P. B. Viola, Er Kiani, Patricia T. Bozza, Anjana Rao
Transcription factors of the NFAT (nuclear factor of activated T cells) family regulate the expression of many genes encoding immunoregulatory cytokines and cell surface proteins during the immune...
Gene regulation and signal transduction in the immune system (2008)
Horng, Tiffany, Oberdoerffer, Shalini, Rao, Anjana
A report of the meeting 'Gene Expression and Signaling in the Immune System', Cold Spring Harbor, USA, 22-26 April 2008.
Hair loss and defective T- and B-cell function in mice lacking ORAI1 (2008)
Gwack, Yousang, Srikanth, Sonal, Oh-Hora, Masatsugu, Hogan, Patrick G., Lamperti, Edward D., Yamashita, Megumi, ...
ORAI1 is a pore subunit of the store-operated Ca(2+) release-activated Ca(2+) (CRAC) channel. To examine the physiological consequences of ORAI1 deficiency, we generated mice with targeted disruption...
Mouse Eri1 interacts with the ribosome and catalyzes 5.8S rRNA processing (2008)
Ansel, K. Mark, Pastor, William A., Rath, Nicola, Lapan, Ariya D., Glasmacher, Elke, Wolf, Christine, ...
Eri1 is a 3'-to-5' exoribonuclease conserved from fission yeast to humans. Here we show that Eri1 associates with ribosomes and ribosomal RNA (rRNA). Ribosomes from Eri1-deficient mice contain 5.8S...
Immunological applications of genomics (2006)
Monticelli, Silvia, Sharma, Sonia, Rao, Anjana
A report of the Cold Spring Harbor Laboratory meeting 'Gene Expression and Signaling in the Immune System', Cold Spring Harbor, New York, USA, 26-30 April 2006.
MicroRNA profiling of the murine hematopoietic system (2005)
Monticelli, Silvia, Ansel, K Mark, Xiao, Changchun, Socci, Nicholas D, Krichevsky, Anna M, Thai, To-Ha, ...
Abstract Background MicroRNAs (miRNAs) are a class of recently discovered noncoding RNA genes that post-transcriptionally regulate gene expression. It is becoming clear that miRNAs play an important...
Chromatin-based regulation of cytokine transcription in Th2 cells and mast cells (2005)
Monticelli, Silvia, Lee, Dong U., Nardone, Julie, Bolton, Diana L., Rao, Anjana
Th2 cells and mast cells are major sources of IL4, IL5 and IL13, cytokines that mediate immunity against parasites and are also central players in the pathophysiology of asthma, allergy and atopic...
Chromatin-based regulation of cytokine transcription in Th2 cells and mast cells (2005)
Monticelli, Silvia, Lee, Dong U., Nardone, Julie, Bolton, Diana L., Rao, Anjana
Th2 cells and mast cells are major sources of IL4, IL5 and IL13, cytokines that mediate immunity against parasites and are also central players in the pathophysiology of asthma, allergy and atopic...
The role of NFAT transcription factors in integrin-mediated carcinoma invasion. (2002)
Jauliac, Sebastien, Lopez-Rodriguez, Cristina, Shaw, Leslie, Brown, Lawrence, Rao, Anjana, Toker, Alex
Integrins, receptors for extracellular matrix ligands, are critical regulators of the invasive phenotype. Specifically, the alpha(6)beta(4) integrin has been linked with epithelial cell motility,...
Molecular aspects of T-cell differentiation (2000)
Differentiated T helper 1(Th1) and T helper 2(Th2) T-cells show striking differences in their patterns of cytokine expression. This process is initiated by stimulation with antigen and the cytokines...
Role Of The Cyclosporin-sensitive Transcription Factor NFAT1 In The Allergic Response (1997)
Proteins belonging to the NFAT (nuclear factor of activated T cells) family of transcription factors are expressed in most immune cell types, and play a central role in the transcription of cytokine...
Role of the cyclosporin-sensitive transcription factor NFAT1 in the allergic response (1997)
Proteins belonging to the NFAT (nuclear factor of activated T cells) family of transcription factors are expressed in most immune cell types, and play a central role in the transcription of cytokine...
Martínez-Martínez, Sara, Gómez Del Arco, Pablo, Armesilla, Angel Luis, Aramburu, Jose, Luo, Chun, Rao, Anjana, ...
Dithiocarbamates (DTCs) have recently been reported as powerful inhibitors of NF-kappaB activation in a number of cell types. Given the role of this transcription factor in the regulation of gene...
Submitted to: Committee on Higher Degrees in Biophysics.
NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun
López-Rodríguez, Cristina, Aramburu, José, Rakeman, Andrew S., Rao, Anjana
NFAT transcription factors are related to NF-κB/Rel proteins and form cooperative complexes with Fos and Jun on DNA. We have identified an NFAT-related protein, NFAT5, which differs from the...
Human immunodeficiency virus type 1 (HIV-1) gene expression is regulated by interactions between both viral and host factors. These interactions are also responsible for changes in the expression of...
Macián, Fernando, García-Rodríguez, Carmen, Rao, Anjana
Cooperation between nuclear factor of activated T cells (NFAT) and AP-1 (Fos–Jun) proteins on composite NFAT–AP-1 DNA elements constitutes a powerful mechanism for signal integration of the...
Loss of NFAT5 results in renal atrophy and lack of tonicity-responsive gene expression
López-Rodríguez, Cristina, Antos, Christopher L., Shelton, John M., Richardson, James A., Lin, Fangming, Novobrantseva, Tatiana I., ...
The transcription factor NFAT5/TonEBP, a member of the NFAT/Rel family of transcription factors, has been implicated in diverse cellular responses, including the response to osmotic stress,...
Degradation of Bcl10 Induced by T-Cell Activation Negatively Regulates NF-κB Signaling
Scharschmidt, Erika, Wegener, Elmar, Heissmeyer, Vigo, Rao, Anjana, Krappmann, Daniel
Bcl10 is a critical regulator of NF-κB activity in T and B cells, coupling antigen receptor signaling to NF-κB activation via protein kinase C (PKC). Here we show that PKC or T-cell receptor...
A Conserved Docking Motif for CK1 Binding Controls the Nuclear Localization of NFAT1
Okamura, Heidi, Garcia-Rodriguez, Carmen, Martinson, Holly, Qin, Jun, Virshup, David M., Rao, Anjana
In resting cells, the NFAT1 transcription factor is kept inactive in the cytoplasm by phosphorylation on multiple serine residues. These phosphorylated residues are primarily contained within two...
Roehrl, Michael H. A., Kang, Sunghyun, Aramburu, José, Wagner, Gerhard, Rao, Anjana, Hogan, Patrick G.
Transient or reversible protein–protein interactions are commonly used to ensure efficient targeting of signaling enzymes to their cellular substrates. These interactions include direct binding to...
Ikeda, Fumiyo, Nishimura, Riko, Matsubara, Takuma, Tanaka, Sakae, Inoue, Jun-ichiro, Reddy, Sakamuri V., ...
Receptor activator of NF-κB ligand (RANKL) plays an essential role in osteoclast formation and bone resorption. Although genetic and biochemical studies indicate that RANKL regulates osteoclast...
The linked IL-4 and IL-13 cytokine genes, which are activated and silenced in T helper (Th) 2 and Th1 cells, respectively, are flanked by the equivalently expressed RAD50 and KIF3A genes. A scan of...
Oberdoerffer, Philipp, Kanellopoulou, Chryssa, Heissmeyer, Vigo, Paeper, Corinna, Borowski, Christine, Aifantis, Iannis, ...
RNA interference (RNAi) is a naturally occurring posttranscriptional gene-silencing mechanism that has been adapted as a genetic tool for loss-of-function studies of a variety of organisms. It is...
Esensten, Jonathan H., Tsytsykova, Alla V., Lopez-Rodriguez, Cristina, Ligeiro, Filipa A., Rao, Anjana, Goldfeld, Anne E.
Tumor necrosis factor (TNF) is a pro-inflammatory cytokine that plays an important role in a variety of infectious and autoimmune disorders. Its transcription is regulated in a stimulus- and...
MicroRNA profiling of the murine hematopoietic system
Monticelli, Silvia, Ansel, K Mark, Xiao, Changchun, Socci, Nicholas D, Krichevsky, Anna M, Thai, To-Ha, ...
The first report of systematic miRNA profiling in cells of the hematopoietic system suggests that, in addition to regulating commitment to particular cellular lineages, miRNAs might have a general...
Kumar, Lalit, Feske, Stefan, Rao, Anjana, Geha, Raif S.
The adapter SLP-76 is essential for T cell development and function. SLP-76 binds to the src homology 3 domain of Lck in vitro. This interaction depends on amino acids 185–194 of SLP-76. To examine...
NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun
López-Rodríguez, Cristina, Aramburu, José, Rakeman, Andrew S., Rao, Anjana
NFAT transcription factors are related to NF-κB/Rel proteins and form cooperative complexes with Fos and Jun on DNA. We have identified an NFAT-related protein, NFAT5, which differs from the...
Human immunodeficiency virus type 1 (HIV-1) gene expression is regulated by interactions between both viral and host factors. These interactions are also responsible for changes in the expression of...
Macián, Fernando, García-Rodríguez, Carmen, Rao, Anjana
Cooperation between nuclear factor of activated T cells (NFAT) and AP-1 (Fos–Jun) proteins on composite NFAT–AP-1 DNA elements constitutes a powerful mechanism for signal integration of the...
Loss of NFAT5 results in renal atrophy and lack of tonicity-responsive gene expression
López-Rodríguez, Cristina, Antos, Christopher L., Shelton, John M., Richardson, James A., Lin, Fangming, Novobrantseva, Tatiana I., ...
The transcription factor NFAT5/TonEBP, a member of the NFAT/Rel family of transcription factors, has been implicated in diverse cellular responses, including the response to osmotic stress,...
Degradation of Bcl10 Induced by T-Cell Activation Negatively Regulates NF-κB Signaling
Scharschmidt, Erika, Wegener, Elmar, Heissmeyer, Vigo, Rao, Anjana, Krappmann, Daniel
Bcl10 is a critical regulator of NF-κB activity in T and B cells, coupling antigen receptor signaling to NF-κB activation via protein kinase C (PKC). Here we show that PKC or T-cell receptor...
A Conserved Docking Motif for CK1 Binding Controls the Nuclear Localization of NFAT1
Okamura, Heidi, Garcia-Rodriguez, Carmen, Martinson, Holly, Qin, Jun, Virshup, David M., Rao, Anjana
In resting cells, the NFAT1 transcription factor is kept inactive in the cytoplasm by phosphorylation on multiple serine residues. These phosphorylated residues are primarily contained within two...
Roehrl, Michael H. A., Kang, Sunghyun, Aramburu, José, Wagner, Gerhard, Rao, Anjana, Hogan, Patrick G.
Transient or reversible protein–protein interactions are commonly used to ensure efficient targeting of signaling enzymes to their cellular substrates. These interactions include direct binding to...
Ikeda, Fumiyo, Nishimura, Riko, Matsubara, Takuma, Tanaka, Sakae, Inoue, Jun-ichiro, Reddy, Sakamuri V., ...
Receptor activator of NF-κB ligand (RANKL) plays an essential role in osteoclast formation and bone resorption. Although genetic and biochemical studies indicate that RANKL regulates osteoclast...
The linked IL-4 and IL-13 cytokine genes, which are activated and silenced in T helper (Th) 2 and Th1 cells, respectively, are flanked by the equivalently expressed RAD50 and KIF3A genes. A scan of...
Oberdoerffer, Philipp, Kanellopoulou, Chryssa, Heissmeyer, Vigo, Paeper, Corinna, Borowski, Christine, Aifantis, Iannis, ...
RNA interference (RNAi) is a naturally occurring posttranscriptional gene-silencing mechanism that has been adapted as a genetic tool for loss-of-function studies of a variety of organisms. It is...
Esensten, Jonathan H., Tsytsykova, Alla V., Lopez-Rodriguez, Cristina, Ligeiro, Filipa A., Rao, Anjana, Goldfeld, Anne E.
Tumor necrosis factor (TNF) is a pro-inflammatory cytokine that plays an important role in a variety of infectious and autoimmune disorders. Its transcription is regulated in a stimulus- and...
MicroRNA profiling of the murine hematopoietic system
Monticelli, Silvia, Ansel, K Mark, Xiao, Changchun, Socci, Nicholas D, Krichevsky, Anna M, Thai, To-Ha, ...
The first report of systematic miRNA profiling in cells of the hematopoietic system suggests that, in addition to regulating commitment to particular cellular lineages, miRNAs might have a general...
Kumar, Lalit, Feske, Stefan, Rao, Anjana, Geha, Raif S.
The adapter SLP-76 is essential for T cell development and function. SLP-76 binds to the src homology 3 domain of Lck in vitro. This interaction depends on amino acids 185–194 of SLP-76. To examine...
Immunological applications of genomics
Monticelli, Silvia, Sharma, Sonia, Rao, Anjana
A report of the Cold Spring Harbor Laboratory meeting 'Gene Expression and Signaling in the Immune System', Cold Spring Harbor, New York, USA, 26-30 April 2006.
Feske, Stefan, Prakriya, Murali, Rao, Anjana, Lewis, Richard S.
Engagement of the TCR triggers sustained Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels, which helps drive gene expression underlying the T cell response to pathogens. The identity...
Aberrant T cell differentiation in the absence of Dicer
Muljo, Stefan A., Ansel, K. Mark, Kanellopoulou, Chryssa, Livingston, David M., Rao, Anjana, Rajewsky, Klaus
Dicer is an RNaseIII-like enzyme that is required for generating short interfering RNAs and microRNAs. The latter have been implicated in regulating cell fate determination in invertebrates and...
García-Rodríguez, Carmen, Rao, Anjana
p300 and cAMP response element–binding protein (CREB)–binding protein (CBP) are members of a family of coactivators involved in the regulation of transcription and chromatin. We show that...
Barr, Valarie A., Bernot, Kelsie M., Srikanth, Sonal, Gwack, Yousang, Balagopalan, Lakshmi, Regan, Carole K., ...
The proteins STIM1 and Orai1 are the long sought components of the store-operated channels required in T-cell activation. However, little is known about the interaction of these proteins in T-cells...
Gene regulation and signal transduction in the immune system
Horng, Tiffany, Oberdoerffer, Shalini, Rao, Anjana
A report of the meeting 'Gene Expression and Signaling in the Immune System', Cold Spring Harbor, USA, 22-26 April 2008.
Hair Loss and Defective T- and B-Cell Function in Mice Lacking ORAI1▿ †
Gwack, Yousang, Srikanth, Sonal, Oh-hora, Masatsugu, Hogan, Patrick G., Lamperti, Edward D., Yamashita, Megumi, ...
ORAI1 is a pore subunit of the store-operated Ca2+ release-activated Ca2+ (CRAC) channel. To examine the physiological consequences of ORAI1 deficiency, we generated mice with targeted disruption of...
Runx3 and T-box proteins cooperate to establish the transcriptional program of effector CTLs
Cruz-Guilloty, Fernando, Pipkin, Matthew E., Djuretic, Ivana M., Levanon, Ditsa, Lotem, Joseph, Lichtenheld, Mathias G., ...
Activation of naive CD8+ T cells with antigen induces their differentiation into effector cytolytic T lymphocytes (CTLs). CTLs lyse infected or aberrant target cells by exocytosis of lytic granules...
Transcriptional complexes formed by NFAT dimers regulate the induction of T cell tolerance
Soto-Nieves, Noemi, Puga, Irene, Abe, Brian T., Bandyopadhyay, Sanmay, Baine, Ian, Rao, Anjana, ...
In T cells, anergy can be induced after T cell receptor engagement in the absence of costimulation. Under these conditions, the expression of a specific set of anergy-associated genes is activated....
Requirement for balanced Ca/NFAT signaling in hematopoietic and embryonic development
Müller, Martin R., Sasaki, Yoshiteru, Stevanovic, Irena, Lamperti, Edward D., Ghosh, Srimoyee, Sharma, Sonia, ...
NFAT transcription factors are highly phosphorylated proteins residing in the cytoplasm of resting cells. Upon dephosphorylation by the phosphatase calcineurin, NFAT proteins translocate to the...