Bruce E. Ivins

Production of High Levels of Bacillus Anthracis Toxin Antigens in a New, Defined Culture Medium (2005)

Ristroph, Joseph D., Ivins, Bruce E.

Improved cultural conditions and a new, completely synthetic medium (R medium) were developed to facilitate the production of Bacillus anthracis holotoxin antigens. Levels of these antigens, up to...

Recent Advances in the Development of an Improved, Human Anthrax Vaccine (2005)

Ivins, Bruce E., Welkos, Susan L.

Human anthrax vaccines currently licensed in the United States and Western Europe consist of alum-precipitated or aluminum hydroxide-absorbed supernatant material from fermentor cultures of...

Anthrax Toxin (2003)

Leppla, Stephen H., Ivins, Bruce E., Ezzell, John W., Jr.

Anthrax toxin is a key virulence factor of Bacillus anthracis. The three protein components of the toxin have been purified and shown to have similar molecular weights: protective antigen (PA),...

Immunization against Anthrax with Aromatic-Dependent (Aro-) Mutants of Bacillus anthracis and with Recombinant Strains of Bacillus subtilis Producing Anthrax Protective Antigen (2003)

Ivins, Bruce E., Welkos, Susan L., Knudson, Gregory B., Little, Stephen F.

The safety and efficacy of five prototype, live anthrax vaccines were studied in Hartly guinea pigs and CBA/J and A/J mice. Two of the strains, Bacillus anthracis FD111 and FD112, are Aro-mutants...

Use of Dye-Ligand Affinity Chromatography for Separation of Toxin Components of Bacillus anthracis. (2002)

Little,Stephen F., Hedlund,Kenneth W., Ivins,Bruce E., Ristroph,Joseph D., Seburn,Dale W.

The protective antigen and lethal factor components of anthrax toxin were isolated from Bacillus anthracis culture supernatants and separated using dye-ligand affinity chromatography. The two...

The Search for a New-Generation Human Anthrax Vaccine (2000)

Ivins, Bruce E.

Anthrax is a disease primarily of herbivores, but humans can become infected through contact with infected animals or animal products. The etiological agent, Bacillus anthracis, possesses two primary...

Transponson Tn916 Mutagenesis in Bacillus anthracis, (1998)

Ivins, Bruce E., Welkos, Susan L., Knudson, Gregory B., LeBlanc, Donald J.

Mutagenesis of Bacillus anthracis by the streptococcal tetracycline resistance transponson, Tn916, is described. Tn916 was transferred from Streptococcus faecalis strain DS16C1 to B. anthracis VNR-1...

Influence of Body Weight on the Response of Fischer 344 Rats to Anthrax Lethal Toxin, (1998)

Ivins, Bruce E., Ristroph, J. D., Nelson, Gene O.

Groups of Fisher 344 rats were injected intravenously with Bacillus anthracis culture supernatants containing crude anthrax toxin. Times to death of rats given identical toxin preparations varied...

Short-Course Postexposure Antibiotic Prophylaxis Combined with Vaccination Protects Against Experimental Inhalational Anthrax (1998)

Vietri, Nicholas J., Purcell, Bret K., Lawler, James V., Leffel, Elizabeth K., Rico, Pedro, Gamble, Christopher S., ...

Prevention of inhalational anthrax after Bacillus anthracis spore exposure requires a prolonged course of antibiotic prophylaxis. In response to the 2001 anthrax attack in the United States, ~10,000...

Study of Immunization against Anthrax with the Purified Recombinant Protective Antigen of Bacillus anthracis

Singh, Yogendra, Ivins, Bruce E., Leppla, Stephen H.

Protective antigen (PA) of anthrax toxin is the major component of human anthrax vaccine. Currently available human vaccines in the United States and Europe consist of alum-precipitated supernatant...

Elaboration of Bacillus anthracis Antigens in a New, Defined Culture Medium

Ristroph, Joseph D., Ivins, Bruce E.

Improved culture conditions and a new, completely synthetic medium (R medium) were developed to facilitate the production of Bacillus anthracis holotoxin antigens. Levels of these antigens up to...

CpG Oligodeoxynucleotides Adsorbed onto Polylactide-Co-Glycolide Microparticles Improve the Immunogenicity and Protective Activity of the Licensed Anthrax Vaccine

Xie, Hang, Gursel, Ihsan, Ivins, Bruce E., Singh, Manmohan, O'Hagan, Derek T., Ulmer, Jeffrey B., ...

To reduce the biothreat posed by anthrax, efforts are under way to improve the protection afforded by vaccination. This work examines the ability of immunostimulatory CpG oligodeoxynucleotides (ODN)...

Isolation and Characterization of Melanin-Producing (mel) Mutants of Vibrio cholerae

Ivins, Bruce E., Holmes, Randall K.

Vibrio cholerae strain Htx-3, a hypertoxinogenic mutant of V. cholerae 569B Inaba, produces a dark brown pigment under certain growth conditions, whereas strain 569B does not. We investigated the...

Short-course postexposure antibiotic prophylaxis combined with vaccination protects against experimental inhalational anthrax

Vietri, Nicholas J., Purcell, Bret K., Lawler, James V., Leffel, Elizabeth K., Rico, Pedro, Gamble, Christopher S., ...

Prevention of inhalational anthrax after Bacillus anthracis spore exposure requires a prolonged course of antibiotic prophylaxis. In response to the 2001 anthrax attack in the United States,...

Study of Immunization against Anthrax with the Purified Recombinant Protective Antigen of Bacillus anthracis

Singh, Yogendra, Ivins, Bruce E., Leppla, Stephen H.

Protective antigen (PA) of anthrax toxin is the major component of human anthrax vaccine. Currently available human vaccines in the United States and Europe consist of alum-precipitated supernatant...

Elaboration of Bacillus anthracis Antigens in a New, Defined Culture Medium

Ristroph, Joseph D., Ivins, Bruce E.

Improved culture conditions and a new, completely synthetic medium (R medium) were developed to facilitate the production of Bacillus anthracis holotoxin antigens. Levels of these antigens up to...

CpG Oligodeoxynucleotides Adsorbed onto Polylactide-Co-Glycolide Microparticles Improve the Immunogenicity and Protective Activity of the Licensed Anthrax Vaccine

Xie, Hang, Gursel, Ihsan, Ivins, Bruce E., Singh, Manmohan, O'Hagan, Derek T., Ulmer, Jeffrey B., ...

To reduce the biothreat posed by anthrax, efforts are under way to improve the protection afforded by vaccination. This work examines the ability of immunostimulatory CpG oligodeoxynucleotides (ODN)...

Isolation and Characterization of Melanin-Producing (mel) Mutants of Vibrio cholerae

Ivins, Bruce E., Holmes, Randall K.

Vibrio cholerae strain Htx-3, a hypertoxinogenic mutant of V. cholerae 569B Inaba, produces a dark brown pigment under certain growth conditions, whereas strain 569B does not. We investigated the...

Short-course postexposure antibiotic prophylaxis combined with vaccination protects against experimental inhalational anthrax

Vietri, Nicholas J., Purcell, Bret K., Lawler, James V., Leffel, Elizabeth K., Rico, Pedro, Gamble, Christopher S., ...

Prevention of inhalational anthrax after Bacillus anthracis spore exposure requires a prolonged course of antibiotic prophylaxis. In response to the 2001 anthrax attack in the United States,...

Determination of Antibiotic Efficacy against Bacillus anthracis in a Mouse Aerosol Challenge Modelâ–¿

Heine, Henry S., Bassett, Jennifer, Miller, Lynda, Hartings, Justin M., Ivins, Bruce E., Pitt, M. Louise, ...

An anthrax spore aerosol infection mouse model was developed as a first test of in vivo efficacy of antibiotics identified as active against Bacillus anthracis. Whole-body, 50% lethal dose (LD50)...