Wickham, Mark E, Brown, Nat F, Provias, John, Finlay, B Brett, Coombes, Brian K
Abstract Background Salmonella meningitis is a rare and serious infection of the central nervous system following acute Salmonella enterica sepsis. For this pathogen, no appropriate model has been...
Wickham, Mark E., Brown, Nathaniel Francis, Provias, John, Finlay, B. Brett, Coombes, Brian K.
BACKGROUND: Salmonella meningitis is a rare and serious infection of the central nervous system following acute Salmonella enterica sepsis. For this pathogen, no appropriate model has been reported...
Wickham, Mark E., Brown, Nathaniel Francis, Provias, John, Finlay, B. Brett, Coombes, Brian K.
BACKGROUND: Salmonella meningitis is a rare and serious infection of the central nervous system following acute Salmonella enterica sepsis. For this pathogen, no appropriate model has been reported...
Virulence is positively selected by transmission success between mammalian hosts (2007)
Wickham, Mark E., Brown, Nathaniel Francis, Boyle, Erin C., Coombes, Brian K., Finlay, B. Brett
Virulence, defined as damage to the host, is a trait of pathogens that evolutionary theory suggests benefits the pathogen in the ''struggle for existence'' [1]. Pathogens employ virulence mechanisms...
Coombes, Brian K., Lowden, Michael J., Bishop, Jenna L., Wickham, Mark E., Brown, Nathaniel Francis, Duong, Nancy, ...
Bacterial pathogens use horizontal gene transfer to acquire virulence factors that influence host colonization, alter virulence traits, and ultimately shape the outcome of disease following...
SopD acts cooperatively with SopB during Salmonella enterica serovar Typhimurium invasion (2007)
Bakowski, Malina A., Cirulis, Judith T., Brown, Nathaniel Francis, Finlay, B. Brett, Brumell, John H.
The intracellular bacterial pathogen, Salmonella enterica serovar Typhimurium (S. typhimurium), causes disease in a variety of hosts. To invade and replicate in host cells, these bacteria subvert...
Boyle, Erin C., Brown, Nathaniel Francis, Brumell, John H., Finlay, B. Brett
The ability of Salmonella enterica serovar Typhimurium (S. Typhimurium) to penetrate the intestinal epithelium is key to its pathogenesis. Bacterial invasion can be seen as a two-step process...
Virulence is positively selected by transmission success between mammalian hosts (2007)
Wickham, Mark E., Brown, Nathaniel Francis, Boyle, Erin C., Coombes, Brian K., Finlay, B. Brett
Virulence, defined as damage to the host, is a trait of pathogens that evolutionary theory suggests benefits the pathogen in the ''struggle for existence'' [1]. Pathogens employ virulence mechanisms...
Coombes, Brian K., Lowden, Michael J., Bishop, Jenna L., Wickham, Mark E., Brown, Nathaniel Francis, Duong, Nancy, ...
Bacterial pathogens use horizontal gene transfer to acquire virulence factors that influence host colonization, alter virulence traits, and ultimately shape the outcome of disease following...
SopD acts cooperatively with SopB during Salmonella enterica serovar Typhimurium invasion (2007)
Bakowski, Malina A., Cirulis, Judith T., Brown, Nathaniel Francis, Finlay, B. Brett, Brumell, John H.
The intracellular bacterial pathogen, Salmonella enterica serovar Typhimurium (S. typhimurium), causes disease in a variety of hosts. To invade and replicate in host cells, these bacteria subvert...
Boyle, Erin C., Brown, Nathaniel Francis, Brumell, John H., Finlay, B. Brett
The ability of Salmonella enterica serovar Typhimurium (S. Typhimurium) to penetrate the intestinal epithelium is key to its pathogenesis. Bacterial invasion can be seen as a two-step process...
Virulence is positively selected by transmission success between mammalian hosts (2007)
Wickham, Mark E., Brown, Nathaniel Francis, Boyle, Erin C., Coombes, Brian K., Finlay, B. Brett
Virulence, defined as damage to the host, is a trait of pathogens that evolutionary theory suggests benefits the pathogen in the ''struggle for existence'' [1]. Pathogens employ virulence mechanisms...
Coombes, Brian K., Lowden, Michael J., Bishop, Jenna L., Wickham, Mark E., Brown, Nathaniel Francis, Duong, Nancy, ...
Bacterial pathogens use horizontal gene transfer to acquire virulence factors that influence host colonization, alter virulence traits, and ultimately shape the outcome of disease following...
SopD acts cooperatively with SopB during Salmonella enterica serovar Typhimurium invasion (2007)
Bakowski, Malina A., Cirulis, Judith T., Brown, Nathaniel Francis, Finlay, B. Brett, Brumell, John H.
The intracellular bacterial pathogen, Salmonella enterica serovar Typhimurium (S. typhimurium), causes disease in a variety of hosts. To invade and replicate in host cells, these bacteria subvert...
Boyle, Erin C., Brown, Nathaniel Francis, Brumell, John H., Finlay, B. Brett
The ability of Salmonella enterica serovar Typhimurium (S. Typhimurium) to penetrate the intestinal epithelium is key to its pathogenesis. Bacterial invasion can be seen as a two-step process...
Wickham, Mark E., Brown, Nathaniel Francis, Provias, John, Finlay, B. Brett, Coombes, Brian K.
BACKGROUND: Salmonella meningitis is a rare and serious infection of the central nervous system following acute Salmonella enterica sepsis. For this pathogen, no appropriate model has been reported...
Virulence is positively selected by transmission success between mammalian hosts (2007)
Wickham, Mark E., Brown, Nathaniel Francis, Boyle, Erin C., Coombes, Brian K., Finlay, B. Brett
Virulence, defined as damage to the host, is a trait of pathogens that evolutionary theory suggests benefits the pathogen in the ''struggle for existence'' [1]. Pathogens employ virulence mechanisms...
Coombes, Brian K., Lowden, Michael J., Bishop, Jenna L., Wickham, Mark E., Brown, Nathaniel Francis, Duong, Nancy, ...
Bacterial pathogens use horizontal gene transfer to acquire virulence factors that influence host colonization, alter virulence traits, and ultimately shape the outcome of disease following...
SopD acts cooperatively with SopB during Salmonella enterica serovar Typhimurium invasion (2007)
Bakowski, Malina A., Cirulis, Judith T., Brown, Nathaniel Francis, Finlay, B. Brett, Brumell, John H.
The intracellular bacterial pathogen, Salmonella enterica serovar Typhimurium (S. typhimurium), causes disease in a variety of hosts. To invade and replicate in host cells, these bacteria subvert...
Boyle, Erin C., Brown, Nathaniel Francis, Brumell, John H., Finlay, B. Brett
The ability of Salmonella enterica serovar Typhimurium (S. Typhimurium) to penetrate the intestinal epithelium is key to its pathogenesis. Bacterial invasion can be seen as a two-step process...
Crossing the Line: Selection and Evolution of Virulence Traits (2006)
Nat F. Brown, Mark E. Wickham, Brian K. Coombes, B. Brett Finlay
The evolution of pathogens presents a paradox. Pathogenic species are often absolutely dependent on their host species for their propagation through evolutionary time, yet the pathogenic lifestyle...
Boyle, Erin C., Brown, Nathaniel Francis, Finlay, B. Brett
Salmonella enterica serovar Typhimurium is a major cause of human gastroenteritis. Infection of epithelial monolayers by S. Typhimurium disrupts tight junctions that normally maintain the intestinal...
Brown, Nathaniel Francis, Szeto, Jason, Jiang, Xiuju, Coombes, Brian K., Finlay, B. Brett, Brumell, John H.
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen causing disease in several hosts. These bacteria use two distinct type III secretion systems that inject effector...
Wickham, Mark E., Lupp, Claudia, Mascarenhas, Mariola, Vazquez, Alejandra, Coombes, Brian K., Brown, Nathaniel Francis, ...
Although O157:H7 Shiga toxin–producing Escherichia coli (STEC) are the predominant cause of hemolytic-uremic syndrome (HUS) in the world, non-O157:H7 serotypes are a medically important cause of...
Boyle, Erin C., Brown, Nathaniel Francis, Finlay, B. Brett
Salmonella enterica serovar Typhimurium is a major cause of human gastroenteritis. Infection of epithelial monolayers by S. Typhimurium disrupts tight junctions that normally maintain the intestinal...
Brown, Nathaniel Francis, Szeto, Jason, Jiang, Xiuju, Coombes, Brian K., Finlay, B. Brett, Brumell, John H.
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen causing disease in several hosts. These bacteria use two distinct type III secretion systems that inject effector...
Wickham, Mark E., Lupp, Claudia, Mascarenhas, Mariola, Vazquez, Alejandra, Coombes, Brian K., Brown, Nathaniel Francis, ...
Although O157:H7 Shiga toxin–producing Escherichia coli (STEC) are the predominant cause of hemolytic-uremic syndrome (HUS) in the world, non-O157:H7 serotypes are a medically important cause of...
Boyle, Erin C., Brown, Nathaniel Francis, Finlay, B. Brett
Salmonella enterica serovar Typhimurium is a major cause of human gastroenteritis. Infection of epithelial monolayers by S. Typhimurium disrupts tight junctions that normally maintain the intestinal...
Brown, Nathaniel Francis, Szeto, Jason, Jiang, Xiuju, Coombes, Brian K., Finlay, B. Brett, Brumell, John H.
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen causing disease in several hosts. These bacteria use two distinct type III secretion systems that inject effector...
Wickham, Mark E., Lupp, Claudia, Mascarenhas, Mariola, Vazquez, Alejandra, Coombes, Brian K., Brown, Nathaniel Francis, ...
Although O157:H7 Shiga toxin–producing Escherichia coli (STEC) are the predominant cause of hemolytic-uremic syndrome (HUS) in the world, non-O157:H7 serotypes are a medically important cause of...
Boyle, Erin C., Brown, Nathaniel Francis, Finlay, B. Brett
Salmonella enterica serovar Typhimurium is a major cause of human gastroenteritis. Infection of epithelial monolayers by S. Typhimurium disrupts tight junctions that normally maintain the intestinal...
Brown, Nathaniel Francis, Szeto, Jason, Jiang, Xiuju, Coombes, Brian K., Finlay, B. Brett, Brumell, John H.
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen causing disease in several hosts. These bacteria use two distinct type III secretion systems that inject effector...
Wickham, Mark E., Lupp, Claudia, Mascarenhas, Mariola, Vazquez, Alejandra, Coombes, Brian K., Brown, Nathaniel Francis, ...
Although O157:H7 Shiga toxin–producing Escherichia coli (STEC) are the predominant cause of hemolytic-uremic syndrome (HUS) in the world, non-O157:H7 serotypes are a medically important cause of...
Evidence of a Large Novel Gene Pool Associated with Prokaryotic Genomic Islands (2005)
Korine Ung, Dana Aeschliman, Jenny Bryan, B. Brett Finlay
Microbial genes that are “novel” (no detectable homologs in other species) have become of increasing interest as environmental sampling suggests that there are many more such novel genes in...
Salmonella Pathogenicity Island 2 Is Expressed Prior to Penetrating the Intestine (2005)
Nat F. Brown, Bruce A. Vallance, Brian K. Coombes, Yanet Valdez, Bryan A. Coburn, B. Brett Finlay
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that causes disease in mice that resembles human typhoid. Typhoid pathogenesis consists of distinct phases in the...
Salmonella pathogenicity island 2 is expressed prior to penetrating the intestine (2005)
Brown, Nathaniel Francis, Vallance, Bruce A., Coombes, Brian K., Vandez, Yanet, Coburn, Bryan A., Finlay, B. Brett
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that causes disease in mice that resembles human typhoid. Typhoid pathogenesis consists of distinct phases in the...
Salmonella pathogenicity island 2 is expressed prior to penetrating the intestine (2005)
Brown, Nathaniel Francis, Vallance, Bruce A., Coombes, Brian K., Vandez, Yanet, Coburn, Bryan A., Finlay, B. Brett
Yes
Salmonella pathogenicity island 2 is expressed prior to penetrating the intestine (2005)
Brown, Nathaniel Francis, Vallance, Bruce A., Coombes, Brian K., Vandez, Yanet, Coburn, Bryan A., Finlay, B. Brett
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that causes disease in mice that resembles human typhoid. Typhoid pathogenesis consists of distinct phases in the...
Coombes, Brian K., Lowden, Michael J., Wickham, Mark E., Brown, Nathaniel Francis, Finlay, B. Brett
Short RNA regulatory molecules, microRNAs, and short interfering RNAs participate in a range of developmental gene networks by base-pairing with their target sequences. Consistent with these...
Coombes, Brian K., Wickham, Mark E., Brown, Nathaniel Francis, Lemire, Sebastien, Bossi, Lionello, Hsiao, William W. L., ...
Salmonella enterica serovar Typhimurium is lysogenized by several temperate bacteriophages that encode lysogenic conversion genes, which can act as virulence factors during infection and contribute...
Coombes, Brian K., Lowden, Michael J., Wickham, Mark E., Brown, Nathaniel Francis, Finlay, B. Brett
Short RNA regulatory molecules, microRNAs, and short interfering RNAs participate in a range of developmental gene networks by base-pairing with their target sequences. Consistent with these...
Coombes, Brian K., Wickham, Mark E., Brown, Nathaniel Francis, Lemire, Sebastien, Bossi, Lionello, Hsiao, William W. L., ...
Salmonella enterica serovar Typhimurium is lysogenized by several temperate bacteriophages that encode lysogenic conversion genes, which can act as virulence factors during infection and contribute...
Coombes, Brian K., Lowden, Michael J., Wickham, Mark E., Brown, Nathaniel Francis, Finlay, B. Brett
Short RNA regulatory molecules, microRNAs, and short interfering RNAs participate in a range of developmental gene networks by base-pairing with their target sequences. Consistent with these...
Coombes, Brian K., Wickham, Mark E., Brown, Nathaniel Francis, Lemire, Sebastien, Bossi, Lionello, Hsiao, William W. L., ...
Salmonella enterica serovar Typhimurium is lysogenized by several temperate bacteriophages that encode lysogenic conversion genes, which can act as virulence factors during infection and contribute...
Salmonella pathogenicity island 2 is expressed prior to penetrating the intestine (2005)
Brown, Nathaniel Francis, Vallance, Bruce A., Coombes, Brian K., Vandez, Yanet, Coburn, Bryan A., Finlay, B. Brett
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that causes disease in mice that resembles human typhoid. Typhoid pathogenesis consists of distinct phases in the...
Coombes, Brian K., Lowden, Michael J., Wickham, Mark E., Brown, Nathaniel Francis, Finlay, B. Brett
Short RNA regulatory molecules, microRNAs, and short interfering RNAs participate in a range of developmental gene networks by base-pairing with their target sequences. Consistent with these...
Coombes, Brian K., Wickham, Mark E., Brown, Nathaniel Francis, Lemire, Sebastien, Bossi, Lionello, Hsiao, William W. L., ...
Salmonella enterica serovar Typhimurium is lysogenized by several temperate bacteriophages that encode lysogenic conversion genes, which can act as virulence factors during infection and contribute...
Potter, Andrew A., Klashinsky, Sandra, Li, Yuling, Frey, Elizabeth, Townsend, Hugh, Rogan, Dragan, ...
Cattle are an important reservoir of Escherichia coli O157:H7 leading to contamination of food and water, and subsequent human disease. This pathogen colonizes its hosts by producing several proteins...
Coombes, Brian K., Brown, Nathaniel Francis, Vandez, Yanet, Brumell, John H., Finlay, B. Brett
Salmonella pathogenicity island (SPI)-2 is pivotal to the intracellular survival of Salmonella and for virulence in mammals. SPI-2 encodes virulence factors (called effectors) that are translocated...
Coombes, Brian K., Brown, Nathaniel Francis, Vandez, Yanet, Brumell, John H., Finlay, B. Brett
Salmonella pathogenicity island (SPI)-2 is pivotal to the intracellular survival of Salmonella and for virulence in mammals. SPI-2 encodes virulence factors(called effectors) that are translocated...
Coombes, Brian K., Brown, Nathaniel Francis, Vandez, Yanet, Brumell, John H., Finlay, B. Brett
Salmonella pathogenicity island (SPI)-2 is pivotal to the intracellular survival of Salmonella and for virulence in mammals. SPI-2 encodes virulence factors (called effectors) that are translocated...
Zaharik, Michelle L., Cullen, Vivian Li, Fung, Angela M., Libby, Stephen J., Kujat Choy, Sonya L., Coburn, Bryan, ...
Nramp1 is a transporter that pumps divalent cations from the vacuoles of phagocytic cells and is associated with the innate resistance of mice to diverse intracellular pathogens. We demonstrate that...
Maaser, Christian, Housley, Michael P., Iimura, Mitsutoshi, Smith, Jennifer R., Vallance, Bruce A., Finlay, B. Brett, ...
Citrobacter rodentium, a murine model pathogen for human enteropathogenic Escherichia coli, predominantly colonizes the lumen and mucosal surface of the colon and cecum and causes crypt hyperplasia...
Jiang, Xiuju, Rossanese, Olivia W., Brown, Nathaniel Francis, Kujat-Choy, Sonya, Galán, Jorge E., Finlay, B. Brett, ...
Salmonella resides within host cells in a vacuole that it modifies through the action of virulence proteins called effectors. Here we examined the role of two related effectors, SopD and SopD2, in...
Gruenheid, Samantha, Sekirov, Inna, Thomas, Nikhil A., Deng, Wanyin, O'Donnell, Paul, Goode, David, ...
Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 uses a specialized protein translocation apparatus, the type III secretion system (TTSS), to deliver bacterial effector proteins into host cells....
Jiang, Xiuju, Rossanese, Olivia W., Brown, Nathaniel Francis, Kujat-Choy, Sonya, Galán, Jorge E., Finlay, B. Brett, ...
Salmonella resides within host cells in a vacuole that it modifies through the action of virulence proteins called effectors. Here we examined the role of two related effectors, SopD and SopD2, in...
Gruenheid, Samantha, Sekirov, Inna, Thomas, Nikhil A., Deng, Wanyin, O'Donnell, Paul, Goode, David, ...
Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 uses a specialized protein translocation apparatus, the type III secretion system (TTSS), to deliver bacterial effector proteins into host cells....
Jiang, Xiuju, Rossanese, Olivia W., Brown, Nathaniel Francis, Kujat-Choy, Sonya, Galán, Jorge E., Finlay, B. Brett, ...
Salmonella resides within host cells in a vacuole that it modifies through the action of virulence proteins called effectors. Here we examined the role of two related effectors, SopD and SopD2, in...
Gruenheid, Samantha, Sekirov, Inna, Thomas, Nikhil A., Deng, Wanyin, O'Donnell, Paul, Goode, David, ...
Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 uses a specialized protein translocation apparatus, the type III secretion system (TTSS), to deliver bacterial effector proteins into host cells....
Xiuju Jiang, Olivia W. Rossanese, Nat F. Brown, Sonya Kujat-choy, Jorge E. Galán, B. Brett Finlay, ...
et al. The related effector proteins SopD and SopD2 from Salmonella enterica serovar Typhimurium contribute to virulence during systemic infection of mice
Coombes, Brian K., Brown, Nathaniel Francis, Vandez, Yanet, Brumell, John H., Finlay, B. Brett
Salmonella pathogenicity island (SPI)-2 is pivotal to the intracellular survival of Salmonella and for virulence in mammals. SPI-2 encodes virulence factors (called effectors) that are translocated...
Jiang, Xiuju, Rossanese, Olivia W., Brown, Nathaniel Francis, Kujat-Choy, Sonya, Galán, Jorge E., Finlay, B. Brett, ...
Salmonella resides within host cells in a vacuole that it modifies through the action of virulence proteins called effectors. Here we examined the role of two related effectors, SopD and SopD2, in...
Gruenheid, Samantha, Sekirov, Inna, Thomas, Nikhil A., Deng, Wanyin, O'Donnell, Paul, Goode, David, ...
Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 uses a specialized protein translocation apparatus, the type III secretion system (TTSS), to deliver bacterial effector proteins into host cells....
Coombes, Brian K., Brown, Nathaniel Francis, Kujat-Choy, Sonya, Vallance, Bruce A., Finlay, B. Brett
The Salmonella pathogenicity island-2 (SPI2) is a virulence locus on the bacterial chromosome required for intracellular proliferation and systemic infection in mice. Cell culture models and a murine...
Brumell, John H., Kujat-Choy, Sonya, Brown, Nathaniel Francis, Vallance, Bruce A., Knodler, Leigh A., Finlay, B. Brett
Salmonella typhimurium is a facultative intracellular pathogen that utilizes two type III secretion systems to deliver virulence proteins into host cells. These proteins, termed effectors, alter host...
Coombes, Brian K., Brown, Nathaniel Francis, Kujat-Choy, Sonya, Vallance, Bruce A., Finlay, B. Brett
The Salmonella pathogenicity island-2 (SPI2) is a virulence locus on the bacterial chromosome required for intracellular proliferation and systemic infection in mice. Cell culture models and a murine...
Brumell, John H., Kujat-Choy, Sonya, Brown, Nathaniel Francis, Vallance, Bruce A., Knodler, Leigh A., Finlay, B. Brett
Salmonella typhimurium is a facultative intracellular pathogen that utilizes two type III secretion systems to deliver virulence proteins into host cells. These proteins, termed effectors, alter host...
Coombes, Brian K., Brown, Nathaniel Francis, Kujat-Choy, Sonya, Vallance, Bruce A., Finlay, B. Brett
The Salmonella pathogenicity island-2 (SPI2) is a virulence locus on the bacterial chromosome required for intracellular proliferation and systemic infection in mice. Cell culture models and a murine...
Brumell, John H., Kujat-Choy, Sonya, Brown, Nathaniel Francis, Vallance, Bruce A., Knodler, Leigh A., Finlay, B. Brett
Salmonella typhimurium is a facultative intracellular pathogen that utilizes two type III secretion systems to deliver virulence proteins into host cells. These proteins, termed effectors, alter host...
Coombes, Brian K., Brown, Nathaniel Francis, Kujat-Choy, Sonya, Vallance, Bruce A., Finlay, B. Brett
The Salmonella pathogenicity island-2 (SPI2) is a virulence locus on the bacterial chromosome required for intracellular proliferation and systemic infection in mice. Cell culture models and a murine...
Brumell, John H., Kujat-Choy, Sonya, Brown, Nathaniel Francis, Vallance, Bruce A., Knodler, Leigh A., Finlay, B. Brett
Salmonella typhimurium is a facultative intracellular pathogen that utilizes two type III secretion systems to deliver virulence proteins into host cells. These proteins, termed effectors, alter host...
Brinkman, Fiona S.L., Blanchard, Jeffrey L., Cherkasov, Artem, Av-Gay, Yossef, Brunham, Robert C., Fernandez, Rachel C., ...
Brinkman, Fiona S.L., Blanchard, Jeffrey L., Cherkasov, Artem, Av-Gay, Yossef, Brunham, Robert C., Fernandez, Rachel C., ...
Introduction: microbiology and immunology: lessons learned from Salmonella (2001)
Kaufmann,Stefan H. E., Raupach,Barbel, Finlay,B. Brett
Salmonella enterica, a Gram-negative bacterium, causes significant morbidity and mortality worldwide, and is an excellent model to study bacterial pathogenesis and cellular immune responses. With the...
Studies of transfer (tra) genes from IncF plasmids / (1986)
Finlay, B. Brett (Barton Brett)
Submitted to the Faculty of Graduate Studies and Research in partial fulfilment of the requirements for the degree of Doctor of Philosophy, Department of Biochemistry.
Mills, Scott D., Boland, Anne, Sory, Marie-Paule, Van Der Smissen, Patrick, Kerbourch, Corinne, Finlay, B. Brett, ...
Yersiniae, causative agents of plague and gastrointestinal diseases, secrete and translocate Yop effector proteins into the cytosol of macrophages, leading to disruption of host defense mechanisms....
Cordeiro, Carol, Wiseman, David J., Lutwyche, Peter, Uh, Mitchell, Evans, Jennifer C., Finlay, B. Brett, ...
Encapsulation of gentamicin in liposomes can be used to achieve intracellular delivery and broaden the clinical utility of this drug. We have previously described a novel, rationally designed,...
Enteropathogenic Escherichia coli Inhibits Phagocytosis
Goosney, Danika L., Celli, Jean, Kenny, Brendan, Finlay, B. Brett
Enteropathogenic Escherichia coli (EPEC) interacts with intestinal epithelial cells, activating host signaling pathways leading to cytoskeletal rearrangements and ultimately diarrhea. In this study,...
Type III Secretion-Dependent Hemolytic Activity of Enteropathogenic Escherichia coli
Warawa, Jonathan, Finlay, B. Brett, Kenny, Brendan
Enteropathogenic Escherichia coli (EPEC) was found to exhibit a type III secretion-dependent, contact-mediated, hemolytic activity requiring the EspA, EspB, and EspD secreted proteins. EspB and EspD...
Pfeifer, Cheryl G., Marcus, Sandra L., Steele-Mortimer, Olivia, Knodler, Leigh A., Finlay, B. Brett
Survival and growth of salmonellae within host cells are important aspects of bacterial virulence. We have developed an assay to identify Salmonella typhimurium genes that are induced inside...
Role of EspB in Experimental Human Enteropathogenic Escherichia coli Infection
Tacket, Carol O., Sztein, Marcelo B., Losonsky, Genevieve, Abe, Akio, Finlay, B. Brett, McNamara, Barry P., ...
Enteropathogenic Escherichia coli (EPEC), a leading cause of diarrhea among infants in developing countries, induces dramatic alterations in host cell architecture that depend on a type III secretion...
Devinney, Rebekah, Nisan, Israel, Ruschkowski, Sharon, Rosenshine, Ilan, Finlay, B. Brett
Enteropathogenic Escherichia coli (EPEC) strain 30-5-1(3) has been reported to form attaching and effacing (A/E) lesions without Tir tyrosine phosphorylation. In this study, we show that 30-5-1(3),...
Goosney, Danika L., DeVinney, Rebekah, Finlay, B. Brett
Enteropathogenic Escherichia coli (EPEC) is a human pathogen that attaches to intestinal epithelial cells and causes chronic watery diarrhea. A close relative, enterohemorrhagic E. coli (EHEC),...
Heczko, Ursula, Carthy, Chris M., O'Brien, Bronwyn A., Finlay, B. Brett
Significant changes occur in intestinal epithelial cells after infection with enteropathogenic Escherichia coli (EPEC). However, it is unclear whether this pathogen alters rates of apoptosis. By...
De Grado, Myriam, Rosenberger, Carrie M., Gauthier, Annick, Vallance, Bruce A., Finlay, B. Brett
Enteropathogenic Escherichia coli (EPEC) is an extracellular bacterial pathogen that infects the human intestinal epithelium and is a major cause of infantile diarrhea in developing countries. EPEC...
Deng, Wanyin, Li, Yuling, Vallance, Bruce A., Finlay, B. Brett
The family of attaching and effacing (A/E) bacterial pathogens, which includes diarrheagenic enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC), remains a significant...
Human Response to Escherichia coli O157:H7 Infection: Antibodies to Secreted Virulence Factors
Li, Yuling, Frey, Elizabeth, Mackenzie, Andrew M. R., Finlay, B. Brett
Vaccination has been proposed for the prevention of disease due to enterohemorrhagic Escherichia coli (EHEC), but the immune response following human infection, including the choice of potential...
Gauthier, Annick, De Grado, Myriam, Finlay, B. Brett
Enteropathogenic Escherichia coli (EPEC) inserts its receptor for intimate adherence (Tir) into host cell membranes by using a type III secretion system. Detergents are frequently used to fractionate...
Steele-Mortimer, Olivia, St-Louis, Maryse, Olivier, Martin, Finlay, B. Brett
Phagosome acidification is an important component of the microbicidal response by infected eukaryotic cells. Thus, intracellular pathogens that reside within phagosomes must either block phagosome...
Lutwyche, Peter, Cordeiro, Carol, Wiseman, David J., St-Louis, Maryse, Uh, Mitchell, Hope, Michael J., ...
Cell membranes are relatively impermeable to the antibiotic gentamicin, a factor that, along with the toxicity of gentamicin, precludes its use against many important intracellular bacterial...
Tang, Patrick, Sutherland, Claire L., Gold, Michael R., Finlay, B. Brett
PD98059, a specific inhibitor of MEK-1 mitogen-activated protein (MAP) kinase kinase, blocked Listeria monocytogenes invasion into HeLa epithelial cells. The effects of PD98059 were reversible, as...
Mills, Scott D., Ruschkowski, Sharon R., Stein, Murry A., Finlay, B. Brett
Outer membrane porin genes of Salmonella typhimurium, including ompC, ompF, and tppB, are regulated by the products of ompB, a two-component regulatory locus encoding OmpR and EnvZ. S. typhimurium...
Wilson, Dan R., Siebers, Annette, Finlay, B. Brett
Although substantial advancements have been made in the development of efficacious acellular vaccines against Bordetella pertussis, continued progress requires better understanding of the antigenic...
Functional Expression of Nramp1 In Vitro in the Murine Macrophage Line RAW264.7
Govoni, Gregory, Canonne-Hergaux, François, Pfeifer, Cheryl G., Marcus, Sandra L., Mills, Scott D., Hackam, David J., ...
Mutations at the Nramp1 locus in vivo cause susceptibility to infection by unrelated intracellular microbes. Nramp1 encodes an integral membrane protein abundantly expressed in the...
DeVinney, Rebekah, Stein, Markus, Reinscheid, Dieter, Abe, Akio, Ruschkowski, Sharon, Finlay, B. Brett
Intimate attachment to the host cell leading to the formation of attaching and effacing (A/E) lesions is an essential feature of enterohemorrhagic Escherichia coli (EHEC) O157:H7 pathogenesis. In a...
Vallance, Bruce A., Deng, Wanyin, Knodler, Leigh A., Finlay, B. Brett
The bacterial pathogen Citrobacter rodentium belongs to a family of gastrointestinal pathogens that includes enteropathogenic and enterohemorrhagic Escherichia coli and is the causative agent of...
Brumell, John H., Tang, Patrick, Zaharik, Michelle L., Finlay, B. Brett
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that inhabits a vacuolar compartment, called the Salmonella-containing vacuole (SCV), in infected host cells....
Vallance, Bruce A., Deng, Wanyin, De Grado, Myriam, Chan, Crystal, Jacobson, Kevan, Finlay, B. Brett
Citrobacter rodentium belongs to the attaching and effacing family of enteric bacterial pathogens that includes both enteropathogenic and enterohemorrhagic Escherichia coli. These bacteria infect...
Zaharik, Michelle L., Vallance, Bruce A., Puente, José L., Gros, Philippe, Finlay, B. Brett
Nramp1 (Natural resistance-associated macrophage protein-1; also known as Slc11a1) is a host resistance gene that provides protection against several intracellular pathogens, including Salmonella...
Celli, Jean, Olivier, Martin, Finlay, B.Brett
The extracellular pathogen enteropathogenic Escherichia coli (EPEC) uses a type III secretion system to inhibit its uptake by macrophages. We show that EPEC antiphagocytosis is independent of the...
Kurz, C.Léopold, Chauvet, Sophie, Andrès, Emmanuel, Aurouze, Marianne, Vallet, Isabelle, Michel, Gérard P.F., ...
The human opportunistic pathogen Serratia marcescens is a bacterium with a broad host range, and represents a growing problem for public health. Serratia marcescens kills Caenorhabditis elegans after...
Host Susceptibility to the Attaching and Effacing Bacterial Pathogen Citrobacter rodentium
Vallance, Bruce A., Deng, Wanyin, Jacobson, Kevan, Finlay, B. Brett
Many studies have shown that genetic susceptibility plays a key role in determining whether bacterial pathogens successfully infect and cause disease in potential hosts. Surprisingly, whether host...
Gauthier, Annick, Puente, Jose Luis, Finlay, B. Brett
At least 16 proteins are thought to be involved in forming the enteropathogenic Escherichia coli (EPEC) type III translocation apparatus which delivers virulence factors into host cells, yet their...
Brinkman, Fiona S.L., Blanchard, Jeffrey L., Cherkasov, Artem, Av-Gay, Yossef, Brunham, Robert C., Fernandez, Rachel C., ...
An unusually high proportion of proteins encoded in Chlamydia genomes are most similar to plant proteins, leading to proposals that a Chlamydia ancestor obtained genes from a plant or plant-like host...
Gauthier, Annick, Finlay, B. Brett
Few interactions have been reported between effectors and components of the type III secretion apparatus, although many interactions have been demonstrated between type III effectors and their...
Rosenberger, Carrie M., Gallo, Richard L., Finlay, B. Brett
Antimicrobial peptides have established an important role in the defense against extracellular infections, but the expression of cationic peptides within macrophages as an antibacterial effector...
Dissecting virulence: Systematic and functional analyses of a pathogenicity island
Deng, Wanyin, Puente, José L., Gruenheid, Samantha, Li, Yuling, Vallance, Bruce A., Vázquez, Alejandra, ...
Bacterial pathogenicity islands (PAI) often encode both effector molecules responsible for disease and secretion systems that deliver these effectors to host cells. Human enterohemorrhagic...
Maaser, Christian, Housley, Michael P., Iimura, Mitsutoshi, Smith, Jennifer R., Vallance, Bruce A., Finlay, B. Brett, ...
Citrobacter rodentium, a murine model pathogen for human enteropathogenic Escherichia coli, predominantly colonizes the lumen and mucosal surface of the colon and cecum and causes crypt hyperplasia...
Salmonella Impairs RILP Recruitment to Rab7 during Maturation of Invasion Vacuoles
Harrison, Rene E., Brumell, John H., Khandani, Arian, Bucci, Cecilia, Scott, Cameron C., Jiang, Xiuju, ...
After invasion of epithelial cells, Salmonella enterica Typhimurium resides within membrane-bound vacuoles where it survives and replicates. Like endocytic vesicles, the Salmonella-containing...
SseK1 and SseK2 Are Novel Translocated Proteins of Salmonella enterica Serovar Typhimurium
Kujat Choy, Sonya L., Boyle, Erin C., Gal-Mor, Ohad, Goode, David L., Valdez, Yanet, Vallance, Bruce A., ...
Salmonella enterica is a gram-negative, facultative intracellular pathogen that causes disease symptoms ranging from gastroenteritis to typhoid fever. A key virulence strategy is the translocation of...
Zaharik, Michelle L., Cullen, Vivian Li, Fung, Angela M., Libby, Stephen J., Kujat Choy, Sonya L., Coburn, Bryan, ...
Nramp1 is a transporter that pumps divalent cations from the vacuoles of phagocytic cells and is associated with the innate resistance of mice to diverse intracellular pathogens. We demonstrate that...
Conlin, Victoria S., Curtis, Susan B., Zhao, Ying, Moore, Edwin D. W., Smith, Valerie C., Meloche, R. Mark, ...
The human gastric pathogen Helicobacter pylori attaches to antral epithelial cells in vivo. Cultured human antral epithelial cells, AGS and NCI-N87 cell lines, were grown in the absence or presence...
Hardwidge, Philip R., Deng, Wanyin, Vallance, Bruce A., Rodriguez-Escudero, Isabel, Cid, Victor J., Molina, Maria, ...
EspG is a conserved protein encoded by the locus of enterocyte effacement (LEE) of attaching and effacing (A/E) pathogens, including enteropathogenic and enterohemorrhagic Escherichia coli and...
Deng, Wanyin, Li, Yuling, Hardwidge, Philip R., Frey, Elizabeth A., Pfuetzner, Richard A., Lee, Sansan, ...
Human enteropathogenic Escherichia coli (EPEC), enterohemorrhagic E. coli (EHEC), and the mouse pathogen Citrobacter rodentium (CR) belong to the family of attaching and effacing (A/E) bacterial...
Coburn, Bryan, Li, Yuling, Owen, David, Vallance, Bruce A., Finlay, B. Brett
Salmonella species cause a wide range of disease in multiple hosts. Salmonella enterica serovar Typhimurium causes self-limited intestinal disease in humans and systemic typhoid-like illness in...
Transcriptional Inhibitor of Virulence Factors in Enteropathogenic Escherichia coli
Gauthier, Annick, Robertson, Marilyn L., Lowden, Michael, Ibarra, J. Antonio, Puente, José Luis, Finlay, B. Brett
The type III secretion system (TTSS) is a key virulence mechanism of many important gram-negative bacterial pathogens. The TTSS is conserved among different bacterial pathogens, and mutations and...
Coombes, Brian K., Coburn, Bryan A., Potter, Andrew A., Gomis, Susantha, Mirakhur, Kuldip, Li, Yuling, ...
We have developed a novel ileal loop model for use in calves to analyze the contribution of Salmonella enterica serovar Typhimurium type III secretion systems to disease processes in vivo. Our model...
Evidence of a Large Novel Gene Pool Associated with Prokaryotic Genomic Islands
Hsiao, William W. L, Ung, Korine, Aeschliman, Dana, Bryan, Jenny, Finlay, B. Brett, Brinkman, Fiona S. L
Microbial genes that are “novel” (no detectable homologs in other species) have become of increasing interest as environmental sampling suggests that there are many more such novel genes in...
Salmonella Pathogenicity Island 2 Is Expressed Prior to Penetrating the Intestine
Brown, Nat F, Vallance, Bruce A, Coombes, Brian K, Valdez, Yanet, Coburn, Bryan A, Finlay, B. Brett
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that causes disease in mice that resembles human typhoid. Typhoid pathogenesis consists of distinct phases in the...
Barba, Jeannette, Bustamante, Víctor H., Flores-Valdez, Mario A., Deng, Wanyin, Finlay, B. Brett, Puente, José L.
The formation of attaching and effacing (A/E) lesions on intestinal epithelial cells is an essential step in the pathogenesis of human enteropathogenic and enterohemorrhagic Escherichia coli and of...
Martin-Orozco, Natalia, Touret, Nicolas, Zaharik, Michael L., Park, Edwin, Kopelman, Raoul, Miller, Samuel, ...
The objective of these studies was to analyze the role of the ionic environment of phagosomal vacuoles in the control of pathogens by macrophages. Digital imaging and flow cytometry were used to...
Crossing the Line: Selection and Evolution of Virulence Traits
Brown, Nat F, Wickham, Mark E, Coombes, Brian K, Finlay, B. Brett
The evolution of pathogens presents a paradox. Pathogenic species are often absolutely dependent on their host species for their propagation through evolutionary time, yet the pathogenic lifestyle...
Coombes, Brian K., Wickham, Mark E., Lowden, Michael J., Brown, Nat F., Finlay, B. Brett
Salmonella enterica relies on a type III secretion system encoded in Salmonella pathogenicity island-2 (SPI-2) to survive and replicate within macrophages at systemic sites during typhoid. SPI-2...
Khan, Mohammed A., Ma, Caixia, Knodler, Leigh A., Valdez, Yanet, Rosenberger, Carrie M., Deng, Wanyin, ...
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) are noninvasive bacterial pathogens that infect their hosts' intestinal epithelium, causing severe diarrheal disease....
Mills, Scott D., Boland, Anne, Sory, Marie-Paule, Van Der Smissen, Patrick, Kerbourch, Corinne, Finlay, B. Brett, ...
Yersiniae, causative agents of plague and gastrointestinal diseases, secrete and translocate Yop effector proteins into the cytosol of macrophages, leading to disruption of host defense mechanisms....
Cordeiro, Carol, Wiseman, David J., Lutwyche, Peter, Uh, Mitchell, Evans, Jennifer C., Finlay, B. Brett, ...
Encapsulation of gentamicin in liposomes can be used to achieve intracellular delivery and broaden the clinical utility of this drug. We have previously described a novel, rationally designed,...
Enteropathogenic Escherichia coli Inhibits Phagocytosis
Goosney, Danika L., Celli, Jean, Kenny, Brendan, Finlay, B. Brett
Enteropathogenic Escherichia coli (EPEC) interacts with intestinal epithelial cells, activating host signaling pathways leading to cytoskeletal rearrangements and ultimately diarrhea. In this study,...
Type III Secretion-Dependent Hemolytic Activity of Enteropathogenic Escherichia coli
Warawa, Jonathan, Finlay, B. Brett, Kenny, Brendan
Enteropathogenic Escherichia coli (EPEC) was found to exhibit a type III secretion-dependent, contact-mediated, hemolytic activity requiring the EspA, EspB, and EspD secreted proteins. EspB and EspD...
Pfeifer, Cheryl G., Marcus, Sandra L., Steele-Mortimer, Olivia, Knodler, Leigh A., Finlay, B. Brett
Survival and growth of salmonellae within host cells are important aspects of bacterial virulence. We have developed an assay to identify Salmonella typhimurium genes that are induced inside...
Role of EspB in Experimental Human Enteropathogenic Escherichia coli Infection
Tacket, Carol O., Sztein, Marcelo B., Losonsky, Genevieve, Abe, Akio, Finlay, B. Brett, McNamara, Barry P., ...
Enteropathogenic Escherichia coli (EPEC), a leading cause of diarrhea among infants in developing countries, induces dramatic alterations in host cell architecture that depend on a type III secretion...
Devinney, Rebekah, Nisan, Israel, Ruschkowski, Sharon, Rosenshine, Ilan, Finlay, B. Brett
Enteropathogenic Escherichia coli (EPEC) strain 30-5-1(3) has been reported to form attaching and effacing (A/E) lesions without Tir tyrosine phosphorylation. In this study, we show that 30-5-1(3),...
Goosney, Danika L., DeVinney, Rebekah, Finlay, B. Brett
Enteropathogenic Escherichia coli (EPEC) is a human pathogen that attaches to intestinal epithelial cells and causes chronic watery diarrhea. A close relative, enterohemorrhagic E. coli (EHEC),...
Heczko, Ursula, Carthy, Chris M., O'Brien, Bronwyn A., Finlay, B. Brett
Significant changes occur in intestinal epithelial cells after infection with enteropathogenic Escherichia coli (EPEC). However, it is unclear whether this pathogen alters rates of apoptosis. By...
De Grado, Myriam, Rosenberger, Carrie M., Gauthier, Annick, Vallance, Bruce A., Finlay, B. Brett
Enteropathogenic Escherichia coli (EPEC) is an extracellular bacterial pathogen that infects the human intestinal epithelium and is a major cause of infantile diarrhea in developing countries. EPEC...
Deng, Wanyin, Li, Yuling, Vallance, Bruce A., Finlay, B. Brett
The family of attaching and effacing (A/E) bacterial pathogens, which includes diarrheagenic enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC), remains a significant...
Human Response to Escherichia coli O157:H7 Infection: Antibodies to Secreted Virulence Factors
Li, Yuling, Frey, Elizabeth, Mackenzie, Andrew M. R., Finlay, B. Brett
Vaccination has been proposed for the prevention of disease due to enterohemorrhagic Escherichia coli (EHEC), but the immune response following human infection, including the choice of potential...
Gauthier, Annick, De Grado, Myriam, Finlay, B. Brett
Enteropathogenic Escherichia coli (EPEC) inserts its receptor for intimate adherence (Tir) into host cell membranes by using a type III secretion system. Detergents are frequently used to fractionate...
Steele-Mortimer, Olivia, St-Louis, Maryse, Olivier, Martin, Finlay, B. Brett
Phagosome acidification is an important component of the microbicidal response by infected eukaryotic cells. Thus, intracellular pathogens that reside within phagosomes must either block phagosome...
Lutwyche, Peter, Cordeiro, Carol, Wiseman, David J., St-Louis, Maryse, Uh, Mitchell, Hope, Michael J., ...
Cell membranes are relatively impermeable to the antibiotic gentamicin, a factor that, along with the toxicity of gentamicin, precludes its use against many important intracellular bacterial...
Tang, Patrick, Sutherland, Claire L., Gold, Michael R., Finlay, B. Brett
PD98059, a specific inhibitor of MEK-1 mitogen-activated protein (MAP) kinase kinase, blocked Listeria monocytogenes invasion into HeLa epithelial cells. The effects of PD98059 were reversible, as...
Mills, Scott D., Ruschkowski, Sharon R., Stein, Murry A., Finlay, B. Brett
Outer membrane porin genes of Salmonella typhimurium, including ompC, ompF, and tppB, are regulated by the products of ompB, a two-component regulatory locus encoding OmpR and EnvZ. S. typhimurium...
Wilson, Dan R., Siebers, Annette, Finlay, B. Brett
Although substantial advancements have been made in the development of efficacious acellular vaccines against Bordetella pertussis, continued progress requires better understanding of the antigenic...
Functional Expression of Nramp1 In Vitro in the Murine Macrophage Line RAW264.7
Govoni, Gregory, Canonne-Hergaux, François, Pfeifer, Cheryl G., Marcus, Sandra L., Mills, Scott D., Hackam, David J., ...
Mutations at the Nramp1 locus in vivo cause susceptibility to infection by unrelated intracellular microbes. Nramp1 encodes an integral membrane protein abundantly expressed in the...
DeVinney, Rebekah, Stein, Markus, Reinscheid, Dieter, Abe, Akio, Ruschkowski, Sharon, Finlay, B. Brett
Intimate attachment to the host cell leading to the formation of attaching and effacing (A/E) lesions is an essential feature of enterohemorrhagic Escherichia coli (EHEC) O157:H7 pathogenesis. In a...
Vallance, Bruce A., Deng, Wanyin, Knodler, Leigh A., Finlay, B. Brett
The bacterial pathogen Citrobacter rodentium belongs to a family of gastrointestinal pathogens that includes enteropathogenic and enterohemorrhagic Escherichia coli and is the causative agent of...
Brumell, John H., Tang, Patrick, Zaharik, Michelle L., Finlay, B. Brett
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that inhabits a vacuolar compartment, called the Salmonella-containing vacuole (SCV), in infected host cells....
Vallance, Bruce A., Deng, Wanyin, De Grado, Myriam, Chan, Crystal, Jacobson, Kevan, Finlay, B. Brett
Citrobacter rodentium belongs to the attaching and effacing family of enteric bacterial pathogens that includes both enteropathogenic and enterohemorrhagic Escherichia coli. These bacteria infect...
Zaharik, Michelle L., Vallance, Bruce A., Puente, José L., Gros, Philippe, Finlay, B. Brett
Nramp1 (Natural resistance-associated macrophage protein-1; also known as Slc11a1) is a host resistance gene that provides protection against several intracellular pathogens, including Salmonella...
Celli, Jean, Olivier, Martin, Finlay, B.Brett
The extracellular pathogen enteropathogenic Escherichia coli (EPEC) uses a type III secretion system to inhibit its uptake by macrophages. We show that EPEC antiphagocytosis is independent of the...
Kurz, C.Léopold, Chauvet, Sophie, Andrès, Emmanuel, Aurouze, Marianne, Vallet, Isabelle, Michel, Gérard P.F., ...
The human opportunistic pathogen Serratia marcescens is a bacterium with a broad host range, and represents a growing problem for public health. Serratia marcescens kills Caenorhabditis elegans after...
Host Susceptibility to the Attaching and Effacing Bacterial Pathogen Citrobacter rodentium
Vallance, Bruce A., Deng, Wanyin, Jacobson, Kevan, Finlay, B. Brett
Many studies have shown that genetic susceptibility plays a key role in determining whether bacterial pathogens successfully infect and cause disease in potential hosts. Surprisingly, whether host...
Gauthier, Annick, Puente, Jose Luis, Finlay, B. Brett
At least 16 proteins are thought to be involved in forming the enteropathogenic Escherichia coli (EPEC) type III translocation apparatus which delivers virulence factors into host cells, yet their...
Brinkman, Fiona S.L., Blanchard, Jeffrey L., Cherkasov, Artem, Av-Gay, Yossef, Brunham, Robert C., Fernandez, Rachel C., ...
An unusually high proportion of proteins encoded in Chlamydia genomes are most similar to plant proteins, leading to proposals that a Chlamydia ancestor obtained genes from a plant or plant-like host...
Gauthier, Annick, Finlay, B. Brett
Few interactions have been reported between effectors and components of the type III secretion apparatus, although many interactions have been demonstrated between type III effectors and their...
Rosenberger, Carrie M., Gallo, Richard L., Finlay, B. Brett
Antimicrobial peptides have established an important role in the defense against extracellular infections, but the expression of cationic peptides within macrophages as an antibacterial effector...
Dissecting virulence: Systematic and functional analyses of a pathogenicity island
Deng, Wanyin, Puente, José L., Gruenheid, Samantha, Li, Yuling, Vallance, Bruce A., Vázquez, Alejandra, ...
Bacterial pathogenicity islands (PAI) often encode both effector molecules responsible for disease and secretion systems that deliver these effectors to host cells. Human enterohemorrhagic...
Maaser, Christian, Housley, Michael P., Iimura, Mitsutoshi, Smith, Jennifer R., Vallance, Bruce A., Finlay, B. Brett, ...
Citrobacter rodentium, a murine model pathogen for human enteropathogenic Escherichia coli, predominantly colonizes the lumen and mucosal surface of the colon and cecum and causes crypt hyperplasia...
Salmonella Impairs RILP Recruitment to Rab7 during Maturation of Invasion Vacuoles
Harrison, Rene E., Brumell, John H., Khandani, Arian, Bucci, Cecilia, Scott, Cameron C., Jiang, Xiuju, ...
After invasion of epithelial cells, Salmonella enterica Typhimurium resides within membrane-bound vacuoles where it survives and replicates. Like endocytic vesicles, the Salmonella-containing...
SseK1 and SseK2 Are Novel Translocated Proteins of Salmonella enterica Serovar Typhimurium
Kujat Choy, Sonya L., Boyle, Erin C., Gal-Mor, Ohad, Goode, David L., Valdez, Yanet, Vallance, Bruce A., ...
Salmonella enterica is a gram-negative, facultative intracellular pathogen that causes disease symptoms ranging from gastroenteritis to typhoid fever. A key virulence strategy is the translocation of...
Zaharik, Michelle L., Cullen, Vivian Li, Fung, Angela M., Libby, Stephen J., Kujat Choy, Sonya L., Coburn, Bryan, ...
Nramp1 is a transporter that pumps divalent cations from the vacuoles of phagocytic cells and is associated with the innate resistance of mice to diverse intracellular pathogens. We demonstrate that...
Conlin, Victoria S., Curtis, Susan B., Zhao, Ying, Moore, Edwin D. W., Smith, Valerie C., Meloche, R. Mark, ...
The human gastric pathogen Helicobacter pylori attaches to antral epithelial cells in vivo. Cultured human antral epithelial cells, AGS and NCI-N87 cell lines, were grown in the absence or presence...
Hardwidge, Philip R., Deng, Wanyin, Vallance, Bruce A., Rodriguez-Escudero, Isabel, Cid, Victor J., Molina, Maria, ...
EspG is a conserved protein encoded by the locus of enterocyte effacement (LEE) of attaching and effacing (A/E) pathogens, including enteropathogenic and enterohemorrhagic Escherichia coli and...
Deng, Wanyin, Li, Yuling, Hardwidge, Philip R., Frey, Elizabeth A., Pfuetzner, Richard A., Lee, Sansan, ...
Human enteropathogenic Escherichia coli (EPEC), enterohemorrhagic E. coli (EHEC), and the mouse pathogen Citrobacter rodentium (CR) belong to the family of attaching and effacing (A/E) bacterial...
Coburn, Bryan, Li, Yuling, Owen, David, Vallance, Bruce A., Finlay, B. Brett
Salmonella species cause a wide range of disease in multiple hosts. Salmonella enterica serovar Typhimurium causes self-limited intestinal disease in humans and systemic typhoid-like illness in...
Transcriptional Inhibitor of Virulence Factors in Enteropathogenic Escherichia coli
Gauthier, Annick, Robertson, Marilyn L., Lowden, Michael, Ibarra, J. Antonio, Puente, José Luis, Finlay, B. Brett
The type III secretion system (TTSS) is a key virulence mechanism of many important gram-negative bacterial pathogens. The TTSS is conserved among different bacterial pathogens, and mutations and...
Coombes, Brian K., Coburn, Bryan A., Potter, Andrew A., Gomis, Susantha, Mirakhur, Kuldip, Li, Yuling, ...
We have developed a novel ileal loop model for use in calves to analyze the contribution of Salmonella enterica serovar Typhimurium type III secretion systems to disease processes in vivo. Our model...
Evidence of a Large Novel Gene Pool Associated with Prokaryotic Genomic Islands
Hsiao, William W. L, Ung, Korine, Aeschliman, Dana, Bryan, Jenny, Finlay, B. Brett, Brinkman, Fiona S. L
Microbial genes that are “novel” (no detectable homologs in other species) have become of increasing interest as environmental sampling suggests that there are many more such novel genes in...
Salmonella Pathogenicity Island 2 Is Expressed Prior to Penetrating the Intestine
Brown, Nat F, Vallance, Bruce A, Coombes, Brian K, Valdez, Yanet, Coburn, Bryan A, Finlay, B. Brett
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that causes disease in mice that resembles human typhoid. Typhoid pathogenesis consists of distinct phases in the...
Barba, Jeannette, Bustamante, Víctor H., Flores-Valdez, Mario A., Deng, Wanyin, Finlay, B. Brett, Puente, José L.
The formation of attaching and effacing (A/E) lesions on intestinal epithelial cells is an essential step in the pathogenesis of human enteropathogenic and enterohemorrhagic Escherichia coli and of...
Coombes, Brian K., Wickham, Mark E., Lowden, Michael J., Brown, Nat F., Finlay, B. Brett
Salmonella enterica relies on a type III secretion system encoded in Salmonella pathogenicity island-2 (SPI-2) to survive and replicate within macrophages at systemic sites during typhoid. SPI-2...
Martin-Orozco, Natalia, Touret, Nicolas, Zaharik, Michael L., Park, Edwin, Kopelman, Raoul, Miller, Samuel, ...
The objective of these studies was to analyze the role of the ionic environment of phagosomal vacuoles in the control of pathogens by macrophages. Digital imaging and flow cytometry were used to...
Khan, Mohammed A., Ma, Caixia, Knodler, Leigh A., Valdez, Yanet, Rosenberger, Carrie M., Deng, Wanyin, ...
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) are noninvasive bacterial pathogens that infect their hosts' intestinal epithelium, causing severe diarrheal disease....
Crossing the Line: Selection and Evolution of Virulence Traits
Brown, Nat F, Wickham, Mark E, Coombes, Brian K, Finlay, B. Brett
The evolution of pathogens presents a paradox. Pathogenic species are often absolutely dependent on their host species for their propagation through evolutionary time, yet the pathogenic lifestyle...
Guttman, Julian A., Samji, Fereshte N., Li, Yuling, Vogl, A. Wayne, Finlay, B. Brett
It is widely accepted that tight junctions are altered during infections by attaching and effacing (A/E) pathogens. These disruptions have been demonstrated both in vitro and more recently in vivo....
Coombes, Brian K., Lowden, Michael J., Bishop, Jennifer L., Wickham, Mark E., Brown, Nat F., Duong, Nancy, ...
Bacterial pathogens use horizontal gene transfer to acquire virulence factors that influence host colonization, alter virulence traits, and ultimately shape the outcome of disease following...
Salmonella: from Pathogenesis to Therapeutics▿
Boyle, Erin C., Bishop, Jennifer L., Grassl, Guntram A., Finlay, B. Brett
Mason, David, Mallo, Gustavo V., Terebiznik, Mauricio R., Payrastre, Bernard, Finlay, B. Brett, Brumell, John H., ...
Elucidation of the role of PtdIns(4,5)P2 in epithelial function has been hampered by the inability to selectively manipulate the cellular content of this phosphoinositide. Here we report that SigD, a...
Gal-Mor, Ohad, Gibson, Deanna L., Baluta, Dan, Vallance, Bruce A., Finlay, B. Brett
Salmonella spp. are Gram-negative enteropathogenic bacteria that infect a variety of vertebrate hosts. Like any other living organism, protein secretion is a fundamental process essential for various...
Richter-Dahlfors, Agneta, Buchan, Alison M.J., Finlay, B. Brett
Salmonella typhimurium is considered a facultative intracellular pathogen, but its intracellular location in vivo has not been demonstrated conclusively. Here we describe the development of a new...
Coombes, Brian K., Wickham, Mark E., Mascarenhas, Mariola, Gruenheid, Samantha, Finlay, B. Brett, Karmali, Mohamed A.
Shiga toxin-producing Escherichia coli (STEC) strains are commensal bacteria in cattle with high potential for environmental and zoonotic transmission to humans. Although O157:H7 is the most common...
Salmonella enterica Serovar Senftenberg Human Clinical Isolates Lacking SPI-1▿
Hu, Qinghua, Coburn, Bryan, Deng, Wanyin, Li, Yuling, Shi, Xiaolu, Lan, Quanxue, ...
Nontyphoidal Salmonella species cause gastrointestinal disease worldwide. The prevailing theory of Salmonella enteropathogenesis is that bacterial invasion of the intestinal epithelium is essential...
García-Angulo, Víctor A., Deng, Wanyin, Thomas, Nikhil A., Finlay, B. Brett, Puente, Jose L.
Together with enterohemorrhagic Escherichia coli and enteropathogenic Escherichia coli, Citrobacter rodentium is a member of the attaching-and-effacing (A/E) family of bacterial pathogens. A/E...
Type III Secretion Systems and Disease
Coburn , Bryan, Sekirov, Inna, Finlay, B. Brett
Type III secretion systems (T3SSs) are complex bacterial structures that provide gram-negative pathogens with a unique virulence mechanism enabling them to inject bacterial effector proteins directly...
The Inositol Phosphatase SHIP Controls Salmonella enterica Serovar Typhimurium Infection In Vivo▿
Bishop, Jennifer L., Sly, Laura M., Krystal, Gerald, Finlay, B. Brett
The SH2 domain-containing inositol 5′-phosphatase, SHIP, negatively regulates various hematopoietic cell functions and is critical for maintaining immune homeostasis. However, whether SHIP plays a...
Mallo, Gustavo V., Espina, Marianela, Smith, Adam C., Terebiznik, Mauricio R., Alemán, Ainel, Finlay, B. Brett, ...
Salmonella colonizes a vacuolar niche in host cells during infection. Maturation of the Salmonella-containing vacuole (SCV) involves the formation of phosphatidylinositol 3-phosphate (PI(3)P) on its...
Sekirov, Inna, Tam, Nicola M., Jogova, Maria, Robertson, Marilyn L., Li, Yuling, Lupp, Claudia, ...
Intestinal microbiota comprises microbial communities that reside in the gastrointestinal tract and are critical to normal host physiology. Understanding the microbiota's role in host response to...