Bradford, P A, Urban, C, Jaiswal, A, Mariano, N, Rasmussen, B A, Projan, S J, ...
Four ceftazidime-resistant Escherichia coli strains were isolated from elderly nursing home patients in a New York hospital during 1993. Strains MCQ-2, MCQ-3, and MCQ-4 were determined to be...
Rasmussen, B A, Bush, K, Keeney, D, Yang, Y, Hare, R, O'Gara, C, ...
In 1984, a year prior to the U.S. approval of imipenem for clinical use, a wound isolate and a bile isolate of Enterobacter cloacae were obtained from two patients in a California hospital. These...
Cloning and sequencing of the class B beta-lactamase gene (ccrA) from Bacteroides fragilis TAL3636.
Rasmussen, B A, Gluzman, Y, Tally, F P
Bacteroides fragilis TAL3636 produces a class B, Zn2(+)-requiring beta-lactamase. The gene, ccrA, was cloned and expressed in Escherichia coli. The gene was sequenced and shown to share greater than...
Alksne, L E, Keeney, D, Rasmussen, B A
The metallo-beta-lactamase gene, ccrA, from Bacteroides fragilis is functionally expressed in Escherichia coli only in the presence of a genomic mutation in iarA or iarB (increased ampicillin...
It has previously been shown that functional expression of CcrA, a metallo-beta-lactamase from Bacteroides fragilis, in Escherichia coli requires a mutation in either dsbA or dsbB, components of a...
Aeromonas jandaei AER 14 (formerly Aeromonas sobria AER 14) expresses three inducible beta-lactamases, AsbA1, OXA-12 (AsbB1), and AsbM1. Mutant strains that constitutively overexpress all three...
Kinetic interactions of tazobactam with beta-lactamases from all major structural classes.
Bush, K, Macalintal, C, Rasmussen, B A, Lee, V J, Yang, Y
Tazobactam was shown to be a potent inhibitor of group 1, 2a, 2b, and 2b' beta-lactamases. Extended kinetic studies with class A and C serine beta-lactamases showed that the PC1, TEM-2, and P99...
Rasmussen, B A, Bradford, P A, Quinn, J P, Wiener, J, Weinstein, R A, Bush, K
Ceftazidime-resistant isolates of Escherichia coli and Klebsiella pneumoniae produced a plasmid-mediated beta-lactamase with a pI of 5.6 with biochemical characteristics comparable to those of the...
Biochemical characterization of the metallo-beta-lactamase CcrA from Bacteroides fragilis TAL3636.
Yang, Y, Rasmussen, B A, Bush, K
The CcrA beta-lactamase from Bacteroides fragilis TAL3636 was cloned into Escherichia coli and purified from inclusion bodies. This group 3 metalloenzyme hydrolyzed most beta-lactam antibiotics,...
Benson, S A, Hall, M N, Rasmussen, B A
The lamB701-708 signal sequence mutation reduces expression of LamB, an outer membrane protein of Escherichia coli. To investigate the possibility that synthesis and export of LamB are coupled, as...
Rasmussen, B A, MacGregor, C H, Ray, P H, Bassford, P J
It has not been possible to obtain in vitro expression of the positively regulated malE gene encoding the periplasmic maltose-binding protein (MBP) of Escherichia coli. To facilitate in vitro malE...
Export and processing of MalE-LacZ hybrid proteins in Escherichia coli.
Rasmussen, B A, Bankaitis, V A, Bassford, P J
Five classes of MalE-LacZ hybrid proteins have previously been characterized. These proteins differ in the amount of the maltose-binding protein (MBP) that is attached to beta-galactosidase. Although...
It previously has been demonstrated that synthesis of the periplasmic maltose-binding protein (MBP) and alkaline phosphatase (AP) of Eschericha coli predominantly occurs on membrane-bound polysomes....
Urban, C, Meyer, K S, Mariano, N, Rahal, J J, Flamm, R, Rasmussen, B A, ...
An epidemic of nosocomial ceftazidime-resistant Klebsiella pneumoniae was correlated with production of a ceftazidime-hydrolyzing enzyme with an isoelectric point of 5.6 (BMH-1). BMH-1 was encoded on...
Bradford, P A, Cherubin, C E, Idemyor, V, Rasmussen, B A, Bush, K
Ceftazidime-resistant Klebsiella pneumoniae strains began to appear when ceftazidime usage was increased in two unrelated Chicago hospitals. These strains produced a beta-lactamase with an...
Rasmussen, B A, Gluzman, Y, Tally, F P
One of the two major mechanisms of tetracycline resistance is ribosomal protection. Of this resistance type, tet(M) is the best characterized. Although the mechanism of tet(M) resistance has not yet...
Rasmussen, B A, Keeney, D, Yang, Y, Bush, K
Two beta-lactamase genes, asbA1 and asbB1, encoding AsbA1 and AsbB1, respectively, have been cloned from Aeromonas sobria AER 14M into Escherichia coli. AsbA1 was expressed at low but detectable...
Rasmussen, B A, Yang, Y, Jacobus, N, Bush, K
The metallo-beta-lactamase gene, ccrA, has been cloned from three clinical isolates of Bacteroides fragilis, TAL3636, QMCN3, and QMCN4. Although all three isolates harbored a gene encoding a potent...
Bradford, P A, Urban, C, Jaiswal, A, Mariano, N, Rasmussen, B A, Projan, S J, ...
Four ceftazidime-resistant Escherichia coli strains were isolated from elderly nursing home patients in a New York hospital during 1993. Strains MCQ-2, MCQ-3, and MCQ-4 were determined to be...
Rasmussen, B A, Bush, K, Keeney, D, Yang, Y, Hare, R, O'Gara, C, ...
In 1984, a year prior to the U.S. approval of imipenem for clinical use, a wound isolate and a bile isolate of Enterobacter cloacae were obtained from two patients in a California hospital. These...
Cloning and sequencing of the class B beta-lactamase gene (ccrA) from Bacteroides fragilis TAL3636.
Rasmussen, B A, Gluzman, Y, Tally, F P
Bacteroides fragilis TAL3636 produces a class B, Zn2(+)-requiring beta-lactamase. The gene, ccrA, was cloned and expressed in Escherichia coli. The gene was sequenced and shown to share greater than...
Alksne, L E, Keeney, D, Rasmussen, B A
The metallo-beta-lactamase gene, ccrA, from Bacteroides fragilis is functionally expressed in Escherichia coli only in the presence of a genomic mutation in iarA or iarB (increased ampicillin...
It has previously been shown that functional expression of CcrA, a metallo-beta-lactamase from Bacteroides fragilis, in Escherichia coli requires a mutation in either dsbA or dsbB, components of a...
Aeromonas jandaei AER 14 (formerly Aeromonas sobria AER 14) expresses three inducible beta-lactamases, AsbA1, OXA-12 (AsbB1), and AsbM1. Mutant strains that constitutively overexpress all three...
Kinetic interactions of tazobactam with beta-lactamases from all major structural classes.
Bush, K, Macalintal, C, Rasmussen, B A, Lee, V J, Yang, Y
Tazobactam was shown to be a potent inhibitor of group 1, 2a, 2b, and 2b' beta-lactamases. Extended kinetic studies with class A and C serine beta-lactamases showed that the PC1, TEM-2, and P99...
Rasmussen, B A, Bradford, P A, Quinn, J P, Wiener, J, Weinstein, R A, Bush, K
Ceftazidime-resistant isolates of Escherichia coli and Klebsiella pneumoniae produced a plasmid-mediated beta-lactamase with a pI of 5.6 with biochemical characteristics comparable to those of the...
Biochemical characterization of the metallo-beta-lactamase CcrA from Bacteroides fragilis TAL3636.
Yang, Y, Rasmussen, B A, Bush, K
The CcrA beta-lactamase from Bacteroides fragilis TAL3636 was cloned into Escherichia coli and purified from inclusion bodies. This group 3 metalloenzyme hydrolyzed most beta-lactam antibiotics,...
Benson, S A, Hall, M N, Rasmussen, B A
The lamB701-708 signal sequence mutation reduces expression of LamB, an outer membrane protein of Escherichia coli. To investigate the possibility that synthesis and export of LamB are coupled, as...
Rasmussen, B A, MacGregor, C H, Ray, P H, Bassford, P J
It has not been possible to obtain in vitro expression of the positively regulated malE gene encoding the periplasmic maltose-binding protein (MBP) of Escherichia coli. To facilitate in vitro malE...
Export and processing of MalE-LacZ hybrid proteins in Escherichia coli.
Rasmussen, B A, Bankaitis, V A, Bassford, P J
Five classes of MalE-LacZ hybrid proteins have previously been characterized. These proteins differ in the amount of the maltose-binding protein (MBP) that is attached to beta-galactosidase. Although...
It previously has been demonstrated that synthesis of the periplasmic maltose-binding protein (MBP) and alkaline phosphatase (AP) of Eschericha coli predominantly occurs on membrane-bound polysomes....
Urban, C, Meyer, K S, Mariano, N, Rahal, J J, Flamm, R, Rasmussen, B A, ...
An epidemic of nosocomial ceftazidime-resistant Klebsiella pneumoniae was correlated with production of a ceftazidime-hydrolyzing enzyme with an isoelectric point of 5.6 (BMH-1). BMH-1 was encoded on...
Bradford, P A, Cherubin, C E, Idemyor, V, Rasmussen, B A, Bush, K
Ceftazidime-resistant Klebsiella pneumoniae strains began to appear when ceftazidime usage was increased in two unrelated Chicago hospitals. These strains produced a beta-lactamase with an...
Rasmussen, B A, Gluzman, Y, Tally, F P
One of the two major mechanisms of tetracycline resistance is ribosomal protection. Of this resistance type, tet(M) is the best characterized. Although the mechanism of tet(M) resistance has not yet...
Rasmussen, B A, Keeney, D, Yang, Y, Bush, K
Two beta-lactamase genes, asbA1 and asbB1, encoding AsbA1 and AsbB1, respectively, have been cloned from Aeromonas sobria AER 14M into Escherichia coli. AsbA1 was expressed at low but detectable...
Rasmussen, B A, Yang, Y, Jacobus, N, Bush, K
The metallo-beta-lactamase gene, ccrA, has been cloned from three clinical isolates of Bacteroides fragilis, TAL3636, QMCN3, and QMCN4. Although all three isolates harbored a gene encoding a potent...
Concha, N. O., Rasmussen, B. A., Bush, K., Herzberg, O.
The metallo-beta-lactamases require zinc or cadmium for hydrolyzing beta-lactam antibiotics and are inhibited by mercurial compounds. To data, there are no clinically useful inhibitors of this class...
Li, Z., Rasmussen, B. A., Herzberg, O.
The metallo-beta-lactamases require divalent cations such as zinc or cadmium for hydrolyzing the amide bond of beta-lactam antibiotics. The crystal structure of the Zn2+ -bound enzyme from...