The M-Integral for Computing Stress in Generally Anisotropic Materials (2005)
Wawrzynek, P.A., Carter, B.J., Banks-Sills, L.
Single-crystal super alloys are commonly used for components in the hot sections of contemporary jet and rocket engines. Due to the anisotropic nature of single-crystal materials the use of existing...
Star Formation in a Complete Spectroscopic Survey of Galaxies (2001)
Carter, B. J., Fabricant, D. G., Geller, M. J., Kurtz, M. J.
The 15R-North galaxy redshift survey is a uniform spectroscopic survey (S/N $\sim $10) covering the range 3650---7400\AA for 3149 galaxies with median redshift 0.05. The sample is 90% complete to...
Automated 3-d crack growth simulation (2000)
B. J. Carter, P. A. Wawrzynek, A. R. Ingraffea
Automated simulation of arbitrary, non-planar, 3D crack growth in real-life engineered structures requires two key components: crack representation and crack growth mechanics. A model environment for...
Power Subsystem Trade Studies for a 5 kW MEO Application. (1998)
Thaller, L. H., Carter, B. J., Halle, L. F., Zimmerman, A. H.
A technology trade study for a large generic MEO spacecraft has been carried out. The objective of the study was to address the main factors related to a modern, but not cutting edge, spacecraft...
Flotte, T R, Afione, S A, Conrad, C, McGrath, S A, Solow, R, Oka, H, ...
Adeno-associated virus (AAV) vectors expressing the normal cystic fibrosis transmembrane conductance regulator (CFTR) cDNA complement the cystic fibrosis (CF) defect in vitro. Unlike other DNA virus...
Site-specific cleavage of RNA by Fe(II).bleomycin.
Carter, B J, De Vroom, E, Long, E C, Van Der Marel, G A, Van Boom, J H, Hecht, S M
Bleomycin is an antitumor agent whose activity has long been thought to derive from its ability to degrade DNA. Recent findings suggest that cellular RNA may be a therapeutically relevant locus. At...
Interaction of wild-type and mutant adeno-associated virus (AAV) Rep proteins on AAV hairpin DNA.
Weitzman, M D, Kyöstiö, S R, Carter, B J, Owens, R A
Both the Rep68 and Rep78 proteins of adeno-associated virus type 2 (AAV) bind to AAV terminal repeat hairpin DNA and can mediate site-specific nicking in vitro at the terminal resolution site (trs)...
In vivo model of adeno-associated virus vector persistence and rescue.
Afione, S A, Conrad, C K, Kearns, W G, Chunduru, S, Adams, R, Reynolds, T C, ...
Gene therapy vectors based on human DNA viruses could be mobilized or rescued from individuals who are subsequently infected with the corresponding wild-type (wt) helper viruses. This phenomenon has...
Kyöstiö, S R, Owens, R A, Weitzman, M D, Antoni, B A, Chejanovsky, N, Carter, B J
The rep gene of adeno-associated virus type 2 (AAV) encodes four overlapping Rep proteins that are involved in gene regulation and replication of the virus. We studied here the regulation of mRNA...
Identification of a DNA-binding domain in the amino terminus of adeno-associated virus Rep proteins.
Owens, R A, Weitzman, M D, Kyöstiö, S R, Carter, B J
The Rep78 and Rep68 proteins of adeno-associated virus (AAV) bind to the AAV terminal repeat hairpin DNA and are required for viral replication. We have expressed a series of mutant rep genes from...
Antoni, B A, Rabson, A B, Miller, I L, Trempe, J P, Chejanovsky, N, Carter, B J
The adeno-associated virus (AAV) rep gene encodes four proteins (Rep78, Rep68, Rep52, and Rep40) required for AAV DNA replication and AAV gene regulation. In addition, the Rep proteins may have...
An adeno-associated virus (AAV) genome with a Lys-to-His (K340H) mutation in the consensus nucleotide triphosphate binding site of the rep gene has a dominant-negative DNA replication phenotype in...
Adeno-associated virus rep protein synthesis during productive infection.
Redemann, B E, Mendelson, E, Carter, B J
Adeno-associated virus (AAV) Rep proteins mediate viral DNA replication and can regulate expression from AAV genes. We studied the kinetics of synthesis of the four Rep proteins, Rep78, Rep68, Rep52,...
Adeno-associated virus (AAV) contains a multifunctional nonstructural gene, rep, which is required for AAV DNA replication and has pleiotropic effects on positive and negative regulation of gene...
We studied the effects of the adeno-associated virus (AAV) rep gene on the control of gene expression from the AAV p40 promoter in 293 cells in the absence of an adenovirus coinfection. AAV vectors...
Mendelson, E, Trempe, J P, Carter, B J
Prior genetic analysis provided evidence for trans-acting regulatory proteins (Rep) coded by the left-hand open reading frame (orf-1) of adeno-associated virus (AAV). We have used immunoblotting...
Alternate mRNA splicing is required for synthesis of adeno-associated virus VP1 capsid protein.
Fine-structure mapping of the capsid-specific mRNAs from adeno-associated virus (AAV) revealed an alternate splicing pattern in these RNAs. S1 nuclease and primer extension analyses showed that...
Chromatin-like structure of adeno-associated virus DNA in infected cells.
Marcus-Sekura, C J, Carter, B J
Adeno-associated virus (AAV) is a single-stranded DNA virus which requires adenovirus as a helper for productive infection. We studied whether intracellular AAV DNA in KB cells was present in a...
Tratschin, J D, Miller, I L, Carter, B J
Transfection of a pBR322-based, recombinant plasmid, pAV2, containing the entire adeno-associated virus (AAV) type 2 genome into human 293 cells in the presence of helper adenovirus resulted in...
Effect of deletions in adenovirus early region 1 genes upon replication of adeno-associated virus.
Laughlin, C A, Jones, N, Carter, B J
The growth of adeno-associated virus (AAV) is dependent upon helper functions provided by adenovirus. We investigated the role of adenovirus early gene region 1 in the AAV helper function by using...
Myers, M W, Laughlin, C A, Jay, F T, Carter, B J
Adeno-associated virus (AAV) grows efficiently only in cells that are also infected with an adenovirus (Ad). We employed Ad mutants to determine which genes may be required for the AAV helper...
Jay, F T, Laughlin, C A, Carter, B J
Growth of adeno-associated virus (AAV) requires expression of certain adenovirus (Ad) genes in the same cell. AAV particles contain three proteins, VP1 (Mr 85,700), VP2 (Mr 72,000), and VP3 (Mr...
Watson, R J, Colberg-Poley, A M, Marcus-Sekura, C J, Carter, B J, Enquist, L W
We have identified and characterized a 3.0 kilobase (kb) mRNA containing coding sequences of the herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) gene. The synthesis of this 3.0 kb mRNA was...
Cleavage of adeno-associated virus DNA with Sali,Psti and Haeii restriction endonucleases.
Duplex AAV-2 DNA was digested with SalI, PstI or HaeII restriction endonucleases and the cleavage sites were mapped. SalI cleaves AAV DNA at 0.310 map units, PstI at 0.106, 0.422 and 0.914 and the...
Richardson, W D, Carter, B J, Westphal, H
Adenovirus type 2 mRNAs were injected via glass capillaries into Vero cells, a line of African green monkey kidney cells permissive for adenovirus growth. Polypeptides synthesized after injection...
Carter, B J, Khoury, G, Denhardt, D T
Cleavage of adenovirus-associated virus type 2 (AAV2) DNA linear duplex monomers with the restriction endonuclease R-EcoRI yielded three fragments, A, B, and C, having approximate mol wt of 1.6 X...
Genome localization of adeno-associated virus RNA.
Carter, B J, Fife, K H, De La Maza, L M, Berns, K I
In previous work, linear duplex molecules of adeno-associated virus, type 2 (AAV2), DNA were cleaved with the restriction endonucleases R-EcoRI, R-HindII, and R-HindIII. The physical order of the...
Tratschin, J D, Tal, J, Carter, B J
We previously described use of the human parvovirus, adeno-associated virus (AAV), as a vector for transient expression in mammalian cells of the gene for chloramphenicol acetyltransferase (CAT). In...
Tratschin, J D, West, M H, Sandbank, T, Carter, B J
We have used the defective human parvovirus adeno-associated virus (AAV) as a novel eucaryotic vector (parvector) for the expression of a foreign gene in human cells. The recombinant, pAV2, contains...
Tratschin, J D, Miller, I L, Smith, M G, Carter, B J
We describe the construction of an adeno-associated virus (AAV) vector in which the coding sequence of the procaryotic gene neo is expressed under the control of the major AAV promoter p40. This...
Jay, F T, De La Maza, L M, Carter, B J
We report here a method of RNA preparation that may enrich for precursor RNA sequences and the results of an investigation of adeno-associated virus (AAV) RNA transcription that used this method....
Spliced adenovirus-associated virus RNA.
Laughlin, C A, Westphal, H, Carter, B J
We describe the structure of cytoplasmic RNA species transcribed from the DNA of adenovirus-associated virus, a defective parvovirus. The RNA was hybridized with minus strand template DNA and...
Genome localization of simian virus 40 RNA species.
Khoury, G, Carter, B J, Ferdinand, F J, Howley, P M, Brown, M, Martin, M A
The topographical locations on the simian virus 40 (SV40) genome of the templates for virus-specific RNA species present late in the lytic infection were determined by RNA-DNA hybridization...
Denhardt, D T, Eisenberg, S, Bartok, K, Carter, B J
The double-stranded form of adeno-associated virus (AAV) DNA has about 20 sites sensitive to endonuclease R.Hae III from Haemophilus aegypitus; the fragments produced fall into about 13 size classes,...
Flotte, T R, Afione, S A, Conrad, C, McGrath, S A, Solow, R, Oka, H, ...
Adeno-associated virus (AAV) vectors expressing the normal cystic fibrosis transmembrane conductance regulator (CFTR) cDNA complement the cystic fibrosis (CF) defect in vitro. Unlike other DNA virus...
Site-specific cleavage of RNA by Fe(II).bleomycin.
Carter, B J, De Vroom, E, Long, E C, Van Der Marel, G A, Van Boom, J H, Hecht, S M
Bleomycin is an antitumor agent whose activity has long been thought to derive from its ability to degrade DNA. Recent findings suggest that cellular RNA may be a therapeutically relevant locus. At...
Interaction of wild-type and mutant adeno-associated virus (AAV) Rep proteins on AAV hairpin DNA.
Weitzman, M D, Kyöstiö, S R, Carter, B J, Owens, R A
Both the Rep68 and Rep78 proteins of adeno-associated virus type 2 (AAV) bind to AAV terminal repeat hairpin DNA and can mediate site-specific nicking in vitro at the terminal resolution site (trs)...
In vivo model of adeno-associated virus vector persistence and rescue.
Afione, S A, Conrad, C K, Kearns, W G, Chunduru, S, Adams, R, Reynolds, T C, ...
Gene therapy vectors based on human DNA viruses could be mobilized or rescued from individuals who are subsequently infected with the corresponding wild-type (wt) helper viruses. This phenomenon has...
Kyöstiö, S R, Owens, R A, Weitzman, M D, Antoni, B A, Chejanovsky, N, Carter, B J
The rep gene of adeno-associated virus type 2 (AAV) encodes four overlapping Rep proteins that are involved in gene regulation and replication of the virus. We studied here the regulation of mRNA...
Identification of a DNA-binding domain in the amino terminus of adeno-associated virus Rep proteins.
Owens, R A, Weitzman, M D, Kyöstiö, S R, Carter, B J
The Rep78 and Rep68 proteins of adeno-associated virus (AAV) bind to the AAV terminal repeat hairpin DNA and are required for viral replication. We have expressed a series of mutant rep genes from...
Antoni, B A, Rabson, A B, Miller, I L, Trempe, J P, Chejanovsky, N, Carter, B J
The adeno-associated virus (AAV) rep gene encodes four proteins (Rep78, Rep68, Rep52, and Rep40) required for AAV DNA replication and AAV gene regulation. In addition, the Rep proteins may have...
An adeno-associated virus (AAV) genome with a Lys-to-His (K340H) mutation in the consensus nucleotide triphosphate binding site of the rep gene has a dominant-negative DNA replication phenotype in...
Adeno-associated virus rep protein synthesis during productive infection.
Redemann, B E, Mendelson, E, Carter, B J
Adeno-associated virus (AAV) Rep proteins mediate viral DNA replication and can regulate expression from AAV genes. We studied the kinetics of synthesis of the four Rep proteins, Rep78, Rep68, Rep52,...
Adeno-associated virus (AAV) contains a multifunctional nonstructural gene, rep, which is required for AAV DNA replication and has pleiotropic effects on positive and negative regulation of gene...
We studied the effects of the adeno-associated virus (AAV) rep gene on the control of gene expression from the AAV p40 promoter in 293 cells in the absence of an adenovirus coinfection. AAV vectors...
Mendelson, E, Trempe, J P, Carter, B J
Prior genetic analysis provided evidence for trans-acting regulatory proteins (Rep) coded by the left-hand open reading frame (orf-1) of adeno-associated virus (AAV). We have used immunoblotting...
Alternate mRNA splicing is required for synthesis of adeno-associated virus VP1 capsid protein.
Fine-structure mapping of the capsid-specific mRNAs from adeno-associated virus (AAV) revealed an alternate splicing pattern in these RNAs. S1 nuclease and primer extension analyses showed that...
Chromatin-like structure of adeno-associated virus DNA in infected cells.
Marcus-Sekura, C J, Carter, B J
Adeno-associated virus (AAV) is a single-stranded DNA virus which requires adenovirus as a helper for productive infection. We studied whether intracellular AAV DNA in KB cells was present in a...
Tratschin, J D, Miller, I L, Carter, B J
Transfection of a pBR322-based, recombinant plasmid, pAV2, containing the entire adeno-associated virus (AAV) type 2 genome into human 293 cells in the presence of helper adenovirus resulted in...
Effect of deletions in adenovirus early region 1 genes upon replication of adeno-associated virus.
Laughlin, C A, Jones, N, Carter, B J
The growth of adeno-associated virus (AAV) is dependent upon helper functions provided by adenovirus. We investigated the role of adenovirus early gene region 1 in the AAV helper function by using...
Myers, M W, Laughlin, C A, Jay, F T, Carter, B J
Adeno-associated virus (AAV) grows efficiently only in cells that are also infected with an adenovirus (Ad). We employed Ad mutants to determine which genes may be required for the AAV helper...
Jay, F T, Laughlin, C A, Carter, B J
Growth of adeno-associated virus (AAV) requires expression of certain adenovirus (Ad) genes in the same cell. AAV particles contain three proteins, VP1 (Mr 85,700), VP2 (Mr 72,000), and VP3 (Mr...
Watson, R J, Colberg-Poley, A M, Marcus-Sekura, C J, Carter, B J, Enquist, L W
We have identified and characterized a 3.0 kilobase (kb) mRNA containing coding sequences of the herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) gene. The synthesis of this 3.0 kb mRNA was...
Cleavage of adeno-associated virus DNA with Sali,Psti and Haeii restriction endonucleases.
Duplex AAV-2 DNA was digested with SalI, PstI or HaeII restriction endonucleases and the cleavage sites were mapped. SalI cleaves AAV DNA at 0.310 map units, PstI at 0.106, 0.422 and 0.914 and the...
Richardson, W D, Carter, B J, Westphal, H
Adenovirus type 2 mRNAs were injected via glass capillaries into Vero cells, a line of African green monkey kidney cells permissive for adenovirus growth. Polypeptides synthesized after injection...
Carter, B J, Khoury, G, Denhardt, D T
Cleavage of adenovirus-associated virus type 2 (AAV2) DNA linear duplex monomers with the restriction endonuclease R-EcoRI yielded three fragments, A, B, and C, having approximate mol wt of 1.6 X...
Genome localization of adeno-associated virus RNA.
Carter, B J, Fife, K H, De La Maza, L M, Berns, K I
In previous work, linear duplex molecules of adeno-associated virus, type 2 (AAV2), DNA were cleaved with the restriction endonucleases R-EcoRI, R-HindII, and R-HindIII. The physical order of the...
Tratschin, J D, Tal, J, Carter, B J
We previously described use of the human parvovirus, adeno-associated virus (AAV), as a vector for transient expression in mammalian cells of the gene for chloramphenicol acetyltransferase (CAT). In...
Tratschin, J D, West, M H, Sandbank, T, Carter, B J
We have used the defective human parvovirus adeno-associated virus (AAV) as a novel eucaryotic vector (parvector) for the expression of a foreign gene in human cells. The recombinant, pAV2, contains...
Tratschin, J D, Miller, I L, Smith, M G, Carter, B J
We describe the construction of an adeno-associated virus (AAV) vector in which the coding sequence of the procaryotic gene neo is expressed under the control of the major AAV promoter p40. This...
Jay, F T, De La Maza, L M, Carter, B J
We report here a method of RNA preparation that may enrich for precursor RNA sequences and the results of an investigation of adeno-associated virus (AAV) RNA transcription that used this method....
Spliced adenovirus-associated virus RNA.
Laughlin, C A, Westphal, H, Carter, B J
We describe the structure of cytoplasmic RNA species transcribed from the DNA of adenovirus-associated virus, a defective parvovirus. The RNA was hybridized with minus strand template DNA and...
Genome localization of simian virus 40 RNA species.
Khoury, G, Carter, B J, Ferdinand, F J, Howley, P M, Brown, M, Martin, M A
The topographical locations on the simian virus 40 (SV40) genome of the templates for virus-specific RNA species present late in the lytic infection were determined by RNA-DNA hybridization...
Denhardt, D T, Eisenberg, S, Bartok, K, Carter, B J
The double-stranded form of adeno-associated virus (AAV) DNA has about 20 sites sensitive to endonuclease R.Hae III from Haemophilus aegypitus; the fragments produced fall into about 13 size classes,...