Bekiranov, Stefan, Karnani, Neerja, Kapranov, Philipp, Ghosh, Srinka, MacAlpine, David, ...
Chromosomes in human cancer cells are expected to initiate replication from predictably localized origins, firing reproducibly at discrete times in S phase. Replication products obtained from HeLa...
Takeda, David Y., Shibata, Yoshiyuki, Parvin, Jeffrey D., Dutta, Anindya
Origins of replication are expected to recruit initiation proteins like origin recognition complex (ORC) and Cdc6 in eukaryotes and provide a platform for unwinding DNA. Here we test whether...
The timely assembly of prereplicative complexes at replication origins is tightly controlled to ensure that genomic DNA is replicated once per cell cycle. The loss of geminin, a DNA replication...
Human DNA replication initiation factors, ORC and MCM, associate with oriP of Epstein–Barr virus
Chaudhuri, Biswendu, Xu, Hongzhan, Todorov, Ivan, Dutta, Anindya, Yates, John L.
The 165-kb chromosome of Epstein–Barr virus (EBV) is replicated by cellular enzymes only once per cell cycle in human cells that are latently infected. Here, we report that the human origin...
Requirement of CDC45 for Postimplantation Mouse Development
Yoshida, Kenichi, Kuo, Frank, George, Elizabeth L., Sharpe, Arlene H., Dutta, Anindya
CDC45 is required for the initiation of DNA replication in Saccharomyces cerevisiae and functions as a DNA polymerase α loading factor in Xenopus, but its role in mammalian DNA replication is...
Wohlschlegel, James A., Dwyer, Brian T., Takeda, David Y., Dutta, Anindya
Inhibitors, activators, and substrates of cyclin-dependent kinases (cdks) utilize a cyclin-binding sequence, known as a Cy or RXL motif, to bind directly to the cyclin subunit. Alanine scanning...
Saha, Partha, Chen, Junjie, Thome, Kelly C., Lawlis, Stephanie J., Hou, Zhi-hui, Hendricks, Marvin, ...
In a two-hybrid screen for proteins that interact with human PCNA, we identified and cloned a human protein (hCdc18) homologous to yeast CDC6/Cdc18 and human Orc1. Unlike yeast, in which the rapid...
Zhu, Wenge, Chen, Yuefeng, Dutta, Anindya
Genomic DNA replication is tightly controlled to ensure that DNA replication occurs once per cell cycle; loss of this control leads to genomic instability. Geminin, a DNA replication inhibitor, plays...
Temporal profile of replication of human chromosomes
Jeon, Yesu, Bekiranov, Stefan, Karnani, Neerja, Kapranov, Philipp, Ghosh, Srinka, MacAlpine, David, ...
Chromosomes in human cancer cells are expected to initiate replication from predictably localized origins, firing reproducibly at discrete times in S phase. Replication products obtained from HeLa...
Takeda, David Y., Shibata, Yoshiyuki, Parvin, Jeffrey D., Dutta, Anindya
Origins of replication are expected to recruit initiation proteins like origin recognition complex (ORC) and Cdc6 in eukaryotes and provide a platform for unwinding DNA. Here we test whether...
The timely assembly of prereplicative complexes at replication origins is tightly controlled to ensure that genomic DNA is replicated once per cell cycle. The loss of geminin, a DNA replication...
Machida, Yuichi J., Chen, Yuefeng, Machida, Yuka, Malhotra, Ankit, Sarkar, Sukumar, Dutta, Anindya
Differences in the genetic and epigenetic make up of cell lines have been very useful for dissecting the roles of specific genes in the biology of a cell. Targeted comparative RNAi (TARCOR) analysis...
APC/C – the master controller of origin licensing?
Sivaprasad, Umasundari, Machida, Yuichi J, Dutta, Anindya
DNA replication must be tightly controlled to prevent initiation of a second round of replication until mitosis is complete. So far, components of the pre-replicative complex (Cdt1, Cdc6 and geminin)...
Expression of Geminin as a Marker of Cell Proliferation in Normal Tissues and Malignancies
Wohlschlegel, James A., Kutok, Jeffery L., Weng, Andrew P., Dutta, Anindya
Geminin interacts with a DNA replication initiation factor, Cdt1p, to suppress initiation of DNA replication in a Xenopus egg extract based cell-free system, leading to the expectation that the...
The APC/C inhibitor, Emi1, is essential for prevention of rereplication
Machida, Yuichi J., Dutta, Anindya
Emi1 (early mitotic inhibitor) inhibits APC/C (anaphase-promoting complex/cyclosome) activity during S and G2 phases, and is believed to be required for proper mitotic entry. We report that Emi1...
The tumor suppressor microRNA let-7 represses the HMGA2 oncogene
HMGA2, a high-mobility group protein, is oncogenic in a variety of tumors, including benign mesenchymal tumors and lung cancers. Knockdown of Dicer in HeLa cells revealed that the HMGA2 gene is...
Muscle-specific microRNA miR-206 promotes muscle differentiation
Kim, Hak Kyun, Lee, Yong Sun, Sivaprasad, Umasundari, Malhotra, Ankit, Dutta, Anindya
Three muscle-specific microRNAs, miR-206, -1, and -133, are induced during differentiation of C2C12 myoblasts in vitro. Transfection of miR-206 promotes differentiation despite the presence of serum,...
Karnani, Neerja, Taylor, Christopher, Malhotra, Ankit, Dutta, Anindya
In eukaryotes, accurate control of replication time is required for the efficient completion of S phase and maintenance of genome stability. We present a high-resolution genome-tiling array-based...
Mcm10 and And-1/CTF4 recruit DNA polymerase α to chromatin for initiation of DNA replication
Zhu, Wenge, Ukomadu, Chinweike, Jha, Sudhakar, Senga, Takeshi, Dhar, Suman K., Wohlschlegel, James A., ...
The MCM2-7 helicase complex is loaded on DNA replication origins during the G1 phase of the cell cycle to license the origins for replication in S phase. How the initiator primase–polymerase...
Alpi, Arno, Langevin, Frederic, Mosedale, Georgina, Machida, Yuichi J., Dutta, Anindya, Patel, Ketan J.
The Fanconi anemia (FA) nuclear core complex and the E2 ubiquitin-conjugating enzyme UBE2T are required for the S phase and DNA damage-restricted monoubiquitination of FANCD2. This constitutes a key...
Jha, Sudhakar, Shibata, Etsuko, Dutta, Anindya
The role of chromatin-remodeling factors in transcription is well established, but the link between chromatin-remodeling complexes and DNA repair remains unexplored. Human Rvb1 and Rvb2 are highly...
Abbas, Tarek, Sivaprasad, Uma, Terai, Kenta, Amador, Virginia, Pagano, Michele, Dutta, Anindya
The DNA polymerase delta processivity factor Proliferating Cell Nuclear Antigen (PCNA) promotes the DNA damage-induced degradation of the replication initiation factor Cdt1 via the CRL4Cdt2 E3...
Shibata, Yoshiyuki, Malhotra, Ankit, Bekiranov, Stefan, Dutta, Anindya
Paired end mapping of chromosomal fragments has been used in human cells to identify numerous structural variations in chromosomes of individuals and of cancer cell lines; however, the molecular,...