Olivier, Jean Paul, Raabe, Thomas, Henkerneyer, Mark, Dickson, Barry, Mbamalu, Geraldine, Margolis, Ben, ...
A Drosophila gene (drk) encodes a widely expressed protein with a single SH2 domain and two flanking SH3 domains, which is homologous to the Sem-5 protein of C. elegans and mammalian GRB2. Genetic...
Olivier, Jean Paul, Raabe, Thomas, Henkerneyer, Mark, Dickson, Barry, Mbamalu, Geraldine, Margolis, Ben, ...
A Drosophila gene (drk) encodes a widely expressed protein with a single SH2 domain and two flanking SH3 domains, which is homologous to the Sem-5 protein of C. elegans and mammalian GRB2. Genetic...
Straight, Samuel W., Chen, Liguang, Karnak, David, Margolis, Ben
To investigate the targeting mechanism for proteins bound to the mammalian Lin-7 (mLin-7) PDZ domain, we created receptor protein chimeras composed of the carboxyl-terminal amino acids of LET-23...
Lee, Seonok, Fan, Shuling, Makarova, Olya, Straight, Samuel, Margolis, Ben
Mammalian Lin-2 (mLin-2)/CASK is a membrane-associated guanylate kinase (MAGUK) and contains multidomain modules that mediate protein-protein interactions important for the establishment and...
Calderwood, David A., Fujioka, Yosuke, De Pereda, Jose M., García-Alvarez, Begoña, Nakamoto, Tetsuya, Margolis, Ben, ...
The cytoplasmic domains (tails) of heterodimeric integrin adhesion receptors mediate integrins' biological functions by binding to cytoplasmic proteins. Most integrin β tails contain one or two...
Loss of PALS1 Expression Leads to Tight Junction and Polarity Defects
Straight, Samuel W., Shin, Kunyoo, Fogg, Vanessa C., Fan, Shuling, Liu, Chia-Jen, Roh, Michael, ...
Prior work in our laboratory established a connection between the PALS1/PATJ/CRB3 and Par6/Par3/aPKC protein complexes at the tight junction of mammalian epithelial cells. Utilizing a stable small...
Structural basis for L27 domain-mediated assembly of signaling and cell polarity complexes
Li, Yuanhe, Karnak, David, Demeler, Borries, Margolis, Ben, Lavie, Arnon
L27 is a protein-binding domain that can assemble essential proteins for signaling and cell polarity into complexes by interacting in a heterodimeric manner. One of these protein complexes is the...
Khanna, Hemant, Hurd, Toby W., Lillo, Concepcion, Shu, Xinhua, Parapuram, Sunil K., He, Shirley, ...
Mutations in the retinitis pigmentosa GTPase regulator (RPGR) gene account for almost 20% of patients with retinitis pigmentosa. Most mutations are detected in alternatively-spliced RPGR-ORF15...
Straight, Samuel W., Chen, Liguang, Karnak, David, Margolis, Ben
To investigate the targeting mechanism for proteins bound to the mammalian Lin-7 (mLin-7) PDZ domain, we created receptor protein chimeras composed of the carboxyl-terminal amino acids of LET-23...
Lee, Seonok, Fan, Shuling, Makarova, Olya, Straight, Samuel, Margolis, Ben
Mammalian Lin-2 (mLin-2)/CASK is a membrane-associated guanylate kinase (MAGUK) and contains multidomain modules that mediate protein-protein interactions important for the establishment and...
Calderwood, David A., Fujioka, Yosuke, De Pereda, Jose M., García-Alvarez, Begoña, Nakamoto, Tetsuya, Margolis, Ben, ...
The cytoplasmic domains (tails) of heterodimeric integrin adhesion receptors mediate integrins' biological functions by binding to cytoplasmic proteins. Most integrin β tails contain one or two...
Loss of PALS1 Expression Leads to Tight Junction and Polarity Defects
Straight, Samuel W., Shin, Kunyoo, Fogg, Vanessa C., Fan, Shuling, Liu, Chia-Jen, Roh, Michael, ...
Prior work in our laboratory established a connection between the PALS1/PATJ/CRB3 and Par6/Par3/aPKC protein complexes at the tight junction of mammalian epithelial cells. Utilizing a stable small...
Structural basis for L27 domain-mediated assembly of signaling and cell polarity complexes
Li, Yuanhe, Karnak, David, Demeler, Borries, Margolis, Ben, Lavie, Arnon
L27 is a protein-binding domain that can assemble essential proteins for signaling and cell polarity into complexes by interacting in a heterodimeric manner. One of these protein complexes is the...
PALS1 Regulates E-Cadherin Trafficking in Mammalian Epithelial Cells
Wang, Qian, Chen, Xiao-Wei, Margolis, Ben
Protein Associated with Lin Seven 1 (PALS1) is an evolutionarily conserved scaffold protein that targets to the tight junction in mammalian epithelia. Prior work in our laboratory demonstrated that...
PATJ regulates directional migration of mammalian epithelial cells
Shin, Kunyoo, Wang, Qian, Margolis, Ben
Directional migration is important in wound healing by epithelial cells. Recent studies have shown that polarity proteins such as mammalian Partitioning-defective 6 (Par6), atypical protein kinase C...
A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin β interactions
Fan, Shuling, Fogg, Vanessa, Wang, Qian, Chen, Xiao-Wei, Liu, Chia-Jen, Margolis, Ben
The Crumbs family of apical transmembrane proteins regulates apicobasal polarity via protein interactions with a conserved C-terminal sequence, ERLI. However, one of the mammalian Crumbs proteins,...
PATJ regulates tight junction formation and polarity in mammalian epithelial cells
Shin, Kunyoo, Straight, Sam, Margolis, Ben
Recent studies have revealed an important role for tight junction protein complexes in epithelial cell polarity. One of these complexes contains the apical transmembrane protein, Crumbs, and two...
Roh, Michael H., Makarova, Olga, Liu, Chia-Jen, Shin, Lee, Seonok, Laurinec, Stephanie, ...
Membrane-associated guanylate kinase (Maguk) proteins are scaffold proteins that contain PSD-95–Discs Large–zona occludens-1 (PDZ), Src homology 3, and guanylate kinase domains. A subset of Maguk...
Cargo of Kinesin Identified as Jip Scaffolding Proteins and Associated Signaling Molecules
Verhey, Kristen J., Meyer, Debra, Deehan, Reneé, Blenis, John, Schnapp, Bruce J., Rapoport, Tom A., ...
The cargo that the molecular motor kinesin moves along microtubules has been elusive. We searched for binding partners of the COOH terminus of kinesin light chain, which contains tetratricopeptide...