Potentiation of the bioavailability of daidzin by an extract of Radix puerariae.
Keung, W M, Lazo, O, Kunze, L, Vallee, B L
The dose effect of pure daidzin on the suppression of ethanol intake in Syrian golden hamsters was compared with that of crude daidzin contained in a methanol extract of Radix puerariae (RP). EC50...
Nobile, V, Vallee, B L, Shapiro, R
Angiogenin-related protein (Angrp), the putative product of a recently discovered mouse gene, shares 78% sequence identity with mouse angiogenin (Ang). In the present study, the relationship of Angrp...
Auld, D S, Falchuk, K H, Zhang, K, Montorzi, M, Vallee, B L
The x-ray absorption fine structure (XAFS) zinc K-edge steps for intact stages I,II and V,VI Xenopus laevis oocytes demonstrate that the zinc concentration is about 3 and 1 mM, respectively. However,...
The C-terminal region of human angiogenin has a dual role in enzymatic activity.
Russo, N, Nobile, V, Di Donato, A, Riordan, J F, Vallee, B L
The ribonucleolytic activity of angiogenin (Ang) is essential to Ang's capacity to induce blood vessel formation. Previous x-ray diffraction and mutagenesis results have shown that the active site of...
A combined kinetic and modeling study of the catalytic center subsites of human angiogenin.
Russo, N, Acharya, K R, Vallee, B L, Shapiro, R
Kinetic analysis and molecular modeling have been used to map the ribonucleolytic center of angiogenin (Ang). Pyrimidine nucleotides were found to interact very weakly with Ang, consistent with the...
Keung, W M, Lazo, O, Kunze, L, Vallee, B L
Daidzin is a potent, selective, and reversible inhibitor of human mitochondrial aldehyde dehydrogenase (ALDH) that suppresses free-choice ethanol intake by Syrian golden hamsters. Other ALDH...
Crystal structure of bovine angiogenin at 1.5-A resolution.
Acharya, K R, Shapiro, R, Riordan, J F, Vallee, B L
The capacity of angiogenin (Ang) to induce blood vessel growth is critically dependent on its ribonucleolytic activity. Crystallography and mutagenesis of human Ang have previously shown that its...
Angiogenin antagonists prevent tumor growth in vivo.
Olson, K A, Fett, J W, French, T C, Key, M E, Vallee, B L
A noncytotoxic neutralizing monoclonal antibody (mAb), 26-2F, to human angiogenin (Ang), a potent inducer of neovascularization, has been reported to prevent or delay the establishment of HT-29 human...
Cell cycle regulation of metallothionein in human colonic cancer cells.
Elevated levels of metallothionein (MT) found in rapidly growing tissues such as neonatal liver and various types of human tumors have suggested a role for MT in cell proliferation. To further...
Acharya, K R, Shapiro, R, Allen, S C, Riordan, J F, Vallee, B L
Angiogenin, a potent inducer of neovascularization, is the only angiogenic molecule known to exhibit ribonucleolytic activity. Its overall structure, as determined at 2.4 A, is similar to that of...
Role of glutamine-117 in the ribonucleolytic activity of human angiogenin.
Russo, N, Shapiro, R, Acharya, K R, Riordan, J F, Vallee, B L
The crystal structure of human angiogenin (reported in the preceding paper in this issue) reveals that the site that corresponds to the pyrimidine binding site of RNase A is obstructed by Gln-117....
Hu, G, Riordan, J F, Vallee, B L
Angiogenin, a potent inducer of neovascularization in the chicken chorioallantoic membrane and rabbit cornea, promotes endothelial cell invasion of Matrigel basement membrane. A transformed bovine...
Actin is a binding protein for angiogenin.
Hu, G F, Strydom, D J, Fett, J W, Riordan, J F, Vallee, B L
The 42-kDa angiogenin binding protein isolated previously has been purified to electrophoretic homogeneity. It has been identified as a member of the actin family by peptide mapping and partial amino...
Daidzin: a potent, selective inhibitor of human mitochondrial aldehyde dehydrogenase.
Human mitochondrial aldehyde dehydrogenase (ALDH-I) is potently, reversibly, and selectively inhibited by an isoflavone isolated from Radix puerariae and identified as daidzin, the 7-glucoside of...
Engeland, K, Höög, J O, Holmquist, B, Estonius, M, Jörnvall, H, Vallee, B L
The origin of the fatty acid activation and formaldehyde dehydrogenase activity that distinguishes human class III alcohol dehydrogenase (alcohol:NAD+ oxidoreductase, EC 1.1.1.1) from all other...
Cocatalytic zinc motifs in enzyme catalysis.
Cocatalytic zinc binding sites are characteristic of enzyme molecules which contain two or more zinc and/or other metal atoms. In each site an aspartate, glutamate, or histidine residue...
Actin is a surface component of calf pulmonary artery endothelial cells in culture.
Moroianu, J, Fett, J W, Riordan, J F, Vallee, B L
An angiogenin binding protein isolated previously from endothelial cells has been shown to be a member of the actin family. Calf pulmonary artery endothelial (CPAE) cells were investigated for the...
Daidzin and daidzein suppress free-choice ethanol intake by Syrian golden hamsters.
Syrian Golden hamsters prefer and consume large and remarkably constant amounts of ethanol in a simple two-bottle free-choice regimen. Ethanol intake is significantly suppressed by zimelidine,...
Leukotriene A4 hydrolase: abrogation of the peptidase activity by mutation of glutamic acid-296.
Wetterholm, A, Medina, J F, Rådmark, O, Shapiro, R, Haeggström, J Z, Vallee, B L, ...
The metal-binding motif in the sequence of leukotriene A4 (LTA4) (EC 3.3.2.6), a bifunctional zinc metalloenzyme, contains a glutamic acid that is conserved in several zinc hydrolases. To study its...
Zinc rapidly induces a metal response element-binding factor.
Czupryn, M, Brown, W E, Vallee, B L
Metal activation of metallothionein gene transcription is mediated by specific promoter sequences, termed metal regulatory elements (MREs). Nuclear extracts prepared from various human cell lines...
Zinc fingers, zinc clusters, and zinc twists in DNA-binding protein domains.
Vallee, B L, Coleman, J E, Auld, D S
We now recognize three distinct motifs of DNA-binding zinc proteins: (i) zinc fingers, (ii) zinc clusters, and (iii) zinc twists. Until very recently, x-ray crystallographic or NMR three-dimensional...
Dual site model for the organogenic activity of angiogenin.
Hallahan, T W, Shapiro, R, Vallee, B L
The residues that are indispensable for the ribonucleolytic activity of angiogenin are also known to be essential for its angiogenic activity. We now demonstrate that residues in another region of...
An angiogenin-binding protein from endothelial cells.
Hu, G F, Chang, S I, Riordan, J F, Vallee, B L
A 42-kDa bovine protein that binds bovine angiogenin [angiogenin binding protein (AngBP)] has been identified as a dissociable cell-surface component of calf pulmonary artery endothelial cells and a...
Medina, J F, Wetterholm, A, Rådmark, O, Shapiro, R, Haeggström, J Z, Vallee, B L, ...
Three mutants of recombinant mouse leukotriene A4 (LTA4) hydrolase (3.3.2.6) were produced by site-directed mutagenesis on cDNA. The codons corresponding to His-295, His-299, or Glu-318 were replaced...
Zinc transfer from transcription factor IIIA fingers to thionein clusters.
Zeng, J, Vallee, B L, Kägi, J H
The rapid induction of thionein (apometallothionein) by many endogenous stimuli such as steroid hormones, cytokines, and second messengers suggests that this cysteine-rich, metal binding protein...
Active-site zinc ligands and activated H2O of zinc enzymes.
The x-ray crystallographic structures of 12 zinc enzymes have been chosen as standards of reference to identify the ligands to the catalytic and structural zinc atoms of other members of their...
Modular mutagenesis of human placental ribonuclease inhibitor, a protein with leucine-rich repeats.
Human placental ribonuclease inhibitor (PRI) is a potent protein inhibitor of pancreatic ribonucleases and the homologous blood vessel-inducing protein angiogenin. Although inhibition by PRI occurs...
Structural and functional zinc in horse liver alcohol dehydrogenase.
Drum, D E, Harrison, J H, Li, T K, Bethune, J L, Vallee, B L
Angiogenin activates endothelial cell phospholipase C.
Low concentrations of angiogenin activate the inositol-specific phospholipase C of cultured pulmonary artery, umbilical vein, and capillary endothelial cells, promoting a transient increase in the...
Site-specific mutagenesis of the blood vessel-inducing protein angiogenin has been used to further explore both its homology to pancreatic ribonuclease and the functional roles of particular...
Surveyor Substrates: Energy-Transfer Gauges of Active Center Topography during Catalysis*
Latt, S. A., Auld, D. S., Vallee, B. L.
An approach is described for the simultaneous measurement of catalysis, the distance from an active-site moiety to fluorescent substrate moieties of an enzyme substrate complex, and the...
Angiogenin stimulates endothelial cell prostacyclin secretion by activation of phospholipase A2.
Angiogenin stimulates capillary and umbilical vein endothelial cell prostacyclin secretion but not that of prostaglandins of the E series. The response was quantitated by radioimmunoassay and by...
Heath, W F, Moore, F, Bicknell, R, Vallee, B L
125I-labeled angiogenin binds rapidly to the plasma membrane of several cell lines at 37 degrees C (t1/2 less than 1 min) but in comparatively small amounts. Competition with unlabeled angiogenin...
St Clair, D K, Rybak, S M, Riordan, J F, Vallee, B L
Angiogenin is a potent inhibitor of cell-free protein synthesis. When incubated with rabbit reticulocyte lysate at a concentration of 40-60 nM, it completely abolishes the capacity of the lysate to...
Ribonucleolytic activity of angiogenin: essential histidine, lysine, and arginine residues.
Shapiro, R, Weremowicz, S, Riordan, J F, Vallee, B L
The homology of angiogenin and pancreatic RNase A provides a compelling reason to systematically compare the characteristics of the two proteins using the chemical modification approaches that proved...
Human placental ribonuclease inhibitor (PRI) abolishes both the ribonucleolytic activity of angiogenin toward 28S and 18S rRNA and its angiogenic activity on the chicken embryo chorioallantoic...
Jörnvall, H, Höög, J O, Vallee, B L
A comparison of the structure of class II human liver alcohol dehydrogenase (alcohol:NAD+ oxidoreductase, EC 1.1.1.1) (containing pi subunits) with those of the human class I isozymes (containing...
A preliminary three-dimensional structure of angiogenin.
Palmer, K A, Scheraga, H A, Riordan, J F, Vallee, B L
A preliminary three-dimensional structure of angiogenin has been computed, based on its homology to bovine pancreatic ribonuclease A. A standard-geometry structure of ribonuclease was first obtained...
Mårdh, G, Falchuk, K H, Auld, D S, Vallee, B L
Testosterone and its physiologically active metabolite 5 alpha-dihydrotestosterone are selective, allosteric inhibitors of the gamma subunit-containing isozymes of class I human alcohol dehydrogenase...
Horse liver alcohol dehydrogenase (EC 1.1.1.1) contains one catalytic and one noncatalytic pair of zinc atoms that can be replaced selectively with cobalt and/or 65zince. We have now prepared...
Falchuk, K H, Hardy, C, Ulpino, L, Vallee, B L
The three major RNA classes from zinc-sufficient [(+Zn)] and zinc-deficient [(=Zn)] Euglena gracilis have been separated by affinity chromatography on oligo(dT)- and...
Jörnvall, H, Hempel, J, Vallee, B L, Bosron, W F, Li, T K
The homodimeric Oriental beta 2 beta 2 isozyme of human liver alcohol dehydrogenase, corresponding to an allelic variant at the ADH2 gene locus, was studied in order to define the amino acid exchange...
Structure and function of carboxypeptidase A alpha in supercooled water.
Thompson, J S, Gehring, H, Vallee, B L
The spectral and enzymatic characteristics of chromophoric derivatives of carboxypeptidase A alpha (EC 3.4.17.1) have been examined at subzero temperatures in supercooled water-in-oil emulsions....
Digitalis metabolism and human liver alcohol dehydrogenase.
Human liver alcohol dehydrogenase (alcohol: NAD" oxidoreductase, EC 1.1.1.1) catalyzes the oxidation of the 3 beta-OH group of digitoxigenin, digoxigenin, and gitoxigenin to their 3-keto derivatives,...
Bosron, W F, Li, T K, Vallee, B L
The biochemical determinants of alcoholism and genetic correlates for the variability in man's response to alcohol have remained obscure until recently. The identification of genetically determined...
Mårdh, G, Dingley, A L, Auld, D S, Vallee, B L
Studies of the function of human alcohol dehydrogenase (ADH) have revealed substrates that are virtually unique for class II ADH (pi ADH). It catalyzes the formation of the intermediary glycols of...
Auld, D. S., Kawaguchi, H., Livingston, D. M., Vallee, B. L.
RNA tumor viruses contain a characteristic RNA-dependent DNA polymerase (reverse transcriptase) which has been thought to be related to the induction of leukemia by this virus. A disturbance in a...
Harrison, L W, Auld, D S, Vallee, B L
The red azoTyr-248-Zn complex of arnilazocarboxypeptidase, previously used to demonstrate differences in conformation of the enzyme in crystals and in solution, has now provided means to detect...
Horrocks, W D, Holmquist, B, Vallee, B L
The visible fluorescence of terbium(III) when bound to a calcium binding site of thermolysin is greatly enhanced with an excitation maximum at 280 nm but substitution of cobalt(II) for zinc at the...
Mårdh, G, Luehr, C A, Vallee, B L
Investigations of the function of human liver alcohol dehydrogenase (ADH) in norepinephrine metabolism have revealed that class I ADH catalyzes the oxidation of the intermediary alcohols...
chi-ADH is the sole alcohol dehydrogenase isozyme of mammalian brains: implications and inferences.
Beisswenger, T B, Holmquist, B, Vallee, B L
Class III (chi) is the only alcohol dehydrogenase (ADH) in human, equine, bovine, simian, canine, and rodent brain and is the first to be identified, purified, and characterized from the brain of...
Cryospectrokinetic characterization of intermediates in biochemical reactions: carboxypeptidase A.
Auld, D S, Galdes, A, Geoghegan, K F, Holmquist, B, Martinelli, R A, Vallee, B L
Cryospectrokinetic studies provide concurrent structural, kinetic, and chemical data on short-lived intermediates in the course of the interactions of enzymes with their substrates and of other,...
Bühler, R, Hempel, J, Kaiser, R, Von Wartburg, J P, Vallee, B L, Jörnvall, H
Human alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase, EC 1.1.1.1) occurs in multiple forms, which exhibit distinct electrophoretic mobilities and enzymatic properties. The homogeneous...
Metal-coordinating substrate analogs as inhibitors of metalloenzymes.
A group of active-site metal coordinating inhibitors of zinc proteases (carboxypeptidase A, thermolysin, Bacillus cereus neutral protease, and angiotensin-converting enzyme) have been synthesized and...
Amino-acid sequence of equine renal metallothionein-1B.
Kojima, Y, Berger, C, Vallee, B L, Kägi, J H
The amino-acid sequence of a metallothionein is reported. Metallothionein is a widely distributed, extremely cysteine-rich, low-molecular-weight protein containing large amounts of cadmium and/or...
Scheule, R. K., Wart, H. E. Van, Vallee, B. L., Scheraga, H. A.
Resonance Raman spectra of arsanilazotryosyl-248 carboxypeptidase A (peptidyl-L-amino-acid hydrolase, EC 3.4.12.2) exhibit only the vibrational bands of its chromophoric azotyrosyl-248 residue...
Cobalt(III), a probe of metal binding sites of Escherichia coli alkaline phosphatase.
To facilitate the study of individual metal binding sites of polymeric metalloproteins, conversion of exchange-labile Co(II) in E. coli alkaline phosphatase (EC 3.1.3.1) to exchange-inert Co(III) was...
Role of magnesium in Escherichia coli alkaline phosphatase.
Anderson, R A, Bosron, W F, Kennedy, F S, Vallee, B L
Alkaline phosphatase of E. coli, isolated by procedures which do not alter its intrinsic metal content, contains 1.3 +/- 0.3 g-atom of magnesium and 4.0 +/- 0.2 g-atom of zinc per molecule of...
Harrison, L W, Auld, D S, Vallee, B L
The intensely chromophoric intramolecular coordination complex formed between arsanilazotyrosine-248 and the active site zinc atom of azocarboxypeptidase A (Johansen, J. T. & Vallee, B. L. (1971)...
Conformations of Arsanilazotyrosine-248 Carboxypeptidase Aα,β,γ. Comparison of Crystals and Solution
Johansen, J. T., Vallee, B. L.
The spectra of the α, β, and γ forms of zinc monoarsanilazotyrosine-248 carboxypeptidase A are indistinguishable. At pH 8.2 their crystals are yellow, while their solutions are red, λmax 510 nm....
Weremowicz, S, Fox, E A, Morton, C C, Vallee, B L
The gene encoding angiogenin, a potent inducer of blood vessel formation, has been localized within the human genome. It is present as a single copy per haploid genome and is located on chromosome...
Potentiation of the bioavailability of daidzin by an extract of Radix puerariae.
Keung, W M, Lazo, O, Kunze, L, Vallee, B L
The dose effect of pure daidzin on the suppression of ethanol intake in Syrian golden hamsters was compared with that of crude daidzin contained in a methanol extract of Radix puerariae (RP). EC50...
Nobile, V, Vallee, B L, Shapiro, R
Angiogenin-related protein (Angrp), the putative product of a recently discovered mouse gene, shares 78% sequence identity with mouse angiogenin (Ang). In the present study, the relationship of Angrp...
Auld, D S, Falchuk, K H, Zhang, K, Montorzi, M, Vallee, B L
The x-ray absorption fine structure (XAFS) zinc K-edge steps for intact stages I,II and V,VI Xenopus laevis oocytes demonstrate that the zinc concentration is about 3 and 1 mM, respectively. However,...
The C-terminal region of human angiogenin has a dual role in enzymatic activity.
Russo, N, Nobile, V, Di Donato, A, Riordan, J F, Vallee, B L
The ribonucleolytic activity of angiogenin (Ang) is essential to Ang's capacity to induce blood vessel formation. Previous x-ray diffraction and mutagenesis results have shown that the active site of...
A combined kinetic and modeling study of the catalytic center subsites of human angiogenin.
Russo, N, Acharya, K R, Vallee, B L, Shapiro, R
Kinetic analysis and molecular modeling have been used to map the ribonucleolytic center of angiogenin (Ang). Pyrimidine nucleotides were found to interact very weakly with Ang, consistent with the...
Keung, W M, Lazo, O, Kunze, L, Vallee, B L
Daidzin is a potent, selective, and reversible inhibitor of human mitochondrial aldehyde dehydrogenase (ALDH) that suppresses free-choice ethanol intake by Syrian golden hamsters. Other ALDH...
Crystal structure of bovine angiogenin at 1.5-A resolution.
Acharya, K R, Shapiro, R, Riordan, J F, Vallee, B L
The capacity of angiogenin (Ang) to induce blood vessel growth is critically dependent on its ribonucleolytic activity. Crystallography and mutagenesis of human Ang have previously shown that its...
Angiogenin antagonists prevent tumor growth in vivo.
Olson, K A, Fett, J W, French, T C, Key, M E, Vallee, B L
A noncytotoxic neutralizing monoclonal antibody (mAb), 26-2F, to human angiogenin (Ang), a potent inducer of neovascularization, has been reported to prevent or delay the establishment of HT-29 human...
Cell cycle regulation of metallothionein in human colonic cancer cells.
Elevated levels of metallothionein (MT) found in rapidly growing tissues such as neonatal liver and various types of human tumors have suggested a role for MT in cell proliferation. To further...
Acharya, K R, Shapiro, R, Allen, S C, Riordan, J F, Vallee, B L
Angiogenin, a potent inducer of neovascularization, is the only angiogenic molecule known to exhibit ribonucleolytic activity. Its overall structure, as determined at 2.4 A, is similar to that of...
Role of glutamine-117 in the ribonucleolytic activity of human angiogenin.
Russo, N, Shapiro, R, Acharya, K R, Riordan, J F, Vallee, B L
The crystal structure of human angiogenin (reported in the preceding paper in this issue) reveals that the site that corresponds to the pyrimidine binding site of RNase A is obstructed by Gln-117....
Hu, G, Riordan, J F, Vallee, B L
Angiogenin, a potent inducer of neovascularization in the chicken chorioallantoic membrane and rabbit cornea, promotes endothelial cell invasion of Matrigel basement membrane. A transformed bovine...
Actin is a binding protein for angiogenin.
Hu, G F, Strydom, D J, Fett, J W, Riordan, J F, Vallee, B L
The 42-kDa angiogenin binding protein isolated previously has been purified to electrophoretic homogeneity. It has been identified as a member of the actin family by peptide mapping and partial amino...
Daidzin: a potent, selective inhibitor of human mitochondrial aldehyde dehydrogenase.
Human mitochondrial aldehyde dehydrogenase (ALDH-I) is potently, reversibly, and selectively inhibited by an isoflavone isolated from Radix puerariae and identified as daidzin, the 7-glucoside of...
Engeland, K, Höög, J O, Holmquist, B, Estonius, M, Jörnvall, H, Vallee, B L
The origin of the fatty acid activation and formaldehyde dehydrogenase activity that distinguishes human class III alcohol dehydrogenase (alcohol:NAD+ oxidoreductase, EC 1.1.1.1) from all other...
Cocatalytic zinc motifs in enzyme catalysis.
Cocatalytic zinc binding sites are characteristic of enzyme molecules which contain two or more zinc and/or other metal atoms. In each site an aspartate, glutamate, or histidine residue...
Actin is a surface component of calf pulmonary artery endothelial cells in culture.
Moroianu, J, Fett, J W, Riordan, J F, Vallee, B L
An angiogenin binding protein isolated previously from endothelial cells has been shown to be a member of the actin family. Calf pulmonary artery endothelial (CPAE) cells were investigated for the...
Daidzin and daidzein suppress free-choice ethanol intake by Syrian golden hamsters.
Syrian Golden hamsters prefer and consume large and remarkably constant amounts of ethanol in a simple two-bottle free-choice regimen. Ethanol intake is significantly suppressed by zimelidine,...
Leukotriene A4 hydrolase: abrogation of the peptidase activity by mutation of glutamic acid-296.
Wetterholm, A, Medina, J F, Rådmark, O, Shapiro, R, Haeggström, J Z, Vallee, B L, ...
The metal-binding motif in the sequence of leukotriene A4 (LTA4) (EC 3.3.2.6), a bifunctional zinc metalloenzyme, contains a glutamic acid that is conserved in several zinc hydrolases. To study its...
Zinc rapidly induces a metal response element-binding factor.
Czupryn, M, Brown, W E, Vallee, B L
Metal activation of metallothionein gene transcription is mediated by specific promoter sequences, termed metal regulatory elements (MREs). Nuclear extracts prepared from various human cell lines...
Zinc fingers, zinc clusters, and zinc twists in DNA-binding protein domains.
Vallee, B L, Coleman, J E, Auld, D S
We now recognize three distinct motifs of DNA-binding zinc proteins: (i) zinc fingers, (ii) zinc clusters, and (iii) zinc twists. Until very recently, x-ray crystallographic or NMR three-dimensional...
Dual site model for the organogenic activity of angiogenin.
Hallahan, T W, Shapiro, R, Vallee, B L
The residues that are indispensable for the ribonucleolytic activity of angiogenin are also known to be essential for its angiogenic activity. We now demonstrate that residues in another region of...
An angiogenin-binding protein from endothelial cells.
Hu, G F, Chang, S I, Riordan, J F, Vallee, B L
A 42-kDa bovine protein that binds bovine angiogenin [angiogenin binding protein (AngBP)] has been identified as a dissociable cell-surface component of calf pulmonary artery endothelial cells and a...
Medina, J F, Wetterholm, A, Rådmark, O, Shapiro, R, Haeggström, J Z, Vallee, B L, ...
Three mutants of recombinant mouse leukotriene A4 (LTA4) hydrolase (3.3.2.6) were produced by site-directed mutagenesis on cDNA. The codons corresponding to His-295, His-299, or Glu-318 were replaced...
Zinc transfer from transcription factor IIIA fingers to thionein clusters.
Zeng, J, Vallee, B L, Kägi, J H
The rapid induction of thionein (apometallothionein) by many endogenous stimuli such as steroid hormones, cytokines, and second messengers suggests that this cysteine-rich, metal binding protein...
Active-site zinc ligands and activated H2O of zinc enzymes.
The x-ray crystallographic structures of 12 zinc enzymes have been chosen as standards of reference to identify the ligands to the catalytic and structural zinc atoms of other members of their...
Modular mutagenesis of human placental ribonuclease inhibitor, a protein with leucine-rich repeats.
Human placental ribonuclease inhibitor (PRI) is a potent protein inhibitor of pancreatic ribonucleases and the homologous blood vessel-inducing protein angiogenin. Although inhibition by PRI occurs...
Structural and functional zinc in horse liver alcohol dehydrogenase.
Drum, D E, Harrison, J H, Li, T K, Bethune, J L, Vallee, B L
Angiogenin activates endothelial cell phospholipase C.
Low concentrations of angiogenin activate the inositol-specific phospholipase C of cultured pulmonary artery, umbilical vein, and capillary endothelial cells, promoting a transient increase in the...
Site-specific mutagenesis of the blood vessel-inducing protein angiogenin has been used to further explore both its homology to pancreatic ribonuclease and the functional roles of particular...
Surveyor Substrates: Energy-Transfer Gauges of Active Center Topography during Catalysis*
Latt, S. A., Auld, D. S., Vallee, B. L.
An approach is described for the simultaneous measurement of catalysis, the distance from an active-site moiety to fluorescent substrate moieties of an enzyme substrate complex, and the...
Angiogenin stimulates endothelial cell prostacyclin secretion by activation of phospholipase A2.
Angiogenin stimulates capillary and umbilical vein endothelial cell prostacyclin secretion but not that of prostaglandins of the E series. The response was quantitated by radioimmunoassay and by...
Heath, W F, Moore, F, Bicknell, R, Vallee, B L
125I-labeled angiogenin binds rapidly to the plasma membrane of several cell lines at 37 degrees C (t1/2 less than 1 min) but in comparatively small amounts. Competition with unlabeled angiogenin...
St Clair, D K, Rybak, S M, Riordan, J F, Vallee, B L
Angiogenin is a potent inhibitor of cell-free protein synthesis. When incubated with rabbit reticulocyte lysate at a concentration of 40-60 nM, it completely abolishes the capacity of the lysate to...
Ribonucleolytic activity of angiogenin: essential histidine, lysine, and arginine residues.
Shapiro, R, Weremowicz, S, Riordan, J F, Vallee, B L
The homology of angiogenin and pancreatic RNase A provides a compelling reason to systematically compare the characteristics of the two proteins using the chemical modification approaches that proved...
Human placental ribonuclease inhibitor (PRI) abolishes both the ribonucleolytic activity of angiogenin toward 28S and 18S rRNA and its angiogenic activity on the chicken embryo chorioallantoic...
Jörnvall, H, Höög, J O, Vallee, B L
A comparison of the structure of class II human liver alcohol dehydrogenase (alcohol:NAD+ oxidoreductase, EC 1.1.1.1) (containing pi subunits) with those of the human class I isozymes (containing...
A preliminary three-dimensional structure of angiogenin.
Palmer, K A, Scheraga, H A, Riordan, J F, Vallee, B L
A preliminary three-dimensional structure of angiogenin has been computed, based on its homology to bovine pancreatic ribonuclease A. A standard-geometry structure of ribonuclease was first obtained...
Mårdh, G, Falchuk, K H, Auld, D S, Vallee, B L
Testosterone and its physiologically active metabolite 5 alpha-dihydrotestosterone are selective, allosteric inhibitors of the gamma subunit-containing isozymes of class I human alcohol dehydrogenase...
Horse liver alcohol dehydrogenase (EC 1.1.1.1) contains one catalytic and one noncatalytic pair of zinc atoms that can be replaced selectively with cobalt and/or 65zince. We have now prepared...
Falchuk, K H, Hardy, C, Ulpino, L, Vallee, B L
The three major RNA classes from zinc-sufficient [(+Zn)] and zinc-deficient [(=Zn)] Euglena gracilis have been separated by affinity chromatography on oligo(dT)- and...
Jörnvall, H, Hempel, J, Vallee, B L, Bosron, W F, Li, T K
The homodimeric Oriental beta 2 beta 2 isozyme of human liver alcohol dehydrogenase, corresponding to an allelic variant at the ADH2 gene locus, was studied in order to define the amino acid exchange...
Structure and function of carboxypeptidase A alpha in supercooled water.
Thompson, J S, Gehring, H, Vallee, B L
The spectral and enzymatic characteristics of chromophoric derivatives of carboxypeptidase A alpha (EC 3.4.17.1) have been examined at subzero temperatures in supercooled water-in-oil emulsions....
Digitalis metabolism and human liver alcohol dehydrogenase.
Human liver alcohol dehydrogenase (alcohol: NAD" oxidoreductase, EC 1.1.1.1) catalyzes the oxidation of the 3 beta-OH group of digitoxigenin, digoxigenin, and gitoxigenin to their 3-keto derivatives,...
Bosron, W F, Li, T K, Vallee, B L
The biochemical determinants of alcoholism and genetic correlates for the variability in man's response to alcohol have remained obscure until recently. The identification of genetically determined...
Mårdh, G, Dingley, A L, Auld, D S, Vallee, B L
Studies of the function of human alcohol dehydrogenase (ADH) have revealed substrates that are virtually unique for class II ADH (pi ADH). It catalyzes the formation of the intermediary glycols of...
Auld, D. S., Kawaguchi, H., Livingston, D. M., Vallee, B. L.
RNA tumor viruses contain a characteristic RNA-dependent DNA polymerase (reverse transcriptase) which has been thought to be related to the induction of leukemia by this virus. A disturbance in a...
Harrison, L W, Auld, D S, Vallee, B L
The red azoTyr-248-Zn complex of arnilazocarboxypeptidase, previously used to demonstrate differences in conformation of the enzyme in crystals and in solution, has now provided means to detect...
Horrocks, W D, Holmquist, B, Vallee, B L
The visible fluorescence of terbium(III) when bound to a calcium binding site of thermolysin is greatly enhanced with an excitation maximum at 280 nm but substitution of cobalt(II) for zinc at the...
Mårdh, G, Luehr, C A, Vallee, B L
Investigations of the function of human liver alcohol dehydrogenase (ADH) in norepinephrine metabolism have revealed that class I ADH catalyzes the oxidation of the intermediary alcohols...
chi-ADH is the sole alcohol dehydrogenase isozyme of mammalian brains: implications and inferences.
Beisswenger, T B, Holmquist, B, Vallee, B L
Class III (chi) is the only alcohol dehydrogenase (ADH) in human, equine, bovine, simian, canine, and rodent brain and is the first to be identified, purified, and characterized from the brain of...
Cryospectrokinetic characterization of intermediates in biochemical reactions: carboxypeptidase A.
Auld, D S, Galdes, A, Geoghegan, K F, Holmquist, B, Martinelli, R A, Vallee, B L
Cryospectrokinetic studies provide concurrent structural, kinetic, and chemical data on short-lived intermediates in the course of the interactions of enzymes with their substrates and of other,...
Bühler, R, Hempel, J, Kaiser, R, Von Wartburg, J P, Vallee, B L, Jörnvall, H
Human alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase, EC 1.1.1.1) occurs in multiple forms, which exhibit distinct electrophoretic mobilities and enzymatic properties. The homogeneous...
Metal-coordinating substrate analogs as inhibitors of metalloenzymes.
A group of active-site metal coordinating inhibitors of zinc proteases (carboxypeptidase A, thermolysin, Bacillus cereus neutral protease, and angiotensin-converting enzyme) have been synthesized and...
Amino-acid sequence of equine renal metallothionein-1B.
Kojima, Y, Berger, C, Vallee, B L, Kägi, J H
The amino-acid sequence of a metallothionein is reported. Metallothionein is a widely distributed, extremely cysteine-rich, low-molecular-weight protein containing large amounts of cadmium and/or...
Scheule, R. K., Wart, H. E. Van, Vallee, B. L., Scheraga, H. A.
Resonance Raman spectra of arsanilazotryosyl-248 carboxypeptidase A (peptidyl-L-amino-acid hydrolase, EC 3.4.12.2) exhibit only the vibrational bands of its chromophoric azotyrosyl-248 residue...
Cobalt(III), a probe of metal binding sites of Escherichia coli alkaline phosphatase.
To facilitate the study of individual metal binding sites of polymeric metalloproteins, conversion of exchange-labile Co(II) in E. coli alkaline phosphatase (EC 3.1.3.1) to exchange-inert Co(III) was...
Role of magnesium in Escherichia coli alkaline phosphatase.
Anderson, R A, Bosron, W F, Kennedy, F S, Vallee, B L
Alkaline phosphatase of E. coli, isolated by procedures which do not alter its intrinsic metal content, contains 1.3 +/- 0.3 g-atom of magnesium and 4.0 +/- 0.2 g-atom of zinc per molecule of...
Harrison, L W, Auld, D S, Vallee, B L
The intensely chromophoric intramolecular coordination complex formed between arsanilazotyrosine-248 and the active site zinc atom of azocarboxypeptidase A (Johansen, J. T. & Vallee, B. L. (1971)...
Conformations of Arsanilazotyrosine-248 Carboxypeptidase Aα,β,γ. Comparison of Crystals and Solution
Johansen, J. T., Vallee, B. L.
The spectra of the α, β, and γ forms of zinc monoarsanilazotyrosine-248 carboxypeptidase A are indistinguishable. At pH 8.2 their crystals are yellow, while their solutions are red, λmax 510 nm....
Weremowicz, S, Fox, E A, Morton, C C, Vallee, B L
The gene encoding angiogenin, a potent inducer of blood vessel formation, has been localized within the human genome. It is present as a single copy per haploid genome and is located on chromosome...
Human angiogenin, an organogenic protein.
Angiogenin is a 14 kD protein, initially isolated as a tumour-cell secreted product but subsequently found to be a normal constituent of human plasma. It is a potent inducer of blood vessel formation...