Quynh T. Phan, Carter L. Myers, Yue Fu, Donald C. Sheppard, Michael R. Yeaman, William H. Welch, ...
Candida albicans is the most common cause of hematogenously disseminated and oropharyngeal candidiasis. Both of these diseases are characterized by fungal invasion of host cells. Previously, we have...
Deferiprone iron chelation as a novel therapy for experimental mucormycosis (2006)
Ibrahim, Ashraf S., Edwards, John E., Fu, Yue, Spellberg, Brad
Objectives: Patients treated with the iron chelator deferoxamine are known to be more susceptible to mucormycosis. However, while deferoxamine is an iron chelator from the perspective of the human...
Comparison of antifungal treatments for murine fusariosis (2006)
Spellberg, Brad, Schwartz, Julie, Fu, Yue, Avanesian, Valentina, Adler-Moore, Jill, Edwards, John E., ...
Objectives: Fusarium solani infections are notoriously difficult to treat. We compared the efficacy of polyenes and an echinocandin in treating murine fusariosis to identify the optimal therapeutic...
Deferiprone iron chelation as a novel therapy for experimental mucormycosis (2006)
Ibrahim, Ashraf S., Edwards, John E., Fu, Yue, Spellberg, Brad
Objectives: Patients treated with the iron chelator deferoxamine are known to be more susceptible to mucormycosis. However, while deferoxamine is an iron chelator from the perspective of the human...
Comparison of antifungal treatments for murine fusariosis (2006)
Spellberg, Brad, Schwartz, Julie, Fu, Yue, Avanesian, Valentina, Adler-Moore, Jill, Edwards, John E., ...
Objectives: Fusarium solani infections are notoriously difficult to treat. We compared the efficacy of polyenes and an echinocandin in treating murine fusariosis to identify the optimal therapeutic...
Candida albicans RIM101 pH Response Pathway Is Required for Host-Pathogen Interactions
Davis, Dana, Edwards, John E., Mitchell, Aaron P., Ibrahim, Ashraf S.
The ability of Candida albicans to respond to diverse environments is critical for its success as a pathogen. The RIM101 pathway controls gene expression and the yeast-to-hyphal transition in C....
Mechanism of Fluconazole Resistance in Candida krusei
Orozco, Alison S., Higginbotham, Lindsey M., Hitchcock, Christopher A., Parkinson, Tanya, Falconer, Derek, Ibrahim, Ashraf S., ...
The mechanisms of fluconazole resistance in three clinical isolates of Candida krusei were investigated. Analysis of sterols of organisms grown in the absence and presence of fluconazole demonstrated...
Fu, Yue, Filler, Scott G., Spellberg, Bradley J., Fonzi, William, Ibrahim, Ashraf S., Kanbe, Toshio, ...
Adherence to the endothelial cell lining of the vasculature is probably a critical step in the egress of Candida albicans from the intravascular compartment. To identify potential adhesins that...
Secreted Aspartyl Proteinases and Interactions of Candida albicans with Human Endothelial Cells
Ibrahim, Ashraf S., Filler, Scott G., Sanglard, Dominique, Edwards, John E., Hube, Bernhard
The endothelial cell interactions of homozygous null mutants of Candida albicans that were deficient in secreted aspartyl proteinase 1 (Sap1), Sap2, or Sap3 were investigated. Only Sap2 was found to...
Unanticipated Heterogeneity in Growth Rate and Virulence among Candida albicans AAF1 Null Mutants
Rieg, Günter, Fu, Yue, Ibrahim, Ashraf S., Zhou, Xiang, Filler, Scott G., Edwards, John E.
The disruption of a specific gene in Candida albicans is commonly used to determine the function of the gene product. We disrupted AAF1, a gene of C. albicans that causes Saccharomyces cerevisiae to...
Spellberg, Brad, Johnston, Douglas, Phan, Quynh Trang, Edwards, John E., French, Samuel W., Ibrahim, Ashraf S., ...
We examined the relationship between host survival and renal and splenic immune responses in a murine model of hematogenously disseminated candidiasis. Male BALB/c mice were infected via tail vein...
Ibrahim, Ashraf S., Avanessian, Valentina, Spellberg, Brad, Edwards, John E.
The efficacies of liposomal amphotericin B (LAmB) and amphotericin B deoxycholate (AmB) were compared in a diabetic murine model of hematogenously disseminated Rhizopus oryzae infection. At 7.5 mg/kg...
Novel Inhalational Murine Model of Invasive Pulmonary Aspergillosis
Sheppard, Donald C., Rieg, Gunter, Chiang, Lisa Y., Filler, Scott G., Edwards, John E., Ibrahim, Ashraf S.
We developed a novel model of invasive aspergillosis (IA) that recapitulates human disease. Mice were immunosuppressed with cyclophosphamide and cortisone acetate and then infected in an aerosol...
VandenBerg, Alysia L., Ibrahim, Ashraf S., Edwards, John E., Toenjes, Kurt A., Johnson, Douglas I.
The yeast Candida albicans is a major opportunistic pathogen of immunocompromised individuals. It can grow in several distinct morphological states, including budded and hyphal forms, and the ability...
Ibrahim, Ashraf S., Spellberg, Brad J., Avenissian, Valentina, Fu, Yue, Filler, Scott G., Edwards, John E.
Candida spp. are opportunistic fungal pathogens that are among the most common causes of nosocomial bloodstream infections. The mortality attributable to disseminated candidiasis is 40 to 50% despite...
Rhizopus oryzae Adheres to, Is Phagocytosed by, and Damages Endothelial Cells In Vitro
Ibrahim, Ashraf S., Spellberg, Brad, Avanessian, Valentina, Fu, Yue, Edwards, John E.
Rhizopus oryzae is the most common cause of zygomycosis, a life-threatening infection that usually occurs in immunocompromised patients. A characteristic hallmark of zygomycosis is angioinvasion by...
Ibrahim, Ashraf S., Bowman, Joel C., Avanessian, Valentina, Brown, Keturah, Spellberg, Brad, Edwards, John E., ...
Rhizopus oryzae is the most common cause of zygomycosis, a life-threatening infection that usually occurs in patients with diabetic ketoacidosis. Despite standard therapy, the overall rate of...
Spellberg, Brad, Fu, Yue, Edwards, John E., Ibrahim, Ashraf S.
We studied the combination of amphotericin B lipid complex (ABLC) and caspofungin in mice with disseminated Rhizopus oryzae. Combination therapy improved survival compared to that of mice given...
Toenjes, Kurt A., Munsee, Suzanne M., Ibrahim, Ashraf S., Jeffrey, Rachel, Edwards, John E., Johnson, Douglas I.
The pathogenic yeast Candida albicans can exist in multiple morphological states, including budded, pseudohyphal, and true hyphal forms. The ability to convert between the budded and hyphal forms,...
Spellberg, Brad J., Ibrahim, Ashraf S., Avenissian, Valentina, Filler, Scott G., Myers, Carter L., Fu, Yue, ...
We have previously shown that intraperitoneal vaccination with the recombinant N terminus of Als1p (rAls1p-N) modestly improves survival during murine disseminated candidiasis. We now report marked...
Effects of Ploidy and Mating Type on Virulence of Candida albicans
Ibrahim, Ashraf S., Magee, B. B., Sheppard, D. C., Yang, Molly, Kauffman, Sarah, Becker, Jeff, ...
Candida albicans is the most common fungal pathogen of humans. The recent discovery of sexuality in this organism has led to the demonstration of a mating type locus which is usually heterozygous,...
Ibrahim, Ashraf S., Spellberg, Brad J., Avanesian, Valentina, Fu, Yue, Edwards, John E.
We have previously shown that vaccination with a vaccine based on the recombinant N-terminal domain of Als1p (rAls1p-N) protected BALB/c mice against disseminated infection caused by a single strain...
Antifungal Prophylaxis Is Effective against Murine Invasive Pulmonary Aspergillosis
Rieg, Gunter, Spellberg, Brad, Schwartz, Julie, Fu, Yue, Edwards, John E., Sheppard, Donald C., ...
Candida albicans RIM101 pH Response Pathway Is Required for Host-Pathogen Interactions
Davis, Dana, Edwards, John E., Mitchell, Aaron P., Ibrahim, Ashraf S.
The ability of Candida albicans to respond to diverse environments is critical for its success as a pathogen. The RIM101 pathway controls gene expression and the yeast-to-hyphal transition in C....
Mechanism of Fluconazole Resistance in Candida krusei
Orozco, Alison S., Higginbotham, Lindsey M., Hitchcock, Christopher A., Parkinson, Tanya, Falconer, Derek, Ibrahim, Ashraf S., ...
The mechanisms of fluconazole resistance in three clinical isolates of Candida krusei were investigated. Analysis of sterols of organisms grown in the absence and presence of fluconazole demonstrated...
Fu, Yue, Filler, Scott G., Spellberg, Bradley J., Fonzi, William, Ibrahim, Ashraf S., Kanbe, Toshio, ...
Adherence to the endothelial cell lining of the vasculature is probably a critical step in the egress of Candida albicans from the intravascular compartment. To identify potential adhesins that...
Secreted Aspartyl Proteinases and Interactions of Candida albicans with Human Endothelial Cells
Ibrahim, Ashraf S., Filler, Scott G., Sanglard, Dominique, Edwards, John E., Hube, Bernhard
The endothelial cell interactions of homozygous null mutants of Candida albicans that were deficient in secreted aspartyl proteinase 1 (Sap1), Sap2, or Sap3 were investigated. Only Sap2 was found to...
Unanticipated Heterogeneity in Growth Rate and Virulence among Candida albicans AAF1 Null Mutants
Rieg, Günter, Fu, Yue, Ibrahim, Ashraf S., Zhou, Xiang, Filler, Scott G., Edwards, John E.
The disruption of a specific gene in Candida albicans is commonly used to determine the function of the gene product. We disrupted AAF1, a gene of C. albicans that causes Saccharomyces cerevisiae to...
Spellberg, Brad, Johnston, Douglas, Phan, Quynh Trang, Edwards, John E., French, Samuel W., Ibrahim, Ashraf S., ...
We examined the relationship between host survival and renal and splenic immune responses in a murine model of hematogenously disseminated candidiasis. Male BALB/c mice were infected via tail vein...
Ibrahim, Ashraf S., Avanessian, Valentina, Spellberg, Brad, Edwards, John E.
The efficacies of liposomal amphotericin B (LAmB) and amphotericin B deoxycholate (AmB) were compared in a diabetic murine model of hematogenously disseminated Rhizopus oryzae infection. At 7.5 mg/kg...
Novel Inhalational Murine Model of Invasive Pulmonary Aspergillosis
Sheppard, Donald C., Rieg, Gunter, Chiang, Lisa Y., Filler, Scott G., Edwards, John E., Ibrahim, Ashraf S.
We developed a novel model of invasive aspergillosis (IA) that recapitulates human disease. Mice were immunosuppressed with cyclophosphamide and cortisone acetate and then infected in an aerosol...
VandenBerg, Alysia L., Ibrahim, Ashraf S., Edwards, John E., Toenjes, Kurt A., Johnson, Douglas I.
The yeast Candida albicans is a major opportunistic pathogen of immunocompromised individuals. It can grow in several distinct morphological states, including budded and hyphal forms, and the ability...
Ibrahim, Ashraf S., Spellberg, Brad J., Avenissian, Valentina, Fu, Yue, Filler, Scott G., Edwards, John E.
Candida spp. are opportunistic fungal pathogens that are among the most common causes of nosocomial bloodstream infections. The mortality attributable to disseminated candidiasis is 40 to 50% despite...
Rhizopus oryzae Adheres to, Is Phagocytosed by, and Damages Endothelial Cells In Vitro
Ibrahim, Ashraf S., Spellberg, Brad, Avanessian, Valentina, Fu, Yue, Edwards, John E.
Rhizopus oryzae is the most common cause of zygomycosis, a life-threatening infection that usually occurs in immunocompromised patients. A characteristic hallmark of zygomycosis is angioinvasion by...
Ibrahim, Ashraf S., Bowman, Joel C., Avanessian, Valentina, Brown, Keturah, Spellberg, Brad, Edwards, John E., ...
Rhizopus oryzae is the most common cause of zygomycosis, a life-threatening infection that usually occurs in patients with diabetic ketoacidosis. Despite standard therapy, the overall rate of...
Spellberg, Brad, Fu, Yue, Edwards, John E., Ibrahim, Ashraf S.
We studied the combination of amphotericin B lipid complex (ABLC) and caspofungin in mice with disseminated Rhizopus oryzae. Combination therapy improved survival compared to that of mice given...
Toenjes, Kurt A., Munsee, Suzanne M., Ibrahim, Ashraf S., Jeffrey, Rachel, Edwards, John E., Johnson, Douglas I.
The pathogenic yeast Candida albicans can exist in multiple morphological states, including budded, pseudohyphal, and true hyphal forms. The ability to convert between the budded and hyphal forms,...
Spellberg, Brad J., Ibrahim, Ashraf S., Avenissian, Valentina, Filler, Scott G., Myers, Carter L., Fu, Yue, ...
We have previously shown that intraperitoneal vaccination with the recombinant N terminus of Als1p (rAls1p-N) modestly improves survival during murine disseminated candidiasis. We now report marked...
Effects of Ploidy and Mating Type on Virulence of Candida albicans
Ibrahim, Ashraf S., Magee, B. B., Sheppard, D. C., Yang, Molly, Kauffman, Sarah, Becker, Jeff, ...
Candida albicans is the most common fungal pathogen of humans. The recent discovery of sexuality in this organism has led to the demonstration of a mating type locus which is usually heterozygous,...
Ibrahim, Ashraf S., Spellberg, Brad J., Avanesian, Valentina, Fu, Yue, Edwards, John E.
We have previously shown that vaccination with a vaccine based on the recombinant N-terminal domain of Als1p (rAls1p-N) protected BALB/c mice against disseminated infection caused by a single strain...
Antifungal Prophylaxis Is Effective against Murine Invasive Pulmonary Aspergillosis
Rieg, Gunter, Spellberg, Brad, Schwartz, Julie, Fu, Yue, Edwards, John E., Sheppard, Donald C., ...
Als3 Is a Candida albicans Invasin That Binds to Cadherins and Induces Endocytosis by Host Cells
Phan, Quynh T, Myers, Carter L, Fu, Yue, Sheppard, Donald C, Yeaman, Michael R, Welch, William H, ...
Candida albicans is the most common cause of hematogenously disseminated and oropharyngeal candidiasis. Both of these diseases are characterized by fungal invasion of host cells. Previously, we have...
The iron chelator deferasirox protects mice from mucormycosis through iron starvation
Ibrahim, Ashraf S., Gebermariam, Teclegiorgis, Fu, Yue, Husseiny, Mohamed I., French, Samuel W., ...
Mucormycosis causes mortality in at least 50% of cases despite current first-line therapies. Clinical and animal data indicate that the presence of elevated available serum iron predisposes the host...
Lin, Lin, Ibrahim, Ashraf S., Avanesian, Valentina, Edwards, John E., Fu, Yue, Baquir, Beverlie, ...
We are developing an anticandidal vaccine using the recombinant N terminus of Als3p (rAls3p-N). We report that although more rAls3p-N was bound by aluminum hydroxide diluted in saline than by...
Gene Overexpression/Suppression Analysis of Candidate Virulence Factors of Candida albicans▿
Fu, Yue, Luo, Guanpingsheng, Spellberg, Brad J., Edwards, John E., Ibrahim, Ashraf S.
We developed a conditional overexpression/suppression genetic strategy in Candida albicans to enable simultaneous testing of gain or loss of function in order to identify new virulence factors. The...
Comparison of Lipid Amphotericin B Preparations in Treating Murine Zygomycosis▿
Ibrahim, Ashraf S., Gebremariam, Teclegiorgis, Husseiny, Mohamed I., Stevens, David A., Fu, Yue, Edwards, John E., ...
We compared the efficacies of liposomal amphotericin B (LAmB) and an amphotericin B lipid complex (ABLC) in diabetic ketoacidotic (DKA) or neutropenic mice with disseminated zygomycosis. ABLC was as...
Combination Echinocandin-Polyene Treatment of Murine Mucormycosis▿
Ibrahim, Ashraf S., Gebremariam, Teclegiorgis, Fu, Yue, Edwards, John E., Spellberg, Brad
We previously found that caspofungin synergized with amphotericin B lipid complex in treating murine mucormycosis. We now report a similarly enhanced activity of liposomal amphotericin combined with...
Spellberg, Brad, Ibrahim, Ashraf S., Yeaman, Michael R., Lin, Lin, Fu, Yue, Avanesian, Valentina, ...
Vaccination with the recombinant N terminus of the candidal adhesin Als3p (rAls3p-N) protects mice from lethal candidemia. Candidal Als3p also is structurally similar to the microbial surface...
Genomic Analysis of the Basal Lineage Fungus Rhizopus oryzae Reveals a Whole-Genome Duplication
Ma, Li-Jun, Ibrahim, Ashraf S., Skory, Christopher, Grabherr, Manfred G., Burger, Gertraud, Butler, Margi, ...
Rhizopus oryzae is the primary cause of mucormycosis, an emerging, life-threatening infection characterized by rapid angioinvasive growth with an overall mortality rate that exceeds 50%. As a...
Posaconazole Mono- or Combination Therapy for Treatment of Murine Zygomycosis▿
Ibrahim, Ashraf S., Gebremariam, Teclegiorgis, Schwartz, Julie A., Edwards, John E., Spellberg, Brad
We compared the efficacy of combination posaconazole-liposomal amphotericin B (LAmB) therapy to monotherapy with either drug in diabetic ketoacidotic or neutropenic mice with disseminated zygomycosis...