Anthony A. Holder

Characterization of the repertoire diversity of the Plasmodium falciparum stevormultigene family in laboratory and field isolates (2009)

Blythe, Jane E, Niang, Makhtar, Marsh, Kevin, Holder, Anthony A, Langhorne, Jean, Preiser, Peter R

Abstract Background The evasion of host immune response by the human malaria parasite Plasmodium falciparum has been linked to expression of a range of variable antigens on the infected erythrocyte...

Epigenetic Silencing of Plasmodium falciparum Genes Linked to Erythrocyte Invasion (2007)

Alfred Cortés, Celine Carret, Osamu Kaneko, Alasdair Ivens, Anthony A. Holder

The process of erythrocyte invasion by merozoites of Plasmodium falciparum involves multiple steps, including the formation of a moving junction between parasite and host cell, and it is...

The Importance of Human FcγRI in Mediating Protection to Malaria (2007)

Richard S. McIntosh, Jianguo Shi, Richard M. Jennings, Jonathan C. Chappel, Tim Smith, ...

The success of passive immunization suggests that antibody-based therapies will be effective at controlling malaria. We describe the development of fully human antibodies specific for Plasmodium...

Importance of human FcyRI in mediating protection to malaria (2007)

McIntosh, Richard S., Shi, Jianguo, Jennings, Richard M., Chappel, Jonathan C., Smith, Tim, ...

The success of passive immunization suggests that antibody-based therapies will be effective at controlling malaria. We describe the development of fully human antibodies specific for Plasmodium...

The importance of human FcyRI in mediating protection to malaria (2007)

McIntosh, Richard S., Shi, Jianguo, Jennings, Richard M., Chappel, Jonathan C., Smith, Tim, ...

The success of passive immunization suggests that antibody-based therapies will be effective at controlling malaria. We describe the development of fully human antibodies specific for Plasmodium...

Global Analysis of Transcript and Protein Levels Across the Plasmodium falciparum Life Cycle (2006)

LeRoch, Karine G., Johnson, Jeffrey R., Florens, Laurence, Zhou, Yingyao, Santrosyan, Audrey, Grainger, Munira, ...

To investigate the role of post-transcriptional controls in the regulation of protein expression for the malaria parasite, Plasmodium falciparum, we have compared mRNA transcript and protein...

FUNCTIONAL CHARACTERIZATION OF AN LCCL–LECTIN DOMAIN CONTAINING PROTEIN FAMILY IN PLASMODIUM BERGHEI (2004)

Holly E. Trueman, J. Dale Raine, Laurence Florens, Johannes T. Dessens, Jacqui Mendoza, Jeffrey Johnson, ...

Using bioinformatic, proteomic, immunofluorescence, and genetic cross methods, we have functionally characterized a family of putative parasite ligands as potential mediators of cell–cell...

Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle (2004)

Le Roch, Karine G., Johnson, Jeffrey R., Florens, Laurence, Zhou, Yingyao, Santrosyan, Andrey, Grainger, Munira, ...

To investigate the role of post-transcriptional controls in the regulation of protein expression for the malaria parasite, Plasmodium falciparum, we have compared mRNA transcript and protein...

Cellular immune response to Plasmodium falciparumafter pregnancy is related to previous placental infection and parity (2002)

Fievet, Nadine, Tami, Germaine, Maubert, Bertrand, Moussa, Marlène, Shaw, Ian K, Cot, Michel, ...

Abstract Background Malaria in pregnancy is characterised by the sequestration of Plasmodium falciparum -infected erythrocytes in placental intervillous spaces. Placental parasites express a specific...

Linkage of Exogenous T-cell Epitopes to the 19-Kilodalton Region of Plasmodium yoelii Merozoite Surface Protein 1 (MSP119) Can Enhance Protective Immunity against Malaria and Modulate the Immunoglobulin Subclass Response to MSP119

Ahlborg, Niklas, Ling, Irene T., Holder, Anthony A., Riley, Eleanor M.

The degree of protection against Plasmodium yoelii asexual blood stages induced by immunization of mice with the 19-kDa region of merozoite surface protein 1 (MSP119) is H-2 dependent. As a strategy...

Immunization with Parasite-Derived Apical Membrane Antigen 1 or Passive Immunization with a Specific Monoclonal Antibody Protects BALB/c Mice against Lethal Plasmodium yoelii yoelii YM Blood-Stage Infection

Narum, David L., Ogun, Solabomi A., Thomas, Alan W., Holder, Anthony A.

We have purified apical merozoite antigen 1 (AMA-1) from extracts of red blood cells infected with the rodent malaria parasite Plasmodium yoelii yoelii YM. When used to immunize mice, the protein...

Antibody Recognition of Rodent Malaria Parasite Antigens Exposed at the Infected Erythrocyte Surface: Specificity of Immunity Generated in Hyperimmune Mice

Mota, Maria M., Brown, K. Neil, Do Rosário, Virgilio E., Holder, Anthony A., Jarra, William

In regions where malaria is endemic, inhabitants remain susceptible to repeated reinfection as they develop and maintain clinical immunity. This immunity includes responses to surface-exposed...

Plasmodium falciparum Homologue of the Genes for Plasmodium vivax and Plasmodium yoelii Adhesive Proteins, Which Is Transcribed but Not Translated

Taylor, Helen M., Triglia, Tony, Thompson, Jenny, Sajid, Mohammed, Fowler, Ruth, Wickham, Mark E., ...

The 235-kDa family of rhoptry proteins in Plasmodium yoelii and the two reticulocyte binding proteins of P. vivax comprise a family of proteins involved in host cell selection and erythrocyte...

Plasmodium chabaudi-Infected Erythrocytes Adhere to CD36 and Bind to Microvascular Endothelial Cells in an Organ-Specific Way

Mota, Maria M., Jarra, William, Hirst, Elizabeth, Patnaik, Pradeep K., Holder, Anthony A.

Adherence of erythrocytes infected with Plasmodium falciparum to microvascular endothelial cells (sequestration) is considered to play an important role in parasite virulence and pathogenesis....

Passive Immunization with Antibodies against Three Distinct Epitopes on Plasmodium yoelii Merozoite Surface Protein 1 Suppresses Parasitemia

Spencer Valero, Lilian M., Ogun, Solabomi A., Fleck, Suzanne L., Ling, Irene T., Scott-Finnigan, Terry J., Blackman, Michael J., ...

We have produced monoclonal antibodies against Plasmodium yoelii merozoite surface protein 1 (MSP-1) and have assessed their ability to suppress blood stage parasitemia by passive immunization. Six...

Acute Plasmodium chabaudi chabaudi Malaria Infection Induces Antibodies Which Bind to the Surfaces of Parasitized Erythrocytes and Promote Their Phagocytosis by Macrophages In Vitro

Mota, Maria M., Brown, K. Neil, Holder, Anthony A., Jarra, William

CBA/Ca mice infected with 5 × 104 Plasmodium chabaudi chabaudi AS-parasitized erythrocytes experience acute but self-limiting infections of relatively short duration. Parasitemia peaks (∼40%...

Protective Immune Responses to the 42-Kilodalton (kDa) Region of Plasmodium yoelii Merozoite Surface Protein 1 Are Induced by the C-Terminal 19-kDa Region but Not by the Adjacent 33-kDa Region

Ahlborg, Niklas, Ling, Irene T., Howard, Wendy, Holder, Anthony A., Riley, Eleanor M.

Vaccination of mice with the 42-kDa region of Plasmodium yoelii merozoite surface protein 1 (MSP142) or its 19-kDa C-terminal processing product (MSP119) can elicit protective antibody responses in...

The Human Immune Response to Plasmodium falciparum Includes Both Antibodies That Inhibit Merozoite Surface Protein 1 Secondary Processing and Blocking Antibodies

Nwuba, Roseangela I., Sodeinde, Olugbemiro, Anumudu, Chiaka I., Omosun, Yusuf O., Odaibo, Alexander B., Holder, Anthony A., ...

Malaria merozoite surface protein 1 (MSP1) is cleaved in an essential step during erythrocyte invasion. The responses of children to natural malaria infection included antibodies that inhibit this...

Variation in the Expression of a Plasmodium falciparum Protein Family Implicated in Erythrocyte Invasion

Taylor, Helen M., Grainger, Munira, Holder, Anthony A.

The PfRH protein family of Plasmodium falciparum is implicated in erythrocyte invasion. Here we report variations in the sequence, transcription, and protein expression of four different members of...

Fine Specificity of Serum Antibodies to Plasmodium falciparum Merozoite Surface Protein, PfMSP-119, Predicts Protection from Malaria Infection and High-Density Parasitemia†

Okech, Brenda A., Corran, Patrick H., Todd, James, Joynson-Hicks, Amy, Uthaipibull, Chairat, Egwang, Thomas G., ...

Antibodies to the C terminus of the Plasmodium falciparum merozoite surface protein, PfMSP-119, may inhibit merozoite invasion or block the effects of inhibitory antibodies. Here, using a competition...

Amino-Acid Sequence of NADP-Specific Glutamate Dehydrogenase of Neurospora crassa

Wootton, John C., Chambers, Geoffrey K., Holder, Anthony A., Baron, Andrew J., Taylor, John G., Fincham, John R. S., ...

A tentative primary structure of the NADP-specific glutamate dehydrogenase [L-glutamate: NADP oxidoreductase (deaminating), EC 1.4.1.4] from Neurospora crassa has been determined. The proposed...

Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle

Le Roch, Karine G., Johnson, Jeffrey R., Florens, Laurence, Zhou, Yingyao, Santrosyan, Andrey, Grainger, Munira, ...

To investigate the role of post-transcriptional controls in the regulation of protein expression for the malaria parasite, Plasmodium falciparum, we have compared mRNA transcript and protein...

Immunoglobulin G Antibodies to Merozoite Surface Antigens Are Associated with Recovery from Chloroquine-Resistant Plasmodium falciparum in Gambian Children

Pinder, Margaret, Sutherland, Colin J., Sisay-Joof, Fatoumatta, Ismaili, Jamila, McCall, Matthew B. B., Ord, Rosalyn, ...

We examined the hypothesis that recovery from uncomplicated malaria in patients carrying drug-resistant Plasmodium falciparum is a measure of acquired functional immunity and may therefore be...

Linkage of Exogenous T-cell Epitopes to the 19-Kilodalton Region of Plasmodium yoelii Merozoite Surface Protein 1 (MSP119) Can Enhance Protective Immunity against Malaria and Modulate the Immunoglobulin Subclass Response to MSP119

Ahlborg, Niklas, Ling, Irene T., Holder, Anthony A., Riley, Eleanor M.

The degree of protection against Plasmodium yoelii asexual blood stages induced by immunization of mice with the 19-kDa region of merozoite surface protein 1 (MSP119) is H-2 dependent. As a strategy...

Immunization with Parasite-Derived Apical Membrane Antigen 1 or Passive Immunization with a Specific Monoclonal Antibody Protects BALB/c Mice against Lethal Plasmodium yoelii yoelii YM Blood-Stage Infection

Narum, David L., Ogun, Solabomi A., Thomas, Alan W., Holder, Anthony A.

We have purified apical merozoite antigen 1 (AMA-1) from extracts of red blood cells infected with the rodent malaria parasite Plasmodium yoelii yoelii YM. When used to immunize mice, the protein...

Antibody Recognition of Rodent Malaria Parasite Antigens Exposed at the Infected Erythrocyte Surface: Specificity of Immunity Generated in Hyperimmune Mice

Mota, Maria M., Brown, K. Neil, Do Rosário, Virgilio E., Holder, Anthony A., Jarra, William

In regions where malaria is endemic, inhabitants remain susceptible to repeated reinfection as they develop and maintain clinical immunity. This immunity includes responses to surface-exposed...

Plasmodium falciparum Homologue of the Genes for Plasmodium vivax and Plasmodium yoelii Adhesive Proteins, Which Is Transcribed but Not Translated

Taylor, Helen M., Triglia, Tony, Thompson, Jenny, Sajid, Mohammed, Fowler, Ruth, Wickham, Mark E., ...

The 235-kDa family of rhoptry proteins in Plasmodium yoelii and the two reticulocyte binding proteins of P. vivax comprise a family of proteins involved in host cell selection and erythrocyte...

Plasmodium chabaudi-Infected Erythrocytes Adhere to CD36 and Bind to Microvascular Endothelial Cells in an Organ-Specific Way

Mota, Maria M., Jarra, William, Hirst, Elizabeth, Patnaik, Pradeep K., Holder, Anthony A.

Adherence of erythrocytes infected with Plasmodium falciparum to microvascular endothelial cells (sequestration) is considered to play an important role in parasite virulence and pathogenesis....

Passive Immunization with Antibodies against Three Distinct Epitopes on Plasmodium yoelii Merozoite Surface Protein 1 Suppresses Parasitemia

Spencer Valero, Lilian M., Ogun, Solabomi A., Fleck, Suzanne L., Ling, Irene T., Scott-Finnigan, Terry J., Blackman, Michael J., ...

We have produced monoclonal antibodies against Plasmodium yoelii merozoite surface protein 1 (MSP-1) and have assessed their ability to suppress blood stage parasitemia by passive immunization. Six...

Acute Plasmodium chabaudi chabaudi Malaria Infection Induces Antibodies Which Bind to the Surfaces of Parasitized Erythrocytes and Promote Their Phagocytosis by Macrophages In Vitro

Mota, Maria M., Brown, K. Neil, Holder, Anthony A., Jarra, William

CBA/Ca mice infected with 5 × 104 Plasmodium chabaudi chabaudi AS-parasitized erythrocytes experience acute but self-limiting infections of relatively short duration. Parasitemia peaks (∼40%...

Protective Immune Responses to the 42-Kilodalton (kDa) Region of Plasmodium yoelii Merozoite Surface Protein 1 Are Induced by the C-Terminal 19-kDa Region but Not by the Adjacent 33-kDa Region

Ahlborg, Niklas, Ling, Irene T., Howard, Wendy, Holder, Anthony A., Riley, Eleanor M.

Vaccination of mice with the 42-kDa region of Plasmodium yoelii merozoite surface protein 1 (MSP142) or its 19-kDa C-terminal processing product (MSP119) can elicit protective antibody responses in...

The Human Immune Response to Plasmodium falciparum Includes Both Antibodies That Inhibit Merozoite Surface Protein 1 Secondary Processing and Blocking Antibodies

Nwuba, Roseangela I., Sodeinde, Olugbemiro, Anumudu, Chiaka I., Omosun, Yusuf O., Odaibo, Alexander B., Holder, Anthony A., ...

Malaria merozoite surface protein 1 (MSP1) is cleaved in an essential step during erythrocyte invasion. The responses of children to natural malaria infection included antibodies that inhibit this...

Variation in the Expression of a Plasmodium falciparum Protein Family Implicated in Erythrocyte Invasion

Taylor, Helen M., Grainger, Munira, Holder, Anthony A.

The PfRH protein family of Plasmodium falciparum is implicated in erythrocyte invasion. Here we report variations in the sequence, transcription, and protein expression of four different members of...

Fine Specificity of Serum Antibodies to Plasmodium falciparum Merozoite Surface Protein, PfMSP-119, Predicts Protection from Malaria Infection and High-Density Parasitemia†

Okech, Brenda A., Corran, Patrick H., Todd, James, Joynson-Hicks, Amy, Uthaipibull, Chairat, Egwang, Thomas G., ...

Antibodies to the C terminus of the Plasmodium falciparum merozoite surface protein, PfMSP-119, may inhibit merozoite invasion or block the effects of inhibitory antibodies. Here, using a competition...

Amino-Acid Sequence of NADP-Specific Glutamate Dehydrogenase of Neurospora crassa

Wootton, John C., Chambers, Geoffrey K., Holder, Anthony A., Baron, Andrew J., Taylor, John G., Fincham, John R. S., ...

A tentative primary structure of the NADP-specific glutamate dehydrogenase [L-glutamate: NADP oxidoreductase (deaminating), EC 1.4.1.4] from Neurospora crassa has been determined. The proposed...

Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle

Le Roch, Karine G., Johnson, Jeffrey R., Florens, Laurence, Zhou, Yingyao, Santrosyan, Andrey, Grainger, Munira, ...

To investigate the role of post-transcriptional controls in the regulation of protein expression for the malaria parasite, Plasmodium falciparum, we have compared mRNA transcript and protein...

Immunoglobulin G Antibodies to Merozoite Surface Antigens Are Associated with Recovery from Chloroquine-Resistant Plasmodium falciparum in Gambian Children

Pinder, Margaret, Sutherland, Colin J., Sisay-Joof, Fatoumatta, Ismaili, Jamila, McCall, Matthew B. B., Ord, Rosalyn, ...

We examined the hypothesis that recovery from uncomplicated malaria in patients carrying drug-resistant Plasmodium falciparum is a measure of acquired functional immunity and may therefore be...

Passive Immunization with a Multicomponent Vaccine against Conserved Domains of Apical Membrane Antigen 1 and 235-Kilodalton Rhoptry Proteins Protects Mice against Plasmodium yoelii Blood-Stage Challenge Infection

Narum, David L., Ogun, Solabomi A., Batchelor, Adrian H., Holder, Anthony A.

During malaria parasite invasion of red blood cells, merozoite proteins bind receptors on the surface of the erythrocyte. Two candidate Plasmodium yoelii adhesion proteins are apical membrane antigen...

The Importance of Human FcγRI in Mediating Protection to Malaria

McIntosh, Richard S, Shi, Jianguo, Jennings, Richard M, Chappel, Jonathan C, Smith, Tim, ...

The success of passive immunization suggests that antibody-based therapies will be effective at controlling malaria. We describe the development of fully human antibodies specific for Plasmodium...

Epigenetic Silencing of Plasmodium falciparum Genes Linked to Erythrocyte Invasion

Cortés, Alfred, Carret, Celine, Kaneko, Osamu, Ivens, Alasdair, Holder, Anthony A

The process of erythrocyte invasion by merozoites of Plasmodium falciparum involves multiple steps, including the formation of a moving junction between parasite and host cell, and it is...

Antibodies that Inhibit Malaria Merozoite Surface Protein–1 Processing and Erythrocyte Invasion Are Blocked by Naturally Acquired Human Antibodies

Guevara Patiño, José A., Holder, Anthony A., McBride, Jana S., Blackman, Michael J.

Merozoite surface protein–1 (MSP-1) of the human malaria parasite Plasmodium falciparum undergoes at least two endoproteolytic cleavage events during merozoite maturation and release, and...

Formation of the Food Vacuole in Plasmodium falciparum: A Potential Role for the 19 kDa Fragment of Merozoite Surface Protein 1 (MSP119)

Dluzewski, Anton R., Ling, Irene T., Hopkins, John M., Grainger, Munira, Margos, Gabriele, Mitchell, Graham H., ...

Plasmodium falciparum Merozoite Surface Protein 1 (MSP1) is synthesized during schizogony as a 195-kDa precursor that is processed into four fragments on the parasite surface. Following a second...

Plasmodium falciparum STEVOR Proteins Are Highly Expressed in Patient Isolates and Located in the Surface Membranes of Infected Red Blood Cells and the Apical Tips of Merozoites▿ †

Blythe, Jane E., Yam, Xue Yan, Kuss, Claudia, Bozdech, Zbynek, Holder, Anthony A., Marsh, Kevin, ...

The human parasite Plasmodium falciparum has the potential to express a vast repertoire of variant proteins on the surface of the infected red blood cell (iRBC). Variation in the expression pattern...

The Motor Complex of Plasmodium falciparum: PHOSPHORYLATION BY A CALCIUM-DEPENDENT PROTEIN KINASE*

Green, Judith L., Rees-Channer, Roxanne R., Howell, Stephen A., Martin, Stephen R., Knuepfer, Ellen, Taylor, Helen M., ...

Calcium-dependent protein kinases (CDPKs) of Apicomplexan parasites are crucial for the survival of the parasite throughout its life cycle. CDPK1 is expressed in the asexual blood stages of the...

Comparative Testing of Six Antigen-Based Malaria Vaccine Candidates Directed Toward Merozoite-Stage Plasmodium falciparum▿

Arnot, David E., Cavanagh, David R., Remarque, Edmond J., Creasey, Alison M., Sowa, Mercy P. K., Morgan, William D., ...

Immunogenicity testing of Plasmodium falciparum antigens being considered as malaria vaccine candidates was undertaken in rabbits. The antigens compared were recombinant baculovirus MSP-119 and five...

Identification and Characterization of the Plasmodium yoelii PyP140/RON4 Protein, an Orthologue of Toxoplasma gondii RON4, Whose Cysteine-Rich Domain Does Not Protect against Lethal Parasite Challenge Infection▿

Narum, David L., Nguyen, Vu, Zhang, Yanling, Glen, Jacqueline, Shimp, Richard L., Lambert, Lynn, ...

Previously, we identified a Plasmodium yoelii YM 140-kDa merozoite protein, designated PyP140, which formed a complex with apical membrane antigen 1 (AMA1). Furthermore, we produced a nonprotective...

Deletion of the Plasmodium falciparum Merozoite Surface Protein 7 Gene Impairs Parasite Invasion of Erythrocytes▿

Kadekoppala, Madhusudan, O'Donnell, Rebecca A., Grainger, Munira, Crabb, Brendan S., Holder, Anthony A.

Merozoite surface proteins have been implicated in the initial attachment to the host red blood cell membrane that begins the process of invasion, an important step in the life cycle of the malaria...