Ann L. Beyer

Mrd1p binds to pre-rRNA early during transcription independent of U3 snoRNA and is required for compaction of the pre-rRNA into small subunit processomes (2008)

Segerstolpe, Åsa, Lundkvist, Pär, Osheim, Yvonne N., Beyer, Ann L., Wieslander, Lars

In Saccharomyces cerevisiae, synthesis of the small ribosomal subunit requires assembly of the 35S pre-rRNA into a 90S preribosomal complex. SnoRNAs, including U3 snoRNA, and many trans-acting...

TOR regulates the subcellular distribution of DIM2, a KH domain protein required for cotranscriptional ribosome assembly and pre-40S ribosome export (2008)

Vanrobays, Emmanuel, Leplus, Alexis, Osheim, Yvonne N., Beyer, Ann L., Wacheul, Ludivine, Lafontaine, Denis L.J.

Eukaryotic ribosome synthesis is a highly dynamic process that involves the transient association of scores of trans-acting factors to nascent pre-ribosomes. Many ribosome synthesis factors are...

RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components (2004)

Gallagher, Jennifer E.G., Dunbar, David A., Granneman, Sander, Mitchell, Brianna M., Osheim, Yvonne, Beyer, Ann L., ...

Sequential events in macromolecular biosynthesis are often elegantly coordinated. The small ribosomal subunit (SSU) processome is a large ribonucleoprotein (RNP) required for processing of precursors...

The Drosophila Hrb87F gene encodes a new member of the A and B hnRNP protein group (1991)

R.Haynes, Susan, Johnson, Diana, Raychaudhuri, Gopa, Beyer, Ann L.

Nascent premessenger RNA transcripts are packaged into heterogeneous nuclear ribonucleoprotein (hnRNP) complexes containing specific nuclear proteins, the hnRNP proteins. The A and B group proteins...

RPD3 is required for the inactivation of yeast ribosomal DNA genes in stationary phase

Sandmeier, Joseph J., French, Sarah, Osheim, Yvonne, Cheung, Wang L., Gallo, Christopher M., Beyer, Ann L., ...

rRNA transcription in Saccharomyces cerevisiae is performed by RNA polymerase I and regulated by changes in growth conditions. During log phase, ∼50% of the ribosomal DNA (rDNA) genes in each cell...

In Exponentially Growing Saccharomyces cerevisiae Cells, rRNA Synthesis Is Determined by the Summed RNA Polymerase I Loading Rate Rather than by the Number of Active Genes

French, Sarah L., Osheim, Yvonne N., Cioci, Francesco, Nomura, Masayasu, Beyer, Ann L.

Genes encoding rRNA are multicopy and thus could be regulated by changing the number of active genes or by changing the transcription rate per gene. We tested the hypothesis that the number of open...

Tor Pathway Regulates Rrn3p-dependent Recruitment of Yeast RNA Polymerase I to the Promoter but Does Not Participate in Alteration of the Number of Active Genes

Claypool, Jonathan A., French, Sarah L., Johzuka, Katsuki, Eliason, Kristilyn, Vu, Loan, Dodd, Jonathan A., ...

Yeast cells entering into stationary phase decrease rRNA synthesis rate by decreasing both the number of active genes and the transcription rate of individual active genes. Using chromatin...

Transcriptional termination by RNA polymerase I requires the small subunit Rpa12p

Prescott, Elizabeth M., Osheim, Yvonne N., Jones, Hannah S., Alen, Claudia M., Roan, Judith G., Reeder, Ronald H., ...

We identify Rpa12p of RNA polymerase I (Pol I) as a termination factor. Combined analyses using transcription run-on, electron microscopy-visualized chromatin spreading and RT-PCR have been applied...

Adenovirus Protein VII Condenses DNA, Represses Transcription, and Associates with Transcriptional Activator E1A

Johnson, Jeffrey S., Osheim, Yvonne N., Xue, Yuming, Emanuel, Margaux R., Lewis, Peter W., Bankovich, Alex, ...

Adenovirus protein VII is the major protein component of the viral nucleoprotein core. It is highly basic, and an estimated 1070 copies associate with each viral genome, forming a tightly condensed...

RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components

Gallagher, Jennifer E.G., Dunbar, David A., Granneman, Sander, Mitchell, Brianna M., Osheim, Yvonne, Beyer, Ann L., ...

Sequential events in macromolecular biosynthesis are often elegantly coordinated. The small ribosomal subunit (SSU) processome is a large ribonucleoprotein (RNP) required for processing of precursors...

Esf2p, a U3-Associated Factor Required for Small-Subunit Processome Assembly and Compaction

Hoang, Tran, Peng, Wen-Tao, Vanrobays, Emmanuel, Krogan, Nevan, Hiley, Shawna, Beyer, Ann L., ...

Esf2p is the Saccharomyces cerevisiae homolog of mouse ABT1, a protein previously identified as a putative partner of the TATA-element binding protein. However, large-scale studies have indicated...

Histones are required for transcription of yeast rRNA genes by RNA polymerase I

Tongaonkar, Prasad, French, Sarah L., Oakes, Melanie L., Vu, Loan, Schneider, David A., Beyer, Ann L., ...

Nucleosomes and their histone components have generally been recognized to act negatively on transcription. However, purified upstream activating factor (UAF), a transcription initiation factor...

Role of Histone Deacetylase Rpd3 in Regulating rRNA Gene Transcription and Nucleolar Structure in Yeast

Oakes, Melanie L., Siddiqi, Imran, French, Sarah L., Vu, Loan, Sato, Manabu, Aris, John P., ...

The 35S rRNA genes at the RDN1 locus in Saccharomyces cerevisiae can be transcribed by RNA polymerase (Pol) II in addition to Pol I, but Pol II transcription is usually silenced. The deletion of RRN9...

The PINc domain protein Utp24, a putative nuclease, is required for the early cleavage steps in 18S rRNA maturation

Bleichert, Franziska, Granneman, Sander, Osheim, Yvonne N., Beyer, Ann L., Baserga, Susan J.

Ribosome biogenesis is a complex process that requires >150 transacting factors, many of which form macromolecular assemblies as big and complex as the ribosome itself. One of those complexes, the...

RPD3 is required for the inactivation of yeast ribosomal DNA genes in stationary phase

Sandmeier, Joseph J., French, Sarah, Osheim, Yvonne, Cheung, Wang L., Gallo, Christopher M., Beyer, Ann L., ...

rRNA transcription in Saccharomyces cerevisiae is performed by RNA polymerase I and regulated by changes in growth conditions. During log phase, ∼50% of the ribosomal DNA (rDNA) genes in each cell...

In Exponentially Growing Saccharomyces cerevisiae Cells, rRNA Synthesis Is Determined by the Summed RNA Polymerase I Loading Rate Rather than by the Number of Active Genes

French, Sarah L., Osheim, Yvonne N., Cioci, Francesco, Nomura, Masayasu, Beyer, Ann L.

Genes encoding rRNA are multicopy and thus could be regulated by changing the number of active genes or by changing the transcription rate per gene. We tested the hypothesis that the number of open...

Tor Pathway Regulates Rrn3p-dependent Recruitment of Yeast RNA Polymerase I to the Promoter but Does Not Participate in Alteration of the Number of Active Genes

Claypool, Jonathan A., French, Sarah L., Johzuka, Katsuki, Eliason, Kristilyn, Vu, Loan, Dodd, Jonathan A., ...

Yeast cells entering into stationary phase decrease rRNA synthesis rate by decreasing both the number of active genes and the transcription rate of individual active genes. Using chromatin...

Transcriptional termination by RNA polymerase I requires the small subunit Rpa12p

Prescott, Elizabeth M., Osheim, Yvonne N., Jones, Hannah S., Alen, Claudia M., Roan, Judith G., Reeder, Ronald H., ...

We identify Rpa12p of RNA polymerase I (Pol I) as a termination factor. Combined analyses using transcription run-on, electron microscopy-visualized chromatin spreading and RT-PCR have been applied...

Adenovirus Protein VII Condenses DNA, Represses Transcription, and Associates with Transcriptional Activator E1A

Johnson, Jeffrey S., Osheim, Yvonne N., Xue, Yuming, Emanuel, Margaux R., Lewis, Peter W., Bankovich, Alex, ...

Adenovirus protein VII is the major protein component of the viral nucleoprotein core. It is highly basic, and an estimated 1070 copies associate with each viral genome, forming a tightly condensed...

RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components

Gallagher, Jennifer E.G., Dunbar, David A., Granneman, Sander, Mitchell, Brianna M., Osheim, Yvonne, Beyer, Ann L., ...

Sequential events in macromolecular biosynthesis are often elegantly coordinated. The small ribosomal subunit (SSU) processome is a large ribonucleoprotein (RNP) required for processing of precursors...

Esf2p, a U3-Associated Factor Required for Small-Subunit Processome Assembly and Compaction

Hoang, Tran, Peng, Wen-Tao, Vanrobays, Emmanuel, Krogan, Nevan, Hiley, Shawna, Beyer, Ann L., ...

Esf2p is the Saccharomyces cerevisiae homolog of mouse ABT1, a protein previously identified as a putative partner of the TATA-element binding protein. However, large-scale studies have indicated...

Histones are required for transcription of yeast rRNA genes by RNA polymerase I

Tongaonkar, Prasad, French, Sarah L., Oakes, Melanie L., Vu, Loan, Schneider, David A., Beyer, Ann L., ...

Nucleosomes and their histone components have generally been recognized to act negatively on transcription. However, purified upstream activating factor (UAF), a transcription initiation factor...

Role of Histone Deacetylase Rpd3 in Regulating rRNA Gene Transcription and Nucleolar Structure in Yeast

Oakes, Melanie L., Siddiqi, Imran, French, Sarah L., Vu, Loan, Sato, Manabu, Aris, John P., ...

The 35S rRNA genes at the RDN1 locus in Saccharomyces cerevisiae can be transcribed by RNA polymerase (Pol) II in addition to Pol I, but Pol II transcription is usually silenced. The deletion of RRN9...

Regulation of rRNA Synthesis by TATA-Binding Protein-Associated Factor Mot1▿

Dasgupta, Arindam, Sprouse, Rebekka O., French, Sarah, Aprikian, Pavel, Hontz, Robert, Juedes, Sarah A., ...

Mot1 is an essential, conserved, TATA-binding protein (TBP)-associated factor in Saccharomyces cerevisiae with well-established roles in the global control of RNA polymerase II (Pol II)...

Mrd1p binds to pre-rRNA early during transcription independent of U3 snoRNA and is required for compaction of the pre-rRNA into small subunit processomes

Segerstolpe, Åsa, Lundkvist, Pär, Osheim, Yvonne N., Beyer, Ann L., Wieslander, Lars

In Saccharomyces cerevisiae, synthesis of the small ribosomal subunit requires assembly of the 35S pre-rRNA into a 90S preribosomal complex. SnoRNAs, including U3 snoRNA, and many trans-acting...

Visual Analysis of the Yeast 5S rRNA Gene Transcriptome: Regulation and Role of La Protein▿ †

French, Sarah L., Osheim, Yvonne N., Schneider, David A., Sikes, Martha L., Fernandez, Cesar F., Copela, Laura A., ...

5S rRNA genes from Saccharomyces cerevisiae were examined by Miller chromatin spreading, representing the first quantitative analysis of RNA polymerase III genes in situ by electron microscopy. These...

TOR regulates the subcellular distribution of DIM2, a KH domain protein required for cotranscriptional ribosome assembly and pre-40S ribosome export

Vanrobays, Emmanuel, Leplus, Alexis, Osheim, Yvonne N., Beyer, Ann L., Wacheul, Ludivine, Lafontaine, Denis L.J.

Eukaryotic ribosome synthesis is a highly dynamic process that involves the transient association of scores of trans-acting factors to nascent pre-ribosomes. Many ribosome synthesis factors are...

Transcription of Multiple Yeast Ribosomal DNA Genes Requires Targeting of UAF to the Promoter by Uaf30▿ †

Hontz, Robert D., French, Sarah L., Oakes, Melanie L., Tongaonkar, Prasad, Nomura, Masayasu, Beyer, Ann L., ...

Upstream activating factor (UAF) is a multisubunit complex that functions in the activation of ribosomal DNA (rDNA) transcription by RNA polymerase I (Pol I). Cells lacking the Uaf30 subunit of UAF...

The Paf1 complex is required for efficient transcription elongation by RNA polymerase I

Zhang, Yinfeng, Sikes, Martha L., Beyer, Ann L., Schneider, David A.

Regulation of RNA polymerase I (Pol I) transcription is critical for controlling ribosome synthesis. Most previous investigations into Pol I transcription regulation have focused on transcription...