Binghua Shen

Construction and Initial Characterization of Escherichia coli Strains with Few or No Intact Chromosomal rRNA Operons

Asai, Tsuneaki, Condon, Ciarán, Voulgaris, Justina, Zaporojets, Dmitry, Shen, Binghua, Al-Omar, Michaal, ...

The Escherichia coli genome carries seven rRNA (rrn) operons, each containing three rRNA genes. The presence of multiple operons has been an obstacle to many studies of rRNA because the effect of...

Proteome-scale purification of human proteins from bacteria

Braun, Pascal, Hu, Yanhui, Shen, Binghua, Halleck, Allison, Koundinya, Malvika, Harlow, Ed, ...

The completion of the human genome project and the development of high-throughput approaches herald a dramatic acceleration in the pace of biological research. One of the most compelling next steps...

Building a human kinase gene repository: Bioinformatics, molecular cloning, and functional validation

Park, Jaehong, Hu, Yanhui, Murthy, T. V. S., Vannberg, Fredrik, Shen, Binghua, Rolfs, Andreas, ...

Kinases catalyze the phosphorylation of proteins, lipids, sugars, nucleosides, and other important cellular metabolites and play key regulatory roles in all aspects of eukaryotic cell physiology....

Construction and Initial Characterization of Escherichia coli Strains with Few or No Intact Chromosomal rRNA Operons

Asai, Tsuneaki, Condon, Ciarán, Voulgaris, Justina, Zaporojets, Dmitry, Shen, Binghua, Al-Omar, Michaal, ...

The Escherichia coli genome carries seven rRNA (rrn) operons, each containing three rRNA genes. The presence of multiple operons has been an obstacle to many studies of rRNA because the effect of...

Proteome-scale purification of human proteins from bacteria

Braun, Pascal, Hu, Yanhui, Shen, Binghua, Halleck, Allison, Koundinya, Malvika, Harlow, Ed, ...

The completion of the human genome project and the development of high-throughput approaches herald a dramatic acceleration in the pace of biological research. One of the most compelling next steps...

Building a human kinase gene repository: Bioinformatics, molecular cloning, and functional validation

Park, Jaehong, Hu, Yanhui, Murthy, T. V. S., Vannberg, Fredrik, Shen, Binghua, Rolfs, Andreas, ...

Kinases catalyze the phosphorylation of proteins, lipids, sugars, nucleosides, and other important cellular metabolites and play key regulatory roles in all aspects of eukaryotic cell physiology....

A Biomedically Enriched Collection of 7000 Human ORF Clones

Rolfs, Andreas, Hu, Yanhui, Ebert, Lars, Hoffmann, Dietmar, Zuo, Dongmei, Ramachandran, Niro, ...

We report the production and availability of over 7000 fully sequence verified plasmid ORF clones representing over 3400 unique human genes. These ORF clones were derived using the human MGC...

Production and sequence validation of a complete full length ORF collection for the pathogenic bacterium Vibrio cholerae

Rolfs, Andreas, Montor, Wagner R., Yoon, Sang Sun, Hu, Yanhui, Bhullar, Bhupinder, Kelley, Fontina, ...

Cholera, an infectious disease with global impact, is caused by pathogenic strains of the bacterium Vibrio cholerae. High-throughput functional proteomics technologies now offer the opportunity to...