Atsushi SAKAI

Reduction of Coupling Effects by Optimizing the 3-D Configuration of the Routing Grid (2008)

Atsushi Sakai, Takashi Yamada, Yoshifumi Matsushita, Hiroto Yasuura

Abstract—In this brief, we propose a new physical design technique for a subquarter micrometer system-on-a-chip (SoC). By optimizing the individual layer’s routing grid space, coupling effects...

Routing Methodology for Minimizing Interconnect Energy Dissipation (2008)

Atsushi Sakai, Takashi Yamada, Yoshifumi Matsushita

In this paper, we propose a new physical design technique for sub-quarter micron system-on-a-chip (SoC). By optimizing the routing grid configuration for the automatic place and route methodology,...

NtPolI-like1 and NtPolI-like2, Bacterial DNA Polymerase I Homologs Isolated from BY-2 Cultured Tobacco Cells, Encode DNA Polymerases Engaged in DNA Replication in Both Plastids and Mitochondria (2007)

Ono, Yuriko, Sakai, Atsushi, Takechi, Katsuaki, Takio, Susumu, Takusagawa, Mari, Takano, Hiroyoshi

Two cDNAs encoding homologs of bacterial DNA polymerase I were isolated from cultured tobacco (Nicotiana tabacum) BY-2 cells, and the corresponding genes were named NtPolI-like1 and NtPolI-like2....

Reduction of Coupling Effects by Optimizing the 3-D Configuration of the Routing Grid (2003)

Sakai, Atsushi, Yamada, Takashi, Matsushita, Yoshifumi, Yasuura, Hiroto, 坂井, 篤, 山田, 節, ...

In this brief, we propose a new physical design technique for a subquarter micrometer system-on-a-chip (SoC). By optimizing the individual layer’s routing grid space, coupling effects such as...

Routing Methodology for Minimizing lnterconnect Energy Dissipation (2003)

Sakai, Atsushi, Yamada, Takashi, Matsushita, Yoshifumi, Yasuura, Hiroto, 坂井, 篤, 山田, 節, ...

In this paper, we propose a new physical design technique for sub-quarter micron system-on-a-chip (SoC). By optimizing the routing grid configuration for the automatic place and route methodology,...

Reduction of crosstalk noise by optimizing 3-D configuration of the routing grid (2003)

Sakai, Atsushi, Yamada, Takashi, Matsushita, Yoshifumi, Yasuura, Hiroto, 坂井, 篤, 山田, 孝, ...

In this paper, we propose novel physical design techniques for a sub-quarter micron system-on-a-chip (SoC). By appropriately optimizing the routing grid space or the cell utilization ratio, the...

Activation of cell proliferation by brassinolide application in tobacco BY-2 cells: effects of brassinolide on cell multiplication, cell-cycle-related gene expression, and organellar DNA contents (2003)

Miyazawa, Yutaka, Nakajima, Naoko, Abe, Tomoko, Sakai, Atsushi, Fujioka, Shozo, Kawano, Shigeyuki, ...

Brassinosteroids (BRs) are steroidal phytohormones that are essential for many processes in plant growth and development, such as cell expansion, vascular differentiation, and responses to stress....

Effects of Antibiotics that Inhibit the Bacterial Peptidoglycan Synthesis Pathway on Moss Chloroplast Division (2003)

Katayama, Nami, Takano, Hiroyoshi, Sugiyama, Motoji, Takio, Susumu, Sakai, Atsushi, Tanaka, Kan, ...

Moss chloroplasts should prove useful for studying the cyanobacteria-derived system in chloroplasts. To determine the effects of antibiotics that inhibit bacterial peptidoglycan synthesis, the...

Isolation and expression of a novel starch-storing cell-specific gene containing the KH RNA binding domain from tobacco-cultured cells BY-2 (2002)

Miyazawa, Yutaka, Sakai, Atsushi, Matsunaga, Sachihiro, Asami, Tadao, Kawano, Shigeyuki, Kuroiwa, Tsuneyoshi, ...

In cultured Bright Yellow‐2 tobacco (Nicotiana tabacum) cells, the depletion of 2,4‐dichlorophenoxyacetic acid (2,4‐D) in the culture medium induces amyloplast development. This...

Two Types of ftsZ Genes Isolated from the Unicellular Primitive Red Alga Galdieria sulphuraria (1999)

Takahara, Manabu, Takahashi, Hidenori, Matsunaga, Sachihiro, Sakai, Atsushi, Kawano, Shigeyuki, Kuroiwa, Tsuneyoshi

FtsZ plays a crucial role in bacterial cell division, and may be involved in plastid division in eukaryotes. To investigate the evolution of the dividing apparatus from prokaryotes to eukaryotes, the...

Semi-Automatic Laser Beam Microdissection of the Y Chromosome and Analysis of Y Chromosome DNA in a Dioecious Plant, Silene latifolia (1999)

Matsunaga, Sachihiro, Kawano, Shigeyuki, Michimoto, Takeshi, Higashiyama, Tetsuya, Nakao, Shunsuke, Sakai, Atsushi, ...

Silene latifolia has heteromorphic sex chromosomes, the X and Y chromosomes. The Y chromosome, which is thought to carry the male determining gene, was isolated by UV laser microdissection and...

Comparative Analysis of Plastid Gene Expression in Tobacco Chloroplasts and Proplastids: Relationship between Transcription and Transcript Accumulation (1998)

Sakai, Atsushi, Suzuki, Takeshi, Miyazawa, Yutaka, Kawano, Shigeyuki, Nagata, Toshiyuki, Kuroiwa, Tsuneyoshi

We compared the modes of plastid gene expression between chloroplasts and proplastids in tobacco (Nicotiana tabacum L.). Chloroplast-nuclei (chloroplast-nucleoids) and proplastid-nuclei...

Transcriptional Activities of the Chloroplast-Nuclei and Proplastid-Nuclei Isolated from Tobacco Exhibit Different Sensitivities to Tagetitoxin: Implication of the Presence of Distinct RNA Polymerases (1998)

Sakai, Atsushi, Saito, Chieko, Inada, Noriko, Kuroiwa, Tsuneyoshi

We examined the effects of tagetitoxin, a potent inhibitor of RNA polymerases from chloroplasts and Escherichia coli, on the transcriptional activities of chloroplast- and proplastid-nuclei...

Matching for affined transformed pictures using Hough planes (1996)

Atsushi Sakai, Yoshihiko Nomura, Yasunaga Mitsuya

Considering the relationship between the deformation on Hough planes and the affine transformation on raw images, the authors have proposed a matching algorithm; four-dimensional optimization problem...

Localization of organelle DNA synthesis within the root apical meristem of rice (1995)

Suzuki, Takeshi, Sasaki, Narie, Sakai, Atsushi, Kawano, Shigeyuki, Kuroiwa, Tsuneyoshi

DNA synthesis in cell nuclei and organelles in the root apical meristem of rice was analysed by anti-BrdU immunofluorescence microscopy to determine whether there is a specific order of these events...

Transcriptional Activity of Morphologically Intact Proplastid-Nuclei (Nucleoids) Isolated from Tobacco Cultured Cells (1991)

Sakai, Atsushi, Yamashita, Hirofumi, Nemoto, Yasuyuki, Kawano, Shigeyuki, Kuroiwa, Tsuneyoshi

The transcriptional activity of morphologically intact proplastid-nuclei (synonymous with proplastid-nucleoids) isolated from cultured tobacco cells (line BY-2) was examined by an assay of...

Auxin and Cytokinin Have Opposite Effects on Amyloplast Development and the Expression of Starch Synthesis Genes in Cultured Bright Yellow-2 Tobacco Cells1

Miyazawa, Yutaka, Sakai, Atsushi, Miyagishima, Shin-ya, Takano, Hiroyoshi, Kawano, Shigeyuki, Kuroiwa, Tsuneyoshi

In cultured Bright Yellow-2 (BY-2) tobacco (Nicotiana tabacum) cells, the depletion of auxin (2,4-dichlorophenoxyacetic acid) in the culture medium induces the accumulation of starch. This is...

Glom Is a Novel Mitochondrial DNA Packaging Protein in Physarum polycephalum and Causes Intense Chromatin Condensation without Suppressing DNA Functions

Sasaki, Narie, Kuroiwa, Haruko, Nishitani, Chikako, Takano, Hiroyoshi, Higashiyama, Tetsuya, Kobayashi, Tamaki, ...

Mitochondrial DNA (mtDNA) is packed into highly organized structures called mitochondrial nucleoids (mt-nucleoids). To understand the organization of mtDNA and the overall regulation of its genetic...

Conversion of Proplastids to Amyloplasts in Tobacco Cultured Cells Is Accompanied by Changes in the Transcriptional Activities of Plastid Genes 1

Sakai, Atsushi, Kawano, Shigeyuki, Kuroiwa, Tsuneyoshi

When tobacco (Nicotiana tabacum L.) cultured cells (line BY-2) at the stationary phase were transferred to culture medium that contained cytokinin (benzyladenine) instead of auxin...

Auxin and Cytokinin Have Opposite Effects on Amyloplast Development and the Expression of Starch Synthesis Genes in Cultured Bright Yellow-2 Tobacco Cells1

Miyazawa, Yutaka, Sakai, Atsushi, Miyagishima, Shin-ya, Takano, Hiroyoshi, Kawano, Shigeyuki, Kuroiwa, Tsuneyoshi

In cultured Bright Yellow-2 (BY-2) tobacco (Nicotiana tabacum) cells, the depletion of auxin (2,4-dichlorophenoxyacetic acid) in the culture medium induces the accumulation of starch. This is...

Glom Is a Novel Mitochondrial DNA Packaging Protein in Physarum polycephalum and Causes Intense Chromatin Condensation without Suppressing DNA Functions

Sasaki, Narie, Kuroiwa, Haruko, Nishitani, Chikako, Takano, Hiroyoshi, Higashiyama, Tetsuya, Kobayashi, Tamaki, ...

Mitochondrial DNA (mtDNA) is packed into highly organized structures called mitochondrial nucleoids (mt-nucleoids). To understand the organization of mtDNA and the overall regulation of its genetic...

Conversion of Proplastids to Amyloplasts in Tobacco Cultured Cells Is Accompanied by Changes in the Transcriptional Activities of Plastid Genes 1

Sakai, Atsushi, Kawano, Shigeyuki, Kuroiwa, Tsuneyoshi

When tobacco (Nicotiana tabacum L.) cultured cells (line BY-2) at the stationary phase were transferred to culture medium that contained cytokinin (benzyladenine) instead of auxin...

Decreased expression of glial cell line-derived neurotrophic factor signaling in rat models of neuropathic pain

Nagano, Masatoshi, Sakai, Atsushi, Takahashi, Naoki, Umino, Masahiro, Yoshioka, Koichi, Suzuki, Hidenori

In an attempt to clarify whether glial cell line-derived neurotrophic factor (GDNF), a survival factor for subpopulations of primary afferent neurons, is involved in the states of neuropathic pain,...