In vitro selection of RNAs that bind to the human immunodeficiency virus type-1 gag polyprotein.
Lochrie, M A, Waugh, S, Pratt, D G, Clever, J, Parslow, T G, Polisky, B
RNA ligands that bind to the human immunodeficiency virus type-1 (HIV-1) gag polyprotein with 10(-9) M affinity were isolated from a complex pool of RNAs using an in vitro selection method. The...
Suppression of ColE1 high-copy-number mutants by mutations in the polA gene of Escherichia coli.
We isolated three Escherichia coli suppressor strains that reduce the copy number of a mutant ColE1 high-copy-number plasmid. These mutations lower the copy number of the mutant plasmid in vivo up to...
Effect of altering GATC sequences in the plasmid ColE1 primer promoter.
Patnaik, P K, Merlin, S, Polisky, B
Plasmid ColE1 has three recognition sites for the Escherichia coli DNA adenine methylase in the immediate upstream region of the primer promoter. Two of these sites are conserved among all plasmid...
Regulated expression by readthrough translation from a plasmid-encoded beta-galactosidase.
O'Farrell, P, Polisky, B, Gelfand, D H
We have characterized expression of beta-galactosidase from a plasmid cloning vehicle, pBGP120, which carries most of the lacZ gene and contains a single EcoRI site near the end of lacZ. In addition,...
Transformation of Paramecium by microinjection of a cloned serotype gene.
Godiska, R, Aufderheide, K J, Gilley, D, Hendrie, P, Fitzwater, T, Preer, L B, ...
Paramecia of a given serotype express only one of several possible surface proteins called immobilization antigens (i-antigens). A 16-kilobase plasmid containing the gene for immobilization antigen A...
Structural analysis of RNA molecules involved in plasmid copy number control.
DNA replication of the plasmid ColE1 and its relatives is controlled mainly by a small plasmid-encoded transcript known as RNA11. We have studied the conformation of RNA1 molecules from two related...
Wong, E M, Muesing, M A, Polisky, B
We have isolated two mutant plasmid derivatives of ColE1 that exhibit temperature-sensitive replication properties. Both mutants have a normal copy number at 30 degrees C but increase their copy...
Cesareni, G, Muesing, M A, Polisky, B
A 600-base-pair region essential for ColE1 and pMBl plasmid replication contains two promoters responsible for the synthesis of two RNA molecules central to copy number control. One promoter directs...
Multilevel regulation of surface antigen gene expression in Paramecium tetraurelia.
Gilley, D, Rudman, B M, Preer, J R, Polisky, B
A family of genes is responsible for production of surface antigenic components of Paramecium tetraurelia. These surface proteins are expressed in a mutually exclusive manner. Individuals rarely...
Gilley, D, Preer, J R, Aufderheide, K J, Polisky, B
Paramecium tetraurelia can be transformed by microinjection of cloned serotype A gene sequences into the macronucleus. Transformants are detected by their ability to express serotype A surface...
Isolation and characterization of ColE1-derived plasmid copy-number mutant.
Gelfand, D H, Shepard, H M, O'Farrell, P H, Polisky, B
The plasmid pBGP120 is a ColE1 derivative that contains elements of the Escherichia coli lac operon and the Tn3 transposon. We have selected and isolated a copy-number mutant of pBGP120. In...
RNA1 is a small, plasmid-encoded transcript involved in the replication control of the plasmid ColE1. RNA1 blocks replication by preventing processing of the primer RNA necessary for the initiation...
A plasmid cloning vehicle allowing regulated expression of eukaryotic DNA in bacteria.
Polisky, B, Bishop, R J, Gelfand, D H
We have constructed a plasmid cloning vehicle in which transcription of inserted heterologous DNA fragments can be regulated by a defined bacterial operator and promoter. The lambda plac 5 EcoRIDNA...
Location of histones on simian virus 40 DNA.
The physical location of histone molecules in a simian virus 40 DNA-histone complex isolated from purified virions was examined using site-specific restriction endonucleases. The complex contains...
Specificity of substrate recognition by the EcoRI restriction endonuclease.
Polisky, B, Greene, P, Garfin, D E, McCarthy, B J, Goodman, H M, Boyer, H W
The substrate specificity of the EcoRI restriction endonuclease can be varied in vitro by changing the pH and the ionic environment of the reaction. Phosphodiester bond cleavage occurs at a DNA...
Conditional high copy number ColE1 mutants: resistance to RNA1 inhibition in vivo and in vitro.
Fitzwater, T, Zhang, X Y, Elble, R, Polisky, B
We describe three independently isolated copy number mutants of a plasmid ColE1 derivative which undergo temperature- and growth-phase-dependent DNA amplification in Escherichia coli. These mutants...
Polisky, B, Zhang, X Y, Fitzwater, T
The control of plasmid ColE1 copy number is mediated by the kinetics of interaction of two complementary plasmid-encoded RNAs. One RNA is the primer precursor and the other is a small...
Mutants Affecting Processing of DNA in Macronuclear Development in Paramecium
Rudman, B., Preer, L. B., Polisky, B., Preer-Jr., J. R.
In Paramecium tetraurelia, stock 51, the A surface protein is coded by the wild type A(51) gene, present in micronuclei in two copies and in macronuclei in about 1500 copies. DNA processing,...
Jessop-Murray, H., Martin, L. D., Gilley, D., Preer-Jr., J. R., Polisky, B.
The mutant Paramecium tetraurelia cell line d48 is unable to express the serotype A protein on its surface. Although the A gene is intact in the micronuclei of d48, the A gene copies in the...
Identification of DNA Segments Capable of Rescuing a Non-Mendelian Mutant in Paramecium
Kim, C. S., Preer-Jr, J. R., Polisky, B.
The non-Mendelian mutant d48 of Paramecium tetraurelia contains micronuclear wild type A genes, but at autogamy and conjugation proper processing fails and new macronuclei lack A genes. When cloned A...
In vitro selection of RNAs that bind to the human immunodeficiency virus type-1 gag polyprotein.
Lochrie, M A, Waugh, S, Pratt, D G, Clever, J, Parslow, T G, Polisky, B
RNA ligands that bind to the human immunodeficiency virus type-1 (HIV-1) gag polyprotein with 10(-9) M affinity were isolated from a complex pool of RNAs using an in vitro selection method. The...
Suppression of ColE1 high-copy-number mutants by mutations in the polA gene of Escherichia coli.
We isolated three Escherichia coli suppressor strains that reduce the copy number of a mutant ColE1 high-copy-number plasmid. These mutations lower the copy number of the mutant plasmid in vivo up to...
Effect of altering GATC sequences in the plasmid ColE1 primer promoter.
Patnaik, P K, Merlin, S, Polisky, B
Plasmid ColE1 has three recognition sites for the Escherichia coli DNA adenine methylase in the immediate upstream region of the primer promoter. Two of these sites are conserved among all plasmid...
Regulated expression by readthrough translation from a plasmid-encoded beta-galactosidase.
O'Farrell, P, Polisky, B, Gelfand, D H
We have characterized expression of beta-galactosidase from a plasmid cloning vehicle, pBGP120, which carries most of the lacZ gene and contains a single EcoRI site near the end of lacZ. In addition,...
Transformation of Paramecium by microinjection of a cloned serotype gene.
Godiska, R, Aufderheide, K J, Gilley, D, Hendrie, P, Fitzwater, T, Preer, L B, ...
Paramecia of a given serotype express only one of several possible surface proteins called immobilization antigens (i-antigens). A 16-kilobase plasmid containing the gene for immobilization antigen A...
Structural analysis of RNA molecules involved in plasmid copy number control.
DNA replication of the plasmid ColE1 and its relatives is controlled mainly by a small plasmid-encoded transcript known as RNA11. We have studied the conformation of RNA1 molecules from two related...
Wong, E M, Muesing, M A, Polisky, B
We have isolated two mutant plasmid derivatives of ColE1 that exhibit temperature-sensitive replication properties. Both mutants have a normal copy number at 30 degrees C but increase their copy...
Cesareni, G, Muesing, M A, Polisky, B
A 600-base-pair region essential for ColE1 and pMBl plasmid replication contains two promoters responsible for the synthesis of two RNA molecules central to copy number control. One promoter directs...
Multilevel regulation of surface antigen gene expression in Paramecium tetraurelia.
Gilley, D, Rudman, B M, Preer, J R, Polisky, B
A family of genes is responsible for production of surface antigenic components of Paramecium tetraurelia. These surface proteins are expressed in a mutually exclusive manner. Individuals rarely...
Gilley, D, Preer, J R, Aufderheide, K J, Polisky, B
Paramecium tetraurelia can be transformed by microinjection of cloned serotype A gene sequences into the macronucleus. Transformants are detected by their ability to express serotype A surface...
Isolation and characterization of ColE1-derived plasmid copy-number mutant.
Gelfand, D H, Shepard, H M, O'Farrell, P H, Polisky, B
The plasmid pBGP120 is a ColE1 derivative that contains elements of the Escherichia coli lac operon and the Tn3 transposon. We have selected and isolated a copy-number mutant of pBGP120. In...
RNA1 is a small, plasmid-encoded transcript involved in the replication control of the plasmid ColE1. RNA1 blocks replication by preventing processing of the primer RNA necessary for the initiation...
A plasmid cloning vehicle allowing regulated expression of eukaryotic DNA in bacteria.
Polisky, B, Bishop, R J, Gelfand, D H
We have constructed a plasmid cloning vehicle in which transcription of inserted heterologous DNA fragments can be regulated by a defined bacterial operator and promoter. The lambda plac 5 EcoRIDNA...
Location of histones on simian virus 40 DNA.
The physical location of histone molecules in a simian virus 40 DNA-histone complex isolated from purified virions was examined using site-specific restriction endonucleases. The complex contains...
Specificity of substrate recognition by the EcoRI restriction endonuclease.
Polisky, B, Greene, P, Garfin, D E, McCarthy, B J, Goodman, H M, Boyer, H W
The substrate specificity of the EcoRI restriction endonuclease can be varied in vitro by changing the pH and the ionic environment of the reaction. Phosphodiester bond cleavage occurs at a DNA...
Conditional high copy number ColE1 mutants: resistance to RNA1 inhibition in vivo and in vitro.
Fitzwater, T, Zhang, X Y, Elble, R, Polisky, B
We describe three independently isolated copy number mutants of a plasmid ColE1 derivative which undergo temperature- and growth-phase-dependent DNA amplification in Escherichia coli. These mutants...
Polisky, B, Zhang, X Y, Fitzwater, T
The control of plasmid ColE1 copy number is mediated by the kinetics of interaction of two complementary plasmid-encoded RNAs. One RNA is the primer precursor and the other is a small...
Mutants Affecting Processing of DNA in Macronuclear Development in Paramecium
Rudman, B., Preer, L. B., Polisky, B., Preer-Jr., J. R.
In Paramecium tetraurelia, stock 51, the A surface protein is coded by the wild type A(51) gene, present in micronuclei in two copies and in macronuclei in about 1500 copies. DNA processing,...
Jessop-Murray, H., Martin, L. D., Gilley, D., Preer-Jr., J. R., Polisky, B.
The mutant Paramecium tetraurelia cell line d48 is unable to express the serotype A protein on its surface. Although the A gene is intact in the micronuclei of d48, the A gene copies in the...
Identification of DNA Segments Capable of Rescuing a Non-Mendelian Mutant in Paramecium
Kim, C. S., Preer-Jr, J. R., Polisky, B.
The non-Mendelian mutant d48 of Paramecium tetraurelia contains micronuclear wild type A genes, but at autogamy and conjugation proper processing fails and new macronuclei lack A genes. When cloned A...