Bernt Eric Uhlin

Novel role for a bacterial nucleoid protein in translation of mRNAs with suboptimal ribosome-binding sites (2010)

Park, Hyun-Sook, Östberg, Yngve, Johansson, Jörgen, Wagner, E. Gerhart H., Uhlin, Bernt Eric

In Escherichia coli, the major nucleoid protein H-NS limits transcription by acting as a repressor or transcriptional silencer, presumably by its ability to close the looped chromosome domains in the...

BioMed Central (2009)

Bmc Microbiology, Barbro Lindmark, Pramod Kumar Rompikuntal, Karolis Vaitkevicius, Tianyan Song, Yoshimitsu Mizunoe, ...

Research article Outer membrane vesicle-mediated release of cytolethal distending toxin (CDT) from Campylobacter jejuni

Release of the type I secreted α-haemolysin via outer membrane vesicles from Escherichia coli (2006)

Balsalobre, Carlos, Silván, José Manuel, Berglund, Stina, Mizunoe, Yoshimitsu, Uhlin, Bernt Eric, Nyunt Wai, Sun

The α-haemolysin is an important virulence factor commonly expressed by extraintestinal pathogenic Escherichia coli. The secretion of the α-haemolysin is mediated by the type I secretion system and...

Physical Properties of Escherichia coli P Pili Measured by Optical Tweezers (2004)

Jass, Jana, Schedin, Staffan, Fällman, Erik, Ohlsson, Jörgen, Nilsson, Ulf J, Uhlin, Bernt Eric, ...

The mechanical behavior of individual P pili of uropathogenic Escherichia coli has been investigated using optical tweezers. P pili, whose main part constitutes the PapA rod, composed of 103 PapA...

Differential protease-mediated turnover of H-NS and StpA revealed by a mutation altering protein stability and stationary-phase survival of Escherichia coli

Johansson, Jörgen, Uhlin, Bernt Eric

The Escherichia coli proteins H-NS is recognized as an important component among the major nucleoid-associated proteins. In studies of E. coli strains with defects in H-NS, we discovered a mutant...

Alterations in Protein Expression Caused by the hha Mutation in Escherichia coli: Influence of Growth Medium Osmolarity

Balsalobre, Carlos, Johansson, Jörgen, Uhlin, Bernt Eric, Juárez, Antonio, Muñoa, Francisco J.

The Hha protein belongs to a new family of regulators involved in the environmental regulation of virulence factors. The aim of this work was to study the effect of the hha mutation on the overall...

Silencing and Activation of ClyA Cytotoxin Expression in Escherichia coli

Westermark, Marie, Oscarsson, Jan, Mizunoe, Yoshimitsu, Urbonaviciene, Jurate, Uhlin, Bernt Eric

Cytolysin A (ClyA) is a pore-forming cytotoxic protein encoded by the clyA gene of Escherichia coli K-12. Genetic analysis suggested that clyA is silenced by the nucleoid protein H-NS. Purified H-NS...

Heteromeric Interactions among Nucleoid-Associated Bacterial Proteins: Localization of StpA-Stabilizing Regions in H-NS of Escherichia coli

Johansson, Jörgen, Eriksson, Sven, Sondén, Berit, Wai, Sun Nyunt, Uhlin, Bernt Eric

The nucleoid-associated proteins H-NS and StpA in Escherichia coli bind DNA as oligomers and are implicated in gene regulatory systems. There is evidence for both homomeric and heteromeric...

Cytocidal and Apoptotic Effects of the ClyA Protein from Escherichia coli on Primary and Cultured Monocytes and Macrophages

Lai, Xin-He, Arencibia, Ignacio, Johansson, Anders, Wai, Sun Nyunt, Oscarsson, Jan, Kalfas, Sotos, ...

Cytolysin A (ClyA) is a newly discovered cytolytic protein of Escherichia coli K-12 that mediates a hemolytic phenotype. We show here that highly purified ClyA and ClyA-expressing E. coli were...

H-NS and StpA Proteins Stimulate Expression of the Maltose Regulon in Escherichia coli

Johansson, Jörgen, Dagberg, Björn, Richet, Evelyne, Uhlin, Bernt Eric

The nucleoid-associated protein H-NS is a major component of the chromosome-protein complex, and it is known to influence the regulation of many genes in Escherichia coli. Its role in gene regulation...

Characterization of a Pore-Forming Cytotoxin Expressed by Salmonella enterica Serovars Typhi and Paratyphi A

Oscarsson, Jan, Westermark, Marie, Löfdahl, Sven, Olsen, Björn, Palmgren, Helena, Mizunoe, Yoshimitsu, ...

Cytolysin A (ClyA) is a pore-forming cytotoxic protein encoded by the clyA gene that has been characterized so far only in Escherichia coli. Using DNA sequence analysis and PCR, we established that...

Transcriptional Analysis of the sfa Determinant Revealing Multiple mRNA Processing Events in the Biogenesis of S Fimbriae in Pathogenic Escherichia coli

Balsalobre, Carlos, Morschhäuser, Joachim, Jass, Jana, Hacker, Jörg, Uhlin, Bernt Eric

Among the virulence factors present in pathogenic extraintestinal Escherichia coli strains, expression of fimbrial adhesins is necessary for attachment to the host tissues and subsequent...

Characterization of Dominantly Negative Mutant ClyA Cytotoxin Proteins in Escherichia coli

Wai, Sun Nyunt, Westermark, Marie, Oscarsson , Jan, Jass , Jana, Maier, Elke, Benz, Roland, ...

We report studies of the subcellular localization of the ClyA cytotoxic protein and of mutations causing defective translocation to the periplasm in Escherichia coli. The ability of ClyA to...

Regulatory cross-talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein

Xia, Yan, Gally, David, Forsman-Semb, Kristina, Uhlin, Bernt Eric

Pathogenic Escherichia coli often carry determinants for several different adhesins. We show a direct communication between two adhesin gene clusters in uropathogenic E.coli: type 1 fimbriae (fim)...

Physical Properties of Escherichia coli P Pili Measured by Optical Tweezers

Jass, Jana, Schedin, Staffan, Fällman, Erik, Ohlsson, Jörgen, Nilsson, Ulf J., Uhlin, Bernt Eric, ...

The mechanical behavior of individual P pili of uropathogenic Escherichia coli has been investigated using optical tweezers. P pili, whose main part constitutes the PapA rod, composed of ∼103 PapA...

Active Cytotoxic Necrotizing Factor 1 Associated with Outer Membrane Vesicles from Uropathogenic Escherichia coli

Kouokam, J. Clavin, Wai, Sun Nyunt, Fällman, Maria, Dobrindt, Ulrich, Hacker, Jörg, Uhlin, Bernt Eric

Cytotoxic necrotizing factor type 1 (CNF1) is one of the virulence factors produced by uropathogenic Escherichia coli (UPEC). How this toxin is translocated from the bacterial cytoplasm to the...

Role of Histone-Like Proteins H-NS and StpA in Expression of Virulence Determinants of Uropathogenic Escherichia coli

Müller, Claudia M., Dobrindt, Ulrich, Nagy, Gábor, Emödy, Levente, Uhlin, Bernt Eric, Hacker, Jörg

The histone-like protein H-NS is a global regulator in Escherichia coli that has been intensively studied in nonpathogenic strains. However, no comprehensive study on the role of H-NS and its...

Cyclic AMP-Dependent Osmoregulation of crp Gene Expression in Escherichia coli

Balsalobre, Carlos, Johansson, Jörgen, Uhlin, Bernt Eric

We have found that the cyclic AMP (cAMP) receptor protein (CRP)-cAMP regulatory complex in Escherichia coli is subject to osmoregulation at the level of crp gene expression. This osmoregulation was...

Differential protease-mediated turnover of H-NS and StpA revealed by a mutation altering protein stability and stationary-phase survival of Escherichia coli

Johansson, Jörgen, Uhlin, Bernt Eric

The Escherichia coli proteins H-NS is recognized as an important component among the major nucleoid-associated proteins. In studies of E. coli strains with defects in H-NS, we discovered a mutant...

Alterations in Protein Expression Caused by the hha Mutation in Escherichia coli: Influence of Growth Medium Osmolarity

Balsalobre, Carlos, Johansson, Jörgen, Uhlin, Bernt Eric, Juárez, Antonio, Muñoa, Francisco J.

The Hha protein belongs to a new family of regulators involved in the environmental regulation of virulence factors. The aim of this work was to study the effect of the hha mutation on the overall...

Silencing and Activation of ClyA Cytotoxin Expression in Escherichia coli

Westermark, Marie, Oscarsson, Jan, Mizunoe, Yoshimitsu, Urbonaviciene, Jurate, Uhlin, Bernt Eric

Cytolysin A (ClyA) is a pore-forming cytotoxic protein encoded by the clyA gene of Escherichia coli K-12. Genetic analysis suggested that clyA is silenced by the nucleoid protein H-NS. Purified H-NS...

Heteromeric Interactions among Nucleoid-Associated Bacterial Proteins: Localization of StpA-Stabilizing Regions in H-NS of Escherichia coli

Johansson, Jörgen, Eriksson, Sven, Sondén, Berit, Wai, Sun Nyunt, Uhlin, Bernt Eric

The nucleoid-associated proteins H-NS and StpA in Escherichia coli bind DNA as oligomers and are implicated in gene regulatory systems. There is evidence for both homomeric and heteromeric...

Cytocidal and Apoptotic Effects of the ClyA Protein from Escherichia coli on Primary and Cultured Monocytes and Macrophages

Lai, Xin-He, Arencibia, Ignacio, Johansson, Anders, Wai, Sun Nyunt, Oscarsson, Jan, Kalfas, Sotos, ...

Cytolysin A (ClyA) is a newly discovered cytolytic protein of Escherichia coli K-12 that mediates a hemolytic phenotype. We show here that highly purified ClyA and ClyA-expressing E. coli were...

H-NS and StpA Proteins Stimulate Expression of the Maltose Regulon in Escherichia coli

Johansson, Jörgen, Dagberg, Björn, Richet, Evelyne, Uhlin, Bernt Eric

The nucleoid-associated protein H-NS is a major component of the chromosome-protein complex, and it is known to influence the regulation of many genes in Escherichia coli. Its role in gene regulation...

Characterization of a Pore-Forming Cytotoxin Expressed by Salmonella enterica Serovars Typhi and Paratyphi A

Oscarsson, Jan, Westermark, Marie, Löfdahl, Sven, Olsen, Björn, Palmgren, Helena, Mizunoe, Yoshimitsu, ...

Cytolysin A (ClyA) is a pore-forming cytotoxic protein encoded by the clyA gene that has been characterized so far only in Escherichia coli. Using DNA sequence analysis and PCR, we established that...

Transcriptional Analysis of the sfa Determinant Revealing Multiple mRNA Processing Events in the Biogenesis of S Fimbriae in Pathogenic Escherichia coli

Balsalobre, Carlos, Morschhäuser, Joachim, Jass, Jana, Hacker, Jörg, Uhlin, Bernt Eric

Among the virulence factors present in pathogenic extraintestinal Escherichia coli strains, expression of fimbrial adhesins is necessary for attachment to the host tissues and subsequent...

Characterization of Dominantly Negative Mutant ClyA Cytotoxin Proteins in Escherichia coli

Wai, Sun Nyunt, Westermark, Marie, Oscarsson , Jan, Jass , Jana, Maier, Elke, Benz, Roland, ...

We report studies of the subcellular localization of the ClyA cytotoxic protein and of mutations causing defective translocation to the periplasm in Escherichia coli. The ability of ClyA to...

Regulatory cross-talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein

Xia, Yan, Gally, David, Forsman-Semb, Kristina, Uhlin, Bernt Eric

Pathogenic Escherichia coli often carry determinants for several different adhesins. We show a direct communication between two adhesin gene clusters in uropathogenic E.coli: type 1 fimbriae (fim)...

Physical Properties of Escherichia coli P Pili Measured by Optical Tweezers

Jass, Jana, Schedin, Staffan, Fällman, Erik, Ohlsson, Jörgen, Nilsson, Ulf J., Uhlin, Bernt Eric, ...

The mechanical behavior of individual P pili of uropathogenic Escherichia coli has been investigated using optical tweezers. P pili, whose main part constitutes the PapA rod, composed of ∼103 PapA...

Active Cytotoxic Necrotizing Factor 1 Associated with Outer Membrane Vesicles from Uropathogenic Escherichia coli

Kouokam, J. Clavin, Wai, Sun Nyunt, Fällman, Maria, Dobrindt, Ulrich, Hacker, Jörg, Uhlin, Bernt Eric

Cytotoxic necrotizing factor type 1 (CNF1) is one of the virulence factors produced by uropathogenic Escherichia coli (UPEC). How this toxin is translocated from the bacterial cytoplasm to the...

Role of Histone-Like Proteins H-NS and StpA in Expression of Virulence Determinants of Uropathogenic Escherichia coli

Müller, Claudia M., Dobrindt, Ulrich, Nagy, Gábor, Emödy, Levente, Uhlin, Bernt Eric, Hacker, Jörg

The histone-like protein H-NS is a global regulator in Escherichia coli that has been intensively studied in nonpathogenic strains. However, no comprehensive study on the role of H-NS and its...

Cyclic AMP-Dependent Osmoregulation of crp Gene Expression in Escherichia coli

Balsalobre, Carlos, Johansson, Jörgen, Uhlin, Bernt Eric

We have found that the cyclic AMP (cAMP) receptor protein (CRP)-cAMP regulatory complex in Escherichia coli is subject to osmoregulation at the level of crp gene expression. This osmoregulation was...

A Sticky Chain Model of the Elongation and Unfolding of Escherichia coli P Pili under Stress

Andersson, Magnus, Fällman, Erik, Uhlin, Bernt Eric, Axner, Ove

A model of the elongation of P pili expressed by uropathogenic Escherichia coli exposed to stress is presented. The model is based upon the sticky chain concept, which is based upon Hooke's law for...

Dynamic Force Spectroscopy of E. coli P Pili

Andersson, Magnus, Fällman, Erik, Uhlin, Bernt Eric, Axner, Ove

Surface organelles (so-called pili) expressed on the bacterial membrane mediate the adhesion of Escherichia coli causing urinary tract infection. These pili possess some extraordinary elongation...

Regulatory Interactions among Adhesin Gene Systems of Uropathogenic Escherichia coli▿

Lindberg, Stina, Xia, Yan, Sondén, Berit, Göransson, Mikael, Hacker, Jörg, Uhlin, Bernt Eric

Uropathogenic Escherichia coli strain J96 carries multiple determinants for fimbrial adhesins. The regulatory protein PapB of P fimbriae has previously been implicated in potential coregulatory...

Proteomic Characterization of the Whole Secretome of Legionella pneumophila and Functional Analysis of Outer Membrane Vesicles▿ †

Galka, Frank, Wai, Sun Nyunt, Kusch, Harald, Engelmann, Susanne, Hecker, Michael, Schmeck, Bernd, ...

Secretion of effector molecules is one of the major mechanisms by which the intracellular human pathogen Legionella pneumophila interacts with host cells during infection. Specific secretion...

The Biomechanical Properties of E. coli Pili for Urinary Tract Attachment Reflect the Host Environment

Andersson, Magnus, Uhlin, Bernt Eric, Fällman, Erik

Uropathogenic Escherichia coli express pili that mediate binding to host tissue cells. We demonstrate with in situ force measuring optical tweezers that the ability of P and type 1 pili to elongate...

Type 1 Fimbriae, a Colonization Factor of Uropathogenic Escherichia coli, Are Controlled by the Metabolic Sensor CRP-cAMP

Müller, Claudia M., Åberg, Anna, Straseviçiene, Jurate, Emődy, Levente, Uhlin, Bernt Eric, Balsalobre, Carlos

Type 1 fimbriae are a crucial factor for the virulence of uropathogenic Escherichia coli during the first steps of infection by mediating adhesion to epithelial cells. They are also required for the...

Differential effects and interactions of endogenous and horizontally acquired H-NS-like proteins in pathogenic Escherichia coli

Müller, Claudia M, Schneider, György, Dobrindt, Ulrich, Emödy, Levente, Hacker, Jörg, Uhlin, Bernt Eric

The nucleoid-associated protein H-NS is important for gene regulation in Escherichia coli. We have studied H-NS interaction with StpA and an uncharacterized H-NS-like protein, Hfp, in the...