Bart J. Currie

Dengue and climate change in Australia: predictions for the future should incorporate knowledge from the past (2009)

Russell, Richard C., Currie, Bart J., Lindsay, Michael D., Mackenzie, John S., Ritchie, Scott A., Whelan, Peter I.

•Dengue transmission in Australia is currently restricted to Queensland, where the vector mosquito Aedes aegypti is established. Locally acquired infections have been reported only from urban areas...

Genomic islands from five strains of Burkholderia pseudomallei (2008)

Tuanyok, Apichai, Leadem, Benjamin R, Auerbach, Raymond K, Beckstrom-Sternberg, Stephen M, Beckstrom-Sternberg, James S, Mayo, Mark, ...

Abstract Background Burkholderia pseudomallei is the etiologic agent of melioidosis, a significant cause of morbidity and mortality where this infection is endemic. Genomic differences among strains...

Life Cycle.................................................................................................................................................................269 (2008)

Shelley F. Walton, Bart J. Currie

Background..............................................................................................................................................................268

Burkholderia pseudomalleigenome plasticity associated with genomic island variation (2008)

Tumapa, Sarinna, Holden, Matthew TG, Vesaratchavest, Mongkol, Wuthiekanun, Vanaporn, Limmathurotsakul, Direk, Chierakul, Wirongrong, ...

Abstract Background Burkholderia pseudomallei is a soil-dwelling saprophyte and the cause of melioidosis. Horizontal gene transfer contributes to the genetic diversity of this pathogen and may be an...

Recovery of a Burkholderia thailandensis-like isolate from an Australian water source (2008)

Gee, Jay E, Glass, Mindy B, Novak, Ryan T, Gal, Daniel, Mayo, Mark J, Steigerwalt, Arnold G, ...

Abstract Background Burkholderia thailandensis , a close relative of Burkholderia pseudomallei , has previously been reported only from Southeast Asia and North America. It is biochemically...

A change of heart: the new infective endocarditis prophylaxis guidelines (2008)

Daly, Christopher George, Currie, Bart J., Jeyasingham, M. S., Moulds, R. F. W., Smith, J. A., Strathmore, N. F., ...

C.G. Daly, B.J. Currie, M.S. Jeyasingham, R.F.W. Moulds, J.A. Smith, N.F. Strathmore, A.C. Street, A.N. Goss

A change of heart: the new infective endocarditis prophylaxis guidelines (2008)

Daly, Christopher George, Currie, Bart J., Jeyasingham, M. S., Moulds, R. F. W., Smith, J. A., Strathmore, N. F., ...

C.G. Daly, B.J. Currie, M.S. Jeyasingham, R.F.W. Moulds, J.A. Smith, N.F. Strathmore, A.C. Street, A.N. Goss

Current use of Australian snake antivenoms and frequency of immediate-type hypersensitivity reactions and anaphylaxis (2008)

Isbister, Geoffrey K., Brown, Simon G. A., MacDonald, Ellen, White, Julian, Currie, Bart J.

Geoffrey K. Isbister, Simon G. Brown, Ellen MacDonald, Julian White and Bart J. Currie, for the Australian Snakebite Project Investigators

Current use of Australian snake antivenoms and frequency of immediate-type hypersensitivity reactions and anaphylaxis (2008)

Isbister, Geoffrey K., Brown, Simon G. A., MacDonald, Ellen, White, Julian, Currie, Bart J.

Geoffrey K. Isbister, Simon G. Brown, Ellen MacDonald, Julian White and Bart J. Currie, for the Australian Snakebite Project Investigators

Using BOX-PCR to exclude a clonal outbreak of melioidosis (2007)

Currie, Bart J, Gal, Daniel, Mayo, Mark, Ward, Linda, Godoy, Daniel, Spratt, Brian G, ...

Abstract Background Although melioidosis in endemic regions is usually caused by a diverse range of Burkholderia pseudomallei strains, clonal outbreaks from contaminated potable water have been...

Tandem repeat regions within the Burkholderia pseudomalleigenome and their application for high resolution genotyping (2007)

U'Ren, Jana M, Schupp, James M, Pearson, Talima, Hornstra, Heidie, Friedman, Christine, Smith, Kimothy L, ...

Abstract Background The facultative, intracellular bacterium Burkholderia pseudomallei is the causative agent of melioidosis, a serious infectious disease of humans and animals. We identified and...

Use of a Single-Nucleotide Polymorphism Genotyping System to Demonstrate the Unique Epidemiology of Methicillin-Resistant Staphylococcus Aureus in Remote Aboriginal Communities (2006)

McDonald, Malcolm, Dougall, Annette, Holt, Deborah, Huygens, Flavia, Oppendisano, Frances, Giffard, Philip M., ...

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged as a major public health problem in Australia, as in many other parts of the world. High rates of CA-MRSA skin and...

Use of a Single-Nucleotide Polymorphism Genotyping System to Demonstrate the Unique Epidemiology of Methicillin-Resistant Staphylococcus Aureus in Remote Aboriginal Communities (2006)

McDonald, Malcolm, Dougall, Annette, Holt, Deborah, Huygens, Flavia, Oppendisano, Frances, Giffard, Phillip M., ...

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged as a major public health problem in Australia, as in many other parts of the world. High rates of CA-MRSA skin and...

Use of a Single-Nucleotide Polymorphism Genotyping System to Demonstrate the Unique Epidemiology of Methicillin-Resistant Staphylococcus Aureus in Remote Aboriginal Communities (2006)

McDonald, Malcolm, Dougall, Annette, Holt, Deborah, Huygens, Flavia, Oppendisano, Frances, Giffard, Philip M., ...

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged as a major public health problem in Australia, as in many other parts of the world. High rates of CA-MRSA skin and...

Use of a Single-Nucleotide Polymorphism Genotyping System to Demonstrate the Unique Epidemiology of Methicillin-Resistant Staphylococcus Aureus in Remote Aboriginal Communities (2006)

McDonald, Malcolm, Dougall, Annette, Holt, Deborah, Huygens, Flavia, Oppendisano, Frances, Giffard, Philip M., ...

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged as a major public health problem in Australia, as in many other parts of the world. High rates of CA-MRSA skin and...

Use of a Single-Nucleotide Polymorphism Genotyping System to Demonstrate the Unique Epidemiology of Methicillin-Resistant Staphylococcus Aureus in Remote Aboriginal Communities (2006)

McDonald, Malcolm, Dougall, Annette, Holt, Deborah, Huygens, Flavia, Oppendisano, Frances, Giffard, Philip M., ...

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged as a major public health problem in Australia, as in many other parts of the world. High rates of CA-MRSA skin and...

Use of a Single-Nucleotide Polymorphism Genotyping System to Demonstrate the Unique Epidemiology of Methicillin-Resistant Staphylococcus Aureus in Remote Aboriginal Communities (2006)

McDonald, Malcolm, Dougall, Annette, Holt, Deborah, Huygens, Flavia, Oppendisano, Frances, Giffard, Philip M., ...

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged as a major public health problem in Australia, as in many other parts of the world. High rates of CA-MRSA skin and...

Use of a Single-Nucleotide Polymorphism Genotyping System to Demonstrate the Unique Epidemiology of Methicillin-Resistant Staphylococcus Aureus in Remote Aboriginal Communities (2006)

McDonald, Malcolm, Dougall, Annette, Holt, Deborah, Huygens, Flavia, Oppendisano, Frances, Giffard, Philip M., ...

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged as a major public health problem in Australia, as in many other parts of the world. High rates of CA-MRSA skin and...

Use of a Single-Nucleotide Polymorphism Genotyping System to Demonstrate the Unique Epidemiology of Methicillin-Resistant Staphylococcus Aureus in Remote Aboriginal Communities (2006)

McDonald, Malcolm, Dougall, Annette, Holt, Deborah, Huygens, Flavia, Oppendisano, Frances, Giffard, Philip M., ...

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged as a major public health problem in Australia, as in many other parts of the world. High rates of CA-MRSA skin and...

Extreme weather events and environmental contamination are associated with case-clusters of melioidosis in the Northern Territory of Australia (2006)

Cheng, Allen C, Jacups, Susan P, Gal, Daniel, Mayo, Mark, Currie, Bart J

Background Melioidosis, the infection due to the environmental organism Burkholderia pseudomallei, is endemic to northern Australia and South East Asia. It is associated with exposure to mud and...

Scabies mite inactivated serine protease paralogues are present both internally in the mite gut and externally in feces (2006)

Willis, Charlene, Fischer, Katja, Walton, Shelly F., Currie, Bart J., Kemp, David J.

The scabies mite, Sarcoptes scabiei, is the causative agent of scabies, a disease that is common among disadvantaged populations and facilitates streptococcal infections with serious sequelae....

Use of a Single-Nucleotide Polymorphism Genotyping System to Demonstrate the Unique Epidemiology of Methicillin-Resistant Staphylococcus Aureus in Remote Aboriginal Communities (2006)

McDonald, Malcolm, Dougall, Annette, Holt, Deborah, Huygens, Flavia, Oppendisano, Frances, Giffard, Philip M., ...

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged as a major public health problem in Australia, as in many other parts of the world. High rates of CA-MRSA skin and...

Extreme weather events and environmental contamination are associated with case-clusters of melioidosis in the Northern Territory of Australia (2005)

Cheng, Allen C., Jacups, Susan P., Gal, Daniel, Mayo, Mark, Currie, Bart J.

Background Melioidosis, the infection due to the environmental organism Burkholderia pseudomallei, is endemic to northern Australia and South East Asia. It is associated with exposure to mud and...

A Model of Immunity to Burkholderia pseudomallei: Unique Responses following Immunization and Acute Lethal Infection (2005)

Ulett, Glen Charles, Labrooy, Justin T., Currie, Bart J., Barnes, Jodie L., Ketheesan, Natkunam

Burkholderia pseudomallei, the etiological agent of melioidosis, causes significant mortality in endemic regions, but little is known regarding the immune mechanisms required for successful...

A Model of Immunity to Burkholderia pseudomallei: Unique Responses following Immunization and Acute Lethal Infection (2005)

Ulett, Glen Charles, Labrooy, Justin T., Currie, Bart J., Barnes, Jodie L., Ketheesan, Natkunam

Burkholderia pseudomallei, the etiological agent of melioidosis, causes significant mortality in endemic regions, but little is known regarding the immune mechanisms required for successful...

Streptococcal necrotising fasciitis from diverse strains of Streptococcus pyogenesin tropical northern Australia: case series and comparison with the literature (2004)

Hassell, Marilyn, Fagan, Peter, Carson, Phillip, Currie, Bart J

Abstract Background Since the mid-1980's there has been a worldwide resurgence of severe disease from group A streptococcus (GAS), with clonal clusters implicated in Europe and the United States....

Circulating IgE in Patients with Ordinary and Crusted Scabies (2004)

Larry G. Arlian, Marjorie S. Morgan, Stephen A. Estes, Shelley F. Walton, David J. Kemp, Bart J. Currie

Serum from seven patients with ordinary scabies and six with crusted scabies were screened by immunoblotting for IgE- and IgG-specific proteins in an extract of the mite, Sarcoptes scabiei variety...

Burkholderia pseudomallei Virulence: Definition, Stability and Association with Clonality (2001)

Ulett, Glen Charles, Currie, Bart J., Clair, Timothy W., Mayo, Mark, Ketheesan, Natkunam, Labrooy, Justin, ...

Clinical presentations of melioidosis, caused by Burkholderia pseudomallei are protean, but the mechanisms underlying development of the different forms of disease remain poorly understood. In murine...

Burkholderia pseudomallei Virulence: Definition, Stability and Association with Clonality (2001)

Ulett, Glen Charles, Currie, Bart J., Clair, Timothy W., Mayo, Mark, Ketheesan, Natkunam, Labrooy, Justin, ...

Clinical presentations of melioidosis, caused by Burkholderia pseudomallei are protean, but the mechanisms underlying development of the different forms of disease remain poorly understood. In murine...

Spinal Cord Disease Due To Melioidosis (1999)

Bartley, Paul P., Pender, Michael P., Woods, Marion L., Walker, Duncan, Douglas, James A., Allworth, Anthony M., ...

Acute leioidosis typically presents as severe pneumonia or septicaemia. Woods et al have described neurological presentations of melioidosis affecting the brainstem and affecting motor weakness. We...

Spinal Cord Disease Due To Melioidosis (1999)

Bartley, Paul P., Pender, Michael P., Woods, Marion L., Walker, Duncan, Douglas, James A., Allworth, Anthony M., ...

Acute leioidosis typically presents as severe pneumonia or septicaemia. Woods et al have described neurological presentations of melioidosis affecting the brainstem and affecting motor weakness. We...

Spinal Cord Disease Due To Melioidosis (1999)

Bartley, Paul P., Pender, Michael P., Woods, Marion L., Walker, Duncan, Douglas, James A., Allworth, Anthony M., ...

Acute leioidosis typically presents as severe pneumonia or septicaemia. Woods et al have described neurological presentations of melioidosis affecting the brainstem and affecting motor weakness. We...

Field Evaluation of the ICT Malaria P.f/P.v Immunochromatographic Test for Detection of Plasmodium falciparum and Plasmodium vivax in Patients with a Presumptive Clinical Diagnosis of Malaria in Eastern Indonesia

Tjitra, Emiliana, Suprianto, Sri, Dyer, Mary, Currie, Bart J., Anstey, Nicholas M.

In areas such as eastern Indonesia where both Plasmodium falciparum and Plasmodium vivax occur, rapid antigen detection tests for malaria need to be able to detect both species. We evaluated the new...

Persistent ICT Malaria P.f/P.v Panmalarial and HRP2 Antigen Reactivity after Treatment of Plasmodium falciparum Malaria Is Associated with Gametocytemia and Results in False-Positive Diagnoses of Plasmodium vivax in Convalescence

Tjitra, Emiliana, Suprianto, Sri, McBroom, James, Currie, Bart J., Anstey, Nicholas M.

A problem with rapid Plasmodium falciparum-specific antigen histidine-rich protein 2 (HRP2) detection tests for malaria is the persistence of antigen in blood after the disappearance of asexual-stage...

Distribution and Antigenicity of Fibronectin Binding Proteins (SfbI and SfbII) of Streptococcus pyogenes Clinical Isolates from the Northern Territory, Australia

Goodfellow, Alison M., Hibble, Megan, Talay, Susanne R., Kreikemeyer, Bernd, Currie, Bart J., Sriprakash, Kadaba S., ...

Fibronectin binding proteins play an important role in the adherence and invasion of group A streptococci (GAS). Genotypically distinct GAS isolates were screened for the presence and expression of...

NAD-Glycohydrolase Production and speA and speC Distribution in Group A Streptococcus (GAS) Isolates Do Not Correlate with Severe GAS Diseases in the Australian Population

DelVecchio, Armando, Maley, Michael, Currie, Bart J., Sriprakash, K. S.

Streptococcus pyogenes isolates from a tropical region and a subtropical region of Australia with high and low incidences of severe streptococcal diseases, respectively, were analyzed for speA, speB,...

Community-Acquired Bacteremic Acinetobacter Pneumonia in Tropical Australia Is Caused by Diverse Strains of Acinetobacter baumannii, with Carriage in the Throat in At-Risk Groups

Anstey, Nicholas M., Currie, Bart J., Hassell, Marilyn, Palmer, Didier, Dwyer, Brian, Seifert, Harald

Acinetobacter isolates from eight subjects with community-acquired Acinetobacter pneumonia (CAAP), a major cause of fatal community-acquired pneumonia in tropical Australia, were phenotypically and...

Strains of Streptococcus pyogenes from Severe Invasive Infections Bind HEp2 and HaCaT Cells More Avidly than Strains from Uncomplicated Infections

Edwards, Mandy L, Fagan, Peter K., Smith-Vaughan, Heidi, Currie, Bart J., Sriprakash, Kadaba S.

Epidemiologically unrelated Streptococcus pyogenes strains isolated from blood, throat, and skin were assayed for adherence to HEp2 and HaCaT cells. Invasive isolates showed significantly higher...

Ethical problems of evaluating a new treatment for melioidosis

Cheng, Allen C, Lowe, Michael, Stephens, Dianne P, Currie, Bart J

When mortality from melioidosis fell sharply after multiple changes in management at an Australian hospital, doctors wanted to identify whether a new drug was responsible. But designing a trial that...

Evolution of sfbI Encoding Streptococcal Fibronectin-Binding Protein I: Horizontal Genetic Transfer and Gene Mosaic Structure

Towers, Rebecca J., Fagan, Peter K., Talay, Susanne R., Currie, Bart J., Sriprakash, Kadaba S., Walker, Mark J., ...

Streptococcal fibronectin-binding protein is an important virulence factor involved in colonization and invasion of epithelial cells and tissues by Streptococcus pyogenes. In order to investigate the...

Plasminogen Binding by Group A Streptococcal Isolates from a Region of Hyperendemicity for Streptococcal Skin Infection and a High Incidence of Invasive Infection

McKay, Fiona C., McArthur, Jason D., Sanderson-Smith, Martina L., Gardam, Sandra, Currie, Bart J., Sriprakash, Kadaba S., ...

Reports of resurgence in invasive group A streptococcal (GAS) infections come mainly from affluent populations with infrequent exposure to GAS. In the Northern Territory (NT) of Australia, high...

Outcomes of Patients with Melioidosis Treated with Meropenem

Cheng, Allen C., Fisher, Dale A., Anstey, Nicholas M., Stephens, Dianne P., Jacups, Susan P., Currie, Bart J.

Melioidosis, an infection due to Burkholderia pseudomallei, is endemic in southeast Asia and northern Australia. We reviewed our experience with meropenem in the treatment of severe melioidosis in 63...

Cryptococcus Infection in Tropical Australia

Jenney, Adam, Pandithage, Kishan, Fisher, Dale A., Currie, Bart J.

Eighteen cases of disease caused by the saprophytic fungi Cryptococcus neoformans and Cryptococcus bacillisporus are described from the Northern Territory of Australia. The majority of infections...

Fibronectin-Binding Protein Gene Recombination and Horizontal Transfer between Group A and G Streptococci

Towers, Rebecca J., Gal, Daniel, McMillan, David, Sriprakash, Kadaba S., Currie, Bart J., Walker, Mark J., ...

We report evidence of interspecies gene transfer between the important virulence factor genes sfbI and gfbA. Because the identified group G streptococcus gfbA types possess DNA cassettes that can be...

Isolates of Burkholderia pseudomallei from Northern Australia Are Distinct by Multilocus Sequence Typing, but Strain Types Do Not Correlate with Clinical Presentation

Cheng, Allen C., Godoy, Daniel, Mayo, Mark, Gal, Daniel, Spratt, Brian G., Currie, Bart J.

Melioidosis is the disease caused by the saprophytic organism Burkholderia pseudomallei. Previous studies have suggested some strain tropism and differential virulence. In this study, we defined...

Melioidosis: Epidemiology, Pathophysiology, and Management

Cheng, Allen C., Currie, Bart J.

Melioidosis, caused by the gram-negative saprophyte Burkholderia pseudomallei, is a disease of public health importance in southeast Asia and northern Australia that is associated with high...

Surface Analyses and Immune Reactivities of Major Cell Wall-Associated Proteins of Group A Streptococcus

Cole, Jason N., Ramirez, Ruben D., Currie, Bart J., Cordwell, Stuart J., Djordjevic, Steven P., Walker, Mark J.

A proteomic analysis was undertaken to identify cell wall-associated proteins of Streptococcus pyogenes. Seventy-four distinct cell wall-associated proteins were identified, 66 of which were novel....

Clonality and Recombination in Genetically Differentiated Subgroups of Cryptococcus gattii†

Campbell, Leona T., Currie, Bart J., Krockenberger, Mark, Malik, Richard, Meyer, Wieland, Heitman, Joseph, ...

Cryptococcus gattii is a pathogenic yeast that together with Cryptococcus neoformans causes cryptococcosis in humans and animals. High numbers of viable C. gattii propagules can be obtained from...

Development and Evaluation of a Real-Time PCR Assay Targeting the Type III Secretion System of Burkholderia pseudomallei

Novak, Ryan T., Glass, Mindy B., Gee, Jay E., Gal, Daniel, Mayo, Mark J., Currie, Bart J., ...

Here we report on the development of a discriminatory real-time assay for the rapid identification of Burkholderia pseudomallei isolates and the evaluation of this assay for sensitivity against...

Further Evaluation of a Rapid Diagnostic Test for Melioidosis in an Area of Endemicity

O'Brien, Mathew, Freeman, Kevin, Lum, Gary, Cheng, Allen C., Jacups, Susan P., Currie, Bart J.

Immunochromatographic test (ICT) kits for the rapid detection of immunoglobulin G (IgG) and IgM antibodies to Burkholderia pseudomallei were compared to the indirect hemagglutination (IHA) assay. In...

Recovering Streptococci from the Throat, a Practical Alternative to Direct Plating in Remote Tropical Communities

McDonald, Malcolm, Towers, Rebecca, Fagan, Peter, McKinnon, Melita, Benger, Norma, Andrews, Ross, ...

Throat swabs are regarded as the “gold standard” for diagnosing streptococcal pharyngitis and for surveillance research. Culturing throats in remote tropical settings is logistically difficult,...

Use of a Variable Amplicon Typing Scheme Reveals Considerable Variation in the Accessory Genomes of Isolates of Burkholderia pseudomallei†

Duangsonk, Kwanjit, Gal, Daniel, Mayo, Mark, Hart, C. Anthony, Currie, Bart J., Winstanley, Craig

Melioidosis, a disease caused by the bacterium Burkholderia pseudomallei, is endemic in southeast Asia and northern Australia. We used suppression subtractive hybridization (SSH) to identify...

Nonrandom Distribution of Burkholderia pseudomallei Clones in Relation to Geographical Location and Virulence

Vesaratchavest, Mongkol, Tumapa, Sarinna, Day, Nicholas P. J., Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Holden, Matthew T. G., ...

Burkholderia pseudomallei is a soil-dwelling saprophyte and the causative agent of melioidosis, a life-threatening human infection. Most cases are reported from northeast Thailand and northern...

Clinical Evaluation of a Type III Secretion System Real-Time PCR Assay for Diagnosing Melioidosis

Meumann, Ella M., Novak, Ryan T., Gal, Daniel, Kaestli, Mirjam E., Mayo, Mark, Hanson, Joshua P., ...

A Burkholderia pseudomallei type III secretion system real-time PCR assay was evaluated on clinical specimens in a region where melioidosis is endemic. The PCR was positive in 30/33 (91%) patients...

Field Evaluation of the ICT Malaria P.f/P.v Immunochromatographic Test for Detection of Plasmodium falciparum and Plasmodium vivax in Patients with a Presumptive Clinical Diagnosis of Malaria in Eastern Indonesia

Tjitra, Emiliana, Suprianto, Sri, Dyer, Mary, Currie, Bart J., Anstey, Nicholas M.

In areas such as eastern Indonesia where both Plasmodium falciparum and Plasmodium vivax occur, rapid antigen detection tests for malaria need to be able to detect both species. We evaluated the new...

Persistent ICT Malaria P.f/P.v Panmalarial and HRP2 Antigen Reactivity after Treatment of Plasmodium falciparum Malaria Is Associated with Gametocytemia and Results in False-Positive Diagnoses of Plasmodium vivax in Convalescence

Tjitra, Emiliana, Suprianto, Sri, McBroom, James, Currie, Bart J., Anstey, Nicholas M.

A problem with rapid Plasmodium falciparum-specific antigen histidine-rich protein 2 (HRP2) detection tests for malaria is the persistence of antigen in blood after the disappearance of asexual-stage...

Distribution and Antigenicity of Fibronectin Binding Proteins (SfbI and SfbII) of Streptococcus pyogenes Clinical Isolates from the Northern Territory, Australia

Goodfellow, Alison M., Hibble, Megan, Talay, Susanne R., Kreikemeyer, Bernd, Currie, Bart J., Sriprakash, Kadaba S., ...

Fibronectin binding proteins play an important role in the adherence and invasion of group A streptococci (GAS). Genotypically distinct GAS isolates were screened for the presence and expression of...

NAD-Glycohydrolase Production and speA and speC Distribution in Group A Streptococcus (GAS) Isolates Do Not Correlate with Severe GAS Diseases in the Australian Population

DelVecchio, Armando, Maley, Michael, Currie, Bart J., Sriprakash, K. S.

Streptococcus pyogenes isolates from a tropical region and a subtropical region of Australia with high and low incidences of severe streptococcal diseases, respectively, were analyzed for speA, speB,...

Community-Acquired Bacteremic Acinetobacter Pneumonia in Tropical Australia Is Caused by Diverse Strains of Acinetobacter baumannii, with Carriage in the Throat in At-Risk Groups

Anstey, Nicholas M., Currie, Bart J., Hassell, Marilyn, Palmer, Didier, Dwyer, Brian, Seifert, Harald

Acinetobacter isolates from eight subjects with community-acquired Acinetobacter pneumonia (CAAP), a major cause of fatal community-acquired pneumonia in tropical Australia, were phenotypically and...

Strains of Streptococcus pyogenes from Severe Invasive Infections Bind HEp2 and HaCaT Cells More Avidly than Strains from Uncomplicated Infections

Edwards, Mandy L, Fagan, Peter K., Smith-Vaughan, Heidi, Currie, Bart J., Sriprakash, Kadaba S.

Epidemiologically unrelated Streptococcus pyogenes strains isolated from blood, throat, and skin were assayed for adherence to HEp2 and HaCaT cells. Invasive isolates showed significantly higher...

Ethical problems of evaluating a new treatment for melioidosis

Cheng, Allen C, Lowe, Michael, Stephens, Dianne P, Currie, Bart J

When mortality from melioidosis fell sharply after multiple changes in management at an Australian hospital, doctors wanted to identify whether a new drug was responsible. But designing a trial that...

Evolution of sfbI Encoding Streptococcal Fibronectin-Binding Protein I: Horizontal Genetic Transfer and Gene Mosaic Structure

Towers, Rebecca J., Fagan, Peter K., Talay, Susanne R., Currie, Bart J., Sriprakash, Kadaba S., Walker, Mark J., ...

Streptococcal fibronectin-binding protein is an important virulence factor involved in colonization and invasion of epithelial cells and tissues by Streptococcus pyogenes. In order to investigate the...

Plasminogen Binding by Group A Streptococcal Isolates from a Region of Hyperendemicity for Streptococcal Skin Infection and a High Incidence of Invasive Infection

McKay, Fiona C., McArthur, Jason D., Sanderson-Smith, Martina L., Gardam, Sandra, Currie, Bart J., Sriprakash, Kadaba S., ...

Reports of resurgence in invasive group A streptococcal (GAS) infections come mainly from affluent populations with infrequent exposure to GAS. In the Northern Territory (NT) of Australia, high...

Outcomes of Patients with Melioidosis Treated with Meropenem

Cheng, Allen C., Fisher, Dale A., Anstey, Nicholas M., Stephens, Dianne P., Jacups, Susan P., Currie, Bart J.

Melioidosis, an infection due to Burkholderia pseudomallei, is endemic in southeast Asia and northern Australia. We reviewed our experience with meropenem in the treatment of severe melioidosis in 63...

Further Evaluation of a Rapid Diagnostic Test for Melioidosis in an Area of Endemicity

O'Brien, Mathew, Freeman, Kevin, Lum, Gary, Cheng, Allen C., Jacups, Susan P., Currie, Bart J.

Immunochromatographic test (ICT) kits for the rapid detection of immunoglobulin G (IgG) and IgM antibodies to Burkholderia pseudomallei were compared to the indirect hemagglutination (IHA) assay. In...

Cryptococcus Infection in Tropical Australia

Jenney, Adam, Pandithage, Kishan, Fisher, Dale A., Currie, Bart J.

Eighteen cases of disease caused by the saprophytic fungi Cryptococcus neoformans and Cryptococcus bacillisporus are described from the Northern Territory of Australia. The majority of infections...

Fibronectin-Binding Protein Gene Recombination and Horizontal Transfer between Group A and G Streptococci

Towers, Rebecca J., Gal, Daniel, McMillan, David, Sriprakash, Kadaba S., Currie, Bart J., Walker, Mark J., ...

We report evidence of interspecies gene transfer between the important virulence factor genes sfbI and gfbA. Because the identified group G streptococcus gfbA types possess DNA cassettes that can be...

Isolates of Burkholderia pseudomallei from Northern Australia Are Distinct by Multilocus Sequence Typing, but Strain Types Do Not Correlate with Clinical Presentation

Cheng, Allen C., Godoy, Daniel, Mayo, Mark, Gal, Daniel, Spratt, Brian G., Currie, Bart J.

Melioidosis is the disease caused by the saprophytic organism Burkholderia pseudomallei. Previous studies have suggested some strain tropism and differential virulence. In this study, we defined...

Melioidosis: Epidemiology, Pathophysiology, and Management

Cheng, Allen C., Currie, Bart J.

Melioidosis, caused by the gram-negative saprophyte Burkholderia pseudomallei, is a disease of public health importance in southeast Asia and northern Australia that is associated with high...

Surface Analyses and Immune Reactivities of Major Cell Wall-Associated Proteins of Group A Streptococcus

Cole, Jason N., Ramirez, Ruben D., Currie, Bart J., Cordwell, Stuart J., Djordjevic, Steven P., Walker, Mark J.

A proteomic analysis was undertaken to identify cell wall-associated proteins of Streptococcus pyogenes. Seventy-four distinct cell wall-associated proteins were identified, 66 of which were novel....

Clonality and Recombination in Genetically Differentiated Subgroups of Cryptococcus gattii†

Campbell, Leona T., Currie, Bart J., Krockenberger, Mark, Malik, Richard, Meyer, Wieland, Heitman, Joseph, ...

Cryptococcus gattii is a pathogenic yeast that together with Cryptococcus neoformans causes cryptococcosis in humans and animals. High numbers of viable C. gattii propagules can be obtained from...

Development and Evaluation of a Real-Time PCR Assay Targeting the Type III Secretion System of Burkholderia pseudomallei

Novak, Ryan T., Glass, Mindy B., Gee, Jay E., Gal, Daniel, Mayo, Mark J., Currie, Bart J., ...

Here we report on the development of a discriminatory real-time assay for the rapid identification of Burkholderia pseudomallei isolates and the evaluation of this assay for sensitivity against...

Recovering Streptococci from the Throat, a Practical Alternative to Direct Plating in Remote Tropical Communities

McDonald, Malcolm, Towers, Rebecca, Fagan, Peter, McKinnon, Melita, Benger, Norma, Andrews, Ross, ...

Throat swabs are regarded as the “gold standard” for diagnosing streptococcal pharyngitis and for surveillance research. Culturing throats in remote tropical settings is logistically difficult,...

Use of a Variable Amplicon Typing Scheme Reveals Considerable Variation in the Accessory Genomes of Isolates of Burkholderia pseudomallei†

Duangsonk, Kwanjit, Gal, Daniel, Mayo, Mark, Hart, C. Anthony, Currie, Bart J., Winstanley, Craig

Melioidosis, a disease caused by the bacterium Burkholderia pseudomallei, is endemic in southeast Asia and northern Australia. We used suppression subtractive hybridization (SSH) to identify...

Nonrandom Distribution of Burkholderia pseudomallei Clones in Relation to Geographical Location and Virulence

Vesaratchavest, Mongkol, Tumapa, Sarinna, Day, Nicholas P. J., Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Holden, Matthew T. G., ...

Burkholderia pseudomallei is a soil-dwelling saprophyte and the causative agent of melioidosis, a life-threatening human infection. Most cases are reported from northeast Thailand and northern...

Clinical Evaluation of a Type III Secretion System Real-Time PCR Assay for Diagnosing Melioidosis

Meumann, Ella M., Novak, Ryan T., Gal, Daniel, Kaestli, Mirjam E., Mayo, Mark, Hanson, Joshua P., ...

A Burkholderia pseudomallei type III secretion system real-time PCR assay was evaluated on clinical specimens in a region where melioidosis is endemic. The PCR was positive in 30/33 (91%) patients...

Use of a Single-Nucleotide Polymorphism Genotyping System To Demonstrate the Unique Epidemiology of Methicillin-Resistant Staphylococcus aureus in Remote Aboriginal Communities

McDonald, Malcolm, Dougall, Annette, Holt, Deborah, Huygens, Flavia, Oppedisano, Frances, Giffard, Philip M., ...

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged as a major public health problem in Australia, as in many other parts of the world. High rates of CA-MRSA skin and...

Spinal Cord Disease Due To Melioidosis

Bartley, Paul P., Pender, Michael P., Woods, Marion L., Walker, Duncan, Douglas, James A., Allworth, Anthony M., ...

Acute leioidosis typically presents as severe pneumonia or septicaemia. Woods et al have described neurological presentations of melioidosis affecting the brainstem and affecting motor weakness. We...

Sensitive and Specific Molecular Detection of Burkholderia pseudomallei, the Causative Agent of Melioidosis, in the Soil of Tropical Northern Australia▿

Kaestli, Mirjam, Mayo, Mark, Harrington, Glenda, Watt, Felicity, Hill, Jason, Gal, Daniel, ...

Burkholderia pseudomallei, the cause of the severe disease melioidosis in humans and animals, is a gram-negative saprophyte living in soil and water of areas of endemicity such as tropical northern...

Accuracy of Burkholderia pseudomallei Identification Using the API 20NE System and a Latex Agglutination Test▿

Amornchai, Premjit, Chierakul, Wirongrong, Wuthiekanun, Vanaporn, Mahakhunkijcharoen, Yuvadee, Phetsouvanh, Rattanaphone, Currie, Bart J., ...

In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei...

Problems in Diagnosing Scabies, a Global Disease in Human and Animal Populations

Walton, Shelley F., Currie, Bart J.

Scabies is a worldwide disease and a major public health problem in many developing countries, related primarily to poverty and overcrowding. In remote Aboriginal communities in northern Australia,...

A Horizontal Gene Transfer Event Defines Two Distinct Groups within Burkholderia pseudomallei That Have Dissimilar Geographic Distributions▿ †

Tuanyok, Apichai, Auerbach, Raymond K., Brettin, Thomas S., Bruce, David C., Munk, A. Christine, Detter, J. Chris, ...

Burkholderia pseudomallei is the etiologic agent of melioidosis. Many disease manifestations are associated with melioidosis, and the mechanisms causing this variation are unknown; genomic...

Genetic Diversity of Burkholderia pseudomallei Isolates in Australia▿

Cheng, Allen C., Ward, Linda, Godoy, Daniel, Norton, Robert, Mayo, Mark, Gal, Daniel, ...

Melioidosis is caused by the gram-negative saprophytic bacterium Burkholderia pseudomallei, which is endemic to southeast Asia and northern Australia. We have previously found evidence of geographic...

Prevalence and Sequence Diversity of a Factor Required for Actin-Based Motility in Natural Populations of Burkholderia Species▿ †

Sitthidet, Chayada, Stevens, Joanne M., Chantratita, Narisara, Currie, Bart J., Peacock, Sharon J., Korbsrisate, Sunee, ...

Actin-based motility of the melioidosis pathogen Burkholderia pseudomallei requires BimA. We report a high degree of conservation of bimA in 99 B. pseudomallei isolates from the area of endemicity. A...

Landscape Changes Influence the Occurrence of the Melioidosis Bacterium Burkholderia pseudomallei in Soil in Northern Australia

Kaestli, Mirjam, Mayo, Mark, Harrington, Glenda, Ward, Linda, Watt, Felicity, Hill, Jason V., ...

Melioidosis is a severe disease affecting humans and animals in the tropics. It is caused by the bacterium Burkholderia pseudomallei, which lives in tropical soil and especially occurs in southeast...

Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis

Currie, Bart J., Haslem, Asha, Pearson, Talima, Hornstra, Heidie, Leadem, Benjamin, Mayo, Mark, ...

One-sentence summary for table of contents: This analysis can identify a clonal outbreak of this disease within 8 hours of receipt of bacterial isolates.

A Regional Initiative to Reduce Skin Infections amongst Aboriginal Children Living in Remote Communities of the Northern Territory, Australia

Andrews, Ross M., Kearns, Therese, Connors, Christine, Parker, Colin, Carville, Kylie, Currie, Bart J., ...

Skin infections are endemic in many in remote Australian Aboriginal communities and have been linked to very high rates of chronic heart and kidney disease in this population. We report the results...