Su, Bo, Liu, Haihua, Wang, Xinglong, Chen, Shu G, Siedlak, Sandra L, Kondo, Eisaku, ...
ABSTRACT: Lewy bodies and Lewy neurites constitute the cardinal neuropathological features of both Parkinson's disease (PD) and Lewy body dementia (LBD). Whereas alpha-synuclein has been found to be...
Abe, Nobuhito, Fujii, Toshikatsu, Hirayama, Kazumi, Takeda, Atsushi, Hosokai, Yoshiyuki, Ishioka, Toshiyuki, ...
Parkinson's disease is a common neurodegenerative disorder with both motor symptoms and cognitive deficits such as executive dysfunction. Over the past 100 years, a growing body of literature has...
Sublethal RNA Oxidation as a Mechanism for Neurodegenerative Disease (2008)
Rudy J. Castellani, Akihiko Nunomura, Raj K. Rolston, Paula I. Moreira, Atsushi Takeda, George Perry, ...
Although cellular RNA is subjected to the same oxidative insults as DNA and other cellular macromolecules, oxidative damage to RNA has not been a major focus in investigations of the biological...
Takeda, Atsushi, Iwasaki, Shintaro, Watanabe, Toshiaki, Utsumi, Maki, Watanabe, Yuichiro
Double-stranded RNA induces RNA silencing and is cleaved into 21–24 nt small RNA duplexes by Dicer enzyme. A strand of Dicer-generated small RNA duplex (called the guide strand) is then selected by...
Direction-sensitive dark matter search results in a surface laboratory (2007)
Miuchi, Kentaro, Hattori, Kaori, Kabuki, Shigeto, Kubo, Hidetoshi, Kurosawa, Shunsuke, Nishimura, Hironobu, ...
We developed a three-dimensional gaseous tracking device and performed a direction-sensitive dark matter search in a surface laboratory. By using 150 Torr carbon-tetrafluoride (CF_4 gas), we obtained...
Kamata, Yayoi, Itoh, Yoshiko, Kajiya, Akane, Karasawa, Sachiyo, Sakatani, Chie, Takekoshi, Susumu, ...
Kamata, Yayoi, Itoh, Yoshiko, Kajiya, Akane, Karasawa, Sachiyo, Sakatani, Chie, Takekoshi, Susumu, ...
A neutral cysteine protease, bleomycin hydrolase (BH), was found to be present in the range 3.7–131.1 ng per mg of rat tissues by enzyme-lined immunosorbent assay (ELISA). Newborn rat skin...
Detector performance of the NEWAGE experiment (2007)
Miuchi, Kentaro, Hattori, Kaori, Kabuki, Shigeto, Kubo, Hidetoshi, Kurosawa, Shunsuke, Nishimura, Hironobu, ...
NEWAGE(NEw generation WIMP search with an Advanced Gaseous tracking device Experiment) project is a direction-sensitive dark matter search experiment with a gaseous micro...
Oxidative damage to RNA in neurodegenerative diseases. (2006)
Nunomura, Akihiko, Honda, Kazuhiro, Takeda, Atsushi, Hirai, Keisuke, Zhu, Xiongwei, Smith, Mark A., ...
Since 1999, oxidative damage to RNA molecules has been described in several neurological diseases including Alzheimer's disease, Parkinson's disease, Down syndrome, dementia with Lewy bodies, prion...
Mechanisms of Neuronal Death in Synucleinopathy (2006)
Atsushi Takeda, Takafumi Hasegawa, Michiko Matsuzaki-Kobayashi, Naoto Sugeno, Akio Kikuchi, Yasuto Itoyama, ...
α-synuclein is a key molecule in the pathogenesis of synucleinopathy including Parkinson's disease and multiple system atrophy. In this mini-review, we mainly focus on recent data obtained from...
Oxidative Damage to RNA in Neurodegenerative Diseases (2006)
Akihiko Nunomura, Kazuhiro Honda, Atsushi Takeda, Keisuke Hirai, Xiongwei Zhu, Mark A. Smith, ...
Since 1999, oxidative damage to RNA molecules has been described in several neurological diseases including Alzheimer's disease, Parkinson's disease, Down syndrome, dementia with Lewy bodies, prion...
Watanabe, Toshiaki, Takeda, Atsushi, Tsukiyama, Tomoyuki, Mise, Kazuyuki, Okuno, Tetsuro, Sasaki, Hiroyuki, ...
Small RNAs ranging in size between 18 and 30 nucleotides (nt) are found in many organisms including yeasts, plants, and animals. Small RNAs are involved in the regulation of gene expression through...
Kamata, Yayoi, Itoh, Yoshiko, Kajiya, Akane, Karasawa, Sachiyo, Sakatani, Chie, Takekoshi, Susumu, ...
A neutral cysteine protease, bleomycin hydrolase (BH), was found to be present in the range 3.7-131.1 ng per mg of rat tissues by enzyme-linked immunosorbent assay (ELISA). Newborn rat skin contained...
Simulation study of electron drift and gas multiplication in Micro Pixel Chamber (2005)
Nagayoshi, Tsutomu, Takada, Atsushi, Kubo, Hidetoshi, Miuchi, Kentaro, Orito, Reiko, Okada, Yoko, ...
The physical processes of charge collection and gas multiplication of a Micro Pixel Chamber (mu-PIC) were studied in detail using a three-dimensional simulation. The collection efficiencies of...
Wohlschlaeger, Jeremias, Schmitz, Klaus J., Schmid, Christof, Schmid, Kurt W., Keul, Petra, Takeda, Atsushi, ...
Left ventricular assist devices (LVAD) are used to "bridge" patients with end-stage heart failure until transplantation of a donor heart can be performed ("bridge to transplantation"). However, in a...
MeV Gamma-Ray Imaging Detector with micro-TPC (2004)
Tanimori, Toru, Kubo, Hidetoshi, Miuchi, Kentaro, Nagayoshi, Tsutomu, Orito, Reiko, Takada, Atsushi, ...
We propose a new imaging gamma-ray detector in the MeV region. By measuring the directions and energies of not only a scattered gamma ray but also a recoil electron, the direction of an incident...
Detecting the WIMP-wind via spin-dependent interactions (2003)
Tanimori, Toru, Kubo, Hidetoshi, Miuchi, Kentaro, Nagayoshi, Tsutomu, Orito, Reiko, Takada, Atsushi, ...
Revealing the nature of dark matter is one of the most interesting tasks in astrophysics. Measuring the distribution of recoil angles is said to be one of the most reliable methods to detect a...
Performance of a micro-TPC for a time-resolved neutron PSD (2003)
Miuchi, Kentaro, Kubo, Hidetoshi, Nagayoshi, Tsutomu, Orito, Reiko, Takada, Atsushi, Takeda, Atsushi, ...
We report on the performance of a micro-TPC with a micro pixel chamber($\mu$-PIC) readout for a time-resolved neutron position-sensitive detector(PSD). Three-dimensional tracks and the Bragg curves...
Baba, Hideo A., Stypmann, Jörg, Grabellus, Florian, Kirchhof, Paulus, Sokoll, Andrea, Schäfers, Michael, ...
Objective: Left ventricular assist devices (LVAD) are used to bridge patients with end-stage heart failure to transplantation. After long-term LVAD support, ventricular function may partially...
Grabellus, Florian, Levkau, Bodo, Sokoll, Andrea, Welp, Hendryk, Schmid, Christof, Deng, Mario C, ...
Objective: Left ventricular assist devices (LVAD) have been used to ‘bridge’ patients with end-stage heart failure to transplantation. Although several reports have suggested that the native...
Kaji, Hiroyuki, Yada-Wakatabe, Rumi, Uehira, Takashi, Terai, Mayumi, Takeda, Atsushi, Satoh, Takanori, ...
A rat cystatin A cDNA clone was isolated from a λ ZAP library representing newborn rat skin mRNA by screening with a synthetic oligonucleotide designed from amino acid sequence 15–23 of the...
Detection of OXA-4 {beta}-lactamase in Pseudomonas aeruginosa isolates by genetic methods (1999)
Marumo, Kenji, Takeda, Atsushi, Nakamura, Yoshiko, Nakaya, Kazuyasu
In Pseudomonas aeruginosa, resistance to cefclidin is usually associated with resistance to another third-generation cephalosporin, ceftazidime. In this study we analysed 22 isolates of P....
Takeda, Atsushi, Higuchi, Dousei, Yamamoko, Takako, Nakamura, Yoshiko, Masuda, Yutaka, Hirabayashi, Takahiro, ...
Bleomycin (BLM) hydrolase, which hydrolyzes the carboxyamide bond in the β-amino-alanine moiety, was purified from newborn rat skin. The enzyme was purified 2,500-fold over the crude extract to...
Takeda, Atsushi, Masuda, Yutaka, Yamamoto, Takako, Hirabayashi, Takahiro, Nakamura, Yoshiko, Nakaya, Kazuyasu
We isolated and characterized almost the entire cDNA encoding BLM hydrolase from rat spleen cDNA libraries. The cDNA encoded a polypeptide composed of 454 amino acids, that had a slightly larger...
STRONG IMMUNOREACTIVITY OF CATHEPSIN L AT THE SITE OF RIMMED VACUOLES IN DISEASED MUSCLES (1992)
JIMI, TAKAHIRO, SATOH, YUTAKA, TAKEDA, ATSUSHI, SHIBUYA, SEIJI, WAKAYAMA, YOSHIHIRO, SUGITA, KOUJIRO
Skeletal muscle samples from four patients with myopathies showing autophagic vacuole formation were examined by immunohistochemical, biochemical and immunoblot analyses. Immunochemical studies...
Takeda, Atsushi, Kaji, Hiroyuki, Nakaya, Kazuyasu, Nakamura, Yasuharu, Samejima, Tatsuya
We have studied the primary structure of human cystatin As from epidermis, liver, spleen, and leukocytes. These molecules were indistinguishable on PAGE in the presence and absence of SDS, by fast...
Characterization of Copper Atoms in Bilirubin Oxidase by Spectroscopic Analyses (1989)
Gotoh, Yasuasa, Kondo, Yoshifusa, Kaji, Hiroyuki, Takeda, Atsushi, Samejima, Tatsuya
Bilirubin oxidase [EC 1.3.3.5], purified from the culture medium of Myrothecium verrucaria, was found to contain two blue copper atoms per protein molecule with a molecular weight of ca, 52 kDa. The...
Studies on Chemical Synthesis of Human Cystatin A Gene and Its Expression in Escherichia coli (1989)
Kaji, Hiroyuki, Kumagai, Izumi, Takeda, Atsushi, Miura, Kin-ichiro, Samejima, Tatsuya
A synthetic gene containing the coding sequence for the human cysteine proteinase inhibitor, cystatin A, was obtained by enzymatic assembly of 20 oligodeoxyribonucleotides which had been chemically...
Samejima, Tatsuya, Tamagawa, Yuko, Kondo, Yoshifusa, Hachimori, Akira, Kaji, Hiroyuki, Takeda, Atsushi, ...
Chemical modifications by photooxidation in the presence of rose bengal (RB) and with tetranitromethane (TNM) were carried out to elucidate the amino acid residues involved in the active site of...
TAKEDA, Atsushi, KOBAYASHI, Shu, KAJI, Hiroyuki, AOKI, Yoshio, SAMEJIMA, Tatsuya
The conformational changes of the papain molecule on interaction with two thiol proteinase inhibitors (TPI(1) and TPI(2)) from newborn rat epidermis were studied by measuring circular dichroism (CD),...
TAKEDA, Atsushi, KOBAYASHI, Shu, SAMEJIMA, Tatsuya
Two low-molecular-weight thiol proteinase inhibitors, TPI(l) and TPI(2), were purified from newborn rat epidermis by homogenization and extraction at 37�C, followed by lyophilization, Sephadex G-75...
TAKEDA, Atsushi, HIRANO, Kouichi, SHIROYA, Yoko, SAMEJIMA, Tatsuya
The dissociation of porcine erythrocyte catalase [EC 1.11.1.6] into subunits on denaturation with alkali, GuHCl and urea was investigated by following the changes in hydrodynamic properties,...
On the Acid Denaturation of Porcine Erythrocyte Catalase in Relation to Its Subunit Structure (1981)
SAMEJIMA, Tatsuya, MIYAHARA, Tsuneo, TAKEDA, Atsushi, HACHIMORI, Akira, HIRANO, Kouichi
Porcine erythrocyte catalase [EC 1.11.1.6] (molecular weight, ca. 250, 000) was found to dissociate partially between pH 3.5 and 3.0 and completely below pH 3.0 into two presumably identical...
The Interactions of Thiol Compounds with Porcine Erythrocyte Catalase (1980)
TAKEDA, Atsushi, MIYAHARA, Tsuneo, HACHIMORI, Akira, SAMEJIMA, Tatsuya
The effects of thiol compounds on the conformation of porcine crythrocyte catalase were examined. The thiol compounds showed two types of reactivity with the catalase in terms of changes in...
NAGAOKA, Tsuneyuki, HACHIMORI, Akira, TAKEDA, Atsushi, SAMEJIMA, Tatsuya
A new method of affinity chromatography using blue dextran-Sepharose 4B resin was established to purify NADP+-dependent isocitrate dehydrogenase [EC 1.1 1 42] from Bacillus stearothermophilus in high...
On the Characterization of Porcine Erythrocyte Catalase and Its Disulfide-Linked Dimer (1977)
TAKEDA, Atsushi, SAMEJIMA, Tatsuya
Purified native erythrocyte catalase [EC 1.11.1.6] (monomer) associates into dimer, trimer, tetramer and larger molecules by forming disulfide cross-links due to air oxidation of SH groups in the...
Effect of Sodium Dodecyl Sulfate on the Dissociation of Bovine Liver Catalase (1975)
TAKEDA, Atsushi, HACHIMORI, Akira, MURAL, Mariko, SATO, Katsumi, SAMEJIMA, Tatsuya
Native bovine liver catalase [EC 1.11.1.6] and catalase acetylated with N-acetylim-idazole (AI) both combined with sodium dodecyl sulfate (SDS) to form catalase-SDS complexes. The differences between...
HACHIMORI, Akira, TAKEDA, Atsushi, KAIBUCHI, Minoru, OHKAWARA, Nobuyasu, SAMEJIMA, Tatsuya
Inorganic pyrophosphatase [EC 3. 6.1.1] was purified from Bacillus stearothermophilus to a homogeneous state both ultracentrifugally and electrophoretically. Ultracentrif-ugal analysis revealed that...
HACHIMORI, Akira, TAKEDA, Atsushi, NAGAOKA, Tsuneyuki, SUZUKI, Harumi, NOSOH, Yoshiaki, SAMEJIMA, Tatsuya
The SH groups of glutamine synthetase [EC 6.3.1.2] from Bacillus stearothermophilus were modified with 5, 5'-dithiobis(2-nitrobenzoic acid) in order to determine the number of SH groups in the...
A plant RNA virus suppresses RNA silencing through viral RNA replication
Takeda, Atsushi, Tsukuda, Misato, Mizumoto, Hiroyuki, Okamoto, Kimiyuki, Kaido, Masanori, Mise, Kazuyuki, ...
RNA interference (RNAi) is a post-transcriptional gene-regulatory mechanism that operates in many eukaryotes. RNAi is induced by double-stranded RNA (dsRNA) and is mainly involved in defence against...
Mechanisms of Neuronal Death in Synucleinopathy
Takeda, Atsushi, Hasegawa, Takafumi, Matsuzaki-Kobayashi, Michiko, Sugeno, Naoto, Kikuchi, Akio, Itoyama, Yasuto, ...
α-synuclein is a key molecule in the pathogenesis of synucleinopathy including Parkinson's disease and multiple system atrophy. In this mini-review, we mainly focus on recent data obtained from...
Yamanaka, Tsutomu, Takeda, Atsushi, Shimada, Jun
研究概要:Field measurements of soil water content, temperature, and relative humidity were made at several depths within a lysimeter where the dry surface layer (DSL) formed. The observed water...
Yamanaka, Tsutomu, Takeda, Atsushi, Shimada, Jun
研究概要:Field measurements of soil water content, temperature, and relative humidity were made at several depths within a lysimeter where the dry surface layer (DSL) formed. The observed water...
Oxidative Damage to RNA in Neurodegenerative Diseases
Nunomura, Akihiko, Honda, Kazuhiro, Takeda, Atsushi, Hirai, Keisuke, Zhu, Xiongwei, Smith, Mark A., ...
Since 1999, oxidative damage to RNA molecules has been described in several neurological diseases including Alzheimer's disease, Parkinson's disease, Down syndrome, dementia with Lewy bodies, prion...
A plant RNA virus suppresses RNA silencing through viral RNA replication
Takeda, Atsushi, Tsukuda, Misato, Mizumoto, Hiroyuki, Okamoto, Kimiyuki, Kaido, Masanori, Mise, Kazuyuki, ...
RNA interference (RNAi) is a post-transcriptional gene-regulatory mechanism that operates in many eukaryotes. RNAi is induced by double-stranded RNA (dsRNA) and is mainly involved in defence against...
Mechanisms of Neuronal Death in Synucleinopathy
Takeda, Atsushi, Hasegawa, Takafumi, Matsuzaki-Kobayashi, Michiko, Sugeno, Naoto, Kikuchi, Akio, Itoyama, Yasuto, ...
α-synuclein is a key molecule in the pathogenesis of synucleinopathy including Parkinson's disease and multiple system atrophy. In this mini-review, we mainly focus on recent data obtained from...
Oxidative Damage to RNA in Neurodegenerative Diseases
Nunomura, Akihiko, Honda, Kazuhiro, Takeda, Atsushi, Hirai, Keisuke, Zhu, Xiongwei, Smith, Mark A., ...
Since 1999, oxidative damage to RNA molecules has been described in several neurological diseases including Alzheimer's disease, Parkinson's disease, Down syndrome, dementia with Lewy bodies, prion...
Watanabe, Toshiaki, Takeda, Atsushi, Tsukiyama, Tomoyuki, Mise, Kazuyuki, Okuno, Tetsuro, Sasaki, Hiroyuki, ...
Small RNAs ranging in size between 18 and 30 nucleotides (nt) are found in many organisms including yeasts, plants, and animals. Small RNAs are involved in the regulation of gene expression through...
Yamanaka, Tsutomu, Takeda, Atsushi, Shimada, Jun
研究概要:Field measurements of soil water content, temperature, and relative humidity were made at several depths within a lysimeter where the dry surface layer (DSL) formed. The observed water...
Schmitz, Klaus J, Lang, Hauke, Frey, Ulrich H, Sotiropoulos, Georgios C, Wohlschlaeger, Jeremias, Reis, Henning, ...
Sublethal RNA Oxidation as a Mechanism for Neurodegenerative Disease
Castellani, Rudy J., Nunomura, Akihiko, Rolston, Raj K., Moreira, Paula I., Takeda, Atsushi, Perry, George, ...
Although cellular RNA is subjected to the same oxidative insults as DNA and other cellular macromolecules, oxidative damage to RNA has not been a major focus in investigations of the biological...
Do parkinsonian patients have trouble telling lies? The neurobiological basis of deceptive behaviour
Abe, Nobuhito, Fujii, Toshikatsu, Hirayama, Kazumi, Takeda, Atsushi, Hosokai, Yoshiyuki, Ishioka, Toshiyuki, ...
Parkinson's disease is a common neurodegenerative disorder with both motor symptoms and cognitive deficits such as executive dysfunction. Over the past 100 years, a growing body of literature has...
Ectopic localization of FOXO3a protein in Lewy bodies in Lewy body dementia and Parkinson's disease
Su, Bo, Liu, Haihua, Wang, Xinglong, Chen, Shu G, Siedlak, Sandra L, Kondo, Eisaku, ...
Lewy bodies and Lewy neurites constitute the cardinal neuropathological features of both Parkinson's disease (PD) and Lewy body dementia (LBD). Whereas α-synuclein has been found to be the major...