Brendan W. Wren

Re-annotation and re-analysis of the Campylobacter jejuniNCTC11168 genome sequence (2007)

Gundogdu, Ozan, Bentley, Stephen D, Holden, Matt T, Parkhill, Julian, Dorrell, Nick, Wren, Brendan W

Abstract Background Campylobacter jejuni is the leading bacterial cause of human gastroenteritis in the developed world. To improve our understanding of this important human pathogen, the C. jejuni...

The Complete Genome Sequence and Comparative Genome Analysis of the High Pathogenicity Yersinia enterocolitica Strain 8081 (2006)

Nicholas R. Thomson, Sarah Howard, Brendan W. Wren, Lisa Crossman, Gregory L. Challis, ...

The human enteropathogen, Yersinia enterocolitica, is a significant link in the range of Yersinia pathologies extending from mild gastroenteritis to bubonic plague. Comparison at the genomic level is...

Detection and Initial Characterization of Novel Capsular Polysaccharide among Diverse Campylobacter jejuni Strains Using Alcian Blue Dye

Karlyshev, Andrey V., Wren, Brendan W.

We have recently demonstrated that most strains of Campylobacter jejuni produce capsular polysaccharide (CPS), which can be detected by immunoblotting with homologous Penner antisera on...

A Novel Campylobacter jejuni Two-Component Regulatory System Important for Temperature-Dependent Growth and Colonization

Brás, Ana M., Chatterjee, Shimonti, Wren, Brendan W., Newell, Diane G., Ketley, Julian M.

Campylobacter jejuni colonizes the intestines of domestic and wild animals and is a common cause of human diarrheal disease. We identified a two-component regulatory system, designated the RacR-RacS...

Mutational Analysis of Genes Encoding the Early Flagellar Components of Helicobacter pylori: Evidence for Transcriptional Regulation of Flagellin A Biosynthesis

Allan, Elaine, Dorrell, Nick, Foynes, Susan, Anyim, Martin, Wren, Brendan W.

We investigated the roles of fliF, fliS, flhB, fliQ, fliG, and fliI of Helicobacter pylori, predicted by homology to encode structural components of the flagellar basal body and export apparatus....

Yersinia pestis pFra Shows Biovar-Specific Differences and Recent Common Ancestry with a Salmonella enterica Serovar Typhi Plasmid

Prentice, Michael B., James, Keith D., Parkhill, Julian, Baker, Stephen G., Stevens, Kim, Simmonds, Mark N., ...

Population genetic studies suggest that Yersinia pestis, the cause of plague, is a clonal pathogen that has recently emerged from Yersinia pseudotuberculosis. Plasmid acquisition is likely to have...

Local and Systemic Neutralizing Antibody Responses Induced by Intranasal Immunization with the Nontoxic Binding Domain of Toxin A from Clostridium difficile

Ward, Stephen J., Douce, Gill, Dougan, Gordon, Wren, Brendan W.

Fourteen of the 38 C-terminal repeats from Clostridium difficile toxin A (14CDTA) were cloned and expressed either with an N-terminal polyhistidine tag (14CDTA-HIS) or fused to the nontoxic binding...

Helicobacter pylori Possesses Two CheY Response Regulators and a Histidine Kinase Sensor, CheA, Which Are Essential for Chemotaxis and Colonization of the Gastric Mucosa

Foynes, Susan, Dorrell, Nick, Ward, Stephen J., Stabler, Richard A., McColm, Andy A., Rycroft, Andrew N., ...

Infection of the mucous layer of the human stomach by Helicobacter pylori requires the bacterium to be motile and presumably chemotactic. Previous studies have shown that fully functional flagella...

The Response Regulator PhoP Is Important for Survival under Conditions of Macrophage-Induced Stress and Virulence in Yersinia pestis

Oyston, Petra C. F., Dorrell, Nick, Williams, Kerstin, Li, Shu-Rui, Green, Michael, Titball, Richard W., ...

The two-component regulatory system PhoPQ has been identified in many bacterial species. However, the role of PhoPQ in regulating virulence gene expression in pathogenic bacteria has been...

Helicobacter pylori Pore-Forming Cytolysin Orthologue TlyA Possesses In Vitro Hemolytic Activity and Has a Role in Colonization of the Gastric Mucosa

Martino, M. Celeste, Stabler, Richard A., Zhang, Zun W., Farthing, Michael J. G., Wren, Brendan W., Dorrell, Nick

Hemolysins have been found to possess a variety of functions in bacteria, including a role in virulence. Helicobacter pylori demonstrates hemolytic activity when cultured on unlysed blood agar plates...

Demonstration of Polysaccharide Capsule in Campylobacter jejuni Using Electron Microscopy

Karlyshev, Andrey V., McCrossan, Maria V., Wren, Brendan W.

Recently, we reported that Campylobacter jejuni, an important gastrointestinal pathogen, has the genetic determinants to produce a capsular polysaccharide (Karlyshev et al., Mol. Microbiol....

Immunogenicity of a Salmonella typhimurium aroA aroD Vaccine Expressing a Nontoxic Domain of Clostridium difficile Toxin A

Ward, Stephen J., Douce, Gill, Figueiredo, Dayse, Dougan, Gordon, Wren, Brendan W.

The C-terminal repeat domain of Clostridium difficile toxin A harbors toxin-neutralizing epitopes and is considered to be a candidate component of a vaccine against C. difficile-associated disease...

Whole Genome Comparison of Campylobacter jejuni Human Isolates Using a Low-Cost Microarray Reveals Extensive Genetic Diversity

Dorrell, Nick, Mangan, Joseph A., Laing, Kenneth G., Hinds, Jason, Linton, Dennis, Al-Ghusein, Hasan, ...

Campylobacter jejuni is the leading cause of bacterial food-borne diarrhoeal disease throughout the world, and yet is still a poorly understood pathogen. Whole genome microarray comparisons of 11 C....

Application of DNA Microarrays to Study the Evolutionary Genomics of Yersinia pestis and Yersinia pseudotuberculosis

Hinchliffe, Stewart J., Isherwood, Karen E., Stabler, Richard A., Prentice, Michael B., Rakin, Alexander, Nichols, Richard A., ...

Yersinia pestis, the causative agent of plague, diverged from Yersinia pseudotuberculosis, an enteric pathogen, an estimated 1500–20,000 years ago. Genetic characterization of these closely related...

Adaptation of Campylobacter jejuni NCTC11168 to High-Level Colonization of the Avian Gastrointestinal Tract

Jones, Michael A., Marston, Kerrie L., Woodall, Claire A., Maskell, Duncan J., Linton, Dennis, Karlyshev, Andrey V., ...

The genome sequence of the human pathogen Campylobacter jejuni NCTC11168 has been determined recently, but studies on colonization and persistence in chickens have been limited due to reports that...

Explorative Multifactor Approach for Investigating Global Survival Mechanisms of Campylobacter jejuni under Environmental Conditions

Moen, Birgitte, Oust, Astrid, Langsrud, Øyvind, Dorrell, Nick, Marsden, Gemma L., Hinds, Jason, ...

Explorative approaches such as DNA microarray experiments are becoming increasingly important in microbial research. Despite these major technical advancements, approaches to study multifactor...

Intestinal Innate Immunity to Campylobacter jejuni Results in Induction of Bactericidal Human Beta-Defensins 2 and 3

Zilbauer, Matthias, Dorrell, Nick, Boughan, Parjeet K., Harris, Andrew, Wren, Brendan W., Klein, Nigel J., ...

Campylobacter jejuni is the most prevalent cause of bacterial diarrhea worldwide. Despite the serious health problems caused by this bacterium, human innate immune responses to C. jejuni infection...

Serotype Differences and Lack of Biofilm Formation Characterize Yersinia pseudotuberculosis Infection of the Xenopsylla cheopis Flea Vector of Yersinia pestis

Erickson, David L., Jarrett, Clayton O., Wren, Brendan W., Hinnebusch, B. Joseph

Yersinia pestis, the agent of plague, is usually transmitted by fleas. To produce a transmissible infection, Y. pestis colonizes the flea midgut and forms a biofilm in the proventricular valve, which...

Comparative phylogenomics of the food-borne pathogen Campylobacter jejuni reveals genetic markers predictive of infection source

Champion, Olivia L., Gaunt, Michael W., Gundogdu, Ozan, Elmi, Abdi, Witney, Adam A., Hinds, Jason, ...

Campylobacter jejuni is the predominant cause of bacterial gastroenteritis worldwide, but traditional typing methods are unable to discriminate strains from different sources that cause disease in...

Application of Comparative Phylogenomics To Study the Evolution of Yersinia enterocolitica and To Identify Genetic Differences Relating to Pathogenicity

Howard, Sarah L., Gaunt, Michael W., Hinds, Jason, Witney, Adam A., Stabler, Richard, Wren, Brendan W.

Yersinia enterocolitica, an important cause of human gastroenteritis generally caused by the consumption of livestock, has traditionally been categorized into three groups with respect to...

Detection and Initial Characterization of Novel Capsular Polysaccharide among Diverse Campylobacter jejuni Strains Using Alcian Blue Dye

Karlyshev, Andrey V., Wren, Brendan W.

We have recently demonstrated that most strains of Campylobacter jejuni produce capsular polysaccharide (CPS), which can be detected by immunoblotting with homologous Penner antisera on...

A Novel Campylobacter jejuni Two-Component Regulatory System Important for Temperature-Dependent Growth and Colonization

Brás, Ana M., Chatterjee, Shimonti, Wren, Brendan W., Newell, Diane G., Ketley, Julian M.

Campylobacter jejuni colonizes the intestines of domestic and wild animals and is a common cause of human diarrheal disease. We identified a two-component regulatory system, designated the RacR-RacS...

Mutational Analysis of Genes Encoding the Early Flagellar Components of Helicobacter pylori: Evidence for Transcriptional Regulation of Flagellin A Biosynthesis

Allan, Elaine, Dorrell, Nick, Foynes, Susan, Anyim, Martin, Wren, Brendan W.

We investigated the roles of fliF, fliS, flhB, fliQ, fliG, and fliI of Helicobacter pylori, predicted by homology to encode structural components of the flagellar basal body and export apparatus....

Yersinia pestis pFra Shows Biovar-Specific Differences and Recent Common Ancestry with a Salmonella enterica Serovar Typhi Plasmid

Prentice, Michael B., James, Keith D., Parkhill, Julian, Baker, Stephen G., Stevens, Kim, Simmonds, Mark N., ...

Population genetic studies suggest that Yersinia pestis, the cause of plague, is a clonal pathogen that has recently emerged from Yersinia pseudotuberculosis. Plasmid acquisition is likely to have...

Local and Systemic Neutralizing Antibody Responses Induced by Intranasal Immunization with the Nontoxic Binding Domain of Toxin A from Clostridium difficile

Ward, Stephen J., Douce, Gill, Dougan, Gordon, Wren, Brendan W.

Fourteen of the 38 C-terminal repeats from Clostridium difficile toxin A (14CDTA) were cloned and expressed either with an N-terminal polyhistidine tag (14CDTA-HIS) or fused to the nontoxic binding...

Helicobacter pylori Possesses Two CheY Response Regulators and a Histidine Kinase Sensor, CheA, Which Are Essential for Chemotaxis and Colonization of the Gastric Mucosa

Foynes, Susan, Dorrell, Nick, Ward, Stephen J., Stabler, Richard A., McColm, Andy A., Rycroft, Andrew N., ...

Infection of the mucous layer of the human stomach by Helicobacter pylori requires the bacterium to be motile and presumably chemotactic. Previous studies have shown that fully functional flagella...

The Response Regulator PhoP Is Important for Survival under Conditions of Macrophage-Induced Stress and Virulence in Yersinia pestis

Oyston, Petra C. F., Dorrell, Nick, Williams, Kerstin, Li, Shu-Rui, Green, Michael, Titball, Richard W., ...

The two-component regulatory system PhoPQ has been identified in many bacterial species. However, the role of PhoPQ in regulating virulence gene expression in pathogenic bacteria has been...

Helicobacter pylori Pore-Forming Cytolysin Orthologue TlyA Possesses In Vitro Hemolytic Activity and Has a Role in Colonization of the Gastric Mucosa

Martino, M. Celeste, Stabler, Richard A., Zhang, Zun W., Farthing, Michael J. G., Wren, Brendan W., Dorrell, Nick

Hemolysins have been found to possess a variety of functions in bacteria, including a role in virulence. Helicobacter pylori demonstrates hemolytic activity when cultured on unlysed blood agar plates...

Demonstration of Polysaccharide Capsule in Campylobacter jejuni Using Electron Microscopy

Karlyshev, Andrey V., McCrossan, Maria V., Wren, Brendan W.

Recently, we reported that Campylobacter jejuni, an important gastrointestinal pathogen, has the genetic determinants to produce a capsular polysaccharide (Karlyshev et al., Mol. Microbiol....

Immunogenicity of a Salmonella typhimurium aroA aroD Vaccine Expressing a Nontoxic Domain of Clostridium difficile Toxin A

Ward, Stephen J., Douce, Gill, Figueiredo, Dayse, Dougan, Gordon, Wren, Brendan W.

The C-terminal repeat domain of Clostridium difficile toxin A harbors toxin-neutralizing epitopes and is considered to be a candidate component of a vaccine against C. difficile-associated disease...

Whole Genome Comparison of Campylobacter jejuni Human Isolates Using a Low-Cost Microarray Reveals Extensive Genetic Diversity

Dorrell, Nick, Mangan, Joseph A., Laing, Kenneth G., Hinds, Jason, Linton, Dennis, Al-Ghusein, Hasan, ...

Campylobacter jejuni is the leading cause of bacterial food-borne diarrhoeal disease throughout the world, and yet is still a poorly understood pathogen. Whole genome microarray comparisons of 11 C....

Application of DNA Microarrays to Study the Evolutionary Genomics of Yersinia pestis and Yersinia pseudotuberculosis

Hinchliffe, Stewart J., Isherwood, Karen E., Stabler, Richard A., Prentice, Michael B., Rakin, Alexander, Nichols, Richard A., ...

Yersinia pestis, the causative agent of plague, diverged from Yersinia pseudotuberculosis, an enteric pathogen, an estimated 1500–20,000 years ago. Genetic characterization of these closely related...

Adaptation of Campylobacter jejuni NCTC11168 to High-Level Colonization of the Avian Gastrointestinal Tract

Jones, Michael A., Marston, Kerrie L., Woodall, Claire A., Maskell, Duncan J., Linton, Dennis, Karlyshev, Andrey V., ...

The genome sequence of the human pathogen Campylobacter jejuni NCTC11168 has been determined recently, but studies on colonization and persistence in chickens have been limited due to reports that...

Explorative Multifactor Approach for Investigating Global Survival Mechanisms of Campylobacter jejuni under Environmental Conditions

Moen, Birgitte, Oust, Astrid, Langsrud, Øyvind, Dorrell, Nick, Marsden, Gemma L., Hinds, Jason, ...

Explorative approaches such as DNA microarray experiments are becoming increasingly important in microbial research. Despite these major technical advancements, approaches to study multifactor...

Intestinal Innate Immunity to Campylobacter jejuni Results in Induction of Bactericidal Human Beta-Defensins 2 and 3

Zilbauer, Matthias, Dorrell, Nick, Boughan, Parjeet K., Harris, Andrew, Wren, Brendan W., Klein, Nigel J., ...

Campylobacter jejuni is the most prevalent cause of bacterial diarrhea worldwide. Despite the serious health problems caused by this bacterium, human innate immune responses to C. jejuni infection...

Comparative phylogenomics of the food-borne pathogen Campylobacter jejuni reveals genetic markers predictive of infection source

Champion, Olivia L., Gaunt, Michael W., Gundogdu, Ozan, Elmi, Abdi, Witney, Adam A., Hinds, Jason, ...

Campylobacter jejuni is the predominant cause of bacterial gastroenteritis worldwide, but traditional typing methods are unable to discriminate strains from different sources that cause disease in...

Serotype Differences and Lack of Biofilm Formation Characterize Yersinia pseudotuberculosis Infection of the Xenopsylla cheopis Flea Vector of Yersinia pestis

Erickson, David L., Jarrett, Clayton O., Wren, Brendan W., Hinnebusch, B. Joseph

Yersinia pestis, the agent of plague, is usually transmitted by fleas. To produce a transmissible infection, Y. pestis colonizes the flea midgut and forms a biofilm in the proventricular valve, which...

Application of Comparative Phylogenomics To Study the Evolution of Yersinia enterocolitica and To Identify Genetic Differences Relating to Pathogenicity

Howard, Sarah L., Gaunt, Michael W., Hinds, Jason, Witney, Adam A., Stabler, Richard, Wren, Brendan W.

Yersinia enterocolitica, an important cause of human gastroenteritis generally caused by the consumption of livestock, has traditionally been categorized into three groups with respect to...

The Complete Genome Sequence and Comparative Genome Analysis of the High Pathogenicity Yersinia enterocolitica Strain 8081

Thomson, Nicholas R, Howard, Sarah, Wren, Brendan W, Holden, Matthew T. G, Crossman, Lisa, Challis, Gregory L, ...

The human enteropathogen, Yersinia enterocolitica, is a significant link in the range of Yersinia pathologies extending from mild gastroenteritis to bubonic plague. Comparison at the genomic level is...