ATSUSHI KOMAMINE, NATSUKO MURATA, KOJI NOMURA
Various aspects of somatic embryogenesis in carrot suspension cultures were reviewed on the basis of results obtained in our laboratory. We have established high-frequency and synchronous somatic...
Cell-Cycle Dependent Dynamic Change of 26S Proteasome Distribution in Tobacco BY-2 Cells (2002)
Yanagawa, Yuki, Hasezawa, Seiichiro, Kumagai, Fumi, Oka, Masayoshi, Fujimuro, Masahiro, Naito, Tae, ...
The 26S proteasome is known to play pivotal roles in cell-cycle progression in various eukaryotic cells; however, little is known about its role in higher plants. Here we report that the subcellular...
Yamamoto, Ken-ichi, Kobayashi, Naoko, Yoshitama, Kunijiro, Teramoto, Susumu, Komamine, Atsushi
Tyrosine hydroxylase was separated from polyphenol oxidase activity and was highly purified from betacyanin producing callus cultures of Portulaca grandiflora. The purified enzyme catalyzed the...
Shoji, Yuichi, Sugiyama, Munetaka, Komamine, Atsushi
The relationship between poly(ADP-ribose) synthesis and cytodifferentiation was studied in the well characterized Zinnia system, in which isolated mesophyll cells of Zinnia elegans transdifferentiate...
Shoji, Yuichi, Sugiyama, Munetaka, Komamine, Atsushi
5-Bromo-2′-deoxyuridine (BrdU), a thymidine analog, suppressed the transdifferentiation into tracheary elements (TE) of isolated mesophyll cells of Zinnia elegans without affecting cell division....
Suzuki, Kaoru, Ingold, Edgar, Sugiyama, Munetaka, Komamine, Atsushi
A paniculate fraction obtained from mesophyll cells of Zinnia elegans that were differentiating into tracheary elements exhibited xylan synthase activity, catalyzing the transfer of MC-xylose from...
Hirose, Masanori, Yamakawa, Takashi, Kodama, Tohru, Komamine, Atsushi
In suspension cultures of Vitis sp., maximal accumulation of anthocyanin was observed during the stationary phase. Accumulation of anthocyanin occurred in parallel with the cessation of cell division...
Sugiyama, Munetaka, Fukuda, Hiroo, Komamine, Atsushi
The inhibitory effect of 5-fluorodeoxyuridine (FdU) on the differentiation into tracheary elements was characterized in isolated mesophyll cells of Zinnia elegans. Both thymidine and uridine...
Suzuki, Kaoru, Amino, Shin-ichi, Takeuchi, Yuichi, Komamine, Atsushi
Differences in the composition of cell walls of two morphologically different lines (A and B) of suspension-cultured Catharanthus roseus cells, which have the same origin, were investigated. The...
Takeda, Junko, Senda, Mitsugi, Ozeki, Yoshihiro, Komamine, Atsushi
Membrane potentials of cultured carrot cells in culture medium were about −40 mV and did not change with addition of salts of addition (or depletion) of 2,4-dichlorophenoxyacetic acid (2,4-D). When...
Ingold, Edgar, Sugiyama, Munetaka, Komamine, Atsushi
Changes in the cell walls and their sugar composition during the formation of tracheary elements (TE) were analyzed using a culture of single cells isolated from the mesophyll of Zinnia elegans. By...
Sugiyama, Munetaka, Komamine, Atsushi
The effect of m-aminobenzamide (m-ABm) and nicotinamide, inhibitors of ADP-ribosyltransferase (ADP-RT), on the differentiation of tracheary elements was investigated using isolated mesophyll cells of...
Sugiyama, Munetaka, Fukuda, Hiroo, Komamine, Atsushi
The effects of nutrient limitation and γ-irradiation on tracheary element differentiation and cell division were investigated using single cells isolated from the mesophyll of Zinnia elegans. When...
Ozeki, Yoshihiro, Komamine, Atsushi
The effects of plant growth regulators were investigated on anthocyanin synthesis induced by removing auxin from carrot suspension cultures. Of the auxins tested, 2,4-D showed the strongest...
Ozeki, Yoshihiro, Komamine, Atsushi
The activities of enzymes involved in general phenylpropanoid metabolism were followed in a carrot suspension culture during the induction and reduction of anthocyanin synthesis regulated by 2,4-D....
Amino, Shin-ichi, Komamine, Atsushi
Pulse-chase experiments were done using a synchronous culture of Catharanthus roseus in order to study cell wall turnover during the cell cycle. [14C]Glucose was fed for 1 h to cells 35 and 49 h...
Masuda, Hiroshi, Ozeki, Yoshihiro, Amino, Shin-ichi, Komamine, Atsushi
Changes in the activities of some glycosidases were studied in carrot suspension cultures with and without 2,4-D. Remarkable cell elongation occurs in a medium without 2,4-D, while few cells elongate...
OZEKI, Yoshihiro, SAKANO, Katsuhiro, KOMAMINE, Atsushi, TANAKA, Yoshiyuki, NOGUCHI, Hiroshi, SANKAWA, Ushio, ...
Chalcone synthase was purified to homogeneity by polyacrylamide gel electrophoresis from cell suspension cultures of carrot in which anthocyanin synthesis was induced by transferring the cells from a...
Fukuda, Hiroo, Komamine, Atsushi
Cycloheximide (CH) prevented tracheary element (TE) differentiation and cell division in a culture of single cells isolated from the mesophyll of Zinnia elegans at the concentrations which inhibited...
Takeuchi, Yuichi, Komamine, Atsushi
Protoplasts prepared from suspension-cultured Vinca rosea cells were grown in a liquid medium, and the effects of osmolarity and growth regulators on cell division and on the composition of...
Mechanism of Suppression of DOPA Accumulation in a Callus Culture of Stizolobium hassjoo (1981)
Obata-Sasamoto, Hamako, Nishi, Noriko, Komamine, Atsushi
Mechanisms of suppression of 3,4-dihydroxyphenylalanine (DOPA) accumulation were investigated in a callus culture of Stizolobium hassjoo. DOPA was detected in the callus but in a much smaller amount...
Fukuda, Hiroo, Komamine, Atsushi
Effects of inhibitors of DNA synthesis on tracheary element (TE) differentiation were investigated in a culture of single cells isolated from the mesophyll of Zinnia elegans L. cv. Canary bird. In...
KANAMORI-FUKUDA, IKUKO, ASHIHARA, HIROSHI, KOMAMINE, ATSUSHI
Marked changes in the activity of the ‘de novo’ and ‘salvage’ pathways of pyrimidine biosynthesis during growth of Vinca rosea cells in a batch suspension culture were observed. The activity...
Glucans in the Cell Walls Regenerated from Vinca rosea Protoplasts (1981)
Takeuchi, Yuichi, Komamine, Atsushi
Protoplasts prepared from suspension-cultured Vinca rosea cells were cultured for 5 days. The cell walls regenerated from the protoplasts were mainly composed of glucans having 1,3- and 1,4-linkages....
Komamine, Atsushi, Shimizu, Tomiko, Ashihara, Hiroshi, Shimokoriyama, Masami
The rates of both O2 uptake in air and CO2 output in N2 per protein nitrogen and per cell increased markedly during the lag phase of callus formation, and decreased rapidly then gradually during the...
Vessel element formation in cultured carrot-root phloem slices (1971)
MIZUNO, KÔICHI, KOMAMINE, ATSUSHI, SHIMOKORIYAMA, MASAMI
The effects of light, auxin and cytokinin on vessel element formation in phloem slices of carrot root were examined. When slices of carrot cultivars, ‘Nakamura-senko-futo’ and...
RESPIRATORY METABOLISM IN THE PHLOEM, XYLEM AND CAMBIUM OF CARROT ROOT (1967)
MOROHASHI, YUKIO, KOMAMINE, ATSUSHI, SHIMOKORIYAMA, MASAMI
Comparisons of respiratory metabolism among the phloem, cambium and xylem of mature carrot root were carried out and the following results were obtained. 1) The activity of O2 uptake on a dry weight...
Fukuda, Hiroo, Komamine, Atsushi
Single cells were isolated mechanically from the mesophyll of adult plants and of seedlings of Zinnia elegans L. cv. Canary bird. When single cells isolated from the first leaves of seedlings were...
Fukuda, Hiroo, Komamine, Atsushi
A serial observation of the process of tracheary element differentiation from single cells isolated from the mesophyll of Zinnia elegans L. cv. Canary bird provided the first direct evidence for the...
Synchronization of Somatic Embryogenesis in a Carrot Cell Suspension Culture
Fujimura, Tatsuhito, Komamine, Atsushi
Synchronization of somatic embryogenesis was achieved in a carrot (Daucus carota L. cv. “Kurodagosun”) suspension culture by sieving the initial heterogeneous cell population, by density gradient...
Kodama, Hiroaki, Kawakami, Naoto, Watanabe, Akira, Komamine, Atsushi
This study shows an overall analysis of gene expression during the cell cycle in synchronous suspension cultures of Catharanthus roseus cells. First, the cellular cytoplasmic proteins were...
Nomura, Koji, Komamine, Atsushi
A system was established in which single cells differentiated to embryos at a high frequency. Small spherical single cells from a carrot (Daucus carota L. cv “Kurodagosun”) cell suspension...
Kodama, Hiroaki, Ito, Masaki, Hattori, Tsukaho, Nakamura, Kenzo, Komamine, Atsushi
A cDNA library was screened for genes that may be involved in the progression of the cell cycle of cells of higher plants. The Catharanthus roseus L. (G) Don. cells were synchronized by the double...
Polyamines and the Cell Cycle of Catharanthus roseus Cells in Culture 1
Maki, Hisae, Ando, Satoshi, Kodama, Hiroaki, Komamine, Atsushi
Investigation was made on the effect of partial depletion of polyamines (PAs), induced by treatment with inhibitors of the biosynthesis of PAs, on the distribution of cells at each phase of the cell...
Sakano, Katsuhiro, Komamine, Atsushi
Two isofunctional aspartokinases (EC 2.7.2.4) exist in fresh root tissue of carrot (Daucus carota, cv. Oogata sanzun). The threoninesensitive portion constitutes about 70% of the activity; the...
Fukuda, Hiroo, Komamine, Atsushi
Single cells were isolated mechanically from the mesophyll of adult plants and of seedlings of Zinnia elegans L. cv. Canary bird. When single cells isolated from the first leaves of seedlings were...
Fukuda, Hiroo, Komamine, Atsushi
A serial observation of the process of tracheary element differentiation from single cells isolated from the mesophyll of Zinnia elegans L. cv. Canary bird provided the first direct evidence for the...
Synchronization of Somatic Embryogenesis in a Carrot Cell Suspension Culture
Fujimura, Tatsuhito, Komamine, Atsushi
Synchronization of somatic embryogenesis was achieved in a carrot (Daucus carota L. cv. “Kurodagosun”) suspension culture by sieving the initial heterogeneous cell population, by density gradient...
Kodama, Hiroaki, Kawakami, Naoto, Watanabe, Akira, Komamine, Atsushi
This study shows an overall analysis of gene expression during the cell cycle in synchronous suspension cultures of Catharanthus roseus cells. First, the cellular cytoplasmic proteins were...
Nomura, Koji, Komamine, Atsushi
A system was established in which single cells differentiated to embryos at a high frequency. Small spherical single cells from a carrot (Daucus carota L. cv “Kurodagosun”) cell suspension...
Kodama, Hiroaki, Ito, Masaki, Hattori, Tsukaho, Nakamura, Kenzo, Komamine, Atsushi
A cDNA library was screened for genes that may be involved in the progression of the cell cycle of cells of higher plants. The Catharanthus roseus L. (G) Don. cells were synchronized by the double...
Polyamines and the Cell Cycle of Catharanthus roseus Cells in Culture 1
Maki, Hisae, Ando, Satoshi, Kodama, Hiroaki, Komamine, Atsushi
Investigation was made on the effect of partial depletion of polyamines (PAs), induced by treatment with inhibitors of the biosynthesis of PAs, on the distribution of cells at each phase of the cell...
Sakano, Katsuhiro, Komamine, Atsushi
Two isofunctional aspartokinases (EC 2.7.2.4) exist in fresh root tissue of carrot (Daucus carota, cv. Oogata sanzun). The threoninesensitive portion constitutes about 70% of the activity; the...