BORIS STRIEPEN

Forward Genetic Analysis of the Apicomplexan Cell Division Cycle in Toxoplasma gondii (2008)

Marc-Jan Gubbels, Margaret Lehmann, Mani Muthalagi, Maria E. Jerome, Carrie F. Brooks, Tomasz Szatanek, ...

Apicomplexa are obligate intracellular pathogens that have fine-tuned their proliferative strategies to match a large variety of host cells. A critical aspect of this adaptation is a flexible cell...

Building the Perfect Parasite: Cell Division in Apicomplexa (2007)

Boris Striepen, Carly N. Jordan, Sarah Reiff

Apicomplexans are pathogens responsible for malaria, toxoplasmosis, and crytposporidiosis in humans, and a wide range of livestock diseases. These unicellular eukaryotes are stealthy invaders,...

IMP dehydrogenase from the protozoan parasite Toxoplasma gondii (2005)

William J. Sullivan, Stacy E. Dixon, Catherine Li, Boris Striepen, Sherry F. Queener

The opportunistic apicomplexan parasite Toxoplasma gondii damages fetuses in utero and threatens immunocompromised individuals. The toxicity associated with standard antitoxoplasmal therapies, which...

Plastid-Targeting Peptides from the Chlorarachniophyte Bigelowiella natans (2004)

MATTHEW B. ROGERS, JOHN M. ARCHIBALD, MATTHEW A. FIELD, CATHERINE LI, BORIS STRIEPEN, PATRICK J. KEELING

Chlorarachniophytes are marine amoeboflagellate protists that have acquired their plastid (chloroplast) through secondary endosymbiosis with a green alga. Like other algae, most of the proteins...

Nuclear-encoded proteins target to the plastid in Toxoplasma gondii and Plasmodium falciparum

Waller, Ross F., Keeling, Patrick J., Donald, Robert G. K., Striepen, Boris, Handman, Emanuela, Lang-Unnasch, Naomi, ...

A vestigial, nonphotosynthetic plastid has been identified recently in protozoan parasites of the phylum Apicomplexa. The apicomplexan plastid, or “apicoplast,” is indispensable, but the complete...

Daughter Cell Assembly in the Protozoan Parasite Toxoplasma gondii

Hu, Ke, Mann, Tara, Striepen, Boris, Beckers, Con J. M., Roos, David S., Murray, John M.

The phylum Apicomplexa includes thousands of species of obligate intracellular parasites, many of which are significant human and/or animal pathogens. Parasites in this phylum replicate by assembling...

Genetic complementation in apicomplexan parasites

Striepen, Boris, White, Michael W., Li, Catherine, Guerini, Michael N., Malik, S.-Banoo, Logsdon, John M., ...

A robust forward genetic model for Apicomplexa could greatly enhance functional analysis of genes in these important protozoan pathogens. We have developed and successfully tested a genetic...

A plastid segregation defect in the protozoan parasite Toxoplasma gondii

He, Cynthia Y., Shaw, Michael K., Pletcher, Charles H., Striepen, Boris, Tilney, Lewis G., Roos, David S.

Apicomplexan parasites—including the causative agents of malaria (Plasmodium sp.) and toxoplasmosis (Toxoplasma gondii)—harbor a secondary endosymbiotic plastid, acquired by lateral genetic...

High-Throughput Growth Assay for Toxoplasma gondii Using Yellow Fluorescent Protein

Gubbels, Marc-Jan, Li, Catherine, Striepen, Boris

A high-throughput growth assay for the protozoan parasite Toxoplasma gondii was developed based on a highly fluorescent transgenic parasite line. These parasites are stably transfected with a tandem...

Gene transfer in the evolution of parasite nucleotide biosynthesis

Striepen, Boris, Pruijssers, Andrea J. P., Huang, Jinling, Li, Catherine, Gubbels, Marc-Jan, Umejiego, Nwakaso N., ...

Nucleotide metabolic pathways provide numerous successful targets for antiparasitic chemotherapy, but the human pathogen Cryptosporidium parvum thus far has proved extraordinarily refractory to...

Sarcocystis neurona Merozoites Express a Family of Immunogenic Surface Antigens That Are Orthologues of the Toxoplasma gondii Surface Antigens (SAGs) and SAG-Related Sequences†

Howe, Daniel K., Gaji, Rajshekhar Y., Mroz-Barrett, Meaghan, Gubbels, Marc-Jan, Striepen, Boris, Stamper, Shelby

Sarcocystis neurona is a member of the Apicomplexa that causes myelitis and encephalitis in horses but normally cycles between the opossum and small mammals. Analysis of an S. neurona expressed...

Class I Major Histocompatibility Complex Presentation of Antigens That Escape from the Parasitophorous Vacuole of Toxoplasma gondii

Gubbels, Marc-Jan, Striepen, Boris, Shastri, Nilabh, Turkoz, Mustafa, Robey, Ellen A.

The intracellular parasite Toxoplasma gondii, the causative agent of toxoplasmosis, induces a protective CD8 T-cell response in its host; however, the mechanisms by which T. gondii proteins are...

IMP Dehydrogenase from the Protozoan Parasite Toxoplasma gondii

Sullivan, William J., Dixon, Stacy E., Li, Catherine, Striepen, Boris, Queener, Sherry F.

The opportunistic apicomplexan parasite Toxoplasma gondii damages fetuses in utero and threatens immunocompromised individuals. The toxicity associated with standard antitoxoplasmal therapies, which...

CD40 induces macrophage anti–Toxoplasma gondii activity by triggering autophagy-dependent fusion of pathogen-containing vacuoles and lysosomes

Andrade, Rosa M., Wessendarp, Matthew, Gubbels, Marc-Jan, Striepen, Boris, Subauste, Carlos S.

Many intracellular pathogens, including Toxoplasma gondii, survive within macrophages by residing in vacuoles that avoid fusion with lysosomes. It is important to determine whether cell-mediated...

Nuclear-encoded proteins target to the plastid in Toxoplasma gondii and Plasmodium falciparum

Waller, Ross F., Keeling, Patrick J., Donald, Robert G. K., Striepen, Boris, Handman, Emanuela, Lang-Unnasch, Naomi, ...

A vestigial, nonphotosynthetic plastid has been identified recently in protozoan parasites of the phylum Apicomplexa. The apicomplexan plastid, or “apicoplast,” is indispensable, but the complete...

Daughter Cell Assembly in the Protozoan Parasite Toxoplasma gondii

Hu, Ke, Mann, Tara, Striepen, Boris, Beckers, Con J. M., Roos, David S., Murray, John M.

The phylum Apicomplexa includes thousands of species of obligate intracellular parasites, many of which are significant human and/or animal pathogens. Parasites in this phylum replicate by assembling...

Genetic complementation in apicomplexan parasites

Striepen, Boris, White, Michael W., Li, Catherine, Guerini, Michael N., Malik, S.-Banoo, Logsdon, John M., ...

A robust forward genetic model for Apicomplexa could greatly enhance functional analysis of genes in these important protozoan pathogens. We have developed and successfully tested a genetic...

A plastid segregation defect in the protozoan parasite Toxoplasma gondii

He, Cynthia Y., Shaw, Michael K., Pletcher, Charles H., Striepen, Boris, Tilney, Lewis G., Roos, David S.

Apicomplexan parasites—including the causative agents of malaria (Plasmodium sp.) and toxoplasmosis (Toxoplasma gondii)—harbor a secondary endosymbiotic plastid, acquired by lateral genetic...

High-Throughput Growth Assay for Toxoplasma gondii Using Yellow Fluorescent Protein

Gubbels, Marc-Jan, Li, Catherine, Striepen, Boris

A high-throughput growth assay for the protozoan parasite Toxoplasma gondii was developed based on a highly fluorescent transgenic parasite line. These parasites are stably transfected with a tandem...

Gene transfer in the evolution of parasite nucleotide biosynthesis

Striepen, Boris, Pruijssers, Andrea J. P., Huang, Jinling, Li, Catherine, Gubbels, Marc-Jan, Umejiego, Nwakaso N., ...

Nucleotide metabolic pathways provide numerous successful targets for antiparasitic chemotherapy, but the human pathogen Cryptosporidium parvum thus far has proved extraordinarily refractory to...

Sarcocystis neurona Merozoites Express a Family of Immunogenic Surface Antigens That Are Orthologues of the Toxoplasma gondii Surface Antigens (SAGs) and SAG-Related Sequences†

Howe, Daniel K., Gaji, Rajshekhar Y., Mroz-Barrett, Meaghan, Gubbels, Marc-Jan, Striepen, Boris, Stamper, Shelby

Sarcocystis neurona is a member of the Apicomplexa that causes myelitis and encephalitis in horses but normally cycles between the opossum and small mammals. Analysis of an S. neurona expressed...

Class I Major Histocompatibility Complex Presentation of Antigens That Escape from the Parasitophorous Vacuole of Toxoplasma gondii

Gubbels, Marc-Jan, Striepen, Boris, Shastri, Nilabh, Turkoz, Mustafa, Robey, Ellen A.

The intracellular parasite Toxoplasma gondii, the causative agent of toxoplasmosis, induces a protective CD8 T-cell response in its host; however, the mechanisms by which T. gondii proteins are...

IMP Dehydrogenase from the Protozoan Parasite Toxoplasma gondii

Sullivan, William J., Dixon, Stacy E., Li, Catherine, Striepen, Boris, Queener, Sherry F.

The opportunistic apicomplexan parasite Toxoplasma gondii damages fetuses in utero and threatens immunocompromised individuals. The toxicity associated with standard antitoxoplasmal therapies, which...

CD40 induces macrophage anti–Toxoplasma gondii activity by triggering autophagy-dependent fusion of pathogen-containing vacuoles and lysosomes

Andrade, Rosa M., Wessendarp, Matthew, Gubbels, Marc-Jan, Striepen, Boris, Subauste, Carlos S.

Many intracellular pathogens, including Toxoplasma gondii, survive within macrophages by residing in vacuoles that avoid fusion with lysosomes. It is important to determine whether cell-mediated...

Apicoplast fatty acid synthesis is essential for organelle biogenesis and parasite survival in Toxoplasma gondii

Mazumdar, Jolly, H. Wilson, Emma, Masek, Kate, A. Hunter, Christopher, Striepen, Boris

Apicomplexan parasites are the cause of numerous important human diseases including malaria and AIDS-associated opportunistic infections. Drug treatment for these diseases is not satisfactory and is...

Mining the Plasmodium genome database to define organellar function: what does the apicoplast do?

Roos, David S, Crawford, Michael J, Donald, Robert G K, Fraunholz, Martin, Harb, Omar S, He, Cynthia Y, ...

Apicomplexan species constitute a diverse group of parasitic protozoa, which are responsible for a wide range of diseases in many organisms. Despite differences in the diseases they cause, these...

Building the Perfect Parasite: Cell Division in Apicomplexa

Striepen, Boris, Jordan, Carly N, Reiff, Sarah, Van Dooren, Giel G

Apicomplexans are pathogens responsible for malaria, toxoplasmosis, and crytposporidiosis in humans, and a wide range of livestock diseases. These unicellular eukaryotes are stealthy invaders,...

Forward Genetic Analysis of the Apicomplexan Cell Division Cycle in Toxoplasma gondii

Gubbels, Marc-Jan, Lehmann, Margaret, Muthalagi, Mani, Jerome, Maria E, Brooks, Carrie F, Szatanek, Tomasz, ...

Apicomplexa are obligate intracellular pathogens that have fine-tuned their proliferative strategies to match a large variety of host cells. A critical aspect of this adaptation is a flexible cell...

The Plastid of Toxoplasma gondii Is Divided by Association with the Centrosomes

Striepen, Boris, Crawford, Michael J., Shaw, Michael K., Tilney, Lewis G., Seeber, Frank, Roos, David S.

Apicomplexan parasites harbor a single nonphotosynthetic plastid, the apicoplast, which is essential for parasite survival. Exploiting Toxoplasma gondii as an accessible system for cell biological...

Toxoplasma gondii Tic20 is essential for apicoplast protein import

Van Dooren, Giel G., Tomova, Cveta, Agrawal, Swati, Humbel, Bruno M., Striepen, Boris

Apicomplexan parasites harbor a secondary plastid that has lost the ability to photosynthesize yet is essential for the parasite to multiply and cause disease. Bioinformatic analyses predict that...