Influence of rhDNAse on the duration of mechanical ventilation in intensive care patients (2008)
Deschner, N, Friedrich, B, Brehm, W, Vonthein, R, Riethmueller, J
No abstract available.
Generation and orientation of organoxenon molecule H-Xe-CCH in the gas phase (2008)
Poterya, V., Votava, O., Fárník, M., Ončak, M., Slavíček, P., Buck, U., ...
Quo vadis, cold molecules? - Editorial review (2005)
Doyle, J., Friedrich, B., Krems, R. V., Masnou-Seeuws, F.
We give a snapshot of the rapidly developing field of ultracold polar molecules abd walk the reader through the papers appearing in this topical issue.
Burgdorf, T., Linden, E. Van, Bernhard, M., Yin, Q.Y., Back, J.W., Hartog, A.F., ...
The soluble [NiFe]-hydrogenase (SH) of the facultative lithoautotrophic proteobacterium Ralstonia eutropha H16 has up to now been described as a heterotetrameric enzyme. The purified protein consists...
Linden, E. Van Der, Faber, B.W., Bleijlevens, B., Burgdorf, T., Bernhard, M., Friedrich, B., ...
The soluble, cytoplasmic NAD+-reducing [NiFe]-hydrogenase from Ralstonia eutropha is a heterotetrameric enzyme (HoxFUYH) and contains two FMN groups. The purified oxidized enzyme is inactive in the...
Bleijlevens, B., Buhrke, T., Linden, E. Van Der, Friedrich, B., Albracht, S.P.J.
The hypX gene of the facultative lithoautotrophic bacterium Ralstonia eutropha is part of a cassette of accessory genes (the hyp cluster) required for the proper assembly of the active site of the...
Burgdorf, T., Bernhard, M., Bleijlevens, B., Friedrich, B., Albracht, S.P.J.
Infrared spectra of 15N-enriched preparations of the soluble cytoplasmic NAD+-reducing [NiFe]-hydrogenase from Ralstonia eutropha are presented. These spectra, together with chemical analyses, show...
Photodissociation of oriented HXeI molecules generated from HI-Xen clusters (2003)
Nahler,N. H., Baumfalk,R., Buck,U., Bihary,Z., Gerber,R. B., Friedrich,B.
Photodissociation of oriented HXeI molecules generated from HI-Xen clusters. (2003)
Nahler, N. H., Baumfalk, R., Buck, U., Bihary, Z., Gerber, R. B., Friedrich, B.
We report the production in the gas phase of ionically bound HXeI molecules. The molecules are generated by the photodissociation of HI molecules in large Xen clusters and are identified from the...
Tykocinski, L, Hajkova, P, Stamm, T, Sözeri, O, Löhning, M, Friedrich, B, ...
No abstract available.
Cardiopulmonary bypass and pulmonary surfactant: influence on composition and function? (2003)
Thul, J, Friedrich, B, Günther, R, Reiss, I, Schranz, D, Gortner, L
No abstract available.
Photodissociation of oriented HXeI molecules generated from HI-Xen clusters (2003)
Nahler, N. H., Baumfalk, R., Buck, U., Bihary, Z., Gerber, R. B., Friedrich, B.
Buhrke, T., Brecht, M., Lubitz, W., Friedrich, B.
[NiFe] hydrogenases contain a highly conserved histidine residue close to the [NiFe] active site which is altered by a glutamine residue in the H-2-sensing [NiFe] hydrogenases. In this study, we...
SPECTROSCOPY OF CaH IN A MAGNETIC TRAP (1999)
Weinstein, J. D., DeCarvalho, R., Doyle, J. M., Friedrich, B.
Author Institution: Department of Physics, Harvard University; Department of Chemistry and Chemical Biology, Department of Physics
Friedrich, B., Amar, K., Boca, A., DeCarvalho, R., Weinstein, J. D., Doyle, J. M.
$^{a}$ J. Kim et al., Phys. Rev. Lett. 78, 3665 (1997).
Characterization of the active site of a hydrogen sensor from Alcaligenes eutrophus (1998)
Pierik, A.J., Schmelz, M., Lenz, O., Friedrich, B., Albracht, S.P.J.
Deranged transcriptional regulation of cell-volume-sensitive kinase hSGK in diabetic nephropathy
Lang, F., Klingel, K., Wagner, C. A., Stegen, C., Wärntges, S., Friedrich, B., ...
Transforming growth factor β (TGF-β) has been shown to participate in the pathophysiology of diabetic complications. As shown most recently, TGF-β stimulates the expression of a distinct...
Structural gene (nirS) for the cytochrome cd1 nitrite reductase of Alcaligenes eutrophus H16.
Rees, E, Siddiqui, R A, Köster, F, Schneider, B, Friedrich, B
Denitrification by Alcaligenes eutrophus H16 is genetically linked to megaplasmid pHG1. Unexpectedly, the gene encoding the nitrite reductase (nirS) was identified on chromosomal DNA. The nirS...
The Alcaligenes eutrophus protein HoxN mediates nickel transport in Escherichia coli.
Wolfram, L, Friedrich, B, Eitinger, T
HoxN, an integral membrane protein with seven transmembrane helices and a molecular mass of 33.1 kDa, is involved in high-affinity nickel transport in Alcaligenes eutrophus H16. From genetic...
Zimmer, D, Schwartz, E, Tran-Betcke, A, Gewinner, P, Friedrich, B
Expression of the soluble (SH) and membrane-bound (MBH) hydrogenases in the facultatively lithoautotrophic bacterium Alcaligenes eutrophus is dependent on the transcriptional activator HoxA and the...
Thiemermann, S, Dernedde, J, Bernhard, M, Schroeder, W, Massanz, C, Friedrich, B
Two open reading frames (ORFs) were identified immediately downstream of the four structural genes for the soluble hydrogenase (SH) of Alcaligenes eutrophus H16. While a mutation in ORF2 had no...
Bernhard, M, Schwartz, E, Rietdorf, J, Friedrich, B
Alcaligenes eutrophus H16 produces two [NiFe] hydrogenases which catalyze the oxidation of hydrogen and enable the organism to utilize H2 as the sole energy source. The genes (hoxK and hoxG) for the...
Lenz, O, Strack, A, Tran-Betcke, A, Friedrich, B
Heterologous complementation studies using Alcaligenes eutrophus H16 as a recipient identified a hydrogenase-specific regulatory DNA region on megaplasmid pHG21-a of the related species Alcaligenes...
Two isofunctional nitric oxide reductases in Alcaligenes eutrophus H16.
Cramm, R, Siddiqui, R A, Friedrich, B
Two genes, norB and norZ, encoding two independent nitric oxide reductases have been identified in Alcaligenes eutrophus H16. norB and norZ predict polypeptides of 84.5 kDa with amino acid sequence...
The Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation.
Lenz, O, Schwartz, E, Dernedde, J, Eitinger, M, Friedrich, B
Nucleotide sequence analysis revealed a 1,791-bp open reading frame in the hox gene cluster of the gram-negative chemolithotroph Alcaligenes eutrophus H16. In order to investigate the biological role...
Expression of bacterial mercuric ion reductase in Saccharomyces cerevisiae.
Rensing, C, Kües, U, Stahl, U, Nies, D H, Friedrich, B
The gene merA coding for bacterial mercuric ion reductase was cloned under the control of the yeast promoter for alcohol dehydrogenase I in the yeast-Escherichia coli shuttle plasmid pADH040-2 and...
Structure and function of a periplasmic nitrate reductase in Alcaligenes eutrophus H16.
Siddiqui, R A, Warnecke-Eberz, U, Hengsberger, A, Schneider, B, Kostka, S, Friedrich, B
Alcaligenes eutrophus H16 shows three distinct nitrate reductase activities (U. Warnecke-Eberz and B. Friedrich, Arch. Microbiol. 159:405-409, 1993). The periplasmic enzyme, designated NAP (nitrate...
A gene complex coding for the membrane-bound hydrogenase of Alcaligenes eutrophus H16.
Kortlüke, C, Horstmann, K, Schwartz, E, Rohde, M, Binsack, R, Friedrich, B
One of the key enzymes in the chemolithoautotrophic metabolism of Alcaligenes eutrophus H16 is a dimeric, membrane-associated hydrogenase. The genetic determinants of this enzyme are located on the...
Maturation of membrane-bound hydrogenase of Alcaligenes eutrophus H16.
The formation of the catalytically active membrane-bound hydrogenase (MBH) of Alcaligenes eutrophus H16 requires the genes for the small and large subunits of the enzyme (hoxK and hoxG, respectively)...
Three trans-acting regulatory functions control hydrogenase synthesis in Alcaligenes eutrophus.
Random Tn5 mutagenesis of the regulatory region of megaplasmid pHG1 of Alcaligenes eutrophus led to the identification of three distinct loci designated hoxA, hoxD, and hoxE. Sequencing of the hoxA...
Tran-Betcke, A, Warnecke, U, Böcker, C, Zaborosch, C, Friedrich, B
The genes hoxF, -U, -Y, and -H which encode the four subunit polypeptides alpha, gamma, delta, and beta of the NAD-reducing hydrogenase (HoxS) of Alcaligenes eutrophus H16, were cloned, expressed in...
Römermann, D, Warrelmann, J, Bender, R A, Friedrich, B
Pleiotropic mutants of Alcaligenes eutrophus with the phenotype Hno- have been characterized previously. They are deficient in several diverse metabolic activities, including hydrogen oxidation,...
Eberz, G, Eitinger, T, Friedrich, B
Nickel-deficient (Nic-) mutants of Alcaligenes eutrophus requiring high levels of nickel ions for autotrophic growth with hydrogen were characterized. The Nic- mutants carried defined deletions in...
Regulation of assimilatory nitrate reductase formation in Klebsiella aerogenes W70.
Klebsiella aerogenes W70 could grow aerobically with nitrate or nitrite as the sole nitrogen source. The assimilatory nitrate reductase and nitrite reductase responsible for this ability required the...
Molecular cloning of structural and regulatory hydrogenase (hox) genes of Alcaligenes eutrophus H16.
Eberz, G, Hogrefe, C, Kortlüke, C, Kamienski, A, Friedrich, B
A gene bank of the 450-kilobase (kb) megaplasmid pHG1 from the hydrogen-oxidizing bacterium Alcaligenes eutrophus H16 was constructed in the broad-host-range mobilizable vector pSUP202 and maintained...
Nies, D, Mergeay, M, Friedrich, B, Schlegel, H G
A 238-kilobase-pair plasmid, pMOL30, confers resistance to cadmium, zinc, and cobalt salts in Alcaligenes eutrophus CH34. After Tn5 mutagenesis, restriction nuclease analysis, and Southern DNA-DNA...
Alcaligenes eutrophus hydrogenase genes (Hox).
Hogrefe, C, Römermann, D, Friedrich, B
Mutants of Alcaligenes eutrophus H16 lacking catalytically active soluble hydrogenase (Hos-) grew very slowly lithoautotrophically with hydrogen. Mutants devoid of particulate hydrogenase activity...
Expression of hydrogenase in Alcaligenes spp. is altered by interspecific plasmid exchange.
Friedrich, B, Friedrich, C G, Meyer, M, Schlegel, H G
Alcaligenes hydrogenophilus was found to contain a soluble and a particulate hydrogenase whose control and structure differed in part from that in Alcaligenes eutrophus. One of at least two plasmids...
Friedrich, B, Hogrefe, C, Schlegel, H G
Mutants defective in chemolithoautotrophic growth (Aut-) have been isolated from Alcaligenes eutrophus strains H16, N9A, G27, and TF93. Spontaneous Aut- mutants were obtained only with strain TF93....
Nickel requirement for active hydrogenase formation in Alcaligenes eutrophus.
Friedrich, B, Heine, E, Finck, A, Friedrich, C G
The nickel-dependent chemolithoautotrophic growth of Alcaligenes eutrophus is apparently due to a requirement of nickel for active hydrogenase formation. Cells grown heterotrophically with fructose...
Alcaligenes eutrophus grew well autotrophically with molecular hydrogen at 30 degrees C, but failed to grow at 37 degrees C (Hox Ts). At this temperature the strain grew well heterotrophically with a...
Enzymes of agmatine degradation and the control of their synthesis in Klebsiella aerogenes.
The degradation of agmatine to succinate by Klebsiella aerogenes occurs in five steps. The enzyme catalyzing the first step, agmatinase, is induced by agmatine. The enzymes catalyzing the second and...
Denitrification by Alcaligenes eutrophus is plasmid dependent.
Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability...
Nickel in the catalytically active hydrogenase of Alcaligenes eutrophus.
Friedrich, C G, Schneider, K, Friedrich, B
Nickel is a constituent of soluble and particulate hydrogenase of Alcaligenes eutrophus. Incorporation of 63Ni2+ revealed that almost the total nickel taken up by the cells was bound to the protein....
Utilization of arginine by Klebsiella aerogenes.
Klebsiella aerogenes utilized arginine as the sole source of carbon or nitrogen for growth. Arginine was degraded to 2-ketoglutarate and not to succinate, since a citrate synthaseless mutant grows on...
Friedrich, B, Friedrich, C G, Magasanik, B
Klebsiella aerogenes formed two N2-acetylornithine 5-aminotransferases (ACOAT) which were separable by diethylaminoethyl-cellulose chromatography. One ACOAT was repressed when the cells grew on...
Friedrich, B, Friedrich, C G, Schlegel, H G
Chorismate mutase and prephenate dehydratase from Alcaligenes autophus H16 were purified 470-fold with a yield of 24%. During the course of purification, including chromatography on diethylaminoethyl...
Friedrich, C G, Friedrich, B, Schlegel, H G
Highly purified enzymes from Alcaligenes eutrophus H 16 were used for kinetic studies. Chorismate mutase was feedback inhibited by phenylalanine. In the absence of the inhibitor, the...
Urease of Klebsiella aerogenes: control of its synthesis by glutamine synthetase.
Urease was purified 24-fold from extracts of Klebsiella aerogenes. The enzyme has a molecular weight of 230,000 as determined by gel filtration, is highly substrate specific, and has a Km for urea of...
Humoral and cellular defense against intestinal murine infection with Yersinia enterocolitica.
Hanski, C, Naumann, M, Grützkau, A, Pluschke, G, Friedrich, B, Hahn, H, ...
The role of phagocytes and the complement system as potential host defense mechanisms against bacterial infection were studied in mice with two isogenic strains of Yersinia enterocolitica serotype O8...
Crystal structure of the flavohemoglobin from Alcaligenes eutrophus at 1.75 A resolution.
Ermler, U, Siddiqui, R A, Cramm, R, Friedrich, B
The molecular structure of the flavohemoglobin from Alcaligenes eutrophus has been determined to a resolution of 1.75 A and refined to an R-factor of 19.6%. The protein comprises two fused modules: a...
Dimmer, K S, Friedrich, B, Lang, F, Deitmer, J W, Bröer, S
Transport of lactate and other monocarboxylates in mammalian cells is mediated by a family of transporters, designated monocarboxylate transporters (MCTs). The MCT4 member of this family has recently...
Association of 4F2hc with light chains LAT1, LAT2 or y+LAT2 requires different domains.
Bröer, A, Friedrich, B, Wagner, C A, Fillon, S, Ganapathy, V, Lang, F, ...
Heterodimeric amino acid transporters are comprised of a type-II membrane protein named the heavy chain (4F2hc or rBAT) that may associate with a number of different polytopic membrane proteins,...
Deranged transcriptional regulation of cell-volume-sensitive kinase hSGK in diabetic nephropathy
Lang, F., Klingel, K., Wagner, C. A., Stegen, C., Wärntges, S., Friedrich, B., ...
Transforming growth factor β (TGF-β) has been shown to participate in the pathophysiology of diabetic complications. As shown most recently, TGF-β stimulates the expression of a distinct...
Structural gene (nirS) for the cytochrome cd1 nitrite reductase of Alcaligenes eutrophus H16.
Rees, E, Siddiqui, R A, Köster, F, Schneider, B, Friedrich, B
Denitrification by Alcaligenes eutrophus H16 is genetically linked to megaplasmid pHG1. Unexpectedly, the gene encoding the nitrite reductase (nirS) was identified on chromosomal DNA. The nirS...
The Alcaligenes eutrophus protein HoxN mediates nickel transport in Escherichia coli.
Wolfram, L, Friedrich, B, Eitinger, T
HoxN, an integral membrane protein with seven transmembrane helices and a molecular mass of 33.1 kDa, is involved in high-affinity nickel transport in Alcaligenes eutrophus H16. From genetic...
Zimmer, D, Schwartz, E, Tran-Betcke, A, Gewinner, P, Friedrich, B
Expression of the soluble (SH) and membrane-bound (MBH) hydrogenases in the facultatively lithoautotrophic bacterium Alcaligenes eutrophus is dependent on the transcriptional activator HoxA and the...
Thiemermann, S, Dernedde, J, Bernhard, M, Schroeder, W, Massanz, C, Friedrich, B
Two open reading frames (ORFs) were identified immediately downstream of the four structural genes for the soluble hydrogenase (SH) of Alcaligenes eutrophus H16. While a mutation in ORF2 had no...
Bernhard, M, Schwartz, E, Rietdorf, J, Friedrich, B
Alcaligenes eutrophus H16 produces two [NiFe] hydrogenases which catalyze the oxidation of hydrogen and enable the organism to utilize H2 as the sole energy source. The genes (hoxK and hoxG) for the...
Lenz, O, Strack, A, Tran-Betcke, A, Friedrich, B
Heterologous complementation studies using Alcaligenes eutrophus H16 as a recipient identified a hydrogenase-specific regulatory DNA region on megaplasmid pHG21-a of the related species Alcaligenes...
Two isofunctional nitric oxide reductases in Alcaligenes eutrophus H16.
Cramm, R, Siddiqui, R A, Friedrich, B
Two genes, norB and norZ, encoding two independent nitric oxide reductases have been identified in Alcaligenes eutrophus H16. norB and norZ predict polypeptides of 84.5 kDa with amino acid sequence...
The Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation.
Lenz, O, Schwartz, E, Dernedde, J, Eitinger, M, Friedrich, B
Nucleotide sequence analysis revealed a 1,791-bp open reading frame in the hox gene cluster of the gram-negative chemolithotroph Alcaligenes eutrophus H16. In order to investigate the biological role...
Expression of bacterial mercuric ion reductase in Saccharomyces cerevisiae.
Rensing, C, Kües, U, Stahl, U, Nies, D H, Friedrich, B
The gene merA coding for bacterial mercuric ion reductase was cloned under the control of the yeast promoter for alcohol dehydrogenase I in the yeast-Escherichia coli shuttle plasmid pADH040-2 and...
Structure and function of a periplasmic nitrate reductase in Alcaligenes eutrophus H16.
Siddiqui, R A, Warnecke-Eberz, U, Hengsberger, A, Schneider, B, Kostka, S, Friedrich, B
Alcaligenes eutrophus H16 shows three distinct nitrate reductase activities (U. Warnecke-Eberz and B. Friedrich, Arch. Microbiol. 159:405-409, 1993). The periplasmic enzyme, designated NAP (nitrate...
A gene complex coding for the membrane-bound hydrogenase of Alcaligenes eutrophus H16.
Kortlüke, C, Horstmann, K, Schwartz, E, Rohde, M, Binsack, R, Friedrich, B
One of the key enzymes in the chemolithoautotrophic metabolism of Alcaligenes eutrophus H16 is a dimeric, membrane-associated hydrogenase. The genetic determinants of this enzyme are located on the...
Maturation of membrane-bound hydrogenase of Alcaligenes eutrophus H16.
The formation of the catalytically active membrane-bound hydrogenase (MBH) of Alcaligenes eutrophus H16 requires the genes for the small and large subunits of the enzyme (hoxK and hoxG, respectively)...
Three trans-acting regulatory functions control hydrogenase synthesis in Alcaligenes eutrophus.
Random Tn5 mutagenesis of the regulatory region of megaplasmid pHG1 of Alcaligenes eutrophus led to the identification of three distinct loci designated hoxA, hoxD, and hoxE. Sequencing of the hoxA...
Tran-Betcke, A, Warnecke, U, Böcker, C, Zaborosch, C, Friedrich, B
The genes hoxF, -U, -Y, and -H which encode the four subunit polypeptides alpha, gamma, delta, and beta of the NAD-reducing hydrogenase (HoxS) of Alcaligenes eutrophus H16, were cloned, expressed in...
Römermann, D, Warrelmann, J, Bender, R A, Friedrich, B
Pleiotropic mutants of Alcaligenes eutrophus with the phenotype Hno- have been characterized previously. They are deficient in several diverse metabolic activities, including hydrogen oxidation,...
Eberz, G, Eitinger, T, Friedrich, B
Nickel-deficient (Nic-) mutants of Alcaligenes eutrophus requiring high levels of nickel ions for autotrophic growth with hydrogen were characterized. The Nic- mutants carried defined deletions in...
Regulation of assimilatory nitrate reductase formation in Klebsiella aerogenes W70.
Klebsiella aerogenes W70 could grow aerobically with nitrate or nitrite as the sole nitrogen source. The assimilatory nitrate reductase and nitrite reductase responsible for this ability required the...
Molecular cloning of structural and regulatory hydrogenase (hox) genes of Alcaligenes eutrophus H16.
Eberz, G, Hogrefe, C, Kortlüke, C, Kamienski, A, Friedrich, B
A gene bank of the 450-kilobase (kb) megaplasmid pHG1 from the hydrogen-oxidizing bacterium Alcaligenes eutrophus H16 was constructed in the broad-host-range mobilizable vector pSUP202 and maintained...
Nies, D, Mergeay, M, Friedrich, B, Schlegel, H G
A 238-kilobase-pair plasmid, pMOL30, confers resistance to cadmium, zinc, and cobalt salts in Alcaligenes eutrophus CH34. After Tn5 mutagenesis, restriction nuclease analysis, and Southern DNA-DNA...
Alcaligenes eutrophus hydrogenase genes (Hox).
Hogrefe, C, Römermann, D, Friedrich, B
Mutants of Alcaligenes eutrophus H16 lacking catalytically active soluble hydrogenase (Hos-) grew very slowly lithoautotrophically with hydrogen. Mutants devoid of particulate hydrogenase activity...
Expression of hydrogenase in Alcaligenes spp. is altered by interspecific plasmid exchange.
Friedrich, B, Friedrich, C G, Meyer, M, Schlegel, H G
Alcaligenes hydrogenophilus was found to contain a soluble and a particulate hydrogenase whose control and structure differed in part from that in Alcaligenes eutrophus. One of at least two plasmids...
Friedrich, B, Hogrefe, C, Schlegel, H G
Mutants defective in chemolithoautotrophic growth (Aut-) have been isolated from Alcaligenes eutrophus strains H16, N9A, G27, and TF93. Spontaneous Aut- mutants were obtained only with strain TF93....
Nickel requirement for active hydrogenase formation in Alcaligenes eutrophus.
Friedrich, B, Heine, E, Finck, A, Friedrich, C G
The nickel-dependent chemolithoautotrophic growth of Alcaligenes eutrophus is apparently due to a requirement of nickel for active hydrogenase formation. Cells grown heterotrophically with fructose...
Alcaligenes eutrophus grew well autotrophically with molecular hydrogen at 30 degrees C, but failed to grow at 37 degrees C (Hox Ts). At this temperature the strain grew well heterotrophically with a...
Enzymes of agmatine degradation and the control of their synthesis in Klebsiella aerogenes.
The degradation of agmatine to succinate by Klebsiella aerogenes occurs in five steps. The enzyme catalyzing the first step, agmatinase, is induced by agmatine. The enzymes catalyzing the second and...
Denitrification by Alcaligenes eutrophus is plasmid dependent.
Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability...
Nickel in the catalytically active hydrogenase of Alcaligenes eutrophus.
Friedrich, C G, Schneider, K, Friedrich, B
Nickel is a constituent of soluble and particulate hydrogenase of Alcaligenes eutrophus. Incorporation of 63Ni2+ revealed that almost the total nickel taken up by the cells was bound to the protein....
Utilization of arginine by Klebsiella aerogenes.
Klebsiella aerogenes utilized arginine as the sole source of carbon or nitrogen for growth. Arginine was degraded to 2-ketoglutarate and not to succinate, since a citrate synthaseless mutant grows on...
Friedrich, B, Friedrich, C G, Magasanik, B
Klebsiella aerogenes formed two N2-acetylornithine 5-aminotransferases (ACOAT) which were separable by diethylaminoethyl-cellulose chromatography. One ACOAT was repressed when the cells grew on...
Friedrich, B, Friedrich, C G, Schlegel, H G
Chorismate mutase and prephenate dehydratase from Alcaligenes autophus H16 were purified 470-fold with a yield of 24%. During the course of purification, including chromatography on diethylaminoethyl...
Friedrich, C G, Friedrich, B, Schlegel, H G
Highly purified enzymes from Alcaligenes eutrophus H 16 were used for kinetic studies. Chorismate mutase was feedback inhibited by phenylalanine. In the absence of the inhibitor, the...
Urease of Klebsiella aerogenes: control of its synthesis by glutamine synthetase.
Urease was purified 24-fold from extracts of Klebsiella aerogenes. The enzyme has a molecular weight of 230,000 as determined by gel filtration, is highly substrate specific, and has a Km for urea of...
Humoral and cellular defense against intestinal murine infection with Yersinia enterocolitica.
Hanski, C, Naumann, M, Grützkau, A, Pluschke, G, Friedrich, B, Hahn, H, ...
The role of phagocytes and the complement system as potential host defense mechanisms against bacterial infection were studied in mice with two isogenic strains of Yersinia enterocolitica serotype O8...
Crystal structure of the flavohemoglobin from Alcaligenes eutrophus at 1.75 A resolution.
Ermler, U, Siddiqui, R A, Cramm, R, Friedrich, B
The molecular structure of the flavohemoglobin from Alcaligenes eutrophus has been determined to a resolution of 1.75 A and refined to an R-factor of 19.6%. The protein comprises two fused modules: a...
Dimmer, K S, Friedrich, B, Lang, F, Deitmer, J W, Bröer, S
Transport of lactate and other monocarboxylates in mammalian cells is mediated by a family of transporters, designated monocarboxylate transporters (MCTs). The MCT4 member of this family has recently...
Association of 4F2hc with light chains LAT1, LAT2 or y+LAT2 requires different domains.
Bröer, A, Friedrich, B, Wagner, C A, Fillon, S, Ganapathy, V, Lang, F, ...
Heterodimeric amino acid transporters are comprised of a type-II membrane protein named the heavy chain (4F2hc or rBAT) that may associate with a number of different polytopic membrane proteins,...
Chronic sinusitis refractory to standard management in patients with humoral immunodeficiencies
BUEHRING, I, FRIEDRICH, B, SCHAAF, J, SCHMIDT, H, AHRENS, P, ZIELEN, S
Chronic refractory sinusitis is a common feature in patients with primary immunodeficiencies. The efficacy of standard therapeutic strategies is questionable. In an open trial we evaluated the...