Identification of a signature motif in target mRNAs of RNA-binding protein AUF1 (2009)
Mazan-Mamczarz, Krystyna, Kuwano, Yuki, Zhan, Ming, White, Elizabeth J., Martindale, Jennifer L., Lal, Ashish, ...
The ubiquitous RNA-binding protein AUF1 promotes the degradation of some target mRNAs, but increases the stability and translation of other targets. Here, we isolated AUF1-associated mRNAs by...
Nuclear HuR accumulation through phosphorylation by Cdk1 (2008)
Kim, Hyeon Ho, Abdelmohsen, Kotb, Lal, Ashish, Pullmann, Rudolf, Yang, Xiaoling, Galban, Stefanie, ...
A predominantly nuclear RNA-binding protein, HuR translocates to the cytoplasm in response to stress and proliferative signals, where it stabilizes or modulates the translation of target mRNAs. Here,...
Lal, Ashish, Warber, Sara, Kirakosyan, Ara, Kaufman, Peter B., Duke, James A.
Objective: In this study, our working hypothesis was that continuous light and fungal elicitation treatment of legume seedlings would lead to enhanced levels of isoflavonoids and soluble proteins....
A system for controlling executions of distributed protocols /-- by Ashish Lal. (1996)
Thesis (M.S.)--Kansas State University, 1996.
Identification of a target RNA motif for RNA-binding protein HuR
De Silanes, Isabel López, Zhan, Ming, Lal, Ashish, Yang, Xiaoling, Gorospe, Myriam
HuR, a protein that binds to specific mRNA subsets, is increasingly recognized as a pivotal posttranscriptional regulator of gene expression. Here, HuR was immunoprecipitated under conditions that...
Concurrent versus individual binding of HuR and AUF1 to common labile target mRNAs
Lal, Ashish, Mazan-Mamczarz, Krystyna, Kawai, Tomoko, Yang, Xiaoling, Martindale, Jennifer L, Gorospe, Myriam
RNA-binding proteins HuR and AUF1 bind to many common AU-rich target mRNAs and exert opposing influence on target mRNA stability, but the functional interactions between HuR and AUF1 have not been...
Antiapoptotic function of RNA-binding protein HuR effected through prothymosin α
Lal, Ashish, Kawai, Tomoko, Yang, Xiaoling, Mazan-Mamczarz, Krystyna, Gorospe, Myriam
We report the antiapoptotic effect of RNA-binding protein HuR, a critical regulator of the post-transcriptional fate of target transcripts. Among the most prominent mRNAs complexing with HuR is that...
Clean western blot signals from immunoprecipitated samples
Lal, Ashish, Haynes, Susan R., Gorospe, Myriam
We present a strategy that overcomes the high background arising during Western blotting (WB) detection of proteins obtained through immunoprecipitation (IP). Traditional HRP-conjugated secondary...
Translational Repression by RNA-Binding Protein TIAR†
Mazan-Mamczarz, Krystyna, Lal, Ashish, Martindale, Jennifer L., Kawai, Tomoko, Gorospe, Myriam
The RNA-binding protein TIAR has been proposed to inhibit protein synthesis transiently by promoting the formation of translationally silent stress granules. Here, we report the selective binding of...
Translational Control of Cytochrome c by RNA-Binding Proteins TIA-1 and HuR
Kawai, Tomoko, Lal, Ashish, Yang, Xiaoling, Galban, Stefanie, Mazan-Mamczarz, Krystyna, Gorospe, Myriam
Stresses affecting the endoplasmic reticulum (ER) globally modulate gene expression patterns by altering posttranscriptional processes such as translation. Here, we use tunicamycin (Tn) to...
Identification of a target RNA motif for RNA-binding protein HuR
De Silanes, Isabel López, Zhan, Ming, Lal, Ashish, Yang, Xiaoling, Gorospe, Myriam
HuR, a protein that binds to specific mRNA subsets, is increasingly recognized as a pivotal posttranscriptional regulator of gene expression. Here, HuR was immunoprecipitated under conditions that...
Concurrent versus individual binding of HuR and AUF1 to common labile target mRNAs
Lal, Ashish, Mazan-Mamczarz, Krystyna, Kawai, Tomoko, Yang, Xiaoling, Martindale, Jennifer L, Gorospe, Myriam
RNA-binding proteins HuR and AUF1 bind to many common AU-rich target mRNAs and exert opposing influence on target mRNA stability, but the functional interactions between HuR and AUF1 have not been...
Antiapoptotic function of RNA-binding protein HuR effected through prothymosin α
Lal, Ashish, Kawai, Tomoko, Yang, Xiaoling, Mazan-Mamczarz, Krystyna, Gorospe, Myriam
We report the antiapoptotic effect of RNA-binding protein HuR, a critical regulator of the post-transcriptional fate of target transcripts. Among the most prominent mRNAs complexing with HuR is that...
Translational Repression by RNA-Binding Protein TIAR†
Mazan-Mamczarz, Krystyna, Lal, Ashish, Martindale, Jennifer L., Kawai, Tomoko, Gorospe, Myriam
The RNA-binding protein TIAR has been proposed to inhibit protein synthesis transiently by promoting the formation of translationally silent stress granules. Here, we report the selective binding of...
Translational Control of Cytochrome c by RNA-Binding Proteins TIA-1 and HuR
Kawai, Tomoko, Lal, Ashish, Yang, Xiaoling, Galban, Stefanie, Mazan-Mamczarz, Krystyna, Gorospe, Myriam
Stresses affecting the endoplasmic reticulum (ER) globally modulate gene expression patterns by altering posttranscriptional processes such as translation. Here, we use tunicamycin (Tn) to...
Pullmann, Rudolf, Kim, Hyeon Ho, Abdelmohsen, Kotb, Lal, Ashish, Martindale, Jennifer L., Yang, Xiaoling, ...
RNA-binding proteins (RBPs) that associate with specific mRNA sequences and function as mRNA turnover and translation regulatory (TTR) RBPs are emerging as pivotal posttranscriptional regulators of...
p16INK4a Translation Suppressed by miR-24
Lal, Ashish, Kim, Hyeon Ho, Abdelmohsen, Kotb, Kuwano, Yuki, Pullmann, Rudolf, Srikantan, Subramanya, ...
RNA-Binding Proteins HuR and PTB Promote the Translation of Hypoxia-Inducible Factor 1α▿ †
Galbán, Stefanie, Kuwano, Yuki, Pullmann, Rudolf, Martindale, Jennifer L., Kim, Hyeon Ho, Lal, Ashish, ...
The levels of hypoxia-inducible factor 1α (HIF-1α) are tightly controlled. Here, we investigated the posttranscriptional regulation of HIF-1α expression in human cervical carcinoma HeLa cells...
Nuclear HuR accumulation through phosphorylation by Cdk1
Kim, Hyeon Ho, Abdelmohsen, Kotb, Lal, Ashish, Pullmann, Rudolf, Yang, Xiaoling, Galban, Stefanie, ...
A predominantly nuclear RNA-binding protein, HuR translocates to the cytoplasm in response to stress and proliferative signals, where it stabilizes or modulates the translation of target mRNAs. Here,...
Van Der Brug, Marcel P., Blackinton, Jeff, Chandran, Jayanth, Hao, Ling-Yang, Lal, Ashish, Mazan-Mamczarz, Krystyna, ...
Parkinson's disease (PD) is a major neurodegenerative condition with several rare Mendelian forms. Oxidative stress and mitochondrial function have been implicated in the pathogenesis of PD but the...
Identification of a signature motif in target mRNAs of RNA-binding protein AUF1
Mazan-Mamczarz, Krystyna, Kuwano, Yuki, Zhan, Ming, White, Elizabeth J., Martindale, Jennifer L., Lal, Ashish, ...
The ubiquitous RNA-binding protein AUF1 promotes the degradation of some target mRNAs, but increases the stability and translation of other targets. Here, we isolated AUF1-associated mRNAs by...