Nguyen, Thanh V, Sacci, John B, De La Vega, Patricia, John, Chandy C, James, Anthony A, Kang, Angray S
Abstract Background MB2 protein is a sporozoite surface antigen on the human malaria parasite Plasmodium falciparum . MB2 was identified by screening a P. falciparum sporozoite cDNA expression...
A transcriptome analysis of the Aedes aegypti vitellogenic fat body (2006)
Fabiana M. Feitosa, Eric Calvo, Emilio F. Merino, Alan M. Durham, Anthony A. James, ...
Aedes (Stegomyia) aegypti is an important dengue vector in tropical and subtropical zones throughout the world. A transcriptome of Ae. aegypti vitellogenic fat bodies is described here. The fat body...
Dissanayake, Sumudu N, Marinotti, Osvaldo, Ribeiro, Jose Marcos C, James, Anthony A
Abstract Background The completed sequence of the Anopheles gambiae genome has enabled genome-wide analyses of gene expression and regulation in this principal vector of human malaria. These...
Genome-wide analysis of gene expression in adult Anopheles gambiae (2006)
Marinotti, Osvaldo, Calvo, Eric, Nguyen, Quang K, Dissanayake, Sumudu, Ribeiro, Jose M.C., James, Anthony A.
With their genome sequenced, Anopheles gambiae mosquitoes now serve as a powerful tool for basic research in comparative, evolutionary and developmental biology. The knowledge generated by these...
Dissanayake, Sumudu N, Marinotti, Osvaldo, Ribeiro, Jose Marcos C, James, Anthony A
Background: The completed sequence of the Anopheles gambiae genome has enabled genome-wide analyses of gene expression and regulation in this principal vector of human malaria. These investigations...
Dissanayake, Sumudu N, Marinotti, Osvaldo, Ribeiro, Jose Marcos C, James, Anthony A
BACKGROUND: The completed sequence of the Anopheles gambiae genome has enabled genome-wide analyses of gene expression and regulation in this principal vector of human malaria. These investigations...
Marinotti, Osvaldo, NGUYEN, Quang K, Calvo, Eric, James, Anthony A, Ribeiro, Jose MC
A microarray analysis of 14 900 genes of the malaria vector mosquito, Anopheles gambiae, shows that as many as 33% (4924) of their corresponding transcription products vary in abundance within 24 h...
Calvo, Eric, Walter, Marika, Adelman, Zachary N., Jimenez, Alyssa, Onal, Sedef, Marinotti, Osvaldo, ...
A number of genetics-based strategies for the control of vector-borne diseases require the development of genetic drive systems for introgressing antipathogen effector genes into wild populations of...
The accumulation of specific mRNAs following multiple blood meals in Anopheles gambiae (2005)
Xavier, Nirmala, Marinotti, Osvaldo, James, Anthony A
One approach to genetic control of transmission of the parasites that cause human malaria is based on expressing effector genes in mosquitoes that disable the pathogens. Endogenous mosquito promoter...
Characterization of the c-type lysozyme gene family in Anopheles gambiae (2005)
Li, Bin, Calvo, Eric, Marinotti, Osvaldo, James, Anthony A., Paskewitz, Susan M.
Seven new c-type lysozyme genes were found using the Anopheles gambiae genome sequence, increasing to eight the total number of genes in this family identified in this species. The eight lysozymes in...
The AeAct-4 gene is expressed in the developing flight muscles of female Aedes aegypti (2004)
Muñoz, Delia, Jimenez, Alyssa J., Marinotti, Osvaldo, James, Anthony A.
Population reduction of mosquitoes is an effective method for controlling dengue fever and malaria transmission. Recent developments in control techniques include proposals to construct transgenic...
Romero, Lisa C, Nguyen, Thanh V, Deville, Benoit, Ogunjumo, Oluwasanmi, James, Anthony A
Abstract Background Identification and characterization of novel Plasmodium gene families is necessary for developing new anti-malarial therapeutics. The products of the Plasmodium falciparum gene,...
Jasinskiene, Nijole, Coates, Craig J., Ashikyan, Aurora, James, Anthony A.
The excision of specific DNA sequences from integrated transgenes in insects permits the dissection in situ of structural elements that may be important in controlling gene expression. Furthermore,...
Peng, Zhikang, Xu, Wenzhong, James, Anthony A., Lam, Herman, Sun, Dongfeng, Cheng, Liping, ...
Accurate diagnosis of mosquito allergy has been precluded by the difficulty of obtaining salivary allergens. In this study, we expressed, purified, characterized and investigated the clinical...
Robust gut-specific gene expression in transgenic Aedes aegypti mosquitoes. (2000)
Moreira, Luciano A., Edwards, Marten J., Adhami, Faisal, Jasinskiene, Nijole, James, Anthony A., Jacobs-Lorena, Marcelo
Genetic modification of the vectorial capacity of mosquito vectors of human disease requires promoters capable of driving gene expression with appropriate tissue and stage specificity. We report on...
FLP-mediated recombination in the vector mosquite, Aedes aegypti (1991)
Morris, Alison C., Schaub, Theresa L., James, Anthony A.
The activity of a yeast recombinase, FLP, on specific target DNA sequences, FRT, has been demonstrated in embryos of the vector mosquito, Aedes aegypti. In a series of experiments, plasmids...
Arcà, Bruno, Lombardo, Fabrizio, De Lara Capurro, Margareth, Della Torre, Alessandra, Dimopoulos, George, James, Anthony A., ...
The signal sequence trap method was used to isolate cDNAs corresponding to proteins containing secretory leader peptides and whose genes are expressed specifically in the salivary glands of the...
Engineering blood meal-activated systemic immunity in the yellow fever mosquito, Aedes aegypti
Kokoza, Vladimir, Ahmed, Abduelaziz, Cho, Wen-Long, Jasinskiene, Nijole, James, Anthony A., Raikhel, Alexander
Progress in molecular genetics makes possible the development of alternative disease control strategies that target the competence of mosquitoes to transmit pathogens. We tested the regulatory region...
Jasinskiene, Nijole, Coates, Craig J., Benedict, Mark Q., Cornel, Anthony J., Rafferty, Cristina Salazar, James, Anthony A., ...
The mosquito Aedes aegypti is the world’s most important vector of yellow fever and dengue viruses. Work is currently in progress to control the transmission of these viruses by genetically...
Mariner transposition and transformation of the yellow fever mosquito, Aedes aegypti
Coates, Craig J., Jasinskiene, Nijole, Miyashiro, Linda, James, Anthony A.
The mariner transposable element is capable of interplasmid transposition in the embryonic soma of the yellow fever mosquito, Aedes aegypti. To determine if this demonstrated mobility could be...
Johnson, Barbara W., Olson, Ken E., Allen-Miura, Tanya, Rayms-Keller, Alfredo, Carlson, Jonathan O., Coates, Craig J., ...
A rapid and reproducible method of inhibiting the expression of specific genes in mosquitoes should further our understanding of gene function and may lead to the identification of mosquito genes...
Robust gut-specific gene expression in transgenic Aedes aegypti mosquitoes
Moreira, Luciano A., Edwards, Marten J., Adhami, Faisal, Jasinskiene, Nijole, James, Anthony A., Jacobs-Lorena, Marcelo
Genetic modification of the vectorial capacity of mosquito vectors of human disease requires promoters capable of driving gene expression with appropriate tissue and stage specificity. We report on...
Genetics of Mosquito Vector Competence
Beerntsen, Brenda T., James, Anthony A., Christensen, Bruce M.
Mosquito-borne diseases are responsible for significant human morbidity and mortality throughout the world. Efforts to control mosquito-borne diseases have been impeded, in part, by the development...
Jasinskiene, Nijole, Coates, Craig J., Ashikyan, Aurora, James, Anthony A.
The excision of specific DNA sequences from integrated transgenes in insects permits the dissection in situ of structural elements that may be important in controlling gene expression. Furthermore,...
The MB2 gene family of Plasmodium species has a unique combination of S1 and GTP-binding domains
Romero, Lisa C, Nguyen, Thanh V, Deville, Benoit, Ogunjumo, Oluwasanmi, James, Anthony A
Franz, Alexander W. E., Sanchez-Vargas, Irma, Adelman, Zach N., Blair, Carol D., Beaty, Barry J., James, Anthony A., ...
Mosquitoes (Aedes aegypti) were genetically modified to exhibit impaired vector competence for dengue type 2 viruses (DENV-2). We exploited the natural antiviral RNA interference (RNAi) pathway in...
Arcà, Bruno, Lombardo, Fabrizio, De Lara Capurro, Margareth, Della Torre, Alessandra, Dimopoulos, George, James, Anthony A., ...
The signal sequence trap method was used to isolate cDNAs corresponding to proteins containing secretory leader peptides and whose genes are expressed specifically in the salivary glands of the...
Engineering blood meal-activated systemic immunity in the yellow fever mosquito, Aedes aegypti
Kokoza, Vladimir, Ahmed, Abduelaziz, Cho, Wen-Long, Jasinskiene, Nijole, James, Anthony A., Raikhel, Alexander
Progress in molecular genetics makes possible the development of alternative disease control strategies that target the competence of mosquitoes to transmit pathogens. We tested the regulatory region...
Jasinskiene, Nijole, Coates, Craig J., Benedict, Mark Q., Cornel, Anthony J., Rafferty, Cristina Salazar, James, Anthony A., ...
The mosquito Aedes aegypti is the world’s most important vector of yellow fever and dengue viruses. Work is currently in progress to control the transmission of these viruses by genetically...
Mariner transposition and transformation of the yellow fever mosquito, Aedes aegypti
Coates, Craig J., Jasinskiene, Nijole, Miyashiro, Linda, James, Anthony A.
The mariner transposable element is capable of interplasmid transposition in the embryonic soma of the yellow fever mosquito, Aedes aegypti. To determine if this demonstrated mobility could be...
Johnson, Barbara W., Olson, Ken E., Allen-Miura, Tanya, Rayms-Keller, Alfredo, Carlson, Jonathan O., Coates, Craig J., ...
A rapid and reproducible method of inhibiting the expression of specific genes in mosquitoes should further our understanding of gene function and may lead to the identification of mosquito genes...
Robust gut-specific gene expression in transgenic Aedes aegypti mosquitoes
Moreira, Luciano A., Edwards, Marten J., Adhami, Faisal, Jasinskiene, Nijole, James, Anthony A., Jacobs-Lorena, Marcelo
Genetic modification of the vectorial capacity of mosquito vectors of human disease requires promoters capable of driving gene expression with appropriate tissue and stage specificity. We report on...
Genetics of Mosquito Vector Competence
Beerntsen, Brenda T., James, Anthony A., Christensen, Bruce M.
Mosquito-borne diseases are responsible for significant human morbidity and mortality throughout the world. Efforts to control mosquito-borne diseases have been impeded, in part, by the development...
Jasinskiene, Nijole, Coates, Craig J., Ashikyan, Aurora, James, Anthony A.
The excision of specific DNA sequences from integrated transgenes in insects permits the dissection in situ of structural elements that may be important in controlling gene expression. Furthermore,...
The MB2 gene family of Plasmodium species has a unique combination of S1 and GTP-binding domains
Romero, Lisa C, Nguyen, Thanh V, Deville, Benoit, Ogunjumo, Oluwasanmi, James, Anthony A
Franz, Alexander W. E., Sanchez-Vargas, Irma, Adelman, Zach N., Blair, Carol D., Beaty, Barry J., James, Anthony A., ...
Mosquitoes (Aedes aegypti) were genetically modified to exhibit impaired vector competence for dengue type 2 viruses (DENV-2). We exploited the natural antiviral RNA interference (RNAi) pathway in...
Bridging the gaps in vector biology
Schneider, David S, James, Anthony A
Workshop on the Molecular and Population Biology of Mosquitoes and other Disease Vectors
Adelman, Zach N., Jasinskiene, Nijole, Onal, Sedef, Juhn, Jennifer, Ashikyan, Aurora, Salampessy, Michael, ...
Transposable elements (TEs) are proposed as a basis for developing drive systems to spread pathogen resistance genes through vector mosquito populations. The use of transcriptional and translational...
Preventing the Spread of Malaria and Dengue Fever Using Genetically Modified Mosquitoes
In this candid interview, Anthony A. James explains how mosquito genetics can be exploited to control malaria and dengue transmission. Population replacement strategy, the idea that transgenic...
Injection of An. stephensi Embryos to Generate Malaria-resistant Mosquitoes
Terenius, Olle, Juhn, Jennifer, James, Anthony A.
The introduction of exogenous genes into the genomes of mosquitoes requires microinjection techniques tailored to the specific species of interest. This video protocol demonstrates a method used by...
Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
Jasinskiene, Nijole, Juhn, Jennifer, James, Anthony A.
In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing...
Beaty, Barry J., Prager, Denis J., James, Anthony A., Jacobs-Lorena, Marcelo, Miller, Louis H., Law, John H., ...
Comparative genomics allows the discovery of cis-regulatory elements in mosquitoes
Sieglaff, Douglas H., Dunn, W. Augustine, Xie, Xiaohui S., Megy, Karyn, Marinotti, Osvaldo, James, Anthony A.
The discovery and mapping of cis-regulatory elements is important for understanding regulation of gene transcription in mosquito vectors of human diseases. Genome sequence data are available for 3...