Sahin, Özgür, Fröhlich, Holger, Löbke, Christian, Korf, Ulrike, Burmester, Sara, Majety, Meher, ...
Abstract Background In breast cancer, overexpression of the transmembrane tyrosine kinase ERBB2 is an adverse prognostic marker, and occurs in almost 30% of the patients. For therapeutic...
Judith M. Boer, Wolfgang K. Huber, Holger Sültmann, Friederike Wilmer, Anja Von Heydebreck, Stefan Haas, ...
We investigated the changes in gene expression accompanying the development and progression of kidney cancer by use of 31,500-element complementary DNA arrays. We measured expression profiles for...
Analysis (A. Poustka) DKFZ Heidelberg Acknowledgements (2008)
Wolfgang Huber, Anja Von Heydebreck, Günther Sawitzki, Holger Sültmann, Klaus Steiner, Markus Vogt, ...
First analysis steps o quality control and optimization o calibration and error modeling o data transformations
Automated production of recombinant human proteins as resource for proteome research (2008)
Kohl, Thorsten, Schmidt, Christian, Wiemann, Stefan, Poustka, Annemarie, Korf, Ulrike
Abstract Background An arbitrary set of 96 human proteins was selected and tested to set-up a fully automated protein production strategy, covering all steps from DNA preparation to protein...
Extending pathways based on gene lists using InterPro domain signatures (2008)
Hahne, Florian, Mehrle, Alexander, Arlt, Dorit, Poustka, Annemarie, Wiemann, Stefan, Beissbarth, Tim
Abstract Background High-throughput technologies like functional screens and gene expression analysis produce extended lists of candidate genes. Gene-Set Enrichment Analysis is a commonly used and...
Renner, Marcus, Bergmann, Gaby, Krebs, Inge, End, Caroline, Lyer, Stefan, Hilberg, Frank, ...
BACKGROUND & AIMS: Impaired mucosal defense plays an important role in the pathogenesis of Crohn's disease (CD), one of the main subtypes of inflammatory bowel disease (IBD). Deleted in malignant...
The full-ORF clone resource of the German cDNA Consortium (2007)
Bechtel, Stephanie, Rosenfelder, Heiko, Duda, Anny, Schmidt, Christian, Ernst, Ute, Wellenreuther, Ruth, ...
Abstract Background With the completion of the human genome sequence the functional analysis and characterization of the encoded proteins has become the next urging challenge in the post-genome era....
Large scale statistical inference of signaling pathways from RNAi and microarray data (2007)
Froehlich, Holger, Fellmann, Mark, Sueltmann, Holger, Poustka, Annemarie, Beissbarth, Tim
Abstract Background The advent of RNA interference techniques enables the selective silencing of biologically interesting genes in an efficient way. In combination with DNA microarray technology this...
Müller, Hanna, End, Caroline, Renner, Marcus, Helmke, Burkhard M, Gassler, Nikolaus, Weiss, Christel, ...
Abstract Background Deleted in Malignant Brain Tumors 1 (DMBT1) is a secreted scavenger receptor cysteine-rich protein that binds various bacteria and is thought to participate in innate pulmonary...
Fröhlich, Holger, Speer, Nora, Poustka, Annemarie, Beißbarth, Tim
Abstract Background With the increased availability of high throughput data, such as DNA microarray data, researchers are capable of producing large amounts of biological data. During the analysis of...
BMC Genomics Methodology article The full-ORF clone resource of the German cDNA Consortium (2007)
Stephanie Bechtel, Heiko Rosenfelder, Anny Duda, Christian Peter Schmidt, Ute Ernst, Ruth Wellenreuther, ...
© 2007 Bechtel et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License
CAFTAN: a tool for fast mapping, and quality assessment of cDNAs (2006)
Del Val, Coral, Kuryshev, Vladimir, Glatting, Karl-Heinz, Ernst, Peter, Hotz-Wagenblatt, Agnes, Poustka, Annemarie, ...
Abstract Background The German cDNA Consortium has been cloning full length cDNAs and continued with their exploitation in protein localization experiments and cellular assays. However, the efficient...
Hahne, Florian, Arlt, Dorit, Sauermann, Mamatha, Majety, Meher, Poustka, Annemarie, Wiemann, Stefan, ...
Abstract Highthroughput cell-based assays with flow cytometric readout provide a powerful technique for identifying components of biologic pathways and their interactors. Interpretation of these...
The 3of5 web application for complex and comprehensive pattern matching in protein sequences (2006)
Seiler, Markus, Mehrle, Alexander, Poustka, Annemarie, Wiemann, Stefan
Abstract Background The identification of patterns in biological sequences is a key challenge in genome analysis and in proteomics. Frequently such patterns are complex and highly variable,...
The systematic functional characterisation of Xq28 genes prioritises candidate disease genes (2006)
Kolb-Kokocinski, Anja, Mehrle, Alexander, Bechtel, Stephanie, Simpson, Jeremy C, Kioschis, Petra, Wiemann, Stefan, ...
Abstract Background Well known for its gene density and the large number of mapped diseases, the human sub-chromosomal region Xq28 has long been a focus of genome research. Over 40 of approximately...
Bmc Bioinformatics, Holger Fröhlich, Nora Speer, Annemarie Poustka, Tim Beißbarth, Biomed Central, ...
Software GOSim – an R-package for computation of information theoretic
The LIFEdb database in 2006 (2006)
Er Mehrle, Heiko Rosenfelder, Coral Del Val, Dorit Arlt, Florian Hahne, Stephanie Bechtel, ...
LIFEdb
Bmc Bioinformatics, Coral Del Val, Vladimir Yurjevich Kuryshev, Karl-heinz Glatting, Peter Ernst, Agnes Hotz-wagenblatt, ...
Software CAFTAN: a tool for fast mapping, and quality assessment of cDNAs
Wiemann, Stefan, Kolb-Kokocinski, Anja, Poustka, Annemarie
Abstract Background Given the complexity of higher organisms, the number of genes encoded by their genomes is surprisingly small. Tissue specific regulation of expression and splicing are major...
gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking (2005)
Neubrand,Veronika E., Will,Rainer D., Möbius,Wiebke, Poustka,Annemarie, Wiemann,Stefan, Schu,Peter, ...
gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking (2005)
Neubrand, Veronika E., Will, Rainer D., Möbius, Wiebke, Poustka, Annemarie, Wiemann, Stefan, Schu, Peter, ...
Sueltmann, Holger, Von Heydebreck, Anja, Huber, Wolfgang, Kuner, Ruprecht, Buness, Andreas, Vogt, Markus, ...
Current diagnosis of renal cancer consists of histopathologic examination of tissue sections and classification into tumor stages and grades of malignancy. Until recently, molecular differences...
arrayMagic: twocolour cDNA microarray quality control and preprocessing (2005)
Andreas Buness, Wolfgang Huber, Klaus Steiner, Holger Sültmann, Annemarie Poustka
Summary: arrayMagic is a software package for quality control and preprocessing of two-colour cDNA microarray data. The automated analysis pipeline comprises data import, normalization, replica...
A Compendium to Ensure Computational Reproducibility in High-Dimensional Classification Tasks (2004)
Ruschhaupt, Markus, Huber, Wolfgang, Poustka, Annemarie, Mansmann, Ulrich
We demonstrate a concept and implementation of a compendium for the classification of high-dimensional data from microarray gene expression profiles. A compendium is an interactive document that...
A Compendium to Ensure Computational Reproducibility in High-Dimensional Classification Tasks (2004)
Ruschhaupt, Markus, Huber, Wolfgang, Poustka, Annemarie, Mansmann, Ulrich
We demonstrate a concept and implementation of a compendium for the classification of high-dimensional data from microarray gene expression profiles. A compendium is an interactive document that...
A Compendium to Ensure Computational Reproducibility in High-Dimensional Classification Tasks (2004)
Ruschhaupt, Markus, Huber, Wolfgang, Poustka, Annemarie, Mansmann, Ulrich
We demonstrate a concept and implementation of a compendium for the classification of high-dimensional data from microarray gene expression profiles. A compendium is an interactive document that...
A Compendium to Ensure Computational Reproducibility in High-Dimensional Classification Tasks (2004)
Ruschhaupt, Markus, Huber, Wolfgang, Poustka, Annemarie, Mansmann, Ulrich
We demonstrate a concept and implementation of a compendium for the classification of high-dimensional data from microarray gene expression profiles. A compendium is an interactive document that...
Wellenreuther, Ruth, Schupp, Ingo, Poustka, Annemarie, Wiemann, Stefan
Abstract Background cDNA libraries are widely used to identify genes and splice variants, and as a physical resource for full-length clones. Conventionally-generated cDNA libraries contain a high...
Integrative Annotation of 21,037 Human Genes Validated by Full-Length cDNA Clones (2004)
Tadashi Imanishi, Takeshi Itoh, Yutaka Suzuki, Claire O'Donovan, Satoshi Fukuchi, Kanako O. Koyanagi, ...
An international team has systematically validated and annotated just over 21,000 human genes using full-length cDNA, thereby providing a valuable new resource for the human genetics community.
Integrative Annotation of 21,037 Human Genes Validated by Full-Length cDNA Clones (2004)
Tadashi Imanishi, Takeshi Itoh, Yutaka Suzuki, Claire O'Donovan, Satoshi Fukuchi, Kanako O. Koyanagi, ...
The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this...
Schneider, Jörg, Buneß, Andreas, Huber, Wolfgang, Volz, Joachim, Kioschis, Petra, Hafner, Mathias, ...
Abstract Background The requirement of a large amount of high-quality RNA is a major limiting factor for microarray experiments using biopsies. An average microarray experiment requires 10–100 μg...
Thioredoxin, a regulator of gene expression (2004)
Kontou,Maria, Will,Rainer D., Adelfalk,Caroline, Wittig,Rainer, Poustka,Annemarie, Hirsch-Kauffmann,Monica, ...
Cancer cells have high levels of thioredoxin (Trx) and of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Cells from patients with the cancer-prone disease Fanconi anemia (FA) exhibit reduced Trx...
Thioredoxin, a regulator of gene expression (2004)
Kontou, Maria, Will, Rainer D., Adelfalk, Caroline, Wittig, Rainer, Poustka, Annemarie, Hirsch-Kauffmann, Monica, ...
Cancer cells have high levels of thioredoxin (Trx) and of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Cells from patients with the cancer-prone disease Fanconi anemia (FA) exhibit reduced Trx...
Parameter estimation for the calibration and variance stabilization of microarray data (2003)
Huber,Wolfgang, Von Heydebreck,Anja, Sültmann,Holger, Poustka,Annemarie, Vingron,Martin
We derive and validate an estimator for the parameters of a transformation for the joint calibration (normalization) and variance stabilization of microarray intensity data. With this, the variances...
Parameter estimation for the calibration and variance stabilization of microarray data (2003)
Huber, Wolfgang, Von Heydebreck, Anja, Sueltmann, Holger, Poustka, Annemarie, Vingron, Martin
We derive and validate an estimator for the parameters of a transformation for the joint calibration (normalization) and variance stabilization of microarray intensity data. With this, the variances...
Parameter estimation for the calibration and variance stabilization of microarray data (2003)
Huber, Wolfgang, Von Heydebreck, Anja, Sueltmann, Holger, Poustka, Annemarie, Vingron, Martin
We derive and validate an estimator for the parameters of a transformation for the joint calibration (normalization) and variance stabilization of microarray intensity data. With this, the variances...
Parameter estimation for the calibration and variance stabilization of microarray data (2003)
Huber, Wolfgang, Von Heydebreck, Anja, Sueltmann, Holger, Poustka, Annemarie, Vingron, Martin
We derive and validate an estimator for the parameters of a transformation for the joint calibration (normalization) and variance stabilization of microarray intensity data. With this, the variances...
Parameter estimation for the calibration and variance stabilization of microarray data (2003)
Huber, Wolfgang, Von Heydebreck, Anja, Sueltmann, Holger, Poustka, Annemarie, Vingron, Martin
We derive and validate an estimator for the parameters of a transformation for the joint calibration (normalization) and variance stabilization of microarray intensity data. With this, the variances...
Parameter estimation for the calibration and variance stabilization of microarray data (2003)
Huber, Wolfgang, Von Heydebreck, Anja, Sültmann, Holger, Poustka, Annemarie, Vingron, Martin
We derive and validate an estimator for the parameters of a transformation for the joint calibration (normalization) and variance stabilization of microarray intensity data. With this, the variances...
Huber,Wolfgang, Von Heydebreck,Anja, Sültmann,Holger, Poustka,Annemarie, Vingron,Martin
We introduce a statistical model for microarray gene expression data that comprises data calibration, the quantification of differential expression, and the quantification of measurement error. In...
Huber, Wolfgang, Von Heydebreck, Anja, Sültmann, Holger, Poustka, Annemarie, Vingron, Martin
We introduce a statistical model for microarray gene expression data that comprises data calibration, the quantification of differential expression, and the quantification of measurement error. In...
Mollenhauer, Jan, Herbertz, Stephan, Helmke, Burkhard, Kollender, Gaby, Krebs, Inge, Madsen, Jens, ...
Deleted in Malignant Brain Tumors 1 (DMBT1) has been proposed as a candidate tumor suppressor gene for brain, lung, and digestive tract cancer. In particular, alterations of the gene and/or a loss of...
The fragile X syndrome : from phenotype to gene / (1993)
Enth. 10 Sonderabdr. aus verschiedenen Zeitschr.
Homozygous deletion in Wilms tumours of a zinc-finger gene identified by chromosome jumping (1990)
Gessler, Manfred, Poustka, Annemarie, Cavenee, Webster, Neve, Rachael L., Orkin, Stuart H., Bruns, Gail A.
Cloning of gp-340, a putative opsonin receptor for lung surfactant protein D
Holmskov, Uffe, Mollenhauer, Jan, Madsen, Jens, Vitved, Lars, Grønlund, Jørn, Tornøe, Ida, ...
Surfactant protein D (SP-D) is an oligomeric C type lectin that promotes phagocytosis by binding to microbial surface carbohydrates. A 340-kDa glycoprotein (gp-340) has been shown to bind SP-D in the...
Wiemann, Stefan, Weil, Bernd, Wellenreuther, Ruth, Gassenhuber, Johannes, Glassl, Sabine, Ansorge, Wilhelm, ...
With the complete human genomic sequence being unraveled, the focus will shift to gene identification and to the functional analysis of gene products. The generation of a set of cDNAs, both sequences...
Boer, Judith M., Huber, Wolfgang K., Sültmann, Holger, Wilmer, Friederike, Von Heydebreck, Anja, Haas, Stefan, ...
We investigated the changes in gene expression accompanying the development and progression of kidney cancer by use of 31,500-element complementary DNA arrays. We measured expression profiles for...
Bannasch, Detlev, Mehrle, Alexander, Glatting, Karl-Heinz, Pepperkok, Rainer, Poustka, Annemarie, Wiemann, Stefan
We have implemented LIFEdb (http://www.dkfz.de/LIFEdb) to link information regarding novel human full-length cDNAs generated and sequenced by the German cDNA Consortium with functional information on...
High-throughput protein analysis integrating bioinformatics and experimental assays
Del Val, Coral, Mehrle, Alexander, Falkenhahn, Mechthild, Seiler, Markus, Glatting, Karl-Heinz, Poustka, Annemarie, ...
The wealth of transcript information that has been made publicly available in recent years requires the development of high-throughput functional genomics and proteomics approaches for its analysis....
Integrative Annotation of 21,037 Human Genes Validated by Full-Length cDNA Clones
Imanishi, Tadashi, Itoh, Takeshi, Suzuki, Yutaka, O'Donovan, Claire, Fukuchi, Satoshi, Koyanagi, Kanako O, ...
The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this...
Schneider, Jörg, Buneß, Andreas, Huber, Wolfgang, Volz, Joachim, Kioschis, Petra, Hafner, Mathias, ...
Starkuviene, Vytaute, Liebel, Urban, Simpson, Jeremy C., Erfle, Holger, Poustka, Annemarie, Wiemann, Stefan, ...
Here we describe the establishment of microscope-based functional screening assays in intact cells that allow us to systematically identify new proteins involved in secretory membrane traffic, and...
From ORFeome to Biology: A Functional Genomics Pipeline
Wiemann, Stefan, Arlt, Dorit, Huber, Wolfgang, Wellenreuther, Ruth, Schleeger, Simone, Mehrle, Alexander, ...
As several model genomes have been sequenced, the elucidation of protein function is the next challenge toward the understanding of biological processes in health and disease. We have generated a...
γ-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking
Neubrand, Veronika E, Will, Rainer D, Möbius, Wiebke, Poustka, Annemarie, Wiemann, Stefan, Schu, Peter, ...
A novel peripheral membrane protein (2c18) that interacts directly with the gamma ‘ear' domain of the adaptor protein complex 1 (AP-1) in vitro and in vivo is described. Ultrastructural analysis...
Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing
Simpson, Jeremy C., Wellenreuther, Ruth, Poustka, Annemarie, Pepperkok, Rainer, Wiemann, Stefan
As a first step towards a more comprehensive functional characterization of cDNAs than bioinformatic analysis, which can only make functional predictions for about half of the cDNAs sequenced, we...
Alternative pre-mRNA processing regulates cell-type specific expression of the IL4l1 and NUP62 genes
Mehrle, Alexander, Rosenfelder, Heiko, Schupp, Ingo, Del Val, Coral, Arlt, Dorit, Hahne, Florian, ...
LIFEdb () integrates data from large-scale functional genomics assays and manual cDNA annotation with bioinformatics gene expression and protein analysis. New features of LIFEdb include (i) an...
The systematic functional characterisation of Xq28 genes prioritises candidate disease genes
Kolb-Kokocinski, Anja, Mehrle, Alexander, Bechtel, Stephanie, Simpson, Jeremy C, Kioschis, Petra, Wiemann, Stefan, ...
The 3of5 web application for complex and comprehensive pattern matching in protein sequences
Seiler, Markus, Mehrle, Alexander, Poustka, Annemarie, Wiemann, Stefan
CAFTAN: a tool for fast mapping, and quality assessment of cDNAs
Del Val, Coral, Kuryshev, Vladimir Yurjevich, Glatting, Karl-Heinz, Ernst, Peter, Hotz-Wagenblatt, Agnes, Poustka, Annemarie, ...
Wöhrle, Doris, Kotzot, Dieter, Hirst, Mark C., Manca, Antonella, Korn, Bernhard, Schmidt, Angela, ...
A gene designated “FMR-1” has been isolated at the fragile-X locus. One exon of this gene is carried on a 5.1-kb EcoRI fragment that exhibits length variation in fragile-X patients because of...
Cloning of gp-340, a putative opsonin receptor for lung surfactant protein D
Holmskov, Uffe, Mollenhauer, Jan, Madsen, Jens, Vitved, Lars, Grønlund, Jørn, Tornøe, Ida, ...
Surfactant protein D (SP-D) is an oligomeric C type lectin that promotes phagocytosis by binding to microbial surface carbohydrates. A 340-kDa glycoprotein (gp-340) has been shown to bind SP-D in the...
Wiemann, Stefan, Weil, Bernd, Wellenreuther, Ruth, Gassenhuber, Johannes, Glassl, Sabine, Ansorge, Wilhelm, ...
With the complete human genomic sequence being unraveled, the focus will shift to gene identification and to the functional analysis of gene products. The generation of a set of cDNAs, both sequences...
Boer, Judith M., Huber, Wolfgang K., Sültmann, Holger, Wilmer, Friederike, Von Heydebreck, Anja, Haas, Stefan, ...
We investigated the changes in gene expression accompanying the development and progression of kidney cancer by use of 31,500-element complementary DNA arrays. We measured expression profiles for...
Bannasch, Detlev, Mehrle, Alexander, Glatting, Karl-Heinz, Pepperkok, Rainer, Poustka, Annemarie, Wiemann, Stefan
We have implemented LIFEdb (http://www.dkfz.de/LIFEdb) to link information regarding novel human full-length cDNAs generated and sequenced by the German cDNA Consortium with functional information on...
High-throughput protein analysis integrating bioinformatics and experimental assays
Del Val, Coral, Mehrle, Alexander, Falkenhahn, Mechthild, Seiler, Markus, Glatting, Karl-Heinz, Poustka, Annemarie, ...
The wealth of transcript information that has been made publicly available in recent years requires the development of high-throughput functional genomics and proteomics approaches for its analysis....
Klauck, Sabine M., Lindsay, Susan, Beyer, Kim S., Splitt, Miranda, Burn, John, Poustka, Annemarie
We report here the genetic cause of the X-linked syndrome of psychosis, pyramidal signs, and macro-orchidism (PPM-X) in a three-generation family manifesting the disorder as a mutation in the...
Integrative Annotation of 21,037 Human Genes Validated by Full-Length cDNA Clones
Imanishi, Tadashi, Itoh, Takeshi, Suzuki, Yutaka, O'Donovan, Claire, Fukuchi, Satoshi, Koyanagi, Kanako O, ...
The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this...
Schneider, Jörg, Buneß, Andreas, Huber, Wolfgang, Volz, Joachim, Kioschis, Petra, Hafner, Mathias, ...
Starkuviene, Vytaute, Liebel, Urban, Simpson, Jeremy C., Erfle, Holger, Poustka, Annemarie, Wiemann, Stefan, ...
Here we describe the establishment of microscope-based functional screening assays in intact cells that allow us to systematically identify new proteins involved in secretory membrane traffic, and...
From ORFeome to Biology: A Functional Genomics Pipeline
Wiemann, Stefan, Arlt, Dorit, Huber, Wolfgang, Wellenreuther, Ruth, Schleeger, Simone, Mehrle, Alexander, ...
As several model genomes have been sequenced, the elucidation of protein function is the next challenge toward the understanding of biological processes in health and disease. We have generated a...
γ-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking
Neubrand, Veronika E, Will, Rainer D, Möbius, Wiebke, Poustka, Annemarie, Wiemann, Stefan, Schu, Peter, ...
A novel peripheral membrane protein (2c18) that interacts directly with the gamma ‘ear' domain of the adaptor protein complex 1 (AP-1) in vitro and in vivo is described. Ultrastructural analysis...
Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing
Simpson, Jeremy C., Wellenreuther, Ruth, Poustka, Annemarie, Pepperkok, Rainer, Wiemann, Stefan
As a first step towards a more comprehensive functional characterization of cDNAs than bioinformatic analysis, which can only make functional predictions for about half of the cDNAs sequenced, we...
Alternative pre-mRNA processing regulates cell-type specific expression of the IL4l1 and NUP62 genes
Mehrle, Alexander, Rosenfelder, Heiko, Schupp, Ingo, Del Val, Coral, Arlt, Dorit, Hahne, Florian, ...
LIFEdb () integrates data from large-scale functional genomics assays and manual cDNA annotation with bioinformatics gene expression and protein analysis. New features of LIFEdb include (i) an...
The systematic functional characterisation of Xq28 genes prioritises candidate disease genes
Kolb-Kokocinski, Anja, Mehrle, Alexander, Bechtel, Stephanie, Simpson, Jeremy C, Kioschis, Petra, Wiemann, Stefan, ...
The 3of5 web application for complex and comprehensive pattern matching in protein sequences
Seiler, Markus, Mehrle, Alexander, Poustka, Annemarie, Wiemann, Stefan
CAFTAN: a tool for fast mapping, and quality assessment of cDNAs
Del Val, Coral, Kuryshev, Vladimir Yurjevich, Glatting, Karl-Heinz, Ernst, Peter, Hotz-Wagenblatt, Agnes, Poustka, Annemarie, ...
Wöhrle, Doris, Kotzot, Dieter, Hirst, Mark C., Manca, Antonella, Korn, Bernhard, Schmidt, Angela, ...
A gene designated “FMR-1” has been isolated at the fragile-X locus. One exon of this gene is carried on a 5.1-kb EcoRI fragment that exhibits length variation in fragile-X patients because of...
Hahne, Florian, Arlt, Dorit, Sauermann, Mamatha, Majety, Meher, Poustka, Annemarie, Wiemann, Stefan, ...
A software tool for the analysis of high-throughput cell-based assays is presented.
Combinatorial RNAi for quantitative protein network analysis
Sahin, Özgür, Löbke, Christian, Korf, Ulrike, Appelhans, Heribert, Sültmann, Holger, Poustka, Annemarie, ...
The elucidation of cross-talk events between intersecting signaling pathways is one main challenge in biological research. The complexity of protein networks, composed of different pathways, requires...
Blackburn, Anneke C., Hill, Linda Z., Roberts, Amy L., Wang, Jun, Aud, Dee, Jung, Jimmy, ...
Low-penetrance breast cancer susceptibility alleles seem to play a significant role in breast cancer risk but are difficult to identify in human cohorts. A genetic screen of 176 N2 backcross progeny...
Müller, Hanna, End, Caroline, Renner, Marcus, Helmke, Burkhard M, Gassler, Nikolaus, Weiss, Christel, ...
The full-ORF clone resource of the German cDNA Consortium
Bechtel, Stephanie, Rosenfelder, Heiko, Duda, Anny, Schmidt, Christian Peter, Ernst, Ute, Wellenreuther, Ruth, ...
Extending pathways based on gene lists using InterPro domain signatures
Hahne, Florian, Mehrle, Alexander, Arlt, Dorit, Poustka, Annemarie, Wiemann, Stefan, Beissbarth, Tim
Large scale statistical inference of signaling pathways from RNAi and microarray data
Froehlich, Holger, Fellmann, Mark, Sueltmann, Holger, Poustka, Annemarie, Beissbarth, Tim
Automated production of recombinant human proteins as resource for proteome research
Kohl, Thorsten, Schmidt, Christian, Wiemann, Stefan, Poustka, Annemarie, Korf, Ulrike
A Compendium to Ensure Computational Reproducibility in High-Dimensional Classification Tasks
Markus Ruschhaupt, Wolfgang Huber, Annemarie Poustka, Ulrich Mansmann
We demonstrate a concept and implementation of a compendium for the classification of high-dimensional data from microarray gene expression profiles. A compendium is an interactive document that...
Parameter estimation for the calibration and variance stabilization of microarray data
Wolfgang Huber, Anja Von Heydebreck, Holger Sueltmann, Annemarie Poustka, Martin Vingron
We derive and validate an estimator for the parameters of a transformation for the joint calibration (normalization) and variance stabilization of microarray intensity data. With this, the variances...
Predicting pathway membership via domain signatures
Fröhlich, Holger, Fellmann, Mark, Sültmann, Holger, Poustka, Annemarie, Beißbarth, Tim
Motivation: Functional characterization of genes is of great importance for the understanding of complex cellular processes. Valuable information for this purpose can be obtained from pathway...
Fröhlich, Holger, Fellmann, Mark, Sültmann, Holger, Poustka, Annemarie, Beissbarth, Tim
Motivation: Targeted interventions using RNA interference in combination with the measurement of secondary effects with DNA microarrays can be used to computationally reverse engineer features of...