Leptihn, Sebastian, Har, Jia, Chen, Jianzhu, Ho, Bow, Wohland, Thorsten, Ding, Jeak
Abstract Background Antimicrobial peptides are found in all kingdoms of life. During the evolution of multicellular organisms, antimicrobial peptides were established as key elements of innate...
Perumal Samy R, Pachiappan A, Gopalakrishnakone P, Thwin Maung, Hian Yap, Chow Vincent, ...
Abstract Background Burkholderia pseudomallei are the causative agent of melioidosis. Increasing resistance of the disease to antibiotics is a severe problem in treatment regime and has led to...
Zhu, Yong, Ng, Patricia M. L., Wang, Lihui, Ho, Bow, Ding, Jeak Ling
Carbohydrate-binding lectins play essential roles as pattern recognition receptors in innate immunity in both vertebrates and invertebrates. The carcinolectins 5 (CL5a and CL5b, the CL5 isoforms of...
Zhu, Yong, Ng, Patricia M. L., Wang, Lihui, Ho, Bow, Ding, Jeak Ling
Carbohydrate-binding lectins play essential roles as pattern recognition receptors in innate immunity in both vertebrates and invertebrates. The carcinolectins 5 (CL5a and CL5b, the CL5 isoforms of...
Tandem repeats of Sushi3 peptide with enhanced LPS-binding and -neutralizing activities (2003)
Li, Changgui, Ng, Miang Lon Patricia, Zhu, Yong, Ho, Bow, Ding, Jeak Ling
Endotoxin, also known as lipopolysaccharide (LPS), is the major mediator of septic shock due to Gram‐negative bacterial infection. Chemically synthesized S3 peptide, derived from Sushi3 domain...
Engineering a novel secretion signal for cross-host recombinant protein expression (2002)
Tan, Nguan Soon, Ho, Bow, Ding, Jeak Ling
Protein secretion is conferred by a hydrophobic secretion signal usually located at the N-terminal of the polypeptide. We report here, the identification of a novel secretion signal (SS) that is...
Monteiro, Mario A., Zheng, Peng-yuan, Ho, Bow, Yokota, Shin-ichi, Amano, Ken-ichi, Pan, Zhi-jun, ...
Past studies have shown that the cell surface lipopolysaccharides (LPSs) of the ubiquitous human gastric pathogen Helicobacter pylori (a type 1 carcinogen) isolated from people residing in Europe and...
Sein W. Hla, Kok P. Hui, Wan C. Tan, Bow Ho
The respiratory tracts of bronchiectasis patients may be persistently colonized with Pseudomonas aeruginosa, despite intensive chemotherapy. The organism may undergo phenotypic changes in these...
Yau, Yin Hoe, Ho, Bow, Tan, Nguan Soon, Ng, Miang Lon, Ding, Jeak Ling
Factor C protein isolated from the horseshoe crab, Carcinoscorpius rotundicauda, has endotoxin binding capability. Synthetic peptides of 34 amino acids based on the sequence of two regions of factor...
A Novel Fluorescent Protein-Based Biosensor for Gram-Negative Bacteria
Goh, Yan Y., Ho, Bow, Ding, Jeak L.
Site-directed mutagenesis of enhanced green fluorescent protein (EGFP) based on rational computational design was performed to create a fluorescence-based biosensor for endotoxin and gram-negative...
Immune Responses to Differentiated Forms of Helicobacter pylori in Children with Epigastric Pain
Ng, Bee Ling, Quak, Seng Hock, Aw, Marion, Goh, Kee Tai, Ho, Bow
Helicobacter pylori infection affects human populations of all ages. This gastric bacterium exists in spiral form and the reported viable but nonculturable coccoid form. The present study aims to...
Lui, Sook-Yin, Yeoh, Khay-Guan, Ho, Bow
The trend of increasing prevalence of antibiotic resistance among Helicobacter pylori strains has been suggested as a cause of the failure of treatment of H. pylori infections. In this study, 120 of...
The ancient origin of the complement system
Zhu, Yong, Thangamani, Saravanan, Ho, Bow, Ding, Jeak Ling
The complement system has been thought to originate exclusively in the deuterostomes. Here, we show that the central complement components already existed in the primitive protostome lineage. A...
Wang, Xiao Wei, Tan, Nguan Soon, Ho, Bow, Ding, Jeak Ling
The evolutionary conservation of the NF-κB transcription factors, from Drosophila to humans, underscores its pivotal role in immune response. Unexpectedly, the canonical NF-κB signaling pathway is...
Yau, Yin Hoe, Ho, Bow, Tan, Nguan Soon, Ng, Miang Lon, Ding, Jeak Ling
Factor C protein isolated from the horseshoe crab, Carcinoscorpius rotundicauda, has endotoxin binding capability. Synthetic peptides of 34 amino acids based on the sequence of two regions of factor...
A Novel Fluorescent Protein-Based Biosensor for Gram-Negative Bacteria
Goh, Yan Y., Ho, Bow, Ding, Jeak L.
Site-directed mutagenesis of enhanced green fluorescent protein (EGFP) based on rational computational design was performed to create a fluorescence-based biosensor for endotoxin and gram-negative...
Immune Responses to Differentiated Forms of Helicobacter pylori in Children with Epigastric Pain
Ng, Bee Ling, Quak, Seng Hock, Aw, Marion, Goh, Kee Tai, Ho, Bow
Helicobacter pylori infection affects human populations of all ages. This gastric bacterium exists in spiral form and the reported viable but nonculturable coccoid form. The present study aims to...
Lui, Sook-Yin, Yeoh, Khay-Guan, Ho, Bow
The trend of increasing prevalence of antibiotic resistance among Helicobacter pylori strains has been suggested as a cause of the failure of treatment of H. pylori infections. In this study, 120 of...
The ancient origin of the complement system
Zhu, Yong, Thangamani, Saravanan, Ho, Bow, Ding, Jeak Ling
The complement system has been thought to originate exclusively in the deuterostomes. Here, we show that the central complement components already existed in the primitive protostome lineage. A...
Wang, Xiao Wei, Tan, Nguan Soon, Ho, Bow, Ding, Jeak Ling
The evolutionary conservation of the NF-κB transcription factors, from Drosophila to humans, underscores its pivotal role in immune response. Unexpectedly, the canonical NF-κB signaling pathway is...
Elucidating the Function of an Ancient NF-κB p100 Homologue, CrRelish, in Antibacterial Defense▿ †
Fan, Ze Hua, Wang, Xiao Wei, Lu, Jinhua, Ho, Bow, Ding, Jeak Ling
The family of NF-κB transcription factors essentially regulates immune-related gene expression. Recently, we isolated and characterized the classical NF-κB/inhibitor κB (IκB) homologues from a...
C-reactive protein collaborates with plasma lectins to boost immune response against bacteria
Ng, Patricia M L, Le Saux, Agnès, Lee, Chia M, Tan, Nguan S, Lu, Jinhua, Thiel, Steffen, ...
Although human C-reactive protein (CRP) becomes upregulated during septicemia, its role remains unclear, since purified CRP showed no binding to many common pathogens. Contrary to previous findings,...
Local Inflammation Induces Complement Crosstalk Which Amplifies the Antimicrobial Response
Zhang, Jing, Koh, Jingyun, Lu, Jinhua, Thiel, Steffen, Leong, Benjamin S. H., Sethi, Sunil, ...
By eliciting inflammatory responses, the human immunosurveillance system notably combats invading pathogens, during which acute phase proteins (CRP and cytokines) are elevated markedly. However, the...