Arthur M. Friedlander

Anthrax Lethal Toxin Impairs Innate Immune Functions of Alveolar Macrophages and Facilitates Bacillus anthracis Survival (2006)

Ribot, Wilson J., Panchal, Rekha G., Brittingham, Katherine C., Ruthel, Gordon, Kenny, Tara A., Lane, Douglas, ...

Alveolar macrophages (AM) are very important for pulmonary innate immune responses against invading inhaled pathogens because they directly kill the organisms and initiate a cascade of innate and...

RECOMBINANT F1-V FUSION PROTEIN PROTECTS BLACK-FOOTED FERRETS (MUSTELA NIGRIPES) AGAINST VIRULENT YERSINIA PESTIS INFECTION (2004)

Tonie E. Rocke, Jordan Mencher, Susan R. Smith, Arthur M. Friedlander, Gerard P. Andrews, Laurie A. Baeten

Black-footed ferrets (Mustela nigripes) are highly susceptible to sylvatic plague, caused by the bacterium Yersinia pestis, and this disease has severely hampered efforts to restore ferrets to their...

Production and Characterization of Monoclonal Antibodies against the Lethal Factor Component of Bacillus anthracis Toxin (2003)

Little, Stephen F., Leppla, Stephen H., Friedlander, Arthur M.

The lethal toxin of Bacillus anthracis consists of two components, protective antigen and lethal factor. Protective antigen is cleaved after binding to cell receptors, yielding a receptor-bound...

Short-Course Postexposure Antibiotic Prophylaxis Combined with Vaccination Protects Against Experimental Inhalational Anthrax (1998)

Vietri, Nicholas J., Purcell, Bret K., Lawler, James V., Leffel, Elizabeth K., Rico, Pedro, Gamble, Christopher S., ...

Prevention of inhalational anthrax after Bacillus anthracis spore exposure requires a prolonged course of antibiotic prophylaxis. In response to the 2001 anthrax attack in the United States, ~10,000...

Vaccination against Anthrax with Attenuated Recombinant Strains of Bacillus anthracis That Produce Protective Antigen

Barnard, John P., Friedlander, Arthur M.

The protective efficacy of several live, recombinant anthrax vaccines given in a single-dose regimen was assessed with Hartley guinea pigs. These live vaccines were created by transforming ΔANR and...

Immune Response to Yersinia Outer Proteins and Other Yersinia pestis Antigens after Experimental Plague Infection in Mice

Benner, Gretchen E., Andrews, Gerard P., Byrne, W. Russell, Strachan, Susan D., Sample, Allen K., Heath, David G., ...

There is limited information concerning the nature and extent of the immune response to the virulence determinants of Yersinia pestis during the course of plague infection. In this study, we...

Macrophage-Derived Cell Lines Do Not Express Proinflammatory Cytokines after Exposure to Bacillus anthracis Lethal Toxin

Erwin, James L., DaSilva, Luis M., Bavari, Sina, Little, Stephen F., Friedlander, Arthur M., Chanh, Tran C.

We present evidence that Bacillus anthracis lethal toxin (LT) suppresses rather than induces proinflammatory cytokine production in macrophages. Suppression is observed with extremely low levels of...

Antibiotic Treatment of Experimental Pneumonic Plague in Mice

Byrne, William R., Welkos, Susan L., Pitt, M. Louise, Davis, Kelly J., Brueckner, Ralf P., Ezzell, John W., ...

A mouse model was developed to evaluate the efficacy of antibiotic treatment of pneumonic plague; streptomycin was compared to antibiotics with which there is little or no clinical experience....

Analysis of the Yersinia pestis V Protein for the Presence of Linear Antibody Epitopes

Pullen, Jeffrey K., Anderson, George W., Welkos, Susan L., Friedlander, Arthur M.

The V protein expressed by pathogenic Yersinia pestis is an important virulence factor and protective immunogen. The presence of linear B-cell epitopes in the V protein was investigated by using a...

Needle-Free Skin Patch Vaccination Method for Anthrax

Matyas, Gary R., Friedlander, Arthur M., Glenn, Gregory M., Little, Stephen, Yu, Jianmei, Alving, Carl R.

Three immunizations of mice with recombinant protective antigen (rPA) by transcutaneous immunization (TCI) induced long-term neutralizing antibody titers that were superior to those obtained with...

Enhancement of Anthrax Lethal Toxin Cytotoxicity: a Subset of Monoclonal Antibodies against Protective Antigen Increases Lethal Toxin-Mediated Killing of Murine Macrophages

Mohamed, Nehal, Li, Juan, Ferreira, Claudia S., Little, Stephen F., Friedlander, Arthur M., Spitalny, George L., ...

We investigated the ability of using monoclonal antibodies (MAbs) against anthrax protective antigen (PA), an anthrax exotoxin component, to modulate exotoxin cytotoxic activity on target macrophage...

Short-course postexposure antibiotic prophylaxis combined with vaccination protects against experimental inhalational anthrax

Vietri, Nicholas J., Purcell, Bret K., Lawler, James V., Leffel, Elizabeth K., Rico, Pedro, Gamble, Christopher S., ...

Prevention of inhalational anthrax after Bacillus anthracis spore exposure requires a prolonged course of antibiotic prophylaxis. In response to the 2001 anthrax attack in the United States,...

Vaccination against Anthrax with Attenuated Recombinant Strains of Bacillus anthracis That Produce Protective Antigen

Barnard, John P., Friedlander, Arthur M.

The protective efficacy of several live, recombinant anthrax vaccines given in a single-dose regimen was assessed with Hartley guinea pigs. These live vaccines were created by transforming ΔANR and...

Immune Response to Yersinia Outer Proteins and Other Yersinia pestis Antigens after Experimental Plague Infection in Mice

Benner, Gretchen E., Andrews, Gerard P., Byrne, W. Russell, Strachan, Susan D., Sample, Allen K., Heath, David G., ...

There is limited information concerning the nature and extent of the immune response to the virulence determinants of Yersinia pestis during the course of plague infection. In this study, we...

Macrophage-Derived Cell Lines Do Not Express Proinflammatory Cytokines after Exposure to Bacillus anthracis Lethal Toxin

Erwin, James L., DaSilva, Luis M., Bavari, Sina, Little, Stephen F., Friedlander, Arthur M., Chanh, Tran C.

We present evidence that Bacillus anthracis lethal toxin (LT) suppresses rather than induces proinflammatory cytokine production in macrophages. Suppression is observed with extremely low levels of...

Antibiotic Treatment of Experimental Pneumonic Plague in Mice

Byrne, William R., Welkos, Susan L., Pitt, M. Louise, Davis, Kelly J., Brueckner, Ralf P., Ezzell, John W., ...

A mouse model was developed to evaluate the efficacy of antibiotic treatment of pneumonic plague; streptomycin was compared to antibiotics with which there is little or no clinical experience....

Analysis of the Yersinia pestis V Protein for the Presence of Linear Antibody Epitopes

Pullen, Jeffrey K., Anderson, George W., Welkos, Susan L., Friedlander, Arthur M.

The V protein expressed by pathogenic Yersinia pestis is an important virulence factor and protective immunogen. The presence of linear B-cell epitopes in the V protein was investigated by using a...

Needle-Free Skin Patch Vaccination Method for Anthrax

Matyas, Gary R., Friedlander, Arthur M., Glenn, Gregory M., Little, Stephen, Yu, Jianmei, Alving, Carl R.

Three immunizations of mice with recombinant protective antigen (rPA) by transcutaneous immunization (TCI) induced long-term neutralizing antibody titers that were superior to those obtained with...

Enhancement of Anthrax Lethal Toxin Cytotoxicity: a Subset of Monoclonal Antibodies against Protective Antigen Increases Lethal Toxin-Mediated Killing of Murine Macrophages

Mohamed, Nehal, Li, Juan, Ferreira, Claudia S., Little, Stephen F., Friedlander, Arthur M., Spitalny, George L., ...

We investigated the ability of using monoclonal antibodies (MAbs) against anthrax protective antigen (PA), an anthrax exotoxin component, to modulate exotoxin cytotoxic activity on target macrophage...

Short-course postexposure antibiotic prophylaxis combined with vaccination protects against experimental inhalational anthrax

Vietri, Nicholas J., Purcell, Bret K., Lawler, James V., Leffel, Elizabeth K., Rico, Pedro, Gamble, Christopher S., ...

Prevention of inhalational anthrax after Bacillus anthracis spore exposure requires a prolonged course of antibiotic prophylaxis. In response to the 2001 anthrax attack in the United States,...

Anthrax Lethal Toxin Impairs Innate Immune Functions of Alveolar Macrophages and Facilitates Bacillus anthracis Survival

Ribot, Wilson J., Panchal, Rekha G., Brittingham, Katherine C., Ruthel, Gordon, Kenny, Tara A., Lane, Douglas, ...

Alveolar macrophages (AM) are very important for pulmonary innate immune responses against invading inhaled pathogens because they directly kill the organisms and initiate a cascade of innate and...

Poly-γ-Glutamate Capsule-Degrading Enzyme Treatment Enhances Phagocytosis and Killing of Encapsulated Bacillus anthracis▿

Scorpio, Angelo, Chabot, Donald J., Day, William A., O'Brien, David K., Vietri, Nicholas J., Itoh, Yoshifumi, ...

The poly-γ-d-glutamic acid capsule confers antiphagocytic properties on Bacillus anthracis and is essential for virulence. In this study, we showed that CapD, a γ-polyglutamic acid depolymerase...

Microarray Analysis of Transposon Insertion Mutations in Bacillus anthracis: Global Identification of Genes Required for Sporulation and Germination▿ †

Day, William A., Rasmussen, Suzanne L., Carpenter, Beth M., Peterson, Scott N., Friedlander, Arthur M.

A transposon site hybridization (TraSH) assay was developed for functional analysis of the Bacillus anthracis genome using a mini-Tn10 transposon which permitted analysis of 82% of this pathogen's...

Bacillus anthracis Spores of the bclA Mutant Exhibit Increased Adherence to Epithelial Cells, Fibroblasts, and Endothelial Cells but Not to Macrophages▿

Bozue, Joel, Moody, Krishna L., Cote, Christopher K., Stiles, Bradley G., Friedlander, Arthur M., Welkos, Susan L., ...

Bacillus anthracis is the causative agent of anthrax, and the spore form of the bacterium represents the infectious particle introduced into a host. The spore is surrounded by an exosporium, a...

Treatment of Experimental Anthrax with Recombinant Capsule Depolymerase▿

Scorpio, Angelo, Tobery, Steven A., Ribot, Wilson J., Friedlander, Arthur M.

Bacillus anthracis produces an antiphagocytic gamma-linked poly-d-glutamic acid capsule that is required for virulence. Capsule depolymerase (CapD) is a membrane-associated poly-γ-glutamate-specific...