A histidine protein kinase is involved in polar organelle development in Caulobacter crescentus.
Wang, S P, Sharma, P L, Schoenlein, P V, Ely, B
Mutations having pleiotropic effects on polar organelle development (pod) in Caulobacter crescentus have been identified and shown to occur in at least 13 genes scattered throughout the genome....
Synthesis of the Caulobacter ferredoxin protein, FdxA, is cell cycle controlled.
Wang, S P, Kang, P J, Chen, Y P, Ely, B
The fdxA gene was identified upstream of and in the opposite direction from the Caulobacter crescentus cysC gene. Analyses of the nucleotide sequence and the deduced amino acid sequence of the fdxA...
A gene, fdxP, was identified upstream of the rrnA gene in Caulobacter crescentus and shown to encode ferredoxin II (FdII) by insertional inactivation. FdII is homologous to a class of [2Fe-2S]...
Malakooti, J, Wang, S P, Ely, B
Caulobacter crescentus differentiates prior to each cell division to form two different daughter cells: a monoflagellated swarmer cell and a nonmotile stalked cell. Thus, one might expect that...
Principal sigma subunit of the Caulobacter crescentus RNA polymerase.
We have identified the gene encoding the Caulobacter crescentus principal sigma subunit, RpoD. The rpoD gene codes for a polypeptide of 653 amino acids with a predicted molecular mass of 72,623 Da...
Malakooti, J, Ely, B, Matsumura, P
The fliL operon of Escherichia coli contains seven genes that are involved in the biosynthesis and functioning of the flagellar organelle. DNA sequences for the first three genes of this operon have...
Identification of genes affecting production of the adhesive holdfast of a marine caulobacter.
Caulobacters are stalked bacteria that produce a structure termed a holdfast which enables firm attachment to surfaces. Tn5 insertion mutagenesis was used to identify genes affecting holdfast...
The ilvR gene was located upstream of and transcribed divergently from the ilvD gene of Caulobacter crescentus. DNA nucleotide analysis determined that the ilvR and ilvD translation initiation codons...
Regulation of Caulobacter crescentus ilvBN gene expression.
Tarleton, J C, Malakooti, J, Ely, B
As part of an effort to determine the mechanisms employed by Caulobacter crescentus to regulate gene expression, the ilvBN genes encoding the two subunits of an acetohydroxy acid synthase (AHAS) have...
The differentiating bacterium Caulobacter crescentus has been studied extensively to understand how a relatively simple life form can govern the timing of expression of genes needed for the...
Isolation and characterization of ilvA, ilvBN, and ilvD mutants of Caulobacter crescentus.
Caulobacter crescentus strains requiring isoleucine and valine (ilv) for growth were shown by transduction and pulsed-field gel electrophoresis to contain mutations at one of two unlinked loci, ilvB...
Schoenlein, P V, Lui, J, Gallman, L, Ely, B
At a specific time in the Caulobacter crescentus cell cycle, a single flagellar filament and multiple receptor sites for the swarmer-specific phage phi Cbk are assembled at one pole of the...
Ohta, N, Mullin, D A, Tarleton, J, Ely, B, Newton, A
We describe two insertion elements isolated from Caulobacter crescentus that are designated IS298 and IS511. These insertion elements were cloned from spontaneous flagellar (fla) gene mutants SC298...
Circularity of the Caulobacter crescentus chromosome determined by pulsed-field gel electrophoresis.
Ely, B, Ely, T W, Gerardot, C J, Dingwall, A
Previous genetic analyses of the Caulobacter crescentus chromosome have resulted in the construction of a linear genetic map. To establish the circularity of the C. crescentus chromosome, restriction...
A Caulobacter gene involved in polar morphogenesis.
Driks, A, Schoenlein, P V, DeRosier, D J, Shapiro, L, Ely, B
At specific times in the cell cycle, the bacterium Caulobacter crescentus assembles two major polar organelles, the flagellum and the stalk. Previous studies have shown that flbT mutants overproduce...
Although widely found in the cytoplasm of eucaryotes, the copper-zinc form of superoxide dismutase (CuZnSOD) has been identified in only a small number of bacterial species. One species is the...
Schoenlein, P V, Gallman, L S, Ely, B
In Caulobacter crescentus, mutations have been isolated in more than 30 flagellar genes (fla, flb, and flg) which are required in the cell cycle event of flagellum biogenesis. The flaF and flaG...
During the Caulobacter crescentus cell cycle, flagellin synthesis and filament assembly are temporally controlled events which require the products encoded by the contiguous flaF, flbT, and flbA-flaG...
Genetic and physical analyses of Caulobacter crescentus trp genes.
Winkler, M E, Schoenlein, P V, Ross, C M, Barrett, J T, Ely, B
Caulobacter crescentus trp mutants were identified from a collection of auxotrophs. Precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification...
Ohta, N, Swanson, E, Ely, B, Newton, A
Using the cloned DNA from the hook protein gene region of Caulobacter crescentus ( Ohta et al., Proc. Natl. Acad. Sci. U.S.A. 79:4863-4867, 1982), we have identified and physically mapped 19...
Construction of a genetic map for Caulobacter crescentus.
Barrett, J T, Rhodes, C S, Ferber, D M, Jenkins, B, Kuhl, S A, Ely, B
RP4-mediated conjugation has been used to transfer large fragments of chromosomal material in Caulobacter crescentus. In this system, conjugation proceeds from multiple origins, and haploid...
Transposon mutagenesis in Caulobacter crescentus.
Transposons Tn5 (Km) and Tn7 (Tp and Sm) were transferred to Caulobacter crescentus via P-type antibiotic resistance factors. Transposition was demonstrated by the isolation of chromosomal insertions...
Synthesis and assembly of flagellar components by Caulobacter crescentus motility mutants.
Johnson, R C, Ferber, D M, Ely, B
Cultures of wild-type Caulobacter crescentus and strains with fla mutations representing 24 genes were pulse-labeled with 14C-amino acids and analyzed by immunoprecipitation to study the synthesis of...
Analysis of nonmotile mutants of the dimorphic bacterium Caulobacter crescentus.
A total of 69 spontaneous nonmotile mutants were isolated from the dimorphic bacterium Caulobacter crescentus. The majority of the mutants were unable to assemble a flagellar filament (Fla-),...
Genetic mapping with Tn5-derived auxotrophs of Caulobacter crescentus.
Barrett, J T, Croft, R H, Ferber, D M, Gerardot, C J, Schoenlein, P V, Ely, B
Chromosomal insertions of Tn5 in Caulobacter crescentus displayed complete stability upon transduction and proved useful in strain building on complex media. RP4-primes constructed in vitro...
Ammonia assimilation and glutamate formation in Caulobacter crescentus.
Ely, B, Amarasinghe, A B, Bender, R A
In the dimorphic bacterium Caulobacter crescentus, ammonia assimilation occurs only via the combined action of the enzymes glutamine synthetase and glutamate synthase. Mutants auxotrophic for...
Methylation involved in chemotaxis is regulated during Caulobacter differentiation.
Shaw, P, Gomes, S L, Sweeney, K, Ely, B, Shapiro, L
Caulobacter crescentus carries a flagellum and is motile only during a limited time in its cell cycle. We have asked if the biochemical machinery that mediates chemotaxis exists coincident with the...
Aromatic amino acid biosynthesis: regulation of shikimate kinase in Escherichia coli K-12.
Starvation of cells of Escherichia coli K-12 for the aromatic amino acids results in an increased rate of synthesis of shikimate kinase activity. The two controlling amino acids are tyrosine and...
Flagellar hook and basal complex of Caulobacter crescentus.
Johnson, R C, Walsh, M P, Ely, B, Shapiro, L
Intact bacterial flagella possessing a membrane-free hook and basal complex were purified from Caulobacter crescentus CB15, as well as from mutants which synthesize incomplete flagella. The basal...
To facilitate the mapping of transposon insertion mutations in Caulobacter crescentus, we have used pulsed field gel electrophoresis to construct a detailed physical and genetic map of the C....
A histidine protein kinase is involved in polar organelle development in Caulobacter crescentus.
Wang, S P, Sharma, P L, Schoenlein, P V, Ely, B
Mutations having pleiotropic effects on polar organelle development (pod) in Caulobacter crescentus have been identified and shown to occur in at least 13 genes scattered throughout the genome....
Synthesis of the Caulobacter ferredoxin protein, FdxA, is cell cycle controlled.
Wang, S P, Kang, P J, Chen, Y P, Ely, B
The fdxA gene was identified upstream of and in the opposite direction from the Caulobacter crescentus cysC gene. Analyses of the nucleotide sequence and the deduced amino acid sequence of the fdxA...
A gene, fdxP, was identified upstream of the rrnA gene in Caulobacter crescentus and shown to encode ferredoxin II (FdII) by insertional inactivation. FdII is homologous to a class of [2Fe-2S]...
Malakooti, J, Wang, S P, Ely, B
Caulobacter crescentus differentiates prior to each cell division to form two different daughter cells: a monoflagellated swarmer cell and a nonmotile stalked cell. Thus, one might expect that...
Principal sigma subunit of the Caulobacter crescentus RNA polymerase.
We have identified the gene encoding the Caulobacter crescentus principal sigma subunit, RpoD. The rpoD gene codes for a polypeptide of 653 amino acids with a predicted molecular mass of 72,623 Da...
Malakooti, J, Ely, B, Matsumura, P
The fliL operon of Escherichia coli contains seven genes that are involved in the biosynthesis and functioning of the flagellar organelle. DNA sequences for the first three genes of this operon have...
Identification of genes affecting production of the adhesive holdfast of a marine caulobacter.
Caulobacters are stalked bacteria that produce a structure termed a holdfast which enables firm attachment to surfaces. Tn5 insertion mutagenesis was used to identify genes affecting holdfast...
The ilvR gene was located upstream of and transcribed divergently from the ilvD gene of Caulobacter crescentus. DNA nucleotide analysis determined that the ilvR and ilvD translation initiation codons...
Regulation of Caulobacter crescentus ilvBN gene expression.
Tarleton, J C, Malakooti, J, Ely, B
As part of an effort to determine the mechanisms employed by Caulobacter crescentus to regulate gene expression, the ilvBN genes encoding the two subunits of an acetohydroxy acid synthase (AHAS) have...
The differentiating bacterium Caulobacter crescentus has been studied extensively to understand how a relatively simple life form can govern the timing of expression of genes needed for the...
Isolation and characterization of ilvA, ilvBN, and ilvD mutants of Caulobacter crescentus.
Caulobacter crescentus strains requiring isoleucine and valine (ilv) for growth were shown by transduction and pulsed-field gel electrophoresis to contain mutations at one of two unlinked loci, ilvB...
Schoenlein, P V, Lui, J, Gallman, L, Ely, B
At a specific time in the Caulobacter crescentus cell cycle, a single flagellar filament and multiple receptor sites for the swarmer-specific phage phi Cbk are assembled at one pole of the...
Ohta, N, Mullin, D A, Tarleton, J, Ely, B, Newton, A
We describe two insertion elements isolated from Caulobacter crescentus that are designated IS298 and IS511. These insertion elements were cloned from spontaneous flagellar (fla) gene mutants SC298...
Circularity of the Caulobacter crescentus chromosome determined by pulsed-field gel electrophoresis.
Ely, B, Ely, T W, Gerardot, C J, Dingwall, A
Previous genetic analyses of the Caulobacter crescentus chromosome have resulted in the construction of a linear genetic map. To establish the circularity of the C. crescentus chromosome, restriction...
A Caulobacter gene involved in polar morphogenesis.
Driks, A, Schoenlein, P V, DeRosier, D J, Shapiro, L, Ely, B
At specific times in the cell cycle, the bacterium Caulobacter crescentus assembles two major polar organelles, the flagellum and the stalk. Previous studies have shown that flbT mutants overproduce...
Although widely found in the cytoplasm of eucaryotes, the copper-zinc form of superoxide dismutase (CuZnSOD) has been identified in only a small number of bacterial species. One species is the...
Schoenlein, P V, Gallman, L S, Ely, B
In Caulobacter crescentus, mutations have been isolated in more than 30 flagellar genes (fla, flb, and flg) which are required in the cell cycle event of flagellum biogenesis. The flaF and flaG...
During the Caulobacter crescentus cell cycle, flagellin synthesis and filament assembly are temporally controlled events which require the products encoded by the contiguous flaF, flbT, and flbA-flaG...
Genetic and physical analyses of Caulobacter crescentus trp genes.
Winkler, M E, Schoenlein, P V, Ross, C M, Barrett, J T, Ely, B
Caulobacter crescentus trp mutants were identified from a collection of auxotrophs. Precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification...
Ohta, N, Swanson, E, Ely, B, Newton, A
Using the cloned DNA from the hook protein gene region of Caulobacter crescentus ( Ohta et al., Proc. Natl. Acad. Sci. U.S.A. 79:4863-4867, 1982), we have identified and physically mapped 19...
Construction of a genetic map for Caulobacter crescentus.
Barrett, J T, Rhodes, C S, Ferber, D M, Jenkins, B, Kuhl, S A, Ely, B
RP4-mediated conjugation has been used to transfer large fragments of chromosomal material in Caulobacter crescentus. In this system, conjugation proceeds from multiple origins, and haploid...
Transposon mutagenesis in Caulobacter crescentus.
Transposons Tn5 (Km) and Tn7 (Tp and Sm) were transferred to Caulobacter crescentus via P-type antibiotic resistance factors. Transposition was demonstrated by the isolation of chromosomal insertions...
Synthesis and assembly of flagellar components by Caulobacter crescentus motility mutants.
Johnson, R C, Ferber, D M, Ely, B
Cultures of wild-type Caulobacter crescentus and strains with fla mutations representing 24 genes were pulse-labeled with 14C-amino acids and analyzed by immunoprecipitation to study the synthesis of...
Aromatic amino acid biosynthesis: regulation of shikimate kinase in Escherichia coli K-12.
Starvation of cells of Escherichia coli K-12 for the aromatic amino acids results in an increased rate of synthesis of shikimate kinase activity. The two controlling amino acids are tyrosine and...
Flagellar hook and basal complex of Caulobacter crescentus.
Johnson, R C, Walsh, M P, Ely, B, Shapiro, L
Intact bacterial flagella possessing a membrane-free hook and basal complex were purified from Caulobacter crescentus CB15, as well as from mutants which synthesize incomplete flagella. The basal...
Analysis of nonmotile mutants of the dimorphic bacterium Caulobacter crescentus.
A total of 69 spontaneous nonmotile mutants were isolated from the dimorphic bacterium Caulobacter crescentus. The majority of the mutants were unable to assemble a flagellar filament (Fla-),...
Genetic mapping with Tn5-derived auxotrophs of Caulobacter crescentus.
Barrett, J T, Croft, R H, Ferber, D M, Gerardot, C J, Schoenlein, P V, Ely, B
Chromosomal insertions of Tn5 in Caulobacter crescentus displayed complete stability upon transduction and proved useful in strain building on complex media. RP4-primes constructed in vitro...
Ammonia assimilation and glutamate formation in Caulobacter crescentus.
Ely, B, Amarasinghe, A B, Bender, R A
In the dimorphic bacterium Caulobacter crescentus, ammonia assimilation occurs only via the combined action of the enzymes glutamine synthetase and glutamate synthase. Mutants auxotrophic for...
Methylation involved in chemotaxis is regulated during Caulobacter differentiation.
Shaw, P, Gomes, S L, Sweeney, K, Ely, B, Shapiro, L
Caulobacter crescentus carries a flagellum and is motile only during a limited time in its cell cycle. We have asked if the biochemical machinery that mediates chemotaxis exists coincident with the...
To facilitate the mapping of transposon insertion mutations in Caulobacter crescentus, we have used pulsed field gel electrophoresis to construct a detailed physical and genetic map of the C....