Arnold J. M. Driessen

LmrCD is a major multidrug resistance transporter in Lactococcus lactis (2006)

The Sec machinery facilitates the translocation of proteins across and into biological membranes. In several of the Proteobacteria, this machinery contains accessory features that are not present in...

Lubelski, Jacek, Jong, Anne De, Merkerk, Ronald Van, Agustiandari, Herfita, Kuipers, Oscar P., Kok, Jan, ...

When Lactococcus lactis is challenged with drugs it displays a multidrug resistance (MDR) phenotype. In silico analysis of the genome of L. lactis indicates the presence of at least 40 putative MDR...

Sec-Mediated Transport of Posttranslationally Dehydrated Peptides in Lactococcus lactis (2006)

Kuipers, Anneke, Wierenga, Jenny, Rink, Rick, Kluskens, Leon D., Driessen, Arnold J.M., Kuipers, Oscar P., ...

Nisin is a lanthionine-containing antimicrobial peptide produced by Lactococcus lactis. Its (methyl)lanthionines are introduced by two posttranslational enzymatic steps involving the dehydratase...

YidC-mediated Membrane Insertion of Assembly Mutants of Subunit c of the F1F0 ATPase (2006)

Kol, Stefan, Turrell, Bryony R., Keyzer, Jeanine De, Laan, Martin Van Der, Nouwen, Nico, Driessen, Arnold J.M.

YidC is a member of the OxaI family of membrane proteins that has been implicated in the membrane insertion of inner membrane proteins in Escherichia coli. We have recently demonstrated that...

SecA Supports a Constant Rate of Preprotein Translocation (2006)

Tomkiewicz, Danuta, Nouwen, Nico, Leeuwen, Ruud Van, Tans, Sander, Driessen, Arnold J.M.

In Escherichia coli, secretory proteins (preproteins) are translocated across the cytoplasmic membrane by the Sec system composed of a protein-conducting channel, SecYEG, and an ATP-dependent motor...

Subunit a of Cytochrome o Oxidase Requires Both YidC and SecYEG for Membrane Insertion (2006)

Active-Site Residues in the Type IV Prepilin Peptidase Homologue PibD from the Archaeon Sulfolobus solfataricus (2006)

The Escherichia coli YidC protein belongs to the Oxa1 family of membrane proteins that facilitate the insertion of membrane proteins. Depletion of YidC in E. coli leads to a specific defect in the...

Szabó, Zalán, Albers, Sonja-Verena, Driessen, Arnold J.M.

Archaeal preflagellin peptidases and bacterial type IV prepilin peptidases belong to a family of aspartic acid proteases that cleave the leader peptides of precursor proteins with type IV prepilin...

Topologically Fixed SecG Is Fully Functional (2006)

Vries, Erhard Van Der, Berrelkamp, Greetje, Nouwen, Nico, Driessen, Arnold J.M.

It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY...

The Bifidobacterium longum NCIMB 702259T ctr Gene Codes for a Novel Cholate Transporter (2006)

Price, Claire E., Reid, Sharon J., Driessen, Arnold J.M., Abratt, Valerie R.

Preexposure of Bifidobacterium longum NCIMB 702259T to cholate caused increased resistance to cholate, chloramphenicol, and erythromycin. The B. longum ctr gene, encoding a cholate efflux...

Regulation of expression of the arabinose and glucose transporter genes in the thermophilic archaeon Sulfolobus solfataricus (2006)

Lubelska, Joanna M., Jonuscheit, Melanie, Schleper, Christa, Albers, Sonja-Verena, Driessen, Arnold J.M.

Sugar uptake in Sulfolobus solfataricus, a thermoacidophilic archaeon, occurs through high-affinity binding of protein-dependent ABC transporters. We have investigated the expression patterns of two...

Protein secretion in the Archaea: multiple paths towards a unique cell surface (2006)

Albers, Sonja-Verena, Szabó, Zalán, Driessen, Arnold J.M.

Archaea are similar to other prokaryotes in most aspects of cell structure but are unique with respect to the lipid composition of the cytoplasmic membrane and the structure of the cell surface....

Proton Motive Force-Dependent Hoechst 33342 Transport by the ABC Transporter LmrA of Lactococcus lactis (2005)

Lubelski, Jacek, Merkerk, Ronald Van, Mazurkiewicz, Piotr S., Driessen, Arnold J.M.

The fluorescent compound Hoechst 33342 is a substrate for many multidrug resistance (MDR) transporters and is widely used to characterize their transport activity. We have constructed mutants of the...

Post-translational Modification of Therapeutic Peptides By NisB, the Dehydratase of the Lantibiotic Nisin (2005)

Kluskens, Leon D., Kuipers, Anneke, Rink, Rick, Boef, Esther De, Fekken, Susan, Driessen, Arnold J.M., ...

Post-translationally introduced dehydroamino acids often play an important role in the activity and receptor specificity of biologically active peptides. In addition, a dehydroamino acid can be...

Lantibiotic Structures as Guidelines for the Design of Peptides That Can Be Modified by Lantibiotic Enzymes (2005)

Rink, Rick, Kuipers, Anneke, Boef, Esther De, Leenhouts, Kees J., Driessen, Arnold J.M., Moll, Gert N., ...

Lantibiotics are (methyl)lanthionine-containing bacterial peptides. (Methyl)lanthionines are posttranslationally introduced into the prepropeptides by biosynthetic enzymes that dehydrate serines and...

Conformational State of the SecYEG-Bound SecA Probed by Single Tryptophan Fluorescence Spectroscopy (2005)

Natale, Paolo, Blaauwen, Tanneke Den, Does, Chris Van Der, Driessen, Arnold J.M.

The SecYEG complex is a membrane-embedded channel that permits the passage of precursor proteins (preproteins) across the inner membrane of Escherichia coli. SecA is a molecular motor that associates...

Heterologously Expressed Bacterial and Human Multidrug Resistance Proteins Confer Cadmium Resistance to Escherichia coli (2005)

Achard-Joris, Maud, Driessen, Arnold J.M., Bourdineaud, Jean-Paul

The human MDR1 gene is induced by cadmium exposure although no resistance to this metal is observed in human cells overexpressing hMDR1. To access the role of MDR proteins in cadmium resistance,...

The Oligomeric Distribution of SecYEG is Altered by SecA and Translocation Ligands (2005)

Scheuring, Johannes, Braun, Nathalie, Nothdurft, Lars, Stumpf, Matthias, Veenendaal, Andreas K.J., Kol, Stefan, ...

The multimeric membrane protein complex translocase mediates the transport of preproteins across and integration of membrane proteins into the inner membrane of Escherichia coli. The translocase...

YidC – an evolutionary conserved device for the assembly of energy-transducing membrane protein complexes (2005)

Laan, Martin Van Der, Nouwen, Nico P., Driessen, Arnold J.M.

Members of the YidC/Oxa1/Alb3 membrane protein family are multifunctional mediators of membrane protein integration, folding and assembly into large complexes. Their evolutionary conserved and...

Covalently Dimerized SecA Is Functional in Protein Translocation (2005)

Keyzer, Jeanine De, Spelbrink, Robin E.J., Nijstad, Niels, Kruijff, Ben De, Nouwen, Nico, ...

The ATPase SecA provides the driving force for the transport of secretory proteins across the cytoplasmic membrane of Escherichia coli. SecA exists as a dimer in solution, but the exact oligomeric...

The Large First Periplasmic Loop of SecD and SecF Plays an Important Role in SecDF Functioning (2005)

Nouwen, Nico, Piwowarek, Magdalena, Berrelkamp, Greetje, Driessen, Arnold J.M.

A remarkable feature of proteins of the SecD and SecF family involved in protein translocation is that they possess a very large first periplasmic domain. Here we report that this large first...

Inactivation of Protein Translocation by Cold-Sensitive (2005)

Deletion of the yiaMNO transporter genes affects the growth characteristics of Escherichia coli K-12 (2005)

Most mutations in the yajC-secDF operon identified via genetic screens confer a cold-sensitive growth phenotype. Here we report that two of these mutations confer this cold-sensitive phenotype by...

Plantinga, Titia H., Does, Chris Van Der, Tomkiewicz, Danuta, Keulen, Geertje Van, Konings, Wil N., Driessen, Arnold J.M.

Binding-protein-dependent secondary transporters make up a unique transport protein family. They use a solute-binding protein in proton-motive-force-driven transport. Only a few systems have been...

Sec-Mediated Secretion of Bacteriocin Enterocin P by Lactococcus lactis (2005)

Herranz, Carmen, Driessen, Arnold J.M.

Most lactic acid bacterium bacteriocins utilize specific leader peptides and dedicated machineries for secretion. In contrast, the enterococcal bacteriocin enterocin P (EntP) contains a typical...

Analysis of ATPases of putative secretion operons in the thermoacidophilic archaeon Sulfolobus solfataricus (2005)

Albers, Sonja-Verena, Driessen, Arnold J.M.

Gram-negative bacteria use a wide variety of complex mechanisms to secrete proteins across their membranes or to assemble secreted proteins into surface structures. As most archaea only possess a...

Functional and structural characterization of the minimal Sec translocase of the hyperthermophile Thermotoga maritima (2005)

Pretz, Monika G., Remigy, Hervé, Swaving, Jelto, Albers, Sonja V., Garrido, Victoria G., Chami, Mohamed, ...

The genome of the hyperthermophilic bacterium Thermotoga maritima contains the genes that encode core subunits of the protein translocase, a complex consisting of the molecular motor SecA and the...

Two pores better than one? (2005)

PcMtr, an aromatic and neutral aliphatic amino acid permease of Penicillium chrysogenum (2004)

The movement of proteins through a cell’s membrane requires a dedicated molecular machine. A glimpse of this apparatus in action shows that it has two channels, and hints at how these pores might...

Trip, Hein, Evers, Melchior E., Driessen, Arnold J.M.

The gene encoding an aromatic and neutral aliphatic amino acid permease of Penicillium chrysogenum was cloned, functionally expressed and characterized in Saccharomyces cerevisiae M4276. The...

The protein-conducting channel SecYEG (2004)

Veenendaal, Andreas K.J., Does, Chris Van Der, Driessen, Arnold J.M.

In bacteria, the translocase mediates the translocation of proteins into or across the cytosolic membrane. It consists of a membrane embedded protein-conducting channel and a peripherally associated...

Energetics of wild-type and mutant multidrug resistance secondary transporter LmrP of Lactococcus lactis (2004)

Mazurkiewicz, Piotr, Driessen, Arnold J.M., Konings, Wil N.

LmrP, a proton/multidrug antiporter of Lactococcus lactis, transports a variety of cationic substrates. Previously, two membrane-embedded acidic residues, Asp142 and Glu327, have been reported to be...

Transporter’s evolution and carbohydrate metabolic clusters (2004)

Plantinga, Titia H., Does, Chris Van Der, Driessen, Arnold J.M.

The yiaQRS genes of Escherichia coli K-12 are involved in carbohydrate metabolism. Clustering of homologous genes was found throughout several unrelated bacteria. Strikingly, all four bacterial...

ydaG and ydbA of Lactococcus lactis Encode a Heterodimeric ATP-binding Cassette-type Multidrug Transporter (2004)

Lubelski, Jacek, Mazurkiewicz, Piotr, Merkerk, Ronald Van, Konings, Wil N., Driessen, Arnold J.M.

Multidrug resistance (MDR)-type transporters mediate the active extrusion of structurally and functionally dissimilar compounds from the cells, thereby rendering cells resistant to a range of drugs....

Uptake of the β-Lactam Precursor α-Aminoadipic Acid in Penicillium chrysogenum Is Mediated by the Acidic and the General Amino Acid Permease (2004)

Trip, Hein, Evers, Melchior E., Driessen, Arnold J.M.

External addition of the β-lactam precursor α-aminoadipic acid to the filamentous fungus Penicillium chrysogenum leads to an increased intracellular α-aminoadipic acid concentration and an...

NisT, the Transporter of the Lantibiotic Nisin, Can Transport Fully Modified, Dehydrated, and Unmodified Prenisin and Fusions of the Leader Peptide with Non-lantibiotic Peptides (2004)

Kuipers, Anneke, Boef, Esther De, Rink, Rick, Fekken, Susan, Kluskens, Leon D., Driessen, Arnold J.M., ...

Lantibiotics are lanthionine-containing peptide antibiotics. Nisin, encoded by nisA, is a pentacyclic lantibiotic produced by some Lactococcus lactis strains. Its thioether rings are...

F1F0 ATP synthase subunit c is a substrate of the novel YidC pathway for membrane protein biogenesis (2004)

Laan, Martin Van Der, Bechtluft, Philipp, Kol, Stef, Nouwen, Nico, Driessen, Arnold J.M.

The Escherichia coli YidC protein belongs to the Oxa1 family of membrane proteins that have been suggested to facilitate the insertion and assembly of membrane proteins either in cooperation with the...

Binding of SecA to the SecYEG Complex Accelerates the Rate of Nucleotide Exchange on SecA (2004)

Natale, Paolo, Swaving, Jelto, Does, Chris Van Der, Keyzer, Jeanine De, Driessen, Arnold J.M.

SecYEG translocase mediates the transport of preproteins across the inner membrane of Escherichia coli. SecA binds the membrane-embedded SecYEG protein-conducting channel with high affinity and then...

SecYEG Proteoliposomes Catalyze the Δψ-Dependent Membrane Insertion of FtsQ (2004)

Laan, Martin Van Der, Nouwen, Nico, Driessen, Arnold J.M.

In Escherichia coli, the insertion of most inner membrane proteins is mediated by the Sec translocase. Ribosome-bound nascent chains of Sec-dependent inner membrane proteins are targeted to the...

Facilitated Drug Influx by an Energy-uncoupled Secondary Multidrug Transporter (2004)

Mazurkiewicz, Piotr, Poelarends, Gerrit J., Driessen, Arnold J.M., Konings, Wil N.

The majority of bacterial multidrug resistance transporters belong to the class of secondary transporters. LmrP is a proton/drug antiporter of Lactococcus lactis that extrudes positively charged...

Functional characterization of the Escherichia coli K-12 yiaMNO transport protein genes (2004)

Plantinga, Titia H., Does, Chris Van Der, Badia, Josefa, Aguilar, Juan, Konings, Wil N., Driessen, Arnold J.M.

The yiaMNO genes of Escherichia coli K-12 encode a binding protein-dependent secondary, or tri-partite ATP-independent periplasmic (TRAP), transporter. Since only a few members of this family have...

Insights Into ABC Transport in Archaea (2004)

Albers, Sonja-Verena, Koning, Sonja M., Konings, Wil N., Driessen, Arnold J.M.

In archaea, ATP-binding cassette (ABC) transporters play a crucial role in substrate uptake, export, and osmoregulation. Archaeal substrate-binding-protein-dependent ABC transporters are equipped...

Crystal Structures of the ATPase Subunit of the Glucose ABC Transporter from Sulfolobus solfataricus: Nucleotide-free and Nucleotide-bound Conformations (2003)

Driessen, Arnold J.M., Dijkstra, Bauke W., Albers, Sonja V., Verdon, Grégory

The ABC-ATPase GlcV energizes a binding protein-dependent ABC transporter that mediates glucose uptake in Sulfolobus solfataricus. Here, we report high-resolution crystal structures of GlcV in...

Formation of the Productive ATP-Mg2+-bound Dimer of GlcV, an ABC-ATPase from Sulfolobus solfataricus (2003)

Driessen, Arnold J.M., Dijkstra, Bauke W., Oosterwijk, Niels Van, Albers, Sonja-Verena, Verdon, Grégory

The ABC-ATPase GlcV from Sulfolobus solfataricus energizes an ABC transporter mediating glucose uptake. In ABC transporters, two ABC-ATPases are believed to form a head-to-tail dimer, with both...

Crystal Structures of the ATPase Subunit of the Glucose ABC Transporter from Sulfolobus solfataricus: Nucleotide-free and Nucleotide-bound Conformations (2003)

Driessen, Arnold J.M., Dijkstra, Bauke W., Albers, Sonja V., Verdon, Grégory

The ABC-ATPase GlcV energizes a binding protein-dependent ABC transporter that mediates glucose uptake in Sulfolobus solfataricus. Here, we report high-resolution crystal structures of GlcV in...

Formation of the Productive ATP-Mg2+-bound Dimer of GlcV, an ABC-ATPase from Sulfolobus solfataricus (2003)

Driessen, Arnold J.M., Dijkstra, Bauke W., Oosterwijk, Niels Van, Albers, Sonja-Verena, Verdon, Grégory

The ABC-ATPase GlcV from Sulfolobus solfataricus energizes an ABC transporter mediating glucose uptake. In ABC transporters, two ABC-ATPases are believed to form a head-to-tail dimer, with both...

Direct Demonstration of ATP-dependent Release of SecA from a Translocating Preprotein by Surface Plasmon Resonance (2003)

Keyzer, Jeanine De, Does, Chris Van Der, Kloosterman, Tomas G., Driessen, Arnold J.M.

Translocase mediates the transport of preproteins across the inner membrane of Escherichia coli. SecA binds with high affinity to the membrane-embedded protein-conducting SecYEG complex and serves as...

Archaeal Homolog of Bacterial Type IV Prepilin Signal Peptidases with Broad Substrate Specificity (2003)

Albers, Sonja-Verena, Szabó, Zalán, Driessen, Arnold J.M.

A large number of secretory proteins in the thermoacidophile Sulfolobus solfataricus are synthesized as a precursor with an unusual leader peptide that resembles bacterial type IV prepilin signal...

Conditional Lethal Mutations Separate the M13 Procoat and Pf3 Coat Functions of YidC. Different YidC Structural Requirements for Membrane Protein Insertion (2003)

Chen, Minyong, Xie, Kun, Nouwen, Nico, Driessen, Arnold J.M., Dalbey, Ross E.

Conditional lethal YidC mutants have been isolated to decipher the role of YidC in the assembly of Sec-dependent and Sec-independent membrane proteins. We now show that the membrane insertion of the...

The Escherichia coli multidrug transporter MdfA catalyzes both electrogenic and electroneutral transport reactions (2003)

Lewinson, Oded, Adler, Julia, Poelarends, Gerrit J., Mazurkiewicz, Piotr, Driessen, Arnold J.M., Bibi, Eitan

The resistance of cells to many drugs simultaneously (multidrug resistance) often involves the expression of membrane transporters (Mdrs); each recognizes and expels a broad spectrum of chemically...

Purification and characterisation of a lactococcal aminoacylase (2003)

Curley, Peter, Does, Chris Van Der, Driessen, Arnold J.M., Kok, Jan, Sinderen, D. Van

The amd1-encoded aminoacylase from Lactococcus lactis MG1363 was cloned and overexpressed in Escherichia coli and purified. The assumed dimeric enzyme has a subunit molecular mass of about 42 kDa and...

Reconstitution of Purified Bacterial Preprotein Translocase in Liposomes (2003)

Does, Chris Van Der, Keyzer, Jeanine De, Laan, Martin Van Der, Driessen, Arnold J.M.

Crystal Structures of the ATPase Subunit of the Glucose ABC Transporter from Sulfolobus solfataricus: Nucleotide-free and Nucleotide-bound Conformations (2003)

Dijkstra, Bauke W., Albers, Sonja V., Driessen, Arnold J.M., Verdon, Grégory

The ABC-ATPase GlcV energizes a binding protein-dependent ABC transporter that mediates glucose uptake in Sulfolobus solfataricus. Here, we report high-resolution crystal structures of GlcV in...

Formation of the Productive ATP-Mg2+-bound Dimer of GlcV, an ABC-ATPase from Sulfolobus solfataricus (2003)

Driessen, Arnold J.M., Dijkstra, Bauke W., Oosterwijk, Niels Van, Albers, Sonja-Verena, Verdon, Grégory

The ABC-ATPase GlcV from Sulfolobus solfataricus energizes an ABC transporter mediating glucose uptake. In ABC transporters, two ABC-ATPases are believed to form a head-to-tail dimer, with both...

Purification, crystallization and preliminary X-ray diffraction analysis of an archaeal ABC-ATPase (2002)

Driessen, Arnold J.M., Dijkstra, Bauke W., Albers, Sonja-V., Verdon, Grégory

In the archaeon Sulfolobus solfataricus glucose uptake is mediated by an ABC transport system. The ABC-ATPase of this transporter (GlcV) has been overproduced in Escherichia coli and purified....

Purification, crystallization and preliminary X-ray diffraction analysis of an archaeal ABC-ATPase (2002)

Driessen, Arnold J.M., Dijkstra, Bauke W., Albers, Sonja-V., Verdon, Grégory

In the archaeon Sulfolobus solfataricus glucose uptake is mediated by an ABC transport system. The ABC-ATPase of this transporter (GlcV) has been overproduced in Escherichia coli and purified....

GTP Hydrolysis of Cell Division Protein FtsZ: Evidence that the Active Site Is Formed by the Association of Monomers (2002)

Scheffers, Dirk-Jan, Wit, Janny G. De, Blaauwen, Tanneke Den, Driessen, Arnold J.M.

The essential prokaryotic cell division protein FtsZ is a tubulin homologue that forms a ring at the division site. FtsZ forms polymers in a GTP-dependent manner. Recent biochemical evidence has...

δ-(L-α-Aminoadipyl)-L-cysteinyl-D-valine synthetase, that mediates the first committed step in penicillin biosynthesis, is a cytosolic enzyme (2002)

Kamp, Mart Van De, Berg, Marco Van Den, Sjollema, Klaas, Bovenberg, Roel A.L., Veenhuis, Marten, ...

Penicillin biosynthesis by Penicillium chrysogenum is a compartmentalized process. The first catalytic step is mediated by δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACV synthetase), a...

Cloning and characterization of an aromatic amino acid and leucine permease of Penicillium chrysogenum (2002)

Trip, Hein, Evers, Melchior E., Konings, Wil N., Driessen, Arnold J.M.

The gene encoding the amino acid permease ArlP (Aromatic and leucine Permease) was isolated from the filamentous fungus Penicillium chrysogenum after PCR using degenerated oligonucleotides based on...

SecY-SecY and SecY-SecG contacts revealed by site-specific crosslinking (2002)

Assessment of the microbody luminal pH in the filamentous fungus Penicillium chrysogenum (2002)

Protein translocation across the cytoplasmic membrane of Escherichia coli is mediated by the integral membrane complex SecYEG and the peripherally bound ATPase SecA. To probe the environment of the...

Breeuwer, Pieter, Abee, Tjakko, Konings, Wil N., Driessen, Arnold J.M.

The enzymes of the penicillin biosynthetic pathway in Penicillium chrysogenum are located in different subcellular compartments. Consequently, penicillin pathway precursors and the biologically...

Components required for membrane assembly of newly synthesized K+ channel KcsA (2002)

Dalen, Annemieke Van, Laan, Martin Van Der, Driessen, Arnold J.M., Killian, J. Antoinette, Kruijff, Ben De

An Escherichia coli in vitro transcription-translation system was used to study the components involved in the biogenesis of the homotetrameric potassium channel KcsA. We show that a functional...

The F286Y mutation of PrlA4 tempers the signal sequence suppressor phenotype by reducing the SecA binding affinity (2002)

Keyzer, Jeanine De, Does, Chris Van Der, Swaving, Jelto, Driessen, Arnold J.M.

SecYEG forms the protein-conducting channel of the Escherichia coli translocase. It binds the peripheral ATPase SecA that drives the preprotein translocation reaction. PrlA4 is a double mutant of...

Sugar transport in (hyper)thermophilic archaea (2002)

Koning, Sonja M., Albers, Sonja-Verena, Konings, Wil N., Driessen, Arnold J.M.

Hyperthermophilic archaea show important metabolic adaptations for growth on carbohydrates under hostile conditions. For carbohydrate uptake so far only ABC-type transporters have been described that...

SecDFyajC forms a heterotetrameric complex with YidC (2002)

Immediate GTP hydrolysis upon FtsZ polymerization (2002)

The Escherichia coli preprotein translocase is composed of a ‘preprotein conducting channel’ domain that consists of the peripherally bound translocation ATPase SecA and the heterotrimeric SecYEG...

Scheffers, Dirk-Jan, Driessen, Arnold J.M.

To understand the polymerization dynamics of FtsZ, a bacterial cell division protein similar to tubulin, insight is required into the nature of the nucleotide bound to the polymerized protein. In a...

Kinetic Analysis of the Translocation of Fluorescent Precursor Proteins into Escherichia coli Membrane Vesicles (2002)

Keyzer, Jeanine De, Does, Chris Van Der, Driessen, Arnold J.M.

Protein secretion in Escherichia coli is mediated by translocase, a multi-subunit membrane protein complex with SecA as ATP-driven motor protein and the SecYEG complex as translocation pore. A...

YidC and SecY Mediate Membrane Insertion of a Type I Transmembrane Domain (2002)

Houben, Edith N.G., Urbanus, Malene L., Laan, Martin Van Der, Driessen, Arnold J.M., Brunner, Josef, ...

YidC has been identified recently as an evolutionary conserved factor that is involved in the integration of inner membrane proteins (IMPs) in Escherichia coli. The discovery of YidC has inspired the...

The Core of the Bacterial Translocase Harbors a Tilted Transmembrane Segment 3 of SecE (2002)

Veenendaal, Andreas K.J., Does, Chris Van Der, Driessen, Arnold J.M.

The bacterial translocase mediates the translocation and membrane integration of proteins. The integral membrane proteins SecY and SecE are conserved core subunits of the translocase. Previous...

The cell membrane plays a crucial role in survival of bacteria and archaea in extreme environments (2002)

Konings, Wil N., Albers, Sonja-Verena, Koning, Sonja, Driessen, Arnold J.M.

The cytoplasmic membrane of bacteria and archaea determine to a large extent the composition of the cytoplasm. Since the ion and in particular the proton and/or the sodium ion electrochemical...

Signal peptides of secreted proteins of the archaeon Sulfolobus solfataricus: a genomic survey (2002)

Albers, Sonja-Verena, Driessen, Arnold J.M.

Analysis of the recently completed genome sequence of the thermoacidophilic archaeon Sulfolobus solfataricus reveals that about 4.2% of its proteome consists of putative secretory proteins with...

Purification, crystallization and preliminary X-ray diffraction analysis of an archaeal ABC-ATPase (2002)

Driessen, Arnold J.M., Dijkstra, Bauke W., Albers, Sonja-V., Verdon, Grégory

In the archaeon Sulfolobus solfataricus glucose uptake is mediated by an ABC transport system. The ABC-ATPase of this transporter (GlcV) has been overproduced in Escherichia coli and purified....

SecDFyajC is not required for the maintenance of the proton motive force (2001)

Nouwen, Nico, Laan, Martin Van Der, Driessen, Arnold J.M.

SecDFyajC of Escherichia coli is required for efficient export of proteins in vivo. However, the functional role of SecDFyajC in protein translocation is unclear. We evaluated the postulated function...

Substitution of a conserved aspartate allows cation-induced polymerization of FtsZ (2001)

Scheffers, Dirk-Jan, Wit, Janny G. De, Blaauwen, Tanneke Den, Driessen, Arnold J.M.

The prokaryotic tubulin homologue FtsZ polymerizes in vitro in a nucleotide dependent fashion. Here we report that replacement of the strictly conserved Asp212 residue of Escherichia coli FtsZ by a...

The polymerization mechanism of the bacterial cell division protein FtsZ (2001)

Scheffers, Dirk-Jan, Driessen, Arnold J.M.

Bacteria and archaea usually divide symmetrically by formation of a septum in the middle of the cell. A key event in cell division is the assembly of the FtsZ ring. FtsZ is the prokaryotic homolog of...

Translocation of proteins across the cell envelope of Gram-positive bacteria (2001)

Swaving, Jelto, Freudl, Roland, Driessen, Arnold J.M.

In contrast to Gram-negative bacteria, secretory proteins of Gram-positive bacteria only need to traverse a single membrane to enter the extracellular environment. For this reason, Gram-positive...

SecB, a molecular chaperone with two faces (2001)

Sugar transport in Sulfolobus solfataricus is mediated by two families of binding protein-dependent ABC transporters (2001)

SecB is a molecular chaperone unique to the phylum Proteobacteria, which includes the majority of known Gram-negative bacteria of medical, industrial and agricultural significance. SecB is involved...

Elferink, Marieke G.L., Albers, Sonja-V., Konings, Wil N., Driessen, Arnold J.M.

The extreme thermoacidophilic archaeon Sulfolobus solfataricus grows optimally at 80°C and pH 3 and uses a variety of sugars as sole carbon and energy source. Glucose transport in this organism is...

Cellobiose Uptake in the Hyperthermophilic Archaeon Pyrococcus furiosus Is Mediated by an Inducible, High-Affinity ABC Transporter (2001)

Koning, Sonja M., Elferink, Marieke G.L., Konings, Wil N., Driessen, Arnold J.M.

The hyperthermophilic archaeon Pyrococcus furiosus can utilize different β-glucosides, like cellobiose and laminarin. Cellobiose uptake occurs with high affinity (Km = 175 nM) and involves an...

Mapping the Sites of Interaction between SecY and SecE by Cysteine Scanning Mutagenesis (2001)

Veenendaal, Andreas K.J., Does, Chris Van Der, Driessen, Arnold J.M.

In Escherichia coli, the SecYEG complex mediates the translocation and membrane integration of proteins. Both genetic and biochemical data indicate interactions of several transmembrane segments...

Enterocin P Causes Potassium Ion Efflux from Enterococcus faecium T136 Cells (2001)

Herranz, Carmen, Cintas, Luis M., Hernández, Pablo E., Moll, Gert N., Driessen, Arnold J.M.

Enterocin P is a bacteriocin produced by Enterococcus faecium P13. We studied the mechanism of its bactericidal action using enterocin-P-sensitive E. faecium T136 cells. The bacteriocin is incapable...

A presequence- and voltage-sensitive channel of the mitochondrial preprotein translocase formed by Tim23 (2001)

Truscott, Kaye N., Kovermann, Peter, Geissler, Andreas, Merlin, Alessio, Meijer, Michiel, Driessen, Arnold J.M., ...

Proteins imported into the mitochondrial matrix are synthesized in the cytosol with an N-terminal presequence and are translocated through hetero-oligomeric translocase complexes of the outer and...

The structural basis of protein targeting and translocation in bacteria (2001)

Driessen, Arnold J.M., Manting, Erik H., Does, Chris Van Der

In Gram-negative bacteria, two distinct targeting routes assist in the proper localization of secreted and membrane proteins. Signal recognition particle (SRP) mainly targets ribosome-bound nascent...

Reconstitution of Sec-dependent membrane protein insertion: nascent FtsQ interacts with YidC in a SecYEG-dependent manner (2001)

Laan, Martin Van Der, Houben, Edith N.G., Nouwen, Nico, Luirink, Joen, Driessen, Arnold J.M.

The inner membrane protein YidC is associated with the preprotein translocase of Escherichia coli and contacts transmembrane segments of nascent inner membrane proteins during membrane insertion....

Bioenergetics and solute uptake under extreme conditions (2001)

Albers, Sonja-V., Driessen, Arnold J.M., Konings, Wil N.

The ion and particularly the proton and sodium ion permeabilities of cytoplasmic membranes play crucial roles in the bioenergetics of microorganisms. The proton and sodium permeabilities of membranes...

Comparison of the Membrane Interaction and Permeabilization by the Designed Peptide Ac-MB21-NH2 and Truncated Dermaseptin S3 (2000)

Moll, Gert N., Brul, Stanley, Konings, Wil N., Driessen, Arnold J.M.

Ac-MB21-NH2 (Ac-FASLLGKALKALAKQ-NH2) and dermaseptin S3(1-16)-NH2 (ALWKNMLKGIGKLAGK-NH2) are cationic amphipathic peptides with antimicrobial activity against a broad spectrum of microorganisms...

Diversity of transport mechanisms: common structural principles (2000)

Driessen, Arnold J.M., Rosen, Barry P., Konings, Wil N.

Traditionally, prokaryotic solute transport systems are classified into major groups based on the energetic requirement of the transport process. These include the secondary transporters that are...

The Uncoupling Efficiency and Affinity of Flavonoids for Vesicles (2000)

Recourt, Kees, Driessen, Arnold J.M., Dijk, Cees Van

The relative hydrophobicity and interaction of flavonoids with artificial membranes using vesicles was studied. At the same degree of hydroxylation, flavones were slightly more hydrophobic than...

Interaction of Bacillus subtilis CsaA with SecA and precursor proteins (2000)

Müller, Jörg P., Ozegowski, Jörg, Vettermann, Stefan, Swaving, Jelto, Driessen, Arnold J.M.

CsaA from the Gram-positive bacterium Bacillus subtilis has been identified previously as a suppressor of the growth and protein-export defect of Escherichia coli secA(Ts) mutants. CsaA has...

Escherichia coli translocase: the unravelling of a molecular machine (2000)

Manting, Erik H., Driessen, Arnold J.M.

Protein translocation across the bacterial cytoplasmic membrane has been studied extensively in Escherichia coli. The identification of the components involved and subsequent reconstitution of the...

Non-hydrolysable GTP-γ-S stabilizes the FtsZ polymer in a GDP-bound state (2000)

Scheffers, Dirk-Jan, Blaauwen, Tanneke Den, Driessen, Arnold J.M.

FtsZ, a tubulin homologue, forms a cytokinetic ring at the site of cell division in prokaryotes. The ring is thought to consist of polymers that assemble in a strictly GTP-dependent way. GTP, but not...

Sulfur Regulation of the Sulfate Transporter Genes sutA and sutB in Penicillium chrysogenum (2000)

Kamp, Mart Van De, Schuurs, Theo A., Vos, Arnold, Konings, Wil N., Driessen, Arnold J.M.

Penicillium chrysogenum uses sulfate as a source of sulfur for the biosynthesis of penicillin. Sulfate uptake and the mRNA levels of the sulfate transporter-encoding sutB and sutA genes are all...

The carboxyl terminus of the Bacillus subtilis SecA is dispensable for protein secretion and viability (2000)

Swaving, Jelto, Klein, Michael, Freudl, Roland, Driessen, Arnold J.M.

The Escherichia coli secretion-dedicated chaperone SecB targets a subset of proteins to the translocase by interacting with the carboxyl (C-) terminus of SecA. This region of SecA is highly conserved...

Non-bilayer Lipids Stimulate the Activity of the Reconstituted Bacterial Protein Translocase (2000)

Does, Chris Van Der, Swaving, Jelto, Klompenburg, Wim Van, Driessen, Arnold J.M.

To determine the phospholipid requirement of the preprotein translocase in vitro, the Escherichia coli SecYEG complex was purified in a delipidated form using the detergent dodecyl maltoside. SecYEG...

SecYEG assembles into a tetramer to form the active protein translocation channel (2000)

Manting, Erik H., Does, Chris Van Der, Remigy, Hervé, Engel, Andreas, Driessen, Arnold J.M.

Translocase mediates preprotein translocation across the Escherichia coli inner membrane. It consists of the SecYEG integral membrane protein complex and the peripheral ATPase SecA. Here we show by...

YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase (2000)

Scotti, Pier A., Urbanus, Malene L., Brunner, Josef, Heijne, Gunnar Von, Does, Chris Van Der, ...

In Escherichia coli, both secretory and inner membrane proteins initially are targeted to the core SecYEG inner membrane translocase. Previous work has also identified the peripherally associated...

Cysteine-Directed Cross-Linking Demonstrates That Helix 3 of SecE Is Close to Helix 2 of SecY and Helix 3 of a Neighboring SecE (1999)

Kaufmann, Andreas, Manting, Erik H., Veenendaal, Andreas K.J., Driessen, Arnold J.M., Does, Chris Van Der

Preprotein translocation in Escherichia coli is mediated by translocase, a multimeric membrane protein complex with SecA as the peripheral ATPase and SecYEG as the translocation pore. Unique...

Outer Membrane Protein A of Escherichia coli Inserts and Folds into Lipid Bilayers by a Concerted Mechanism (1999)

Kleinschmidt, Jörg H., Blaauwen, Tanneke Den, Driessen, Arnold J.M., Tamm, Lukas K.

Unfolded outer membrane protein A (OmpA) of Escherichia coli spontaneously inserts and refolds into lipid bilayers upon dilution of denaturing urea. In the accompanying paper, we have developed a new...

Zinc Stabilizes the SecB Binding Site of SecA (1999)

Fekkes, Peter, Wit, Janny G. De, Boorsma, André, Friesen, Robert H.E., Driessen, Arnold J.M.

The molecular chaperone SecB targets preproteins to SecA at the translocation sites in the cytoplasmic membrane of Escherichia coli. SecA recognizes SecB via its carboxyl-terminal 22 aminoacyl...

Thermodynamics of nucleotide binding to NBS-I of the Bacillus subtilis preprotein translocase subunit SecA (1999)

Blaauwen, Tanneke Den, Does, Chris Van Der, Driessen, Arnold J.M.

SecA is the dissociatable nucleotide and preprotein binding subunit of the bacterial translocase. The thermodynamics of nucleotide binding to soluble SecA at nucleotide binding site I were determined...

Homeostasis of the membrane proton permeability in Bacillus subtilis grown at different temperatures (1999)

Driessen, Arnold J.M., Costa, Milton S. Da, Konings, Wil N.

Bacillus subtilis was grown at its growth temperature limits and at various temperatures in between the lower and upper growth temperature boundary. Liposomes were made of the extracted membrane...

A unique short signal sequence in membrane-anchored proteins of Archaea (1999)

Albers, Sonja-Verena, Konings, Wil N., Driessen, Arnold J.M.

Sulfate Transport in Penicillium chrysogenum: Cloning and Characterization of the sutA and sutB Genes (1999)

Kamp, Mart Van De, Pizzinini, Enrica, Vos, Arnold, Schuurs, Theo A., Newbert, Roger W., ...

In industrial fermentations, Penicillium chrysogenum uses sulfate as the source of sulfur for the biosynthesis of penicillin. By a PCR-based approach, two genes, sutA and sutB, whose encoded products...

Substrate Specificity of the SecB Chaperone (1999)

Knoblauch, Nicola T.M., Rüdiger, Stefan, Schönfeld, Hans-Joachim, Driessen, Arnold J.M., Schneider-Mergener, Jens, Bukau, Bernd

The bacterial chaperone SecB assists translocation of proteins across the inner membrane. The mechanism by which it differentiates between secretory and cytosolic proteins is poorly understood. To...

Preprotein Translocation by a Hybrid Translocase Composed of Escherichia coli and Bacillus subtilis Subunits (1999)

Swaving, Jelto, Driessen, Arnold J.M.

Bacterial protein translocation is mediated by translocase, a multisubunit membrane protein complex that consists of a peripheral ATPase SecA and a preprotein-conducting channel with SecY, SecE, and...

SecA Is Not Required for Signal Recognition Particle-mediated Targeting and Initial Membrane Insertion of a Nascent Inner Membrane Protein (1999)

Scotti, Pier A., Valent, Quido A., Manting, Erik H., Urbanus, Malene L., Driessen, Arnold J.M., Oudega, Bauke, ...

In Escherichia coli, signal recognition particle (SRP)-dependent targeting of inner membrane proteins has been described. In vitro cross-linking studies have demonstrated that short nascent chains...

A Single Amino Acid Substitution in SecY Stabilizes the Interaction with SecA (1999)

Manting, Erik H., Kaufmann, Andreas, Does, Chris Van Der, Driessen, Arnold J.M.

The SecYEG complex constitutes a protein conducting channel across the bacterial cytoplasmic membrane. It binds the peripheral ATPase SecA to form the translocase. When isoleucine 278 in...

Complementary and Overlapping Selectivity of the Two-Peptide Bacteriocins Plantaricin EF and JK (1999)

Moll, Gert N., Akker, Emile Van Den, Hauge, Håvard H., Nissen-Meyer, Jon, Nes, Ingolf F., Konings, Wil N., ...

Plantaricin EF and JK are both two-peptide bacteriocins produced by Lactobacillus plantarum C11. The mechanism of plantaricin EF and JK action was studied on L. plantarum 965 cells. Both plantaricins...

Glucose Transport in the Extremely Thermoacidophilic Sulfolobus solfataricus Involves a High-Affinity Membrane-Integrated Binding Protein (1999)

Albers, Sonja-V., Elferink, Marieke G.L., Charlebois, Robert L., Sensen, Christoph W., Driessen, Arnold J.M., Konings, Wil N.

The archaeon Sulfolobus solfataricus grows optimally at 80°C and pH 2.5 to 3.5 on carbon sources such as yeast extracts, tryptone, and various sugars. Cells rapidly accumulate glucose. This...

Functional Identification of the Product of the Bacillus subtilis yvaL Gene as a SecG Homologue (1999)

Swaving, Jelto, Broekhuizen, Cees P., Rose, Matthias, Quax, Wim J., Driessen, Arnold J.M.

Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral...

Differential Dependence of Levansucrase and α-Amylase Secretion on SecA (Div) during the Exponential Phase of Growth of Bacillus subtilis (1999)

Leloup, Laurence, Driessen, Arnold J.M., Freudl, Roland, Chambert, Régis, Petit-Glatron, Marie-Françoise

SecA, the translocation ATPase of the preprotein translocase, accounts for 0.25% of the total protein in a degU32(Hy) Bacillus subtilis strain in logarithmic phase. The SecA level remained constant...

Protein Targeting to the Bacterial Cytoplasmic Membrane (1999)

Fekkes, Peter, Driessen, Arnold J.M.

Temporal Expression of the Bacillus subtilis secA Gene, Encoding a Central Component of the Preprotein Translocase (1999)

Herbort, Markus, Klein, Michael, Manting, Erik H., Driessen, Arnold J.M., Freudl, Roland

In Bacillus subtilis, the secretion of extracellular proteins strongly increases upon transition from exponential growth to the stationary growth phase. It is not known whether the amounts of some or...

Lipid membranes from halophilic and alkali-halophilic Archaea have a low H+ and Na+ permeability at high salt concentration (1999)

Driessen, Arnold J.M., Grant, William D., Konings, Wil N.

The influence of pH and the salt concentration on the proton and sodium ion permeability of liposomes formed from lipids of the halophile Halobacterium salinarum and the haloalkaliphile Halorubrum...

Bacteriocins: mechanism of membrane insertion and pore formation (1999)

Moll, Gert N., Konings, Wil N., Driessen, Arnold J.M.

Lactic acid bacteria produce several types of pore forming peptides. Class I bacteriocins are lantibiotics that contain (methyl)lanthionine residues that may form intramolecular thioether rings....

Compartmentalization and transport in β-lactam antibiotic biosynthesis by filamentous fungi (1999)

Kamp, Mart Van De, Driessen, Arnold J.M., Konings, Wil N.

A proper description of the biosynthesis of fungal β-lactam antibiotics requires detailed knowledge of the cell biology of the producing organisms. This involves a delineation of the...

Outer membrane protein A of Escherichia coli inserts and folds into lipid bilayersby a concerted mechanism (1999)

Kleinschmidt, Jörg H., Blaauwen, Tanneke Den, Driessen, Arnold J. M., Tamm, Lukas K.

Unfolded outer membrane protein A (OmpA) of Escherichia coli spontaneously inserts and refolds into lipid bilayers upon dilution of denaturing urea. In the accompanying paper, we have developed a new...

Plantaricin A Is an Amphiphilic α-Helical Bacteriocin-like Pheromone Which Exerts Antimicrobial and Pheromone Activities through Different Mechanisms (1998)

Hauge, Håvard Hildeng, Mantzilas, Dimitris, Moll, Gert N., Konings, Wil N., Driessen, Arnold J.M., Eijsink, Vincent G.H., ...

Production of bacteriocins by lactic acid bacteria is in some cases regulated by a quorum sensing mechanism that involves a secreted bacteriocin-like peptide pheromone. In the case of Lactobacillus...

Translocase-Bound SecA Is Largely Shielded from the Phospholipid Acyl Chains (1998)

Voorst, Frank Van, Does, Chris Van Der, Brunner, Josef, Driessen, Arnold J.M., Kruijff, Ben De

Protein translocation in Escherichia coli is mediated by the SecA ATPase bound to the SecYEG membrane protein complex. SecA translocation ATPase activity as well as protein translocation is dependent...

Interaction between SecA and SecYEG in Micellar Solution and Formation of the Membrane-Inserted State (1998)

Does, Chris Van Der, Manting, Erik H., Kaufmann, Andreas, Lutz, Marco, Driessen, Arnold J.M.

Preprotein translocation in Escherichia coli is mediated by the translocase with SecA as peripheral ATPase and SecY, SecE, and SecG as membrane domain. To facilitate large-scale purification of the...

The Sec system (1998)

Driessen, Arnold J.M., Fekkes, Peter

Proteins designated to be secreted by Escherichia coli are synthesized with an amino-terminal signal peptide and associate as nascent chains with the export-specific chaperone SecB. Translocation...

Biochemical characterization of the SecA protein of Streptomyces lividans. Interaction with nucleotides, binding to membrane vesicles and in vitro translocation of proAmy protein (1998)

Blanco, Jorge, Driessen, Arnold J.M., Coque, Juan José R., Martin, Juan F.

The SecA protein of Streptomyces lividans was purified to near electrophoretic homogeneity by means of FPLC from an overproducing strain harbouring plasmid pULA400, in which the secA gene was...

Translocation of the precursor of B-amylase into Bacillus subtilis membrane vesicles (1998)

Swaving, Jelto, Driessen, Arnold J.M.

Bacilli vigorously secrete proteins into the extracellular environment, and are therefore used in industry for the bulk production of enzymes such as proteinases and amylases. Studies on the...

Preprotein transfer to the Escherichia coli translocase requires the co-operative binding of SecB and the signal sequence to SecA (1998)

Fekkes, Peter, Wit, Janny G. De, Kimsey, Harvey H., Kumamoto, Carol A., Driessen, Arnold J.M.

In Escherichia coli, precursor proteins are targeted to the membrane-bound translocase by the cytosolic chaperone SecB. SecB binds to the extreme carboxy-terminus of the SecA ATPase translocase...

Mechanistic Properties of the Two-Component Bacteriocin Lactococcin G (1998)

Moll, Gert, Hildeng-Hauge, Håvard, Nissen-Meyer, Jon, Nes, Ingolf F., Konings, Wil N., Driessen, Arnold J.M.

Lactococcin G is a bacteriocin whose activity depends on the complementary action of two peptides, termed α and β. Biologically active, synthetic lactococcin G was used to study the mode of action...

The Lantibiotic Nisin Induces Transmembrane Movement of a Fluorescent Phospholipid (1998)

Moll, Gert N., Konings, Wil N., Driessen, Arnold J.M.

Nisin is a pore-forming antimicrobial peptide. The capacity of nisin to induce transmembrane movement of a fluorescent phospholipid in lipid vesicles was investigated. Unilamellar phospholipid...

PrlA4 prevents the rejection of signal sequence defective preproteins by stabilizing the SecA–SecY interaction during the initiation of translocation (1998)

Fekkes, Peter, Boorsma, Andre, Huie, Janet L., Silhavy, Thomas J., Driessen, Arnold J.M.

In Escherichia coli, precursor proteins are translocated across the cytoplasmic membrane by translocase. This multisubunit enzyme consists of a preprotein-binding and ATPase domain, SecA, and the...

Bioenergetics and cytoplasmic membrane stability of the extremely acidophilic, thermophilic archaeon Picrophilus oshimae (1998)

Driessen, Arnold J.M., Zillig, Wolfram, Konings, Wil N.

Picrophilus oshimae is an extremely acidophilic, thermophilic archae on that grows optimally at 60°C and at pH 0.7. It is an obligatory acidophile that does not grow at pH values above 4.0. The...

The essence of being extremophilic: the role of the unique archaeal membrane lipids (1998)

The positive inside rule is not determined by the polarity of Δψ (1998)

In extreme environments, mainly Archaea are encountered. The archaeal cytoplasmic membrane contains unique ether lipids that cannot easily be degraded, are temperature- and mechanically resistant,...

Albers, Sonja-Verena, Does, Chris Van Der, Driessen, Arnold J.M., Klompenburg, Wim Van

Mechanisms of multidrug transporters (1997)

Konings, Wil N., Driessen, Arnold J.M., Poolman, Bert, Veen, Hendrik W. Van, Bolhuis, Henk

Drug resistance, mediated by various mechanisms, plays a crucial role in the failure of the drug-based treatment of various infectious diseases. As a result, these infectious diseases re-emerge...

The Low-Affinity ATP Binding Site of the Escherichia coli SecA Dimer Is Localized at the Subunit Interface (1997)

Boorsma, Andre, Knoche, Maren, Schäfer, Hans-Jochen, Driessen, Arnold J.M.

The homodimeric SecA protein is the ATP-dependent force generator in the Escherichia coli precursor protein translocation cascade. SecA contains two essential nucleotide binding sites (NBSs), i.e.,...

Inhibition of Preprotein Translocation and Reversion of the Membrane Inserted State of SecA by a Carboxyl Terminus Binding MAb (1997)

Blaauwen, Tanneke Den, Wit, Janny G. De, Gosker, Harry, Does, Chris Van Der, Breukink, Eef-Jan, Leij, Lou De, ...

SecA is the peripheral subunit of the preprotein translocase of Escherichia coli. SecA consists of two independently folding domains, i.e., the N-domain bearing the high-affinity nucleotide binding...

Interaction of SecB with soluble SecA (1997)

Blaauwen, Tanneke Den, Terpetschnig, Ewald, Lakowicz, Joseph R., Driessen, Arnold J.M.

The preprotein binding molecular chaperone SecB functions by preventing the premature folding of the preprotein in the cytosol, and targeting it to the peripheral subunit SecA of the translocase at...

Slow fusion of liposomes composed of membrane-spanning lipids (1997)

Elferink, Marieke G.L., Breemen, Jan Van, Konings, Wil N., Driessen, Arnold J.M., Wilschut, Jan

The fusion characteristics of large unilamellar liposomes composed of bipolar tetraether lipids extracted from the thermophilic archaeon Sulfolobus acidocaldarius, was investigated. These lipids span...

A new family of prokaryotic transport proteins: binding protein-dependent secondary transporters (1997)

Driessen, Arnold J.M., Jacobs, Mariken H.J., Konings, Wil N.

In Vivo Cross-Linking of the SecA and SecY Subunits of the Escherichia coli Preprotein Translocase (1997)

Manting, Erik H., Does, Chris Van Der, Driessen, Arnold J.M.

Precursor protein translocation across the Escherichia coli inner membrane is mediated by the translocase, which is composed of a heterotrimeric integral membrane protein complex with SecY, SecE, and...

Role of Transmembrane pH Gradient and Membrane Binding in Nisin Pore Formation (1997)

Moll, Gert N., Clark, Jonathan, Chan, Weng C., Bycroft, Barrie W., Roberts, Gordon C.K., Konings, Wil N., ...

Nisin is a cationic antimicrobial peptide that belongs to the group of lantibiotics. It is thought to form oligomeric pores in the target membrane by a mechanism that requires the transmembrane...

The catalytic cycle of the Escherichia coli SecA ATPase comprises two distinct preprotein translocation events (1997)

Wit, Janny G. De, Driessen, Arnold J.M.

SecA is the ATP-dependent force generator in the Escherichia coli precursor protein translocation cascade, and is bound at the membrane surface to the integral membrane domain of the preprotein...

The molecular chaperone SecB is released from the carboxy-terminus of SecA during initiation of precursor protein translocation (1997)

Fekkes, Peter, Does, Chris Van Der, Driessen, Arnold J.M.

The chaperone SecB keeps precursor proteins in a translocation-competent state and targets them to SecA at the translocation sites in the cytoplasmic membrane of Escherichia coli. SecA is thought to...

Mechanisms of multidrug transporters (1997)

Konings, Wil N., Driessen, Arnold J.M., Poolman, Bert, Veen, Hendrik W. Van, Bolhuis, Henk

Drug resistance, mediated by various mechanisms, plays a crucial role in the failure of the drug-based treatment of various infectious diseases. As a result, these infectious diseases re-emerge...

Multidrug resistance in Lactococcus lactis: evidence for ATP-dependent drug extrusion from the inner leaflet of the cytoplasmic membrane (1996)

Konings, Wil N., Driessen, Arnold J.M., Poolman, Bert, Molenaar, Douwe, Veen, Hendrik W. Van, Bolhuis, Henk

Lactococcus lactis possesses an ATP-dependent drug extrusion system which shares functional properties with the mammalian multidrug resistance (MDR) transporter P-glycoprotein. One of the intriguing...

Energetics and Mechanism of Drug Transport Mediated by the Lactococcal Multidrug Transporter LmrP (1996)

Driessen, Arnold J.M., Poolman, Bert, Putman, Monique, Brands, Jan Roel, Veen, Hendrik W. Van, Bolhuis, Henk, ...

The gene encoding the secondary multidrug transporter LmrP of Lactococcus lactis was heterologously expressed in Escherichia coli. The energetics and mechanism of drug extrusion mediated by LmrP were...

Multidrug resistance mediated by a bacterial homolog of the human multidrug transporter MDR1 (1996)

Poolman, Bert, Kok, Jan, Oussenko, Irina, Bolhuis, Henk, Venema, Koen, Veen, Hendrik W. Van, ...

Resistance of Lactococcus lactis to cytotoxic compounds shares features with the multidrug resistance phenotype of mammalian tumor cells. Here, we report the gene cloning and functional...

Domain Interactions of the Peripheral Preprotein Translocase Subunit SecA (1996)

Blaauwen, Tanneke Den, Fekkes, Peter, Wit, Janny G. De, Kuiper, Wieny, Driessen, Arnold J.M.

The homodimeric SecA protein is the peripheral subunit of the preprotein translocase in bacteria. It binds the preprotein and promotes its translocation across the bacterial cytoplasmic membrane by...

Membrane composition and ion-permeability in extremophiles (1996)

Glutamate transport in Rhodobacter sphaeroides is mediated by a novel binding protein-dependent secondary transport system (1996)

Protons and sodium ions are the only used coupling ions in energy transduction in Bacteria and Archaea. At their growth temperature, the permeability of the cytoplasmic membrane of thermophilic...

Jacobs, Mariken H.J., Heide, Tiemen Van Der, Driessen, Arnold J.M., Konings, Wil N.

Growth of a glutamate transport-deficient mutant of Rhodobacter sphaeroides on glutamate as sole carbon and nitrogen source can be restored by the addition of millimolar amounts of Na+. Uptake of...

Bactericidal Mode of Action of Plantaricin C (1996)

González, Beatriz, Glaasker, Erwin, Kunji, Edmund R.S., Driessen, Arnold J.M., Suárez, Juan E., Konings, Wil N.

Plantaricin C is a bacteriocin produced by Lactobacillus plantarum LL441 that kills sensitive cells by acting on the cytoplasmic membrane. In contrast to its lack of impact on immune cells,...

Sulfate Transport in Penicillium chrysogenum Plasma Membranes (1996)

Hillenga, Dirk J., Versantvoort, Hanneke J.M., Driessen, Arnold J.M., Konings, Wil N.

Transport studies with Penicillium chrysogenum plasma membranes fused with cytochrome c oxidase liposomes demonstrate that sulfate uptake is driven by the transmembrane pH gradient and not by the...

Basic Amino Acid Transport in Plasma Membrane Vesicles of Penicillium chrysogenum (1996)

Hillenga, Dirk J., Versantvoort, Hanneke J.M., Driessen, Arnold J.M., Konings, Wil N.

The characteristics of the basic amino acid permease (system VI) of the filamentous fungus Penicillium chrysogenum were studied in plasma membranes fused with liposomes containing the beef heart...

Sodium-Coupled Energy Transduction in the Newly Isolated Thermoalkaliphilic Strain LBS3 (1996)

Prowe, Steffen G., Driessen, Arnold J.M., Antranikian, Garabed, Konings, Wil N.

Strain LBS3 is a novel anaerobic thermoalkaliphilic bacterium that grows optimally at pH 9.5 and 50°C. Since a high concentration of Na+ ions is required for growth, we have analyzed the primary...

Lactococcin G Is a Potassium Ion-Conducting, Two-Component Bacteriocin (1996)

Moll, Gert, Ubbink-Kok, Trees, Hildeng-Hauge, Håvard, Nissen-Meyer, Jon, Nes, Ingolf F., Konings, Wil N., ...

Lactococcin G is a novel lactococcal bacteriocin whose activity depends on the complementary action of two peptides, termed α and β. Peptide synthesis of the α and β peptides yielded biologically...

Mechanism of lantibiotic-induced pore-formation (1996)

Moll, Gert N., Roberts, Gordon C.K., Konings, Wil N., Driessen, Arnold J.M.

Nisin and other lantibiotics have a bacteriocidal effect against Gram-positive bacteria, and also inhibit the outgrowth of bacterial spores. The bacteriocidal effect appears to be due to the...

Sec-dependent preprotein translocation in bacteria (1996)

Blaauwen, Tanneke Den, Driessen, Arnold J.M.

Translocation of precursor proteins across the cytoplasmic membrane in bacteria is mediated by a multi-subunit protein complex termed translocase, which consists of the integral membrane heterotrimer...

SecA is an intrinsic subunit of the Escherichia coli preprotein translocase and exposes its carboxyl terminus to the periplasm (1996)

Does, Chris Van Der, Blaauwen, Tanneke Den, Wit, Janny G. De, Manting, Erik H., Groot, Nancy A., Fekkes, Peter, ...

SecA is the dissociable ATPase subunit of the Escherichia coli preprotein translocase, and cycles in a nucleotide-modulated manner between the cytosol and the membrane. Overproduction of the integral...

Multidrug resistance in Lactococcus lactis: evidence for ATP-dependent drug extrusion from the inner leaflet of the cytoplasmic membrane (1996)

Poolman, Bert, Molenaar, Douwe, Veen, Hendrik W. Van, Bolhuis, Henk, Driessen, Arnold J.M., Konings, Wil N.

Lactococcus lactis possesses an ATP-dependent drug extrusion system which shares functional properties with the mammalian multidrug resistance (MDR) transporter P-glycoprotein. One of the intriguing...

Energetics and Mechanism of Drug Transport Mediated by the Lactococcal Multidrug Transporter LmrP (1996)

Driessen, Arnold J.M., Poolman, Bert, Putman, Monique, Brands, Jan Roel, Veen, Hendrik W. Van, Bolhuis, Henk, ...

The gene encoding the secondary multidrug transporter LmrP of Lactococcus lactis was heterologously expressed in Escherichia coli. The energetics and mechanism of drug extrusion mediated by LmrP were...

Multidrug resistance mediated by a bacterial homolog of the human multidrug transporter MDR1 (1996)

Driessen, Arnold J.M., Poolman, Bert, Kok, Jan, Oussenko, Irina, Bolhuis, Henk, Venema, Koen, ...

Resistance of Lactococcus lactis to cytotoxic compounds shares features with the multidrug resistance phenotype of mammalian tumor cells. Here, we report the gene cloning and functional...

The Lactococcal lmrP Gene Encodes a Proton Motive Force-dependent Drug Transporter (1995)

Driessen, Arnold J.M., Poolman, Bert, Veen, Hendrik W. Van, Poelarends, Gerrit, Bolhuis, Henk, Konings, Wil N.

To genetically dissect the drug extrusion systems of Lactococcus lactis, a chromosomal DNA library was made in Escherichia coli and recombinant strains were selected for resistance to high...

Mechanistic Studies of Lantibiotic-Induced Permeabilization of Phospholipid Vesicles (1995)

Driessen, Arnold J.M., Kuiper, Wieny, Kamp, Mart Van De, Sahl, Hans-Georg, Konings, Ruud N.H., ...

Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes...

Thermostability of respiratory terminal oxidases in the lipid environment (1995)

Elferink, Marieke G.L., Bosmal, Tjibbe, Lolkema, Juke S., Gleiszner, Michael, Driessen, Arnold J.M., Konings, Wil N.

The effect of the lipid environment on the thermostability of three respiratory terminal oxidases was determined. Cytochrome-c oxidase from beef heart and Bacillus stearothermophilus were used as...

Mutacin II, a Bactericidal Lantibiotic from Streptococcus mutans (1995)

Chikindas, Michael L., Novák, Jan, Driessen, Arnold J.M., Konings, Wil N., Schilling, Kurt M., Caufield, Page W.

Mutacin II is a lantibiotic that is produced by group II Streptococcus mutans. It inhibits the growth of other streptococci as well as many other gram-positive microorganisms by a hitherto unknown...

Identification of the Magnesium-binding Domain of the High-affinity ATP-binding Site of the Bacillus subtilis and Escherichia coli SecA Protein (1995)

Klose, Michael, Wit, Janny G. De, Blaauwen, Tanneke Den, Freudl, Roland, Driessen, Arnold J.M.

The homodimeric SecA protein is the peripheral subunit of the translocase, and couples the hydrolysis of ATP to the translocation of precursor proteins across the bacterial cytoplasmic membrane. The...

Penicillium chrysogenum Takes up the Penicillin G Precursor Phenylacetic Acid by Passive Diffusion (1995)

Hillenga, Dirk J., Versantvoort, Hanneke J.M., Molen, Siep Van Der, Driessen, Arnold J.M., Konings, Wil N.

Penicillium chrysogenum utilizes phenylacetic acid as a side chain precursor in penicillin G biosynthesis. During industrial production of penicillin G, phenylacetic acid is fed in small amounts to...

Characterization of a Binding Protein-Dependent Glutamate Transport System of Rhodobacter sphaeroides (1995)

Jacobs, Mariken H.J., Driessen, Arnold J.M., Konings, Wil N.

The mechanism of L-glutamate uptake was studied in Rhodobacter sphaeroides. Uptake of L-glutamate is mediated by a high-affinity (Kt of 1.2 µM), shock-sensitive transport system that is inhibited by...

Expression of the gltP gene of Escherichia coli in a glutamate transport-deficient mutant of Rhodobacter sphaeroides restores chemotaxis to glutamate (1995)

Jacobs, Mariken H.J., Heide, Tiemen Van Der, Tolner, Berend, Driessen, Arnold J.M., Konings, Wil N.

Rhodobacter sphaeroides is chemotactic to glutamate and most other amino acids. In Escherichia coli, chemotaxis involves a membrane-bound sensor that either binds the amino acid directly or interacts...

Ion permeability of the cytoplasmic membrane limits the maximum growth temperature of bacteria and archaea (1995)

Ubbink-Kok, Trees, Elferink, Marieke G.L., Driessen, Arnold J.M., Konings, Wil N.

Protons and sodium ions are the most commonly used coupling ions in energy transduction in bacteria and archaea. At their growth temperature, the permeability of the cytoplasmic membrane of...

The Lactococcal lmrP Gene Encodes a Proton Motive Force-dependent Drug Transporter (1995)

Veen, Hendrik W. Van, Poelarends, Gerrit, Bolhuis, Henk, Poolman, Bert, Driessen, Arnold J.M., Konings, Wil N.

To genetically dissect the drug extrusion systems of Lactococcus lactis, a chromosomal DNA library was made in Escherichia coli and recombinant strains were selected for resistance to high...

Proton Motive Force-Driven and ATP-Dependent Drug Extrusion Systems in Multidrug-Resistant Lactococcus lactis (1994)

Poolman, Bert, Veen, Hendrik W. Van, Poelarends, Gerrit, Molenaar, Douwe, Bolhuis, Henk, Driessen, Arnold J.M., ...

Three mutants of Lactococcus lactis subsp. lactis MG1363, termed EthR, DauR, and RhoR, were selected for resistance to high concentrations of ethidium bromide, daunomycin, and rhodamine 6G,...

Stability and proton-permeability of liposomes composed of archaeal tetraether lipids (1994)

Elferink, Marieke G.L., Wit, Janny G. De, Driessen, Arnold J.M., Konings, Wil N.

Liposomes composed of tetraether lipids originating from the thermoacidophilic archaeon Sulfolobus acidocaldarius were analyzed for their stability and proton permeability from 20°C up to 80°C. At...

Bioenergetic aspects of the translocation of macromolecules across bacterial membranes (1994)

Palmen, Ronald, Driessen, Arnold J.M., Hellingwerf, Klaas J.

Bacteria are extremely versatile in the sense that they have gained the ability to transport all three major classes of biopolymers through their cell envelope: proteins, nucleic acids, and...

Structural and functional properties of plasma membranes from the filamentous fungus Penicillium chrysogenum (1994)

Hillenga, Dirk J., Versantvoort, Hanneke J.M., Driessen, Arnold J.M., Konings, Wil N.

Functional plasma membranes from the filamentous fungus Penicillium chrysogenum have been isolated with the objective of studying transport processes. The isolation procedure consists of three steps,...

Generation of proton-motive force by an archaeal terminal quinol oxidase from Sulfolobus acidocaldarius (1994)

Gleissner, Michael, Elferink, Marieke G.L., Driessen, Arnold J.M., Konings, Wil N., Anemüller, Stefan, Schäfer, Günter

The terminal quinol oxidase of the cytochrome aa3 type was isolated from the extreme thermo-acidophilic archaeon Sulfolobus acidocaldarius. In micellar solution, the enzyme oxidized various quinols...

NarK is a nitrite-extrusion system involved in anaerobic nitrate respiration by Escherichia coli (1994)

Rowe, John J., Ubbink-Kok, Trees, Molenaar, Douwe, Konings, Wil N., Driessen, Arnold J.M.

Escherichia coli can use nitrate as a terminal electron acceptor for anaerobic respiration. A polytopic membrane protein, termed NarK, has been implicated in nitrate uptake and nitrite excretion and...

Proton Motive Force-Driven and ATP-Dependent Drug Extrusion Systems in Multidrug-Resistant Lactococcus lactis (1994)

Veen, Hendrik W. Van, Poelarends, Gerrit, Molenaar, Douwe, Bolhuis, Henk, Poolman, Bert, Driessen, Arnold J.M., ...

Three mutants of Lactococcus lactis subsp. lactis MG1363, termed EthR, DauR, and RhoR, were selected for resistance to high concentrations of ethidium bromide, daunomycin, and rhodamine 6G,...

SecA, the Peripheral Subunit of the Escherichia coli Precursor Protein Translocase, Is Functional as a Dimer (1993)

Bacterial solute transport proteins in their lipid environment (1993)

SecA, the peripheral ATPase domain of the Escherichia coli precursor protein translocase, was denatured in 6 M guanidine hydrochloride. Circular dichroism and intrinsic tryptophan fluorescence...

Veld, Gerda In 't, Driessen, Arnold J.M., Konings, Wil N.

The cytoplasmic membrane of bacteria is a selective barrier that restricts entry and exit of solutes. Transport of solutes across this membrane is catalyzed by specific membrane proteins. Integral...

Energy-transducing properties of primary proton pumps reconstituted into archaeal bipolar lipid vesicles (1993)

Elferink, Marieke G.L., Wit, Janny G. De, Driessen, Arnold J.M., Konings, Wil N.

Archaeal lipids differ considerably from eubacterial and eukaryotic lipids in their structure and physical properties. From the membranes of the extreme thermophilic archaea Sulfolobus acidocaldarius...

In vitro pore-forming activity of the lantibiotic nisin. Role of protonmotive force and lipid composition (1993)

García Garcerá, Maria J., Elferink, Marieke G.L., Driessen, Arnold J.M., Konings, Wil N.

Nisin is a lantibiotic produced by some strains of Lactococcus luctis subsp. lactis. The target for nisin action is the cytoplasmic membrane of Gram-positive bacteria. Nisin dissipates the membrane...

Exchange, Efflux, and Substrate Binding by Cysteine Mutants of the Lactose Permease of Escherichia coli (1993)

Iwaarden, Pierre R. Van, Driessen, Arnold J.M., Lolkema, Juke S., Kaback, H. Ronald, Konings, Wil N.

In this study, wild-type lac permease and lac permease mutated at each of the eight cysteinyl residues in the molecule were solubilized from the membrane, purified, and reconstituted into...

Pediocin PA-1, a Bacteriocin from Pediococcus acidilactici PAC1.0, Forms Hydrophilic Pores in the Cytoplasmic Membrane of Target Cells (1993)

Chikindas, Michael L., García-Garcerá, Maria J., Driessen, Arnold J.M., Ledeboer, Aat M., Nissen-Meyer, Jon, Nes, Ingolf F., ...

Pediocin PA-1 is a bacteriocin which is produced by Pediococcus acidilactici PAC1.0. We demonstrate that pediocin PA-1 kills sensitive Pediococcus cells and acts on the cytoplasmic membrane. In...

Generation of a Proton Motive Force by Histidine Decarboxylation and Electrogenic Histidine/Histamine Antiport in Lactobacillus buchneri (1993)

Molenaar, Douwe, Bosscher, Jaap S., Brink, Bart Ten, Driessen, Arnold J.M., Konings, Wil N.

Lactobaciflus buchneri ST2A vigorously decarboxylates histidine to the biogenic amine histamine, which is excreted into the medium. Cells grown in the presence of histidine generate both a...

Characteristics and Osmoregulatory Roles of Uptake Systems for Proline and Glycine Betaine in Lactococcus lactis (1993)

Molenaar, Douwe, Hagting, Anja, Alkema, Harmen, Driessen, Arnold J.M., Konings, Wil N.

Lactococcus lactis subsp. lacti ML3 contains high pools of proline or betaine when grown under conditions of high osmotic strength. These pools are created by specific transport systems. A...

Characterization of the arcD Arginine:Ornithine Exchanger of Pseudomonas aeruginosa. Localization in the Cytoplasmic Membrane and a Topological Model (1993)

Bourdineaud, Jean-Paul, Heierli, Daniel, Gamper, Marianne, Verhoogt, Hans J.C., Driessen, Arnold J.M., Konings, Wil N., ...

The arcDABC operon of Pseudomonas aeruginosa encodes the enzymes of the arginine deiminase pathway and is induced by oxygen limitation. The arcD gene specifies a 53-kDa protein with...

Lysine 106 of the Putative Catalytic ATP-binding Site of the Bacillus subtilis SecA Protein Is Required for Functional Complementation of Escherichia coli secA Mutants in Vivo (1993)

Klose, Michael, Schimz, Karl-Ludwig, Wolk, Jeroen Van Der, Driessen, Arnold J.M., Freudl, Roland

The SecA protein is a major component of the cellular machinery that mediates the translocation of proteins across the Escherichia coli plasma membrane. The secA gene from Bacillus subtilis was...

Can the excretion of metabolites by bacteria be manipulated? (1992)

Driessen, Arnold J.M., Poolman, Bert, Konings, Wil N.

Bacteria can release metabolites into the environment by various mechanisms. Excretion may occur by passive diffusion or by the reversal of the uptake process when the internal concentration of the...

What we can learn from the effects of thiol reagents on transport proteins (1992)

Iwaarden, Pierre R. Van, Driessen, Arnold J.M., Konings, Wil N.

Many secondary membrane transport systems contain reactive sulfhydryl groups. In this review the applications of SH reagents for analyzing the role of sulfhydryl groups in membrane transport systems...

Bacterial protein translocation: kinetic and thermodynamic role of ATP and the protonmotive force (1992)

Acidic phospholipids are required during solubilization of amino acid transport systems of Lactococcus lactis (1992)

The energetic mechanism of preprotein export in Escherichia coli has been a source of controversy for many years. In vitro studies of translocation reactions that use purified soluble and membrane...

Veld, Gerda In 't, Vrije, Truus De, Driessen, Arnold J.M., Konings, Wil N.

The branched-chain amino acid transport system of Lactococcus lactis was solubilized with n-octyl β-D-glucopyranoside and reconstituted into proteoliposomes. Transport activity was recovered only...

Effect of the unsaturation of phospholipid acyl chains on leucine transport of Lactococcus lactis and membrane permeability (1992)

Veld, Gerda In 't, Driessen, Arnold J.M., Konings, Wil N.

The effect of the degree of unsaturation of the phospholipid acyl chains on the branched-chain amino acid transport system of Lactococcus lactis was investigated by the use of a membrane fusion...

The stability and functional properties of proteoliposomes mixed with dextran derivatives bearing hydrophobic anchor groups (1992)

Elferink, Marieke G.L., Wit, Janny G. De, Veld, Gerda In 't, Reichert, Anke, Driessen, Arnold J.M., Ringsdorf, Helmut, ...

Liposomes composed of Escherichia coli phospholipid were coated with polysaccharides bearing hydrophohic palmitoyl anchors. The effect on the stability of liposomes without or with integral membrane...

arcD, the First Gene of the arc Operon for Anaerobic Arginine Catabolism in Pseudomonas aeruginosa, Encodes an Arginine-Ornithine Exchanger (1992)

Verhoogt, Hans J.C., Smit, Hans, Abee, Tjakko, Gamper, Marianne, Driessen, Arnold J.M., Haas, Dieter, ...

In the absence of oxygen and nitrate, Pseudomonas aeruginosa metabolizes arginine via the arginine deiminase pathway, which allows slow growth on rich media. The conversion of arginine to ornithine,...

Functional Reconstitution of Membrane Proteins in Monolayer Liposomes from Bipolar Lipids of Sulfolobus acidocaldarius (1992)

Elferink, Marieke G.L., Wit, Janny G. De, Demel, Rudy, Driessen, Arnold J.M., Konings, Wil N.

Membranes of Sulfolobus acidocaldarius, an extreme thermophilic archaebacterium, are composed of unusual bipolar lipids. They consist of macrocyclic tetraethers with two polar heads linked by two...

Reconstitution of the Leucine Transport System of Lactococcus lactis into Liposomes Composed of Membrane-Spanning Lipids from Sulfolobus acidocaldarius (1992)

Veld, Gerda In ’t, Elferink, Marieke G.L., Driessen, Arnold J.M., Konings, Wil N.

The effect of bipolar tetraether lipids, extracted from the thermophilic archaebacterium Sulfolobus acidocaldarius, on the branched-chain amino acid transport system of the mesophilic bacterium...

Energy Transduction and Transport Processes in Thermophilic Bacteria (1992)

Konings, Wil N., Tolner, Berend, Speelmans, Gea, Elferink, Marieke G.L., Wit, Janny G. De, Driessen, Arnold J.M.

Bacterial growth at the extremes of temperature has remained a fascinating aspect in the study of membrane function and structure. The stability of the integral membrane proteins of thermophiles make...

Cloning and molecular characterization of the secY genes from Bacillus lichcniformis and Staphylococcus carnosus: comparative analysis of nine members of the SecY family (1992)

Tschauder, Silvia, Driessen, Arnold J.M., Freudl, Roland

SecY is a central component of the export machinery that mediates the translocation of secretory proteins across the plasma membrane of Escherichia coli. We have cloned and sequenced the secY genes...

Precursor protein translocation by the Escherichia coli translocase is directed by the protonmotive force (1992)

Can the excretion of metabolites by bacteria be manipulated? (1992)

The SecY/E protein of Escherichia coli was coreconstituted with the proton pump bacteriorhodopsin and cytochrome c oxidase yielding proteoliposomes capable of sustaining a protonmotive force (Δp) of...

Driessen, Arnold J.M., Poolman, Bert, Konings, Wil N.

Bacteria can release metabolites into the environment by various mechanisms. Excretion may occur by passive diffusion or by the reversal of the uptake process when the internal concentration of the...

Hydrophobic membrane thickness and lipid-protein interactions of the leucine transport system of Lactococcus lactis (1991)

Driessen, Arnold J.M., Veld, Gerda In 't, Konings, Wil N.

The effect of the phospholipid acyl chain carbon number on the activity of the branched-chain amino acid transport system of Lactococcus lactis has been investigated. Major fatty acids identified in...

ΔµH+ and ATP Function at Different Steps of the Catalytic Cycle of Preprotein Translocase (1991)

Schiebel, Elmar, Driessen, Arnold J.M., Hartl, Franz-Ulrich, Wickner, William

Preprotein translocation in E. coli requires ATP, the membrane electrochemical potential ΔµH+, and translocase, an enzyme with an ATPase domain (SecA) and the membrane-embedded SecY/E. Studies of...

The Enzymology of Protein Translocation across the Escherichia coli Plasma Membrane (1991)

Wickner, William, Driessen, Arnold J.M., Hartl, Franz-Ulrich

Converging physiological, genetic, and biochemical studies have established the salient features of preprotein translocation across the plasma membrane of Escherichia coli. Translocation is catalyzed...

Characterization of Purified, Reconstituted Site-directed Cysteine Mutants of the Lactose Permease of Escherichia coli (1991)

Iwaarden, Pierre R. Van, Driessen, Arnold J.M., Menick, Donald R., Kaback, H. Ronald, Konings, Wilhelmus N.

lac permease mutated at each of the 8 cysteinyl residues in the molecule was solubilized from the membrane, purified, and reconstituted into proteoliposomes. The transport activity of proteoliposomes...

Proton transfer is rate-limiting for translocation of precursor proteins by the Escherichia coli translocase (1991)

Driessen, Arnold J.M., Wickner, William

The protonmotive force stimulates translocation in vivo, in crude in vitro reactions, and in a purified, reconstituted reaction. Translocation activity is a function of the pH at the inner face of...

The Purified E. coli Integral Membrane Protein SecY/E Is Sufficient for Reconstitution of SecA-Dependent Precursor Protein Translocation (1990)

Brundage, Lorna, Hendrick, Joseph P., Schiebel, Elmar, Driessen, Arnold J.M., Wickner, William

We have previously reconstituted the soluble phase of precursor protein translocation in vitro using purified proteins (the precursor proOmpA, the chaperone SecB, and the ATPase SecA) in addition to...

Reactive exofacial sulfhydryl-groups on the arginine-ornithine antiporter of Lactococcus lactis (1990)

Nucleotide Sequence and Expression in Escherichia coli of the Lactococcus lactis Citrate Permease Gene (1990)

The effect of various sulfhydryl (SH)-specific reagents on arginine-ornithine antiport activity in membrane vesicles of Lactococcus lactis was studied. Little or no inhibition of arginine-ornithine...

David, Silke, Driessen, Arnold J.M., Simons, Guus, Vos, Willem M. De

The plasmid-encoded citrate determinant of the Lactococcus lactis subsp. lactis var. diacetylactis NCDO176 was cloned and functionally expressed in a Cit- Escherichia coli K-12 strain. From deletion...

Solubilization and functional reconstitution of the protein-translocation enzymes of Escherichia coli (1990)

Driessen, Arnold J.M., Wickner, William

The SecY protein and other membrane proteins of Escherchia coli were solubilized by mixed micelles of n-octyl β-D-glucopyranoside, phospholipids, and glycerol. Proteoliposomes formed from this...

Energy-Dependent Uptake of 4-Chlorobenzoate in the Coryneform Bacterium NTB-1 (1990)

Groenewegen, Peter E.J., Driessen, Arnold J.M., Konings, Wil N.

The uptake of 4-chlorobenzoate (4-CBA) in intact cells of the coryneform bacterium NTB-1 was investigated. Uptake and metabolism of 4-CBA were observed in cells grown in 4-CBA but not in...

ProOmpA contains secondary and tertiary structure prior to translocation and is shielded from aggregation by association with SecB protein (1990)

Lecker, Stewart H., Driessen, Arnold J.M., Wickner, William

Escherichia coli protein export involves cytosolic components termed molecular chaperones which function to stabilize precursors for membrane translocation. It has been suggested that chaperones...

Accumulation of a nod Gene Inducer, the Flavonoid Naringenin, in the Cytoplasmic Membrane of Rhizobium leguminosarum biovar viciae Is Caused by the pH-Dependent Hydrophobicity of Naringenin (1989)

Recourt, Kees, Driessen, Arnold J.M., Lugtenberg, Ben J.J.

Most Sym plasmid-localized nodulation genes of Rhizobium leguminosarum bv. viciae are only expressed upon activation of the NodD protein by plant flavonoids, e.g., naringenin. As part of a study on...

Kinetic Mechanism and Specificity of the Arginine-Ornithine Antiporter of Lactococcus lactis (1989)

Driessen, Arnold J.M., Molenaar, Douwe, Konings, Wil N.

The kinetic mechanism and specificity of the arginine-ornithine antiporter was investigated in membrane vesicles derived from Lactococcus lactis. Membrane vesicles loaded with ornithine, and diluted...

Transport of Basic Amino Acids by Membrane Vesicles of Lactococcus lactis (1989)

Driessen, Arnold J.M., Leeuwen, Cornelis Van, Konings, Wil N.

The uptake of the basic amino acids arginine, ornithine, and lysine was studied in membrane vesicles derived from cells of Lactococcus lactis which were fused with liposomes in which beef heart...

Transport of Amino Acids in Lactobacillus casei by Proton-Motive-Force-Dependent and Non-Proton-Motive-Force-Dependent Mechanisms (1989)

Strobel, Herbert J., Russell, James B., Driessen, Arnold J.M., Konings, Wil N.

Lactobacilus casei 393 cells which were energized with glucose (pH 6.0) took up glutamine, asparagine, glutamate, aspartate, leucine, and phenylalanine. Little or no uptake of several essential amino...

Mechanism and Energetics of Dipeptide Transport in Membrane Vesicles of Lactococcus lactis (1989)

Smid, Eddy J., Driessen, Arnold J.M., Konings, Wil N.

Alanyl-α-glutamate transport has been studied in Lactococcus lactis ML3 cells and in membrane vesicles fused with liposomes containing beefheart cytochrome c oxidase as a...

Secondary transport of amino acids by membrane vesicles derived from lactic acid bacteria (1989)

Amino Acid Transport by Membrane Vesicles of an Obligate Anaerobic Bacterium, Clostridium acetobutylicum (1988)

Lactococci are fastidious bacteria which require an external source of amino acids and many other nutrients. These compounds have to pass the membrane. However, detailed analysis of transport...

Driessen, Arnold J.M., Ubbink-Kok, Trees, Konings, Wil N.

Membrane vesicles were isolated from the obligate anaerobic bacterium Clostridium acetobutylicum. Beef heart mitochondrial cytochrome c oxidase was inserted in these membrane vesicles by membrane...

Transport of Diamines by Enterococcus faecalis Is Mediated by an Agmatine-Putrescine Antiporter (1988)

Driessen, Arnold J.M., Smid, Eddy J., Konings, Wil N.

Enterococcusfaecalis ATCC 11700 is able to use arginine and the diamine agmatine as a sole energy source. Via the highly homologous deiminase pathways, arginine and agmatine are converted into CO2,...

Sodium-Dependent Transport of Neutral Amino Acids by Whole Cells and Membrane Vesicles of Streptococcus bovis, a Ruminal Bacterium (1988)

Russell, James B., Strobel, Herbert J., Driessen, Arnold J.M., Konings, Wil N.

Streptococcus bovis JB1 cells were able to transport serine, threonine, or alanine, but only when they were incubated in sodium buffers. If glucose-energized cells were washed in potassium phosphate...

Effect of Cholesterol on the Branched-Chain Amino Acid Transport System of Streptococcus cremoris (1988)

Zheng, Tan, Driessen, Arnold J.M., Konings, Wil N.

The effect of cholesterol on the activity of the branched-chain amino acid transport system of Streptococcus cremoris was studied in membrane vesicles of S. cremoris fused with liposomes made of egg...

Light-Driven Amino Acid Uptake in Streptococcus cremoris or Clostridium acetobutylicum Membrane Vesicles Fused with Liposomes Containing Bacterial Reaction Centers (1988)

Crielaard, Wim, Driessen, Arnold J.M., Molenaar, Douwe, Hellingwerf, Klaas J., Konings, Wil N.

Reaction centers of the phototrophic bacterium Rhodopseudomonas palustris were introduced as proton motive force-generating systems in membrane vesicles of two anaerobic bacteria. Liposomes...

A synthetic peptide corresponding to the C-terminal 25 residues of phage MS2 coded lysis protein dissipates the protonmotive force in Escherichia coli membrane vesicles by generating hydrophilic pores (1988)

Goessens, Wil H.F., Driessen, Arnold J.M., Wilschut, Jan, Duin, Jan Van

The RNA phage MS2 encodes a protein, 75 amino acids long, that is necessary and sufficient for lysis of the host cell. DNA deletion analysis has shown that the lytic activity is confined to the...

Lipid Requirement of the Branched-Chain Amino Acid Transport System of Streptococcus cremoris (1988)

Driessen, Arnold J.M., Zheng, Tan, Veld, Gerda In ’t, Konings, Wil N.

The role of the membrane lipid composition on the transport protein of branched-chain amino acids of the homofermentative lactic acid bacterium Streptococcus cremoris has been investigated. The major...

Regulation of Arginine-Ornithine Exchange and the Arginine Deiminase Pathway in Streptococcus lactis (1987)

Konings, W.N., Driessen, Arnold J.M., Poolman, Bert

Streptococcus lactis metabolizes arginine by the argiqine deiminase (ADI) pathway. Resting cells of S. lactis grown in the presence of galactose and arginine maintain a high intracellular ornithine...

Regulation of Solute Transport in Streptococci by External and Internal pH Values (1987)

Konings, Wil N., Driessen, Arnold J.M., Poolman, Bert

Arginine transport in Streptococcus lactis is catalyzed by a cationic exchanger (1987)

Kiewiet, Rense, Poolman, Bert, Driessen, Arnold J.M., Konings, Wil N.

Streptococcus lactis metabolizes arginine via the arginine deiminase pathway to ornithine, CO2, NH3, and ATP. The translocation of arginine and ornithine has been studied using membrane vesicles of...

The effect of trypsin treatment on the incorporation and energy-transducing properties of bacteriorhodopsin in liposomes (1987)

Driessen, Arnold J.M., Hellingwerf, Klaas J., Konings, Wil N.

Bacteriorhodopsin and trypsin-modified bacteriorhodopsin have been reconstituted into liposomes by means of a low pH-sonication procedure. The incorporation of bacteriorhodopsin in these...

Protonmotive-force-driven leucine uptake in yeast plasma membrane vesicles (1987)

Opekarovà, Miroslava, Driessen, Arnold J.M., Konings, Wil N.

Plasma membranes prepared from Saccharomyces cerevisiae by the concanavalin A method were fused with liposomes by a freeze-thaw sonication method. A Δψ of -109 mV was generated in the sealed...

Neutral Amino Acid Transport by Membrane Vesicles of Streptococcus cremoris Is Subject to Regulation by Internal pH (1987)

Driessen, Arnold J.M., Kodde, Jan, Jong, Steven De, Konings, Wil N.

The pH dependence of transport of the neutral amino acids L-serine and L-alanine by membrane vesicles of Streptococcus cremoris have been studied in detail. The rates of four modes of facilitated...

Transport of Branched-Chain Amino Acids in Membrane Vesicles of Streptococcus cremoris (1987)

Driessen, Arnold J.M., Jong, Steven De, Konings, Wil N.

The kinetics, specificity, and mechanism of branched-chain amino acid transport in Streptococcus cremoris were studied in a membrane system of S. cremoris in which beef heart mitochondrial cytochrome...

Mechanism of Energy Coupling to Entry and Exit of Neutral and Branched Chain Amino Acids in Membrane Vesicles of Streptococcus cremoris (1987)

Driessen, Arnold J.M., Hellingwerf, Klaas J., Konings, Wil N.

The energetics of neutral and branched chain amino acid transport by membrane vesicles from Streptococcus cremoris have been studied with a novel model system in which beef heart mitochondrial...

Regulation of Arginine-Ornithine Exchange and the Arginine Deiminase Pathway in Streptococcus lactis (1987)

Driessen, Arnold J.M., Poolman, Bert, Konings, W.N.

Streptococcus lactis metabolizes arginine by the argiqine deiminase (ADI) pathway. Resting cells of S. lactis grown in the presence of galactose and arginine maintain a high intracellular ornithine...

Regulation of Solute Transport in Streptococci by External and Internal pH Values (1987)

Konings, Wil N., Driessen, Arnold J.M., Poolman, Bert

Arginine transport in Streptococcus lactis is catalyzed by a cationic exchanger (1987)

Poolman, Bert, Driessen, Arnold J.M., Kiewiet, Rense, Konings, Wil N.

Streptococcus lactis metabolizes arginine via the arginine deiminase pathway to ornithine, CO2, NH3, and ATP. The translocation of arginine and ornithine has been studied using membrane vesicles of...

Measurements of the proton motive force generated by cytochrome c oxidase from Bacillus subtilis in proteoliposomes and membrane vesicles (1986)

Vrij, Wim De, Driessen, Arnold J.M., Hellingwerf, Klaas J., Konings, Wil N.

Cytochrome c oxidase from Bacillus subtilis was reconstituted in liposomes and its energy-transducing properties were studied, The reconstitution procedure used included Ca2+-induced fusion of...

Functional incorporation of beef-heart cytochrome c oxidase into membranes of Streptococcus cremoris (1986)

Driessen, Arnold J.M., Vrij, Wim De, Konings, Wil N.

Beef heart mitochondrial cytochrome c oxidase has been incorporated into membrane vesicles derived from the homofermentative lactic acid bacterium Streptococcus cremoris. Proteoliposomes containing...

Calcium transport in membrane vesicles of Streptococcus cremoris (1986)