Biswarup Mukhopadhyay

Publication List Details

Period

1987 - 2009

Number

22

Co-Authors

The complete genome sequence of Staphylothermus marinusreveals differences in sulfur metabolism among heterotrophic Crenarchaeota (2009)

Anderson, Iain J, Dharmarajan, Lakshmi, Rodriguez, Jason, Hooper, Sean, Porat, Iris, Ulrich, Luke E, ...

Abstract Background Staphylothermus marinus is an anaerobic, sulfur-reducing peptide fermenter of the archaeal phylum Crenarchaeota. It is the third heterotrophic, obligate sulfur reducing...

A novel pH2 control on the expression of flagella in the hyperthermophilic strictly hydrogenotrophic methanarchaeaon Methanococcus jannaschii

Mukhopadhyay, Biswarup, Johnson, Eric F., Wolfe, Ralph S.

The methanarchaeon, Methanococcus jannaschii, a hyperthermophilic, autotrophic, and strictly hydrogenotrophic inhabitant of submarine hydrothermal vents, was cultivated in a reactor at two hydrogen...

Conditions for Vigorous Growth on Sulfide and Reactor-Scale Cultivation Protocols for the Thermophilic Green Sulfur Bacterium Chlorobium tepidum

Mukhopadhyay, Biswarup, Johnson, Eric F., Ascano, Manuel

We describe a reactor-scale cultivation protocol for the fastest-growing and only known thermophilic member of the family Chlorobiaceae, Chlorobium tepidum. We discovered that C. tepidum would grow...

Reactor-Scale Cultivation of the Hyperthermophilic Methanarchaeon Methanococcus jannaschii to High Cell Densities

Mukhopadhyay, Biswarup, Johnson, Eric F., Wolfe, Ralph S.

For the hyperthermophilic and barophilic methanarchaeon Methanococcus jannaschii, we have developed a medium and protocols for reactor-scale cultivation that improved the final cell yield per liter...

Oxaloacetate Synthesis in the Methanarchaeon Methanosarcina barkeri: Pyruvate Carboxylase Genes and a Putative Escherichia coli-Type Bifunctional Biotin Protein Ligase Gene (bpl/birA) Exhibit a Unique Organization

Mukhopadhyay, Biswarup, Purwantini, Endang, Kreder, Cynthia L., Wolfe, Ralph S.

Evidence is presented that, in Methanosarcina barkeri oxaloacetate synthesis, an essential and major CO2 fixation reaction is catalyzed by an apparent α4β4-type acetyl coenzyme A-independent...

The Genome of M. acetivorans Reveals Extensive Metabolic and Physiological Diversity

Galagan, James E., Nusbaum, Chad, Roy, Alice, Endrizzi, Matthew G., Macdonald, Pendexter, FitzHugh, Will, ...

Methanogenesis, the biological production of methane, plays a pivotal role in the global carbon cycle and contributes significantly to global warming. The majority of methane in nature is derived...

The Phosphoenolpyruvate Carboxylase from Methanothermobacter thermautotrophicus Has a Novel Structure

Patel, Hiten M., Kraszewski, Jessica L., Mukhopadhyay, Biswarup

In Methanothermobacter thermautotrophicus, oxaloacetate synthesis is a major and essential CO2-fixation reaction. This methanogenic archaeon possesses two oxaloacetate-synthesizing enzymes, pyruvate...

A novel pH2 control on the expression of flagella in the hyperthermophilic strictly hydrogenotrophic methanarchaeaon Methanococcus jannaschii

Mukhopadhyay, Biswarup, Johnson, Eric F., Wolfe, Ralph S.

The methanarchaeon, Methanococcus jannaschii, a hyperthermophilic, autotrophic, and strictly hydrogenotrophic inhabitant of submarine hydrothermal vents, was cultivated in a reactor at two hydrogen...

Conditions for Vigorous Growth on Sulfide and Reactor-Scale Cultivation Protocols for the Thermophilic Green Sulfur Bacterium Chlorobium tepidum

Mukhopadhyay, Biswarup, Johnson, Eric F., Ascano, Manuel

We describe a reactor-scale cultivation protocol for the fastest-growing and only known thermophilic member of the family Chlorobiaceae, Chlorobium tepidum. We discovered that C. tepidum would grow...

Reactor-Scale Cultivation of the Hyperthermophilic Methanarchaeon Methanococcus jannaschii to High Cell Densities

Mukhopadhyay, Biswarup, Johnson, Eric F., Wolfe, Ralph S.

For the hyperthermophilic and barophilic methanarchaeon Methanococcus jannaschii, we have developed a medium and protocols for reactor-scale cultivation that improved the final cell yield per liter...

Oxaloacetate Synthesis in the Methanarchaeon Methanosarcina barkeri: Pyruvate Carboxylase Genes and a Putative Escherichia coli-Type Bifunctional Biotin Protein Ligase Gene (bpl/birA) Exhibit a Unique Organization

Mukhopadhyay, Biswarup, Purwantini, Endang, Kreder, Cynthia L., Wolfe, Ralph S.

Evidence is presented that, in Methanosarcina barkeri oxaloacetate synthesis, an essential and major CO2 fixation reaction is catalyzed by an apparent α4β4-type acetyl coenzyme A-independent...

The Genome of M. acetivorans Reveals Extensive Metabolic and Physiological Diversity

Galagan, James E., Nusbaum, Chad, Roy, Alice, Endrizzi, Matthew G., Macdonald, Pendexter, FitzHugh, Will, ...

Methanogenesis, the biological production of methane, plays a pivotal role in the global carbon cycle and contributes significantly to global warming. The majority of methane in nature is derived...

The Phosphoenolpyruvate Carboxylase from Methanothermobacter thermautotrophicus Has a Novel Structure

Patel, Hiten M., Kraszewski, Jessica L., Mukhopadhyay, Biswarup

In Methanothermobacter thermautotrophicus, oxaloacetate synthesis is a major and essential CO2-fixation reaction. This methanogenic archaeon possesses two oxaloacetate-synthesizing enzymes, pyruvate...

Establishment of a Gene Expression System in Ochrobactrum anthropi

Seleem, Mohamed N., Ali, Mohammed, Boyle, Stephen M., Mukhopadhyay, Biswarup, Witonsky, Sharon G., Schurig, Gerhardt G., ...

Genetic studies of Ochrobactrum anthropi are hindered by the lack of a suitable gene expression system. We constructed a set of vectors containing several promoters and a His tag fusion in the N...

Identification of Pyruvate Carboxylase Genes in Pseudomonas aeruginosa PAO1 and Development of a P. aeruginosa-Based Overexpression System for α4- and α4β4-Type Pyruvate Carboxylases▿

Lai, Huafang, Kraszewski, Jessica L., Purwantini, Endang, Mukhopadhyay, Biswarup

Pyruvate carboxylase (PYC) is an ecologically, medically, and industrially important enzyme. It is widespread in all three domains of life, the archaea, bacteria, and eukarya. PYC catalyzes...

Genome Sequence of Thermofilum pendens Reveals an Exceptional Loss of Biosynthetic Pathways without Genome Reduction▿ †

Anderson, Iain, Rodriguez, Jason, Susanti, Dwi, Porat, Iris, Reich, Claudia, Ulrich, Luke E., ...

We report the complete genome of Thermofilum pendens, a deeply branching, hyperthermophilic member of the order Thermoproteales in the archaeal kingdom Crenarchaeota. T. pendens is a...

Coenzyme F420-Dependent Sulfite Reductase-Enabled Sulfite Detoxification and Use of Sulfite as a Sole Sulfur Source by Methanococcus maripaludis▿

Johnson, Eric F., Mukhopadhyay, Biswarup

Coenzyme F420-dependent sulfite reductase (Fsr) of Methanocaldococcus jannaschii, a sulfite-tolerant methanogen, was expressed with activity in Methanococcus maripaludis, a sulfite-sensitive...

Conversion of NO2 to NO by reduced coenzyme F420 protects mycobacteria from nitrosative damage

Purwantini, Endang, Mukhopadhyay, Biswarup

In mycobacteria, F420, a deazaflavin derivative, acts as a hydride transfer coenzyme for an F420-specific glucose-6-phosphate dehydrogenase (Fgd). Physiologically relevant reactions in the...

TmpL, a Transmembrane Protein Required for Intracellular Redox Homeostasis and Virulence in a Plant and an Animal Fungal Pathogen

Kim, Kwang-Hyung, Willger, Sven D., Park, Sang-Wook, Puttikamonkul, Srisombat, Grahl, Nora, Cho, Yangrae, ...

The regulation of intracellular levels of reactive oxygen species (ROS) is critical for developmental differentiation and virulence of many pathogenic fungi. In this report we demonstrate that a...